INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2024
·Vol. 25
·期 21
·11414
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IF 4.9
Rui-Yi Hu,Si-Min Qi,Ya-Jun Wang,Wen-Lin Li,Wan-Chen Zou,Zi Wang,Shen Ren,Wei Li
Age-related macular degeneration (AMD) is marked by a progressive loss of central vision and is the third leading cause of irreversible blindness worldwide. The exact mechanisms driving the progression of this macular degenerative condition remain elusive, and as of now, there are no available preventative measures for dry AMD. According to ancient records, ginseng affects the eyes by brightening them and enhancing wisdom. Modern pharmacological research shows that the active ingredients in ginseng, ginsenosides, may be used to prevent or improve eye diseases that threaten vision. Some articles have reported that ginsenoside Rg3 can treat diabetic retinopathy in mice, but no reports exist on its effects and mechanisms in AMD. Therefore, the role and mechanism of ginsenoside Rg3 in AMD warrant further study. This study aims to investigate the effects of Rg3 on AMD and its underlying molecular mechanisms. We established a mouse model of AMD to examine the impact of ginsenoside Rg3 on NaIO3-induced apoptosis in the retina and to explore the related intrinsic mechanisms. The in vivo results indicated that ginsenoside Rg3 prevents NaIO3-induced apoptosis in retinal pigment epithelial cells by inhibiting reactive oxygen species production and preventing the reduction in mitochondrial membrane potential. Additionally, we assessed the levels of protein expression within the apoptosis pathway. Ginsenoside Rg3 decreased the expression of Bax, cleaved caspase-3, and cleaved caspase-9 proteins. Additionally, it increased the expression of Bcl-2 by decreasing P-JNK levels. Moreover, our in vivo results showed that ginsenoside Rg3 enhanced retinal structure, increased the relative thickness of the retina, and decreased the extent of disorganization in both the inner and outer nuclear layers. Ginsenoside Rg3 may safeguard the retina against NaIO3-induced cell apoptosis by attenuating reactive-oxygen-species-mediated mitochondrial dysfunction, in which the JNK signaling pathway is also involved. These findings suggest that ginsenoside Rg3 has the potential to prevent or attenuate the progression of AMD and other retinal pathologies associated with NaIO3-mediated apoptosis.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Yuqing Sun,Chenye Dai,Jian Lu,Yapeng Zhu,Yingxuan Deng,Xianyin Cai,Dong Zou,Wenheng Jing
Oil droplets are inevitably deposited on the surface of membranes, resulting in membrane contamination during oily sewage separation. To effectively prevent oil fouling, a TiO 2 @MXene membrane with an ultra-glossy hydrophilic surface was designed via in-situ thermal induction to regulate the membrane surface structure and properties. Through high temperature-induced evolving the Ti valence and the functional groups of Ti 3 C 2 T x , uniform TiO 2 nanoparticles were generated in-situ on the Ti 3 C 2 T x surface while altering the surface structure and properties, which considerably improved the hydrophilicity, underwater oleophobic properties, and surface finish of the membrane. Specifically, the TiO 2 @MXene membrane (MT600) exhibited a low water contact angle of 7.5° and a high underwater oil contact angle of 133.3°. Notably, the oil adhesion force of the membrane was as low as 0.0013 μN, outperforming most oil water separation membranes. Thus, the unique structure and surface properties endowed MT600 membrane with excellent oil retention (TOC removal efficiency of > 95 %) and antipollution ability (permeance decay rates of < 10 %) for oil-in-water emulsion separation. This is because the ultra-glossy hydrophilic and oleophobic surface prompted the formation of a continuous and stable hydration layer on the membrane surface and prevented oil droplets from falling into the membrane grooves. Benefiting from the robust, ultra-glossy surface, the MT600 membrane exhibited excellent long-term durability and reusability (emulsion permeance recovery of > 90 %) in high-salt, highly acidic, and highly alkaline environments. This study opens pathways to realize ultra-glossy membrane surface for the efficient demulsification of small size emulsions and long-lasting, stable oil water separation.
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Ning Yu,Ting Wang,Cheng-Xu Xu,Xue Gou,Ying-Jiao Zhan,Wenli An,Zhi-Cheng Fu,Jinni Deng,Hai-Bo Zhao,Ming-Jun Chen
Rigid polyurethane foam (RPUF), a commonly employed thermal insulation material in buildings, is highly flammable, however, incorporating flame-retardant additives or fillers is significantly limited by their incompatibility, low effectiveness and negative impact on thermal insulation properties. Herein, a novel and simple interfacial bonding and in-situ packing strategy has been proposed to introduce self-intumescent biomass aerogel particles into RPUF. The result aerogel/RPUF composite manifested continuous and “sea-island” pore structures with a firm bonding interface between aerogel and RPUF due to the highly reactive activity between biomass chains (amino and hydroxyl groups) and isocyanate. Benefitting from the intricate thermal conductive paths and enhanced interface resistance, introducing aerogel particles can reduce the thermal conductivity from 36.5 mW·m −1 ·K −1 (RPUF) to 28.6 mW·m −1 ·K −1 , dropped by 21.6 %. Moreover, the aerogel/RPUF composite manifested excellent flame retardancy with a limiting oxygen index of 28 %, a significantly reduced peak heat release rate (−38.0 %) and smoke production (−54.0 %), as well as enhanced compressive strength (+93.7 %). This work provided new insight into fabricating RPUF composites with integrated advantages of high flame-retardant efficiency and outstanding thermal insulation.
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TRIBOLOGY LETTERS
·
2024
·Vol. 73
·期 1
·1-11
·
IF 2.9
Xu Wenya,Yang Guangbin,Zhang Shengmao,Zhang Yujuan,Fan Shuguang,Yu Laigui,Zhang Pingyu
Vegetable oil-based lubricants have a tendency to replace traditional petroleum-based lubricants due to their biodegradability, high flash point, low volatility, and low cost. However, polar molecules such as fatty acids in vegetable oil compete for adsorption with nanoparticles during rubbing process, resulting in imperfect tribological performance of nanoparticles. Magnetic nanoadditives can be adsorbed on the contact surface of iron-based friction materials through magnetic effects, which provides a new idea for solving competitive adsorption problems between additives and base oil. In this study, Ni nanoparticles with a particle size of approximately 15.6 nm were synthesized in-situ in olive oil using nickel acetylacetone as the nickel source and olive oil as the modifier and solvent required for the reaction, which is a simple, efficient, and environmentally friendly in-situ synthesis method. The as-synthesized Ni nanoparticles can significantly improve the antiwear capabilities of olive oil, reducing the wear scar diameter by 30%. The morphology and elemental analysis of wear scar indicated that a composite tribofilm including nickel, nickel oxide, iron oxide, carbon film, and polar fatty acid molecules in olive oil is formed on the rubbing surface, greatly improving the antiwear performance, which opens up an opportunity for the further application of new green nanolubricants.
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JOURNAL OF CATALYSIS
·
2024
·
IF 6.5
Longyu Wang,Haibo Jin,Zhichang Liu,Suohe Yang,Guangxiang He,Haiyan Liu,Xianghai Meng,Chunming Xu
The formation mechanism and pathway of HMF from fructose dehydration were investigated using in-line FTIR monitoring and in-situ 13 C NMR in this study. The results of the infrared absorption spectrum obtained at different time intervals during the reaction process showed significant inconsistencies with the acyclic pathway. However, the in-situ NMR analysis revealed the direct observation of two intermediates, fructose furan and (4R-5R)-4-hydroxy-5-hydroxymethyl-4,5-dihydrofuran-2-formaldehyde, providing direct evidence to support the cyclic carbocation pathway. This study also included theoretical analysis of the intermediates involved in the key steps of various reaction pathways using density functional theory (DFT) calculations. The key steps in the cyclic pathway were found to have lower activation energy compared to the acyclic pathway, while exhibiting significant differences in electronegativity at the reaction sites. The computational results not only reveal but also provide support for the rationality of the cyclic formation mechanism. The response surface methodology (RSM) was utilized to optimize the preparation process of HMF through fructose dehydration. It enabled determination of the significance order for each factor (reaction temperature > reaction time > mass percentage of water in the solvent) and identification of the optimal process conditions (reaction time of 3.5 h, reaction temperature of 143.5 °C, mass percentage of water in the solvent of 5.0 %, and maximum yield of 5-HMF reaching 82.4 %).
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Zhenxing Wang,Tingyang He,Yue Fang,Zengquan Lan,Benying Liu,Kin Weng Kong,Jian Sun,Xiahong He
This study systematically examined the effects of processing techniques on the flavor profiles and functional attributes of tea derived from fresh leaves ( Camellia sinensis ) of identical origin. Pu-erh raw tea (PRT), white tea (WT), and black tea (BT) were produced through distinct processing protocols (non-fermented, lightly fermented, and fully fermented, respectively). Antioxidant activity and sensory characteristics were evaluated alongside comprehensive metabolomic analyses using GC-IMS, GC–MS, and UHPLC-QTOF-MS. PRT exhibited superior antioxidant capacity with pronounced bitterness and astringency, whereas WT displayed fruity-sweet notes and BT demonstrated a mellow profile linked to fermentation. Metabolomic profiling identified six discriminative biomarkers and two pivotal compounds differentiating tea types, alongside six key metabolic pathways (e.g., secondary metabolite biosynthesis) driving compositional variations. These findings elucidate processing-induced biochemical transformations, offering insights for quality optimization and consumer-oriented tea selection.
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INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
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2024
·
IF 4.9
Haifeng Liu,Ying Zhang,Zeyong Zhong,Yanchun Gong,Pingting Yu,Yuhan Yang,Yichi Zhang,Tieli Zhou,Lijiang Chen
Colistin (COL) was once considered to be the last line of defence against multidrug-resistant bacteria belonging to the family Enterobacteriaceae . Due to the misuse of COL, COL-resistant (COL-R) Enterobacteriaceae have emerged. To address this clinical issue and combat COL resistance, novel approaches are urgently needed. In this study, the in vitro and in vivo antimicrobial and antibiofilm effects of the immunomodulator AS101 were investigated in combination with COL against COL-R Escherichia coli ( E. coli ) and Klebsiella pneumoniae ( K. pneumoniae ). Checkerboard assay, time-kill assay, and scanning electron microscopy confirmed the in vitro antimicrobial phenotype, whereas, crystal violet staining and multidimensional confocal laser scanning microscopy with live/dead staining confirmed the antibiofilm capability of the combination therapy. Moreover, the Galleria mellonella infection model and the mouse infection model indicated the high in vivo efficacy of the combination therapy. Additionally, cytotoxicity experiments performed using human kidney-derived HK-2 cells and hemolysis assays performed using human erythrocytes collectively demonstrated safety at effective combination concentrations. Furthermore, quantification of the expression of inflammatory cytokines via ELISA confirmed the anti-inflammatory advantage of combination therapy. At the mechanistic level, changes in outer and inner membrane permeability and accumulation of ROS levels, which might be potential mechanisms for synergistic antimicrobial effects. In conclusion, this study found that AS101 can restore COL susceptibility in clinical COL-R E. coli and K. pneumoniae and also has synergistic antibiofilm and anti-inflammatory capabilities. This study provided a novel strategy to combat clinical infections caused by COL-R E. coli and K. pneumoniae .
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2024
·
IF 4.8
Xing-Jie Zhang,Yu-Kun Pu,Peng-Yun Yang,Meng-Ru Wang,Rui-Han Zhang,Xiao-Li Li,Wei-Lie Xiao
Background NOD like receptor pyrin domain containing 3 (NLRP3) inflammasome is involved in innate immunity, and related to liver injury. However, no inflammasome inhibitors are clinically available until now. Our previous research suggests that isolicoflavonol (ILF), isolated from Macaranga indica , is a potent NLRP3 inflammasome inhibitor, but its mechanism is unclear. Methods Fluorescent imaging and Western blot assay were used to ascertain the effects of ILF on pyroptosis and NLRP3 inflammasome activation in macrophages. Next, Nrf2 signal pathway, its downstream gene transcription and expression were further investigated. ML385, a Nrf2 inhibitor, was used to verify whether ILF targets Nrf2 signaling. A carbon tetrachloride induced liver injury model was introduced to evaluate the liver protection activity of ILF in mice. Results This work revealed that ILF inhibited macrophage LDH release and IL-1β secretion in a dose-dependent manner. ILF had no significant cytotoxic effect on macrophage, it reduced pyroptosis and Gasdermin D N-terminal fragment formation. Moreover, ILF inhibited IL-1β maturation and Caspase-1 cleavage, but did not affect NLRP3, pro-Caspase-1, pro-IL-1β and ASC expression. ILF decreased ASC speck rate and reduced ASC oligomer formation. ILF decreased aggregated JC-1 formation restoring mitochondria membrane potential. In addition, ILF increased Nrf2 expression, extended Nrf2 lifespan and upregulated Nrf2 signaling pathway in macrophages whether the NLRP3 inflammasome was activated or not. Besides, ILF increased Nrf2 nuclear translocation, maintained a high proportion of Nrf2 in the nucleus, and upregulated ARE-related gene transcription and expression. Furthermore, Nrf2 signal inhibition attenuated compound ILF-mediated inhibition of pyroptosis, inflammasome activation and upregulation of Nrf2 signaling. ILF in a liver injury mouse model inhibited NLRP3 inflammasome activation and enhanced Nrf2 signaling. Conclusion Our study verified that ILF ameliorates liver injury via inhibiting NLRP3 inflammasome activation through boosting Nrf2 signaling, and highlighted that ILF is a potent anti-inflammatory drug for inflammasome-related liver diseases.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
·
IF 6.2
Cuiyuan Jin,Caiyun Wang,Chen Zhang,Qi Ye,Yuanxiang Jin,Liyun Shi
As a fungicide with the characteristics of high effectiveness, internal absorption and broad spectrum, imazalil is widely used to prevent and treat in fruits and vegetables. Here, pregnant C57BL/6 mice were exposed to imazalil at dietary levels of 0, 0.025‰, and 0.25‰ through drinking water during pregnancy and lactation. We then analyzed the phenotype, metabolome, and expression of related genes and proteins in the livers of mice. There was a marked decrease in the body and liver weights of male offspring mice after maternal imazalil exposure, while this effect on the dam and female offspring was slight. Metabolomics analyses revealed that imazalil significantly altered the metabolite composition of liver samples from both dams and offspring. The preliminary results of the analysis indicated that glucolipid metabolism was the pathway most significantly affected by imazalil. We performed a coabundance association analysis of metabolites with significant changes in the pathway of glycolipid metabolism, and IMZ altered the networks of both dams and offspring compared with the network in control mice, especially in male offspring. The hepatic triglyceride, non-esterified fatty acid and glucose levels were increased significantly in the dams but decreased significantly in male offspring after maternal imazalil exposure. Furthermore, the expression levels of genes associated with glycolipid metabolism and m 6 A RNA methylation were significantly affected by maternal intake of imazalil. Imazalil-induced glucolipid metabolism disturbance was highly correlated with m 6 A RNA methylation. In conclusion, maternal imazalil exposure resulted in glucolipid metabolism disturbance and abnormal m 6 A RNA methylation in the livers of dams and offspring mice. We expected that the information acquired in this study will provide novel evidence for understanding the effect of maternal imazalil exposure on potential health risks.
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MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Wen Sun,Qi Yuan,Mingyue Tan
Nanotechnology integration into agricultural science and technology has led to the development of nano-pesticides, which are pivotal in enhancing the efficacy of pest control and reducing the environmental footprint of traditional pesticides. However, there is a significant knowledge gap regarding the absorption, transportation, and distribution of nanoparticle carriers within crops, which is essential for the safe and effective application of these nano-pesticides. Taking advantage of high sensitivity and minimal interference from biological backgrounds, herein, surface-enhanced Raman scattering (SERS) labeling technology was applied to track the in vivo distribution of nano-pesticide carriers in plant. AuNS@Cy7@TiO 2 model NPs with distinct near-infrared SERS signals were synthesized applied to ginseng leaves to monitor their behavior. SERS imaging results demonstrate that these NPs can migrate from treated leaflets to adjacent leaflets and petioles, penetrating the mesophyll tissue and distributing uniformly. Importantly, the absence of SERS signals in the aerial stem indicates that the NPs do not translocate to the roots, thus posing minimal risk to the medicinal parts of ginseng. The presence of the surfactant Silwet L-77 increases the migration distance of nanoparticles within the leaves. This study highlights the potential of SERS labeling for tracking NP movement in plants, providing insights into the absorption and transport mechanisms of nanocarriers, and guiding the development of safer and more effective nano-pesticides.
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Pharmaceutics
·
2024
·Vol. 16
·期 6
·805
·
IF 4.9
Ailing Wu,Houyin Shi,Luhan Yang,Hao Zhang,Xichen Nan,Dan Zhang,Zhuo Zhang,Chun Zhang,Siwei Chen,Xiujuan Fu,Lilan Ou,Lulu Wang,Yanyan Shi,Hao Liu
Etomidate is a general anesthetic that has shown good hemodynamic stability without significant cardiovascular or respiratory depression. Despite several kinds of dosage forms having been reported for this drug, formulation types are very limited in clinical practice, and brain-targeted formulations for this central nervous system (CNS) drug have been rarely reported. Moreover, studies on the biocompatibility, toxicity, and anesthetic effects of the etomidate preparations in vivo were inadequate. The present study was to develop lactoferrin-modified liposomal etomidate (Eto-lip-LF) for enhanced drug distribution in the brain and improved anesthetic effects. Eto-lip-LF had good stability for storage and hemocompatibility for intravenous injection. Compared with the non-lactoferrin-containing liposomes, the lactoferrin-modified liposomes had notably enhanced brain-targeting ability in vivo, which was probably realized by the binding of transferrin with the transferrin and lactoferrin receptors highly distributed in the brain. Eto-lip-LF had a therapeutic index of about 25.3, higher than that of many other general anesthetics. Moreover, compared with the commercial etomidate emulsion, Eto-lip-LF could better achieve rapid onset of general anesthesia and rapid recovery from anesthesia, probably due to the enhanced drug delivery to the brain. The above results demonstrated the potential of this lactoferrin-modified liposomal etomidate to become an alternative preparation for clinical general anesthesia.
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BIOORGANIC CHEMISTRY
·
2024
·
IF 4.5
Haitao Zhu,Houcong Li,Yuxin Ji,Min Hou,Qingling Yang,Lili Liang,Wenge Li
A non-platinum-metal decanuclear complex [Ni 10 L 4 (CH 3 COO) 8 (C 2 H 5 OH) 8 ]·8(C 2 H 5 OH) ( Ni 10 complex) has been developed with a tri-dentate 2,3-dihydroxybenzaldehyde-2-aminophenol Schiff base ligand (H 3 L). Single crystal X-ray analysis reveals that the Ni 10 complex displays a sandwich loaf-shaped decanuclear structure and its anticancer activity was evaluated. The cell cytotoxicity results indicating that the Ni 10 complex is most effective to human breast cancer cells MDA-MB-231 and its mechanism were further investigated. Flow cytometry analysis showed that the Ni 10 complex triggered cell cycle arrest and induced apoptosis of MDA-MB-231 cells. Western blot analysis of the changes of intracellular protein expression showed that Ni 10 triggers MDA-MB-231 apoptosis through mitochondrial mediated apoptosis signaling pathways. In vivo experiments showed that the Ni 10 complex significantly suppressed breast tumor growth with low toxicity against major organs in a nude mice model. The good treatment effect, low toxicity and pharmacological mechanisms of the decanuclear Ni II complex may provide a clue for the research and development of non-platinum multinuclear based chemotherapeutic drugs.
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