Frontiers in Chemistry
·
2022
·
IF 5.545
Yang Shuhui,Ke Xi,Zhang Menglong,Luo Dongxiang
Metal nanoparticles have been extensively used as co-catalysts in photocatalytic systems in order to pursue improvements in both reaction kinetics and selectivity. In this work, PdAg dual-metallic nanoparticles synthesized by the co-reduction method were decorated on a well-established α-Fe2O3/CdS Z-scheme photoactive material as a co-catalyst to study their performance for promoting the photoreduction of CO2. Herein, α-Fe2O3 and CdS were in situ synthesized on fluorine-doped tin oxide (FTO) glass by hydrothermal and SILAR (successive ionic layer adsorption and reaction) methods, respectively. The direct Z-scheme charge transfer path between Fe2O3 and CdS and the effective electron migration toward the PdAg mainly contributed to the excellent photocatalytic CO2 reduction performance. The controllable work function based on Pd (5.12) and Ag (4.26) constructed an appropriate band alignment with α-Fe2O3/CdS and displayed favorable production for CH4 rather than CO. The optimum ratio of PdAg 1:2 performed a 48% enhancement than pure Pd for photoreduction of CO2. Meanwhile, the enhanced charge separation improved the photoelectrochemical performance and photocurrent generation, and reduced the electrical resistance between components. This work provided insights into the dual-metallic co-catalyst for boosting the activity and selectivity of photocatalytic CO2 reduction.
研究领域:
材料科学
·
化学工程
·
环境科学
·
纳米技术
✓ 已验证
DOI
高影响因子
Aquaculture Reports
·
2025
·
IF 3.7
Hengyang Song,Xiangxiang Suo,Wanting Huang,Yubin Liu,Xiaojuan Huang,Wenshan Cai,Hao Liu,Beiping Tan,Xiaohui Dong
High-fat diets (HD) are extensively used in aquaculture due to saving feed cost, reducing protein level, and improve better fish growth and health conditions. However, the harmful impacts of HD, especially on health, limit application of HD in aquaculture. Due to the harmful effect of HD on hepatic health can be alternatively used α-lipoic acid and tea polyphenols, which exhibit great beneficial effect. This study investigated the effects of α-lipoic acid, tea polyphenols, and their combination on growth performance, antioxidant capacity, and inflammation-related indices in hybrid groupers ( ♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus ) fed a high-fat diet. A basic diet (CK group) with 50 % protein and 17 % lipid content was prepared. α-lipoic acid (LA) and tea polyphenols (TP) were used as additives, and 1000 mg/kg additive was added to the basic diet respectively, and the addition method was LA (LA group), TP (TP group), LA:TP = 1:1 (LA:TP group), LA:TP = 1:2 (LA:2TP group), LA:TP = 2:1 (2LA:TP group). Six experimental diets were fed to hybrid grouper (8.68 ± 0.22 g) for 8 weeks. These results showed that the addition of LA:TP = 1:2 (LA:2TP group) to the diet significantly increased final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR) ( P < 0.05). Compared with CK, LA, TP, LA:TP and 2LA:TP groups, the serum triglyceride (TG) content in the LA:2TP group was obviously decreased ( P < 0.05), and the ratio of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) was enhanced. In addition, the complements C3 and C4 contents in liver of the LA:2TP group were obviously increased, and the total cholesterol (TC) level was significantly decreased ( P < 0.05). Meanwhile, compared with the CK group, the serum contents of malondialdehyde (MDA) in each group were markedly reduced ( P < 0.05). The results of genes related to inflammation and antioxidation showed that compared with CK, LA, TP and LA:TP groups, the antioxidant genes ( sod , cat ) and anti-inflammatory cytokine genes ( tgf-β , il-10 ) in liver and head kidney of LA:2TP group were obviously up-regulated ( P < 0.05), while those in LA, TP and LA:TP groups were obviously greater than those in CK group ( P < 0.05), and the expression level in 2LA:TP group was the lowest ( P < 0.05). Correspondingly, the pro-inflammatory cytokine genes ( il-6 , tnf-α ) in the 2LA:TP group were significantly up-regulated and greater than those in other groups ( P < 0.05), and the expression level in the LA:2TP group was the lowest ( P < 0.05). In conclusion, adding LA:TP = 1:2 can effectively enhance growth performance, improve antioxidant capacity and reduce the harmful effects of HD diet in hybrid grouper.
研究领域:
水产养殖学
·
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
PROTEIN EXPRESSION AND PURIFICATION
·
2024
·
IF 1.4
Junzhang Chang,Yuxin Zhang,Zhiwei Li,Yunfeng Ma,Xueqin Hu,Jingwen Yang,Hongbin Zhang
Chiral amino acids and their deamination products, α-keto acids, have important applications in food, medicine, and fine chemicals. In this study, two l -amino acid deaminase genes from Proteus mirabilis , PM473 of type Ⅰ and PM471 of type Ⅱ were cloned and expressed in Escherichia coli respectively, expected to achieve the chiral separation of amino acids . Extensive substrate preference testing showed that both deaminases had catalytic effects on the d -amino acid component of the D, l -amino acids, and PM473 has a wider catalytic range for amino acids. When D, L-Cys was used as the substrate, all L-Cys components and 75.1 % of D-Cys were converted to mercapto pyruvate , and the remaining D-Cys was a single chiral enantiomer . Molecular docking analysis showed that the interaction between the substrate and the key residues affected the stereoselectivity of enzymes . The compatibility of hydrophobicity between the binding pocket and substrate may be the basic factor that affects the substrate selectivity. This work provides an alternative method for the production of α-keto acids and the resolution of chiral amino acids.
研究领域:
生物化学
·
生物催化
·
酶学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Yunxiao Zhang,Qingxiang Cao,Xuelian Wu,Yonghao Xiao,Aiyun Meng,Qitao Zhang,Yuxiang Yu,Wei-De Zhang
Establishing effective non-platinum active sites on polymeric carbon nitride (PCN) remains a great demanding to achieve highly efficient photocatalytic hydrogen generation performance. Modification with molecular complex catalysts on PCN skeletons has been demonstrated as a promising strategy. Herein, hybrid photocatalytic material was successfully synthesized by modifying PCN with copper molecular ligand ( CuL, Schiff-based copper complex) via π-π stacking. Interestingly, atomically dispersed Cu is formed in CuL/PCN. Compared to PCN, the π-π stacking in CuL/PCN is beneficial for facilitating the migration of photogenerated electrons from PCN to CuL . Furthermore, photo-redox ability of the atomically dispersed Cu is regulated by the ligand (Schiff base) in CuL and the introduction of CuL provides abundant active sites to capture the photogenerated electrons, whereby the reductive reaction of H 2 O/H + to H 2 for CuL/PCN was significantly promoted. Consequently, an optimal hydrogen evolution rate of 15.90 μmol·h −1 was obtained on 3CuL/PCN, which was 2.02 times higher than that of 3.0 wt% Pt/PCN (7.86 μmol·h −1 ). More importantly, the hybrid material exhibits an outstanding conversion efficiency (90 %) and selectivity (99 %) for α-methylation of ketones by making full use of the in-situ generated H species and photogenerated holes under visible light irradiation.
研究领域:
材料科学
·
催化
·
光化学
·
无机化学
✓ 已验证
DOI
高影响因子
VACUUM
·
2023
·
IF 4
Chaoqun Yan,Zhiliang Cheng,Xuan Zhang,Juan Wei,Qian Xu
The photocatalytic efficiency of hematite (α-Fe 2 O 3 ) is limited by its photoexcited state lifetime and diffusion length. By adjusting the surface structure, the photogenerated electron pairs can migrate to the surface and the charge recombination can be suppressed, which can effectively solve these problems and improve the photo-Fenton performance. In this study, four kinds different morphologies of α-Fe 2 O 3 - x (truncated hexagonal bipyramidal, cubic, hexagonal shape, and spherical) were synthesized by hydrothermal method , and a photocatalyst (H-α-Fe 2 O 3 - x ) with higher catalytic activity was prepared by hydrogenation. The results of UV–Vis DRS , PL , Raman, and XPS analysis , the oxygen vacancies and Fe (II) species were introduced into the α-Fe 2 O 3 catalyst after the hydrogenation reaction. The H-α-Fe 2 O 3 - x showed good degradation performance for TC and DX as EMPs model pollutants. The results show that the formation of oxygen vacancy and Fe 2+ is the main factor to improve the photocatalytic activity of the reaction system. Furthermore, the reaction mechanism revealed that reactive radicals involved in the H-α-Fe 2 O 3 - x photocatalytic system were •OH, •O 2 − , and h + responsible. This study provides an alternative technology for an easy and effective means for bandgap engineering to improve the photocatalytic performance of photocatalysts and facilitate widening environmental application.
研究领域:
环境科学
·
材料科学
·
化学
·
光催化
✓ 已验证
DOI
高影响因子
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
·
2018
·
IF 0.715
Ti Wu,Shoukai Lin,Bisha Wu,Yu Chen,Suying Lin,Dahe Lin,Linxia Chen,Zhisheng Zhang,Jincheng Wu
Cold-stored fruits of the loquat (Eriobotrya japonica Lindl.) plant suffer from strong lignification, which leads to a hard texture and a crude mouthfeel of the pulp. However, the signalling mechanisms of loquat fruits under cold storage remain unclear. In this study, the heterotrimeric G protein α subunit (Gα) gene (EjLGA1) from loquat was isolated, and in silico analysis was performed. The levels of EjLGA1 expression in cold-stored loquat fruits with different pretreatments were also investigated. The largest open-reading frame of EjLGA1 is 1173 bp, encoding a polypeptide of 390 amino acids. The nucleotide and amino acid sequences of the Gα subunit are highly conserved among loquat and 48 other seed plants, indicating that the loquat Gα subunit likely has similar functions in other plants. A reliable model of the loquat Gα subunit was constructed, which revealed the structure of the subunit. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results showed that EjLGA1 is highly expressed under cold storage, which suggests the significance of EjLGA1 function in loquat fruits in response to cold stress. However, ethephon and 1-methylcyclopropene (1-MCP) pretreatments did not regulate EjLGA1expression. These results provide new insight into the signalling mechanism in cold-stored loquat fruits.
研究领域:
植物学
·
分子生物学
·
食品科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Jing Tian,Feng Xiao,Yezhu Xu,Yuen Chen,Chenli He,Su Jiang,Yunping Tang
α-Monoglucosyl rutin (α-GR) is a structurally optimized rutin derivative, and its higher water solubility and bioavailability may help it exert better pharmacological effects in vivo . The present study examined the protective effects and potential mechanisms of α-GR on cyclophosphamide (CTX)-induced colonic injury in mice. The findings showed that α-GR significantly reduced serum levels of lipopolysaccharide (LPS) and diamine oxidase (DAO), effectively alleviating the histopathological damage in colon tissues caused by CTX exposure. Additionally, α-GR maintained the integrity of intestinal epithelial cells by promoting the secretion of secretory immunoglobulin A (sIgA) and upregulating the expression of tight junction proteins (Claudin-1 and ZO-1), while also reducing the levels of colonic pro-inflammatory factors and oxidative stress. In terms of gut microbiota, 16S rRNA gene sequencing and metabolomic analyses revealed that α-GR could effectively reverse the gut metabolic disorders and microbiota structural changes induced by CTX, promoted the growth of beneficial bacteria like norank_f_Muribaculaceae , Bacteroides, and Alloprevotella , and reduced the relative abundance of pathogenic bacteria ( Desulfovibrio ), while also revealing the complex correlation between them. In terms of mechanism research, α-GR further attenuated intestinal damage by regulating the TLR4/NF-κB and Nrf2/HO-1 pathways. This provides new ideas for the development of functional dietary supplements to treat CTX-associated intestinal toxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CNS Neuroscience & Therapeutics
·
2025
·Vol. 31
·期 5
·e70437
·
IF 5
Hai‐Qian Zhang,Yan Wang,Xiao Geng,Mingxin Dong,Ziwei Liu,Chengbiao Sun,Kaikai Yu,Wenwen Xin,Ye Xu,Na Xu,Wensen Liu
ANG‐modified liposomes encapsulating α‐melittin and resveratrol increase their blood–brain barrier penetration and GB cell selectivity. The combined treatment of α‐melittin with resveratrol markedly facilitates apoptosis and pyroptosis, and promotes the apoptosis–pyroptosis switch in the GB cells via downregulating the Wnt/β‐catenin signaling.
研究领域:
药理学
·
肿瘤学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2020
·
IF 4.335
Lin Chen,Jin He
A novel Michael/alkylation cascade reaction of N-unprotected 3-bromooxindoles with α,β-unsaturated acyl phosphonates using DABCO as a robust catalyst followed by the derivatization of the acyl phosphonate intermediates in situ has been developed. This scenario enables rapid access to a diverse set of highly functionalized spirocyclopropyl oxindoles in moderate yields with good to excellent diastereoselectivities, which are analogues of a high active non-nucleoside reverse transcriptase inhibitor against HIV-1. The synthetic potential of this tactic has been highlighted by a gram-scale reaction and Suzuki cross-coupling reactions of the product. Moreover, the reaction mechanism has been tentatively elucidated by control experiments and dynamic high-resolution mass spectrometry studies, which indicates that the Michael/alkylation cascade reaction involves DABCO-derived α-substituted ammonium ylides.
研究领域:
有机化学
·
药物化学
·
抗病毒研究
✓ 已验证
DOI
PubMed
高影响因子
Synthetic and Systems Biotechnology
·
2025
·
IF 4.4
Wei Li,Di Liu,Linbo Gou,Shengfang Wu,Xiuwen Zhou,Tai-Ping Fan,Long Wang,Yujie Cai
(-)-α-Bisabolol, a valuable monocyclic sesquiterpene alcohol, has garnered significant attention in the pharmaceutical and cosmetic industries due to its remarkable anti-inflammatory, antibacterial, and skin-care properties. In this study, Serratia marcescens HBQA7 ( S. marcescens HBQA7), a non-model strain resistant to terpenoid toxicity, was used as the production host, and the expression intensities of different integration sites were screened. The complete (-)-α-bisabolol synthesis pathway was integrated into these sites, achieving a production titer of 3.5 g L −1 . On this basis, by knocking out competitive pathway genes (such as slaB and adhE ) and global regulatory factors ( arcA and iclR ), and introducing efficient glucose transport and activation ( glf and glk ), the shake flask fermentation titer was increased to 7.21 g L −1 . Through optimization of fermentation culture by orthogonal experiments and others, the titer was further increased to 9.90 g L −1 . Finally, through the fed-batch fermentation process conducted in a 50 L bioreactor, a titer of 102.3 g L −1 was achieved after 110 h of cultivation. The productivity reached 0.93 g L −1 h −1 . This study not only establishes the most efficient microbial production system for (-)-α-bisabolol reported to date, but also demonstrates the outstanding potential of S. marcescens as a chassis for terpenoid biosynthesis. It provides a novel strategy for the industrial production of high-value terpenoids.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Lingfeng Wu,Wenhui Wu,Yanzi Cai,Congfa Li,Lu Wang
Mango leaves (MLs) have many important medical values owing to its high contents of phytochemical compounds. Among them, phenolic compounds existing in MLs showed multiple pharmacological activities. However, there is a little information about the quality evaluation and discrimination of different varieties of MLs. In the present study, the chemical compositions of MLs were identified by using high performance liquid chromatography coupled with electrospray ionization-quadrupole-time of flight-mass spectrometry (HPLC-ESI-qTOF-MS/MS). Then, the quality of ten MLs varieties collected from a same plantation was assessed according to integrated HPLC fingerprinting coupled with multivariate analysis. The results revealed that Cui Yu (S5) showed the highest TPC/TFC and the strongest bio-activity, followed by Tai Long (S7) and Hong Bao Shi (S3). Among different HPLC fingerprinting, twenty compounds were selected as common characteristic peaks, and the similarity was within the range of 0.792−0.995. Meanwhile, these varieties were divided into three groups: G1 (S3, S5, S7, and S10), G2 (S1 and S4) and G3 (S2, S6, S8, and S9). Two discriminant functions with the discriminant rate near 100 % were constructed. Additionally, neomangiferin, mangiferin, kaempferol-3- O -rutinoside, isoquercitrin and quercetin were found to be the key compounds in quality evaluation of MLs varieties. Pearson correlation coefficient analysis results confirmed that these key compounds directly contributed to the antioxidant activity and α -glucosidase inhibitory ability of MLs. Importantly, the possible inhibitory mechanisms of these key compounds against α -glucosidase were preliminary clarified by in silico analysis, and the analysis results provide a theoretical basis for future development and utilization of mango leaves byproducts.
研究领域:
植物化学
·
药理学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Lirong He,Peng Zhou,Liping Wang,Mingyu Zhang,Qizhong Huang,Zhean Su,Xiaodong Wang,Ping Xu,Weijie Song,Renhao Zou
Improving the performance of anode materials is of great importance for lithium-ion batteries (LIBs). Transition metal selenides compositing carbon and germanium (Ge) have attracted extensive attention. In this work, an anode material that integrates Ge and α-MnSe in carbon nanofibers (CNFs) was first synthesized by heat treatment after electrospinning. The coupling of Ge with α-MnSe and carbon can cause the growth process of α-MnSe to occur inside the CNFs, which can buffer the volume change, refine the grains and avoid particles protruding from the surface of CNFs. In addition, the interconnected conductive network of Ge/α-MnSe@CNFs can provide fast diffusion channels for Li ions and improve the electrical conductivity . Importantly, there is a synergistic effect of Ge, α-MnSe, and CNFs on the anode material for LIBs. As expected, Ge/α-MnSe@CNFs exhibited an excellent performance with a capacity of 1514.7 mAh g −1 after 200 cycles at 0.1 A g −1 and 1030 mAh g −1 after 1000 cycles even at 1 A g −1 . Therefore, this new preparation strategy of Ge/α-MnSe@CNFs offers novel ideas to develop high-performance and long-life LIBs.
研究领域:
材料科学
·
电化学
·
纳米技术
·
能源存储
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）