CHINESE JOURNAL OF POLYMER SCIENCE
·
2015
·Vol. 33
·期 5
·792-796
·
IF 1.835
Liu Bo,Han Bao-yu,Zhang Chun-ling,Li Shi-hui,Sun Guang-ping,Cui Dong-mei
β -imidophosphonamido ligated lutetium alkyl complex (NPN Dipp )Lu(CH 2 SiMe 3 ) 2 (THF) (NPN Dipp = Ph 2 P(NC 6 H 3
i Pr 2 -2,6) 2 ) with the activation of Al i Bu 3 and [Ph 3 C][B(C 6 F 5 ) 4 ] exhibited high catalytic activity, medium syndio-(rr = 66%) but remarkably high 3,4-regioselectivity for the polymerization of β -myrcene (MY). In sharp contrast, high isotactic 3,4-polymyrcene (PMY) (mm = 95%) was obtained by the precursor (NPN Et )Lu(CH 2 SiMe 3 ) 2 (THF) (NPN Et = PPh 2 (NC 6 H 3
i Pr 2 -2,6)(NC 6 H 4 -Et-2)) with less bulky substituents on the N -aryl ring.
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高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2015
·Vol. 92
·期 4
·495-502
·
IF 1.541
Fu Xiao-bo,Chen Jie,Song Xue-li,Zhang Yuan-ming,Zhu Yi,Yang Jun,Zhang Cheng-wu
A novel carbon solid acid catalyst was prepared by incomplete hydrothermal carbonization of β-cyclodextrin into small polycyclic aromatic carbon sheets, followed by the introduction of –SO 3 H groups via sulfonation with sulfuric acid. The physical and chemical properties of the catalyst were characterized in detail. The catalyst simultaneously catalyzed esterification and transesterification reactions to produce biodiesel from high free fatty acid (FFA) containing oils (55.2 %). For the as-prepared catalyst, 90.82 % of the oleic acid was esterified after 8 h, while the total transesterification yield of high FFA containing oils reached 79.98 % after 12 h. By contrast, the obtained catalyst showed comparable activity to biomass (such as sugar, starch, etc.)-based carbon solid acid catalyst while Amberlyst-15 resulted in significantly lower levels of conversion, demonstrating its relatively high catalytic activity for simultaneous esterification and transesterification. Moreover, as the catalyst can be regenerated, it has the potential for use in biodiesel production from oils with a high FFA content.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2015
·Vol. 33
·期 12
·1721-1729
·
IF 1.835
Yin Xiu-nan,Wang Jing,Zhou Jian-jun,Li Lin
In this manuscript, an easy method of anchoring Au nanoparticles onto a polypropylene (PP) membrane to prepare a composite Au-PP membrane with catalytic activity was demonstrated. The surface of the PP membrane was first modified with a primary amine by mussel-inspired dopamine polymerization. Then, the modified PP membrane was used to reduce chloroauric acid to anchor Au nanoparticles onto the surface, forming a Au-PP membrane. The surface morphology and composition of the modified PP membrane were characterized with SEM, ATR-FTIR and XPS. The catalytic activity of the Au-PP membrane was also evaluated by the degradation of a model dye solution of methylene blue. The fabricated membrane shows excellent catalytic performance, and the catalytic activity can be effectively regenerated.
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高影响因子
WATER AIR AND SOIL POLLUTION
·
2015
·Vol. 226
·期 11
·1-12
·
IF 1.554
Ahmad Ayyaz,Gu Xiaogang,Li Li,Lu Shuguang,Xu Yisheng,Guo Xuhong
In this study, nanosized zero-valent iron-reduced graphene oxide (nZVI-rGO)-activated persulfate (PS) was used to investigate the generation of reactive oxygen species (ROS) for the degradation of trichloroethylene (TCE) in the aqueous solution. More than 98 % of TCE was degraded within 2 min under experimental conditions. The generation of ·OH increased when the pH was shifted toward the basic region while ·SO 4
− radicals’ intensity increased in the acidic pH. Different scenarios have been observed in ·O 2
− generation in the neutral and strong basic pH and decreased in acidic or slightly basic pH. In addition, the intensity of ·OH was increased with the addition of HCO 3
− (10 mM) and NO 3
− (100 mM) but decreased in the presence of Cl − (10 and 100 mM), HCO 3
− (100 mM), and NO 3
− (10 mM). The degradation of anisole, probe for both ·OH and ·SO 4
− , was slightly enhanced by 10 mM NO 3
− anions but decreased in 100 mM salt solution. ·O 2
− intensity was increased while HCO 3
− (10 and 100 mM) and NO 3
− (100 mM) anions were used. nZVI-rGO-activated PS process could remove TCE in aqueous effectively, and the ROS generation and intensity were influenced by solution pH values and anions.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2015
·Vol. 99
·期 21
·9135-9146
·
IF 3.337
Xie Xing,Pang Maoda,Liang Shan,Yu Lei,Zhao Yanbing,Ma Ke,Kalhoro Dildar Hussain,Lu Chengping,Liu Yongjie
Dogs are susceptible to infectious diseases that occur primarily in the respiratory tract. The airway epithelium acts as a first line of defense and is constantly exposed to microorganisms present in the environment. Respiratory epithelial cells have recently gained wide use as a cell model for studying the pathogenesis of human, murine or swine respiratory pathogen infections. However, studies of the pathogenic mechanisms of canine pathogens have been hindered by the lack of reliable respiratory cell lines. Here, we cultured primary canine bronchiolar epithelial cells (CBECs), whose characteristics were confirmed by their expression of the epithelial cell-specific marker cytokeratin 18, and have provided protocols for their isolation and ex vivo expansion. Further, we established immortalized CBECs containing the human telomerase reverse transcriptase (hTERT) gene via transfection of primary CBECs with the recombinant plasmid pEGFP-hTERT. Immortalized bronchiolar epithelial cells (hTERT-CBECs) retain the morphological and functional features of primary CBECs, as indicated by reverse transcriptase polymerase chain reaction, proliferation assays, karyotype analysis, telomerase activity assay, and Western blotting, which demonstrate that hTERT-CBECs have higher telomerase activity, an extended proliferative lifespan, and a diploid complement of chromosomes, even after Passage 50. Moreover, this cell line is not transformed, as evaluated using soft agar assays and tumorigenicity analysis in nude mice, and can therefore be safely used in future studies. The isolation and establishment of stable hTERT-CBECs is of great importance for use as an in vitro model for mechanistic studies of canine pathogenic infections.
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PubMed
高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2015
·Vol. 42
·期 6
·5639-5651
·
IF 1.221
Zhao Zhefei,Ping Niu,Di Jing,Zheng Huajun
The strong selective adsorption property of monoclinic tungsten trioxide nanowires (WO 3 NWs) towards organic dyes was reported in this paper. The effects of pH, initial concentration and types of dyes were systematically investigated. Particularly, methylene blue (MB) was more inclined to be adsorbed in acid solution and a maximum uptake capacity of 148.6 mg g −1 was achieved. 88 % of MB can be rapidly adsorbed within 70 min. The kinetics, isotherms and thermodynamics for the adsorption of MB and methyl orange (MO) were well described. The kinetic adsorption on WO 3 NWs followed a pseudo second-order model ( R
2 = 0.998) and the Langmuir isotherm ( R
2 = 0.992) agreed very well with the experimental data. The negative values of Δ G
0 at various temperatures (−5.656, −5.792, and −5.946 kJ mol −1 ) and Δ H
0 (−1.343 kJ mol −1 ) implied that the adsorption reaction was spontaneous and exothermic. Specific surface area (864.153 m 2 g −1 ) and surface acidic groups of WO 3 NWs enabled excellent adsorption performance. A highly selective adsorption mechanism involving the electrostatic interaction between hydroxyls on the WO 3 NWs' surfaces and different dye molecules was proposed.
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高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2015
·Vol. 124
·期 2
·899-907
·
IF 2.042
Li Xiangyu,Huang Chuan,Yang Hongtao,Li Yanchun,Cheng Yi
The thermal reaction properties of the aluminum nanoparticles/copper (II) oxide nanoparticles/poly(vinylidene fluoride) (Al-NPs/CuO-NPs/PVDF) nanocomposite (mass ratio of Al-NPs/CuO-NPs/PVDF = 20/60/20) were investigated by means of thermogravimetry–differential scanning calorimetry–mass spectrometry (TG/DSC-MS) and X-ray diffraction (XRD) analysis. Al-NPs/PVDF (mass ratio of Al-NPs/PVDF = 50/50) and CuO-NPs/PVDF (mass ratio of CuO-NPs/PVDF = 75/25) nanocomposites were also prepared as a comparison. It is observed that PVDF in the Al-NPs/PVDF nanocomposite acts as an oxidizer. The fluorine ions/fluorinated gases formed at elevated temperatures react with the Al 2 O 3 shell leading to the formation of aluminum fluoride (AlF 3 ). The oxide shell is degraded from the reaction between fluoride ions and Al 2 O 3 , leaving the core Al available for reaction with fluorine ions/fluorinated gases. H
+ 2
( m/z = 2), OH + ( m/z = 17), H 2 O + ( m/z = 18), HF + ( m/z = 20), CF + ( m/z = 31) and CH 2 F + ( m/z = 33) fragments were detected in the gaseous products. For CuO-NPs/PVDF nanocomposite, oxygen was released due to the decomposition of CuO to Cu 2 O and then Cu metal, oxidizing hydrocarbon groups in PVDF. Species of OH + ( m/z = 17), H 2 O + ( m/z = 18), CO
+ 2
( m/z = 44), C 2 H 2 F + ( m/z = 45) and HF + ( m/z = 20) were detected in the gaseous products. The final reduction product of CuO is Cu metal. It is observed that PVDF in the Al-NPs/CuO-NPs/PVDF nanocomposite acts as both oxidizer and reducer in the thermal decomposition. Below 550 °C, PVDF is oxidized by CuO-NPs and oxidizing Al-NPs at the same time, resulting mass reduction. After 550 °C, the remaining Al-NPs and copper (I)/copper (II) oxide will proceed the exothermic solid-state thermite reaction. OH + ( m/z = 17), H 2 O + ( m/z = 18), CO
+ 2
( m/z = 44), C 2 H 2 F + ( m/z = 45) and HF + ( m/z = 20) were the main products of the decomposition of Al-NPs/CuO-NPs/PVDF nanocomposite.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2015
·Vol. 408
·期 4
·1015-1022
·
IF 3.436
Wang Shasha,Chen Zhaopeng,Choo Jaebum,Chen Lingxin
A naked-eye sensitive ELISA-like assay was developed based on gold-enhanced peroxidase-like activity of gold nanoparticles (AuNPs). Using human IgG (H-IgG) as an analytical model, goat anti-human IgG antibody (anti-IgG) adsorbed on microtiter plate and AuNPs-labeled anti-IgG acted as capture antibody and detection antibody, respectively. Because the surfaces of AuNPs were blocked by protein molecules, the peroxidase-like activity of AuNPs was almost inhibited, evaluated by the catalytic oxidation of peroxidase enzyme substrate 3,3′,5,5′-tetramethylbenzidine (TMB), which could produce a bright blue color in the presence of H 2 O 2 . Fortunately, the catalytic ability of AuNPs was dramatically increased by the deposition of gold due to the formation of a new gold shell on immunogold. Under optimal reaction conditions, the colorimetric immunoassay presented a good linear relationship in the range of 0.7–100 ng/mL and the limit of detection (LOD) of 0.3 ng/mL calculated by 3σ/S for UV-vis detection, and obtained LOD of 5 ng/mL for naked-eye detection. The obtained results were competitive with conventional sandwich ELISA with the LOD of 1.6 ng/mL. Furthermore, this developed colorimetric immunoassay was successfully applied to diluted human serum and fetal bovine serum samples, and predicted a broad prospect for the use of peroxidase-like activity involving nanomaterials in bioassay and diagnostics.
A naked-eye ELISA-like immunoassay for determination of human IgG is described based on gold enhanced peroxidase like activity of gold nanoparticles and it is a colorimetric typical sandwich-type immunoassay format with high sensitivity
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高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 183
·期 2
·683-688
·
IF 3.741
Jia Shengsong,Li Pengfei,Koh Kwangnak,Chen Hongxia
The article describes the use of a designed histidine (His)-tagged arginine-glycine-aspartic acid (RGD) peptide as a linker to bind integrin to captured cancer cells, and how to amplify the surface plasmon resonance (SPR) signal after binding of NiO nanoparticles (NiO-NPs) via the His-tag on the peptide. Specifically, breast cancer cells were captured via the interaction between human mucin (MUC-1) and a gold surface modified with a MUC-selective aptamer. The resulting cytosensor exhibits specificity and high sensitivity which is due to the enhancement of the SPR signal by NiO-NPs. The breast cancer cell line MCF-7 can be easily distinguished from normal islet beta cells by using this biosensor. Implementation of the His-tagged RGD peptide modified with NiO-NPs resulted in 20-fold enhancement of the SPR signal at the limit of detection. Hence, the actual limit of detection is lowered to 136 cells per mL. In our perception, this cytosensor has a large clinical potential in that it may also be used to detect various other kinds of tumor cells.
Histidine-tagged arginine-glycine-aspartic acid peptide carried by NiO interacts with the integrin of cancer cells and amplifies the SPR signal resulting from interaction between human mucin (MUC-1) and a gold surface modified with a MUC-selective aptamer. As little as 136 cells·mL −1 are detectable.
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MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 1
·201-208
·
IF 3.719
Luo Jinmei,Zhang Jiahui,Lin Jianxing,Wang Jinhui,Yang Peihui
We report on a simple and rapid method for the enrichment of polyphenolic compounds (pPhCs) by means of gold nanorods whose surface was functionalized with a monolayer of β-cyclodextrin (β-CD-AuNRs) via thiol chemistry. Enrichment is based on the formation of inclusion complexes between pPhCs and β-cyclodextrin through hydrophobic interactions and hydrogen bonding. Fourier transform infrared spectroscopy, mass spectrometry, and transmission electron microscopy were applied to confirm the inclusion of the pPhCs. Moreover, binding leads to a quenching of the red fluorescence of the AuNRs. This effect can be applied to quantify the polyphenols mangiferin, chrysin, and daphnetin with detection limits at 5 nM, 15 nM, and 20 nM concentrations, respectively. Water samples were spiked with pPhCs, and their extraction by using β-CD-AuNRs gave recoveries ranging from 97.6 to 110.2 %.
Gold nanorods were functionalized with β-cyclodextrin via thiol chemistry. Based on the formation of inclusion complexes between polyphenolic compounds (pPhCs) and β-cyclodextrin through hydrophobic interactions and hydrogen bonding, β-cyclodextrin functionalized gold nanorods as fluorescent probe to simple, rapid and quantitative detect polyphenolic compounds
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Food Analytical Methods
·
2014
·Vol. 8
·期 2
·428-437
·
IF 1.802
Zeng Fang,Ou Jun,Huang Yufen,Li Qian,Xu Guizhi,Liu Zhongzhen,Yang Shaohai
The present paper reports the application of an optimized pre-column derivatization procedure with aminoquinolyl- N -hydroxysuccinimidyl carbamate (AQC) to the determination of 21 free amino acids in fruit juices. The method permitted the optimal separation of 21 amino acids and ammonium in 30 min. Excellent response linearity was obtained from 2 to 1,000 μM for all amino acids, except tryptophan, for which the linearity was 5–1,000 μM. The fluorescence detection limits ranged from 0.08 pmol (phenylalanine) to 0.69 pmol (cysteine), except for tryptophan (7.89 pmol), for a signal-to-noise ratio of 3. The relative standard deviations of peak areas ranged from 0.1 to 0.4 % for intraday analysis and from 2.1 to 5.0 % for interday analysis. The method was applied to analyze the free amino acids in six fruit juices, and the recoveries of the proposed method were 90.4 to 103.3 %. The total free amino acid content ranged from 56.97 mg L −1 (apple) to 469.45 mg L −1 (longan) in six fruit juices. Aspartic acid (Asp), glutamic acid (Glu), asparagine (Asn), serine (Ser), and glutamine (Gln) were present in higher levels in most samples, whereas cysteine (Cys) was below the detection limit in the selected samples. Notable advantages of this method include its speed, ease, low cost, good repeatability, high sensitivity, excellent precision and accuracy, and anti-interference in high-sugar matrices.
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Journal of Central South University
·
2014
·Vol. 21
·期 7
·2645-2651
·
IF 0.464
Zhao Jin-yao,Li Li,Jiao Fei-peng,Ren Feng-lian
The human plasma protein binding of water soluble flavonoids in the peels of five spices of citrus fruits was studied by ultrafiltration combined with HPLC. The flavonoids were extracted separately by hot and cold water, and higher total flavonoid contents were detected in the former extracts than the latter ones. All the extracts show significant scavenging abilities to both ABTS and DPPH free radicals, which indicates the health benefits of the water extracts of citrus fruits peels. For DPPH radical, the IC 50 values of hot extract follow as Navel orange (NO)≈Mandarin orange (MO)< Lemon (LE)< Lo tangerine (LO)< Pomelo (PO), while the rank is NO< PO
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危险品化学品经营许可证（带存储）