JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 20
·14063-14074
·
IF 2.993
Zhou Junyi,Tang Qiuju,Zhong Jiaxing,Lei Zhentao,Luo Haipeng,Tong Zaizai,Jiang Guohua,Liu Xiangdong
Herein, glucose and H 2 O 2 stimuli-responsive vesicles are constructed based on host–guest interaction between a diblock copolymer,
poly(ethylene glycol)- b -poly[3-acrylamidophenylboronic acid- co -4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl acrylate], (PEG- b -P(PBA- co -PBEM), BCP for short) and α-cyclodextrin.
In the presence of glucose, the vesicles are transformed to giant swollen spherical micelles because of the formation of a negatively charged tetravalent form between phenylboronic acid and glucose. On the other hand, the vesicles are totally disassembled when they are exposed to H 2 O 2 , which is due to the H 2 O 2 -mediated degradation of the pendant phenylboronic acid pinacol ester. The glucose and H 2 O 2 stimuli-responsive vesicles are then applied in the controlled release of water-soluble anticancer drug, doxorubicin hydrochloride (DOX). Upon external stimuli, the DOX displays a faster release rate than that without stimuli. Moreover, the polymeric vesicles show an excellent cytocompatibility toward MCF-7 cells, and the drug-loaded vesicles exhibit a lower cytotoxicity than free drug toward cancer cells. The drug-loaded vesicles can be taken up by MCF-7 cells and further release the DOX in cancer cells due to the high glucose and H 2 O 2 concentration in tumor cells, while they have negligible effect on normal cells, which may be important for applications in the therapy of cancers as a controlled-release drug carrier.
✓ 已验证
产品关联
DOI
高影响因子
Forensic Toxicology
·
2018
·Vol. 37
·期 1
·121-131
·
IF 3.924
Chen Lizhu,Yu Yingjia,Wang Yang,Xiang Ping,Duan Gengli
Purpose It is crucial for forensic analysis to differentiate clinical use from illegal abuse. Selegiline (SG) is mainly metabolized to desmethylselegiline (DM-SG), R -(−)-methamphetamine (R-MA) and R-(−)amphetamine (R-AM); while abused methamphetamine and amphetamine mainly contain the S -(+)-form. The aim of this study was to simultaneously determine SG, DM-SG, R / S -MA, and R / S -AM in human oral fluid (OF) and to differentiate clinical use from illegal use. We also aim to apply the present method to the OF samples from authentic cases in forensic toxicology. Methods Liquid–liquid extraction and liquid chromatography-tandem mass spectrometry operating in positive ion multiple-reaction monitoring mode were utilized. The chromatographic system consisted of a Chirobiotic TM V2 column (2.1 mm × 250 mm, 5 μm) and a mobile phase of methanol containing 0.1% (v/v) glacial acetic acid and 0.02% (v/v) ammonium hydroxide. Results and conclusions The calibration curves were linear from 1 to 100 ng/mL, and r > 0.995 for all analytes with imprecisions ≤ 15% and accuracy between 85 and 115%. Extraction recoveries ranged from 46.3 to 104.7% with coefficient of variation (CV) ≤ 10.3% and matrix effects (MEs) ranged from 47.4 to 114.8% with CV ≤ 12.9%. The lower limit of quantification was 0.2 ng/mL for SG and DM-SG and 1.0 ng/mL for S-MA, R-MA, S-AM and R-AM. The present method is simple, rapid (accomplished in 12 min), sensitive, and validated by authentic samples.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-9
·
IF 5.705
Wang Lude,Chen Yang
A colorimetric and fluorescent pH probe was designed by doping carbon dots (C-dots) with Eu(III), Tb(III) and 2,6-pyridinedicarboxylic acid (DPA). The resulting nanoparticles were applied as fluorescent indicators for pH values (best detected at excitation/emission wavelengths of 272/545, 614 nm). The pH induced optical effects are due to pH induced variations in energy transfer. The fluorescence of the probe shows a continuous color variation, and a linear change with pH values in the range from 3.0 to 10.0 can be established by using a Commission Internationale de L’Eclairage (CIE) chromaticity diagram. This new kind of pH nanoprobe is more accurate than previously reported pH indicator probes because the pH value can be calculated by using chromaticity coordinates that only depend on the chromaticity. The pH nanoprobe was applied to visualize pH values in human breast adenocarcinoma cells (MCF-7).
Carbon dots modified with Eu(III) and Tb(III) complexes of 2,6-pyridinedicarboxylic acid (DPA) were prepared. The doped carbon dots were used as a pH-sensitive nanosensor. The fluorescence chromaticity of the nanoparticles changes with the variation of pH value.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-10
·
IF 5.705
Huang Yan-Qin,Yang Li-Na,Wang Yong-Sheng,Xue Jin-Hua,Chen Si-Han
The authors report on a one-pot approach for synthesizing highly fluorescent protamine-stabilized gold nanoclusters. These are shown to be a viable nanoprobe for selective and sensitive fluorometric determination of lead(II) via quenching of fluorescence via Pb(II)-Au(I) interaction. Under optimized conditions, fluorescence measured at excitation/emission peaks of 300/599 nm drops in the 80 nM–15 μM lead(II) concentration range. The detection limit is 24 nM, and relative standard deviations (for n = 11) at concentrations of 0.10, 4.0 and 15 μM are 1.6, 2.5 and 1.9%, respectively. The relative recoveries of added lead(II) in the water samples ranged from 97.9 ± 2.29% to 101.2 ± 1.83%.
Lead(II) ions are found to be able to selectively and sensitively quench the fluorescence of the protamine-gold nanoclusters (PRT-AuNCs). Thereby, an inexpensive, selective and sensitive lead(II) assay was established.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR RESEARCH
·
2018
·Vol. 26
·期 11
·1035-1041
·
IF 1.767
Fu Shuqin,Zhu Jiaping,Chen Shaojun
As a new type of smart polymers, the shape memory polyurethanes attract increasing attention because of their wide use in various applications. Herein, a serials of novel thermo-responsive shape memory polyurethane networks were developed by chemical cross-linking of polycaprolactone with 1,3,5,7-tetrahydroxyadamantane. The polyurethane networks were characterized in relation to their microstructure, cross-linking density, surface appearance, thermal properties, static and dynamic mechanical properties, and shape memory behavior. It was found that the prepared polyurethane networks can exhibit excellent thermo-responsive shape memory properties at body temperature by controlling the molar ratio of 1,3,5,7-tetrahydroxyadamantane and polycaprolactone. Moreover, the incorporation of 1,3,5,7-tetrahydroxyadamantane substantially improved the mechanical properties of the final polyurethane networks. The versatility of the synthesized polyurethane networks is reflected by its dual- and triple-shape memory performance. It can be anticipated that the synthesized polyurethane networks could be used as smart materials for applications involving shape memory polymers.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-7
·
IF 5.705
Liu Yufei,Yan Huijuan,Shangguan Jingfang,Yang Xue,Wang Meili,Liu Wei
A fluorometric aptamer-based assay for ochratoxin A (OTA) is described. It is making use of magnetic separation and a cationic conjugated fluorescent polymer. Amino-tagged aptamer (Apt) against OTA is immobilized on magnetic beads (MBs) to form a conjugate of type Apt-MBs. The immobilized aptamer is partially complementary to carboxyfluorescein-labeled DNA which binds to the Apt-MBs via hybridization if OTA is absent. Only few FAM-DNA will remain in the supernatant after magnetic separation, and only weak fluorescence resonance energy transfer (FRET) occurs on addition of the fluorescent polymer. If, however, OTA is present, it will bind to the aptamer and prevent the hybridization between Apt-DNA and FAM-DNA. This results in the presence of large amounts of FAM-DNA in the supernatant after magnetic separation. On addition of fluorescent polymer, efficient FRET occurs from the polymer to FAM-DNA. Fluorescence, best measured at excitation/emission peaks of 370/530 nm, increases with increasing concentrations of OTA. This assay is highly sensitive and selective. The detection limit is as low as 0.11 ng mL −1 . This is 6 times lower than the aptamer assay without using the fluorescent polymer. Conceivably, this method has a wider scope in that it may be extended to other mycotoxins by simply changing the aptamer.
Schematic of a fluorometric aptamer assay for ochratoxin A (OTA). It is based on magnetic separation coupled with a cationic conjugated polymer (PFP).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2018
·Vol. 29
·期 7
·1-13
·
IF 2.448
Song Xi,Cui Siyuan,Li Zhentao,Jiao Yanpeng,Zhou Changren
Chitin/graphene oxide (Ch/GO) composite sponges had been synthesized in 11 wt% NaOH/4 wt% urea aqueous solution by a simple method. The structure, thermal stability and mechanical properties of the composite sponges were investigated by scanning electron microscopy, Fourier-transform infrared spectroscopy, wide-angle X-ray diffraction, thermogravimetric analysis, and compressive strength measurements. The results revealed that chitin and GO were mixed homogeneously. Interestingly, the composite sponges showed meso-macroporous structure, which played an important role in improving their adsorption properties. Besides, thermal stability and mechanical properties were significantly improved compared with pure chitin sponges. Taking advantages of these fantastic characteristics, the maximum adsorption capacity of composite sponges for bilirubin was up to 422.9 mg/g under the optimized condition, which was not only significantly higher than the adsorption capacities of pure chitin sponges, but also superior to those of many reported adsorbents for removal of bilirubin. Furthermore, blood compatibility evaluations confirmed that this blended sponges had negligible hemolysis and coagulation. Therefore, this work provided a potential possibility to offer Ch/GO composite sponges for removal of bilirubin.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC Nephrology
·
2018
·Vol. 19
·期 1
·1-12
·
IF 2.395
Yang Huan,Male Musa,Li Yang,Wang Ning,Zhao Chenming,Jin Shan,Hu Juncheng,Chen Zhiqiang,Ye Zhangqun,Xu Hua
Background Substrate reduction therapy with analogs reduces the accumulation of substrates by inhibiting the metabolic pathways involved in their biosynthesis, providing new treatment options for patients with primary hyperoxalurias (PHs) that often progress to end-stage renal disease (ESRD). This research aims to evaluate the inhibition efficacy of Hydroxy-L-proline (HYP) analogs against calcium oxalate (CaOx) crystal formation in the Drosophila Melanogaster ( D. Melanogaster ) by comparing them with Pyridoxine (Vitamin B6). Methods Three stocks of Drosophila Melanogaster (W 118 , CG3926 RNAi, and Act5C-GAL4/CyO) were utilized. Two stocks (CG3926 RNAi and Act5C-GAL4 /CyO) were crossed to generate the Act5C > dAGXT RNAi recombinant line (F 1 generation) of D. Melanogaster which was used to compare the efficacy of Hydroxy-L-proline (HYP) analogs inhibiting CaOx crystal formation with Vitamin B 6 as the traditional therapy for primary hyperoxaluria. Results Nephrolithiasis model was successfully constructed by downregulating the function of the dAGXT gene in D. Melanogaster ( P -Value = 0.0045) . Furthermore, the efficacy of Hydroxy-L-proline (HYP) analogs against CaOx crystal formation was demonstrated in vivo using D. Melanogaster model; the results showed that these L-Proline analogs were better in inhibiting stone formation at very low concentrations than Vitamin B 6 (IC 50 = 0.6 and 1.8% for standard and dietary salt growth medium respectively) compared to N-acetyl-L-Hydroxyproline (IC 50 = 0.1% for both standard and dietary salt growth medium) and Baclofen (IC 50 = 0.06 and 0.1% for standard and dietary salt growth medium respectively). Analysis of variance (ANOVA) also showed that Hydroxy-L-proline (HYP) analogs were better alternatives for CaOx inhibition at very low concentration especially when both genetics and environmental factors are intertwined ( p < 0.0008) for the dietary salt growth medium and ( P < 0.063) for standard growth medium. Conclusion Addition of Hydroxy-L-Proline analogs to growth medium resulted in the reduction of CaOx crystals formation. These analogs show promise as potential inhibitors for oxalate reduction in Primary Hyperoxaluria.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 7
·1-8
·
IF 5.705
Wen Haibin,Jiang Peichun,Hu Yuling,Li Gongke
Poly(3,4-dihydroxy-L-phenylalanine) (polyDOPA) is a stable and biocompatible reducing agent. A versatile strategy is described here for the synthesis of core-shell Au@Ag nanostructures containing a polyDOPA interlayer. The latter provides abundant sites for deposition of nanocomposites, to immobilize molecules and to grow shells. The Au@polyDOPA@Ag nanoparticles are shown to generate strong and stable surface-enhanced Raman spectroscopy (SERS) signals compared to bare AuNPs and bare AgNPs. Folic acid was then immobilized on Au@polyDOPA@Ag nanoparticles and then applied to SERS imaging of human lung adenocarcinoma cell line A549 by the specific recognition of the folic acid receptor. The folic acid-conjugated SERS tags were promising to be nanoplatforms for imaging of cancer cells.
An Au@Ag core-shell nanostructures SERS nanotag with a polyDOPA interlayer was fabricated and then applied to SERS imaging of epithelial cells. (DOPA: 3,4-Dihydroxy-[ L -phenylalanine]; FA: folic acid; 4-MBA: 4-mercaptobenzoic acid).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 25
·6643-6651
·
IF 3.307
Chai Mengsa,Chen Yihui,Xuan Rongrong,Ma Junfeng,Jin Zhenfeng,Wang Tingting,Qiu Dan,Zhang Lihua,Zhang Yukui
A polyethyleneimine (PEI)-modified attapulgite was employed as a new adsorbent for solid-phase extraction (SPE) of chlorophenols (CPs) from environmental water samples. Key factors pivotal to extraction efficiency, such as organic additive, pH, salt, sample loading volume, elution volume, and sample loading flow rate, were investigated. The maximum adsorption capacity of CPs reached 38 mg/g, and the adsorption behavior could be described with the Langmuir isotherm model. The developed SPE procedure was then tested on river water samples. Of this cartridge, 0.4 g could be used to treat up to 100 mL of the water sample, with high recoveries achieved. The limit of detection ( S / N = 3) and the limit of quantification ( S / N = 10) were in range of 0.08–0.56 and 0.27–1.88 ng/mL, respectively. The mean recoveries of CPs spiked in river water samples ranged from 84.4 to 96.8% with relative standard deviations for the intra-day and inter-day less than 6.30%. The developed SPE method exhibited high sensitivity, high selectivity, excellent accuracy, and good repeatability to the analysis of trace CPs in complicated aqueous matrices.
ᅟ
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2018
·Vol. 29
·期 17
·14803-14812
·
IF 2.324
Zhang Kun,Li Na,Wang Yi,Ma Xiaoxuan,Zhao Jiupeng,Qiang Liangsheng,Hou Shuai,Ji Junyi,Li Yao
A improved bifunctional nanostructure composite was designed and fabricated as reversible electrodes for electrochromic film and lithium-ion battery. This unique optic-electro responsive urchin-like architecture assembled from WO 3 @PANI nanobelts were prepared by solvothermal and electropolymerization methods. As expected, the composite exhibits the superior optical-electrochemical performances. The composite owns quick switching time ranging from purple, green, yellow, gray to blue via voltage regulation, better rate performance and long-term cycling stability in the galvanostatic charge/discharge process. Benefited from stable structure and short diffusion path, it also demonstrates a distinct optical modulation (△T = 45%) and excellent durability after long-term cycles (1200 cycles). The as-prepared electrode exhibits outstanding cycling stability (capacity retention of 516 mAh g −1 after 1200 cycles with a low average fading capacity of ca. 0.103 mAh g −1 and fading cyclic rate of ca. 0.02% per cycle). The long-term stability studies reveal that urchin-like composite has much more excellent optical and electrochemical durability. The morphology and structure of the composite were carried out by characterization equipment. This effective synthesis strategy will have profound implications for developing the other inorganic–organic nanocomposites in optical and electrochemical field.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2018
·Vol. 47
·期 10
·5993-5999
·
IF 1.566
Sun Shiqing,Jiang Guohua,Liu Yongkun,Yu Bo,Evariste Uwamahoro
Supercapacitors (SCs) have drawn considerable attention as one of the energy storage devices. The key to fabricating flexible supercapacitors lies in the acquirement of flexible electrodes. In this study, the manganese oxide (MnO 2 ) and reduced graphene oxide (RGO) hybrid films (MnO 2 /RGO) were prepared by a filtration deposition and thermal reduction technique. Electrochemical measurements show that the hybrid films exhibit a specific capacitance of 333.9 F g −1 at 0.5 A g −1 , and 87% capacitance after 3000 cycles at 0.5 A g −1 . The flexible symmetric SCs was further fabricated with two pieces of MnO 2 /RGO hybrid films as electrodes. The fabricated flexible and sandwich type symmetrical SCs exhibited a safe working range with a potential window of 0–0.7 V with a maximum energy density of 23.5 Wh kg −1 at 0.5 A g −1 and the maximum power density of 1716.9 W kg −1 at 2.25 A g −1 .
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）