EXPERIMENTAL ANIMALS
·
2022
·
IF 1.798
Kai KANG,Lukuan CUI,Qian ZHANG,Shijun GAO
Although many surgical or non-operative therapies have been developed to treat Achilles tendon injuries, the prognosis of which is often unsatisfactory. Recently, biologic approaches using multipotent stem cells like tendon-derived stem cells (TDSCs) pose a possible treatment option. To evaluate whether the Leucine rich repeat containing 32 (Lrrc32) affects the tenogenic differentiation of TDSCs and thus promotes Achilles tendon healing. TDSCs were infected with the recombinant Lrrc32-overexpressing lentivirus (LV-Lrrc32) and then locally injected into the injured site of rat. Four weeks after surgery, the Achilles tendon tissue (~0.5 cm) around the injured area was harvested for analysis. Pathological results showed that Lrrc32-overexpressing TDSCs significantly improved the morphological changes of the injured tendons. Specifically, the increased collagen-I expression and hydroxyproline content in extracellular matrix, and more orderly arrangement of the regenerated collagen fibers were observed in the Lrrc32 overexpression group. Moreover, 4 weeks after injection of Lrrc32-overexpressing TDSCs, the expression of tenocyte-related genes such as tenomodulin (Tnmd), scleraxis (Scx) and decorin (Dcn) were upregulated in the area of the healing tendon. These findings indicated that Lrrc32 promoted the tenogenic differentiation of TDSCs in vivo. Additionally, Lrrc32 overexpression also increased the expression of TGF-β1 and p-SMAD2/3, suggesting that the beneficial effects of Lrrc32 on tendon repair might be associated with the expression of TGF-β1 and p-SMAD2/3. Our findings collectively revealed that Lrrc32-overexpressed TDSCs promoted tendon healing more effectively than TDSCs alone.
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CARBOHYDRATE POLYMERS
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2022
·
IF 10.723
Hengfeng Zhu,Enwen Zhu,Yuanyuan Xie,Dongning Liu,Yang Hu,Zhuqun Shi,Chuanxi Xiong,Quanling Yang
Currently, microspheres with high adsorption capacity play a crucial role in dye adsorption and drug loading. In this study, 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibrils (TOCN) could be used to form nanocellulose microspheres by the emulsion method. The prepared hydrangea-like nanocellulose microspheres presented a stable three-dimensional network porous structure and exhibited excellent adsorption properties. The TOCN microspheres had a high adsorption capacity for methylene blue (MB) and methyl orange (MO) with the optimal adsorption capacity of 412.1 mg g −1 and 286.5 mg g −1 under neutral conditions, respectively. The TOCN microspheres displayed excellent adsorption selectivity on MB/MO mixed dyes, which could be used to selectively adsorb MB. Besides, the encapsulation rate of the positively charged drug doxorubicin hydrochloride (DOX) was as high as 93 %, and the drug loading capacity was as high as 34.5 %. Overall, our prepared nanocellulose microspheres had great potential for application in dye adsorption and drug delivery systems.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Ting Chen,Zhiliang Zhu,Xiaolin Shen,Hua Zhang,Yanling Qiu,Daqiang Yin
The construction of single Fe atoms with favorable electron structures is highly desired to boost peroxymonosulfate (PMS) activation for organics degradation. Herein, this study embedded isolated Fe atoms on N, O co-doped porous carbon substrate (Fe SA -N/O C), and first found the constructed FeN 4 O 1 configuration can realize efficient PMS activation via electron transfer by inner-sphere complexation, instead of the usual reactive oxygen species (ROS) as a major role. Structural investigation showed that the FeN 4 O 1 moieties possess high content of high spin (HS) Fe(II), resulting in the delocalization of unpaired electrons around Fe centers, which is benefit to transfer electrons when reacted with PMS. EIS and LSV curves further certified the electron transfer process. Density functional theory (DFT) calculations unveiled that the FeN 4 O 1 configuration can directly adsorb O O bond of PMS, leading to electron accumulation around Fe-O 2 bond, and then ulteriorly trigger the electron shuttling in organics degradation. Consequently, the optimized Fe SA -N/O C/PMS system exhibited superior oxidation ability for various organic pollutants, and was not affected by initial pH variation (3.19 ∼ 10.89), inorganic anions (ClO 4 − , Cl − and H 2 PO 4 − ) and natural organic matter (NOM) interference. Importantly, the developed system presented certain applicability in the treatment of actual water from Taihu Lake basin, China. Hence, this study not only elucidates the inner-sphere complexation-oriented electron transfer mechanism between unique FeN 4 O 1 configuration and PMS, but provides new insights into engineered single atom catalysts for wastewater purification.
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Nanomaterials
·
2022
·Vol. 12
·期 15
·2586
·
IF 5.719
Diyong Tang,Li Lu,Zhipeng Luo,Baokun Yang,Jun Ke,Weidong Lei,Hongran Zhen,Yuan Zhuang,Jie Sun,Ke Chen,Jie Sun
Biochars are considered as promising materials in energy storage and environmental remediation because of their unique physicochemical properties and low cost. However, the fabrication of multifunctional biochar materials with a well-developed hierarchical porous structure as well as self-doped functionalities via a facile strategy remains a challenge. Herein, we demonstrate a heteroatom-doped porous biochar, prepared by a hydrothermal pretreatment followed by a molten salt activation route. With the creation of a high specific surface area (1501.9 m2/g), a hierarchical porous structure, and the incorporation of oxygen-/nitrogen-functional groups, the as-prepared biochar (BC-24) exhibits great potential for supercapacitor application and organic pollutant elimination. The assembled biochar electrode delivers a specific capacitance of 378 F/g at 0.2 A/g with a good rate capability of 198 F/g at 10 A/g, and excellent cycling stability with 94.5% capacitance retention after 10,000 recycles. Moreover, BC-24 also exhibits superior catalytic activity for phenol degradation through peroxydisulfate (PDS) activation. The phenol (0.2 mM) can be effectively absorbed and then completely degraded within only 25 min over a wide pH range with low catalyst and PDS dosages. More importantly, TOC analysis indicates 81.7% of the phenol is mineralized within 60 min, confirming the effectiveness of the BC-24/PDS system. Quenching experiments and EPR measurements reveal that SO4·−and ·OH as well as1O2are involved in the phenol degradation, while the non-radical pathway plays the dominant role. This study provides valuable insights into the preparation of cost-effective carbon materials for supercapacitor application and organic contaminant remediation.Keywords:biochar;supercapacitor;organic pollutant;peroxydisulfate;free radicals;non-radical pathway
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Gels
·
2022
·Vol. 8
·期 8
·476
·
IF 4.432
Chengju Sheng,Xuemei Tan,Qing Huang,Kewen Li,Chao Zhou,Mingming Guo
Wounds, particularly under low-hydration conditions, require more time to repair successfully. Therefore, there is an urgent need to develop wound dressings that can accelerate wound healing. Hydrogels, which can maintain a moist environment around the wound and allow gas to pass through the material, act as antibacterial hydrogels as dressings and have great application value in the treatment of wounds. In addition, wound dressings (hydrogels) containing antibacterial capacity have lasting antibacterial effects and reduce damage to cells. In this work, we firstly synthesized two antibacterial agents: imidazolium poly(ionic liquids) containing sulfhydryl (Imidazole-SH) and ε-Poly(lysine) containing SH (EPL-SH). Then, lysine as a cross-linking agent, by “thiol-ene” click reaction, was mixed with Deferoxamine (DFO) to prepare the antibacterial hydrogels. The in vitro assays showed that the hydrogels could effectively killEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus). In addition, it also could reduce the inflammatory response produced by Lipopolysaccharide (LPS). More importantly, according to the transwell and angiogenesis assays, DFO-incorporated hydrogels promoted the migration and vascular repair of human umbilical vein endothelial cells (HUVECs). All the results revealed that the hydrogels provided new strategies for wound dressings.Keywords:antibacterial hydrogels;imidazolium poly(ionic liquids);vascular repair;anti-inflammatory;deferoxamine
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Yuxi Guo,Xuefeng Chen,Pin Gong,Jing Guo,Dan Deng,Guanglian He,Chenglong Ji,Ruotong Wang,Hui Long,Jiating Wang,Wenbo Yao,Wenjuan Yang,Fuxin Chen
We investigated the browning and softening of fresh Lentinula edodes (LE) coated with polysaccharides (LEP) isolated from LE stalks and stored at 4 °C for 15 days. The results showed that compared to the chitosan-coated and uncoated LE, the LEP-treated mushrooms showed significant improvements in several qualities during storage, such as reduced weight loss, retention of hardness and springiness, improved soluble protein content, and reduced browning, malondialdehyde content, and electrolyte leakage rate. The best results were obtained with 1.5 % LEP. LEP improved the activities of peroxidase, catalase, superoxide dismutase, ascorbate peroxidase, and phenylalanine ammonialyase and significantly reduced the accumulation of hydrogen peroxide during storage compared to the control samples. In addition, the LEP treatment maintained the high antioxidant activity of LE during storage. Notably, LEP inhibited browning-related enzymes (polyphenol oxidase and tyrosinase) to reduce browning. It also maintained high levels of cellulase, chitinase, and β -1,3 glucanase to improve softening during storage. These findings suggest the potential of LEP to improve the post-harvest quality of mushrooms, allowing a storage period of up to 15 days (extending the shelf life by six days) and indirectly suggesting that the polysaccharide component of LEP can act as a self-defense additive to protect against spoilage during storage.
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Advanced Science
·
2022
·Vol. 9
·期 27
·2202744
·
IF 17.521
Songtao Dong,Yuan Zhang,Xiangnan Guo,Chuang Zhang,Zhaomeng Wang,Jiang Yu,Yubo Liu,Chang Li,Yuting Hu,Bingjun Sun,Mengchi Sun,Haotian Zhang,Defang Ouyang,Zhonggui He,Yongjun Wang
Spatiotemporal delivery of nanoparticles (NPs) at the “cellular level” is critical for nanomedicine, which is expected to deliver as much cytotoxic drug into cancer cells as possible when NPs accumulate in tumors. However, macrophages and cancer-associated fibroblasts (CAFs) that are present within tumors limit the efficiency of spatiotemporal delivery. To overcome this limitation, glutathion pulse therapy is designed to promote reduction-sensitive Larotaxel (LTX) prodrug NPs to escape the phagocytosis of macrophages and penetrate through the stromal barrier established by CAFs in the murine triple negative breast cancer model. This therapy improves the penetration of NPs in tumor tissues as well as the accumulation of LTX in cancer cells, and remodels the immunosuppressive microenvironment to synergize PD-1 blockade therapy. More importantly, a method is established that can directly observe the biodistribution of NPs between different cells in vivo to accurately quantify the target drugs accumulated in these cells, thereby advancing the spatiotemporal delivery research of NPs at the “cellular level.”
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Weiwei Zhao,Yeke Yu,Zhiyuan Zhang,Dongmei He,Hongyu Zhang
The development of temporomandibular joint (TMJ) osteoarthritis is highly associated with mechanical overloading, which can result in accelerated cartilage degradation and damage due to increased interfacial friction and the release of inflammatory factors and catabolic enzymes. In the present study, we for the first time developed self-assembled drug-free nanospheres with pharmaceutical-active functions, which could be used as an intra-articularly injected biolubricant for the treatment of TMJ osteoarthritis based on a synergistic therapy of enhanced lubrication, anti-inflammation, and antisenescence. The nanospheres possessed the hydrophobic core of dopamine methacrylamide and the hydrophilic shell of sulfobetaine methacrylate, which formed into spherical aggregates in aqueous solution by specific interactions following reversible addition–fragmentation chain transfer polymerization. The biodegradation test, tribological test, and free radical scavenging test showed that the nanospheres were endowed with physiological stability, lubrication enhancement, and free radical scavenging capability. In addition, the in vitro cell test revealed that the nanospheres alleviated inflammatory and senescent phenotype for inflammation and oxidative stress stimulated chondrocytes. Furthermore, the in vivo animal test indicated that the nanospheres, after intra-articular injection into TMJ with an osteoarthritis environment, effectively protected condylar cartilage and subchondral bone from structural damage and attenuated cartilage matrix degradation and aging. In summary, the self-assembled nanospheres might be used as a promising biolubricant for achieving anti-inflammatory and antisenescent treatment of TMJ osteoarthritis.
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ACS Applied Nano Materials
·
2022
·
IF 6.14
Lei Cao,Mengyan Zhou,Jiaxi Wang,Qiang Zhu,Tingting Liu,Shushu Ding,Ding-Yi Fu
Gold–silver alloy nanoclusters (AuAgNCs) were synthesized, protected by glutathione S-transferase (GST). The as-prepared GST-AuAgNCs displayed strong (QY = 15.9%) fluorescence with two emission peaks around 477 and 653 nm under excitation of 395 nm. In this system, both the NC core and the organic shell contributed to the development of a “turn-on” ratiometric fluorescent probe for sensitively and selectively detecting oxytetracycline (OTC). First, the green fluorescence at 477 nm enhanced with the addition of OTC into GST-AuAgNC solution, whereas the red fluorescence at 653 nm remained unchanged. This “turn-on” ratiometric fluorescence may stem from the aggregation-induced emission effect and the reduction of Ag(I). The fluorescence ratio of GST-AuAgNCs displayed good linearity when the concentration of OTC was in the range of 0∼50 μM, and the detection limit was achieved down to 32.66 nM. Second, benefiting from the strong interaction between GST and OTC, the proposed method exhibited good anti-interference toward OTC in comparison with the common ions and natural amino acids. Moreover, the variation of ratiometric fluorescence intensity resulted in the corresponding red-to-green fluorescent color change, which could be employed to establish a simple paper-based analytical method for OTC colorimetric detection. Based on the superiority of high sensitivity and selectivity, as well as good accuracy and biocompatibility, the GST-AuAgNCs were successfully applied for detecting OTC in cells.
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Advanced Healthcare Materials
·
2022
·Vol. 11
·期 19
·2201323
·
IF 11.092
Shuang Xie,Kun Huang,Jiawen Peng,Yuan Liu,Wenxiong Cao,Dandan Zhang,Xiaohong Li
Diabetic foot ulcer (DFU) treatment is challenged by persistent bacterial infection and hyperglycemia-caused vascular dysplasia. Herein, self-propelled nanomotors are designed to achieve biofilm microenvironment (BME)-activated multistage release of NO for effective sterilization and subsequent angiogenesis promotion. CaO 2 nanoparticles (NPs) are capped with PDA layers, followed by complexation with Fe 2+ and surface grafting of cysteine-NO to obtain Janus Ca@PDA Fe -CNO NPs. In response to low pH in BME, the decomposition of CaO 2 cores generates O 2 from one side of Janus NPs to propel biofilm penetration, and the released H 2 O 2 and Fe 2+ produce •OH through Fenton reaction. The concurrent glutathione-triggered release of NO can be converted into reactive nitrogen species, which exhibit significantly higher bactericidal efficacy than those with only generation of •OH or NO. The slow release of NO for an extended time period promotes endothelial cell proliferation and migration. On Staphylococcus aureus -infected skin wounds of diabetic mice, NP treatment eliminates bacterial infections and significantly elevates blood vessel densities, leading to full wound recovery and regeneration of arranged collagen fibers and skin accessories. Thus, the self-propelling and multistage release of NO provide a feasible strategy to combat biofilm infection without using any antibiotics and accelerate angiogenesis and wound healing for DFU treatment.
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INVESTIGATIONAL NEW DRUGS
·
2022
·Vol. 40
·期 5
·977-989
·
IF 3.651
Duan Zelin,Zhou Zhiyun,Lu Feifei,Zhang Yawen,Guo Xvqin,Gui Chunshan,Zhang Hongjian
Targeting tumor metabolic vulnerabilities such as “glutamine addiction” has become an attractive approach for the discovery of novel antitumor agents. Among various mechanisms explored, SLC1A5, a membrane transporter that plays an important role in glutamine cellular uptake, represents a viable target to interfere with tumor’s ability to acquire critical nutrients during proliferation. In the present study, a stably transfected HEK293 cell line with human SLC1A5 (HEK293-SLC1A5) was established for the screening and identification of small molecule SLC1A5 inhibitors. This in vitro system, in conjunction with direct measurement of SLC1A5-mediated L-glutamine-2,3,3,4,4-D 5 (substrate) uptake, was practical and efficient in ensuring the specificity of SLC1A5 inhibition. Among a group of diverse compounds tested, mianserin (a tetracyclic antidepressant) demonstrated a marked inhibition of SLC1A5-mediated glutamine uptake. Subsequent investigations using SW480 cells demonstrated that mianserin was capable of inhibiting SW480 tumor growth both in vitro and in vivo, and the in vivo antitumor efficacy was correlated to the reduction of glutamine concentrations in tumor tissues. Computational analysis revealed that hydrophobic interactions between SLC1A5 and its inhibitors could be a critical factor in drug design. Taken together, the current findings confirmed the feasibility of targeting SLC1A5-mediated glutamine uptake as a novel approach for antitumor intervention. It is anticipated that structural insights obtained based on homology modeling would lead to the discovery of more potent and specific SLC1A5 inhibitors for clinical development.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Kairong Yang,Pei Jia,Jinjie Hou,Shuang Zhao,Li Wang
To achieve facile and rapid detection of tetracyclines (TCs), herein, we fabricated an ingenious turn-on ratiometric fluorescence sensor (Ru@ZIF-8) based on embedding red-emitting Ru(bpy) 3 2+ into zeolitic imidazolate framework-8 (ZIF-8). With the introduction of TCs, Ru@ZIF-8 system held the impervious red fluorescence, and generated green fluorescence which originated from the interaction between ZIF-8 and TCs, thereby achieving ratiometric fluorescence strategy through turn-on response signal and stable reference signal. Moreover, the ratiometric response accompanied discernible color change from red to green-yellow, which facilitated detection by naked eyes. The developed sensor exhibited prominent specificity and sensitivity, with detection limits of 2.4, 4.2, 1.6 and 7.2 nM for tetracycline, chlortetracycline , oxytetracycline and doxycycline , respectively. In addition, the satisfactory recoveries were obtained during detecting TCs in drink water, milk and beef, and the test paper-based sensor was successfully applied in real-time visual detection of TCs. All results indicated the feasibility and potential application of Ru@ZIF-8.
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