Science China-Materials
·
2024
·
IF 6.8
Duan Yi,Lin Jiangtao,Yue Jiang,Li Yuanyuan,Liao Jinghan,Sun Ying,Wang Quan,Duan Yourong,Li Zhaojun
Atherosclerosis remains a major cause of morbidity and mortality worldwide. Intraplaque neovascularization critically promotes atherosclerotic progression and instability. Vascular endothelial growth factor A (VEGFA) stimulates aberrant microvessel growth in plaques by inducing endothelial cell proliferation and migration. Pigment epithelium-derived factor (PEDF) potently inhibits VEGFA-dependent neovascularization. This study introduces a thermosensitive hydrogel (PFSgel) developed from poloxamer 407 (F127) and sodium alginate (SA) to deliver PEDF locally to atherosclerotic lesions. The PFSgel demonstrated a suitable liquid-solid transition at body temperature (37°C), then forming a stable 3D network structure after SA gelling with the Ca 2+ in the physiological environment which contributed to the character of controlled release. Rheological analysis confirmed its phase transition temperature of 28.7°C and notable self-healing properties, making it ideal for dynamic vascular environments. In vitro experiments showed that PFSgel could suppress VEGFA-induced endothelial cells’ proliferation and migration through modulation of CD31 and MMP-2/MMP-9 signaling. Notably, in vivo degradation test validated the controlled release pattern of PFSgel. In Apoe-deficient atherosclerotic mice, ultrasound-guided PFSgel injection onto the abdominal aorta enabled gradual in situ release of encapsulated PEDF. This effectively reduced plaque burden, neovascularization, and luminal stenosis, even with exogenous VEGFA administration. Histological analyses confirmed reduced lipid deposition, plaque area, and neovascularization within plaques. Overall, this novel in situ -forming PEDF delivery platform enables targeted suppression of pathological neovascularization via CD31 and MMP-2/MMP-9 pathways, representing a promising approach to stabilize high-risk plaques by intervening against VEGFA-dependent neovascularization.
研究领域:
心血管疾病
·
药学
·
生物医学工程
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Yongli Shi,Weiwei Ma,Min Gao,Yaxing Yang
The purpose of this paper was to fabricate a novel methoxy poly(ethylene glycol)-block-poly(caprolactone)-block-poly(1, 4, 8-Trioxa [4.6] spiro-9-undecanone) (mPEG- b -PCL- b -PTOSUO, mPECT) triblock copolymer and study on the in vitro anti-tumor activity of curcumin-loaded mPECT nanoparticles (NPs). The mPEG- b -PCL- b -PTOSUO NPs were fabricated with solvent evaporation. Transmission electron microscope (TEM) and laser particle analyzer were applied to investigate the morphology and size distribution of the obtained mPECT NPs. The cytotoxicity of the copolymer (mPECT) was reflected by cell viability. Curcumin (CUR), as a model drug, was encapsulated into mPECT NPs. The in vitro anti-tumor activity of CUR-loaded mPECT NPs were also studied. 1 H nuclear magnetic resonance ( 1 H NMR), Raman, and Fourier transform infrared spectroscopy (FTIR) spectra confirmed the obtaining of mPECT. TEM photograph showed that most of mPECT NPs were in spherical shapes with a uniform size distribution. High cell viability suggested that the cargo-free mPECT NPs had no obvious cytotoxicity. Fluorescent photographs illustrated that CUR-loaded mPECT NPs could be up-taken by SW1990 cells. The medicated NPs could inhibit the proliferation of SW1990 cells. Therefore, the mPECT NPs could be used as a vehicle to improve the bioavailability and anti-tumor effects of CUR.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·
IF 2.8
Rui Gao,Shen Zhang,Hailong Liu,Zhizhou Yang,Yue Wu,Sheng Gao,Mei Li,Hanlin Shao,Mingjie Wu
Disposal of dye-contaminated wastewater has become an imperative issue. Synthesis of novel materials for larger-capacity removal of those contaminants assisting by special interaction is a significant challenge. In this paper, a series of hyper-crosslinked poly 9-bromophenanthrene hybrids (HCPs) were synthesized by combination of 9-bromophenanthrene (9BP) and octavinylsilsesquioxane (OVS) via Friedel-Crafts alkylation reaction. The synthesized HCPs were well characterized. The results illustrated that HCPs were porous polymer hybrids consisting of micropores and mesopores with the apparent surface areas ( S BET ) in range of 980.0–1010.6 m 2 g −1 , and the micropore and mesopore widths located at 1.42 and 4 nm. Dye adsorption disclosed that the adsorbed amounts of rhodamine B (RB) and Congo red (CR) on HCP-2 respectively arrived at 2646.0 ± 37.4 and 1550.0 ± 38.5 mg g −1 , ascribing to the interactions of pore filling, hydrogen bonds, π π stacking, electrostatic attraction, especially the C Br⋯π interaction between the resulting polymers and the dye molecules of RB and CR. Moreover, HCP-2 exhibited larger adsorbed amount of iodine vapor arriving at 179.9 ± 2.6 wt%. This work provided an innovative strategy to design new adsorbents for larger-amount removal of contaminants from the environment.
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JOURNAL OF SEPARATION SCIENCE
·
2026
·Vol. 49
·期 4
·e70404
·
IF 3.6
Jiye Tian,Xinyue Zheng,Zihan Tang,Chunpeng Feng,Meichen Liu,Ning Zhang,Hongyu Xue,Jiansong You,Lei Yin,Meiyun Shi
Polyethylene glycol (PEG) has emerged as a predominant synthetic polymer in contemporary drug delivery systems due to its unique physicochemical properties and versatile functionalization capabilities. Deciphering chain-length-dependent biosafety and cellular uptake profiles of PEG polymers is vital for optimizing nanomedicine formulations. We established an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) methodology employing ammonium adduction for multiplex quantification of PEG600 oligomers ( n = 9–18) in human hepatocellular carcinoma (HepG2) hepatocytes. The method demonstrated linear response ( R 2 > 0.99) across 10–1000 ng/mL for PEG600 oligomers ( n = 9–18). PEG600 oligomers ( n = 9–18) internalization in HepG2 cells was quantified by the validated UHPLC-MS/MS method. PEG600 polymers with 9–18 subunits could be absorbed into HepG2 cells with a small quantity (less than 0.04%). The cytotoxicity study suggested that the half-maximal inhibitory concentration of PEG600 on HepG2 cells is 31.42 mg/mL. PEG600 oligomers demonstrated negligible anti-proliferative effects in HepG2 hepatocytes. This study could provide analytical techniques and critical reference data for cytotoxicity and whole cellular uptake profiles of PEG600 polymers with 9–18 subunits in HepG2 cells. This work establishes an analytical framework for assessing the cytotoxicity and cellular uptake kinetics of PEG600 oligomers ( n = 9–18) in HepG2 cells.
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JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 12
·2528-2540
·
IF 2.557
Jinmao You,Kun Dou,Cuihua Song,Guoliang Li,Zhiwei Sun,Shijuan Zhang,Guang Chen,Xianen Zhao,Na Hu,Wu Zhou
A rapid, sensitive, and selective precolumn derivatization method for the simultaneous determination of eight thiophenols using 3-(2-bromoacetamido)- N -(9-ethyl-9 H )-carbazol as a labeling reagent by high-performance liquid chromatography with fluorescence detection has been developed. The labeling reagent reacted with thiophenols at 50°C for 50 min in aqueous acetonitrile in the presence of borate buffer (0.10 mol/L, pH 11.2) to give high yields of thiophenol derivatives. The derivatives were identified by online postcolumn mass spectrometry. The collision-induced dissociation spectra for thiophenol derivatives gave the corresponding specific fragment ions at m/z 251.3, 223.3, 210.9, 195.8, and 181.9. At the same time, derivatives exhibited intense fluorescence with an excitation maximum at λ ex = 276 nm and an emission maximum at λ em = 385 nm. Excellent linear responses were observed for all analytes over the range of 0.033–6.66 μmol/L with correlation coefficients of more than 0.9997. Detection limits were in the range of 0.94–5.77 μg/L with relative standard deviations of less than 4.54%. The feasibility of derivatization allowed the development of a rapid and highly sensitive method for the quantitative analysis of trace levels of thiophenols from some rubber products. The average recoveries ( n = 3) were in the range of 87.21–101.12%.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 30
·8339-8350
·
IF 3.637
Gao Yue,Tan Jiangkun,Lu Jiao,Sun Zhiwei,Li Zan,Ji Zhongyin,Zhang Shijuan,You Jinmao
In this study, a novel fluorescent labeling reagent 2-(9-acridone)-ethyl chloroformate (AEC-Cl) was designed, synthesized and applied for the determination of free amino acids by high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). The free amino acids were rapidly and efficiently labeled by AEC-Cl in the presence of basic catalyst (pH 9.0) within 5 min at room temperature (25 °C). The derivatives exhibited excellent stability and fluorescence properties, with maximum excitation and emission wavelengths at 268 nm and 438 nm, respectively. Derivatives of 22 kinds of natural amino acids were completely separated by gradient elution on a Hypersil ODS C18 column. Under the optimal conditions, the calibration curves exhibited excellent linear responses, with correlation coefficients of R 2 > 0.9994. The detection and quantification limits were in the range of 0.61–2.67 μg kg −1 and 2.07–8.35 μg kg −1 , respectively. Therefore, AEC-Cl was successfully applied for the detection of trace levels of free amino acids in honey samples.
A novel fluorescent labeling reagent was applied for the determination of free amino acids in honey by high-performance liquid chromatography with a fluorescence detector.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2017
·
IF 3.255
Yan Ren,Juanjuan Zhao,Yanan Shi,Caiyun Chen,Xiangming Chen,Changjun Lv
L-Hydroxyproline (L-Hyp) is an important biomarker for idiopathic pulmonary fibrosis (IPF). The quantitative methods based on high-performance liquid chromatography coupled with fluorescence detection after pre-column derivatization typically requires complicated derivatization conditions and obtains unstable derivatives. Here, a novel derivatization reagent, 9-acetylimidazol-carbazole, was synthesized for the first time to efficiently and rapidly label the amino groups of L-Hyp. The high-performance liquid chromatography method with pre-column derivatization was performed on an Agilent ZORBAX SB-C 18 column (4.6 × 250 mm, 5 μm). The product was measured using fluorescence detection at excitation and emission wavelengths of 232 and 370 nm, respectively. The method was validated in specificity, linearity, limit of detection (66.7 fmol), limit of quantification (333.3 fmol), intra-day precision (0.75%), inter-day precision (3.82%), stability (3.15%), and recovery (90.7–109.4%). The validated method was successfully applied to the determination of L-Hyp in the lung tissues of healthy and IPF rats. The results showed that the concentration of L-Hyp (3.64 mg/g) in the IPF model was significantly higher than the concentration (2.33 mg/g) in the healthy control group with P < 0.01. This is a new method for the determination of L-Hyp and can be used for other amino acid-related studies in the future.
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高影响因子
VETERINARY MICROBIOLOGY
·
2017
·
IF 2.628
Liying Lai,Jiao Dai,Huanyu Tang,Shouming Zhang,Chunyan Wu,Wancen Qiu,Chengping Lu,Huochun Yao,Hongjie Fan,Zongfu Wu
Streptococcus suis (SS), an important pathogen for pigs, is not only considered as a zoonotic agent for humans, but is also recognized as a major reservoir of antimicrobial resistance contributing to the spread of resistance genes to other pathogenic Streptococcus species. In addition to serotype 2 (SS2), serotype 9 (SS9) is another prevalent serotype isolated from diseased pigs. Although many SS strains have been sequenced, the complete genome of a non-SS2 virulent strain has been unavailable to date. Here, we report the complete genome of GZ0565, a virulent strain of SS9, isolated from a pig with meningitis. Comparative genomic analysis revealed five new putative virulence or antimicrobial resistance-associated genes in strain GZ0565 but not in SS2 virulent strains. These five genes encode a putative triacylglycerol lipase, a TipAS antibiotic-recognition domain protein, a putative TetR family transcriptional repressor, a protein containing a LPXTG domain and a G5 domain, and a type VII secretion system (T7SS) putative substrate (EsxA), respectively. Western blot analysis showed that strain GZ0565 can secrete EsxA. We generated an esxA deletion mutant and showed that EsxA contributes to SS virulence in a mouse infection model. Additionally, the antibiotic resistance gene vanZ SS was identified and expression of vanZ SS conferred resistance to teicoplanin and dalbavancin in Streptococcus agalactiae . We believe this is the first experimental demonstration of the existence of the T7SS putative substrate EsxA and its contribution to bacterial virulence in SS. Together, our results contribute to further understanding of the virulence and antimicrobial resistance characteristics of SS.
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高影响因子
NEUROCHEMICAL RESEARCH
·
2024
·Vol. 50
·期 1
·1-16
·
IF 3.7
Lao Dayuan,Gong Zhuowei,Li Taiyan,Mo Xuean,Huang Wen
The clinical manifestations of anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis may be closely related to the integrity of the blood–brain barrier (BBB). The P38 mitogen-activated protein kinase (P38MAPK) pathway plays a protective role in neurodegenerative diseases. However, whether the P38MAPK pathway is involved in the underlying mechanism of tight junction (TJ) protein disruption and neuronal damage has not been elucidated. Therefore, in this study, a mouse model of anti-NMDAR encephalitis was established by active immunization with NMDAR NR1 356-385 peptides. The critical pathways of P38MAPK were screened by interaction network and co-enrichment analysis. The role of P38MAPK pathways was investigated by the injection of P38MAPK inhibitor SB203580 (10 mg/kg, i.p.). Compared with the control group, the expression of occludin and zonula occludens (ZO)-1 in NMDAR NR1 356-385 group mice was downregulated, and the structure and function of BBB were damaged. However, after the intervention of SB203580, the activation of the P38MAPK was inhibited, the expression of matrix metalloproteinase 9 (MMP9) was reduced, and the function of BBB was improved. Meanwhile, inhibiting the P38MAPK pathway reversed the degradation of NMDAR NR1, while reducing the expression of the glial fibrillary acidic protein (GFAP) and pro-inflammatory factor tumor necrosis factor (TNF-α). It also relieved the damage of neuron-specific nucleus (NeuN), thus alleviating psychobehavioral symptoms. In conclusion, our results suggested that the P38MAPK pathway is involved in BBB destruction and neurobehavioral change in mice with anti-NMDAR encephalitis. Targeting the P38MAPK pathway may be a promising option for the treatment of anti-NMDAR encephalitis.
研究领域:
神经科学
·
免疫学
·
药理学
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Drug Design Development and Therapy
·
2017
·
IF 2.822
Jingrun Chen,Junjie Liu,Dongxiao Cui,Chaoqun Yan,Liqiang Meng,Liqian Sun,Shurong Ban,Rui Ge,Taigang Liang,Qingshan Li
This study deals with the design and synthesis of a series of novel 4-methoxy-substituted and 5-methyl-substituted (3′S,4′S)-(−)-cis-khellactones. The newly synthesized compounds were characterized by 1H nuclear magnetic resonance (NMR), 13C-NMR, mass spectrometry, and elemental analysis. All the derivatives were subjected to in vitro cytotoxicity screening against HEPG-2 (human liver carcinoma), SGC-7901 (human gastric carcinoma), and LS174T (human colon carcinoma), by using the MTT assay. The results revealed that several of the 4-methoxy-substituted compounds exhibited potent cytotoxicity. Among these, compound 12e showed the highest activity against cancer cells which 50% inhibitory concentration (IC50) values were in the range of 6.1–9.2 μM with low toxicity on normal human hepatocyte. Preliminary investigation of possible mechanisms of action of compound 12e against HEPG-2 cells indicated possible induction of apoptosis, as determined by morphological observations and Annexin V/propidium iodide (PI) double staining, in addition to apparent dissipation of mitochondrial membrane potential (MMP), as measured by 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1) staining in combination with the activation of caspase-9 and caspase-3 by Western blot analysis. Overall, the data suggest that compound 12e may be a promising potential anti-cancer agent that could act primarily by inducing apoptosis through the mitochondria-mediated intrinsic pathway in human hepatoma cells.
研究领域:
药理学
·
肿瘤学
·
药物化学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Meiling Wang,Guowen Meng,Qing Huang,Yilin Lu,Yao Gu
A prototype of fluorescence sensor, based on 1,4-dihydroxyanthraquinone (1,4-DHAQ) derivative and 9-fluorenylmethyl chloroformate (Fmoc-Cl) co-modified Fe 3 O 4 magnetic nanoparticals (MNPs) (denoted as 1,4-DHAQ/Fmoc-Cl co-modified Fe 3 O 4 MNPs), has been achieved via solvothermal synthesis of amine-functionalized Fe 3 O 4 MNPs and subsequent surface co-modification of 1,4-DHAQ derivative and Fmoc-Cl onto the Fe 3 O 4 MNPs. The 1,4-DHAQ/Fmoc-Cl co-modified Fe 3 O 4 MNPs can be used for not only simple rapid qualitative detection of Cu 2+ , Cd 2+ , Zn 2+ and Hg 2+ in aqueous solution, but also quantitative analysis of the above four heavy metal ions with lower detection limits of 8 × 10 −9 , 1.5 × 10 −8 , 10 −8 and 10 −8 M, respectively. This is the first prototype of fluorescence sensor that has potentials in the detection of Cu 2+ , Cd 2+ , Zn 2+ and Hg 2+ in aqueous solutions and even in drinking water . The sensor designing idea might be extended to other multifunctional chemosensors .
研究领域:
材料科学
·
环境化学
·
分析化学
·
纳米技术
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Lei Yu,Mengmeng Li,Qi Kang,Li Fu,Guizheng Zou,Dazhong Shen
Near-infrared electrochemiluminescence (NIR ECL) luminophores are strongly anticipated in ECL bioimaging and spectrum-resolved multiplexing assays. Herein, bovine serum albumin-stabilized Ag nanoclusters (BSA-Ag NCs) are demonstrated promising NIR ECL performance, exhibiting strong anodic ECL spectrum peak at 904 nm in aqueous medium . The ECL intensity of BSA-Ag NCs-triethanolamine system was enhanced 3.2 times by adding TiO 2 nanoparticles as co-reactant accelerator. In a spectrum-resolved triplex-color ECL multiplexing immunoassay , CdSe NCs, CdTe NCs and BSA-Ag NCs were used as the ECL tags, carbohydrate antigen 125 (CA125), carbohydrate antigen 19–9 (CA19-9) and cardiac troponin I (cTnI) were chosen as model analytes. The ECL peaks of three antigen-NCs pairs were simultaneously measured via one ECL potential scan. The composite of carbon nanotubes-TiO 2 nanoparticles-chitosan was served as the conductivity and co-reactant accelerators, enhancing the ECL intensity by 16 folds. With the integral intensity over the ECL peaks as the analytical signal to improve further the signal-to-noise ratio, the limits of detection were 0.035 mU mL −1 for CA125, 0.087 mU mL −1 for CA19-9, and 0.016 pg mL −1 for cTnI, respectively.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物医学工程
·
分子生物学
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高影响因子
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