Chinese Medicine
·
2021
·Vol. 16
·期 1
·1-14
·
IF 5.455
Yu Yunru,Chen Jing,Zhang Xiaohui,Wang Yingchao,Wang Shufang,Zhao Lu,Wang Yi
Background Inflammatory bowel diseases (IBD) are chronic relapsing intestinal inflammations with increasing global incidence, and new drug development remains in urgent demand for IBD management. To identify effective traditional Chinese medicine (TCM) formulae and compounds in IBD treatment, we innovatively combined the techniques of knowledge mining, high-content screening and high-resolution mass spectrometry, to conduct a systematic screening in Zhongjing formulae, which is a large collection of TCM prescriptions with most abundant clinical evidences. Methods Using Word2vec-based text learning, the correlations between 248 Zhongjing formulae and IBD typical symptoms were analyzed. Next, from the top three formulae with predicted relationship with IBD, TCM fractions were prepared and screened on a transgenic zebrafish IBD model for their therapeutic effects. Subsequently, the chemical compositions of the fraction hits were analyzed by mass spectrometry, and the major compounds were further studied for their anti-IBD effects and potential mechanisms. Results Through knowledge mining, Peach Blossom Decoction, Pulsatilla Decoction, and Gegen Qinlian Decoction were predicted to be the three Zhongjing formulae mostly related to symptoms typical of IBD. Seventy-four fractions were prepared from the three formulae and screened in TNBS-induced zebrafish IBD model by high-content analysis, with the inhibition on the intestinal neutrophil accumulation and ROS level quantified as the screening criteria. Six herbal fractions showed significant effects on both pathological processes, which were subsequently analyzed by mass spectrometry to determine their chemical composition. Based on the major compounds identified by mass spectrometry, a second-round screen was conducted and six compounds (palmatine, daidzin, oroxyloside, chlorogenic acid, baicalin, aesculin) showed strong inhibitory effects on the intestinal inflammation phenotypes. The expression of multiple inflammatory factors, including il1β , clcx8a , mmp and tnfα , were increased in TNBS-treated fish, which were variously inhibited by the compounds, with aesculin showing the most potent effects. Moreover, aesculin and daidzin also upregulated e-cadherin’s expression. Conclusion Taken together, we demonstrated the regulatory effects of several TCM formulae and their active compounds in the treatment of IBD, through a highly efficient research strategy, which can be applied in the discovery of effective TCM formulae and components in other diseases. Graphic abstract
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PubMed
高影响因子
PARASITOLOGY RESEARCH
·
2019
·Vol. 118
·期 4
·1239-1248
·
IF 2.067
Chen Jinglong,Ma Fuli,Li Xiangrui,Zhao Ruqian,Yang Xiaojing
Haemonchus contortus is a highly pathogenic gastrointestinal nematode of small ruminant animals. In modern intensive farming, livestock often suffer from different types of stress. However, whether host stress hormones influence H. contortus infection is largely unknown. Therefore, we treated H. contortus with norepinephrine (NE) and analyzed the changes in its excretory/secretory products (ESPs). Label-free quantitative proteomic analysis was used to identify differences in body proteins and ESPs between the control and NE-treated groups. We also investigated the changes in ESP action by analyzing cytokine secretion and goat peripheral blood mononuclear cell (PBMC) proliferation after incubation with ESPs secreted by NE-treated H. contortus . Thirty-two proteins in the body samples and 137 in the ESPs were differentially expressed between the groups. Gene ontology (GO) annotation showed that the functions of these different proteins might be involved in energy metabolism, protein metabolism, lipid metabolism, redox homeostasis, ion channel, and cell structure. NE treatment caused oxidative stress in H. contortus and changed the expression levels of some immunogenic proteins, such as the 15-kDa ESP . Meanwhile, the ESPs secreted by NE-treated H. contortus significantly decreased PBMC proliferation and the interleukin (IL)-2, IL-4, and interferon-gamma contents. Thus, NE treatment significantly affected the H. contortus body and ESP expression, and changes in the ESPs influenced PBMC function. The results reveal a relationship between host hormones and parasites and provide new clues to explain some of the variation in individual responses to infection.
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PubMed
高影响因子
NEUROCHEMICAL RESEARCH
·
2018
·Vol. 43
·期 12
·2435-2445
·
IF 2.772
Zhang Man,Huang Li-Li,Teng Chen-Huai,Wu Fang-Fang,Ge Li-yun,Shi Yu-Juan,He Zheng-Le,Liu Lei,Jiang Cheng-Jie,Hou Ruo-Nan,Xiao Jian,Zhang Hong-Yu,Chen Da-Qing
Traumatic brain injury (TBI) is a serious public health and medical problem worldwide. Oxidative stress plays a vital role in the pathogenesis of TBI. Nuclear factor erythroid 2-related factor 2 (Nrf2), an important factor in the cellular defense against oxidative stress, is activated following TBI. In this study, the protective effects of Isoliquiritigenin (ILG), a promising antioxidant stress drug, was evaluated as a protective agent against TBI. In a mouse model of controlled cortical impact Injury, we found that the ILG administration reduced the Garcia neuroscore, injury histopathology, brain water content, cerebral vascular permeability, the expression of cleaved caspase3, aquaporin-4, glial fibrillary acidic protein and the increased the expression of neurofilament light chain protein, indicating the protective effects against TBI in vivo. ILG treatment after TBI also restored the oxidative stress and promoted the Nrf2 protein transfer from the cytoplasm to the nucleus. We then used Nrf2−/− mice to test the protective effect of Nrf2 during ILG treatment of TBI. Our findings indicated that Nrf2−/− mice had greater brain injury and oxidative stress than wild-type (WT) mice and ILG was less effective at inhibiting oxidative stress and repairing the brain injury than in the WT mice. In vitro studies in SY5Y cells under oxygen glucose deprivation/re-oxygenation stimulation yielded results that were consistent with those obtained in vivo showing that ILG promotes Nrf2 protein transfer from the cytoplasm to the nucleus. Taken together, our findings demonstrate that Nrf2 is an important protective factor against TBI-induced injuries, which indicates that the protective effects of ILG are mediated by inhibiting oxidative stress after TBI via a mechanism that involves the promotion of Nrf2 protein transfer from the cytoplasm to the nucleus.
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PubMed
高影响因子
MOLECULAR NEUROBIOLOGY
·
2026
·Vol. 63
·期 1
·956
·
IF 5.5
Cao Yuting,Tang Peipei,Feng Dan,He Hankuan,Wu Han,Liu Yang,Tian Jinfeng,Hu Kongzhen,Zhong Yuhua
Fibrotic scar formation after ischemic stroke limits neuroplasticity and functional recovery. While estradiol (E2) exerts anti-fibrotic effects, the mechanisms by which high-frequency repetitive transcranial magnetic stimulation (rTMS) promotes post-stroke recovery remain poorly understood. We investigated whether rTMS attenuates post-ischemic brain fibrosis via aromatase-dependent local E2 synthesis. Male Sprague–Dawley rats subjected to permanent middle cerebral artery occlusion received high-frequency rTMS starting post-stroke day 3. Fibrosis and metabolism were evaluated using longitudinal dual-tracer [ 18 F]AlF-FAPI-42 and [ 18 F]FDG PET/CT on days 3 and 14, alongside histological and biochemical assessments. The aromatase inhibitor letrozole was used to probe mechanistic dependence. Additionally, we retrospectively analyzed dual-tracer PET/CT data from 10 ischemic stroke patients. In rats, rTMS significantly reduced [ 18 F]AlF-FAPI-42 uptake, decreased collagen deposition and fibrotic marker expression, increased [ 18 F]FDG uptake, and improved neurological outcomes. rTMS also upregulated aromatase expression and elevated peri-infarct E2 levels; these anti-fibrotic and metabolic benefits were no longer observed under letrozole treatment. Brain E2 levels correlated negatively with [ 18 F]AlF-FAPI-42 uptake and positively with motor performance. Corroborating the preclinical model, clinical imaging revealed elevated [ 18 F]AlF-FAPI-42 uptake in the ischemic hemisphere of patients, which was inversely associated with [ 18 F]FDG uptake. These findings demonstrate that high-frequency rTMS attenuates post-stroke fibrosis and promotes metabolic recovery partly through aromatase-dependent elevation of local E2. Furthermore, dual-tracer [ 18 F]AlF-FAPI-42/[ 18 F]FDG PET provides a novel, non-invasive method for monitoring fibrosis–metabolism coupling. The clinical findings highlight the translational relevance of this relationship, suggesting that tracking fibrotic remodeling could guide post-stroke rehabilitation.
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高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2026
·
IF 3.4
Li Ya,Lu Mingfei,Pan Yuhua,Liang Jianfeng
In order to investigate characteristic marker metabolites and their grade-dependent variations in Liupao tea, non-volatile compounds from four grades of Liupao tea were analysed by combining sensory evaluation with UPLC-Q-TOF-MS based untargeted metabolomics. Sensory evaluation revealed that decreasing Liupao tea grade was accompanied by reduced stale flavour, gradual increases in bitterness and astringency, and an overall deterioration of sensory quality. In total, 4 099 non-volatile compounds were detected, of which 181 metabolites were common across all four grades. 14 significantly differential metabolites, including EC, EGC and L-theanine, displayed regular grade-related changes with Pearson correlation coefficients of |r| ≥ 0.7. Multivariate statistical analysis confirmed strong correlations between these 14 key compounds and both tea grade and sensory attributes. A PLS regression model built upon the 14 markers achieved an internal fitting R²of 0.9929. It should be noted that this model was trained solely on samples originating from a single tea garden, multi-sample external validation is required to verify its generalisability. This study provides theoretical references for unravelling flavour differences among differently graded Liupao tea.
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高影响因子
INTERNATIONAL JOURNAL OF IMPOTENCE RESEARCH
·
2026
·
IF 2.5
Gu Wenhui,Wu Lingyu,Yang Zhiguo,Cheng Zhongbin,Liu Wenwen,Huang Yi-You,Liu Dan,An Faliang,Li Jian,Zhou Qian,Li Baoli,Luo Hai-Bin
Wendlandia uvariifolia subsp. chinensis (Merr.) Cowan is a traditional medicinal plant used by the Li ethnic group in Hainan Province, China, for the treatment of herpes zoster. However, its pharmacological properties remain unclear. This study aimed to evaluate its phosphodiesterase type 5 inhibitory activity, identify the active components, and explore its therapeutic potential for erectile dysfunction. Through high-throughput screening of 1200 traditional Chinese herbal medicines, this plant was identified as a potential phosphodiesterase type 5 inhibitor. Bioactivity-guided fractionation and gas chromatography–mass spectrometry analysis identified the petroleum ether fraction as the most active fraction, while phytol, one of its identified constituents, inhibited phosphodiesterase type 5 in vitro with an IC₅₀ value of 2.5 ± 0.1 μmol/L. In H₂O₂-injured endothelial cells, petroleum ether fraction significantly restored nitric oxide production. In a paroxetine-induced erectile dysfunction rat model, the fraction improved sexual behavior parameters, restored NO/cGMP signaling, and alleviated pathological remodeling and fibrosis in the corpus cavernosum. In a diabetes mellitus-associated erectile dysfunction rat model, the fraction also improved corpus cavernosum architecture, increased smooth muscle content, and reduced fibrosis, although the increase in the intracavernous pressure/mean arterial pressure ratio did not reach statistical significance. Overall, the results suggest the preclinical anti-erectile dysfunction potential of petroleum ether fraction and support its further mechanistic, pharmacokinetic, safety, and translational evaluation.
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高影响因子
Nanoscale Research Letters
·
2019
·Vol. 14
·期 1
·1-10
·
IF 3.159
Zheng Qingkai,Cheng Wenjing,Zhang Xiaoping,Shao Runxia,Li Zhongdong
In this report, we present a pH-induced reversible assembly system (PIRAS) based on ferritin (Ft) for targeted tumor therapy. It has been developed to easily load and release of the anticancer drug resveratrol (RV), based on its natural pH-sensitivity and unique hollow cavity of Ft. A tumor-specific target peptide Arg-Gly-Asp (RGD) was conjugated onto the surface of RV-loaded Ft (RV@Ft), to form biocompatible nanoparticles (RV@Ft-RGD). The pH-sensitivity of Ft allows it to be denatured into a hollow porous nanosphere under acidic condition and renatured into a sealed hollow nanosphere under neutral condition. Using pH manipulation, RV@Ft-RGD, with a ~ 21 nm diameter, showed a high RV loading ratio of 79.6%. pH-triggered RV release was then measured at a ratio of 50.3% at pH5.0 over 24 h. Under neutral condition, the RV@Ft-RGD showed excellent stability over 20 days. Confocal fluorescence imaging showed that RV@Ft-RGD had a high cell uptake ratio and co-localization with the lysosome, mainly due to the RGD-mediated target effect. Based on the high drug loading, pH-triggered release, and tumor cell targeting effect, RV@Ft-RGD showed great cell-killing ability in vitro and in vivo. The biocompatibility in vitro and in vivo was also demonstrated to be excellent, without systematic toxicity. The design concept of PIRAS based on Ft significantly inhibits tumor growth and simultaneously further broadens the application of Ft in nanomedicine.
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PubMed
高影响因子
MACROMOLECULAR RESEARCH
·
2022
·Vol. 30
·期 4
·238-244
·
IF 2.127
Twizeyimana Etienne,Zhang Shuang,Mukerabigwi Jean Felix,Ge Zhishen
Advances in designing smart hydrogels with the structures and properties similar to the interior of extracellular matrix have aroused the interest of researchers as promising cell support scaffolds in the field of tissue engineering. In this study, a novel hydrogel system is developed from natural polysaccharides that are sodium alginate, gelatin and carboxymethyl chitosan through Schiff base cross-linking mechanism. By mixing these three components, the hydrogel system with tunable strength and gelation time was obtained and abbreviated as oxACG hydrogel. Moreover, the obtained hydrogels are biocompatible and can sustain the growth and proliferation of Hela cells after 24 h incubation. The experimental results show that the hydrogel possesses a relatively short gelation time that is around 3 min, high gel strength and negligible cell cytotoxicity. The oxACG hydrogel composite exhibited desirable properties, and they can be used as high-performance multifunctional injectable hydrogels in cell therapy.
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高影响因子
Nano Research
·
2020
·Vol. 13
·期 3
·745-751
·
IF 8.183
Cheng Xiaoling,Yin Zhibin,Rong Liu,Hang Wei
Insights into the pharmacologic effect on cellular processes and the potential toxicological effects are vital to new drug development and evaluation, yet research on these subjects remains a great challenge due to the lack of information regarding the spatiotemporal distribution of drugs and metabolites within a single cell. Mass spectrometry imaging (MSI) has proven to be a label-free and high-throughput approach for visualizing drug distribution in spatial and temporal domains. However, single-cell drug imaging has been limited so far by detection sensitivity and microscale lateral resolution. Herein, we report near-field laser desorption/laser postionization mass spectrometry (NDPI-MS) for single-cell imaging of two structurally similar drugs, proflavine and ethacridine, and subcellular distributions of proflavine at different drug concentrations were investigated. The NDPI-MS imaging results indicate that proflavine was accumulated in lysosomes, which was verified by laser scanning confocal microscopy (LSCM). Additionally, a distinguished subcellular distribution pattern of ethacridine from proflavine could be visualized, highlighting the complexity of the interaction between the drugs and biological environment even though these two drugs possess similar structures. Taken together, the present results demonstrate the great potential of the integrated single-cell MSI platform for characterizing the drug distribution and its phenotype changes within individual cells, expediting the identification and evaluation of newly developed drugs.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 45
·21206-21218
·
IF 4.682
Song Shuang,Xu Guochao,Yang Na,Shahzad Sohail Anjum,Lv Junying,Shen Xiande,Yu Cong
Wound healing is a complicated process that can be susceptible toward infection caused by bacteria. The surging drug resistance has become one of the biggest challenges to the treatment of these infections in public health. Herein, a new asymmetrical aza-boron-dipyrromethene (ABDP) probe is prepared and encapsulated with cholesterol-modified polyethylene glycol polymer (Chol-PEG 2000 -NH 2 ) to construct a PTT/PDT dual-modality antibacterial agent (the ABDP NPs) and provide a new material for the treatment of MRSA-based infections. The ABDP NPs show remarkable wide UV–Vis absorption at 300–850 nm and superior bactericidal effects against Staphylococcus aureus ( S. aureus ), Escherichia coli ( E. coli ), and Methicillin-resistant S. aureus (MRSA), which can be controlled by a phototheranostic antibacterial switch (808 nm laser). Histological analysis indicates that wounds treated by the ABDP NPs plus NIR laser irradiation exhibit more complete re-epithelialization, abundant collagen fibers, and higher hair follicle numbers than the vancomycin-treated group. This study provides a promising perspective using aza-BODIPY dye for the treatment of multidrug-resistant bacteria-based infections and clinical applications in the future.
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高影响因子
Frontiers of Chemical Science and Engineering
·
2019
·Vol. 14
·期 4
·546-560
·
IF 2.809
Wang Hanlu,Jibrin Idris,Zeng Xingye
Catalysts for the desulfurization of gasoline samples were synthesized via the immobilization of well-dispersed phosphotungstic acid (HPW) on Mobil composition of matter-twenty-two (MWW) zeolite. Characterization results indicated that these catalysts possess a mesoporous structure with the retention of the Keggin structure of immobilized HPW. Relevant reaction parameters influencing sulfur removal were systematically investigated, including HPW loading, catalyst dosage, temperature, initial S-concentration, molar ratio of oxidant to sulfide (O/S), volume ratio of MeCN to model oil (Ext./oil), and sulfide species. The 40 wt-% HPW/MWW catalyst exhibited the highest catalytic activity with 99.6% dibenzothiophene sulfur removal from prepared samples. The 40 wt-% HPW/MWW catalyst was recycled four times and could be easily regenerated. Finally, as an exploratory study, straight-run-gasoline and fluid catalytic cracking gasoline were employed to accurately evaluate the desulfurization performance of 40 wt-% HPW/MWW. Our research provides new insights into the development and application of catalysts for desulfurization of gasoline.
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高影响因子
Journal of Pharmaceutical Innovation
·
2021
·Vol. 17
·期 4
·1109-1115
·
IF 2.75
Wang Wenhua,Yang Beibei,Huang Zhengwei,Huang Ying,Hu Ping,Pan Xin,Wu Chuanbin
Solid lipid nanoparticles (SLN) have been intensively exploited as nanocarriers for pulmonary drug delivery. However, no consensus on the underlying mechanisms between particle sizes and cell uptake behaviors was attained owing to their unclear in vivo fate after inhalation, which hindered the further development of a novel nanoparticle delivery system. Recently, aggregation-caused quenching (ACQ) probes had been developed to monitor the integrity of nanocarriers. In this study, an ACQ probe, P4, was employed to clarify the effect of particle size on SLN cellular uptake. SLN (120, 240, and 480 nm in size), incubated with epithelium cells (A549) and macrophages (RAW 264.7), mainly localized in the cytoplasm in an integrated pattern, showing a positive correlation relationship between particle size and cellular intake. In conclusion, this study established a firm basis to understand the effect of particle size on cellular uptake of nanoparticle systems and promoted the development of novel pulmonary drug delivery systems.
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高影响因子
危险品化学品经营许可证（带存储）