EUROPEAN JOURNAL OF PHARMACOLOGY
·
2021
·
IF 4.432
Jing-Mei Lu,Guang-Nan Jin,Yu-Nan Lu,Xu-Dong Zhao,Hui-Wen Lan,Shuai-Ru Mu,Xin-Yu Shen,Guang-Hua Xu,Cheng-Hua Jin,Juan Ma,Xuejun Jin,Xiang Xu,Lian-Xun Piao
Toxoplasma gondii ( T. gondii ) is an obligate intracellular parasite that can cause liver diseases in the host, including hepatitis and hepatomegaly . High mobility group box 1 (HMGB1) is the main inflammatory mediator causing cell injury or necrosis. HMGB1 binds to toll like receptor 4 (TLR4), then activates the nuclear factor-κB (NF-κB) signaling pathway, which promotes the release of inflammatory factors. Our previous studies showed that HMGB1 mediated TLR4/NF-κB signaling pathway plays an important role in liver injury induced by T. gondii infection. Resveratrol (RSV) is a small polyphenol, which has anti-inflammatory, anti-cancer, anti- T. gondii effect. However, the effect of RSV on liver injury caused by T. gondii infection is unclear. This study used the RH strain tachyzoites of T. gondii to infect murine liver line, NCTC-1469 cells to establish an in vitro model and acute infection of mice for the in vivo model to explore the protective effect of RSV on liver injury induced by T. gondii infection. The results showed that RSV inhibited the proliferation of T. gondii in the liver, reduced the alanine aminotransferase/aspartate aminotransferase levels and pathological liver damage. Additionally, RSV inhibited the production of tumor necrosis factor-α, inducible nitric oxide synthase and HMGB1 by interfering with the TLR4/NF-κB signaling pathway. These results indicate that RSV can protect liver injury caused by T. gondii infection by intervening in the HMGB1/TLR4/NF-κB signaling pathway. This study will provide a theoretical basis for RSV treatment of T. gondii infection induced liver injury.
✓ 已验证
DOI
PubMed
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2026
·
IF 3.6
An Hongmei,Zhang Lihong,Miao Jiale,Song Aiman,Zhang Jingzhuo,Maihemuti Wulike Baike,Cao Xianfeng,Wen Yongchang,Yan Ruiying,Ren Caijin,Bao Qianjiang,Wang Jiayu,Lei Yuhang
To investigate the protective effects of three antioxidants—lycopene (LP), proanthocyanidins (PC), and resveratrol (RES)—against Nano-TiO 2 -induced reproductive toxicity in male mice. A total of 110 healthy male ICR mice were randomly assigned to 11 experimental groups: the control group, the Nano-TiO 2 group, and combined treatment groups consisting of Nano-TiO 2 with low, medium, and high doses of LP, PC, and RES, respectively. Following 28 days of continuous intragastric administration, epididymal tissues were collected for assessment of semen quality, while testicular tissues were utilized to evaluate histopathological alterations, testicular functional enzyme activities, and markers of oxidative stress. Compared with the control group, after intragastric administration of Nano-TiO 2 , the testicular and epididymal coefficients of mice decreased, the sperm count, sperm progressive motility, and sperm motility rate significantly decreased ( P < 0.05), the sperm malformation rate significantly increased ( P < 0.05), the number of spermatogenic cells in the testicular spermatogenic tubules decreased, and the Johnsen score significantly decreased ( P < 0.05). The activities of acid phosphatase (ACP), alkaline phosphatase (AKP), lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione (GSH) decreased significantly ( P < 0.05). The level of malondialdehyde (MDA) was significantly increased ( P < 0.05). After the intervention with the three antioxidants, compared with the Nano-TiO 2 group, the testicular and epididymal coefficients increased. Compared with other doses, medium-dose LP, medium-high-dose PC, and all doses of RES significantly increased the forward motility and sperm motility of mice and significantly reduced the sperm deformity rate( P < 0.05). In addition, various doses of LP, medium - high- doses of PC, and low- doses RES significantly improved pathological damage caused by Nano-TiO 2 , such as testicular tissue structural disorder and decreased levels of spermatogenic cells and sperm. The activities of ACP, LDH, SOD, CAT, GSH, and GPx increased significantly ( P < 0.05), and the level of MDA in the testicular tissue decreased significantly. LP, PC, and RES had antagonistic effects on male reproductive toxicity induced by Nano-TiO 2 , the prominent effects of PC on the testicular organ coefficient and LP, especially 20 mg/kg LP, in improving semen quality and the expression of testicular functional enzymes in male mice need to be given due attention.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEUROSCIENCE LETTERS
·
2026
·
IF 2.2
Wuwei Xu,Yanxin Xu,Chunyan Yao,Xianmei Fu,Xiaoling Liu,Jinping Fan,Kexue Wang,Yumeng Zhou,Yuefei Li,Ting Hu,Yan Sai,Peng Luo,Tongjian Cai
T-2 toxin is a widely distributed environmental mycotoxin that poses severe neurotoxic risks to humans and animals. Neuroinflammatory responses constitute a key mechanism underlying the neurotoxicity of T-2 toxin. However, its molecular mechanisms for inducing inflammatory responses in the nervous system remain incompletely understood. This study investigated the role of B-cell translocation gene 2 (BTG2) in T-2 toxin-induced microglial activation and evaluated the neuroprotective potential of resveratrol (Res). In vivo experiments demonstrated that exposure to T-2 toxin resulted in spatial learning, memory impairments and locomotor deficits in mice. These behavioral phenotypes were accompanied by neuroinflammation in the hippocampus and cerebral cortex, characterized by microglial activation and the upregulation of BTG2. Res pre-treatment effectively reversed these neurobehavioral deficits and suppressed the neuroinflammatory response. Mechanistic analyses revealed that Res considerably alleviated T-2 toxin-induced TLR4/MyD88/NF-κB pathway activation. In vitro studies using BV-2 microglial cells confirmed these findings. Notably, siRNA-mediated knockdown of BTG2 also abolished TLR4 pathway activation and subsequent cytokine expression induced by T-2 toxin, establishing BTG2 as a critical upstream regulator. Furthermore, RNA sequencing systematically verified that BTG2 regulates the immune response network, specifically targeting the TLR4 cascades. Collectively, these findings provide novel evidence that Res may target the BTG2-regulated TLR4/MyD88/NF-κB signal pathway to exert neuroprotection, providing a promising intervention option for reducing the environmental and health hazards associated with exposure to T-2 toxin
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 6
·369
·
IF 5.4
Ding Le,Bi Huifang,Liu Hanqing,Xu Jing,Wang Tao,Zhou Haifeng,Zhou Guangjun
A novel dual-signal sensing platform is developed for the simultaneous quantitative determination of resveratrol (Res) and ascorbic acid (AA), integrating fluorescence-based quantification with colorimetric visual recognition, with a sensing mechanism based on the inner filter effect (IFE) of a composite system consisting of upconversion nanoparticles (UCNPs), 3,3’,5,5’-tetramethylbenzidine (TMB), and ferric ions (Fe³⁺). In this system, Fe³⁺ first oxidizes colorless TMB to blue oxidized TMB (oxTMB), and the formed oxTMB further quenches the fluorescence of the UCNPs through the IFE. When Res or AA is introduced into the system, oxTMB is reduced back to colorless TMB, which results in the fading of the blue solution and the recovery of UCNP fluorescence, thus achieving the dual-signal detection of the two analytes through fluorescence-based quantification and colorimetric qualitative determination. Furthermore, 4-hydroxy-TEMPO is introduced as an AA-specific scavenger, which can effectively eliminate the interference of AA and achieve selective discrimination between Res and AA. This developed sensing platform integrates the prominent merits of simple operation, high sensitivity, excellent specificity, and visual readout. It provides not only a novel analytical strategy for the efficient detection of Res and AA but also broad application prospects in the field of analytical chemistry. Graphical abstract The alternative text for this image may have been generated using AI.
✓ 已验证
DOI
PubMed
高影响因子
Molecular Catalysis
·
2026
·
IF 4.3
Tao Yao,Miaolian Ma,Chengke Wang,Wanpeng Zhou,Maofeng Zhang
A novel metal-free catalyst is used for fast H 2 generation from NaBH 4 methanolysis. • Reaction kinetics order of resveratrol-catalyzed NaBH 4 methanolysis was identified. • The E a was calculated to be 25.69 kJ/mol within the temperature range of 248∼288 K. • Resveratrol as catalyst exhibits excellent reusability and regeneration performance. In this study, natural polyphenolic compounds (including resveratrol, naringenin, hesperetin, and tea polyphenol) were employed as catalysts for hydrogen production from methanolysis of NaBH 4 , and the reaction conditions were systematically investigated. The effects of catalyst type, NaBH 4 concentration, catalyst dosage, and reaction temperature on the catalytic performance were examined individually. The results demonstrated that resveratrol exhibited the best catalytic activity. At 25 °C, with 0.05 g of resveratrol as the catalyst for methanolysis of 0.1 g NaBH 4 , the hydrogen generation rate (HGR) reached up to 3926 mL H 2 g cat −1 min −1 . Even at -25 °C, the HGR value could reach 420 mL H 2 g cat −1 min −1 , indicating favorable low-temperature catalytic performance. The activation energy for methanolysis of NaBH 4 catalyzed by resveratrol was calculated to be 25.69 kJ mol −1 over the temperature range of -25 to 15 °C. Reusability and regeneration tests showed that the catalyst retained high activity after five consecutive cycles. Download: Download high-res image (180KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
Food & Function
·
2026
·
IF 6.3
Fujia Chen,Siqiang Li,Shuaihui Jia,Mengjie Lu,Lumei Dai,Shuang Guo,Xiyu Hu,Hongwei Guo,Enzhong Li
Resveratrol, a dietary polyphenol from grapes and berries, was evaluated for its protection against high-fat diet (HFD)-induced testicular dysfunction via the gut-microbiota-testis axis. In mice, resveratrol improved sperm quality and systemic metabolism. It reshaped gut microbiota (enriching Bifidobacterium) and normalized testicular metabolites, including the antioxidant 1,4-dithiothreitol and membrane-stabilizing heptadecanoic acid. Crucially, network pharmacology predicted specific targets, TNF-α, IL-1β, PTGS2, HRAS, and the TCA cycle enzyme SDHB, which led us to hypothesize and validate that resveratrol enhances mitochondrial TCA cycle activity (upregulating SDHB, ACO2, CS, MDH2). Concurrently, it suppressed the NF-κB pathway and promoted repair processes. Molecular docking confirmed stable binding to these targets. This work demonstrates that dietary resveratrol alleviates testicular impairment, a mechanism pinpointed through computational prediction, supporting its role as a nutritional intervention for male subfertility.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nutrients
·
2022
·Vol. 14
·期 12
·2413
·
IF 6.706
Jiaqiao Li,Yameng Fan,Yan Zhang,Yamei Liu,Yan Yu,Mao Ma
Resveratrol (RSV) has been reported to induce autophagy and apoptosis in non-small-cell lung cancer A549 cells, and the nerve growth factor receptor (NGFR) regulates autophagy and apoptosis in many other cells. However, the effect of NGFR on autophagy and apoptosis induced by RSV in A549 cells remains unclear. Here, we found that RSV reduced the cell survival rate in time- and concentration-dependent manners, activating autophagy and apoptosis. Lethal autophagy was triggered by RSV higher than 55 μM. The relationship between autophagy and apoptosis depended on the type of autophagy. Specifically, mutual promotion was observed between apoptosis and lethal autophagy. Conversely, cytoprotective autophagy facilitated apoptosis but was unaffected by apoptosis. RSV enhanced NGFR by increasing mRNA expression and prolonging the lifespan of NGFR mRNA and proteins. RSV antagonized the enhanced autophagy and apoptosis caused by NGFR knockdown. As the downstream pathway of NGFR, AMPK-mTOR played a positive role in RSV-induced autophagy and apoptosis. Overall, RSV-induced autophagy and apoptosis in A549 cells are regulated by the NGFR-AMPK-mTOR signaling pathway.Keywords:resveratrol;non-small-cell lung cancer;A549 cell line;autophagy;apoptosis;NGFR-AMPK-mTOR pathway
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY
·
2021
·
IF 4.221
Yujie Huang,Jie Zhang,Yizhou Tao,Cheng Ji,Stanley Aniagu,Yan Jiang,Tao Chen
Benzo[a]pyrene (BaP), a prototypical polycyclic aromatic hydrocarbon, is widely present in the environment. BaP-induced heart defects have been frequently reported, but the underlying molecular mechanisms remain elusive. Here, we found that BaP increased heart malformations in zebrafish embryos in a concentration-dependent manner, which were attenuated by supplementation with either CH223191 (CH), an aryl hydrocarbon receptor (AHR) inhibitor, or N-acetyl- l -cysteine (NAC), a reactive oxygen species (ROS) scavenger. While CH and NAC both inhibited BaP-induced ROS generation, NAC had no effect on BaP-induced AHR activation. We further demonstrated that BaP increased mitochondrial ROS, decreased mitochondrial membrane potential, and caused endogenous apoptosis, with all these effects being counteracted by supplementation with either CH or NAC. Resveratrol (RSV), a natural AHR antagonist and ROS scavenger, also counteracted the heart malformations caused by BaP. Further experiments showed that RSV attenuated BaP-induced oxidative stress, mitochondrial damage and apoptosis, but had no significant effect on AHR activation. In conclusion, our findings show that BaP induces oxidative stress via AHR activation, which causes mitochondria-mediated intrinsic apoptosis, resulting in heart malformations in zebrafish embryos, and that RSV had a protective effect against BaP-induced heart defects mainly by inhibiting oxidative stress rather than through antagonism of AHR activity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Xin Yin,Huanhuan Dong,Hao Cheng,Chuye Ji,Li Liang
In oil-in-water emulsions, a single phenolic compound has been used as interfacial antioxidant. The co-encapsulated polyphenols may produce more supper stability or bioactivity than a single encapsulated one. This study used epigallocatechin-3-gallate (EGCG) and resveratrol as model polyphenols with different solubility. Their co-encapsulation in SC particles was analyzed on the basis of particle characterization, loading efficiency, antioxidant activity , polyphenolic microenvironment and retention. When the polyphenols’ concentration was 0.4 mM, 98% EGCG and 68% resveratrol were co-encapsulated in sodium caseinate (SC) particles. The SC particles containing dual polyphenols were then used to stabilize fish oil emulsions, which were analyzed in term of particle characterization, oil oxidation , polyphenolic partition and retention. When the content of fish oil was 10%, there were 77% resveratrol in the emulsified oil droplets and 87% EGCG at the oil-water interface. The co-encapsulated EGCG improved the stability of resveratrol in SC particles and protected fish oil emulsions against oxidation prior to resveratrol. These results are instructive for the combination of multiple bioactive compounds for the applications in foods and pharmaceuticals.
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2021
·Vol. 13
·期 2
·846-856
·
IF 5.396
Weidong Wang,Zenggen Liu,Fan Kong,Lixia He,Linghao Fang,Qingyan Shu
Tree peonies are well-known horticultural and medicinal plants. The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities. In this study, the phytochemicals isolated from tree peony seed coats were systematically investigated. Seven polyphenolics were separated and prepared, mainly belonging to resveratrol derivatives. There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species. Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among Paeonia plants was found, including the composition patterns and contents of the constituents. Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice. In addition, in vitro enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC50 values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and trans-ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbial Biotechnology
·
2020
·Vol. 13
·期 4
·1118-1131
·
IF 5.328
Tao Ma,W. Wu,Y. Tu,N. Zhang,Q. Diao
Summary This study aimed to investigate the effect of resveratrol on methane production, rumen fermentation and microbial composition under high-concentrate (HC) and high-forage (HF) diets using the in vitro fermentation system. A total of 25 mg of resveratrol was supplemented into 300 mg of either HC or HF diet. Methane production, total volatile fatty acid (VFA) concentration, molar proportion of VFA, metabolites of resveratrol and prokaryotic community composition were measured after 12 and 24 h of in vitro fermentation. Resveratrol reduced methane production (ml per mg of dry matter degraded) by 41% and 60% under both HC and HF diets ( P < 0.001), respectively, and this result could be associated with the lower abundance of Methanobrevibacter ( P < 0.001) in response to resveratrol. The molar proportion of propionate was significantly higher in the resveratrol group only under the HC diet ( P = 0.045). The relative abundance of 10 bacterial genera was affected by the three-way interaction of treatment, diet and time ( P < 0.05). Resveratrol was partly converted to dihydroresveratrol after 24 h of fermentation, and its degradation could be associated with microbes belonging to the order Coriobacteriales . Our results suggest that multiple factors (e.g. diet and time) should be considered in animal experiments to test the effect of polyphenol or other plant extracts on rumen fermentation, methane emission and microbial composition.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Zheng Fang,Xixi Cai,Jiulin Wu,Lingtuo Zhang,Yapeng Fang,Shaoyun Wang
Plant protein-based nano-delivery systems have rencently drawn increasing attention, and several physical and chemical methods for improving the functional properties of plant proteins have been reported. In this work, we applied ultrasonication and pH-shifting combined treatment on soy protein isolates (SPI) and investigated the structure changes of SPI and its effects on the formation of SPI- sodium alginate (Alg) electrostatic complex and SPI-resveratrol nanocomplex. It was found that the combined ultrasonication/pH-shifting treatment resulted in a smaller particle size and larger surface hydrophobicity, which were 114.6 ± 1.2 nm and 12741 ± 456, respectively. During the formation of the complex with Alg , compared with pH-shifting or ultrasonication treatment, the combined ultrasonication/pH-shifting treatment expanded stable region, where the particle size was smaller and the binding constant with sodium alginate was higher. Moreover, the encapsulation efficiency of resveratrol in SPI increased to 91.4 ± 4.3% after the combined ultrasonication/pH-shifting treatment. Thus, simultaneous treatment combining ultrasonication and pH-shifting was significantly more effective than ultrasonication alone and was thought as an effective method for protein modification.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）