BMC CANCER
·
2022
·Vol. 22
·期 1
·1-9
·
IF 4.638
Chen Zibo,Yuan Tao,Yan Fangjie,Ye Song,Xie Qin,Zhang Bo,Lin Nengmin,He Qiaojun,Yang Bo,Zhu Hong
Background Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. Sorafenib is the first-line treatment for advanced HCC, but the anti-cancer effects remain to be improved as indicated by its low response rates and failure to prolong the progression-free survival (PFS). Thus, it is urgent to explore approaches to improve the clinical outcome.Materials and methodsThe effect of Sorafenib in HCC was analyzed by SRB (sulforhodamine B) assay in normoxia and hypoxia, respectively. The different dose combination effect of CT707 and sorafenib was analyzed by SRB assay in hypoxia. Flow cytometry assay was used to detect the cell apoptosis rate with CT707 and sorafenib treatment in hypoxia. Western blotting was used to detect the expression levels of apoptosis -related proteins and the mechanism of CT707 overcome the resistance of sorafenib in hypoxia.Results Our study showed that the characteristic intratumor hypoxia of advanced HCC is one of the major factors which mediated the drug resistance towards sorafenib in HCC. And CT-707, a novel multi-kinase inhibitor, could sensitize the hypoxic HCC cells towards sorafenib. Further studies showed that CT-707 abolished the nuclear translocation of Yes Associate-Protein (YAP), which has been demonstrated as one of mechanism of hypoxia-mediated sorafenib-resistance in HCC.Conclusion sOverall, this study not only favors the development of this novel multi-kinase inhibitor CT-707 as a therapeutic agent against HCC, but also provides a potential strategy to overcome the hypoxia-mediated resistance to sorafenib in HCC patients.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 15
·12002-12015
·
IF 2.779
Wang Wenwei,Hu Yongcai,Li Liangjie,Zeng Jinling,Yao Yingbang
In this study, the contents of ethyl cellulose (EC) as thickener in organic vehicle, which also acts as a binder, was examined using various control experiments; dibutyl phthalate (DBP) and dioctyl phthalate (DOP) were used as plasticizers, and acrylic resin (AR) was used as the thixotropic agent. In addition to thixotropic index, Bigham and Casson yield stress models were used for simulation, and the organic vehicle was dynamically simulated via the three interval thixotropy test; the viscosity recovery of the organic vehicle was investigated on the removal of shear stress. When the contents of the plasticizers, DBP and DOP, were 4 wt% and 6 wt%, respectively, the content of the thickener, EC, was 12 wt% and that of the thixotropic agent, AR, was 2 wt%, the comprehensive performance of the organic vehicle was greatly improved, especially in terms of thixotropy and recovery behavior. Our results demonstrate that the current organic vehicle is promising for the application of silver pastes used as inner electrodes in low-temperature co-fired ceramic (LTCC).
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 16
·7791-7803
·
IF 4.682
Ma Yanli,Wu Hailiang,Shen Yanqin
We herein report novel POSS-containing linear and star multi-arm organic–inorganic hybrid block copolymers (BCPs) L/S -(PGMA- b -PMAPOSS) 1,2,4,6 synthesized by the core-first ATRP method. Following, crosslinking of the BCPs conducted by the reaction of the glycidyl groups in the PGMA block in the presence of trimethylamine to form the 3-dimensional network and the crosslinking cured BCPs were used for the subsequent properties characterization. Based on the chemical structures being confirmed, the self-assembly behaviors in the aqueous solution and film surface property were investigated in detail. The results showed that linear L -(PGMA- b -PMAPOSS) 2 formed typical core–shell spherical micelles where the hydrophobic PMAPOSS blocks as the core and PGMA blocks as the shell. With the increasing number of arms of the hybrid BCPs, the aggregates were tuned from spherical micelles to dendritic cylinders. Besides, with the increases in the solubility of the solvent, the aggregates tended to form the spherical morphology. The hydrophobic property of the hybrid BCPs film improved gradually from linear monobrachial BCPs to star six-arm BCPs. The thermostability of the hybrid BCPs showed similar results owing to the similar MAPOSS proportion. For the adhesive performance to the glass substrate, S -(PGMA- b -PMAPOSS) 6 possessed the best adhesive strength due to the highest density of branches. Graphical abstract
The novel dual-functional linear and star POSS-containing organic-inorganic hybrid block copolymers (BCPs) L/S -(PGMA- b -PMAPOSS) 1,2,4,6 were successfully synthesized by the core-first ATRP method, then crosslinking the BCPs in the presence of trimethylamine to form the 3-dimensional network. And the self-assembly behavior in aqueous solution was studied in detail, as shown in the graphic image. In addition, the surface property, thermostability and adhesive performance were analyzed.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 16
·7909-7922
·
IF 4.682
Chai Chaofan,Yang Xuefang,Yang Xiaohua,Dong Chuan,Bian Wei,Choi Martin M. F.
Abstract The utilization of nanomaterial-based probes in detecting glutathione (GSH) and cell imaging has aroused extensive attention owing to the excellent properties of nanoprobes. Herein, we have synthesized manganese dioxide-S, O co-doped graphitic carbon nitride quantum dots (MnO 2 -S, O-CNQDs) nanocomposite by in situ synthesis of MnO 2 nanosheets in S,O-CNQDs dispersion solution. It was found that GSH could specifically bind to MnO 2 -S, O-CNQDs so that the fluorescence of S, O-CNQDs could be recovered. As such, a “turn-on” MnO 2 -S, O-CNQDs nanoprobe can be fabricated and applied to rapidly determine trace amounts of GSH. Under the optimal conditions, MnO 2 -S, O-CNQDs shows sensitive response to GSH in the range 10–270 μM with a detection limit of 0.307 μM ( S / N = 3). The developed MnO 2 -S, O-CNQDs probe has demonstrated great potential to detection of GSH in biological samples and glutathione injections. What is more, MTT assay indicates that MnO 2 -S, O-CNQDs has low biotoxicity. The non-fluorescence MnO 2 -S, O-CNQDs reacts with GSH to recover the fluorescence of S, O-CNQDs in HepG2 cells. Thus, the “turn-on” fluorescence change of MnO 2 -S, O-CNQDs offers a potentially useful tool to monitor GSH of cancer cells. Graphical Abstract
✓ 已验证
产品关联
DOI
高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2022
·Vol. 32
·期 8
·2986-2993
·
IF 3.518
Hao Bei-bei,Deng Xiu-zhen,Yang Jia-ke,Jia Ying-dong,Shang Xiao-jun,Shi Yong-li,Yan Xi-qing
A novel folate-decorated and magnetic mediated drug delivery system was fabricated by combining folate and magnetic nanoparticles to the surface of silica mesoporous nanoparticles (FMMSNs) via chemical reaction. The 10-hydroxycamptothecin (10-HPCT), an analogue of camptothecin, was sealed in the mesoporous to evaluate the system’s pharmaceutics performance. The diameter of the FMMSMs was about 100 nm and the in vitro release result showed that the applied magnetic field could influence the release process. The result of the inhibition rate of the 10-HPCT loaded FMMSNs to HepG-2 cells revealed that they could be influenced by the applied magnetic field or the folic acid bonded on the surface of the mesoporous nanoparticles.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-18
·
IF 9.429
Xia Ya-Nan,Zu He,Guo Haoxiang,Jiang Tianyan,Yang Siqi,Yu Huan,Zhang Shaodian,Ding Heng,Li Xiaoyu,Wang Yangyun,Wang Yong,Zhang Leshuai W.
Albumin-biomineralized copper sulfide nanoparticles (Cu 2−x S NPs) have attracted much attention as an emerging phototheranostic agent due to their advantages of facile preparation method and high biocompatibility. However, comprehensive preclinical safety evaluation is the only way to meet its further clinical translation. We herein evaluate detailedly the safety and hepatotoxicity of bovine serum albumin-biomineralized Cu 2−x S (BSA@Cu 2−x S) NPs with two different sizes in rats. Large-sized (LNPs, 17.8 nm) and small-sized (SNPs, 2.8 nm) BSA@Cu 2−x S NPs with great near-infrared absorption and photothermal conversion efficiency are firstly obtained. Seven days after a single-dose intravenous administration, SNPs distributed throughout the body are cleared primarily through the feces, while a large amount of LNPs remained in the liver. A 14-day subacute toxicity study with a 28-day recovery period are conducted, showing long-term hepatotoxicity without recovery for LNPs but reversible toxicity for SNPs. Cellular uptake studies indicate that LNPs prefer to reside in Kupffer cells, leading to prolonged and delayed hepatotoxicity even after the cessation of NPs administration, while SNPs have much less Kupffer cell uptake. RNA-sequencing analysis for gene expression indicates that the inflammatory pathway, lipid metabolism pathway, drug metabolism-cytochrome P450 pathway, cholesterol/bile acid metabolism pathway, and copper ion transport/metabolism pathway are compromised in the liver by two sizes of BSA@Cu 2−x S NPs, while only SNPs show a complete recovery of altered gene expression after NPs discontinuation. This study demonstrates that the translational feasibility of small-sized BSA@Cu 2−x S NPs as excellent nanoagents with manageable hepatotoxicity. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Friction
·
2022
·Vol. 10
·期 11
·1824-1837
·
IF 4.924
Liu Shutian,Dong Conglin,Yuan Chengqing,Bai Xiuqin
Natural materials tend to exhibit excellent performance in the engineering field because of their structure and special functions. A natural red willow, called natural porous wood material (NPWM), was found, and wear tests were conducted to determine its potential as an oil-impregnated material by utilizing its special porous structure. Fluorination treatment was adopted to improve the NPWM properties for absorbing and storing lubricating oil. The different contributions of soaking and fluorination-soaking treatments on the tribological properties of NPWMs and their respective mechanism of effect were revealed. The results showed that the fluorination-soaking treatment helped absorb and store sufficient lubricating oil in the NPWM porous structure; therefore, more lubricating oil would be squeezed out and function as a tribol-film between contacting surfaces during the friction process, thus ultimately contributing to stable and smooth wear responses even under prolong friction. However, the formation of an oil-in-water emulsion, caused by the buoyancy effect, destroyed the oil films on the worn NPWM surface in a water environment, resulting in higher coefficients of friction (COFs) under water conditions than under dry friction, even after the fluorination-soaking treatment. The knowledge gained herein could not only verify the potential of NPWM as an excellent oil-impregnated material in the engineering field but also provide a new methodology for the design of artificial porous materials with stable and smooth friction processes.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 16
·12808-12818
·
IF 2.779
Li Qihui,Du Haiying,Li Junrui,Deng Jie,Wang Ruiyuan,Chen Yonglin
Tartrazine is used as a synthetic colorant to improve the color of food. Excessive intake of tartrazine will seriously harm human health. In this paper, a green and facile one-pot hydrothermal approach is proposed for the preparation of sulfur-doped carbon quantum dots ( S- CQDs) with Alternanthera philoxeroides (AP) powder and thiourea. The S- CQDs were developed as a highly sensitive “turn-off” fluorescent sensor for the detection of tartrazine. The S- CQDs displayed superior fluorescence characteristics of CQDs with a quantum yield (QY) up to 20.19%. S- CQDs exhibited the maximum fluorescence emission intensity at λ em = 462 nm under excitation at λ ex = 390 nm. The standard curve of S- CQDs had good linearity in the range of 7.8 ~ 125 μmol/L tartrazine, and the linear correlation coefficient ( R 2 ) was 0.9983. The S- CQDs were demonstrated to selectively react with tartrazine, leading to fluorescence quenching effect, which was successfully used for the detection of tartrazine with a limit of detection at 0.45 μmol/L, which was lower than the national standard detection limit of 187.14 μmol/L. The feasibility of practical application was assessed by the detection of tartrazine in real samples.
✓ 已验证
产品关联
DOI
高影响因子
CELLULOSE
·
2022
·Vol. 29
·期 10
·5511-5527
·
IF 6.123
Zhang Shuaifeng,Gu Jun,Fan Baochao,Li Li,Li Bin
We report a new method for treating high-molecular-weight cellulose with 60 Co gamma rays to simultaneously graft functional groups onto the natural polymer and promote its solubility. After exposing cellulose to a 40-kilogray dose of gamma rays in the presence of 2-methylacrylamide, numerous amide groups were grafted onto the cellulose chain and its solubility increased. Amide-functionalized cryogels were prepared via the sol–gel method using the irradiated product as a raw material. Compared with 40-kGy-irradiated high-molecular-weight-cellulose cryogel, the amide-functionalized cryogels had relatively high zero-point charge pH values and excellent adsorption capacities with regard to anionic dyes over the pH range 2–10. They were also stable in terms of reusability. Therefore, the 2-methylacrylamide/high-molecular-weight cellulose cryogel has great potential for use in the treatment of colored surface wastewater. The 60 Co gamma ray irradiation technique described herein is a stable and highly efficient method for the preparation of functionalized cellulose products. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 5
·1-10
·
IF 6.408
Ding Jinglei,Zhang Wenqi,Xue Fengying,Sun Yu,Yan Quizhu,Chen Yanwei,Shan Guiye
Gold nanoclusters (AuNCs) are widely used in the fluorescence detection of biomolecules in human serum due to their good fluorescence properties, low toxicity, and better biocompatibility. However, the weak fluorescence intensity of AuNCs limits the fluorescence detection of molecules within a wide concentration range. It is reported that coating AuNCs in ZIF-8 with adjustable pore size can effectively improve the fluorescence intensity of AuNCs and broaden the detection range. And the AuNCs wrapped in the gaps of ZIF-8 can prevent the fluorescence quenching caused by the aggregation of AuNCs. However, ZIF-8 has high crystallinity, poor dispersion, and easy deposition, which reduces the fluorescence stability of the detection system and affects the detection. Based on the above research, the highly hydrophilic polymer PEI was modified to the surface of ZIF-8, and a kind of nanocomposite material AuNCs/ChOx@ZIF-8/PEI was obtained by co-encapsulating AuNCs prepared with glutathione as a ligand and cholesterol oxidase (ChOx) into ZIF-8 modified with PEI. The composite material emits strong red light at 650 nm under the excitation of 395-nm light, and the system can sensitively detect cholesterol (Chol) in human serum. Compared with other materials, the PEI-modified composite has better solubility and stability, so the detection effect of Chol is better. Encapsulation of ChOx in the ZIF-8 shell can protect the enzyme and increase the local concentration of ChOx, thereby speeding up the reaction rate. Compared with free AuNCs/ChOx, the quenching rate of AuNCs/ChOx@ZIF-8/PEI system is doubled. Secondly, the addition of Fe 2+ to the detection process results in higher quenching rate and detection sensitivity. The system can detect Chol in the concentration range 0.1–2.4 μM, with a detection limit of 0.073 μM. The determination is a fast and sensitive strategy. In addition, the practicability of this assay in the detection of Chol in human serum has been verified. Due to its selectivity and sensitivity, it has potential application value in clinical diagnosis. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Probiotics and Antimicrobial Proteins
·
2022
·Vol. 14
·期 4
·741-750
·
IF 5.265
Gao Xiaoxi,Zhang Yuhong,Mu Guangqing,Xu Yunpeng,Wang Xinmiao,Tuo Yanfeng,Qian Fang
The aim of the present study was to investigate the in vitro antioxidant potential of Bacillus coagulans T242. B. coagulans T242 showed better antioxidant activities, including the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging ability, lipid peroxidation inhibiting ability and reducing ability, than those exerted by Lactobacillus rhamnosus GG (LGG). B. coagulans T242 positively regulated the expression of the nuclear factor erythroid 2-relatedfactor 2/Kelch-like ECH-associated protein-1 (Nrf2/Keap1) pathway-related proteins (Nrf2, Keap1, heine oxygenase-1 (HO-1)); increased antioxidant enzymes (glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD)); reduced the content of malondialdehyde (MDA) level; decreased the expression of inflammatory-related cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α); and thus increased the survival rate in 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH)-damaged HT-29 cells. This study proved that B. coagulans T242 exerted antioxidative effects by quenching oxygen free radicals and activating the Nrf2 signaling pathway in HT-29 cells.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
·
2022
·Vol. 5
·期 3
·1852-1864
·
IF 11.806
Zhang Feng,Lian Mengying,Alhadhrami A.,Huang Mina,Li Bin,Mersal Gaber A. M.,Ibrahim Mohamed M.,Xu Miaojun
Enzyme immobilization technique has a broad application prospect in the area of biotechnology, biochemistry, and environmental remediation. In this study, the composite hydrogel consisting of dopamine functionalized cellulose nanofiber and alginate was fabricated for immobilization of laccase. Infrared spectra, scanning electron micrographs, and energy dispersive X-ray analysis demonstrated that the laccase molecules were covalently immobilized onto the surface of composite hydrogel. The immobilized laccase exhibited the highest enzymatic activity about 462 U·g −1 . Compared with free laccase, the pH, temperature, and storage stabilities of immobilized laccase were improved dramatically. Moreover, the immobilized enzyme can be easily separated from reaction system and reused. It retained 79.6% of its initial activity after 14 cycles of operation. Immobilized laccase was utilized for efficient degradation of bisphenol A (BPA) from polluted water, and the affecting factors on BPA removal efficiency were analyzed. Greater than 82% of BPA was removed under optimum conditions. A small amount of ABTS was added into BPA degradation; the removal efficiency had reached up to 98.7%. Results indicated that the immobilized laccase has great potential for industrial applications. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）