ACS Applied Bio Materials
·
2019
·
IF 0
Zaizai Dong,Chuanhao Tang,Zhen Zhang,Wei Zhou,Rong Zhao,Lina Wang,Jiachao Xu,Yayun Wu,Jiang Wu,Xing Zhang,Li Xu,Libo Zhao,Xiaohong Fang
Exosomes, which are 30–150 nm extracellular vesicles containing a variety of biomolecules (i.e., proteins, RNA, DNA, and lipids), have emerged as important analytes in liquid biopsy. Although exosome detection offers a valuable chance to understand disease status in a multidimensional manner, comprehensive analysis of different exosomal biomolecules with limited specimens remains a challenge. Herein, we introduced an aptamer-based PCR (polymerase chain reaction) platform and realized highly sensitive detection of CD63 protein positive exosomes as low as 50 particles/μL. More importantly, simultaneous analysis of exosomal RNA and surface protein is available using this method. In a proof of concept experiment, simultaneous detection of two immunotherapy-related biomarkers, programmed death-ligand 1 (PD-L1) protein and indoleamine 2,3-dioxygenase 1 (IDO1) mRNA, was demonstrated with clinical samples. Our method provides a strategy for the comprehensive analysis of exosomes, with the advantages of high sensitivity and practicability.
研究领域:
生物技术
·
分子生物学
·
癌症研究
·
医学诊断
✓ 已验证
DOI
PubMed
Biomaterials Science
·
2021
·Vol. 9
·期 9
·3453-3464
·
IF 6.843
Xiaofang Li,Wen Zhang,Jing Lin,Hao Wu,Yucen Yao,Jiayi Zhang,Chunrong Yang
The application of combination immune-chemotherapy makes up for the limitation of monotherapy and achieves superior antitumor activity against cancer. However, combinational therapy is always restricted by poor tumor targeted drug delivery efficacy. Herein, novel T cell membrane cloaking tumor microenvironment-responsive nanoparticles (PBA modified T cell membrane cloaking hyaluronic acid (HA)-disulfide bond-vitamin E succinate/curcumin, shortened as RCM@T) were developed. T cell membrane cloaking not only serves as a protection shell for sufficient drug delivery but also acts as a programmed cell death-1(PD-1) “antibody” to selectively bind the PD-L1 of tumor cells. When RCM@T is intravenously administrated into the blood stream, it accumulates at tumor sites and responds to an acidic pH to achieve a “membrane escape effect” and expose the HA residues of RCM for tumor targeted drug delivery. RCM accumulates in the cytoplasm via CD44 receptor mediated endocytosis and intracellularly releases antitumor drug in the intracellular redox microenvironment for tumor chemotherapy. T cell membrane debris targets the PD-L1of tumor cells for tumor immunotherapy, which not only directly kills tumor cells, but also improves the CD8+ T cell level and facilitates effector cytokine release. Taken together, the as-constructed RCM@T creates a new way for the rational design of a drug delivery system via the combination of stimuli-responsive drug release, chemotherapeutical agent delivery and cell membrane based immune checkpoint blockade immunotherapy.
研究领域:
肿瘤学
·
药理学
·
免疫疗法
·
纳米技术
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
SYNTHETIC COMMUNICATIONS
·
2018
·
IF 1.377
Xinyuan Ma,Qiong Mo,Jie Chang,Kai Xie
This paper mainly studied Pd-catalyzed regioselective chlorination of disubstituted 1,2,3-triazoles directed by the 1,2,3-triazole ring. A series of regioselective chlorinated products were synthesized in 47–86% yields using Pd(OAc)2 as a catalyst and CuCl2 as a chlorinated reagent. This method provides a new mean for the synthesis of 1,2,3-triazole halides which combines the formation of C-X bond with C-H activation.
研究领域:
有机化学
·
合成化学
✓ 已验证
DOI
高影响因子
Frontiers of Materials Science
·
2022
·Vol. 16
·期 4
·1-9
·
IF 2.612
Zhu Chaoqi,Li Xiang,Wang Xiaoxia,Su Huiyu,Ma Chaofan,Guo Xiang,Xie Changsheng,Zeng Dawen
Efficient chemical warfare agents (CWAs) detection is required to protect people from the CWAs in war and terrorism. In this work, a Pd-doped SnO 2 nanoparticles-based gas sensor was developed to detect a nerve agent simulant named methyl salicylate. The sensing measurements of methyl salicylate under different Pd doping amounts found that the 0.5 at.% Pd-doped SnO 2 exhibited a significant improvement in the detection of methyl salicylate at the ppb (1 ppb = 10 −9 ) level, and the response value to 160 ppb methyl salicylate is 0.72 at 250 °C. Compared with the pure SnO 2 , the response value is increased by 4.5 times, which could be attributed to the influence of the noble metal Pd on the oxygen state and its catalytic effect. In addition, the 0.5 at.% Pd-doped SnO 2 sensor still has an obvious response to 16 ppb methyl salicylate with a response value of 0.13, indicating the lower detection limit of the sensor.
研究领域:
材料科学
·
化学传感器
·
纳米技术
·
环境监测
✓ 已验证
DOI
高影响因子
MOLECULES
·
2016
·Vol. 21
·期 5
·618
·
IF 2.465
Dawei Li,Kelong Ao,Qingqing Wang,Pengfei Lv,Qufu Wei
Palladium nanoparticle-bacterial cellulose (PdBC) hybrid nanofibers were synthesized byin-situchemical reduction method. The obtained PdBC nanofibers were characterized by a series of analytical techniques. The results revealed that Pd nanoparticles were evenly dispersed on the surfaces of BC nanofibers. Then, the as-prepared PdBC nanofibers were mixed with laccase (Lac) and Nafion to obtain mixture suspension, which was further modified on electrode surface to construct novel biosensing platform. Finally, the prepared electrochemical biosensor was employed to detect dopamine. The analysis result was satisfactory, the sensor showed excellent electrocatalysis towards dopamine with high sensitivity (38.4 µA·mM−1), low detection limit (1.26 µM), and wide linear range (5–167 µM). Moreover, the biosensor also showed good repeatability, reproducibility, selectivity and stability and was successfully used in the detection of dopamine in human urine, thus providing a promising method for dopamine analysis in clinical application.Keywords:palladium nanoparticle;bacterial cellulose;laccase;dopamine;biosensor
研究领域:
材料科学
·
分析化学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
eScience
·
2023
·
IF 0
Mengjun Wang,Jun Jia,Hao Yan,Guang Li,Qiming Hong,Yuzheng Guo,Yong Xu,Xiaoqing Huang
Phase modulation of noble metal alloys (NMAs) is critically important in nanoscience since the distinct atomic arrangements can largely determine their physicochemical properties. However, the precise modulation of NMAs is formidably challenging, because thermodynamically stable phases are generally preferential compared to those metastable ones. Herein, we proposed a potential energy trapping strategy for phase modulation of Pd–Te alloys with solvents. Thereinto, ethylene glycol can increase the energy barrier for both Pd leaching and Te introduction, forming metastable Pd 20 Te 7 phase. Inversely, N, N-dimethylformamide is unable to trap metastable phase, inducing the phase evolution to thermodynamically stable PdTe phase, and the precise phase modulation was realized including Pd 20 Te 7 , PdTe and PdTe 2 phases. The Pd–Te alloys displayed phase-dependent formic acid oxidation catalytic performance with PdTe phase showing the best. This work proposes a strategy for creating metastable phase with potential energy trap, which may deepen the understanding of phase engineering for noble metal-based nanocrystals.
研究领域:
纳米科学
·
材料化学
·
物理化学
✓ 已验证
DOI
LANGMUIR
·
2015
·
IF 4.457
Zhihai Cao,Hangnan Chen,Shudi Zhu,Wenwen Zhang,Xufang Wu,Guorong Shan,Ulrich Ziener,Dongming Qi
Janus Pd/SiO2 nanocomposite particles (NCPs) were successfully synthesized through a combination of the sol–gel process of tetramethoxysilane in inverse miniemulsions and in situ reduction of Pd salts via a gas diffusion process of hydrazine. The formation of Pd nanoparticles (NPs) was verified by X-ray diffraction. The Janus morphology of the Pd/SiO2 NCPs was confirmed by microscopic observation. The Pd/SiO2 NCPs displayed a mesoporous structure. The content of Pd NPs in the NCPs could be conveniently adjusted by the K2PdCl4 loading. A formation mechanism of the Janus Pd/SiO2 NCPs was proposed. The mesoporous Janus Pd/SiO2 NCPs show good catalytic activity toward the reduction of p-nitrophenol with NaBH4.
研究领域:
材料科学
·
纳米技术
·
催化化学
·
胶体与界面化学
✓ 已验证
DOI
高影响因子
Applied Catalysis O: Open
·
2024
·
IF 0
Si Xiong,Aiping Wang,Baohe Wang,Jing Ma,Zhaobang Zhang,Jing Zhu
The complexation of palladium with bidentate phosphine ligand has significant effects on the activity of the catalyst for methoxycarbonylation of ethene. Herein, the influence of the ratio of components, kind and sequence of additives, complexation temperature and time on the catalytic performance were studied, then probable mechanism was proposed, and further kinetic studies were carried out under the optimized conditions. Compared to add Methanesulfonic acid (MSA) after complexation, the addition of an appropriate dose of MSA before complexation rather than after complexation could effectively improve the activity of the catalyst, while the addition of Methyl Propionate (MeP) would reduce the activity of the catalyst. It was worth noting that the weak absorption of [Pd-H] + in 1943 cm −1 was captured in the complex by in situ FT-IR.
研究领域:
催化化学
·
有机化学
✓ 已验证
DOI
JOURNAL OF HAZARDOUS MATERIALS
·
2015
·
IF 4.529
Zhenwei Gao,Yonggang Zhang,Deyi Li,Charles J. Werth,Yalei Zhang,Xuefei Zhou
The catalytic reduction of nitrate is a promising technology for groundwater purification because it transforms nitrate into nitrogen and water. Recent studies have mainly focused on new catalysts with higher activities for the reduction of nitrate. Consequently, metal nanoparticles supported on mesoporous metal oxides have become a major research direction. However, the complex surface chemistry and porous structures of mesoporous metal oxides lead to a non-uniform distribution of metal nanoparticles , thereby resulting in a low catalytic efficiency. In this paper, a method for synthesizing the sustainable nitrate reduction catalyst Pd–In/Al 2 O 3 with a dimensional structure is introduced. The TEM results indicated that Pd and In nanoparticles could efficiently disperse into the mesopores of the alumina. At room temperature in CO 2 -buffered water and under continuous H 2 as the electron donor, the synthesized material (4.9 wt% Pd) was the most active at a Pd–In ratio of 4, with a first-order rate constant ( k obs = 0.241 L min −1 g cata −1 ) that was 1.3× higher than that of conventional Pd–In/Al 2 O 3 (5 wt% Pd; 0.19 L min −1 g cata −1 ). The Pd–In/mesoporous alumina is a promising catalyst for improving the catalytic reduction of nitrate.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CATALYSIS
·
2020
·
IF 7.888
Jian Zhang,Yazhe Zhang,Jieqiong Zhao,Zhe An,Yanru Zhu,Xin Shu,Hongyan Song,Xu Xiang,Xiaodan Ma,Jing He
Oxidative carbonylation has attracted broad interest from both academia and industry in recent years. Oxycarbonylation of ethanol to diethyl carbonate (DEC) has been of great challenge due to the difficulty in the simultaneous activation of ethanol and CO. This work reports a heterogeneous Cu-Pd pair catalysis for simultaneous activation of ethanol and CO, affording a selectivity of 85.4% to DEC and a space–time yield (STY) of up to 207 mg∙g −1 ∙h −1 . Well distributed Cu-Pd pair affords more Cu (I) sites, promoting CO activation and the followed insertion to adsorbed ethanol. Further increasing ratio of Cu (I) species via the pre-treatment of catalyst with CO and ethanol promotes simultaneously the activation of ethanol and CO, affording a STY of 325 mg∙g −1 ∙h −1 of DEC.
研究领域:
化学工程
·
催化科学
✓ 已验证
DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Wang Mengjun,Jia Jun,Xia Jing,Peng Chun-Kuo,He Jinxin,Qiu Yueming,He Yuting,Gao Le,Xue Fei,Lin Yan-Gu,Zhan Guowu,Guo Yuzheng,Huang Xiaoqing,Xu Yong
Carbon dioxide (CO 2 ) conversion to liquid fuels has attracted great attention due to the current environmental concerns and energy crisis. However, the selective conversion of CO 2 to target liquids is formidably challenging due to the chemical inertness of CO 2 . We theoretically and experimentally confirm that the bending of Pd−Pd bond can breaks the asymmetric potential well and facilitate CO 2 adsorption. We have successfully synthesized a new class of Pd nanoneedles via a “close edges and open corners” process, with a magic angle of 60 o between the main trunk and branch, and realized the selective CO 2 hydrogenation to formic acid (HCOOH) at room temperature in water. Impressively, a HCOOH productivity of ~250 mmol g −1 in 100 h while maintaining HCOOH selectivity over 99%. This work bridges nanostructure design and catalytic application, which may open a new avenue for selective CO 2 conversion in an elegant manner.
研究领域:
催化
·
纳米技术
·
环境化学
·
能量转换
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Yongxin Zhang,He Li,Zhongliang Shi,Miao Guo,Yanming Hu,Guangyuan Zhou
Total hydrogenation of furfural (FAL) is an efficient way to produce the industrially important tetrahydrofurfuryl alcohol (THFOL). However, it is a challenge to obtain highly selective catalysts for this transformation especially under mild reaction conditions. Herein, we report that the β-ketoenamine-linked covalent organic framework (TpBD COF) supported ultrafine Pd nanoparticles exhibit significantly enhanced selectivity for THFOL compared to Pd/C (95% vs. 82 %) at almost full FAL conversion. This heightened selectivity is attributed to the increased electronic density on the Pd surface, resulting from the electron-donating effect of pendant groups in the TpBD COF. Moreover, kinetic investigations reveal that the activity of total FAL hydrogenation on Pd surface is linked to the rate-determining step in its first C = O hydrogenation process. Additionally, Pd/TpBD COF could catalyse various other bio-based furanic compounds to the corresponding furan ring saturated products in high yields. This work provides guidance for the rational design catalysts to improve its tandem hydrogenation efficiency.
研究领域:
化学工程
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）