Biochimica et Biophysica Acta-Gene Regulatory Mechanisms
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2018
·
IF 5.179
Quan Gan,Xinwei Zhang,Daobo Zhang,Liang Shi,Yue Zhou,Tongtong Sun,Song Jiang,Junshan Gao,Yan Meng
Sucrose is the most commonly transported sugar in plants and is easily assimilated by insects to fulfill the requirement of physiological metabolism. BmSuc1 is a novel animal β-fructofuranosidase (β-FFase, EC 3.2.1.26) - encoding gene that was firstly cloned and identified in silkworm , Bombyx mori . BmSUC1 was presumed to play an important role in the silkworm-mulberry enzymatic adaptation system by effectively hydrolyzing sucrose absorbed from mulberry leaves. However, this has not been proved with direct evidence thus far. In this study, we investigated sucrose hydrolysis activity in the larval midgut of B. mori by inhibition tests and found that sucrase activity mainly stemmed from β-FFase, not α-glucosidase. Next, we performed shRNA-mediated transgenic RNAi to analyze the growth characteristics of silkworm larvae and variations in glycometabolism in vivo in transgenic silkworms. The results showed that in the RNAi- BmSuc1 transgenic line, larval development was delayed, and their body size was markedly reduced. Finally, the activity of several disaccharidases alone in the midgut and the sugar distribution, total sugar and glycogen in the midgut, hemolymph and fat body were then determined and compared. Our results demonstrated that silencing BmSuc1 significantly reduced glucose and apparently activated maltase and trehalase in the midgut. Together with a clear decrease in both glycogen and trehalose in the fat body, we conclude that BmSUC1 acts as an essential sucrase by directly modulating the degree of sucrose hydrolysis in the silkworm larval midgut, and insufficient sugar storage in the fat body may be responsible for larval malnutrition and abnormal petite phenotypes.
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高影响因子
NPG Asia Materials
·
2018
·Vol. 10
·期 4
·177-189
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IF 7.208
Gao Zhong Feng,Sann Ei Ei,Lou Xiaoding,Liu Renyi,Dai Jun,Zuo Xiaolei,Xia Fan,Jiang Lei
Herein, we demonstrate a contact angle (CA)-based naked-eye point-of-care testing platform with rapid pH-responsive superwettability that can switch between superhydrophilic and superhydrophobic properties for quantitative biosensing. The CA of the droplet on the pH-responsive surface approached ~0° at pH 1 and conversely reached 161.4° ± 6.2° at pH 13. We realized the sensitive detection of the pH, urea, and glucose by monitoring the changes in the CA. The traditional invasive diagnosis of diabetes causes pain and brings the risk of infections, such as acquired immune deficiency syndrome (AIDS), hepatitis B, hepatitis C, and syphilis, to the user. To address this issue, we implemented a method for the non-invasive diagnosis of diabetes in human saliva and urine, which avoided the significant drawbacks mentioned above. The accuracy of this method was demonstrated by comparing the results with those from commercial glucometers and theoretical calculations. Interestingly, we successfully monitored glucose levels in sweat before, during, and after cycling. The sensing performance was barely influenced by the temperature, elevation, and droplet color, illustrating promise for expansion to hundreds of millions of potential customers, especially those with color blindness or color weakness. Given its low cost, lack of instruments, and rapid response (within 1 s), this strategy might overcome the limitations of the mechanical stability and durability of superwettable materials and thus might extend the industrial-scale application of bioinspired superwettable systems.
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ANALYTICA CHIMICA ACTA
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2018
·
IF 5.123
Ying Zhang,Wang Ren,Yu Zhu Fan,Jiang Xue Dong,Hui Zhang,Hong Qun Luo,Nian Bing Li
A simple and label-free fluorescence turn-on method is proposed for the discrimination and detection of epinephrine (Ep) and dopamine (DA) via polyethyleneimine (PEI)-initiated in situ copolymerization and excitation wavelength switch. The PEI solution in the presence of Ep, DA and the mixture of Ep and DA are denoted as P Ep-PEI , P DA-PEI and M Ep+DA , respectively. In this study, PEI aqueous solution medium initiates the auto-oxidization of Ep and DA and the bioinspired copolymerization. These resultant copolymers emit yellow-green fluorescence color with a fluorescence emission maximum at 515 nm. Interestingly, these fluorescent copolymers exhibit distinct different excitation spectra, although Ep and DA are structurally very similar. P DA-PEI exhibits only one excitation peak at 385 nm, and P Ep-PEI shows dual-excitation mode with two significant excitation peaks at 328 nm and 405 nm, respectively. M Ep+DA also shows dual-excitation mode with two excitation peaks at 330 nm and 395 nm, respectively. Thus, individual Ep, DA, and their mixture can be discriminated based on the different excitation spectral shapes and peak locations of P Ep-PEI , P DA-PEI and M Ep+DA . Furthermore, the quantitative analysis of Ep and DA in mixture can also be achieved by switching excitation wavelength between 330 and 395 nm and monitoring the fluorescence emission intensity of M Ep+DA at 515 nm. The fluorescence intensity of M Ep+DA only related to the concentration of Ep when excited at 330 nm. Moreover, the concentration of DA can also be calculated by subtracting the fluorescence intensity of P Ep-PEI from the total fluorescence intensity when excited at 395 nm. The resultant method has been used to simultaneously detect Ep and DA in human urine samples. The proposed fluorescence system is facile, eco-friendly, low-cost, and time-saving, and also provides a new and simple path for discriminating analogues.
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高影响因子
SENSORS
·
2018
·Vol. 18
·期 7
·2151
·
IF 2.475
Fei Qu,Jingwen Li,Wenli Han,Lian Xia,Jinmao You
Both adenosine triphosphate (ATP) and glucose are important to human health, and their abnormal levels are closely related to angiocardiopathy and hypoglycaemia. Therefore, the simultaneous determination of ATP and glucose with a single test mode is highly desirable for disease diagnostics and early recognition. Herein, a new fluorescence on/off switch sensing platform is developed by carbon nanodots (CNDs) to detect ATP and glucose simultaneously. The fluorescence of CNDs can be quenched by Cu2+and hydrogen peroxide (H2O2), due to the formation of hydroxyl radicals (·OH) produced in the Cu-Fenton reaction. Based on the high affinity of Cu2+with ATP, the fluorescence of CNDs will recover effectively after adding ATP. Additionally, glucose can be efficiently catalyzed by glucose oxidase (GOx) to generate H2O2, so the platform can also be utilized to analyze glucose. Under optimum conditions, this sensing platform displays excellent sensitivity and the linear ranges are from 0.1 to 7 μM for ATP with a limit of detection (LOD) of 30.2 nM, and from 0.1 to 7 mM for glucose with a LOD 39.8 μM, respectively. Benefiting from the high sensitivity and selectivity, this sensing platform is successfully applied for simultaneous detection of ATP and glucose in human serum samples with satisfactory recoveries.Keywords:simultaneous detection;Cu-Fenton reaction;carbon nanodots;adenosine triphosphate;glucose;satisfactory recoveries
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SENSORS
·
2018
·Vol. 18
·期 8
·2525
·
IF 2.475
Zhengjun Huang,Linlin Zheng,Feng Feng,Yuyuan Chen,Zhenzhen Wang,Zhen Lin,Xinhua Lin,Shaohuang Weng
Simple and effective methods for the detection of the level of blood glucose are closely linked to the monitoring of people’s health. In the study, MnO2nanosheets with absorption range of 300 nm~500 nm and obvious yellow color were easily prepared and applied to detect glucose through their absorbance and color. The proposed method is based on the fact that a specific concentration of glucose can be quantitatively transformed into hydrogen peroxide (H2O2) under the catalytic effect of glucose oxidase. Based on the redox reaction of MnO2with H2O2, yellow MnO2can be converted into colorless Mn2+to monitor the concentration of glucose. Under optimal conditions, a simple and effective visual assay for the sensitive and reliable detection of glucose was developed. The linear range was estimated to the range from 0 μM to 100 μM, with a detection limit of 12.8 μM. Furthermore, the proposed colorimetric assay based on MnO2nanosheets can effectively detect blood glucose of clinical serum samples with accuracy and convenience.Keywords:glucose;colorimetric assay;MnO2nanosheets;UV-vis;quantitative determination
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NANO
·
2018
·
IF 1.1
Bo Hu,Yang Lu,Keyu Cui,Yongsheng Yan
In this study, a novel imprinted polymer based on 3-aminophenylboronic acid (APBA)-functionalized CdTe quantum dots (QDs) was synthesized and used to sensitively and selectively detect glucose. In the process of synthesis, the boronic acid in the APBA could combine covalently with vicinal diol compounds, directing imprinting process, and the APBA-modified CdTe QDs were used as the solid supports. By this method, the prepared molecular imprinting polymers (MIPs)-APBA/CdTe QDs show high selectivity, high sensitivity and good stability. Under optimal conditions, a linear relationship was obtained covering the linear range of 0–1.5 mmol/L with a correlation coefficient of 0.99833 and a high imprinting factor about 5.71. Furthermore, the prepared MIPs-APBA/CdTe QDs were successfully applied to detect glucose in human serum samples. This work provides a new way to synthesize an excellent stability and efficient imprinted polymer based on CdTe QDs for convenient, fast and highly selective detection of glucose.
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JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 54
·期 4
·3144-3155
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IF 2.993
Qu Fei,Li Xiaonan,Lv Xiaoxia,You Jinmao,Han Wenli
Photoinduced electron transfer (PET) has been used as reporter in the field of photoluminescence sensors and switches, and intermolecular PET is believed to be responsible for fluorescence quenching. Herein, the emissions of Tb-MOF can be quenched effectively by protamine based on PET. Compared with conventional electrostatic attraction mechanism, this approach suffers less interference from other charged substances and has a broader linear range of protamine from 3 to 5000 ng/mL with limit of detection (LOD) 1.21 ng/mL. Subsequently, the strong binding effect of protamine toward Hep ascribed to electrostatic attraction hinders the interaction between Tb-MOF and protamine, resulting in the high emissions of framework. The detection of Hep also shows a good linear range from 0.5 to 5 μM with LOD 0.27 μM. Finally, this novel probe is also successfully applied to the determination of protamine and Hep in human serum with satisfactory results.
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APPLIED CATALYSIS A-GENERAL
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2018
·
IF 4.521
Zhi Cao,Ming Li,Yong Chen,Tao Shen,Chenglun Tang,Chenjie Zhu,Hanjie Ying
The widely available reagent ethylenediaminetetraacetic acid (EDTA) was used as a temperature-responsive catalyst to produce 5-hydroxymethylfurfural (HMF) from fructose. Using this catalyst, a remarkable HMF yield of 89 ± 3% was achieved in a water-MIBK biphasic system containing polyvinylpyrrolidone (PVP) and 2-butanol. The pH of the reaction system based on EDTA as a catalyst was shown to be temperature-responsive. Moreover, the EDTA catalyst could be recycled simply by cooling the reaction liquid to room temperature due to its temperature-dependent solubility in water. A slight decrease in the yield of HMF (from 89 ± 3 to 81 ± 2%) was observed after EDTA repeated use for five cycles, and a yield of 88 ± 2% was achieved again after purification of the recycled EDTA via recrystallization with NaOH/HCl. The FTIR spectrum of recycled EDTA was almost the same as that of fresh catalyst, and TG analysis showed the decomposition temperature of EDTA is about 260 °C. Furthermore, a leaching test revealed that only 4.3 ± 0.2% of the initial EDTA was lost after five-time reuse.
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ANALYST
·
2018
·Vol. 144
·期 1
·274-283
·
IF 3.864
Xin Xing Zhang,Qiu Yan Zhu,Jiao Yang Lu,Fu Rui Zhang,Wei Tao Huang,Xue Zhi Ding,Li Qiu Xia
Sensing of pyrophosphate (PPi) is helpful to better understand many life processes and diagnose various early-stage diseases. However, many traditional reported methods based on artificial receptors for sensing of PPi exhibit some disadvantages including difficulties in designing appropriate binding sites and complicated multi-step assembly/functionalization. Thus, it is significantly important and a big challenge to know how to use a simple molecular self-assembly or an interaction system to solve the above-mentioned limits to achieve the quantitative analysis of specific substances in the system. Based on the natural connection and similarity (such as stimulus responsiveness) between sensing and logic computing, in this study, the Boolean logic tree of molecular self-assembly system based on the cobalt oxyhydroxide (CoOOH) nanoplatform is constructed and applied to organize and connect “plug and play” molecular events (fluorescent dye, acridine orange and anion, PPi). By using molecules as inputs and the corresponding fluorescence signal as the output, the CoOOH-based molecular self-assembly system can be programmed for three-input fluorescent Boolean logic computation, fluorescent three-state logic computation, detection of PPi (linear range from 50 to 6400 nM with a detection limit of 20 nM) and even for imaging in living cancer cells and in vivo (in systems such as Zebrafish and Carassius auratus). Our approach adds a new dimension for expanding molecular logic computing and sensing systems, which will not only provide more opportunities for developing novel logic computing paradigms, but also be helpful in promoting the development and applications of intelligent molecular computing and sensing systems.
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TALANTA
·
2019
·
IF 4.916
Yaxue Jia,Shucheng Sun,Xiujun Cui,Xiaohong Wang,Li Yang
We show that vanadomolybdophosphoric heteropoly acid (H 5 PMo 10 V 2 O 40 , PMoV 2 ), one of the polyoxometalates (POMs), presents peroxidase-like activity that can catalyze the luminol/H 2 O 2 reaction to generate chemiluminescence (CL) emission. Based on this foundation, we report an enzyme-free luminol/H 2 O 2 /PMoV 2 CL system that can be utilized for the ultrasensitive detection of H 2 O 2 . The CL intensity exhibits good linear dependence on H 2 O 2 concentration in a wide range up to 5000 nM with a limit-of-detection of 5 nM. The method was successfully used for the determination of glucose in human serum. Our study paves the way for the application of functional POM materials in a variety of biosensing assays, such as the determination of cholesterol, uric acid , lactate, etc., in which H 2 O 2 is a product of oxidoreductase enzymes.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Lanfang Wang,Weiqi Zhu,Wenbo Lu,Lina Shi,Rui Wang,Ruixue Pang,YueYue Cao,Fang Wang,Xiaohong Xu
A novel nonsemiconductor photoelectrochemical biosensor was first constructed using the unique plasmonic AuNi nanodendrite arrays. The AuNi nanodendrite arrays were rapidly prepared by a one-step electrodeposition method using the porous anodic aluminum templates. Owing to its hierarchical structure with abundant active sites, the synergistic catalytic of Au and Ni can be better exploited. These plasmonic AuNi nanodendrite arrays display exceptional photoelectrocatalytic activities for glucose oxidation and hydrogen peroxide reduction reaction under visible light illumination. Specifically, the detection sensitivity for glucose (3.7277 mA mM −1 cm −2 ) under illumination is about 3.3 folds improvement than in the dark (1.1287 mA mM −1 cm −2 ), together with high accuracy and low detection limit of 3 μM. The markedly enhanced performance of AuNi nanodendrite arrays can be attributed to its hierarchical structure with abundant active sites and plasmonic effect of Au with strong absorption band in visible region. Such a newly developed method via the facile and low-cost route is of great significance in designing the plasmon-aided photoelectrochemical biosensors.
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Journal of Cleaner Production
·
2019
·
IF 6.395
Kuichuan Sheng,Shen Zhang,Jianglong Liu,Shuang E,Caidi Jin,Zenghua Xu,Ximing Zhang
Hydrochar microsphere as clean and facile carbon materials have great potentials to be used in biomass valorization for producing value-added chemicals. In this study, the hydrochars obtained from cellulose and xylan were prepared. The effect of hydrothermal conditions on hydrochars’ chemical composition, thermal stability and the porosity obtained from cellulose and xylan were evaluated. In order to explore the application of the hydrochar in biofuel production, the cellulose derived hydrochar supported Al catalyst (Al-Cel-HTC) was prepared by one-step hydrothermal process for catalysis glucose isomerization . We found distinct physicochemical properties between cellulose and xylan derived hydrochar. The specific surface area ( S BET ) of cellulose derived hydrochar (118 m 2 /g) is significantly higher than that of xylan derived hydrochar (28 m 2 /g). The hydrochars are composed of carbonaceous microspheres as evidenced by SEM. The catalytic performance of Al-Cel-HTC was investigated. Results shown that 77.8 mol/% selectivity of glucose to fructose can be obtained in aqueous by the catalyst. The SEM-EDS images of the catalyst shown the strong crystal-hydrochar microsphere interaction benifit in diminishing the agglomeration of hydrochar microsphere. The BET specific surface area and crystal structure (AlO(OH) and Al 2 O 3 ) of hydrochar supported aluminum catalyst have positive correlation with the catalytic selectivity.
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