FOOD CHEMISTRY
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2022
·
IF 9.231
Shan Wang,Mingfei Pan,Kaixin Liu,Xiaoqian Xie,Jingying Yang,Liping Hong,Shuo Wang
A molecularly imprinted sensor for highly sensitive and selective determination of dibutyl phthalate (DBP) was fabricated by combining multi-walled carbon nanotubes (MWCNTs) and Au nanoparticles (AuNPs) with surface molecularly imprinted polymer (SMIPs). The MWCNTs and AuNPs were designed to modify the electrode surface to accelerate the electron transfer rate and enhance the chemical stability. SMIPs were synthesized using SiO 2 microspheres as carriers. By loading SMIPs capable of identifying DBP on the surface of modified electrodes of MWCNTs and AuNPs, an electrochemical sensor for detecting DBP was successfully constructed. After optimizing the experimental conditions, the modified electrode SiO 2 -COOH@MIP/AuNPs/MWCNTs/GCE can recognize DBP in the range of 10 −7 g L −1 to 10 −2 g L −1 , and the detection limit achieved to 5.09 × 10 −9 g L −1 (S/N = 3). The results demonstrate that the proposed MIP electrochemical sensor may be a promising candidate electrochemical strategy for detecting DBP in complex samples.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Ke Cai,Wei Pi,Jiangyang Qin,Chenxi Peng,Dandan Wang,Yingying Gu,Yong Mei
In this study, an electrochemical immunosensor was constructed to detect the cytokeratin 19 fragment antigen 21-1 (CYFRA 21-1) in human serum. CYFRA 21-1 is the most sensitive tumor marker of non-small cell lung cancer (NSCLC), its content in normal human serum should be less than 3.3 ng/mL. When lung cancer cells dissolve or die, a myriad of CYFRA 21-1 is released into a tumor patient’s blood circulation , and its serum content elevates strikingly. Consequently, detecting CYFRA 21-1 by an electrochemical biosensor is expected to provide a new method for the early detection and prevention of lung cancer. In this study, a composite of UiO-66-NH 2 and carboxylated multi-walled carbon nanotubes (CMWCNTs) was used as the substrate material of a sensor; the resulting sensor had a large specific surface area and strong electrical conductivity . Moreover, gold nanoparticles (AuNPs) were used to bind to antibodies through an Au-S bonds. Also, a supersensitive detection of CYFRA 21-1 was achieved through the specific bindings of antigens and antibodies. Under optimal detection conditions, the change of current signal intensity of the immunosensor was proportional to the logarithm of CYFRA 21-1 concentration and had a linear relation in the range of 0.005–400 ng/mL, while the detection limit was 1.15 pg/mL (S/N = 3). The proposed immunosensor had high precision, stability, and selectivity . More importantly, the sensor was been successfully applied to detect CYFRA 21-1 in human serum with high recovery, providing a new method for early screening and dynamic monitoring of lung cancer.
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ACS Applied Nano Materials
·
2023
·
IF 5.9
Hui Zhong,Yonghua Yuan,Min Qing,Jianli Zuo,Yueyuan Li,Lijuan Bai
The extracellular domain of the human epidermal growth factor receptor (HER2-ECD) is a breast cancer biomarker. Accurate and sensitive detection of HER2-ECD concentration in serum is crucial in the diagnosis and patient follow-up. Herein, an “off–on” type electrochemiluminescence (ECL) aptasensor based on the resonance energy transfer (RET) between AuPt@ZIF-67 and AuNPs/g-C3N4/PDDA was developed for detecting HER2-ECD. First, AuNP-modified g-C3N4 (AuNPs/g-C3N4/PDDA), as a signal substrate, generates ECL signals under the co-reactant peroxodisulfate (S2O82–) in a specific potential range and shows significantly enhanced signal strength and stability. Then, AuPt@ZIF-67 expeditiously quenched the ECL signal of AuNPs/g-C3N4/PDDA because of ECL resonance energy transfer (ECL-RET) between AuPt@ZIF-67 and AuNPs/g-C3N4/PDDA. We designed a new aptasensor detection platform for HER2-ECD detection. After specific binding of the aptamer with HER2-ECD, the AuPt@ZIF-67 content in the system decreased, and the ECL response recovered. The biosensor demonstrated a linear range of HER2-ECD detection between 100 fg/mL and 100 ng/mL under ideal circumstances, with a low detection limit of 17.3 fg/mL. Furthermore, this biosensor was successfully tested in human serum samples. This strategy, as a novel ECL system with satisfactory stability and excellent sensitivity, can realize the detection of other biological molecules by changing the probe, which is a promising method.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2023
·
IF 4.3
Guona Li,Qingya Yang,Xiaoying Liu,Lvcheng Liu,Junshan Han,Xing Li
A very poisonous heavy metal Cd 2+ not only contaminates the living environment but also constitutes a serious menace to human health through water intake and food chains. Thus, monitoring and controlling the content of Cd 2+ with rapidity, sensitivity, and simplicity in tap water, soil, and rice samples is important especially. In the work, a high-efficiency sensor based on gold nanoparticles (AuNPs) embellished and refined with 3-(1H-Tetrazol-5-yl) aniline (TAA) for the colorimetric detection of Cd 2+ is rationally put forward. The interaction between Cd 2+ and TAA@AuNPs leads to the agglomeration of the AuNPs solutions, resulting in the color of the system changing from wine-red to gray-blue. The limit of detection (LOD) of the sensor is 1.0 μM when observe with the unaided eye, and UV–vis spectroscopy also has a LOD of 31 nM with an excellent linear relationship ( R 2 = 0.99049) in the concentration range of 0.0–8.0 μM for Cd 2+ , indicating that AuNPs modified with TAA can be effectively used for the quantitative determination of Cd 2+ . Furthermore, the colorimetric sensor has been tested on tap water, soil, and rice samples, and it shows promising potential for the practical monitoring of harmful Cd 2+ in environmental systems.
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ANALYTICAL LETTERS
·
2024
·
IF 1.6
Zixuan Wang,Ganghui Chu,Juan Peng,Shiyi Chen
Internal reference probes can effectively eliminate internal and external interferences. Hence, finding a suitable internal reference probe is the key to ratiometric electrochemistry. In this study, a simple and sensitive ratiometric electrochemical sensor for the determination of rutin was prepared for the first time using rhodamine B (Rh B) as an internal reference probe. The modified electrochemical sensor was constructed by electrodepositing gold nanoparticles (AuNPs) on a glassy carbon electrode followed by drop-coating chitosan-immobilized rhodamine B (CS@Rh B). Rh B has a dual function in that it has a stable electrochemical signal (1.18 V) (vs. Ag/AgCl) that is completely separated from the oxidation peak of rutin (0.52 V) to serve as an internal reference probe. At the same time, it synergistically enhances the electrochemical signal of rutin with AuNPs. When differential pulse voltammetry was used for the determination of rutin, the current ratio of IRutin/IRh B was employed as the signal for rutin determination with a wide linear range (0.1 to 70 μM) and a low detection line (0.028 μM). The recoveries for the determination of rutin in Coreopsis tinctoria and Buckwheat tea were 95.4%–106% and 95.3%–104%, respectively. This work successfully demonstrated the feasibility of Rh B as an internal reference probe.
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ELECTROCHIMICA ACTA
·
2021
·
IF 6.901
Cuizhu Ye,Xingguang Chen,Dong Zhang,Junjun Xu,Huiting Xi,Tiantian Wu,Danwen Deng,Chunhong Xiong,Jinsheng Zhang,Ganhui Huang
Coated with polypyrrole (Ppy)-based molecularly imprinted polymer (MIP), 3D CoFe-metal organic frameworks (CoFe-MOFs) with gold nanoparticles (AuNPs) was developed for electrochemical trace detection of norfloxacin (NOR). On one hand, the trace detection of NOR was realized with MIP/CoFe-MOFs/AuNPs/Glassy carbon electrode (GCE). The effective surface area of the imprinted cavities capable of identifying NOR on MIP/CoFe-MOFs/AuNPs/GCE increased to 0.1058 cm 2 after 3D CoFe-MOFs/AuNPs was modified, which indicates that CoFe-MOFs/AuNPs could generate more imprinted cavities, thus contributing to a lower limit of detection (LOD) of 0.131 pmol L −1 (S/N = 3) with a wider linear range (5–100, 100–1000 and 1000–6000 pmol L −1 ). On the other hand, the recognition reaction to NOR by MIP/CoFe-MOFs/AuNPs/GCE is an irreversible electrochemical oxidation reaction involving an electron and a proton, controlled by a mixed adsorption-diffusion mechanism. Rapid and precise detection of NOR in prescription (norfloxacin capsules with the specification of 0.1 g) and pure milk with the recovery rates between 97.04 and 110.2% and the relative standard deviations (RSD) were less than 5.7%. The measured results by MIP/CoFe-MOFs/AuNPs/GCE were close to the commercial norfloxacin enzyme-linked immunosorbent assay (ELISA) kit, demonstrates its high potential to NOR trace detection in environmental and animal foods.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Xiumei Zhuang,Yongjun Hu,Junjie Wang,Jieyu Hu,Qi Wang,Xingxing Yu
Tyrosinase (TYR) is as a well-known polyphenol oxidase and important biomarker of melanocytic lesions. Thus, developing powerful methods to determine TYR activity is of great value in the early diagnosis of skin disease. Direct surface-enhanced Raman scattering (SERS) detection of biomolecules is usually affected by non-specific interference and complicate structure of the analytes. It is a challenge to develop Raman-active molecules with specific recognition to analytes in complex media. Here, we report a novel colorimetric and surface-enhanced Raman scattering (SERS) dual-readout assay for the determination of TYR using commercially available and economical 4-mercaptophenyl boronic acid (4-MPBA) as a Raman-active and recognition molecule. 4-MPBA provides a unique interactive boronic acid group to the diol group of TYR substrate and exhibits good SERS signal. Also, the introduction of magnetic beads could promptly improve the anti-interference ability of dual-mode sensor. The TYR-incubated tyramine-modified magnetic beads could obviously change the concentration of 4-MPBA-AuNPs in the presence of O 2 and ascorbic acid , where the ultraviolet visible (UV–vis) absorption and SERS intensity were directly related to the concentration of TYR added. The dual-mode sensor had a rapid response to TYR within 1 min under optimized conditions and had high selectivity for TYR with a limit of detection at 0.001 U/mL. In addition, the dual-mode strategy showed promising prospects in the determination of TYR activity in serum samples and could be used to screen TYR inhibitors.
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高影响因子
TALANTA
·
2020
·
IF 5.339
Xuejiao Liu,Qiaoe Wang,Jun Chen,Xu Chen,Wensheng Yang
Highly sensitive determination of tumor exosomes is significant for early diagnosis of cancers and precision therapy. Herein, a sandwich peptide-based electrochemiluminescence (ECL) biosensor was developed for determination of phosphatidylserine (PS)-positive exosomes, a promising biomarker for early diagnosis of ovarian malignancy. A PS-specific binding peptide with high affinity was immobilized on Au nanoflowers (AuNFs) modified biosensing interface for recognition and capture of exosomes. Meanwhile, g-C 3 N 4 nanosheet loaded with luminol capped AuNPs (Lum-AuNPs@g-C 3 N 4 ) nanocomposite was used as the ECL signal nanoprobe. The g-C 3 N 4 nanosheets with large surface area were not only utilized as the carrier to immobilize more peptides for recognition of exosomes but also used to catalyze co-reactant H 2 O 2 decomposition to achieve the ECL signal amplification of luminol-H 2 O 2 system. Under optimal conditions, the biosensor showed superior performances compared with most currently available methods, including wider linear range across 5 orders of magnitude and a lower detection limit (LOD) down to 39 particles μL −1 . Moreover, the biosensor could be applicable for determination of exosomes in complex biological samples. This study indicates the combination of peptide recognition with nanoprobe as a label for signal amplification in sandwich ECL biosensing is a great promising strategy for sensitive and cost-effective determination of exosomes.
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Catalysts
·
2020
·Vol. 10
·期 6
·600
·
IF 3.52
Zui Tao,You Zhou,Nuo Duan,Zhouping Wang
Lead (II) is regarded as one of the most hazardous heavy metals, and lead contamination has a serious impact on food chains, human health, and the environment. Herein, a colorimetric aptasensor based on the graphene/Fe3O4-AuNPs composites with enhanced peroxidase-like activity has been developed to monitor lead ions (Pb2+). In short, graphene/Fe3O4-AuNPs were fabricated and acted as an enzyme mimetic, so the color change could be observed by chromogenic reaction. The aptamer of Pb2+was decorated on the surface of the amine magnetic beads by streptavidin–biotin interaction, and the complementary strands of the aptamer and target Pb2+competed for the binding Pb2+aptamer. In the presence of Pb2+, aptamers bonded the metal ions and were removed from the system by magnetic separation; the free cDNA was adsorbed onto the surface of the graphene/Fe3O4-AuNPs composites, thus inhibiting the catalytic activity and the color reaction. The absorbance of the reaction solution at 652 nm had a clear linear correlation with the Pb2+concentration in the range of 1–300 ng/mL, and the limit of detection was 0.63 ng/mL. This assay is simple and convenient in operation, has good selectivity, and has been used to test tap water samples, which proves that it is capable for the routine monitoring of Pb2+.Keywords:peroxidase;aptamer;graphene;enzyme mimetic;colorimetric method;food safety;rapid detection
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JOURNAL OF CLUSTER SCIENCE
·
2019
·Vol. 31
·期 2
·337-346
·
IF 2.125
Zhao Chenyu,Jiao Junfeng,Zhou Wencui,Zhang Yong,Liu Han,Yang Xiaogang,Pandi Boomi,Cai Yurong
In this study, a new HAP/rGO/AuNPs-AO modified carbon electrode (GCE) was fabricated for amperometric detection of ascorbic acid. The HAP/rGO/AuNPs composite was prepared by one-step hydrothermal method. The large specific surface area, strong adsorption and excellent biocompatibility of HAP nanowires enabled fast, stable and accurate detection of ascorbic acid. As array of quantum dot, AuNPs had strong electrocatalytic activity in addition to good adsorption to enzymes, which could provide high sensitivity and low limit of detection for biosensor. Then rGO ensured that current signal could be quickly transferred from the composite to electrode. Under the optimum conditions, the prepared biosensor demonstrated a wide linear range from 0.39 to 36 mM (R 2 = 0.99845), a low limit of detection (3.39 μM, S/N = 3) and a high sensitivity (15.949 mA moL −1 ) with good selectivity, stability (10.8%) and repeatability (3.9%), which might providing a reasonable and reliable way for ascorbic acid detection.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Shuang Zhao,Yuchan Zhang,Songtao Ding,Jingchuan Fan,Zhi Luo,Kunguo Liu,Qi Shi,Weichi Liu,Guangchao Zang
A reliable and non-invasive electrochemical immunosensor for nuclear matrix protein 22 (NMP22) was presented based on reduced graphene oxide-tetraethylenepentamine (rGO-TEPA) and gold nanoparticles‑platinum nanoparticles-metal organic frameworks (AuNPs-PtNPs-MOFs) nanomaterial . rGO-TEPA is a promising template to enhance the loading ability of electrode for its large specific surface area and abundance in amino groups. AuNPs-PtNPs-MOFs nanomaterial was synthesized by NaBH 4 reduction method and applied to immobilize biomolecules due to their good biocompatibility, well conductivity , and excellent H 2 O 2 electrocatalytic ability. The electrochemical response signal was primarily originated from the obstruction of immobilized NMP22 antigen towards electrocatalytic reduction of H 2 O 2 and the signal was recorded by differential pulse voltammetry . Under optimum conditions, the immunosensor showed a sensitive response towards NMP22 at a broad concentration range from 0.005 ng·mL −1 to 20 ng·mL −1 . Two strong linear ranges could be observed at 0.005 ng·mL −1 to 0.5 ng·mL −1 (R 2 = 0.9833), and 0.5 ng·mL −1 to 20 ng·mL −1 (R 2 = 0.9909), with a low LOD of 1.7 pg·mL −1 (S/N = 3). Satisfactory reproducibility, stability and selectivity were obtained for NMP22 detection in real urine samples compared with standard ELISA assay, demonstrating the good analytical performance and potential non-invasive clinical application of this label-free electrochemical immunosensor.
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BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Hongyan Zhang,Ling Lin,Xiaoxue Zeng,Yajuan Ruan,Yongning Wu,Minggui Lin,Ye He,FengFu Fu
We herein developed a novel biosensor for the visual detection of trace uranyl ion ( UO 2 2 + ) in aqueous environment with high sensitivity and specificity by using DNAzyme-functionalized magnetic beads (MBs) for UO 2 2 + recognition and gold nano-particles (AuNPs)-based enzymatic catalysis oxidation of TMB (3,3′,5,5′-tetramethylbenzidine sulfate) for signal generation. The utilization of MBs facilitates the magnetic separation and collection of sensing system from complex sample solution, which leads to more convenient experimental operation and more strong resistibility of the biosensor to the matrix of sample, and the utilization of AuNPs-based enzymatic catalysis amplification greatly improved the sensitivity of the biosensor. Compared with the previous DNAzyme-based UO 2 2 + sensors, the proposed biosensor has outstanding advantages such as relative high sensitivity and specificity, operation convenience, low cost and more strong resistibility to the matrix of sample. It can be used to detect as low as 0.02 ppb (74 pM) of UO 2 2 + in aqueous environment by only naked-eye observation and 1.89 ppt (7.0 pM) of UO 2 2 + by UV–visible spectrophotometer with a recovery of 93–99% and a RSD≤5.0% ( n =6) within 3 h. Especially, the visual detection limit of 0.02 ppb (74 pM) is much lower than the maximum allowable level of UO 2 2 + (130 nM) in the drinking water defined by the U.S. Environmental Protection Agency (EPA), indicating that our method meets the requirement of rapid and on-site detection of UO 2 2 + in the aqueous environment by only naked-eye observation.
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危险品化学品经营许可证（带存储）