Biomaterials Science
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2022
·Vol. 10
·期 18
·5318-5325
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IF 7.59
Lina Dong,Yingzi Ren,Wei Zhang,Yu Liu,Mingzhuo Liu,Can Hong,ManYu Wang,Bowen Zhan,Xingwei Ding,Xiaolei Wang
Pursuing painless and flexible blood glucose regulation has been a century-long arduous mission. The current therapeutic systems can only regulate blood glucose unidirectionally (reduce), and the adjustment range is large, which is prone to the risk of hypoglycemia. Herein, inspired by the temperature fluctuation range controlled by the inverter air conditioner, we report a new bi-directional blood glucose-regulating drug delivery system (BDRS) consisting of glucose-loaded pressure-responsive nano-vesicles (Glu@PRNV), insulin-loaded black phosphorus nanosheets (Insulin@BPNs), hydrogel, and a painless blood sugar monitor patch. At first, BDRS could monitor blood glucose in real-time through visible color changes. Afterward, according to different requirements, BDRS could release glucose with the guidance of external pressure, or supplement insulin under near-infrared (NIR) irradiation, through which, the blood glucose level of diabetics could be accurately accommodated within a reasonable fluctuation range, thus minifying the likelihood of sudden hyperglycemia or hypoglycemia. Collectively, the supply–demand balance of blood glucose could be maintained via this real-time bi-directional drug delivery system, thereby improving the quality of life of diabetics. We have also verified the universality of this technique through a similar bi-directional sleep regulation.
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International Journal of Nanomedicine
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2022
·
IF 7.033
Ju Liang,Runfa Guo,Maosong Xuan,Qiankun Sun,Wenlan Wu
Purpose This study aimed to construct a DOX conjugate with liver tumor targeting and acid sensitivity based on a short aromatic peptide FFYEE, which could amplify the tumor inhibition efficacy of DOX and alleviate tissue toxicity. Methods A novel DOX-peptide conjugate, D-gal-FFYEE-hyd-DOX, was constructed by linking DOX to the side chain of FFYEE with acid-sensitive hydrazone bond and by modifying the C-terminal of peptide with α-D-galactosamine (D-gal) as targeting ligand. The structure of D-gal-FFYEE-hyd-DOX was characterized by mass spectrometry, infrared spectroscopy (IR), and UV-Vis spectroscopy (UV-Vis). The assembly characteristics of pentapeptide FFYEE and D-gal-FFYEE-hyd-DOX were observed by transmission electron microscope (TEM). In vitro drug release, cytotoxicity, endocytosis, in vivo antitumor experiment and histopathology analysis were investigated. Results Peptide FFYEE endowed the D-gal-FFYEE-hyd-DOX with self-assembly performance and improved biocompatibility. D-gal-FFYEE-hyd-DOX can self-assemble into nanofibers with a diameter of ~ 40 nm in neutral aqueous solution and significantly reduced the cytotoxicity of free DOX to L02 cells. In vitro drug release results showed that D-gal-FFYEE-hyd-DOX had acid sensitivity and controlled release characteristics. The cytotoxicity and endocytosis investigations confirmed that D-gal-FFYEE-hyd-DOX enhanced the cellular uptake of DOX and inhibition effect on HepG2 cells. In vivo antitumor experiment indicated that D-gal-FFYEE-hyd-DOX could significantly inhibit the growth of liver tumor in mice and reduce the side effects of DOX. Conclusion The conjugate D-gal-FFYEE-hyd-DOX with liver tumor targeting and acid sensitivity has the characteristics of strong tumor inhibition and low toxicity, hinting the great clinical application potential for targeted delivery of DOX in cancer treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Zeng Wen,Die Gao,Jing Lin,Siyi Li,Kailian Zhang,Zhining Xia,Dandan Wang
Magnetic porous cellulose molecularly imprinted polymers-based bisphenols have been developed using Fe 3 O 4 as the magnetic material, a deep eutectic solvent as the assisted solvent, and N-isopropylacrylamide as the functional monomer. The resulting magnetic porous cellulose molecularly imprinted polymers were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometry, thermal gravimetric analysis, and Brunauer–Emmett–Teller analysis. Moreover, the adsorption properties of the magnetic porous cellulose molecularly imprinted polymers toward bisphenol A, bisphenol F, and bisphenol AF were investigated using static, dynamic, and selective adsorption experiments. The introduction of porous cellulose materials significantly improves the capabilities of the material. The adsorption capacity, mass transfer efficiency, and selectivity of the magnetic porous cellulose molecularly imprinted polymers toward bisphenol A were 5.9, 4.0, and 4.4 times those of traditional molecularly imprinted polymers. Moreover, the adsorption stability of the magnetic porous cellulose molecularly imprinted polymers was investigated under different temperature and pH conditions. The adsorption characteristics of the magnetic porous cellulose molecularly imprinted polymers toward the target molecules were investigated using adsorption isotherm, kinetic, and thermodynamic models. Hydrogen bonding is the main interaction formed between the magnetic porous cellulose molecularly imprinted polymers and the target molecules. Magnetic porous cellulose molecularly imprinted polymers have great application value with excellent stability and reusability. Finally, the combination of the magnetic porous cellulose molecularly imprinted polymers and high-performance liquid chromatography or ultra-performance liquid chromatography-mass spectrometry was successfully used for the purification and detection of bisphenols in milk (1.349 ng/mL bisphenol F and 3.014 ng/mL bisphenol AF), canned fruits (1129 ng/mL bisphenol A, 10.11 ng/mL bisphenol F, and 91.87 ng/mL bisphenol AF), and fish (11.91 ng/mL bisphenol AF) samples. Furthermore, the magnetic porous cellulose molecularly imprinted polymer method is more selective, sensitive, and accurate than the traditional precipitation method.
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DYES AND PIGMENTS
·
2022
·
IF 5.122
Chengpei Du,Lin Qi,Yi Wang,Kanglin Pei,Renwen Zhang,Di Wu,Wenjing Qi
A fluorescent dual “turn-on” method is utilized to detect acid phosphatase (ACP) via dual quenching and fluorescence resonance energy transfer (FRET) strategy to improve sensitivity for the first time. Bovine serum albumin protected gold nanoclusters (BSA-AuNCs) are utilized as fluorescence probe and peroxidase-mimicking catalyst. BSA-AuNCs oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to TMB (oxTMB) with the absorbance peak at 652 nm. Fluorescence spectrum of BSA-AuNCs with emission peak at 635 nm is largely overlapped with absorption spectrum of oxTMB, which brings FRET from BSA-AuNCs to oxTMB and fluorescence is quenched. When Ce 3+ is added, BSA-AuNCs catalyze the oxidation of Ce 3+ to Ce 4+ by H 2 O 2 . Ce 4+ has oxidizing properties, which can oxidize TMB into oxTMB and enhance FRET. It achieves the fluorescence is quenched further more. This dual fluorescence quenching effect brings a low background for ACP detection. ACP catalyzes ascorbic acid 2-phosphate (AA2P) hydrolysis into phosphate ion (PO 4 3− ) and ascorbic acid (AA). Owing to high coordination between Ce 3+ and PO 4 3− , TMB oxidation is prevented and fluorescent is turned-on. AA reduces oxTMB into TMB, which makes FRET be prevented and fluorescence is also increased. Therefore it is utilized in ACP detection from 0.01 to 2 U/L with high selectivity and accuracy.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2022
·Vol. 414
·期 22
·6441-6453
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IF 4.478
Li Guiyin,Chen Wei,Mi Danhong,Wang Bo,Li HaiMei,Wu Guangxiong,Ding Ping,Liang Jintao,Zhou Zhide
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths in China. Glypican-3 (GPC3) is a specific antigen related to HCC, which is widely used in clinical detection as a reliable marker of HCC. In this paper, a highly sensitive homogeneous apatasensor was designed for GPC3 detection based on fluorescence resonance energy transfer (FRET) where the GPC3 aptamer labelled gold carbon dots (AuCDs-GPC3 Apt ) are used as a donor and magnetic graphene oxide (Fe 3 O 4 /GO) nanosheets are used as an acceptor. A one-step hydrothermal method was used to synthesize AuCDs to provide sufficient fluorescence. The FRET phenomenon exists between AuCDs-GPC3 Apt and Fe 3 O 4 /GO, which weakens the fluorescence intensity of the whole system. When the target GPC3 is added to the FRET system, the fluorescent AuCDs-GPC3 Apt binds to the GPC3 and forms a folded structure, which leads to AuCDs-GPC3 Apt separation from Fe 3 O 4 /GO nanosheets. The Fe 3 O 4 /GO is then magnetically separated so that the fluorescence of free labelled AuCDs-GPC3 Apt is restored. Under the optimum conditions, the fluorescence recovery rate is linearly correlated with the concentration of GPC3 (5–100 ng·mL −1 ) and the detection limit is 3.01 ng·mL −1 (S/N = 3). This strategy shows recoveries from 98.76 to 101.29% in real human serum samples and provides an immediate and effective detection method for the quantification of GPC3 with great potential applications for early diagnosis of HCC. Graphical abstract A sensitive homogeneous FRET-based apatasensor was designed for GPC3 detection where the AuCDs-GPC3 Apt is a donor and Fe 3 O 4 /GO nanosheets are an acceptor. The GPC3 fluorescent aptasensor combines wider output range with low cost, high specificity, and good anti-interference.
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PROGRESS IN ORGANIC COATINGS
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2022
·
IF 6.206
Tongtong Ding,Luyao Xu,Xiangting Liu,Liang Ma,Yingxin Cui,Dalong Li,Xiuhua Sun,Changlu Gao
To achieve prolonged and sustainable release of copper ion in Cu 2 O-containing marine coatings, poly(lauryl methacrylate)-b-poly(2-( N , N -dimethylamino)ethyl methacrylate) (PLMA-b-PDMAEMA) diblock copolymers with different block ratios and molecular weights were synthesized by atom-transfer radical polymerization (ATRP) method. It was proved that the copolymers are able to coordinate with copper ion. And the surface wettability tests showed that the amphiphilic copolymers may undergo structure change in seawater. Taking advantage of the structure transformation and copper coordination abilities, the copolymer was employed to encapsulate Cu 2 O for the preparation of antifouling coatings. A controllable and continuous release of copper ion from Cu 2 O/copolymer coatings was then obtained. More importantly, it showed that higher PLMA/PDMAEMA block ratio exhibited better bactericidal rate. Actually, the bactericidal ability of the coating depends on the time duration of copper ion on the surface instead of the hydrolysis speed of the coating, in other words, the hydrophobic interaction of PLMA with the resin may play a major role in the antifouling performance of the marine coating. Self-polishing coatings Cu 2 O/copolymer/polycaprolactone (PCL) were then prepared and applied for marine field test, the antifouling performances were proved to be comparable to that of the commercially available self-polishing coatings.
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Journal of Environmental Chemical Engineering
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2022
·
IF 7.968
Hongji Li,Dandan Wang,Chun Miao,Fengwu xia,Yubo Wang,Yutong Wang,Chunbo Liu,Guangbo Che
Photo-degradation of pollutant and photo-reduction of CO 2 are efficient ways to address challenges of water pollution , the greenhouse effect and energy crisis, respectively. In our work, via fastening two-dimensional (2D) BiOI nanoplates on 2D g-C 3 N 4 nanosheets , a BiOI/g-C 3 N 4 (BI/CN) S-scheme photocatalyst with closely stacked structure were prepared though a self-assembly process. The prepared BI/CN S-scheme heterojunction could improve the transfer and separation efficiency of photo-generated electron-hole pairs by facilitating the electrons transfer from BiOI to g-C 3 N 4 . Under visible light exposure, the photo-degradation efficiencies of tetracycline hydrochloride (TC) and p-chlorophenol (4-PC) by BI/CN-50% reached nearly 100% and 46%, respectively, which were more excellent in comparison with other samples. And BI/CN-50% sample exhibited the best CO production (12.45 μmol∙g −1 ) with visible-light irradiation of 4 h. This value was approximately 4.37 and 5.41 times higher than that of BI (2.85 μmol∙g −1 ) and CN materials (2.30 μmol∙g −1 ), respectively. Moreover, the excellent photocatalytic performance had no visible decay during five cycles. Meanwhile, an S-scheme charge transfer process was confirmed by species trapping experiments and electron spin-trap analysis. This study offers new insights into the activity, kinetics, and mechanism over BiOI-based materials for photocatalysis technology .
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COMPOSITES PART B-ENGINEERING
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2022
·
IF 11.322
Qian Wang,Weiwang Qiu,Hao Liu,Xiaoran Li,Xiaohong Qin,Xueli Wang,Jianyong Yu,Bin Li,Faxue Li,Liqian Huang,Dequn Wu
Diabetic chronic wounds have serious threats to public health, posing enormous challenges and financial burdens around the world. Monitoring and treatment of chronic diabetic wounds is an urgent issue that must be addressed right now. Herein, conductive hydrogels with multifunctionality were proposed and created by incorporating polydopamine (PDA) nanoparticles , glucose oxidase (GO x ) and catalase (CAT) into polydopamine/acrylamide (PDA/AM) hydrogels to monitor and repair diabetic chronic wounds. Under the irradiation of 808 nm Near-infrared (NIR), PDA nanoparticles encapsulated in PDA/AM hydrogels possessed good antibacterial capabilities (99.99%). The introduction of GO x not only lowered hyperglycemia to normal level (5.1 mmol/L), but it also improved wound hemostasis adhesion (38 kPa). Hydrogen peroxide (H 2 O 2 ) generated oxygen (O 2 , 18 mg/mL) via a cascade reaction under the catalysis of CAT, which could accelerate angiogenesis and stimulate cell migration and proliferation. The addition of ions enabled the hydrogel to have excellent electrical conductivity , allowing for precise wound healing monitoring. Conductive hydrogels with the multifunction of hemostasis, antibacterial, hyperglycemia manipulation, and O 2 generation show promise in diabetic foot wound repair and wound monitoring.
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PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2022
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IF 3.141
Zhen Zhang,Shancai Guo,Chuanding Chen,Jianyuan Lin,Leyuan Chen,Danni Wang,Jinjing Hu,Xinrui Chen,Jingyi Yang,Yingying Li,Xinxuan Wang
In the present study, ultrasound-assisted extraction was employed to extract the general flavone from celery leaves using response surface methodology and BP neural network model with a genetic algorithm (GA). The effects of temperature, time, solid-liquid ratio, and ethanol concentration on the extraction results were assessed by Box-Behnken design. Further optimization of the process was performed by GA-BP. Our results showed that the optimal conditions were an ethanol concentration of 70.31%, a temperature of 67.2 °C and an extraction time of 26.6 min. In addition, significant antioxidant activity and in vitro bacteriostasis were observed. We found that the total flavonoids of the celery leaves exerted a strong inhibitory effect on Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. Additionally, considerable DPPH· and ·OH scavenging effects were exerted by flavonoids. Therefore, flavonoids from celery leaves can be considered natural antioxidants and bacterial inhibitors.
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Frontiers in Bioengineering and Biotechnology
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2022
·
IF 6.064
Jiang Mingyue,Chen Le,Chen Bo,Yu Qinghua,Zhang Xianming,Jing Weihong,Ma Limei,Deng Tao,Yang Zhangyou,Yu Chao
Curcumin (CUR) is a natural bioactive compound that has attracted attention as a “golden molecule” due to its therapeutic properties against several types of tumors. Nonetheless, the antitumor application of CUR is hampered due to its extremely low aqueous solubility and chemical instability. Herein, a novel type of CUR-loaded polymeric micelles with intracellular K+-responsive controlled-release properties is designed and developed. The polymeric micelles are self-assembled by poly (N-isopropylacrylamide-co-acryloylamidobenzo-15-crown-5-co-N, N-dimethylacrylamide)-b-DSPE (PNDB-b-DSPE) block copolymers, and CUR. CUR is successfully loaded into the micelles with a CUR loading content of 6.26 wt%. The proposed CUR-PNDB-DSPE polymeric micelles exhibit a significant CUR release in simulated intracellular fluid due to the formation of 2 : 1 ‘‘sandwich’’ host–guest complexes of 15-crown-5 and K+, which lead to the hydrophilic outer shell of micelles to collapse and the drug to rapidly migrate out of the micelles. In vitro, the B16F10 cell experiment indicates that CUR-PNDB-DSPE micelles exhibit a high cellular uptake and excellent intracellular drug release in response to the intracellular K+ concentration. Moreover, CUR-PNDB-DSPE micelles show high cytotoxicity to B16F10 cells compared to free CUR and CUR-PEG-DSPE micelles. The polymeric micelles with intracellular K+-responsive controlled release properties proposed in this study provide a new strategy for designing novel targeted drug delivery systems for CUR delivery for cancer treatment.
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DYES AND PIGMENTS
·
2022
·
IF 5.122
Yingying Xiang,Yujiao Tu,Lei Jiang,Lin Yuan,Ze Liu,Qiang Xie,Xuequan Xiong,Fei Song
As one of the most widely used azo synthetic dyes, long-term consumption of excessive amaranthcan cause a threat to health. It is very urgent to establish high selective and sensitive method for amaranth detection. Orange fluorescent carbon dots utilizing l -methionine and o-phenylenediamine as raw materials, namely S,N-CDs, were prepared via one-step hydrothermal method . Dialysis, thin layer chromatography and chromatographic column were employed for further purification. The purified dual-mode nanoprobe based on fluorescent and UV absorption was successfully established. For fluorescent, excellent linear ranges of 5–70 μM and 110–195 μM were obtained, and low detection limit was 345.22 nM. For UV absorption, excellent linear ranges of 0–155 μM with low detection limit 12.70 nM. It revealed that inner filter effect and static quenching dominated in amaranth quenching the fluorescent of S,N-CDs. Furthermore, the dual-mode nanoprobe could be efficiently applied to determine amaranth in fruit juice, carbonated drink, and strawberry jam samples. It provided a new insight on the purification of carbon dots and detection of amaranth by dual-mode nanoprobe.
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MACROMOLECULAR MATERIALS AND ENGINEERING
·
2022
·Vol. 307
·期 10
·2200262
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IF 4.402
Zhiwen Zeng,Cuiping Guo,Daohuan Lu,Zhijie Geng,Dating Pei,Shan Yu
Infection and chronic inflammation caused by oxidative stress are the main challenges in chronic wound healing. In this study, a silk fibroin/kappa-carrageenan ( k CA)-based hydrogel functionalized with epigallocatechin-3-gallate (EGCG) and Cu 2+ is fabricated for chronic wound healing. Polyphenol–metal functional complexes and ionic-covalent entanglement networks are prepared by introducing Cu 2+ . Cu 2+ coordinates with k CA to enhance the mechanical properties and chelates with EGCG to form an EGCG-Cu 2+ complex that continuously releases EGCG. The sustained release of EGCG enhances the antibacterial properties by synergizing with the effects of Cu 2+ . Furthermore, the prepared hydrogel exhibits a potent ability to scavenge excessive intracellular reactive oxygen species, controllable antioxidant bioactivity, and reliable biocompatibility. Ultimately, the resulting multifunctional hydrogel dressing provides a potential and effective strategy for chronic wound care management with increased mechanical strength and antibacterial and antioxidant properties.
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