JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2021
·Vol. 25
·期 3
·1073-1081
·
IF 2.647
Jin Xin,Lv Mengmeng,Pan Qing,Fang Shu,Zhu Ningning
We describe a label-free electrochemical aptasensor for adenosine triphosphate (ATP) assay based on bifunctional Fe 3 O 4 @Au nanocomposites. Due to the introduction of Au nanoparticles, the resulting bifunctional Fe 3 O 4 @Au nanocomposites can be easily modified with ATP-specific aptamer molecules through Au–S interaction to build the nanoprobe and used to immobilize on a glassy carbon electrode simply with the assistance of a magnet. Once exposed to the target ATP, the current signal obtained at nanoprobe-containing magnetic glassy carbon electrode decreases immediately. The decrease in sensor current is a result of the less methylene blue adsorbed on the aptamer caused by the fact that the ATP-specific aptamer prefers to bind with ATP rather than with methylene blue. The decrease in sensor current can be used for electrochemical determination of ATP, for example, to determine ATP in human serum samples.
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MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 12
·3201-3208
·
IF 4.831
Lan Jianming,Li Li,Liu Yingxin,Yan Liu,Li Chunyan,Chen Jinghua,Chen Xiaosong
Upconversion nanoparticles (UCNPs) of the type α-NaYF 4 :Yb 3+ , Er 3+ and a typical diameter of 6–7 nm were synthesized by thermal decomposition of the respective rare-earth stearate. The oleic acid on the surface of the UCNPs was then replaced by aptamer DNA. The assay was performed in a microplate format with a capture probe immobilized in the wells. Following binding of the vascular endothelial growth factor (VEGF), an auxiliary probe DNA is added that is labeled with UCNPs and binds to the VEGF-loaded capture probe. The method enables highly sensitive and highly specific detection of the VEGF which is a marker for breast cancer. Under the optimum conditions, the intensity of the upconversion luminescence (at excitation/emission wavelengths of 980/541 nm) is linearly proportional to the VEGF concentration in the 50 pM to 2000 pM concentration range, with a 6 pM detection limit. The method was applied to the determination of VEGF in spiked serum, typically at a 500 pM level, and gave recoveries that ranged from 98 to 113 %, with RSDs between 2.9 and 3.6 %. This makes it a viable tool for early diagnosis of breast cancer.
An upconversion luminescence based assay is presented for trace analysis of vascular endothelial growth factor. It is based on a sequence fragment-linked technique of target-induced aptamer (AP) and upconversion nanoparticles (UCNPs) and has potential application in the early diagnosis of breast cancer.
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Nature Communications
·
2025
·
IF 15.7
Yang Wan,Pan Tengfei,Yang Guang,Zhu Jianbing,Ran Xueqin,Ren Biyun,Zuo Mengnan,Chao Lingfeng,Xia Yingdong,Chen Yonghua
Interfacial dipolar molecules play a crucial role in achieving high-performance perovskite solar cells (PSCs). However, the random distribution at the interface often limits their ability to effectively regulate interfacial energy levels and carrier extraction. Here, we report a bifunctional antisymmetric molecule, N-(1-carboxyethyl)iminodiacetic acid trisodium salt (MGDA·3Na), which enables both directional alignment and stable anchoring on SnO 2 and perovskite interfaces. The methyl side chain in the MGDA - anion induces intramolecular electronic polarization and dipole reorientation, facilitating selective adsorption on the SnO 2 surface and perovskite buried interface. This significantly improves interface uniformity, enabling efficient passivation of SnO 2 surface defects and precise regulation of perovskite crystal growth. The dual anchoring effect not only aligns energy levels to minimize charge extraction barriers but also templates vertical perovskite growth, reducing buried interface defects and enhancing crystallization quality. As a result, we achieved a power conversion efficiency of 26.43% in small-area PSCs and 23.27% in a 5 × 5 cm 2 large-area module, highlighting the potential for industrial-scale application. The device maintains 96% of its initial efficiency for 2000 h under nitrogen-filled glove box, 96% after 800 h at 55 °C/55% RH, and 99% after 800 h of continuous illumination at the maximum power point.
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Nature Communications
·
2025
·
IF 15.7
Jin Chengkang,Xu Xiaoling,Yao Ning,Zhang Hairui,Chen Changjiang,Zeng Lingyao,Liu Zhiyun,Lei Lanjie,Cui Shumao,Wen Chengping,Shi Liyun
Accumulation of senescent cells is associated with aging and age-related diseases. However, current clearance therapies targeting senescent cells are often limited by low efficiency, poor specificity, and insufficient penetration. Here we develop a nano-platform composed of a probe (GD) that can be specifically activated by senescent cells, a photosensitizer (Ce6), and a peptide (HK) for targeting ferritin, named HK-PCGC. We show that upon entering senescent cells, GD is activated by high levels of β-galactosidase, releasing fluorescence to excite Ce6. Ce6 then generates reactive oxygen species to eliminate these cells. Additionally, we find that under the guidance of the peptide HK, our system degrades ferritin to trigger ferroptosis, further eliminating senescent cells. Collectively, we demonstrate that HK-PCGC can effectively eliminate senescent cells, reduce the senescence-associated secretory phenotype, and safely improve the physical fitness of aged mice. This study integrates senescent cell responsiveness, laser-free photodynamic therapy, and induction of ferroptosis, offering a potential approach for delaying aging.
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JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
·
2025
·Vol. 44
·期 1
·321
·
IF 12.8
Lei Yu,Bai Yaowei,Bai Xiatong,Sun Bo,Zhu Licheng,Wu Wenlong,Su Yang,Zhang Hongsen,Wang Yingliang,Zheng Chuansheng
Background and aims Radiofrequency ablation (RFA) is an effective treatment for hepatocellular carcinoma (HCC), but incomplete ablation and recurrence of residual tumors remain significant challenges, partly due to local inflammation and elevated COX-2 levels in the tumor microenvironment. This study aims to investigate the potential of combining celecoxib, a COX-2 inhibitor, with anti-PD-1 monoclonal antibody (αPD-1) to enhance anti-tumor efficacy and activate immune responses. Methods In vitro, ELISA was used to assess the effects of radiofrequency heat treatment and celecoxib on PGE 2 secretion by Hepa1-6 cells. Scanning electron microscopy, flow cytometry, and CCK-8 assays were employed to evaluate the function and cytotoxic activity of NK92 cells against Hepa1-6 cells. In vivo, orthotopic HCC mice were divided into five groups to evaluate tumor volume, pathology, and survival. The role of celecoxib in the COX-2/PGE 2 /NK cell axis and its impact on NK cell immune function were investigated. Results In vitro experiments showed that celecoxib reversed PGE 2 -mediated suppression of NK cell function and cytotoxic activity against HCC cells by inhibiting PGE 2 secretion. In vivo, orthotopic HCC mice treated with celecoxib and αPD-1 exhibited significantly reduced tumor volumes, attenuated the infiltration and activation of NK cells, and prolonged survival compared to control groups. The combination therapy also demonstrated a notable abscopal-like effect, inhibiting metastatic tumor growth and activating systemic immunity. Conclusions Taken together, these findings suggest that celecoxib combined with αPD-1 represents a promising strategy for treating residual HCC after RFA, with enhanced anti-tumor effects and good safety. Graphical abstract
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Chemical and Biological Technologies in Agriculture
·
2025
·Vol. 12
·期 1
·176
·
IF 5.2
Li Fusen,Li Ping,Wang Xinping,Gu Yinghui,Luan Zhihui,Song Kai
Background Selenium (Se) deficiency affects a significant portion of the global population, making biofortification of staple crops essential for public health. While selenium nanoparticles (SeNPs) show promise for biofortification due to their low toxicity and high bioavailability, their application is limited by poor colloidal stability. This study explores the potential of Phellinus igniarius polysaccharide-stabilized SeNPs (SHP–SeNPs) to enhance rice growth and selenium biofortification (Se biofortification), focusing on their underlying molecular mechanisms. Results SHP–SeNPs demonstrated excellent colloidal stability and significantly improved rice physiological parameters. A foliar application of 45 mg/L SHP–SeNPs increased chlorophyll content, soluble sugars, and soluble proteins, surpassing the effects of unmodified selenium nanoparticles (SeNPs) and SHP alone. Field trials confirmed that SHP–SeNPs boosted rice grain yield and achieved Se biofortification, with polished rice containing 0.228 mg/kg Se, primarily as bioavailable selenomethionine. Metabolomics analysis revealed that SHP–SeNPs activated metabolic pathways such as the TCA cycle and sugar metabolism, upregulating 16 core metabolites including flavonoids, terpenoids, and glycerophospholipids, which contributed to the observed growth promotion. Conclusions SHP–SeNPs enhance rice growth, biofortification, and overall metabolic activity through a synergistic effect between SHP and SeNPs. The results highlight the potential of SHP–SeNPs as a stable and effective agent for Se biofortification in rice. This work provides valuable insights into the metabolic reprogramming of plants induced byfunctionalized nanoparticles and lays the foundation for developing intelligent nano-fertilizers to improve crop nutrition. Graphical
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MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·44
·
IF 5.3
Fan Yafeng,Jiao Baoping,Yu Zhongping,Zong Bingbing,Li Haiyan,Wu Xianzhen
A new kind of electrochemical biosensor was fabricated by modifying a glassy carbon electrode (GCE) with Ni-doped ZnO nanorods suspension for the sensitive and selective detection of miRNA-21. The electrode surface was coated with polydopamine (PDA), crosslinked with glutaraldehyde, and functionalized with DNA probes. Bovine serum albumin (BSA) was then used to block non-specific binding sites. Electrochemical analyses were adopted to systematically investigate the progress of the stepwise electrode modification and the hybridization. The electrochemical characterization indicated a gradual decrease in current response and an increase in charge-transfer resistance after each modification step, verifying the successful assembly of the sensing interface. Following optimization, the biosensor could detect concentrations as low as 21.30 fM with a dynamic linear range of 10 − 6 nM to 10 5 nM, good reproducibility, and selectivity to the mismatch and non-complementary sequences. Crucially, recovery experiments in spiked human serum samples gave satisfactory results, confirming the clinical potential of the system. In general, the Ni-doped ZnO nanorod-based biosensor provides sensitive, label-free, and low-cost detection of circulating miRNAs. Its modularity allows for simple conversion for detection of other nucleic acid-based biomarkers, implicating it as a useful diagnostic tool for early cancer diagnosis and point-of-care screening. Graphical abstract
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MOLECULAR NEUROBIOLOGY
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2025
·Vol. 63
·期 1
·229
·
IF 4.3
Zhang Zhiyang,Xu Nixi,Tao Zean,Liang Yun,Jin Lixia,Li Zhuoxuan,Cao Yuanwu,Jiang Chang,Chen Zixian
Spinal cord injury (SCI) often results in permanent functional loss due to the complexity of the post-SCI microenvironment, but the composition and functional dynamics of the post-SCI microenvironment remain poorly understood. We applied spatial metabolomics to detect metabolites within the spinal cord of SCI rats, revealing the existence of a high-uric acid (hUA) microenvironment. Subsequently, we utilized Bulk-RNA sequencing and single-cell RNA sequencing (scRNA-seq) analysis, identified elevated expression of Cellular communication network factor 1 (Ccn1) and alterations in purine metabolic patterns. To investigate the role of Ccn1 within this hUA microenvironment, PC12 cell line and Sprague–Dawley (SD) rats were used. In vitro , 4 groups were designed: Control ( n = 6), UA (PC12 cells treated with 200 μM UA for 24 h) ( n = 6), siCcn1 ( Ccn1 knockdown using small interfering RNA) ( n = 6), and siCcn1 + UA ( n = 6). In vivo groups: SCI (spinal cord injury only) ( n = 6), hUA (induced hUA microenvironment by gavage with oxonic acid potassium salt 750 mg/kg and and ethambutol 250 mg/kg) ( n = 6), AAV ( Ccn1 knockdown via adeno-associated virus) ( n = 6), and AAV + hUA ( n = 6). Results showed that UA significantly reduced cellular aerobic respiration levels (Control vs UA: 62.87 ± 16.76 vs 17.09 ± 4.91 pmol/min, P < 0.01) and induced pyroptosis, Ccn1 knockdown significantly improved aerobic respiration ( siCcn1 + UA vs UA: 46.45 ± 12.54 vs 17.09 ± 4.91 pmol/min, P < 0.05) and reduced the level of pyroptosis. Total cellular ATP levels, the NAD⁺/NADH ratio, and proliferation capacity were also assessed. In vivo , Ccn1 knockdown significantly increased the Basso, Beattie, and Bresnahan (BBB) locomotor scores of animals on the 7 days post-injury ( AAV + hUA vs hUA: 3.33 ± 0.52 vs 2.00 ± 0.63, P < 0.05) and reduced neuronal pyroptosis. The hUA microenvironment upregulated Ccn1 expression, suppressed aerobic respiration, induced pyroptosis, and disrupted purine metabolism, Ccn1 knockdown reversed these biological processes, suggesting its potential as a therapeutic target for SCI.
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Nature Communications
·
2025
·
IF 15.7
Yao Yuan,Zhang Xinyue,Cao Yingying,Liu Long,Zhang Yanqiang,Zhang Suojiang
Cyclopropane-based aerospace fuels exhibit the excellent volumetric calorific value, leading to broad application prospects in aircrafts. However, due to the highly rigid structure of the cyclopropane (compared to cyclobutane or cyclopentane, etc.) and the difficulty in forming metal-carbene intermediates, there are still significant challenges in the precise synthesis of multi-cyclopropane-based high-energy fuels ( ≥ 2 cyclopropane structures). Herein, via the introduction of bending strain on metal organic complex, we report a single-site Cu/single-walled carbon nanotube catalyst (Cu/SWCNT), which possesses 2.6-fold conversion (up to 77.4%) and 4-fold selectivity (up to 45.7%) as that without bending strain for the preparation of bi-cyclopropane-based fuels from dienes. XAFS and DFT calculations show that bending strain enhances the upward shift of d-band center of β electrons (−4.370 to −4.366), thus promotes the adsorption and activation of CH 2 N 2 and alkenes. Moreover, bending strain elongates Cu-O bonds (1.91 to 1.96 Å), making it easier for CH 2 N 2 to insert and form Fischer carbene intermediates. These two aspects obviously reduce the reaction energy barrier from 40.3 to 33.7 kcal·mol -1 for the cyclopropanation process. Our work provides a method of strain engineering to regulate C-C coupling reactions at the molecular level, and achieve highly efficient cyclopropanation from alkenes.
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VETERINARY RESEARCH
·
2025
·
IF 3.5
Su Kai,Ren Jing,Zhang Yanan,Yuan Chen,Wang Yawen,Yang Liu,Fu Lizhi,Fan Tingli,Song Qinye
Porcine epidemic diarrhea (PED), a highly contagious intestinal disease caused by PEDV, threatens newborn piglets and causes enormous losses in the swine industry. Intestinal mucosal immunity is vital for defense against PEDV. Chitosan (CS), a mucin-adhesive biodegradable polysaccharide, has emerged as a promising mucosal vaccine delivery vector and antigen adjuvant. To develop a PEDV mucosal vaccine, we prepared S1-CS nanoparticles (NPs) loaded with the S1 domain of the PEDV spike (S) protein. We evaluated their ability to induce mucosal immune responses in mice via different inoculation routes and to confer immune protection in piglets after oral inoculation. The results showed that S1-CS NPs exhibited no cytotoxicity and remained stable in simulated gastric fluid in vitro. Compared with oral administration, intramuscular inoculation of S1-CS NPs induced higher levels of PEDV-specific serum IgG in mice. However, PEDV-specific IgA was detected only in the serum and intestinal lavage fluid of orally vaccinated mice. Oral administration also elicited higher IFN-γ levels than intramuscular injection, suggesting stronger activation of cellular immunity. In piglets, oral S1-CS NPs induced serum neutralizing antibodies and IgA responses in both serum and intestinal mucosa, increased the number of IgA-secreting cells in the intestine, reduced viremia and virus shedding, and improved intestinal villus morphology. These findings indicate that the S1-CS NPs developed in this study represent promising candidates for an oral mucosal PEDV vaccine.
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Cell Death Discovery
·
2025
·
IF 7
Rui Ma,Chen Peng,Wenjuan Chen,Yizhen Zhang,Yangfeng Ding,Xin Wang,Yuling Shi
The skin barrier is crucial for protecting against environmental challenges, preventing water loss, and regulating immune responses. This study aims to investigate the roles and mechanisms of SERPINB7 in skin barrier maintenance. We found that SERPINB7 deficiency disrupts tight junctions of keratinocytes in vitro, and specific knockout of Serpinb7 in keratinocytes impairs skin barrier function in vivo. SERPINB7 deficiency leads to reduced expression of O-GalNAc regulatory proteins and structural abnormalities in the Golgi apparatus, ultimately impairing protein O-GalNAc glycosylation. Legumain acts as a critical mediator in the maintenance of normal biological functions and O-GalNAc glycosylation regulated by SERPINB7. O-GalNAc inhibition exhibits biological effects analogous to those induced by SERPINB7 deficiency, leading to weakened tight junctions, reduced cell adhesion, and compromised skin barrier integrity in keratinocytes and mouse skin, respectively. Consequently, O-GalNAc deficiency exacerbates inflammatory skin diseases such as psoriasis and atopic dermatitis. Mechanistically, O-GalNAc deficiency primarily affects the glycosylation of calcium-related and cell adhesion-related proteins, disrupting calcium signaling and compromising cell adhesion, ultimately leading to skin barrier dysfunction. In summary, this study demonstrates that SERPINB7 maintains skin barrier through protein O-GalNAc glycosylation. These findings not only deepen our understanding of skin barrier biology but also provide new insights for developing therapeutic strategies for skin barrier-related diseases.
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WATER AIR AND SOIL POLLUTION
·
2025
·Vol. 237
·期 5
·317
·
IF 3
Wang Kexun,Wang Yichen,Zhu Xueqiang,Zhou Lai
This study investigates the in situ degradation of naphthalene (NAP) in contaminated soil using a biochar-supported nano zero-valent iron (BC@nZVI) composite to activate persulfate (PS). NAP, a persistent polycyclic aromatic hydrocarbon (PAH), poses significant ecological risks due to its carcinogenicity and environmental mobility. The BC@nZVI composite was synthesized via a two-step hydrothermal–carbonization method and characterized using XRD, SEM, TEM, BET, and FT-IR, confirming its enhanced stability and reduced aggregation compared to bare nZVI. Sandbox experiments optimized critical injection parameters, demonstrating that a PS dosage of 2% soil mass and a BC@nZVI:PS mass ratio of 1:2 achieved 76.6% NAP degradation within 4.5 cm of the injection well after 72 h. Pre-injection of BC@nZVI followed by PS at a high flow rate (2.68 L·min⁻ 1 ) enhanced reagent distribution, yielding degradation rates of 44.1–80.3% across a 9 cm radius. Reduced injection volumes (3.2 L) or reversed injection sequences diminished efficiency by 14–25%, while excessive reagents induced radical self-quenching (e.g., SO 4 − · scavenging) and Fe 2 ⁺ overconsumption. Compared to conventional Fe 2 ⁺-activated systems, the BC@nZVI/PS system exhibited superior soil penetration, sustained radical generation, and reduced PS consumption, effectively addressing challenges in heterogeneous soil remediation. These findings provide actionable strategies for scalable PAH-contaminated site remediation, balancing high efficacy with operational and environmental feasibility. Graphical
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