REACTIVE & FUNCTIONAL POLYMERS
·
2025
·
IF 5.1
Tong Zhang,Weiyin Chen,Wenxin Zhang,Lusheng Liao,Chengpeng Li,Peng Zhang
Flexible electronic skin ( E -skin) demands materials with skin-like softness, self-healing capability, and antibacterial properties for long-term wearable applications. Here, we developed a series of self-healing, antibacterial zwitterionic polydimethylsiloxane (PDMS) elastomers via a facile photo-polymerization strategy. The elastomer was synthesized by crosslinking acrylate-terminated PDMS (PDMS-IU-MA) with zwitterionic sulfobetaine methacrylate (SBMA) using reactive hyperbranched polysiloxane (HPBSi) containing methacrylate groups as a multifunctional mediator. The resulting material exhibited remarkable antibacterial effects against E. coli and S. aureus , attributed to the zwitterionic SBMA moieties, while the dynamic hydrogen-bonding network formed by urethane/urea groups enabled self-healing ability. The mechanical properties, self-healing efficiency, and antibacterial performance were systematically tuned by varying the SBMA content. Furthermore, a conductive E -skin capable of precisely monitoring human motion through resistance changes was fabricated by integrating liquid metal (LM) microdroplets into an optimized PDMS matrix. This work provides a scalable strategy to integrate antimicrobial protection, self-healing, and strain sensing into E -skin for next-generation wearable electronics.
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高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Jia’nan Zhang,Zizhen Liu,Yihan Wang,Bo Yu
l-Homoserine is proven to be a promising precursor for the synthesis of l-phosphinothricin, which is a broad-spectrum herbicide with low toxicity. To enhance the sustainability of the process, the utilization of nonfood carbon sources for l-homoserine production is highly sought-after. Acetate, which can be captured from C1 gases, has the potential to become the most abundant feedstock for biomanufacturing. In this study, we systematically engineered the Escherichia coli strain to generate l-homoserine from acetate. The production of l-homoserine from acetate was initially achieved by deleting branch pathways, activating the glyoxylate shunt, and enhancing the synthetic pathway. The strain’s tolerance and acetate assimilation capabilities were strengthened through adaptive laboratory evolution. Reducing the flux toward gluconeogenesis further facilitated the production of l-homoserine. Finally, the engineered strain produced 15.96 g/L l-homoserine solely from CO2-derived acetate and formate in a 5 L fermentor via a fed-batch process. This study provides insights into the development of a low-carbon bioeconomy for the herbicide industry.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 9
·e70571
·
IF 3.4
Xiaoya Meng,Zhongyu Li,Zili Xia,Dongdong Chen,Zijing Yuan,Yang Han,Heng Zhang,Xia Sun,Jun Seop Lee,Usmanova Surayo Rakhmatzhanovna,Bobokalonov Jamshed Tolehmurodovich,Wenping Zhao
An electrochemical aptasensor based on Ti 3 C 2 T x (MXene) and cDNA-PAA was established for sensitive detection of aflatoxin B1 (AFB1) in peanuts. The high specific surface area of Ti 3 C 2 T x (MXene) contributed to improve electrochemical efficiency and enhancing sensor stability which were characterized by the Randles−Sevcik equation. The self-made polyacrylic acid (PAA) nanogel was added with aptamer complementary chain (cDNA) to form cDNA-PAA nano gel composite, which could realize the synergistic amplification of the difference between the electrochemical signals before and after the addition of AFB1. The sensing effect of cDNA-PAA was validated using DPV. Methylene blue (MB) was modified at the proximal 3′ termini of the aptamer (Apt-MB), meanwhile AuNPs was used to immobilize Apt-MB and improve the efficiency of electrochemical reactions. The competition between AFB1 and cDNA-PAA combined with Apt MB resulted in a significant change in the electrochemical signal current. In addition, the constructed electrochemical aptasensor had a lower detection limit of 1.0 × 10 −3 ng/L for AFB1 under optimal detection conditions, and a detection range of 1 to 1000 ng/L. Moreover, peanuts with different concentrations of AFB1 were used as actual detection samples. Through the constructed sensor detection, the spiked recovery rates within the spiking range of 50 to 500 ng/mL were 97.76% to 101.84% ( n = 3).
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PubMed
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Yonggui Song,Meixizi Lai,Zhou Liao,Zhentao Zhang,Genhua Zhu,Ming Yang,Zhifu Ai,Qin Zheng,Dan Su
Background Since the pathogenesis of depression is complex, antidepressant therapy remains unsatisfactory. Recent evidence suggests a link between depression and lipid metabolism. Saikosaponin (SS) exhibits antidepression and lipid-regulating effects in modern pharmacology. However, it is unknown whether lipid regulation is the key mechanism of the SS antidepressant effect and how it works. Purpose In this study, we investigated the relationship between the antidepressant activity of SS and the regulation of lipid metabolism and explored potential mechanisms. Methods APOE -/- mice, in combination with the chronic unpredictable mild stress (CUMS) model, were used to study the relationship between SS antidepressant activity and lipid metabolism through behavioral, electrophysiological techniques, and non-targeted lipidomics. Western blot, primary cell culture technology, and laser speckle cerebral blood flow imaging were employed to elucidate potential mechanisms. GraphPad Prism was used for statistical analysis, and p < 0.05 was considered statistically significant. Results APOE -/- mice exhibit more severe depressive-like behavior and dysregulation of sphingolipid metabolism in CUMS. SS alleviates depressive behavior and cortical sphingolipid metabolism disorder caused by CUMS, but has no effect on APOE -/- mice. SS alleviates the imbalance between ceramide (Cer) and sphingomyelin (SM) through acidic sphingomyelinase (AMSase). In addition, SS regulates neuronal glutamate release via sphingolipid metabolism, thereby alleviating the CUMS-induced inhibition of neurovascular coupling (regulates metabotropic glutamate receptor and IP3 receptor), which ameliorates the reduction of cerebral blood flow in depressed mice. Conclusion Our study highlights the role of lipid metabolism in the antidepressant activity of SS and explores its underlying mechanisms. This study provided new insights into the better understanding of the antidepressant mechanisms of phytomedicine while increasing the possibility of lipid metabolism as a therapeutic strategy for depression.
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PubMed
高影响因子
Theranostics
·
2017
·
IF 8.766
Jing-Bo Hu,Xu-Qi Kang,Jing Liang,Xiao-Juan Wang,Xiao-Ling Xu,Ping Yang,Xiao-Ying Ying,Sai-Ping Jiang,Yong-Zhong Du
The effective treatment for acute kidney injury (AKI) is currently limited, and care is primarily supportive. Sialic acid (SA) is main component of Sialyl Lewis x antigen on the mammalian cell surface, which participates in E-selectin binding. Therefore, dexamethasone(DXM)-loaded E-selectin-targeting sialic acid-polyethylene glycol-dexamethasone (SA-PEG-DXM/DXM) conjugate micelles are designed for ameliorating AKI. The conjugates are synthesized via the esterification reaction between PEG and SA or DXM, and can spontaneously form micelles in an aqueous solution with a 65.6 µg/mL critical micelle concentration. Free DXM is incorporated into the micelles with 6.28 ± 0.21% drug loading content. In vitro DXM release from SA-PEG-DXM/DXM micelles can be prolonged to 48h. Much more SA-PEG-DXM micelles can be internalized by lipopolysaccharide (LPS)-activated human umbilical vein endothelial cells (HUVECs) in comparison to PEG-DXM micelles due to specific interaction between SA and E-selectin expressed on HUVECs, and consequently more SA-PEG-DXM micelles are accumulated in the kidney of AKI murine model. Furthermore, SA in SA-PEG-DXM conjugates can significantly ameliorate LPS-induced production of pro-inflammatory cytokines via suppressing LPS-activated Beclin-1/Atg5-Atg12-mediated autophagy to attenuate toxicity. Compared with free DXM and PEG-DXM/DXM micelles, SA-PEG-DXM/DXM micelles show better therapeutical effects, as reflected by the improved renal function, histopathological changes, pro-inflammatory cytokines, oxidative stress and expression of apoptotic related proteins.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Lupeng Cui,Yumeng Qiu,Yu Liang,Chunjie Du,Weiliang Dong,Cheng Cheng,Bingfang He
The PET hydrolase from Ideonella sakaiensis ( Is PETase) is efficient for PET degradation, which provides a promising solution for environmental contamination by plastics. This study focuses on improving the excretion of Is PETase from E. coli by signal peptide (SP) engineering. A SP enhancer B1 (MERACVAV) was fused to the N-terminal of commonly-used SP (PelB, MalE, LamB, and OmpA) to mediate excretion of Is PETase. Strikingly, the modified SP B1OmpA, B1PelB, and B1MalE significantly increased the excretion of Is PETase, while Is PETase was basically expressed in periplasmic space without enhancer B1. The excretion efficiency of Is PETase mediated by B1PelB was improved by 62 folds compared to that of PelB. The hydrolysis of PET by crude Is PETase in culture solution was also enhanced. Furthermore, the amount of released MHET/TPA from PET by Is PETase was increased by 2.7 folds with pre-incubation of hydrophobin HFBII. Taken together, this work may provide a feasible strategy for the excretion and application of the Is PETase.
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PubMed
高影响因子
JOURNAL OF DAIRY SCIENCE
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2022
·
IF 4.225
Fan Yang,Xin Ma,Wei Hu,Ziyi Xiong,Meijiang Huang,Yong Wu,Xuanyi Meng,Zhihua Wu,Anshu Yang,Xin Li,Hongbing Chen
ABSTRACT Dairy processing can alter the digestion stability and bioavailability of cow milk proteins in the gastrointestinal tract. However, analysis of stable linear epitopes on cow milk allergens that could enter into intestinal mucosal is limited. Thus, this study aimed to investigate the digestion and transportation properties and residual allergen epitopes entering into gastrointestinal mucosa of 3 commercial dairy products, including pasteurized milk (PM), ultra-heat-treated milk (UHTM), and dried skim milk (DSM). In this work, the digestive stability of the 3 kinds of dairy products has been performed in a standard multistep static digestion model in vitro and characterized by Tricine-SDS-polyacrylamide gel electrophoresis and reversed-phase HPLC. With respect to gastrointestinal digestion in vitro, the main allergens including β-lactoglobulin (β-LG), α-lactalbumin (α-LA), and caseins were degraded gradually, and the resistance peptides remained in the PM with a molecular weight of range from 3.4 to 5.0 kDa. Simultaneously, the potential allergenicity of the cow milk proteins was diminished gradually and is basically consistent after 60 min of gastrointestinal digestion. After gastrointestinal digestion, the remaining peptides were transported via an Ussing chamber and identified by liquid chromatography-MS/MS. By alignment, 10 epitopes peptides were identified from 16 stable peptides, including 5 peptides (AA 92–100, 125–135, 125–138, and 149–162) in β-LG, 2 peptides in α-LA (AA 80–93 and 63–79), 2 peptides in α S1 -casein (AA 84–90 and 125–132), and 1 peptide (AA 25–32) in α S2 -casein were identified by dot-blotting mainly exist in UHTM and PM. This study demonstrates dairy processing can affect the digestion and transport characteristics of milk proteins and in turn alter epitope peptides release.
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DOI
PubMed
高影响因子
STAR Protocols
·
2023
·
IF 0
Zhen-Ping Zou,Yu-Hang Fan,Yue Du,Ting-Ting Fang,Wei Du,Ying Zhou,Bang-Ce Ye
Summary Engineered microorganisms hold potential for disease diagnosis and treatment. Here, we present a protocol to engineer E. coli Nissle 1917 strain (iROBOT) using genome insertion and plasmid construction to diagnose, record, and ameliorate inflammatory bowel disease in mice. We describe steps for constructing and administering iROBOT, diagnosing and recording colitis, preparing samples, and analyzing fluorescence and base editing ratios of iROBOT. We detail a colitis ameliorating assay using the disease activity index, colon length, tissue pathological section, and cytokine analysis. For complete details of the use and execution of this protocol, please refer to Zou et al. 1
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PubMed
Magnetic Resonance Letters
·
2026
·Vol. 7
·期 3
·200313
·
IF 2.6
Qiang Zhang,Ju Yang,Rong Yan,Yunfei Hu
G protein-coupled receptors (GPCRs) are pivotal therapeutic targets that signal through heterotrimeric G proteins. While structural insights into Gα s and Gα i/o subfamilies have advanced significantly, characterizing the Gα q/11 subfamily remains challenging due to poor soluble expression in prokaryotic systems, precluding high-yield isotope labeling needed for NMR studies. Here, we present an efficient expression strategy for Gα q protein in Escherichia coli . We identified the H5 helix as a critical determinant of folding and solubility. By swapping this helix with its Gα i/o counterpart and employing low-temperature co-expression with bacterial chaperones, we significantly enhance soluble yields while retaining GTPase activity. The 15 N-labeled protein exhibited well-dispersed 1 H– 15 N HSQC spectra, validating the suitability of this expression strategy for future high-resolution NMR investigations. Download: Download high-res image (183KB) Download: Download full-size image
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高影响因子
AICHE JOURNAL
·
2016
·Vol. 63
·期 6
·2212-2220
·
IF 2.98
Lei Qin,Jiangsheng Li,Hongye Cheng,Lifang Chen,Zhiwen Qi,Weikang Yuan
An innovative intensified vitamin E (VE) recovery process from the methylated oil deodorizer distillates (MODDs) was proposed, where VE was in situ transferred into a deep eutectic solvent (DES) with an organic salt. To design the process, the chlorine based quaternary ammonium salts were primarily investigated, and [N 4,4,4,4 ]Cl was selected as an association solvent which can efficiently form DES with α-tocopherol of the representative compound of VE. Based on the determined phase diagram of the DES freezing points, four phase regions were classified, and the effect of the [N 4,4,4,4 ]Cl/tocopherol ratio and temperature on the extraction performance and phase transformation was figured out. Moreover, an intensified association extraction process via in situ forming DES of α-tocopherol with [N 4,4,4,4 ]Cl was designed and validated by experiments. VE products were finally obtained from both model MODD (purity of 99.63%) and practical MODDs (purity of >79.18%), which verifies the excellent extraction efficiency for the proposed recovery method. © 2016 American Institute of Chemical Engineers AIChE J , 63: 2212–2220, 2017
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Yongkang Xi,Yuxiao Zou,Zhigang Luo,Liang Qi,Xuanxuan Lu
Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals. However, how to accurately control their in vivo release and stability is still challenging. Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE). The resulting dual-functional particle presented a uniform sheetlike shape and nanometer size. In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Benefiting from the antioxygenation of VE, lipid oxidation in the ODS-β-CD-VE-stabilized Pickering emulsion was effectively inhibited. Meanwhile, pH-induced protonation/deprotonation of carboxyl groups guaranteed that the emulsions kept steady at pH ≤4 but were unsteady under neutral conditions. In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment. This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.
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PubMed
高影响因子
CELLULOSE
·
2022
·Vol. 29
·期 8
·4661-4671
·
IF 6.123
Li Zhen,Wang Feifei,Liu Lin,Liu Yixin,Liu Jikai,Chen Xinxin,Yao Juming
Flexible wearable electronic textiles (E-textiles) are predicted to apply in the intelligence management of physical training for automatic, intelligent, and scientific health management. Recently, printing electronic is one of the attractive processes for the large-scale manufacturing of patterned E-textiles. However, it is an enormous challenge that achieve sensing functionality without sacrificing the original performance of fabric. Herein, we proposed a new scalable aerodynamic assisted screen print technology to develop highly sensitive, breathable, and durable electronic textiles via the integration of graphene ink. Briefly, stable graphene ink was fabricated based on the exfoliation and dispersion of cellulose/PEDOT:PSS solution, and directly screen printed on spandex/cotton blend fabric (SCBF) with the assisting of airflow pressure. The as-prepared ink infiltrated and wrapped along the fiber surface by aerodynamic assisted screen printing instead of filling fabric inter-fibers, endowing the SCBF with conductivity (82.16 Ω/sq), flexibility and breathability. Consequently, the acquired E-textile exhibited superior air permeability (38.5 L·m 2 /s), washing durability (> 50 cycles), excellent sensitivity (GF = 36), strain sensing performance and stability (> 9000 cycles). Finally, a monitoring smart garment has been demonstrated to show the potential of a large-scale E-textile for physical training or health management. Graphical abstract
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高影响因子
危险品化学品经营许可证（带存储）