ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Mingchuan Yu,Di Wang,Da Zhong,Weichang Xie,Jun Luo
Diabetic kidney disease (DKD) is a common diabetes complication mainly caused by lipid toxicity characterized by oxidative stress. Studies have shown that adropin (Ad) regulates energy metabolism and may be an effective target to improve DKD. This study investigated the effect of exogenous Ad encapsulated in reactive oxygen species (ROS)-responsive nanocapsules (Ad@Gel) on DKD. HK2 cells were induced with high glucose (HG) and intervened with Ad@Gel. A diabetes mouse model was established using HG and high-fat diet combined with streptozotocin and treated with Ad@Gel to observe its effects on renal function, pathological damage, lipid metabolism, and oxidative stress. Results showed that Ad@Gel could protect HK2 from HG stimulation in vitro. It also effectively controls blood glucose and lipid levels, improves renal function, inhibits excessive production of ROS, protects mitochondria from damage, improves lipid deposition in renal tissues, and downregulates the expression of lipogenic proteins SEBP-1 and ADRP in DKD mice. In HG-induced HK2 cells or the kidney of DKD patients, the low expression of neuronatin (Nnat) and high expression of translocator protein (TSPO) were observed. Knockdown Nnat or overexpression of TSPO significantly reversed the effect of Ad@Gel on improving mitochondrial damage. In addition, knockdown Nnat also significantly reversed the effect of Ad@Gel on lipid metabolism. The results suggest that the effect of Ad on DKD may be achieved by activating Nnat to improve lipid metabolism and inhibit TSPO activity, thereby enhancing mitochondrial function.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Mengen Dang,Dandan Chen,Ping Lu,Guiling Xu
Poplar wood (PW) hydrochar modified by iron (Fe@HC) was prepared greenly by one-step hydrothermal method. The adsorption and degradation performance of DDT was investigated in a heterogeneous advanced oxidation system (Fe@HC/PMS) formed by Fe@HC collaborated with peroxymonosulfate (PMS). The effects of Fe@HC dosage, PMS dosage and DDT initial concentration were quantitatively analyzed. The results showed that DDT removal efficiency can reach to 88.62% in 240 min under optimal conditions (4 g/L Fe@HC, 10 mM PMS, 0.5 mg/L DDT, 5.5 pH 0 ) in Fe@HC/PMS system. Furthermore, Fe@HC/PMS system exhibited high degradation rate and TOC removal efficiency for the removal of various organic contaminants . The influence mechanisms of Fe@HC/PMS system on DDT adsorption and degradation were proposed based on electron paramagnetic resonance (EPR) testing analysis and radical quenching experiments. Based on the mechanism analysis, the influence of Fe@HC/PMS on DDT removal efficiency can be concluded in the order: Active substance indirect degradation (60.95%) > Fe@HC direct degradation (10.13%) > Fe@HC adsorption (17.54%). Among active substance indirect degradation, SO 4 •− , • OH, O 2 •− and 1 O 2 occupied 27.56%, 15.74%, 5.33% and 12.32%, respectively. Moreover, DDT degradation intermediates were detected by a gas chromatography-mass spectrometer (GC-MS) to predict DDT degradation pathways. This study provided a green progress for the reuse of biomass resources and a new way for the enhanced degradation of DDT.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Yi Liang,Peng Zhao,Jilin Zheng,Yuanyuan Chen,Yiyi Liu,Jia Zheng,Xiaogang Luo,Danqun Huo,Changjun Hou
Reasonable sensor construction for precisely real-time detecting H2O2 produced from living cells has always been of great significance and challenges. Due to the excellent catalytic efficiency and unparalleled peroxidase-like activity, single-atomic catalysts with Fe-Nx active sites show great potential in H2O2 detection. These special atomic active sites make it easier for the Fenton reaction to occur, which provides basic support for H2O2 sensors. Their application in the in situ real-time detection of H2O2 has important market prospects. Herein, single-atomic Fe catalysts with distorted graphitic carbon are obtained through a simpler preparation method. A precursor hemin@zeolitic imidazolate framework-8 (hemin@ZIF-8) is calcined at a high temperature and carbonized to get the nitrogen-carbon codoped Fe single atoms (Fe SAs-N/C) and then successfully prepare a H2O2 electrochemical sensor by modifying it on a glassy carbon electrode (GCE). Its peroxidase activity is calculated by enzymatic kinetics, which shows a better peroxidase activity compared with horseradish peroxidase and other reported single-atom (SA) materials. Moreover, the electrocatalytic kinetics is explained by Laviron calculation, which more directly confirms that it has a faster electron transfer rate. Importantly, Fe SAs-N/C@GCE has a wider linear range than sensors combined with other SA materials and a low detection limit of 0.34 μM. It is an attractive design of H2O2 sensors and single-atomic catalysts with superior substrate affinity that are novel alternatives to natural enzymes.
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2022
·
IF 6.443
Youjiao Wu,Lili He,Yi Yang,Zhigang Yan,Zhifeng Zhang,Xiaojun Yao,Pei Luo
Smilax glabra Roxb. (SG) is widely used as functional food with various beneficial effects. Fresh SG without processing has been eaten directly for anti-inflammation from ancient China, while the underlying mechanism remains underexplored. This study aims to investigate the anti-inflammatory activity of fresh SG by using metabolites profiles, affinity ultrafiltration mass spectrometry, PDE4 enzyme inhibition assay, and in silico analysis. Encouragingly, fresh SG showed promising anti-inflammatory effect with IC 50 value (0.009 μg/μL) on PDE4 was about 12 times higher than that of processed SG (0.110 μg/μL). Astilbin was identified as the main bioactive compound of fresh SG responsible for PDE4 inhibitory activity. We found that heat processing strongly affected astilbin isomerization, leading to significant changes in contents and PDE4 inhibitory activities of four astilbin isomers, resulting in decreased anti-inflammatory activity of fresh SG. This finding will provide theoretical basis for systematic research and food/nutraceutical applications of fresh Smilax glabra in the future.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Zhang Hongzhen,Zhang Chenghua,Xiang Xiaolong,Zhang Qilun,Zhao Wei,Wei Guoyu,Hu Anlong
Kasugamycin (KSM), an aminoglycoside antibiotic, has been widely used for the management of plant diseases, especially for the control of rice blast in Asia. However, its uptake mechanism and transport in plants are still obscure. The castor bean (Ricinus communis L.) seeding, a model plant for phloem transport, was used to study the mechanism of uptake and transport of KSM. Results showed that cotyledon-applied KSM could transport into the phloem and distributed in root and shoot of plant. The temperature, concentration, and pH had significant effects on the uptake of KSM, indicating that the uptake of KSM was mediated by an active carrier system. Compared with the control, competitive inhibitors of sugar transporters D-glucose, D-chiro-inositol, and phloridzin inhibited 71.03%, 67.95%, and 61.73% uptake of KSM, respectively. Energy inhibitor dinitrophenol (DNP) and carbonyl cyanide chlorophenylhydrazone (CCCP) also affected the uptake of KSM, and the inhibition rates were 34.23% and 48.06%. All the results showed that the uptake of KSM was mediated by a sugar transporter, and it could transport from shoot to root in plants via the phloem. The study preliminary elucidated the plant–microbe interactions in the context of the transport of microbial secondary metabolites in plants. It has certain significance for scientific application of antibiotics and biological control of plant diseases and provides theoretical basis for the development of bidirectional transport pesticides.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2022
·Vol. 13
·期 1
·1-13
·
IF 17.694
Xiao Kui,Zhang Niyuan,Li Feifei,Hou Dayong,Zhai Xiaoyi,Xu Wanhai,Wang Gelin,Wang Hao,Zhao Liang
Medicinal applications of gold complexes have recently attracted attention due to their innovative antitumor mechanisms. In this work, two hypercoordinated carbon-centered gold clusters PAA4 and PAA5 are quantitatively synthesized by an intramolecular 6- exo - dig cyclization of polymetalated precursors. The on-bench and in vitro experimental studies demonstrate that the characteristic hypercarbon-tetragold(I) multi-center bonding in PAA4 and PAA5 not only guarantees their stability under common physiological conditions, but also facilitates a glutathione (GSH)-triggered prompt and synergetic release of active Au(I) ions in the GSH-overexpressed and acidic microenvironment of human bladder cancer EJ cells. The instantly massive release of coordination unsaturated Au(I) ions causes the efficient inhibition of thioredoxin reductases and then induces a rapid pro-oxidant response, consequently causing the occurrence of accelerated ferroptosis of EJ cells. As a result, these hypercarbon-centered gold(I) cluster prodrugs show high cytotoxicity to bladder cancer cell lines and thus exhibit a significant inhibition effect towards bladder tumors in vivo. Correlation of the synergetic domino dissociation of carbon-polymetal multi-center bonding in metal clusters with the accelerated ferroptosis of cancer cells provides a strategy for metallo-prodrugs and opens a broader prospect for the biological application of metal cluster compounds.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Wenjie Feng,Wanrui Shi,Ze Wang,Yanqi Cui,Xinxin Shao,Shuwei Liu,Li Rong,Yi Liu,Hao Zhang
Ferroptosis has been considered as a promising pathway to overcome apoptosis-induced tumor chemoresistance. However, the antitumor efficacy of ferroptosis-inducing agents is still limited because of the complexity and diversity of tumor microenvironments. Herein, we demonstrate a triple ferroptosis amplification strategy for tumor therapy by associating iron-based nanocarriers, ferroptosis molecular drugs, and H2O2-producing enzymes. Fe(III)-Shikonin (FeShik) metal-polyphenol-coordinated networks are employed to load a ferroptosis inducer of sorafenib (SRF) inside and glucose oxidase (GOx) outside, thus producing SRF@FeShik-GOx supramolecular nanomedicines (SNs). After delivering into glutathione (GSH)-overexpressed tumor cells, FeShik will disassemble and release Fe2+ to induce cell death via ferroptosis. At the same time, GOx executes its catalytic activity to produce an acid environment and plenty of H2O2 for stimulating •OH generation via the Fenton reaction. Moreover, SRF will suppress the biosynthesis of GSH by inhibiting system Xc–, further deactivating the enzymatic activity of glutathione peroxidase 4 (GPX4). Up-regulation of the oxidative stress level and down-regulation of GPX4 expression can dramatically accelerate the accumulation of lethal lipid peroxides, leading to ferroptosis amplification of tumor cells. The current strategy that utilizes ferroptosis-inducing agents as both nanocarriers and cargoes provides a pathway to enhance the efficacy of ferroptosis-based tumor therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2022
·Vol. 33
·期 10
·3718-3727
·
IF 3.348
Yang You,Anna Zheng,Dafu Wei,Xiang Xu,Yong Guan,Jianding Chen
As an essential elastomer used in edge technologies, fluorosilicone rubber (FSR) suffers serious oxidative aging problem when serving at high temperature. In this study, a small amount of reduced graphene oxide (rGO) was added to FSR, and the resulting rGO/FSR compounds were vulcanized using 2,5-dimethyl-2,5-di(tert-butyl) peroxyhexane as the vulcanizer. The dispersion of rGO was investigated using scanning electron microscope. The mechanical properties and thermal-oxidative stability of the rGO/FSR vulcanizates were investigated via thermal aging test at 230°C for 72 h, thermogravimetric analysis (TGA), and isothermal degradation test at 280, 320, and 350°C. The results showed the small addition of rGO (0.5 wt%) significantly improved the thermo-oxidative stability of FSR. In particular, the FSR incorporating of 0.5 wt% of rGO could maintain more than 85% of tensile strength after aging at 230°C for 72 h. The FSR incorporating of 0.5 wt% of rGO can maintain more than 90% of initial weight after aging at 350°C for 12 h. The experiment of scavenging hydroxyl radical by rGO, and quantum chemistry calculation indicated rGO containing aryloxy radicals can scavenge the initial radicals during thermal degradation of FSR, which might be prime reason for the antioxidant properties of rGO.
✓ 已验证
产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2022
·
IF 4.966
Bin Liu,Danyang Fu,Hang Ning,Ming Tang,Hui Chen
Bark beetles rely on detoxifying enzymes to resist the defensive terpenoids of the host tree. Insect cytochrome P450 (CYPs) plays a key role in the detoxification of pesticides and plant allelochemicals . CYP6 family is unique to Insecta, and its biochemical function is basically related to the metabolism of exogenous substances. In this study, we sequenced and characterized the full-length cDNAs of two CYP6 genes from Chinese white pine beetle, Dendroctonus armandi . Spatiotemporal expression profiling revealed that the expression of CYP6CR2 and CYP6DE5 was higher in larval and adult stages of D. armandi than that in other developmental stages, and that two genes predominantly expressed in brain , midgut, fat body, Malpighian tubules or hemolymph. The expression of CYP6CR2 and CYP6DE5 was significantly induced after feeding on the phloem of Pinus armandii and exposure to six stimuli [(±)- α -pinene, (−)- α -pinene, (−)- β -pinene, (+)-3-carene, (±)-limonene and turpentine]. Importantly, silencing CYP6CR2 and CYP6DE5 separately could increase the sensitivity, led to a significant reduction of the activity of P450, resulting a significant increase in adult mortality after treatment with terpenoids. The comprehensive results of this study showed that in the process of host selection and colonization, the functions of CYPs were mainly to hydrolyze the chemical defense of the host and degrade odor molecules. These findings may help to develop new treatments to control this important pest.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2022
·
IF 5.546
Hang Hu,Defeng Xu
In the present work, methoxypoly(ethylene glycol)-polycaprolactone (mPEG-Hyd-PCL) and lactobionic acid-grafted poly(ethylene glycol)-polycaprolactone (LA-PEG-Hyd-PCL) mixed nanoparticles were developed for targeted delivery of dimethylcurcumin (DMC) and regorafenib (RGF) for combinational therapy of hepatocellular carcinoma. mPEG-Hyd-PCL and LA-PEG-Hyd-PCL were synthesized and characterized by 1 H nuclear magnetic resonance spectrometry, flourier transform infrared spectrometry, and gel permeation chromatography. The mixed nanoparticles of mPEG-Hyd-PCL and LA-PEG-Hyd-PCL (m/L) were prepared by a dialysis method, in which nile red was loaded to study the effect of the composition of the mixed nanoparticles on the cellular uptake. The results show that the mixed nanoparticles of mPEG-Hyd-PCL and LA-PEG-Hyd-PCL with a weight ratio of 1:0.2 (m/L(1:0.2)) exhibit the highest cellular uptake efficiency on HepG2 cells in the presence 10% fetal bovine serum. In addition, m/L(1:0.2) nanoparticles can be selectively taken up by HepG2 cells. DMC and RGF co-loaded m/L(1:0.2) nanoparticles (DMC/RGF@m/L(1:0.2)) were then prepared and evaluated regarding the in vitro drug release, cellular uptake, and in vitro antitumor effect . The results show that DMC/RGF@m/L(1:0.2) nanoparticles exhibit pH-sensitive drug release, enhanced cellular uptake amount and in vitro antitumor effect on HepG2 cells as compared to DMC/RGF@mPEG-Hyd-PCL and DMC/RGF@LA-PEG-Hyd-PCL nanoparticles. Although the in vitro antitumor effect of DMC/RGF@m/L(1:0.2) nanoparticles is still lower than DMC/RGF mixture, they might achieve selective cytotoxicity against hepatoma cells.
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Mengjie Pu,Daqi Ye,Jinquan Wan,Bentuo Xu,Wei Sun,Wei Li
A novel peroxymonosulfate (PMS) catalyst, zinc-based metal–organic framework electrospinning (ES) nanofibers membrane (short for ZIF-65/PAN) was firstly fabricated and used for the degradation of aqueous ciprofloxacin (CIP). Physicochemical properties, catalytic performance, stability, reusability, and the regeneration of ZIF-65/PAN was observed and conducted. Influence of various parameters including ZIF-65 loading content, reaction temperature, initial pH, and competitive organic molecules on CIP degradation was investigated. Reactive active species and degradation intermediates that involved in ZIF-65/PAN/PMS/CIP process were identified. Corresponding reaction mechanisms were speculated. The results indicate that the easy-separation ZIF-65/PAN nanofibers exhibit high efficiency for PMS activation, 89.2 % of CIP was degraded within 60 min, with up to 87.5 % of PMS utilization efficiency. ZIF-65/PAN was renewable through simple layer-by-layer self-assembly, thus shows good prospect in future applications. 26.4 % of surface Zn 2+ of ZIF-65/PAN exists in the form of + III oxidation state after activation, indicating the role of zinc catalytic site. Through activation, oxidative active species SO 4 ·− , ·OH, and 1 O 2 was generated, CIP was thus degraded through mainly-three pathways. This study provides novel ideas for the application of MOF-based membrane on PMS based advanced oxidation processes.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Electronic Materials
·
2022
·
IF 4.494
Qungui Wei,Dan Yang
Utilizing the synergetic effects of different-sized fillers to effectively construct a thermally conductive network in polymer-based composites is an important strategy to dissipate the heat produced by electronic equipment. In this work, a remarkable synergetic efficiency is achieved in silicone rubber (SR) composites loaded with boron nitride-polydopamine-silver (BN-PDA-Ag) and carbon nanotube-polydopamine-silver (CNT-PDA-Ag) systems by constructing a hybrid heat conductive network. Specifically, PDA decreases the interface thermal resistance by improving the compatibility between the fillers and the polymeric matrix, while Ag nanoparticles further enhance thermal conductivity (TC). Finally, the TC value for SR composites reaches 0.655 W/mK, 4.17 times that of SR matrix. Additionally, a high dielectric constant and a relatively low electrical conductivity are obtained for SR composites. Overall, this strategy offers a valuable basis to prepare thermal management materials for application in electronic components.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）