INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Peng Wang,Zhi-gang Luo,Zhi-gang Xiao,Ahmed S.M. Saleh
Oxidized gellan gum (OGG) hydrogel beads as delivery systems for resveratrol were fabricated by ionic cross-linking with calcium chloride (CaCl 2 ). The degree of oxidation (DO) and CaCl 2 concentration had significant influences on the formation and functional properties of hydrogel beads. The resveratrol encapsulation efficiency (66.43%–79.84%) and loading capacity (4.15%–5.05%) of OGG hydrogel beads were enhanced as DO increased. The hydrogel beads exhibited a uniform spherical shape as observed by scanning electron microscope. Fourier transform infrared spectroscopy analysis confirmed that hydrogen bonds and ionic interaction participated in the formation of hydrogel beads. X-ray diffraction analysis revealed that the physical state of resveratrol was changed from crystalline to amorphous form after encapsulation. Furthermore, the physical stability and antioxidant capacity evaluation demonstrated that the hydrogel bead fabricated with DO80 OGG and CaCl 2 concentration of 1.0 M could provide high protection for resveratrol against degradation by environmental stresses and maintain its antioxidant capacity. The DO and CaCl 2 concentrations could modulate the in-vitro release behaviors of hydrogel beads and obtain a good small intestinal-targeted release of resveratrol at high DO and medium CaCl 2 concentration. These findings suggested that a promising delivery system for encapsulating bioactive ingredients can be fabricated by rational design.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
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IF 6.953
Can Song,Feihong Huang,Linyu Liu,Quan Zhou,Dan Zhang,Qi Fang,Hui Lei,Hong Niu
Pectins have proven to be advantageous for human health as they regulate beneficial microbial communities and enhance immunity. The fruit of Clausena lansium (Lour.) Skeels (Wampee), also referred to as “treasure in fruit”, is rich in pectin polysaccharides . In this study, a homogalacturonan-type pectin (CCP2) with a molecular weight of 8.9 × 10 4 Da and degree of esterification of 42.86% was isolated from Wampee fruit. The gut microbiota regulation and phagocytosis-enhancing properties of CCP2 were examined in vivo and in vitro, respectively. Oral administration of CCP2 dramatically decreased the abundance of Bacteroidetes and increased the abundance of Firmicutes in intestinal bacteria in mice. The content of short-chain fatty acids in the feces also significantly improved. Moreover, CCP2 exhibited excellent phagocytosis-enhancing activities on RAW 264.7 macrophages. These results suggested that CCP2 could be a potential gut microbiota regulator and phagocytosis-enhancer, which could be used in food products to promote health through beneficial manipulation of gut microbiota.
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CARBOHYDRATE POLYMERS
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2021
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IF 9.381
Tingting Zhang,Hualin Wang,Dongxiu Qi,Li Xia,Linlin Li,Xingjiang Li,Shaotong Jiang
A colorimetric cellulose acetate (CA) membrane incorporated with Perilla frutescens (L.) Britt. anthocyanins (PFA) and chamomile essential oil (CO) is developed via electrospinning technique for food freshness monitoring and shelf-life extending. The moieties of PFA and CO are well-dispersed in fiber matrix by hydrogen bonds and their incorporation increases the fiber size but with no obvious influence on the fiber morphology at incorporation levels. The presence of CO enhances membrane hydrophobicity . The target membrane of CA-PFA6-CO15 (PFA6%, CO15%) has a wide color change range of pH 2–12 which is high sensitive and reversible towards external pH-stimuli. The membrane has good antibacterial activity against E. coli and S. aureus besides antioxidant activity . The release of bioactive moieties is predominantly controlled by Fickian diffusion. The target membrane can simultaneously monitor pork freshness in real-time and double the shelf-life at 25 °C, indicating its potential application in active and intelligent food packaging.
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CHEMICAL ENGINEERING JOURNAL
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2021
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IF 13.273
Xin Zuo,Bohyung Kim,Baibai Liu,Dongmei He,Le Bai,Wenqi Wang,Cunyun Xu,Qunliang Song,Chunyang Jia,Zhigang Zang,Donghwa Lee,Xiong Li,Jiangzhao Chen
The interfacial carrier nonradiative recombination resulting from defects and energy barrier at buried interface hinders further enhancement of power conversion efficiency (PCE) and stability of perovskite solar cells. Herein, we report a simple and effective buried interface passivation strategy based on cation engineering through employing a new type of sulfonium salt ((2-carboxyethyl) dimethyl sulfonium chloride, CDSC) together with reference molecule (3-dimethylamino propionic acid hydrochloride, DPAH) to modify the interface between perovskite and electron transport layers. It is theoretically and experimentally revealed at the atomic scale that CDSC and DPAH chemically interact with both SnO 2 and perovskite layer and accordingly well bridge both layers. Both modifiers can not only passivate the defects from the surface of perovskite and SnO 2 films, but also reduce interfacial energy barrier via improving energy band alignment. CDSC are certified to be more effective in defect passivation and energy band modulation than DPAH, for the first time revealing that sulfonium cations are superior to commonly adopted ammonium cations. Finally, the DPAH and CDSC-modified devices achieve a PCE of 21.44% and 22.22%, respectively, far outperforming the control device (20.72%). The unsealed devices with CDSC maintain 92.5% of their initial efficiency after thermal aging for 1272 h.
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CHEMICAL ENGINEERING JOURNAL
·
2021
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IF 13.273
Jiabin Chen,Jie Xu,Tongcai Liu,Qi Wang,Nan Li,Yalei Zhang,Libin Yang,Xuefei Zhou
Peracetic acid (PAA) is an emerging oxidant and disinfectant increasingly used in wastewater treatment. Tetracyclines (TCs) are extensively consumed antibiotics, and frequently detected in the wastewater and surface water. Herein, PAA was found to show high reactivity towards TCs for the first time. This reaction follows the second-order kinetics with the rate constant ( k 2,app ) rapidly raised from pH 5 to 7, and then slightly increased to pH 9. Degradation of TCs by PAA was slightly influenced by water components, thus maintaining high efficiency in the real waters. Theoretical calculations revealed the electrons mainly concentrated on the B-ring of TCs, implying the B-ring as the reactive moieties for PAA oxidation. Transformation product analysis confirmed that oxidation of TCs by PAA mainly proceeded via hydroxylation and N-demethylation. Finally, PAA-promoted oxidation could remarkably reduce the antibacterial activity of TCs.
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MOLECULAR PHARMACEUTICS
·
2021
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IF 4.939
Xiaoxia Yang,Pengfei Shang,Jianbo Ji,Christina Malichewe,Zhiyin Yao,Jing Liao,Dandan Du,Chao Sun,Lei Wang,Ya-jie Tang,Xiuli Guo
In this study, a chitosan-based, self-assembled nanosystem that codelivered microRNA34a (miR34a) and doxorubicin (Dox) with hyaluronic acid (HA) modification (named CCmDH NPs) was developed to reverse the resistance of breast cancer (BCa) cells to Dox. The CCmDH NPs had a diameter of 180 ± 8.3 nm and a ζ potential of 16.5 mV with a slow-release effect for 96 h. The codelivery system could protect miR34a from nuclease and serum degradation and transport miR34a and Dox into drug-resistant MCF-7/A cells. In addition, the CCmDH NPs could inhibit proliferation and promote apoptosis by regulating the protein expression of B-cell lymphoma-2 (Bcl-2) and poly(ADP-ribose) polymerase (PARP) and inhibit invasion, metastasis, and adhesion by regulating E-cadherin, N-cadherin, MMP2, CD44, and Snail molecules. The CCmDH NPs induced a 73.7% tumor reduction in xenograft tumor growth in nude mice in vivo. This study provides evidence for the anticancer activity of CCmDH NPs carrying Dox and miR34a in BCa, especially metastatic Dox-resistant BCa models.
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CHEMICAL ENGINEERING JOURNAL
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2021
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IF 13.273
Desheng Liu,Pan Jiang,Xin Xu,Jiayu Wu,Yaozhong Lu,Xinzhong Wang,Xiaolong Wang,Weimin Liu
Developing high-performance catalytic filter with both high removal efficiency and ultra-high flux, especially under gravity-driven conditions, is of great significance yet still a fundamental challenge in organic wastewater purification. Herein, a high-efficiency gravity-driven catalytic filter, ZIF-67 decorated sugarcane that is denoted as ZIF-67@SC sponge, is successfully constructed by the in-situ growing ZIF-67 nanoparticles inside the channels of biomass sugarcane skeleton. Pretreatment of cellulose fibers with alkali can set the nucleation sites for the ZIF-67 nanocrystals decoration on the sugarcane skeleton, which originates from the ion exchange between Co 2+ and Na + . The effect of the perforated plate structures of sugarcane on fluid obstruction and the widely distributed ZIF-67 nanoparticles can synergistically enhance the contraction between organic pollutants and ZIF-67 nanoparticles. The resultant ZIF-67@SC sponge therefore exhibits the superior removal efficiency up to 86.99 % for methylene blue (MB) in water with the water treatment flux of ∼ 2720 L m -2 h −1 under the activation of peroxymonosulfate (PMS). Importantly, the ZIF-67@SC sponge catalytic filter can be readily regenerated by a simple methanol washing, maintaining the removal efficiency of 82.09 % after even 10 continuous regeneration cycles. Furthermore, the ZIF-67@SC sponge catalytic filter can be facilely engineer as a continuous-flow catalytic reactor like a water purifier for high throughout wastewater purification. It’s believed that relying on the outstanding catalytic performance of metal–organic frameworks (MOFs) and the unique skeletons of sugarcane and other biomass materials, the present outcomes can be promising by extended to the large-scale water remediation.
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ACS Applied Materials & Interfaces
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2021
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IF 9.229
Haiyang Wang,Wenbo Wang,Lu Liu,Meng Wang,Guowei Li,Hang Li,Bo Li,Siming Yu,Dong Ma,Wei Xue
Photodynamic therapy (PDT) has attracted extensive attention in the clinical treatment of malignant tumor. However, the acidic and hypoxic conditions of the tumor microenvironment (TME) limit the further application of PDT in the clinic. Herein, we fabricate a new nanoplatform─HPDA@MnO2@Ce6/DOX@PEG-RGD (HPMRCD)─by means of coating hollow polydopamine nanoparticles (HPDA) with manganese dioxide (MnO2), which is modified by cyclic RGD functionalized poly(ethylene glycol) (PEG) and further co-loaded with a photosensitizer, Chlorin e6 (Ce6), and a chemotherapy drug, doxorubicin (DOX). This nanoplatform could be enriched in tumor tissues, then instantly dissociated under an acidic and H2O2-rich TME. The dual-responsive release of Mn2+ ions and oxygen (O2) can relieve tumor hypoxia, which can be used as a magnetic resonance contrast agent and the latter can enhance the PDT effect. Furthermore, the degradation of HPMRCD leads to an efficient loaded therapeutic molecule release, thus yielding a potential therapy to enhance tumor suppression by adopting the combined chemo-photodynamic therapy.
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Journal of the Taiwan Institute of Chemical Engineers
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2021
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IF 5.876
Jiaxin Huang,Yatian Zhang,Chunxian Tao,Shanni Li,Qingxiang You,Dawei Zhang,Zhenqing Li,Yoshinori Yamaguchi
Background Capillary electrophoresis (CE) is an effective method for protein analysis. Although previous researchers have proved that the separation performance of large DNA molecules was improved by pulsed field capillary electrophoresis (PFCE), there is lack of research about proteins separation by PFCE. Methods Herein, we systematically investigated the separation performance of proteins sized from 44 kDa to 200 kDa under square wave and inversion electric field, respectively. Findings Results showed that for pulsed electric field with same forward and backward time duration, there was a resonance frequency for protein molecule re-orientation and applied frequency, and the apparent mobility ( μ ) was the lowest at the resonance frequency. μ was positively correlated to modulation depth ( M ) if M was below 200%, while μ was negatively correlated to M if M was higher than 200%. For CE with same forward and backward voltage, it demonstrated that μ dropped as M went up, but the separation performance for proteins with high molecular mass improved. Through PFCE, albumin and α- globulins in human serum were also resolved within 7 min. Such a work may shed new light on the analysis of proteins by PFCE.
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JOURNAL OF MEDICINAL CHEMISTRY
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2021
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IF 7.446
Chutong Tian,Jingjing Guo,Yifan Miao,Shunzhe Zheng,Bingjun Sun,Mengchi Sun,Qing Ye,Wenxue Liu,Shuang Zhou,Ken-ichiro Kamei,Zhonggui He,Jin Sun
Off-target drug release and insufficient drug delivery are the main obstacles for effective anticancer chemotherapy. Prodrug-based self-assembled nanoparticles bioactivated under tumor-specific conditions are one of the effective strategies to achieve on-demand drug release and effective tumor accumulation. Herein, stimuli-activable prodrugs are designed yielding smart tumor delivery by combination of the triglyceride-mimic (TG-mimetic) prodrug structure and disulfide bond. Surprisingly, these prodrugs can self-assemble into uniform nanoparticles (NPs) with a high drug loading (over 40%) and accumulate in tumor sites specifically. The super hydrophobic TG structure can act as a gate that senses lipase to selectively control over NP dissociation and affect the glutathione-triggered prodrug activation. In addition, the impacts of the double bonds in the prodrug NPs on parent drug release and the following cytotoxicity, pharmacokinetics, and antitumor efficiency are further demonstrated. Our findings highlight the promising potential of TG-mimetic structure-gated prodrug nanoparticles for tumor-specific drug delivery.
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ACTA BIOCHIMICA ET BIOPHYSICA SINICA
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2021
·Vol. 53
·期 12
·1590-1601
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IF 3.848
Yu Guili,Wang Jinxin,Zhang Wei,Yang Qiankun,Liu Gang,Wang Lei,Bello Babatunde Kazeem,Zhang Xiao,Zhang Tianmeng,Fan Hui,Zhao Panpan,Liang Wei,Dong Jingquan
Vibrio harveyi, an important zoonotic pathogen, can infect wounds and cause inflammatory response. Understanding the inflammatory response pathways could facilitate the exploration of molecular mechanisms for treating V. harveyi infection. NLR family pyrin domain-containing 3 (NLRP3) inflammasome is involved in the interaction between hosts and pathogenic microorganisms and could be sensed by various pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs). Nonetheless, the function of NLRP3 inflammasome in V. harveyi infection remains unclear. In the present study, we established a V. harveyi infection model using murine peritoneal macrophages (PMs). Various techniques, including western blot analysis, enzyme-linked immunosorbent assay (ELISA), RT-qPCR, immunofluorescence, and inhibition assays, were used to explore the molecular mechanism of V. harveyi-induced inflammation. The results showed that many inflammatory cytokines participated in V. harveyi infection, with interleukin (IL)-1β being the most abundant. Pan-caspase inhibitor pretreatment significantly decreased the secretion of IL-1β in murine PMs. Moreover, the identification of V. harveyi involved a large number of NLR molecules, especially the NLRP3 receptor, and further studies revealed that NLPR3 inflammasome was activated by V. harveyi infection, as evidenced by puncta-like NLRP3 surrounding cell nuclear, ASC specks in the nucleus and cytoplasm, and ASC oligomerization. Inhibition of NLRP3 inflammasome impaired the release of mature IL-1β in V. harveyi-infected murine PMs. Furthermore, blocking the secretion of mature IL-1β could markedly decrease the release of other proinflammatory cytokines, including IL-6, IL-12, and tumor necrosis factor-α. Overall, these data indicated that NLRP3 inflammasome was activated in response to V. harveyi infection and enhanced inflammatory response by promoting IL-1β secretion in murine PMs.
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JOURNAL OF FOOD SCIENCE
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2021
·Vol. 86
·期 10
·4262-4274
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IF 3.167
Hui Xue,Meng Xu,Guowen Zhang,Feng Feng,Yuting Wang,Danhui Cao,Yonggang Tu,Yan Zhao
This study aimed to investigatethe mechanism of stewing with tea polyphenols (TP) on the properties of boiled egg white gel (BEWG). The results indicated that, during the stewing process, soluble protein and hardness showed an overall increasing trend, while surface hydrophobicity showed a decreasing trend with blue-shift. The free sulfhydryl group showed that TP could promote the formation of disulfide bonds, and the position of immobilized water at T 2 showed a decreasing trend. Environmental scanning electron microscopy and SDS-PAGE showed that the protein gel aggregation degree increased. Moreover, Fourier transform infrared spectrometry showed that protein polarity increased and that α-helices, β-turn, intramolecular β-sheets, as well as intermolecular antiparallel β-sheets showed an increasing trend. Generally, TP strengthened protein aggregation by promoting the formation of disulfide and hydrogen bonds, thus enhancing the gel strength of BEWG. Moreover, the secondary structure of proteins became more stable under the action of TP, and the higher the concentration of TP, the greater the effect on BEWG. Practical Application TP, an ideal, cheap, and safe natural food additive, can be applied to the processing of egg products because the addition of TP can significantly improve the gel strength of egg white.
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