FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Mengke Zhang,Mengyin Li,Yan Zhao,Naifeng Xu,Lanlan Peng,Yuanfeng Wang,Xinlin Wei
In this study, a rapid sandwich immunochromatographic assay (ICA) was developed to detect parvalbumin (PV). Firstly, two optimum primary monoclonal antibody (mAb) against PV had been screened out: mAb1 was used as the capture antibody, and mAb2 conjugated to Fe 3 O 4 /Au nanoparticles (Fe 3 O 4 /AuNPs) that served as a detection reagent. Using this pair of mAbs, a sandwich ICA strip based on Fe 3 O 4 /AuNPs was developed. The results showed that the color intensity of test line positively correlated with the PV concentration in the standard or spiked sample. The limit of detection for qualitative (LOD) and quantitative detection (LOQ) were 2 ng/mL and 0.691 ng/mL, respectively. Besides, the detection time of this ICA strip was within 15 min. The recovery rates ranged from 104.0% to 117.4%, within an acceptable level (80–120%). Moreover, the developed assay also showed high cross reaction in different fish species. These results demonstrated that the established test strip has the potential to be used as a rapid screening tool for large scale determination of PV in foodstuffs.
研究领域:
免疫学
·
食品科学
·
纳米技术
·
过敏学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESS ENGINEERING
·
2026
·Vol. 49
·期 2
·e70338
·
IF 3.5
Yingjian Liu,Chenhong Zhong,Haoxuan Li,Dingjin Li,Chunli Deng,Yipeng Gu,Mubo Song
Mango, as a highly perishable climacteric fruit, incurs significant postharvest losses due to rapid ripening and spoilage. To address this challenge, environmentally friendly bioactive coating technology has emerged as a promising preservation strategy. This study developed a novel coating based on fish gelatin (FG), enhanced by a metal-phenolic network (MPN) formed through the coordination between tannic acid (TA) and Fe 3+ ions. By systematically optimizing the molar ratio of TA to Fe 3+ , the formulation with a 0.2% Fe 3+ concentration (FG/MPN 2 ) was identified as optimal, exhibiting the best system stability (zeta potential: +16.32 ± 1.21 mV) and micro-uniformity (PDI < 0.3). Structural analyses via Fourier-transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy confirmed the formation of a stable composite structure within the FG matrix. Benefiting from this, the FG/MPN 2 coating demonstrated significantly enhanced barrier properties, interfacial adhesion, and excellent antioxidant activity. Finally, the coating was applied to mango preservation (25°C, 60% RH). The preservation results indicated that the coating could significantly inhibit weight loss, maintain higher fruit firmness (17.92 ± 0.50 N) on the 12th day, effectively delay color yellowing, and reduce lipid peroxidation damage, thereby extending the shelf life.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Zhang Mei,Yu Bingzhi,Wang Qiuyu,Wang Jing,Shao Luze,Sun Qinqin,Zhou Jingqing,Chen Shuxin,Lu Xinyang,Yang Kun,Hou Jie,Lin Daohui
Household cooking reshapes antibiotic exposure from fish
not merely
by reducing parent residues, but by redistributing and transforming
mixtures ultimately consumed. Here, 60 antibiotics were surveyed in
10 commercial aquatic products, and snakehead fish ( Channa argus ) was selected as a model to compare
boiling, steaming, and air frying. All treatments reduced parent antibiotic
residues in muscle, with boiling yielding the lowest retention (18.42
± 0.41%). During boiling, muscle-associated parent antibiotics
decreased by 83.38 ± 1.04%, but only 13.70 ± 2.53% of this
loss was recovered in broth. Boiling also caused the greatest loss
of immobilized water and tissue fragmentation, while moist heating
generated a tightly associated water fraction during tissue restructuring.
Nontarget screening tentatively assigned 15 enrofloxacin transformation
products and revealed treatment-dependent profiles and prediction-weighted
signals. These findings indicate that parent-compound depletion alone
may overlook cooking-induced partitioning, broth coconsumption, and
putative transformation products.
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 10
·1818
·
IF 5.1
Mingjing Zheng,Yiman Wei,Jinling Hong,Zhipeng Li,Yanbing Zhu,Tao Hong,Zedong Jiang,Hui Ni
The effects of pomelo peel-derived dietary fibers (total dietary fiber, cellulose, and microcrystalline cellulose) onin vitrosimulated gastrointestinal digestion and fermentation characteristics of silver carp fish balls were systematically investigated. Our findings revealed that pomelo peel dietary fibers significantly enhanced protein digestibility (highest increased by 18.58%), free amino acid content (most elevated by 13.27%), and slow digestion starch content (highest increased by 64.97%) in fish balls, suggesting an improved nutritional quality of fish balls. Moreover, pomelo peel-derived dietary fibers increased the content of short-chain fatty acids in the digestive fish balls at the late stage of fermentation (48 h) and caused changes in gut microbiota with reducing the ratio ofFirmicutestoBacteroidetes(F/B), the abundance ofEscherichia-ShigellaandStreptococcus, and increasing the levels of probioticsBacteroidesandPhascolarctobacterium. These suggested that pomelo peel-derived dietary fibers could promote the digestive characteristics of fish balls, effectively exerting prebiotic effects by regulating gut microbiota. The results could provide a scientific basis for the enhanced modification of intestinal digestion and fermentation of fish balls with dietary fibers.
研究领域:
食品科学
·
营养学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Qingchun Zhang,Hao Zhu,Rui Wang,Jianfei He,Christos Ritzoulis,Owen Griffith Jones,Weilin Liu,Wei Tang,Jianhua Liu
Effect of prior hydrolysis and subsequent 36 h glycation on the ability of amyloid-like fibrils derived from β-Lactoglobulin (FG 36 Hβ-Lg) to provide physicochemical stability to fish oil emulsions was systematically determined under varying pH, ionic strength, thermal, and storage conditions. In vitro digestion studies coupled with a Caco-2 cell model investigation evaluated the emulsions' cellular uptake, transmembrane transport, and antioxidant properties. GC–MS analysis of the post-digestion micellar phase revealed that emulsification enhanced the apparent permeability of eicosapentaenoic acid (EPA). FG 36 Hβ-Lg conferred superior EPA transport efficiency and the strongest cellular antioxidant effects on emulsions, compared to proteins modified by hydrolysis or glycation alone, or lacking fibrillar structure. Parallel reaction monitoring showed that FG 36 Hβ-Lg emulsions upregulated key regulatory proteins involved in lipid metabolism and inflammation, such as HMGCR and NFKB1. These findings demonstrate that modified β-Lactoglobulin fibrils improved physical and oxidative stability of emulsions and show promise to enhance bioavailability of omega-3 fatty acids.
研究领域:
食品科学与技术
·
营养生物化学
·
胶体与界面科学
·
分子营养学
·
生物物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED ENGINEERING MATERIALS
·
2020
·Vol. 23
·期 1
·2000821
·
IF 3.217
Wanting Rong,Haifeng Zhang,Tengjiao Zhang,Zhigang Mao,Xiaowei Liu,Keguan Song
Inspired by the bionic fish scale surfaces containing micro/nanostructured arrays, herein, the applications of lubricant-impregnated anisotropic slippery surfaces (LIASSs) using laser ablation of aluminum–magnesium alloys are proposed. Different hydrophobic properties are presented on the LIASSs along the parallel direction and the reverse direction defined as directions A and B of bionic fish scale micro/nanostructures. A self-assembled solid–liquid interface friction test device is set up to demonstrate the drag reduction property of LIASSs. The drag reduction ratios are found to be 51.09% and 44.88% along directions A and B, respectively. With the increase in the velocity, the drag reduction ratios of LIASSs can also be kept near 50%. Simulation models are established to study the drag reduction mechanism of LIASSs in laminar flows. Liquid–liquid repellency has a lubricating effect that can increase mobility and reduce viscous resistance. In this way, it improves the fluidity of the liquid and reduces drag. The drag reduction ratio in direction A is superior to that in direction B for the same flow velocity. The results of the simulation are consistent with the experiments results. LIASSs represents an effective strategy to drag reduction and reducing energy consumption in liquid directional transport and marine vessels.
研究领域:
材料科学
·
仿生学
·
流体力学
✓ 已验证
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 9
·1757
·
IF 5.2
Yuhan Chen,Hanzhi Jing,Shanbai Xiong,Anne Manyande,Hongying Du
Pork and grass carp are commonly consumed animal protein sources, classified as red meat and white meat, respectively. This study aimed to better understand the differences in digestive behavior, nutrition, and functionality during digestion between these two types of meat after fat removal. The results showed that grass carp was more easily digested than pork, with a higher degree of hydrolysis, a smaller protein particle size, and a greater release of oligopeptides and amino acids (p< 0.05). During gastric digestion, all α-helix structures were destroyed, and the effect of the whole digestion process on the secondary and tertiary structure of pork protein was greater than that of grass carp. The antioxidant properties of the digestive fluids from the two types of meat showed different strengths in various assays, but the correlation analysis revealed that TCA-soluble peptides, random coil content, and particle size significantly influenced both types of meat. These findings provide new insights into the structural state and antioxidant properties of protein in meat digestion, which contribute to our understanding of the nutritional value of pork and grass carp.Keywords:pork;grass carp;digestion;hydrolysis;antioxidant properties;protein structure
研究领域:
食品科学
·
营养学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2018
·
IF 2.441
Tang Tang,Fangdi Wei,Xu Wang,Yujie Ma,Yueyue Song,Yunsu Ma,Quan Song,Guanhong Xu,Yao Cen,Qin Hu
A novel molecularly imprinted stir bar (MI-SB) for sorptive extraction of semicarbazide (SEM) was prepared in present paper. The coating of the stir bar was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, dynamic adsorption and static adsorption tests. The saturated adsorption of MI-SB was about 4 times over that of non-imprinted stir bar (NI-SB). The selectivity of MI-SB for SEM was much better than NI-SB. A method to determine SEM was established by coupling MI-SB sorptive extraction with HPLC-UV. The liner range was 1–100 ng/mL for SEM with a correlation coefficient of 0.9985. The limit of detection was about 0.59 ng/mL, which was below the minimum required performance limit of SEM in meat products regulated by European Union. The method was applied to the determination of SEM in fish samples with satisfactory results.
研究领域:
分析化学
·
食品安全
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Yunchuan Lei,Yubo Yin,Xinyuan Qiao,Jinming Gao,Xiaoyang Wei,Yuanyuan Shao,Dongbing Li,Jesse Zhu
This study pioneers a novel strategy to transform passive polysaccharide walls into bioactive delivery systems by integrating hyaluronic acid (HA, 300 kDa) and fish collagen peptides (CP) into polysaccharide matrices (β-cyclodextrin [CD], chitosan [CT], maltodextrin [MD]). Optimization of HA-CP ratios (1:1 w /w) and spray drying parameters enhanced key metrics: MD-based powders achieved a 147% increase in oil holdup (OH, 0.047 to 0.116 mL g −1 ) and 105% higher encapsulation efficiency (EE, 38.7% to 79.4%). CT-HA-CP composites exhibited peak EE (83.2%), while CD-HA-CP improved OH by 70% (0.122 to 0.208 mL g −1 ). HA-CP synergy reduced oil-water contact angles, ensured rapid dissolution, and produced smooth spherical particles ( D 50 10–20 μm). This work demonstrates that HA and CP can be physically incorporated into oil powders without chemical reactions, transforming passive wall materials into bioactive carriers and providing a physicochemical foundation for functional applications leveraging their known bioactivities.
✓ 已验证
DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2026
·
IF 4.6
Ling Zhang,Zi-Shang Liu,Si-Si Xiong,Yan-Zou Dong,Yue-Yun Guo,Ding-Dong Zhang,Guang-Zhen Jiang,Wen-Bin Liu,Xiang-Fei Li
The widespread use of magnesium oxide nanoparticles (MgO NPs) presents a potential biological effect and toxicity to both aquatic ecosystems and human well-being. However, the potential toxicity of MgO NPs has been barely investigated. In this study, the toxicological evaluation of MgO NPs was systematically determined by using fish Megalobrama amblycephala as a model in both in vivo and in vitro studies. In vivo, fish were exposed to three concentrations of MgO NPs in water (0, 50 and 100 mg/L). In vitro, primary hepatocytes were exposed to three concentrations (0, 16 and 32 mM) of MgO NPs in the medium. Our results demonstrated that MgO NPs impaired Mg homeostasis leading to mitochondrial dysfunction, and triggered apoptosis in both in vivo and in vitro. This was evidenced by the elevated mitochondrial Mg content and Trpm7 and Mrs2 protein expression, a loss of MMP, the impaired activities of mitochondrial complexes IV and V, an upregulation of Cyt-c and caspase 3, and a downregulation of Bcl-2. Overall, MgO NP exposure disrupts the Mg homeostasis, thereby leading to mitochondrial dysfunction and apoptosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yi He,Rongtai Wan,Gen Li,Baoyang Lu
Organohydrogel-based plant strain sensors hold significant potential for enabling accurate and real-time monitoring of plant growth processes. However, existing strain sensors typically face challenges such as inferior biocompatibility, trade-off between sensing performance and mechanical properties, as well as poor long-term stability, leading to inaccurate monitoring and plant tissue damage and thus hindering their practical applications. Herein, we propose a synergistic metal ion and multiple hydrogen bond dual crosslinking strategy to develop a biomass-based fish gelatin organohydrogel as a strain sensing material. The resultant organohydrogel simultaneously exhibits excellent mechanical properties (Young's modulus of 99.9 kPa and strong adhesiveness of 60 kPa), high sensing performance (GF = 2.13, stable response across a wide temperature range from −80 °C to 25 °C), outstanding plant tissue and human cell biocompatibility, and long-term stability (over 5000 loading-unloading cycles under 100% strain), demonstrating superior overall performance to most existing organohydrogels. To harness these unique material performances, we fabricate a sandwich-structured plant strain sensor for long-term monitoring of plant growth. The fabricated strain sensor enables successful real-time monitoring of the growth dynamics of lotus stems and pomelo fruits with high accuracy and long-term stability up to three weeks. Our novel design strategy of high-performance organohydrogels enables high-fidelity plant growth monitoring, unlocking new potentials for advancing data-driven smart and precision farming practices.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Jin-Ye Lu,Qiu-Yun Chen,Su-Ci Meng,Chang-Jian Feng
Phthalates can penetrate the environment and enrich various aquatic organisms through the food chain, which is involved in promoting the growth of breast cancer. It is of current interest to develop new sensors for phthalates. We herein reported a hydrogen-bond competing fluorescent sensor, BANP, for the detection of dibutyl phthalate (DBP). The BANP compound was synthesized by assembling andrographolide (Andro), nitro- and cyano-substituted BODIPY dye (BCN), and polyethylene glycol derivatives (DSPE-mPEG5000). BANP was found to be a turn-on fluorescent probe for DBP in water with a detection limit of 0.13 μg/g; the DBP-water system acts as a hydrogen bond switch to turn on the fluorescence. And BANP fluorescently detected DBP in contaminated fish meat. Moreover, BANP sensed the DBP-induced growth of human breast cancer MCF-7 cells, and the release of Andro in the DBP-cultivated cancer cells inhibited the proliferation of the MCF-7 cells. Taken together, BANP is a DBP-responsive probe for sensitive DBP detection in water, cells, and fish meats. The BANP sensor may be used in both in vitro fluorescence and cellular imaging analyses. Our results show that guest-induced reassembly brings forth significant fluorescence change, which is a promising way of designing new fluorescent probes for the analysis of phthalates in the environment and food.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）