TALANTA
·
2016
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IF 4.035
Songqing Chen,Xingxiu Qin,Weixi Gu,Xiashi Zhu
Ionic liquids-β-cyclodextrin polymer (ILs-β-CDCP) was attached on Fe 3 O 4 nanoparticles to prepare magnetic solid phase extraction agent (Fe 3 O 4 @ILs-β-CDCP). The properties and morphology of Fe 3 O 4 @ILs-β-CDCP were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction(XRD), size distribution and magnetic analysis. A new method of magnetic solid phase extraction (MSPE) coupled to ICP-OES for the speciation of Mn(II)/Mn(VII) in water samples was established. The results showed that Mn(VII) and total manganese [Mn(II)+Mn(VII)] were quantitatively extracted after adjusting aqueous sample solution to pH 6.0 and 10.0, respectively. Mn(II) was calculated by subtraction of Mn(VII) from total manganese. Fe 3 O 4 @ILs-β-CDCP showed a higher adsorption capacity toward Mn(II) and Mn(VII). Several factors, such as the pH value, extraction temperature and sample volume, were optimized to achieve the best extraction efficiency. Moreover, the adsorption ability of Fe 3 O 4 @ILs-β-CDCP would not be significantly lower after reusing of 10 times. The accuracy of the developed method was confirmed by analyzing certified reference materials (GSB 07-1189-2000), and by spiking spring water, city water and lake water samples.
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Chinese Medicine
·
2024
·Vol. 19
·期 1
·1-17
·
IF 5.3
Gao Jiaming,Guo Hao,Li Junmei,Zhan Min,You Yue,Xin Gaojie,Liu Zixin,Fan Xiaodi,Gao Qinghe,Liu Jianxun,Zhang Yehao,Fu Jianhua
Background Buyang Huanwu Decoction (BYHWD) is a traditional Chinese medicine to treat the syndrome of qi deficiency and blood stasis. Platelets play an important role in regulating thrombus and inflammation after ischemic injury, studies have shown that BYHWD regulate myocardial fibrosis and exert anti-inflammatory effects through IL-17 and TLR4 pathways, but the mechanism of platelet activation by BYHWD in stable coronary heart disease is still unknown. In the present study, model of left anterior descending coronary artery ligation was applied to investigate the mechanisms of BYHWD on modulating platelets hyperreactivity and heart function after fibrosis of ischemic myocardial infarction (MI). Methods Myocardial infarction model was constructed by ligation of the left anterior descending coronary artery. The rats were randomly divided into five groups: sham, model, MI with aspirin (positive), MI with a low dosage of BYHWD (BYHWD-ld) and MI with a high dosage of BYHWD (BYHWD-hd) for 28 days. Results Coronary artery ligation prominently induced left ventricle dysfunction, increased cardiomyocyte fibrosis, which was accompanied by platelets with hyperreactivity, and high levels of inflammatory factors. BYHWD obviously reversed cardiac dysfunction and fibrosis, increased the thickness of the left ventricular wall, and inhibited aggregation ratio and CD62p expression. BYHWD restored the mitochondrial respiration of platelets after MI, concomitant with an increased telomere expression and decreased inflammation. According to the result of transcriptome sequencing, we found that 106 differentially expressed genes compared model with BYHWD treatment. Enrichment analysis screened out the Ras-related protein Rap-1 (Rap1) signaling pathway and platelet activation biological function. Quantitative real-time PCR and Western blotting were applied to found that BYHWD reduced the expression of Rap1/PI3K-Akt/Src-CDC42 genes and attenuated the overactivity of PI3 kinase/Rap1/integrin α(IIb)β(3) pathway. Conclusion BYHWD reduced inflammation and platelet activation via the PI3 kinase/Rap1/integrin α(IIb)β(3) pathway and improved heart function after MI. Graphical Abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shuting Cao,Hongzhen Liu,Ming Qin,Nannan Xu,Fuhao Liu,Yuetao Liu,Chuanhui Gao
Considering that fruits are vulnerable to damage and waste during stockpiling, transport and marketing. Given this, an innovative curcumin inclusion compound (Cur@β-CD) was devised in this study to introduce oil-soluble curcumin (Cur) into water-soluble polyvinyl alcohol (PVA) materials, thereby fabricating food packaging films endowed with excellent properties. DPPH test manifested that the oxidation resistance for PCOMC-Cur@β-CD film was 95 % above PVA material. It was ascribed to the fact that the Cur@β-CD elevated the water solubility of Cur while the increase of water solubility heightened the antioxidant effect for Cur in the film. Additionally, the chitosan (CS) was crosslinked with malic acid (MA), which elevated the barrier property of the film, reduced the amount of oxygen transmission and further retarded the oxidation reaction of the fruits for packaging. The antibacterial test demonstrated that the antibacterial rates of PCOMC-Cur@β-CD film against E. coli and S. aureus reached 92 % and 95 %, respectively, which was attributed to the slow release of Cur when Cur@β-CD was dissolved in PVA material and the Schiff base reaction between Cur and amino groups on CS. These findings indicate that the PCOMC-Cur@β-CD film developed in this work can provide certain insights into the field of food packaging.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Zhihong Yin,Liandong Zhu,Xiaowen Shi,Francesco G. Gentili,Yohanes Kristianto Nugroho
Flocculation is one of the promising methods for harvesting microalgae. In this study, a series of grafted cationic chitosan-cyclodextrin composite flocculants (CTS/β-CDs) with different structural properties were prepared from chitosan and cyclodextrin. The results showed that the harvesting efficiency of CTS/β-CD-1 for C. vulgaris reached 99.91%. The chain type of polymeric flocculants with moderate graft chain lengths (L = 10–20) and tighter branched chain density distribution (D = 1.0–1.8) was favorable for C. vulgaris harvesting. The addition of CTS/β-CDs caused the discrete C. vulgaris cells to be flocculated and aggregated, and the reorganization of particles resulted in larger particles after flocculation, improving the dewatering performance of microalgae flocs. CTS/β-CDs had no significant inhibitory effect on the extraction of lipids, proteins and carbohydrates, and did not affect the extraction and quality of fatty acid methyl esters, and the re-cultivation experiments of the harvested water showed that chitosan-based flocculant was able to effectively reduce the cost of microalgae harvesting. Electrostatic interactions and bridging were the main harvesting mechanisms between CTS/β-CD-1 and C vulgaris . E-DLVO modeling suggested that flocculation is mainly a result of electrostatic and acid-base interactions when the distance between particles is small. At higher pH, the harvesting efficiency of CTS/β-CD-1 with C. vulgaris decreased. The study contributes to a better understanding of the structure–activity relationship between the structural characteristics of flocculants and the harvesting performance.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Ling Li,Yanxin Huang,Qianqian Ding,Danli Wang,Tinglan Yuan,Gongshuai Song,Hyunbin Seong,Jinyan Gong
Milk protein and hyaluronic acid (HA) at different mass ratios were systemically characterized using the Maillard reaction. Combining FTIR and molecular docking results, both conjugates, α-casein-HA (α-CN-HA) and β-lactoglobulin-HA (β-Lg-HA), were prepared via hydrogen-bond interactions. Binding of HA promotes the structural folding of milk proteins and alters their secondary structures. The α-CN-HA and β-Lg-HA conjugates exhibited significantly improved solubility thermal and antioxidant activity. The functional properties of the conjugates were modulated by varying the ratio of milk proteins to HA. The α-CN:HA 1:2 ratio had the highest denaturation temperature (110.1 °C). For β-Lg-HA, the highest denaturation temperature (112.1 °C) was observed at a 1:4 ratio. Antioxidant capacity increased with decreasing HA content; the highest value was observed at a mass ratio of 3:1 for both conjugates. Our findings suggest that Maillard reaction improves the physiological and functional properties of milk proteins and HA conjugates.
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Desalination and Water Treatment
·
2024
·
IF 1
Dezhi Qin,Wenhui Hu,Li Zhang
The Fe 3 O 4 /NiO magnetic nanomaterials were prepared by microwave-assisted and hydrothermal synthesis in β-cyclodextrin (β-CD) solution, which can be employed as absorbents and photocatalysts for the degradation of Congo red (CR). The XRD, EDX and TEM characterizations were used to determine the structure, composition and morphology of the as-prepared samples. The interaction between Fe 3 O 4 /NiO nanoparticles and β-CD molecules was investigated by TGA and FT-IR spectra. The results indicate that β-CD molecules can encapsulate the as-prepared nanoparticles and control the nucleation and growth of Fe 3 O 4 /NiO nanocrystals to certain extent. The pyrolysis of β-CD molecules and calcination treatment produced porous structure that provides more adsorption sites for CR molecules. The effect of reaction factors including calcination temperature and pH values was studied to determine the optimum condition. In addition, the thermodynamic and kinetic parameters of CR absorbed on Fe 3 O 4 /NiO nanoparticles were measured to understand the adsorption mechanism. The high removal efficiency for CR in aqueous solution was achieved within 60 min of adsorption and 40 min of UV-light irradiation, which can be attributed to the synergistic effect of the adsorption–photodegradation process. The prepared Fe 3 O 4 /NiO nanocrystals exhibit high adsorption capacity, magnetic separability, good photocatalytic activity and renewability, making it a potential material for wastewater treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Xin Yuan,Jia-Hui Liao,Gui-Jia Du,Yi Hou,Song-Qing Hu
Kinsenoside is a rare and valuable glycoside with extensive bioactivities. However, the enzymatic synthesis of kinsenoside has been a challenging task due to the limited enzyme toolbox and unsatisfactory yield. Herein, the β-glucosidase from Dictyoglomus thermophilum (DtBGL) was heterologously expressed, purified and enzymatically characterized. The purified DtBGL was successfully immobilized on the metal-organic frameworks of UiO-66-NH 2 . The DtBGL@UiO-66-NH 2 was fully characterized using SEM, XRD, TGA and FTIR. The studies on enzymatic properties demonstrated that DtBGL@UiO-66-NH 2 exhibited increased catalytic activity and stability compared to the free DtBGL. Particularly, DtBGL@UiO-66-NH 2 could catalyze the synthesis of kinsenoside via the reverse hydrolysis reaction and the kinsenoside yield was 34.12 % under the optimized catalytic system, which was 1.9-fold higher compared with the free DtBGL. Moreover, DtBGL@UiO-66-NH 2 displayed good reusability with a kinsenoside yield of 27.02 % after reuse for 3 times. The present work not only identifies and characterizes a highly active β-glucosidase with reverse hydrolysis activity, but also proposes the immobilized enzyme as an effective catalyst for the industrial production of glycosides.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Jinyu Chen,Beibei Jia,Siyang Wang,Zhuoling Li,Zhirui Ji,Ximing Li,Zijian Wu
Generally, high levels of natural phenolic antioxidants, which are needed in meat products to exert equivalent antioxidant effects as synthetic antioxidants, can interact with myofibrillar proteins and cause aggregation of the proteins, giving rise to undesirable deterioration of gel quality. In this study, β-cyclodextrin-metal-organic frameworks (K-β-CD-MOFs) were synthesized based on β-CD and K + ions using methanol vapor diffusion method, and used to inhibit excessive covalent and non-covalent interactions between gallic acid (GA) and myofibrillar proteins under oxidative stress without degrading the phenolic antioxidant activity . Results of zeta potential , scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC) provided evidence for the formation of GA/K-β-CD-MOFs inclusion complexes. From sulfydryl content, surface hydrophobicity , tryptophan fluorescence, turbidity and particle size analyses, the presence of K-β-CD-MOFs dose-dependently prevented the sulfydryl loss, unfolding of the proteins and formation of insoluble aggregates caused by GA (180 μM/g protein). Dynamic rheology, cooking loss, gel strength, water distribution, microstructure and Raman spectrum were further tested to evaluate effects of K-β-CD-MOFs on the qualities of GA-treated myofibrillar protein gel. Results indicated that more proteins were involved in the formation of a more ordered and homogeneous gel structure with higher gel elasticity and strength, more immobilized water and decreased cooking loss. The overall gel properties of myofibrillar proteins were improved. K-β-CD-MOFs ameliorated the instability of protein structure, manifested as increase in β-sheet content and inter-chain hydrogen bonding and decrease in hydrophobic forces ( p < 0.05). This paper provides a novel method to increase the loading amount of phenolic antioxidants without jeopardizing the gel quality of meat products.
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Science China-Materials
·
2024
·Vol. 67
·期 5
·1533-1542
·
IF 6.8
Yang Dezhen,Sun Chuanqiang,Han Zhe,Yuan Baogang,Pan Dong,Chen Jinchuan,Xu Huajie,Liu Chuntai,Shen Changyu
Silk fibroin (SF), as a special natural polymer, is a good candidate for preparing flexible sensor due to its excellent biocompatibility, biodegradability and amphiphilic properties. However, the high and uncontrollable crystallization of SF makes it challenge to be utilized in excellent biomimetic SF hydrogel sensor. Neat SF hydrogel is brittle and not adhesive. Here, polyacrylamide (PAM) was introduced to the SF hydrogel to address this issue. Due to their strong intermolecular interaction, the network morphology of SF/PAM hydrogel was altered to non-Euclidean pore from Euclidean one. Meanwhile, its β-sheet crystallization was suppressed to nanoscale readily. These evolutions endow the SF/PAM hydrogel with not only enhanced mechanical properties but also excellent adhesion performance. Compared with that of neat PAM hydrogel, tensile strength, tensile failure strain, compressive strength (under 80% strain) and adhesion performance (on pig skin) of the SF8/PAM hydrogel are increased by 133.1%, 120.9%, 610.8% and 104.8%, respectively. Additionally, the amphiphilicity of SF could make the carbon nanotubes (CNTs) disperse well in hydrogel. CNT0.3/SF8/PAM hydrogel not only inherits but also improves the above properties. It exhibits excellent adhesive sensing performance with maximal gauge factor of 10.13, working strain range of 1000% and stability as long as over 500 cycles. The detection of six human activities was also demonstrated. This work affords a general strategy to achieve high-performance SF-based hydrogel and indicates that the SF/PAM hydrogel possesses great promise for applications in flexible wearable strain sensors.
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BIOMEDICINE & PHARMACOTHERAPY
·
2024
·
IF 6.9
Na Wang,Anqi Yang,Xiong Tian,Jiaqi Liao,Zhenyu Yang,Yixiao Pan,Yiqing Guo,Sailing He
Background Mitochondrial redox imbalance underlies the pathophysiology of type2 diabetes mellitus (T2DM), and is closely related to tissue damage and dysfunction. Studies have shown the beneficial effects of dietary strategies that elevate β-hydroxybutyrate (BHB) levels in alleviating T2DM. Nevertheless, the role of BHB has not been clearly elucidated. Methods We performed a spectral study to visualize the preventive effects of BHB on blood and multiorgan mitochondrial redox imbalance in T2DM mice via using label-free resonance Raman spectroscopy (RRS), and further explored the impact of BHB therapy on the pathology of T2DM mice by histological and biochemical analyses. Findings Our data revealed that RRS-based mitochondrial redox states assay enabled clear and reliable identification of the improvement of mitochondrial redox imbalance by BHB, evidenced by the reduction of Raman peak intensity at 750 cm −1 , 1128 cm −1 and 1585 cm −1 in blood, tissue as well as purified mitochondria of db/db mice and the increase of tissue mitochondrial succinic dehydrogenase (SDH) staining after BHB treatment. Exogenous supplementation of BHB was also found to attenuate T2DM pathology related to mitochondrial redox states, involving organ injury, blood glucose control, insulin resistance and systemic inflammation. Interpretation Our findings provide strong evidence for BHB as a potential therapeutic strategy targeting mitochondria for T2DM.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Meng Wu,Laijun Xu,Fei Xing,Rongjun Xiao,Wangping Wu
Despite the clinical success of tricalcium silicate (TCS)-based materials in endodontics, the inferior handling characteristic, poor anti-washout property and slow setting kinetics hindered their wider applications. To solve these problems, an injectable fast-setting TCS/β-tricalcium phosphate/monocalcium phosphate anhydrous (β-TCP/MCPA) cement was developed for the first time by incorporation of hydroxypropyl methylcellulose (HPMC) and β-TCP/MCPA. The physical-chemical characterization (setting time, anti-washout property, injectability, compressive strength, apatite mineralization and sealing property) of TCS/(β-TCP/MCPA) were conducted. Its hydration mechanism was also investigated. Furthermore, the cytocompatibility and osteogenic/odontogenic differentiation of stem cells isolated from human exfoliated deciduous teeth (SHED) treated with TCS/β-TCP/MCPA were studied. The results showed that HPMC could provide TCS with good anti-washout ability and injectability but slow hydration process. However, β-TCP/MCPA effectively enhanced anti-washout characteristics and reduced setting time due to faster hydration kinetics. TCS/(β-TCP/MCPA) obtained around 90 % of injection rate and high compressive strength whereas excessive additions of β-TCP/MCPA compromised its injectability and compressive strength. TCS/(β-TCP/MCPA) can induce apatite deposition and form a tight marginal sealing at the dentin-cement interface. Additionally, TCS/(β-TCP/MCPA) showed good biocompatibility and promoted osteo/odontogenic differentiation of SHED. In general, our results indicated that TCS/(β-TCP/MCPA) may be particularly promising as an injectable bioactive cements for endodontic treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shuo Sun,Xingyu Fu,Xu Yang,Shuhui Zhao,Huitian Zhi,Ruhui Shang,Xujie Wang,Yingchao Yang,Zhengquan Yan,Lei Hu
To high-performance recognize or degrade o/p -aminophenol and methyl red, nano Zr/Cu oxide decorated poly-β-cyclodextrin (CuO/ZrO 2 @p-β-CD) was developed under microwave-assisted conditions. Fibrous CuO/ZrO 2 @p-β-CD expressed synergistic peroxidase-like activity to degrade >90.4 % methyl red in the air. When applied to accelerate the oxidization of 2,2′-azinobis (3-ethyl benzothiazoline-6-sulfonic acid) ammonium salt (ABTS), K m / V max could be 0.053 mM/2.92 × 10 −8 M/s. Trace aminophenol isomers ( o -AP and m -AP) could exclusively alter CuO/ZrO 2 @p-β-CD-ABTS from green to colorless or to purple-red with remarkable time-resolved effect. Under the optimized conditions, CuO/ZrO 2 @p-β-CD was successfully applied for intelligent monitoring or degradation o -AP, m -AP or methyl red in practice with the help of a smartphone. The recoveries for UV–vis spectral/RGB detection of o -AP and m -AP could reach 100.0 % ~ 102.9 %/98.0 % ~ 102.5 % and 97.8 % ~ 101.1 %/96.8 % ~ 103.33 %. The catalytic detection or quantitative degradation mechanisms were investigated in detail. This work will provide a potential for quantitative monitoring or degradation of organic pollutions.
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危险品化学品经营许可证（带存储）