ENZYME AND MICROBIAL TECHNOLOGY
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2019
·
IF 3.553
Yuping Shen,Man Wang,Jinwei Zhou,Yufei Chen,Lili Xu,Mengru Wu,Guohua Xia,James P. Tam,Jiangnan Yu,Xiying Teng,Huan Yang,Xiaobin Jia
Baohuoside I, a rare secondary glycoside from Epimedii Folium, has anti-osteoporosis and other pharmacological effects. In addition, it exhibits improved bioactivities compared with its original glycoside icariin. Conventional methods for the production of baohuoside I, such as acid hydrolysis, exhibit very low efficiency with serious pollution and substantial byproducts. The aim of this study was to develop an eco-efficient biphasic enzymatic hydrolysis system for the green production of rare baohuoside I. β -glucanase was selected to hydrolyze icariin and showed better performance than β -glucosidase. The biphasic system was constructed using HAc-NaAc buffer (pH 4.5) containing β -glucanase/icariin (C icariin = 100 mg/mL; 1:1, w/w ) and propyl acetate (1:2, v/v ), and the hydrolysis was performed at 60℃ for 6 h. The hydrolysis capacity of icariin in the system was largely increased from 0.5 mg/mL to 100 mg/mL. The conversion ratio of icariin was greater than 99% in a 100-fold scale-up pilot test, demonstrating its strong potential for industrial applications. Furthermore, a process flow and production plant were designed to produce 1 ton of baohuoside I annually, and the profit would be approximately 4.5-fold its total cost. Biphasic enzymatic hydrolysis is an eco-efficient technology to produce baohuoside I and represents a promising method in the pharmaceutical industry.
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NEW JOURNAL OF CHEMISTRY
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2019
·Vol. 43
·期 34
·13408-13417
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IF 3.069
Qiong Wei,Hong-Ting Chen,Li Liu,Xin-Xin Zhong,Lei Wang,Fa-Bao Li,Heng-Jiang Cong,Wai-Yeung Wong,Khalid A. Alamry,Hai-Mei Qin
High efficiency copper(I) halide complexes containing rigid diphosphine ligands have attracted much attention. However, rigid bidentate phosphine ligands have been rarely reported so far. Here, an electron-rich heteroaryl ring grafted with two electron donating methyl groups was first included to construct a rigid bidentate phosphine ligand, three four-coordinate dinuclear copper(I) halide complexes, [CuX(dpmt)]2 (dpmt = 3,4-bis(diphenylphosphino)-2,5-dimethylthiophene, X = I (1), Br (2) and Cl (3)), were synthesized, and their molecular structures and photophysical properties were investigated. The structural analysis reveals that two copper(I) centers are bridged by two halogen ligands to form a dinuclear structure with a four-membered Cu2X2 ring. These complexes exhibit intense bluish green to yellow green emission in the solid state at room temperature and have peak emission wavelengths at 490–543 nm with microsecond lifetimes (τave = 3.9–13.6 μs, Φ = 0.26–0.35). The emission of complex 1 mainly originates from MLCT and ligand-centered transitions. Similar emissive behaviors for complexes 2 and 3 are possibly due to ligand-centered transition. The solution-processed devices of complex 3 exhibit stable yellow emission with CIE(x, y) of (0.42, 0.52) for doped and nondoped devices.
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FOOD CHEMISTRY
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2018
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IF 4.946
Diandian Deng,Hong Yang,Chang Liu,Kang Zhao,Jianguo Li,Anping Deng
An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for ultrasensitive determination of Sudan I in food samples was reported. Gold-silver core-shell bimetallic nanorods (referred to as [email protected] NRs) were synthesized, characterized and used as the substrate for preparation of the ICA. Polyclonal antibody against Sudan I was immobilized on the surface of the [email protected] NRs carrying the Raman reporter 5,5′-dithiobis (2-nitrobenzoic acid). The Raman scattering intensity on the test line was used for quantitation of Sudan I. The assay was completed in 15 min. IC 50 and limit of detection (LOD) were 30 pg mL −1 and 0.2 pg mL −1 , respectively. There was no cross-reactivity (CR) of the assay with Sunset Yellow, Lemon Yellow and Brilliant blue FCF, but only 3.53%-9.74% CR with Sudan II, III and IV. The recoveries of Sudan I from spiked food samples were in the range of 88.9–107.6% with relative standard deviation of 3.7–8.7% (n = 3).
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Biomed Research International
·
2018
·
IF 2.583
Yong He,Wei Liu,Lianxiong Guan,Jielin Chen,Li Duan,Zhaofeng Jia,Jianghong Huang,Wencui Li,Jianquan Liu,Jianyi Xiong,Lijun Liu,Daping Wang
Scaffolds play an important role in tissue engineering and their structure and biocompatibility have great influence on cell behaviors. In this study, poly(l-lactide-co-ε-caprolactone) (PLCL) scaffolds were printed by a 3D printing technology, low-temperature deposition manufacturing (LDM), and then PLCL scaffolds were treated by alkali and coated with collagen type I (COLI). The scaffolds were characterized by scanning electron microscopy (SEM), porosity test, mechanical test, and infrared spectroscopy. The prepared PLCL and PLCL-COLI scaffolds had three-dimensional (3D) porous structure and they not only have macropores but also have micropores in the deposited lines. Although the mechanical property of PLCL-COLI was slightly lower than that of PLCL scaffold, the hydrophilicity of PLCL-COLI was significantly enhanced. Rabbit articular chondrocytes were extracted and were identified as chondrocytes by toluidine blue staining. To study the biocompatibility, the chondrocytes were seeded on scaffolds for 1, 3, 5, 7, and 10 days. MTT assay showed that the proliferation of chondrocytes on PLCL-COLI scaffold was better than that on PLCL scaffold. And the morphology of cells on PLCL-COLI after 1-day culture was much better than that on PLCL. This 3D-printed PLCL scaffold coated with COLI shows a great potential application in tissue engineering.
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XENOBIOTICA
·
2018
·
IF 1.857
Yue Li,Yujuan Fan,Huizong Su,Qian Wang,Guo-Fu Li,Yiyang Hu,Jian Jiang,Bo Tan,Furong Qiu
Tanshinone I (TSI) is a lipophilic diterpene in Salvia miltiorrhiza with versatile pharmacological activities. However, metabolic pathway of TSI in human is unknown.In this study, we determined major metabolites of TSI using a preparation of human liver microsomes (HLMs) by HPLC-UV and Q-Trap mass spectrometer. A total of 6 metabolites were detected, which indicated the presence of hydroxylation, reduction as well as glucuronidation.Selective chemical inhibition and purified cytochrome P450 (CYP450) isoform screening experiments revealed that CYP2A6 was primarily responsible for TSI Phase I metabolism. Part of generated hydroxylated TSI was glucuronidated via several glucuronosyltransferase (UGT) isoforms including UGT1A1, UGT1A3, UGT1A7, UGT1A9, as well as extrahepatic expressed isoforms UGT1A8 and UGT1A10. TSI could be reduced to a relatively unstable hydroquinone intermediate by NAD(P)H: quinone oxidoreductase 1 (NQO1), and then immediately conjugated with glucuronic acid by a panel of UGTs, especially UGT1A9, UGT1A1 and UGT1A8. Additionally, NQO1 could also reduce hydroxylated TSI to a hydroquinone intermediate, which was immediately glucuronidated by UGT1A1.The study demonstrated that hydroxylation, reduction as well as glucuronidation were the major pathways for TSI biotransformation, and six metabolites generated by CYPs, NQO1 and UGTs were found in HLMs and S9 subcellular fractions.
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NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 20
·16814-16822
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IF 3.201
Wei Huang,Shucheng Liu,Jinxin Liu,Wenli Zhang,Jianming Pan
A facile strategy to fabricate crown ether (2-methylol-12-crown-4, 2M12C4) immobilized porous polymers (PGMA-CE) was reported toward lithium(I) (Li+) recovery. Macroporous polymer foam (polymeric glycidyl methacrylate, PGMA) with abundant epoxy groups was firstly prepared as a support using a high internal phase emulsion (HIPE) template. And then 2M12C4 was covalently attached onto the surface of PGMA to endow the PGMA-CE with good stability and high selectivity. PGMA-CE was characterized by SEM, FT-IR, XPS, and 13C NMR spectroscopy, and its porous structure and the successful introduction of 2M12C4 were confirmed. In batch mode experiments under optimum conditions, PGMA-CE exhibited fast adsorption kinetics (the equilibrium time was 3.0 h), and the kinetics data were well described using a pseudo-second-order model. A Langmuir-type monolayer adsorption was investigated, and the calculated maximum equilibrium adsorption capacity was 3.15 mg g−1. In addition, PGMA-CE displayed excellent selectivity for Li+ in the presence of multiple interfering ions (Na+, K+, Mg+, and Ca2+), and the selective separation factors (α) were above 4.75. Regeneration analysis showed that the adsorption capacity of PGMA-CE toward Li+ after five cycles was 91.8% of the first cycle.
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DALTON TRANSACTIONS
·
2018
·Vol. 47
·期 29
·9845-9849
·
IF 4.099
Daqin Chen,Xiao Chen,Junni Li,Xinyue Li,Jiasong Zhong
The preparation of CsPbX3 nanoplatelets and the investigation of their optical properties have been rarely reported. Herein, the successful development of all-inorganic CsPbX3 (X = Cl, Br, or I) perovskite quantum dots has been achieved and their unique optical and electrical properties are discussed. Apre-dissolution assisted solvothermal reaction was developed to synthesize ultrathin (∼3 nm) CsPbBr3 nanoplatelets with strong exciton absorption, with a 0.41 eV blue-shifted emission relative to that of CsPbBr3 nanocrystals and a high quantum yield of 28%. Anion exchange allowed the variation of the halogen content of the nanoplatelets and allowed them to retain their shape and size and achieve multi-color emission.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2019
·Vol. 30
·期 16
·14977-14986
·
IF 2.195
Tang Hongbo,Li Qiuyue,Zhou Jian
The effect of low I/Ag ratios on the performance of silver thick films containing micro-sized silver particles treated with surface iodination has been investigated. The film with I/Ag ratio of 1:100 exhibited high electrical conductivity and densification, while that of 2:100 presented better adhesion. The conductivity and densification of the film reached the highest at 550 °C. Optimal conductivity of the film with I/Ag ratio of 1:100 and 2:100 was 11.45 and 15.85 mΩ/sq., respectively. In addition, the film adhesion was enhanced by heating. Different trends observed in electrical conductivity and adhesion as functions of temperature is primarily attributed to shrinkage of modified particles. This work suggested that the I/Ag ratio plays a vital effect on the performance of the silver thick film.
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SENSORS AND ACTUATORS B-CHEMICAL
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2017
·
IF 5.401
Haiyan Liu,Yunfeng Bai,Jun Qin,Haiyan Wang,Yuzhen Wang,Zezhong Chen,Feng Feng
A novel sensitive fluorescence method was developed for adenosine detection by using adenosine aptamer, Exo I and a 2-aminopurine-modified DNA (2-AP probe). 2-AP probe was completely complementary to adenosine aptamer. This strategy took advantage of the high binding affinity of adenosine aptamer and the susceptibility of 2-aminopurine (2-AP) to the local base stacking environment. In the absence of the adenosine, the formation of double-stranded DNA (dsDNA) through the hybridization of the aptamer with the 2-AP probe prevented the digestion of 2-AP probe by Exo I. The fluorescence of 2-AP was significantly quenched due to the base-stacking interaction. In the presence of adenosine, the binding of adenosine with the aptamer prevented the hybridization between aptamer and 2-AP probe, leading to the digestion of 2-AP probe by Exo I and the release of 2-AP. The fluorescence was recovered. A limit of detection of 0.30 μM and a linear range from 10 μM to 600 μM ( R 2 = 0.9933) were achieved by this assay. Moreover, adenosine analogs did not produce any significant change in the fluorescence intensity response, indicating good selectivity of this sensor for adenosine detection. Finally, it was used for the diluted serum sample.
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LANGMUIR
·
2023
·
IF 3.9
Daiyun Xu,Xu Chen,Yongxiao Li,Zhidong Chen,Wanting Xu,Xinpei Wang,Yonghui Lv,Zhe Wang,Meiying Wu,Gang Liu,Junqing Wang
Nanodisc (ND)-forming membrane scaffold proteins or peptides developed from apolipoprotein A-I (apoA-I) have led to considerable promise in structural biology and therapeutic applications. However, the rationale and regularity characteristics in peptide sequence design remain inconclusive. Here, we proposed a consensus-based normalization approach through the reversed engineering of apoA-IΔ1–45 to design reconfigurable apoA-I peptide analogs (APAs) for tunable ND assembly. We present extensive morphological validations and computational simulation analyses on divergent APA-NDs that are generated by our method. Fifteen divergent APAs were generated accordingly to study the assembly machinery of NDs. We show that APA designs exhibit multifactorial influence in terms of varying APA tandem repeats, sequence composition, and lipid-to-APA ratio to form tunable diameters of NDs. There is a strong positive correlation between DMPC-to-APA ratios and ND diameters. Longer APA with more tandem repeats tends to yield higher particle size homogeneity. Our results also suggest proline is a dispensable residue for the APA-ND formation. Interestingly, proline-rich substitution not only provides an inward-bending effect in forming smaller NDs but also induces the cumulative chain flexibility that enables larger ND formation at higher lipid ratios. Additionally, proline-tryptophan residues in APAs play a dominant role in forming larger NDs. Molecular simulation shows that enriched basic and acidic residues in APAs evoke abundant hydrogen-bond and salt bridge networks to reinforce the structural stability of APA-NDs. Together, our findings provide a rational basis for understanding APA design. The proposed model could be extended to other apolipoproteins for desired ND engineering.
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INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Dayu Huang,Qiuyun Ouyang,Jinjiang Wu,Youchao Kong,Bo Wang,Hongzhou Lian,Jun Lin
Broadband emission with a large Stokes shift is important to obtain an excellent color rendering index of the solid-state lighting device. Among low-dimensional material and perovskite-like phosphors with broadband self-trapped emission, Sn-based phosphors have attracted much attention due to their high photoluminescence quantum yield (PLQY). However, the disadvantage is that the synthesis of Sn-based phosphors needs to be performed in a glovebox. Upon photoexcitation, the broadband emission of self-trapped excitons results from exciton–phonon coupling induced by the transient distortion of the lattice. Low-dimensional material structures often promote self-trapped emission because of more vibrational degrees of freedom and easier polarization under photoexcitation. Here, zero-dimensional (0D) SnX2 (X = Br, I) single crystals are synthesized by the solvent evaporation method in the air. SnX2 emits blue light, broadband yellow light, and deep red light, among which SnBr2 has the best luminescence performance. The photoluminescence quantum yield (PLQY) of SnBr2 reaches 85% and the Stokes shift reaches 265 nm. The PL intensity of SnX2 is linearly related to excitation power, which preliminarily indicates that the origin of SnX2 luminescence is attributed to self-trapped emission (STE). The white light-emitting diodes (WLEDs) were fabricated using yellow-emitting SnBr2 and blue-emitting BaMgAl10O17:Eu2+, which has a low correlated color temperature (3160 K) and a relatively common color rendering index (79).
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Journal of Energetic Materials
·
2022
·
IF 2.421
Xiaopeng Zhang,Yige Wu,Pengcheng Wang,Qiuhan Lin,Shusen Chen,Shaohua Jin,Yuangang Xu,Ming Lu
As a new structural motif, an interesting 3D energetic metal – framework of silver ions and 3,4-diaminofurazan, [Ag+(daf)NO3−]n, is successfully synthesized and the structure is determined by single-crystal X-ray diffraction. The effects of hydrogen bond, coordination bond, adsorption and filling interactions make this compound have a high density (2.694 g·cm−3 at 293 K). The calculated detonation velocity and the sensitivities are all comparable with trinitrotoluene (TNT). The onset decomposition temperature is equal to hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), state-of-the-art explosive. This framework provides a potential superiority of E-MOFs for energetic materials.
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