ACS Omega
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2018
·
IF 0
Wei Zhuang,Linjiao He,Kai Wang,Bo Ma,Lei Ge,Zhenfu Wang,Jinsha Huang,Jinglan Wu,Qi Zhang,Hanjie Ying
Developing targeted delivery nanosystems for delivering chemotherapeutic anticancer drugs specifically to cancerous tissues with improvement in the specificity of drugs for different cancer cells can result in high therapeutic efficacy and low toxicity in healthy tissues. Herein, we proposed the synthesis of a multifunctional nanodelivery system, folic acid (FA) decorating nanographene oxide (nGO) functionalized with poly(ethylene glycol) (PEG), called pGO-FA, with good biocompatibility and good delivering performance of a hydrophobic water-insoluble anticancer drug of paclitaxel (PTX). 4-br-PEG-NH2, FA, and PTX were attached to PEG-functionalized nGO (pGO) through a combined chemical and physical force to form a nanosized complex, pGO-FA-PTX, defined as the nanodrug system. WST-8 assay in vitro illustrated that pGO-FA-PTX inhibited A2780 cells in a concentration-dependent manner. Cell viability was kept high to 60% when treated with 200 nM of free PTX. However, pGO-FA-PTX with the same dose of PTX (cell viability less than 30%) had double the cytotoxicity effect compared to free PTX. Furthermore, fluorescence observation demonstrated that pGO-FA-PTX exhibited an improved efficiency in killing A2780 cells due to the special affinity between FA and FA receptor, which has high expression in cancer cells. The strategy and method used in this study could be effective in improving both the bioavailability of PTX and therapy efficiency.
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PubMed
NEW JOURNAL OF CHEMISTRY
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2018
·Vol. 42
·期 7
·4981-4990
·
IF 3.201
Min Wang,Ke-yan Zheng,Shao-wu Lv,Hai-feng Zou,Hong-sen Liu,Gang-lin Yan,Ai-dong Liu,Xiao-fang Fei
Besides having many important physiological functions, cell membrane proteins have been recognized as major markers for many diseases in clinical examination. The discovery of new cell membrane proteins has attracted significant research interest. Herein, a multifunctional Fe3O4@SiO2/CdTe nanocomposite was designed for the detection and separation of cell membrane proteins, which was synthesized by a covalent coupling reaction between amino groups and carboxyl groups. It can serve as a universal tool for modifying antigens or other molecules that have amino groups. To verify the feasibility and practicability of this method, a folic acid (FA)-functionalized magnetic/fluorescent nanoprobe (Fe3O4@SiO2/CdTe-NH-FA) has been developed for recognizing and separating the FA receptor on HepG2 cells. FA was fixed to the shell of Fe3O4@SiO2/CdTe nanocomposites via 1,6-hexamethylenediamine. It obviously reduces the fluorescence quenching effect of FA on magnetic/fluorescent nanocomposites. The results demonstrate that the synthesized nanoprobe Fe3O4@SiO2/CdTe-NH-FA provides excellent optical characteristics for the reliable imaging of cancer cells and has good magnetic properties to enrich and isolate cell membrane proteins. Consequently, this biological nanoprobe can be used to find, obtain, and study unknown membrane proteins and predict the occurrence of some diseases. In addition, this nanoprobe will be a potential platform for accurate cancer diagnosis and therapy in the future.
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高影响因子
MOLECULAR PHARMACEUTICS
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2018
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IF 4.556
Jiawang Guo,Xia Zhao,Jun Hu,Yuan Lin,Qian Wang
Cell-based ELISA (CELLISA) has been widely used in disease diagnosis due to its simplicity and low cost. Recently, peroxidase-like nanomaterials have emerged as promising systems for CELLISA applications. In this work, tobacco mosaic virus (TMV) was simultaneously tailored with peroxidase-like inorganic nanoparticles (platinum nanoparticles) and cancer cell target groups (folic acid, FA) to obtain TMV-FA-Pt nanoparticles for cancer cell detection. Induced by the uniformly distributed reactive groups and well-defined structure of the TMV particle, platinum nanoparticles could be grown in situ on the exterior surface of TMV with excellent monodispersity and uniform spatial distribution. Meanwhile, FA with a PEG1000 linker was successfully conjugated to the coat proteins of TMV through the Cu(I)-catalyzed alkyne–azide cycloaddition reaction, an efficient “click” chemistry. Our study demonstrated that the resultant TMV-FA-Pt had specific affinity to cancer cells and was successfully used to detect cancer cells through CELLISA. Less than 1.0 × 104 cells/mL of cancer cells could be readily detected.
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高影响因子
Applied Materials Today
·
2024
·
IF 7.2
Huahui Zeng,Man Li,Zhirong Wang,Tianqi Wang,Zhenqiang Zhang,Xiangxiang Wu
M1 macrophages play a crucial role in the development of rheumatoid arthritis (RA), which can produce various inflammation cytokines, and reactive oxygen species (ROS). Disulfide bonds as reduction-responsive linkages have been extensively employed in the drug delivery systems. However, disulfide bonds are rarely reported in the development of the oxidation-responsive drug carriers. Herein, we designed and synthetized novel oxidation-responsive drug carriers based on disulfide bonds, and further developed nanoparticles (FA-TP@NPs) by self-assembly for the RA treatment. FA-TP@NPs was decorated with folic acid and loaded with triptolide (TP), a potent anti-inflammation Chinese medicine, to target and enter M1 macrophages through the folate receptor on the cytomembrane for simultaneous repolarization of the M2 macrophage and elimination of the M1 macrophage. As expected, FA-TP@NPs exhibited an oxidation-responsive TP release via disulfide-bond oxidation and ester-bond hydrolysis in the presence of intracellular ROS, which accumulated preferentially in inflamed joints. FA-TP@NPs exerted a potent anti-arthritic effect with high biosafety in CIA mice, as evidenced by repolarizing M2 macrophages, inducing M1 macrophage apoptosis, and reducing TNF-α, IL-6, and IL-1β expression. This work shows that FA-TP@NPs give a deep insight into disulfide bond-based oxidation-responsive drug delivery systems, and provide a potential platform for RA treatment.
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高影响因子
ACS Applied Nano Materials
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2025
·
IF 5.5
Zhaoguo Wu,Xuezhi Zhou,Aiwu Zhao,Jin Wang
A combination of endoskeleton and exoskeleton in a dual shell has been considered for simultaneously improving the core–satellite binding efficiency and stability. A two-step preassembling-postgrowth approach has been considered for fabricating dual stimulus-responsive core–shell–satellite–shell (CSSS) nanocomposites. A CSSS therapeutic nanosystem has been designed for controllable release of the drug and in situ monitoring of chemotherapy via a dual stimulus-responsive mode. Incorporation of bifunctional SH-PNIPAM-COOH (TPNIC) in the nanocomposites plays a dual role in cross-linking of core–satellites and stimulus-responsive Raman enhancement. On the one hand, TPNIC in swelling status can act as a cross-linker for assembling Au satellites on the PFe3O4 (PEI-functionalized Fe3O4) core. On the other hand, TPNIC can adjust the assembling status of Au satellites via alteration of temperature; moreover, it induces optical hot-spot generation for giving a coupling plasmonic effect to produce a much stronger SERS performance than simple assembling. Thus, collapse of the TPNIC layer in the nanocomposites above the lower critical solution temperature (LCST) can be adopted for in situ enhanced monitoring chemotherapeutics of releasing the drug doxorubicin in a tumor microenvironment (TME). The dual stimulus-responsive therapeutic nanosystem can contribute to delivering the anticancer drug for efficiently penetrating the cell membrane and retarding release of the anticancer drug in TME. The obvious advantages of the therapeutic nanosystem are its facile magnetic recycling and regeneration so as to realize a sustainable chemotherapy of the tumor.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Yun Liu,Ziying Zhang,Yu Xia,Mengnan Ran,Qing Wang,Quanxin Wu,Wenhua Yu,Cao Li,Shiying Li,Ning Guo
In addition to tumor cells, M2-like tumor-associated macrophages (TAMs) also promote tumor progression. Accordingly, the strategy of targeted depletion or repolarization of M2-like TAMs becomes attractive. Here, we report a dual-targeting nanoagent SAMMH to tumor cells and M2-like TAMs for combinatorial tumor treatment. After co-loading the sonosensitizer spafloxacin (SPX) and oxidative phosphorylation inhibitor atavaquone (ATO) into hollow MnO 2 , the addition of Fe 3+ and tannic acid-immobilized hyaluronic acid (HA) caused the formation of SAMMH through generating metal-polyphenol networks (MPNs) coatings outside. In vitro endocytosis assays demonstrated the efficient internalization of SAMMH by both tumor cells and M2-like TAMs through the specific CD44-HA interactions. The GSH-sensitive degradation of SAMMH results in the continuous release of SPX and ATO. Meanwhile, SAMMH could catalyze the endogenous H 2 O 2 to extra O 2 , thus improving the therapeutic effect via the combination of Mn 2+ -induced CDT and O 2 -generation/O 2 -economy dual-enhanced sonodynamic therapy (SDT). Interestingly, SAMMH had a good targeted M2-like TAMs depleting capacity and could promote M2-to-M1 TAMs transformation by CDT-enhanced SDT, leading to a combinational anti-tumor effect. This dual-targeting nanoagent is a promising candidate to achieve CDT-enhanced SDT against both tumor cells and M2-like TAMs, thus providing new insights for the development of highly effective antitumor therapeutics.
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高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Lei Wang,Lei Xin,Yuchen Cao,Qi Xu,Jie Dong,Peixin Fan,Wenrun Hou,Qian Wang,Jian Meng,Ruiping Zhang,Jinfang Gao
Rheumatoid arthritis (RA) is a complex inflammatory disease of the joints, which is often accompanied by degeneration of articular cartilage and bone erosion, seriously affecting the quality of life and psychological state of patients. RA is difficult to be cured completely, and currently the main purpose of relief is through the use of anti-inflammatory and antirheumatic drugs, hormones, and biological agents. Tofacitib is a new type of small molecule inhibitor, which has a good effect in the treatment of RA. The current direct drug delivery method has serious side effects caused by the systemic distribution of the drug, so there is a need to develop an intelligent drug delivery system to realize precise treatment. In this work, tofacitib, gallic acid, targeted molecule folic acid, and Fe(III) were selected to assemble a novel type of artificial controllable nanodrug GF-TF. The self-photoacoustic/magnetic resonance imaging (self-PA/MRI) monitored the enrichment of GF-TF in the lesion in real-time, and artificially regulated the addition of deferoxamine (DFO) at the optimal enrichment. DFO strongly chelates Fe(III) in GF-TF and causes its structure to disintegrate gradually, and the self-PA/MRI signal of GF-TF became weaker while tofacitib began to be released, thus realizing the precise and artificially controlled release of the drug under the guidance of imaging. This nanodrug not only achieves efficient aggregation of drugs in inflamed joints, but also achieves real-time monitoring and precise control of drug release through self-PA/MRI, providing a new strategy for the precise treatment of RA.
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高影响因子
Aquaculture Reports
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2024
·
IF 3.2
Kangyuan Qu,Siyuan Xiong,Caiqian Huang,Xiaoxia Jiang,Lixia Tian,Shiwei Xie
Ornithine is an arginine family amino acid and is not incorporated into protein. This study evaluated its role in growth, immune response and acute NH 3 stress tolerance of Penaeus vannamei . Six diets with graded levels (0.6, 0.9, 1.2, 1.5, 1.8, 2.1 g kg −1 ) of L-ornithine were formulated, and fed the shrimp for 8 weeks. The growth of shrimp was significantly improved with the dietary ornithine levels increased from 0.6 to 1.5 g kg −1 . Feed efficiency, protein efficiency ratio and protein productive value showed a similar trend to the growth performance of shrimp. Whole body protein and muscle lipid of shrimp were significantly increased in shrimp fed 1.8 g kg −1 ornithine compared to those fed 0.6 g kg −1 ornithine. The contents of several amino acids were increased with the dietary ornithine increased to 1.5 g kg −1 . Elevated levels of ornithine increased the Nitric Oxide (NO) content and suppressed arginase activity in the hemolymph. The relative mRNA expression of superoxide dismutase , heat shock protein 70 , dorsal and caspase 3 were increased with the dietary addition of ornithine, while the mRNA level of arginase was decreased with the increasing dietary ornithine levels. After 9 h acute NH 3 stress, mortality of shrimp reduced with the increasing dietary ornithine levels. The relative mRNA levels of superoxide dismutase , heat shock protein 70 , dorsal and catalase were increased with the dietary addition of ornithine after the acute NH 3 stress. The second-order regression analysis of weight gain and survival after acute stress revealed that the suitable ornithine content in the shrimp diet was 1.4–1.5 g kg −1 .
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高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Lekang Liu,Mingbo Shao,Luoyuan Guo,Wenjun Wang,Xiuwen Zheng,Xiaolei Jiang
We have developed an innovative Cu-doped and DTX-loaded Cu-MnO2@DTX@FA (MCDF) nanodrug designed to strategically alter tumor microenvironment (TME) by harnessing the synergistic effects of chemodynamic therapy (CDT), chemotherapeutic agents, and the induction of ferroptosis and cuproptosis. The MCDF nanodrug efficiently degrades, releasing abundant Mn4+, Cu2+, and DTX. The conversion of Cu2+ to Cu+ facilitated by FDX1 initiates cuproptosis, while, similar to Mn2+, Cu+ reacts with hydrogen peroxide (H2O2) to generate hydroxyl radicals (·OH). Cu2+ and Mn4+ oxidize glutathione (GSH), significantly depleting GSH levels in tumor cells and inactivating GPX4, which further promotes ferroptosis. The release of Cu2+ and Mn4+ intensifies the cuproptosis. DTX effectively disrupts the cell division cycle, thereby inhibiting the proliferation and spread of tumor cells. The FA-modified MCDF is designed to evade immune detection while selectively targeting tumor tissues, ensuring precision in treatment delivery. This cutting-edge material not only provides a multifunctional therapeutic strategy but also sets the stage for the next generation of tumor-targeting nanomedicines.
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高影响因子
ANALYST
·
2024
·
IF 3.6
Zixuan Liao,Yuhui Wang,Yu Lu,Ruoxi Zeng,Lin Li,Hao Chen,Qingwei Song,Kaizhe Wang,Jianping Zheng
Phosphorescence analyses have attracted broad attention due to their remarkable merits of the elimination of auto-fluorescence and scattering light. However, it remains a great challenge to develop novel materials with uniform size and morphology, stability, long lifetime, and aqueous-phase room temperature phosphorescence (RTP) characteristics. Herein, monodisperse and uniform RTP nanobeads were fabricated by an in situ covalent hybridization of carbon dots (CDs) and dendritic mesoporous silicon nanoparticles (DMSNs) via silane hydrolysis. The formation of Si–O–C and Si–C/N covalent bonds is beneficial for the fixation of vibrations and rotations of the luminescent centers. Specially, the nanopores of DMSNs provide a confined area that can isolate the triplet state of CDs from water and oxygen and thus ensure the occurrence of aqueous-phase RTP with an ultra-long lifetime of 1.195 s (seen by the naked eye up to 9 seconds). Through surface modifying folic acid (FA), CDs@DMSNs can serve as a probe to distinguish different cell lines that feature varying FA receptor expression levels. In addition, taking MCF-7 as the model, highly sensitive and quantitative detection (linear range: 103–106 cells per mL) has been achieved via an RTP probe. Furthermore, their potential applications in cellular and in vivo time-gated phosphorescence imaging have been proposed and demonstrated, respectively. This work would provide a new route to design CD-based RTP composites and promote their further applications in the medical and biological fields.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-12
·
IF 5.3
Zhou Xiayu,Wang Yue,Song Jiayi,Xiong Lihao,Zhao Xin,Chen Sihan,Zhao Weichao,Li Le,Zhen Deshuai
Three types of carbon dots were synthesized using the same precursor (folic acid and europium nitrate) via different preparation methods (doping and direct coordination). A comprehensive comparison and analysis of the morphology, surface groups, and optical properties of the prepared carbon dots (CD), europium-doped carbon dots (CD-Eu), and europium-functionalized carbon dots (CD@Eu) were conducted. Moreover, due to the higher quantum yield, excellent stability, and outstanding selectivity for UO 2 2+ exhibited by CD-Eu, we selected CD-Eu as the probe for subsequent applications. CD-Eu showed a sensitive response to UO 2 2+ within the concentration range 25 ~ 200 nM, with a detection limit of 0.84 nM (0.42 μg·L −1 ). Additionally, CD-Eu demonstrated excellent accuracy and recovery in spiked detection of real water samples. Furthermore, we discovered that this probe could detect UO 2 2+ both in vitro and in viv o. This strategy provides a promising fluorescent sensor for the detection of UO 2 2+ in water and biological samples, holding significant implications for addressing UO 2 2+ contamination issues. Graphical abstract
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高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Guoquan Wu,Tianyu Su,Peng Zhou,Rongze Tang,Xu Zhu,Jin Wang,Minghao Chao,Liying Fan,Hanrong Yan,Peng Ye,Dehong Yu,Fenglei Gao,Hongliang Chen
Nanomedicines for immune modulation have made advancements in the treatment of rheumatoid arthritis (RA). However, due to aberrations in patients' immune systems, inducing antigen-specific immune tolerance while halting disease progression remains a significant challenge. Here, we develop a highly targeted multifunctional nanocomplex, termed M2Exo@CuS-CitP-Rapa (M2CPR), with the aim of selectively inhibiting inflammatory immune reactions while promoting immune tolerance towards specific antigens. M2CPR specifically targets inflammatory tissues in RA, delivering CuS NPs, CitP, Rapa, and endogenous anti-inflammatory factors, thereby ameliorating the inflammatory joint microenvironment. CuS NPs induce Cuproptosis of activated T cells, whose fragments are engulfed by resident or recruited macrophages, resulting in abundant production of TGF-β. TGF-β acts synergistically with Rapa to induce the iDCs into tDCs. tDCs present CitP to Naive T cells, promoting Tregs differentiation. Tregs, in turn, produce more TGF-β, inducing tDCs differentiation, thereby establishing a cycle of immune tolerance. Through in vitro and in vivo experiments, we validate that M2CPR can induce robust and durable antigen-specific immune tolerance, offering a new paradigm for RA therapy.
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高影响因子
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