JOURNAL OF APPLIED MICROBIOLOGY
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2017
·Vol. 124
·期 2
·469-479
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IF 2.099
T. Cheng,X. Xu,W. Zhang,L. Chen,T. Liu
Aims To establish a proper protoplast-preparation route from enriched motile flagellates and nonmotile resting cells of Haematococcus pluvialis . Methods and Results Through cultivations in two mixotrophic media, enriched Haematococcus flagellates and resting cells were respectively produced and applied in enzymatic protoplast preparations. Great differences of enzymatic sensitivity and osmotic-lability were identified between them. Flagellates showed the same osmotic-lability as protoplasts and the extracellular matrix-removing rate was applied for an evaluation of protoplast-releasing. During the treatment of flagellates, an addition of more than 0·2 mmol l −1 Ca 2+ was found to be essential for maintenance of high cellular viability. More than 80% cellular viability and a 90% protoplast-releasing rate were obtained simultaneously after 2–3 h treatment of 0·06% proteinase K in 0·05 mol l −1 Tris-HCl (pH7·8) buffer with 0·2 mmol l −1 CaCl 2 and 0·2 mol l −1 sorbitol/mannitol. For resting cells, a treatment of both 0·12% proteinase K and a combination of 2% cellulase + 1% snailase could function similarly in order to degrade the cellulosic cell wall, while the protoplast yield was limited to about 40%, due to the existence of an undegradable secondary wall in the mature resting cell. Conclusion Proteinase K was efficient for protoplast-releasing from either flagellates or resting cells. Due to the great difference of enzymatic sensitivity and osmotic-lability between flagellates and resting cells, it was necessary to select a different enzymatic treating process based upon the main cell type in the culture. A better protoplast-preparing efficiency was obtained from Haematococcus cells when flagellates were in the majority. Significance and Impact of the Study The protoplast preparation of H. pluvialis was firstly established based on two main cell types of H. pluvialis , motile flagellates and nonmotile resting cells. Increase of flagellate stability and viable protoplast-preparing efficiency through addition of Ca 2+ in enzymatic solution was firstly reported.
研究领域:
微生物学
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生物技术
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植物科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Jie Zhou,Hao Liu,Chao Xiong,Peng Hu,Hao Wang,Xuyu Wang,Hongbing Ji
Propane dehydrogenation (PDH) has been a promising method to satisfy the increasing demand for propylene, but Pt-based catalysts always suffer from sintering and coke formation. Developing stable and efficient catalysts for PDH is of great significance for the industry. Herein, the paper reports that a catalyst with potassium-promoted Pt-In bimetallic clusters encapsulated in silicalite-1 (S-1) zeolite works as an efficient and stable catalyst for PDH. The catalyst achieves a propane conversion of 43.73 % with 97.14 % selectivity of propene and an extremely high propylene productivity of 20.33 mol g Pt -1 h -1 with a weight hourly space velocity (WHSV) of 6.75 h −1 . No obvious deactivation occurs after 48 h on stream, affording an extremely low deactivation constant of 0.0025 h −1 . The introduction of indium species greatly improves the propene selectivity and restricts the coke formation. The introduction of K + into S-1 can significantly enhance the catalytic activity and the stability of the catalyst. The catalyst with excellent activity and stability is potential for industrial application.
研究领域:
催化
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化学工程
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材料科学
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产品关联
DOI
高影响因子
POLYMER
·
2025
·
IF 4.5
Jinqi Ye,Nasha Zeng,Lili Wang,Keying Zheng,Junyi Lu,Minghua Wu
Disperse dyes wash-free inkjet-dyeing is one of the important research and development directions to achieve clean dyeing of polyester fabrics. Wash-free binders are needed to be designed with a special molecular structure to achieve good colour yield and fixation during dyeing. Specifically, isophorone diisocyanate and polypropylene glycol were used as the monomers of the hard and soft chain segments of the polymer, respectively, and KH-560 as the capping agent, to prepare a series of end-group cross-linked organosilicon modified waterborne polyurethane (SWPU). It was applied as wash-free binder for the digital inkjet-dyed polyester fabrics. The results showed that, for 2 wt% KH-560 and 40 wt% hard chain segments, the SWPU film had good water resistance, mechanical properties, and adhesion properties, and the best dispersed dye directional effect. With the addition of SWPU in the dye ink of 8 wt%, the front K/S value of the SWPU wash-free digital inkjet-dyes fabric reached 16.86, and the dry/wet rubbing fastness achieved grades 4–5 and 3–4, respectively. Compared with the pure dye inkjet-dyed fabric, its front K/S value increased by 23.9 %, and its dry/wet rubbing fastness improved by grades 1 and 0.5, respectively.
研究领域:
高分子科学
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纺织化学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
BIOSCIENCE REPORTS
·
2020
·Vol. 40
·期 7
·
IF 2.942
Huang Ming-Zhi,Zhuang Yong,Ning Xu,Zhang Hao,Shen Zhi-Min,Shang Xian-Wen
Osteoporosis is a metabolic bone disease that is characterized by decreased bone density and strength due to excessive loss of bone protein and mineral content, which can be induced by increased osteoclast activity. Developing agents targeting osteoclast activation is considered to be the most effective method to reverse bone destruction and alleviate the pain caused by osteoporosis. MTT assay was conducted to detect the cell viability after artesunate treatment of RAW264.7 cells. TRACP staining and pit formation assays were performed to examine the TRACP-positive cells and pit-forming activity of osteoclasts. qRT-PCR and Western blot analysis were performed to assess the mRNA and protein expression levels of the osteoclastogenesis-related genes NFATc1, TRAP, and cathepsin k. The protein levels of RANK, p-Akt, p-p38, and p-ERK were examined by Western blotting. Luciferase reporter assay was conducted to determine whether miR-503 targeted RANK directly. Artesunate inhibited TRACP-positive cells and the pit-forming activity of osteoclasts. However, artesunate increased the expression of miR-503. Artesunate suppressed osteoclastogenesis-related gene expression and RANKL-induced activation of MAPKs and the AKT pathway. In addition, miR-503 inhibited RANK expression by directly targeting RANK during osteoclast differentiation. Artesunate inhibited osteoclastogenesis and osteoclast functions in vitro by regulating the miR-503/RANK axis and suppressing the MAPK and AKT pathways, which resulted in decreased expression of osteoclastogenesis-related markers.
研究领域:
药理学
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骨骼生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2023
·Vol. 35
·期 19
·2210637
·
IF 29.4
Yingying Zhou,Xianfeng Ping,Yusi Guo,Boon Chin Heng,Yijun Wang,Yanze Meng,Shengjie Jiang,Yan Wei,Binbin Lai,Xuehui Zhang,Xuliang Deng
Current functional assessment of biomaterial-induced stem cell lineage fate in vitro mainly relies on biomarker-dependent methods with limited accuracy and efficiency. Here a “Mesenchymal stem cell Differentiation Prediction (MeD-P)” framework for biomaterial-induced cell lineage fate prediction is reported. MeD-P contains a cell-type-specific gene expression profile as a reference by integrating public RNA-seq data related to tri-lineage differentiation (osteogenesis, chondrogenesis, and adipogenesis) of human mesenchymal stem cells (hMSCs) and a predictive model for classifying hMSCs differentiation lineages using the k-nearest neighbors (kNN) strategy. It is shown that MeD-P exhibits an overall accuracy of 90.63% on testing datasets, which is significantly higher than the model constructed based on canonical marker genes (80.21%). Moreover, evaluations of multiple biomaterials show that MeD-P provides accurate prediction of lineage fate on different types of biomaterials as early as the first week of hMSCs culture. In summary, it is demonstrated that MeD-P is an efficient and accurate strategy for stem cell lineage fate prediction and preliminary biomaterial functional evaluation.
研究领域:
干细胞生物学
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生物医学工程
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机器学习
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人工智能
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生物材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY OF MATERIALS
·
2025
·
IF 7
Yi Yi,Pengxiang Zhang,Yixin Shen,Junhui Tan,Bao-Wen Li
Solution-processable high-k perovskite nanosheets show considerable potential for applications in printed electronics due to their unique structural properties and exceptional physical characteristics. However, their dispersibility and compatibility in various organic solvents remain insufficiently understood, with a lack of systematic investigation, which restricts their broader application potential. In this study, the dispersion behavior of high-k perovskite nanosheets, specifically Ca2Nb3O10 (CNO) and Ca2NaNb4O13 (CNNO), was examined in 20 different organic solvents. By correlating the physicochemical properties of these solvents, including surface tension, dielectric constant, and empirical solvent parameters, with Hildebrand and Hansen solubility models, we identified the underlying solvent–nanosheet interaction mechanisms responsible for dispersion stability. Polar protic solvents, such as water and methanol (MeOH), as well as polar aprotic solvents, like dimethyl sulfoxide (DMSO), acetonitrile (ACN), N,N-dimethylformamide (DMF), propylene carbonate (PC), and N-methyl-2-pyrrolidone (NMP), facilitate uniform and stable nanosheet dispersions. These solvents promote dispersion stability through strong dipole–dipole interactions and moderate surface tension matching. Based on these findings, we developed a series of organic solvent-based inks incorporating high-k perovskite nanosheets, demonstrating excellent printing adaptability for electrical device fabrication, and achieving stable device performance. This work provides a comprehensive library of organic solvents that effectively disperse high-k perovskite nanosheets, thereby broadening their potential applications in printed electronic devices.
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产品关联
DOI
高影响因子
Inorganic Chemistry Frontiers
·
2019
·Vol. 6
·期 8
·2118-2125
·
IF 5.934
Xianglong Huang,Wei Wang,Jianhua Deng,Wei Gao,Dingyu Liu,Qianru Ma,Maowen Xu
In this study, a novel hollow carbon matrix was designed and prepared using a facile NaCl crystal template for the effective encapsulation of Se. This superior carbon electrode plays a significant role in facilitating sufficient energy storage and conversion of Se-based cathode materials. A large specific surface area of 416 m2 g−1 was obtained, and the matrix provided numerous hollow spheres for the storage of Se. The thermogravimetric analysis and electrochemical results show that the as-prepared Se-hollow porous carbon composite with an ideal Se content of 42% can make excellent capacity contribution. The hollow porous structure not only effectively accommodates the volumetric expansion during the discharge–charge process but also makes the composite have a good contact with the electrolyte. In addition, the 3D open framework structure can be conducive to fast electron transportation. As a result, the Se-hollow porous carbon composite exhibited outstanding electrochemical performance when evaluated as a cathode material for rechargeable K–Se batteries.
研究领域:
材料科学
·
能源存储与转换
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Jing-Yi Cao,Ze-Dong Yu,Guang Yang,Xue-Can Wang,Lu Jiang,Xi Wang,Wen-Xuan Zhao,Shu-Hai Chen,Carl Redshaw,Chuan-Zeng Wang,Takehiko Yamato
In recent years, fluorescence sensors have been widely used in explosive detection due to their portability and high efficiency. In the present work, two K-region tetrasubstituted deep-blue pyrene-based luminogens were designed and synthesized by introducing electron-donating groups. The optical properties were investigated by experimental and density functional theory (DFT) methods. Reasonable luminescence efficiency and electron rich characteristic allowed for the possible detection of nitro-explosives. Both luminogens, especially TFPy , exhibit a sensitive response towards nitroanilines ( NA ), with a limit of detection (LOD) for p -NA as low as 2.75 × 10 –8 M, which is mainly attributed to the electron-donating substituents on the periphery of the pyrene core and the extended π-conjugated structure. These research results establish a low-cost and simple strategy for the detection of trace nitroaniline.
研究领域:
化学传感器
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材料科学
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环境监测
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DOI
高影响因子
JOURNAL OF SOLUTION CHEMISTRY
·
2018
·Vol. 48
·期 3
·367-378
·
IF 1.401
Li Hongxia,Guo Lijiang,Li Jianqiang
The water activities of the MgCl 2 –RbCl–H 2 O ternary system and its sub-binary systems have been measured with the isopiestic method at 323.15 K. The measured water activities for the MgCl 2 –H 2 O and RbCl–H 2 O sub-binary systems agree well with the results reported in literature. The measured equal water activity lines of the MgCl 2 –RbCl–H 2 O ternary system are not straight lines, which means the mixing behavior of the solutions do not obey Zdanovskii’s rule. The solubility of the MgCl 2 –RbCl–H 2 O ternary system has been determined with the Flow-Cloud-Point method at 323.15 K, and the measured results are roughly consistent with values reported in the literature. The Pitzer model was selected to correlate the water activity and solubility data measured in this work. The predicted water activities and solubility isotherm for the MgCl 2 –RbCl–H 2 O ternary system at 323.15 K, with binary solution parameters only, both deviated from the experiment values. The ternary solution Pitzer model parameters were obtained by fitting the measured water activities in the ternary system. The water activities and solubility isotherms of the MgCl 2 –RbCl–H 2 O ternary system at 323.15 K were calculated including both binary and ternary solution parameters. The calculated water activities are consistent with the experimental values. The calculated solubility isotherms are roughly consistent with experiment values, except they deviate somewhat in the RbCl crystallization region.
研究领域:
化学
·
物理化学
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2016
·
IF 1.835
Hongjie Xu,Zuoxiang Zeng,Weilan Xue,Kun Li
The solubility of ethyl p-aminobenzoate (EPAB) was measured in six different alcohols (methanol, ethanol, n-propanol, n-butanol, isobutyl alcohol, isoamyl alcohol) within (283.15 to 327.15) K by the last solid disappearance method. The solubility increases with increasing temperature. The decreasing order satisfies the following sequence: methanol > ethanol > n-propanol > n-butanol > isoamyl alcohol > isobutyl alcohol. Four models, including modified Apelblat equation, λh model, NRTL model, and Wilson model, were used to correlate the experimental data of EPAB. It is found that the modified Apelblat equation, NRTL model, and Wilson model were suitable to correlate the solubility of EPAB in the selected solvents with an overall RAD less than 2%. In addition, the changes of thermodynamic properties of solution [standard molar enthalpy (ΔdisHo), standard molar entropy (ΔdisSo), and standard molar Gibbs energy (ΔdisGo)] were obtained. The results indicate that the dissolution process of EPAB in these alcohols is endothermic and an entropy-driven process.
研究领域:
物理化学
·
药学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Chunhui Wang,Haozhe Qin,Liang Cao,Dong Wang,Jiafeng Zhang,Bao Zhang,Xing Ou
Metal selenides possess tremendous potentials due to their high specific capacity for potassium storage, however the sluggish K + diffusion and polyselenide dissolution always result in inferior electrochemical performance. Herein, metal selenide (CoSe 2 ) nanorods with engineered exposed plane incorporated in reduced graphene oxide nanosheet (sc-CoSe 2 @rGO) are fabricated. In the crystalline interior, such single-crystal structure can provide unhindered path for the fast K + transfer. Besides, at the crystalline surface, the mainly exposed (011) facet with Co 2+ rich state can induce the catalytic function with enormous reaction sites, which effectively transform the polyselenide to selenide with an enhanced selenium utilization. As expected, the as-prepared sc-CoSe 2 @rGO delivers satisfied specific capacity, prominent rate property, and superior cyclic stability (a reversible capacity of 157.7 mAh g −1 at high rate of 3.0 A g −1 after 600 cycles). This single-crystalline architecture design for intrinsic K-ion diffusion and surficial optimization is pioneeringly investigated, which provides a new insight into designing and fabricating metal-ion storage materials.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
Science Advances
·
2023
·Vol. 9
·期 24
·
IF 13.6
Mingrui Wang,Peng Wang,Guanghui Zhang,Zening Cheng,Mengmeng Zhang,Yulong Liu,Rongtan Li,Jie Zhu,Jianyang Wang,Kai Bian,Yi Liu,Fanshu Ding,Thomas P. Senftle,Xiaowa Nie,Qiang Fu,Chunshan Song,Xinwen Guo
The decomposition of cobalt carbide (Co 2 C) to metallic cobalt in CO 2 hydrogenation results in a notable drop in the selectivity of valued C 2+ products, and the stabilization of Co 2 C remains a grand challenge. Here, we report an in situ synthesized K-Co 2 C catalyst, and the selectivity of C 2+ hydrocarbons in CO 2 hydrogenation achieves 67.3% at 300°C, 3.0 MPa. Experimental and theoretical results elucidate that CoO transforms to Co 2 C in the reaction, while the stabilization of Co 2 C is dependent on the reaction atmosphere and the K promoter. During the carburization, the K promoter and H 2 O jointly assist in the formation of surface C * species via the carboxylate intermediate, while the adsorption of C * on CoO is enhanced by the K promoter. The lifetime of the K-Co 2 C is further prolonged from 35 hours to over 200 hours by co-feeding H 2 O. This work provides a fundamental understanding toward the role of H 2 O in Co 2 C chemistry, as well as the potential of extending its application in other reactions.
研究领域:
催化化学
·
化学工程
·
材料科学
·
可持续能源
·
多相催化
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DOI
高影响因子
危险品化学品经营许可证（带存储）