JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-17
·
IF 10.435
Lan Biyun,Zhang Liming,Yang Liqun,Wu Junfeng,Li Na,Pan Chenglin,Wang Xiaoyi,Zeng Lexiang,Yan Li,Yang Chuan,Ren Meng
AbstractExcessive expression of matrix metalloproteinase 9 (MMP-9) impedes healing of diabetic chronic wounds, thus wound dressing that could effectively inhibit the expression of MMP-9 offers significant clinical translation for diabetic wound healing. Herein, a hybrid hydrogel dressing was developed for localized and sustained delivery of MMP-9 siRNA (siMMP-9). siMMP-9 was complexed with Gly-TETA (GT), the GT/siMMP9 complex was then loaded into a thermosensitive hydrogel based on Pluronic F-127 (PF) and methylcellulose (MC). In vitro, this hybrid hydrogel dressing exhibited negligible cytotoxicity, prolonged the release of GT/siMMP-9 for up to 7 days, and significantly reduced MMP-9 expression. In vivo assessment in diabetic rats demonstrated that hydrogel provided localized and sustained delivery via the thermosensitive controlled release of entrapped GT/siMMP-9 into wound tissues for 7 days, resulting in dramatic MMP-9 silencing which significantly improved diabetic wound closure. This hybrid hydrogel dressing exhibited excellent biocompatibility, with no observed systemic toxicity in rats. Taken together, the hybrid hydrogel dressing may constitute an effective and biocompatible means of enhancing diabetic wound healing through effective silencing of the MMP-9 gene, and this hydrogel delivery system also offers a platform for in vivo delivery of siRNA for the treatment of other diseases.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·784
·
IF 12.6
Lv Jiali,Cheng Chen,Liu Xinran,Song Jinhua,Li Daxiang,Wang Yijun,Du Yiqun
The physicochemical properties of oral drug delivery carriers significantly influence their interactions with the intestinal barrier, which, in turn, affect their in vivo fate and therapeutic efficacy. As a delivery system with a hierarchical structure and high stability, Pickering emulsions have demonstrated significant advantages in enhancing the bioavailability of encapsulated bioactive compounds. However, the specific role of their inherent flexibility in modulating interactions with the intestinal barrier and influencing delivery efficiency remains poorly understood. In this study, a flexible Pickering emulsion (FPPE) stabilized by chitosan nanoparticles was developed for encapsulating epigallocatechin gallate, aiming to investigate the role and underlying mechanism of its flexible structure in mucus penetration, cellular uptake, and biodistribution in the intestinal tract. Upon reaching the intestinal tissue, FPPE exhibited deformable adaptation, facilitating efficient mucus permeation, prolonged gastrointestinal retention, and enhanced drug absorption. Moreover, FPPE enhanced macrophage uptake and mitochondrial membrane potential, reduced reactive oxygen species scavenging, and triggered macrophage reprogramming. Compared to rigid chitosan microspheres, FPPE more effectively alleviated intestinal inflammation and promoted intestinal barrier repair in a dextran sulfate sodium-induced acute colitis mouse model. This study reveals how Pickering emulsion flexibility facilitates intestinal mucus delivery, guiding their future design and application. Graphical
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Nature Communications
·
2025
·
IF 15.7
Chen Long,Zhang Zonghao,Li Penghao,Luo Hanzhuo,Li Chenchen,Pan Sihui,Huang Wei-Hsiang,Pao Chih-Wen,Yang Hao,Hu Zhiwei,Shao Qi
Electrocatalytic acetone hydrogenation into high-value alcohols offers a sustainable alternative to thermal methods. However, developing non-noble metal catalysts with superior performance for electrocatalytic acetone hydrogenation remains challenging. Two-dimensional non-noble metal structure with metastable-phase active sites is promising due to its high-energy structure and unique electronic environment. However, its precise synthesis remains a great challenge. In this work, we report a two-dimensional metastable hexagonal close-packed phase nickel nanosheets (hcp Ni NSs) with a space group of P6 3 /mmc. In the electrocatalytic acetone hydrogenation, it achieves 95.0 % acetone conversion with 100 % isopropanol selectivity. Notably, in the scale-up experiment, the hcp Ni NSs also exhibit 11.51 g g cat -1 h -1 isopropanol production rate at a current density of −250 mA cm -2 . Furthermore, this catalyst is widely applicable to various carbonyl compounds. Mechanism study shows that hcp Ni NSs enable strong acetone adsorption and rapid isopropanol desorption.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-9
·
IF 5.705
Gou Dan,Xie Guoming,Li Yuxia,Zhang Xin,Chen Hui
The authors describe an electrochemical immunoassay for ultrasensitive determination of the Mycobacterium tuberculosis (MTb) secretory protein MPT64 which is an antigen for early diagnosis of infection with MTb . Protein G was used to immobilize antibodies against MPT64 on a gold electrode. Graphene oxide with its large surface area was used as a carrier to anchor magnetite (Fe 3 O 4 ) and platinum (Pt) nanoparticles. The nanocomposite of type GO@Fe 3 O 4 @Pt was used as a signal reporter with excellent catalytic activity and recyclability. The nanocomposite exhibits peroxidase-like activity for hydrogen peroxide, best at −0.25 V (vs. Ag/AgCl). It works over a wide range of pH values (2–10) and temperatures (25–65 °C). Further signal amplification strategy was accomplished by rolling circle amplification. This voltammetric assay has a linear response to the logarithm of MPT64 concentration in the range from 5.0 fg·mL −1 to 1.0 ng·mL −1 and a detection limit of 0.34 fg·mL −1 (at a signal to noise ratio of 3, for n = 10). The assay can be completed within 4 h. It was successfully applied to the determination of MPT64 in spiked serum samples. Conceivably, the assay has a large potential in providing laboratory evidence for rapid diagnosis of MTb infection.
An electrochemical biosensor was developed for rapid detection of Mycobacterium tuberculosis secretory protein MPT64. The detection signal was synergistically amplified by GO@Fe 3 O 4 @Pt and rolling circle amplification. Phi29: phi29 DNA polymerase; BSA: bovine serum albumin; GO: graphene oxide.
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JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
·
2016
·Vol. 21
·期 8
·945-956
·
IF 2.495
Sun Dongdong,Mou Zhipeng,Li Nuan,Zhang Weiwei,Wang Yazhe,Yang Endong,Wang Weiyun
Two new ruthenium (II) polypyridyl complexes [Ru(MeIm) 4 (pip)] 2+ ( 1 ) and [Ru(MeIm) 4 (4-npip)] 2+ ( 2 ) were synthesized under the guidance of computational studies (DFT). Their binding property to human telomeric G-quadruplex studied by UV–Vis absorption spectroscopy, the fluorescent resonance energy transfer (FRET) melting assay and circular dichroism (CD) spectroscopy for validating the theoretical prediction. Both of them were evaluated for their potential anti-proliferative activity against four human tumor cell lines. Complex 2 shows growth inhibition against all the cell lines tested, especially the human lung tumor cell (A549). The RTCA analysis not only validated the inhibition activity but also showed the ability of reducing A549 cells’ migration. DNA-flow cytometric analysis, mitochondrial membrane potential (ΔΨm) and the scavenger measurements of reactive oxygen species (ROS) analysis carried out to investigate the mechanism of cell growth inhibition and apoptosis-inducing effect of complex 2 . The results demonstrated that complex 2 induces tumor cells apoptosis by acting on both mitochondrial homeostasis destruction and death receptor signaling pathways. And those suggested that complex 2 could be a candidate for further evaluation as a chemotherapeutic agent against human tumor.
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CELLULOSE
·
2019
·Vol. 26
·期 13
·8087-8099
·
IF 3.917
Zhang Su-Feng,Liu Li-Na,Tang Rui-Hua,Liu Zhi,He Xiao-Cong,Qu Zhi-Guo,Li Fei
Abstract Lateral flow assays (LFAs) have been extensively used as point-of-care testing platforms because they are inexpensive, portable, simple, and rapid, which particularly improve their availability in resource-poor settings. However, the poor sensitivity of LFAs restricts their further applications. Herein, we proposed a novel and simple method to enhance the detection sensitivity of LFAs by embedding cotton thread-based barriers into paper and further integrating them into strips to decrease the flow rate of sample and extend the reaction time. The number and hydrophilicity of the embedded cotton threads were sequentially optimized. The flow rate of liquid in cotton thread-embedded LFAs was mathematically simulated using a circuit-like model and the simulation results are consistent with the experimental results. With using human immunodeficiency virus nucleic acid as a model target, the cotton thread-embedded LFAs presented a fourfold enhancement in detection sensitivity compared to that of the unmodified LFAs. The strategy of embedding cotton threads into paper possesses great potential for fabricating other paper-based microfluidic devices in the future. Graphic abstract
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 5
·1-6
·
IF 5.705
Liu Renjie,Wu Hua,Lv Lei,Kang Xiaojiao,Cui Chengbi,Feng Jin,Guo Zhijun
This study describes an aptamer based assay for the mycotoxin ochratoxin A (OTA). The method is based on the use of an OTA-specific aptamer, exonuclease (Exo) III, SYBR Gold as a fluorescent probe, and a complementary strand that specifically combines with the aptamer. In the presence of OTA, the aptamer and OTA hybridize, thereby resulting in the formation of ssDNA, which is not digested by Exo III. Intense fluorescence is observed after addition of SYBR Gold (best measured at excitation/emission wavelengths of 495/540 nm). Fluorescence increases linearly with the log of the OTA concentration in the range from 8 to 1000 ng · mL −1 . The detection limit is 4.7 ng·mL −1 . The assay was applied to the determination of OTA in diluted [2%( v /v)] red wine, and recoveries and RSDs ranged between 93.5% and 113.8%, and between 3.2% and 5.7%, respectively.
In the presence of ochratoxin A (OTA), specific combinations of aptamer and OTA may occur and result in DNA double strands being untied, which avoids being digested by Exo III. Intense fluorescence is observed after SYBR Gold addition.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2020
·Vol. 36
·期 2
·301-306
·
IF 1.063
Liu Shuo,Yang Mingjie,Guo Weiwei
Metal-organic frameworks(MOFs)-based nanozyme plays an important role in biosensing, therapy and catalysis. In this study, the effects of single-stranded DNA(ssDNA) with programmable sequences and its complementary DNA(TDNA) on the intrinsic peroxidase-like activity of hemin loaded MOFs(UiO-66- 2 ), denoted as hemin@UiO-66-NH 2 , were investigated. The hemin@UiO-66- 2 exhibited improved catalytic activity compared with free hemin. However, the catalytic activity is inhibited in the presence of ssDNA, as ssDNA can be adsorbed by MOFs and therefore protected the active sites from contact with substrates. Upon the addition of the T DNA , double-stranded DNA(dsDNA) was formed and detached from the MOFs, resulting in the recovery of catalytic activity. Sequentially adding ssDNA or its complementary DNA strands can achieve the reversible regulation of the catalytic activity of MOFs nanozymes. Moreover, the DNA hybridization-based regulation was further applied to a cascaded catalytic system composed of the nanozyme, hemin@UiO-66-NH 2 , and glucose oxidase. These nanozyme based programmable and reversibly regulated catalytic systems may have potential applications in future smart biosensing and catalysis systems.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·6783-6783
·
IF 4.259
Fang Liu,Na Zhang,Zhaojie Li,Xiong Wang,Hongjie Shi,Changhu Xue,Robert W. Li,Qingjuan Tang
Chondroitin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity. We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice. Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress. CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress. Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration. CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively. CS disaccharides reduced the prevalence of inflammatory Proteobacteria. Together, our findings demonstrated that CS may ameliorate stress-induced intestinal inflammation. Furthermore, CS significantly increased intestinal Bacteroides acidifaciens population, indirectly exerting its immunomodulatory effect on the intestine. CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism. Our results will facilitate the development of CS as a bioactive nutraceutical.
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BIOTECHNOLOGY LETTERS
·
2016
·Vol. 38
·期 6
·1009-1013
·
IF 1.639
Kang Le,Bai Yajun,Cai Yujie,Zheng Xiaohui
Objectives To characterize a novel feruloyl esterase from Escherichia coli BL21 DE3. Results The gene encoding BioH was cloned and overexpressed in E. coli . The protein was purified and its catalytic activity was assessed. BioH exhibited feruloyl esterase activity toward a broad range of substrates, and the corresponding kinetic constants for the methyl ferulate, ethyl ferulate, and methyl p -coumarate substrates were: K
m
values of 0.48, 6.3, and 1.9 mM, respectively, and k
cat
/ K
m
values of 9.3, 3.8, and 3.8 mM −1 s −1 , respectively. Conclusions Feruloyl esterase from E. coli was expressed for the first time. BioH was confirmed to be a feruloyl esterase.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 22
·27849-27858
·
IF 3.056
Ou Qi,Xu Shuxia,Long Yuanli,Zhang Xinfeng
The development of efficient solar driven catalyst for the degradation of antibiotics has become increasingly important in environmental protection. However, the reported efficient photocatalysts for antibiotic degradation are limited. In this work, porous Fe 3+ -doped graphitic carbon nitride (g-C 3 N 4 ) with outstanding photocatalytic ability is synthesized and then used as the photocatalyst for the efficient degradation of sulfadiazine (SDZ) under visible light. A series of characterization results indicate that Fe 3+ is successfully doped into the interlayer of g-C 3 N 4 and is stabilized in g-C 3 N 4 by Fe-N coordination bond. The SEM, DRS and ESI and transient photocurrent results demonstrated that Fe 3+ -doped g-C 3 N 4 has a porous structure, a low band gap, improved separation efficiency of photogenerated electron and holes as well as a wider light absorption range. Such improved physical and chemical properties greatly enhanced the photocatalytic ability. Using Fe 3+ -doped g-C 3 N 4 for photocatalytic degradation of SDZ under white light, almost complete degradation of SDZ was achieved with a degradation efficiency as high as 99.8% (whereas only 52.1% for bulk g-C 3 N 4 ) within 90 min. The degradation was mainly ascribe to 1 O 2 during the irradiation, and also a small amount of •O 2 − , OHand h + are involved in the degradation process. The Fe 3+ -doped g-C 3 N 4 was applicable for the degradation of a wide range of antibiotic pollutants.
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AAPS PHARMSCITECH
·
2018
·Vol. 19
·期 5
·2048-2057
·
IF 2.666
Zhai Qingqing,Li Hailong,Song Yanlin,Wu Ruijiao,Tang Chuanfang,Ma Xiaodong,Liu Zhihao,Peng Jinyong,Zhang Jianbin,Tang Zeyao
This work aimed to develop and optimize several lipid nanocapsule formulations (LNCs) to encapsulate cisplatin (CDDP) for treatment of hepatocellular carcinoma. By comparing the effect of oil/surfactant ratio, lecithin content, and oil/surfactant type on LNC characteristics, two LNCs were selected as optimal formulations: HS15-LNC (Solutol HS 15/MCT/lecithin, 54.5:42.5:3%, w / w ) and EL-LNC (Cremophor EL/MCT/lecithin, 54.5:42.5:3%, w / w ). Both LNCs could effectively encapsulate CDDP with the encapsulation efficiency of 73.48 and 78.84%. In vitro release study showed that both LNCs could sustain the release CDDP. Moreover, cellular uptake study showed that C6-labeled LNCs could be effectively internalized by HepG2 cells. Cellular cytotoxicity study revealed that both LNCs showed negligible cellular toxicity when their concentrations were below 313 μg/mL. Importantly, CDDP-loaded LNCs exhibited much stronger cell killing potency than free CDDP, with the IC50 values decreased from 17.93 to 3.53 and 5.16 μM after 72-h incubation. In addition, flow cytometric analysis showed that the percentage of apoptotic cells was significantly increased after treatment with LNCs. Therefore, the prepared LNC formulations exhibited promising anti-hepatocarcinoma effect, which could be beneficial to hepatocellular carcinoma therapy.
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