GREEN CHEMISTRY
·
2017
·Vol. 19
·期 20
·4838-4848
·
IF 9.125
Gang Wang,Yiming Xu,Suojiang Zhang,Zengxi Li,Chunshan Li
A one-pot synthesis of α,β-unsaturated esters from unactivated esters and aldehydes using strong bases, such as sodium alkoxide and potassium tert-butoxide, was reported. However, the ionic liquid (IL) catalyzed probase method for producing α,β-unsaturated esters was not reported until now. In this work, a series of ILs with fluoride anions were firstly prepared and used as catalysts in combination with the probase N,O-bis(trimethylsilyl) acetamide (BSA) for the α,β-unsaturated esters synthesis. This process could also be promoted through the introduction of another IL with Lewis acid sites. The yield and selectivity of the product could reach up to 84.2% and 95.0%, respectively, when [Bmim]F was used in combination with [Bmim]Cl/AlCl3 (the molar fraction of AlCl3 is 0.67). The mechanism investigation through GC-MS indicates that BSA would convert into onium amide, which acted as a strong base for α-H abstraction, with the catalysis of [Bmim]F. Meanwhile, [Bmim]Cl/AlCl3 played an important role in the condensation step between enolates and aldehydes. On the basis of mechanism insights, kinetic and thermodynamic studies were also carried out for a better understanding of this new route.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Runqiang Liu,Bo Li,Fang Li,Volodymyr Dubovyk,Yuqi Chang,Dongdong Li,Kunjie Ding,Qiwen Ran,Guifang Wang,Hongyuan Zhao
Carbendazim (CBZ) abuse always causes the over-standard of pesticide residues in agricultural products, which has adverse effects on human health. Herein, a novel electrochemical sensor was firstly fabricated based on the β-cyclodextrin (β-CD) functionalized carbon nanosheets@carbon nanotubes (CNS@CNT) for the CBZ determination. CNS@CNT combined large surface area of CNS and excellent electrical conductivity of CNT, which significantly enhanced the electrocatalytic performance. Moreover, β-CD possessed excellent host-gest supramolecular recognition ability, which could improve the selective recognition and enrichment capability of CBZ. Thanks to the synergistic interaction of CNS@CNT and β-CD, the β-CD/CNS@CNT/GCE sensor exhibited a low limit of detection of 9.4 nM in the linear CBZ concentration range of 0.03–30 μM. The fabricated sensor presented favorable stability, high sensitivity (30.86 μA μM −1 cm −2 ), and reliable reproducibility (RSD = 3.6%). Especially, the β-CD/CNS@CNT/GCE sensor could show pretty practical feasibility for the detection of CBZ in apple juice with recoveries of 97.1%-99.4%.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Xiya Zhang,Fenfen Zhao,Yawei Sun,Tiejun Mi,Liye Wang,Qian Li,Jiayin Li,Wentao Ma,Wanjing Liu,Jingnan Zuo,Xinyu Chu,Bing Chen,Wenxin Han,Yexuan Mao
This study aimed to improve the sensitivity of lateral flow immunoassay (LFIA) using two strategies, including using a high sensitivity label and an indirect-labeling format, which were simultaneously applied to develop a highly sensitive LFIA to detect 22 β-lactams. In the new format, β-lactam receptors were used without any labeling, and anti-receptor monoclonal antibodies (mAbs) were labeled with amorphous carbon nanoparticles (ACNs). The new LFIA is based on the following steps: 1) screening mAbs against the receptors for antigen-antibody binding sites away from the active site of the receptors; and 2) detecting immune complexes using receptor-mAb-ACNs. The cut-off values against ampicillin, amoxicillin, benzylpenicillin, dicloxacillin, cloxacillin, oxacillin, nafcillin, cefquinome, cefoperazone, cefapirin, ceftazidime, cephalothin, cefepime, cefoxitin, cefazolin, ceftriaxone, ceftiofur, cefotaxime, cefalexin, imipenem, meropenem, and ertapenem were 1, 2, 2, 10, 10, 10, 10, 3, 3, 3, 5, 10, 10, 20, 20, 20, 30, 30, 100, 10, 15, and 15 ng/mL, respectively, which is significantly lower than that of receptor-mAb-colloidal gold-LFIA using the same immune reagents.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Adeel Ahmed,Muhammad Usman,Bing Yu,Fengyuan Gao,Youqing Shen,Hailin Cong
The unintended leakage of endocrine-disrupting bisphenol A (BPA) from different industries into the aquatic environment has posed a severe threat to aquatic lives and human health. Herein, we explored a hydrothermal method to synthesize superparamagnetic β-cyclodextrin-cobalt ferrite (β-CD-CoFe 2 O 4 ) catalyst for the selective capturing and efficient degradation of BPA through heterogeneous activation of peroxymonosulfate (PMS). The as-prepared catalysts were systematically characterized through multi-techniques to explore the relationship between their catalytic performance and material properties. Results showed that, in β-CD-CoFe 2 O 4 /PMS system, the degradation rate of BPA reached 99.23% in 60 min with a rate constant of 0.0708 min −1 , approximately 16.09 times than CoFe 2 O 4 /PMS system (0.0044 min −1 ). Specifically, the large surface area and availability of more reactive sites of β-CD-CoFe 2 O 4 boosted the activation process of PMS compared to pristine CoFe 2 O 4 , thus enhancing the degradation of BPA. Meanwhile, the effect of different operational parameters and co-existing anions on BPA degradation were also investigated. The generation of free radicals ( S O 4 ∙ - , ∙ O H , O 2 ∙ - , a n d 1 O 2 ) in the β-CD-CoFe 2 O 4 /PMS system liable for reducing BPA was corroborated through electron paramagnetic resonance analysis and quenching experiments. The possible degradation pathways of BPA were proposed based on the identification of reaction intermediates formed during the mineralization process. In brief, the findings of this study suggested that β-CD-CoFe 2 O 4 is an efficient and environmentally friendly catalyst that facilitated the degradation of BPA through PMS activation. Moreover, the excellent magnetic separation, stability, and reusability of the β-CD-CoFe 2 O 4 catalyst make them promising candidates for their water purification applications.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Huimin Duan,Xiaojiao Wang,Yanhui Wang,Yuanling Sun,Jianbo Li,Chuannan Luo
In this work, ionic liquid modified Fe 3 O 4 @dopamine/graphene oxide/ β -cyclodextrin (ILs-Fe 3 O 4 @DA/GO/ β -CD) was used as supporting material to synthesize surface molecularly imprinted polymer (SMIP) which then was introduced into chemiluminescence (CL) to achieve an ultrasensitive and selective biosensor for determination of lysozyme (Lys). ILs and β -CD was applied to provide multiple binding sites to prepare Lys SMIP and Fe 3 O 4 @DA was designed to make the product separate easily and prevent the aggregation of GO which could improve absorption capacity for its large specific surface area . The ILs-Fe 3 O 4 @DA/GO/ β -CD-SMIP showed high adsorption capacity ( Q = 101 mg/g) to Lys in the adsorption isotherm assays. The adsorption equilibrium was reached within 10 min for all the concentrations, attributing to the binding sites situated exclusively at the surface, and the adsorption model followed Langmuir isotherm. Under the suitable CL conditions, the proposed biosensor could response Lys linearly in the range of 1.0 × 10 −9 –8.0 × 10 −8 mg/mL with a detection limit of 3.0 × 10 −10 mg/mL. When used in practical samples in determination of Lys, the efficient biosensor exhibited excellent result with the recoveries ranging from 94% to 112%.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Jinshui Liu,Guoning Liu,Wenxiu Liu
Novel nanocomposites of β-cyclodextrin/poly(acrylic acid) grafted onto graphene oxide (β-CD/PAA/GO) were successfully synthesized for the first time using an esterification reaction, and they were used to remove the cationic dyes methylene blue (MB) and safranine T (ST) from aqueous solutions. The as-prepared β-CD/PAA/GO nanocomposites were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The β-CD/PAA/GO nanocomposites showed excellent water dispersibility because of the hydrophilicity of the polymer. The adsorption isotherms fitted the Langmuir isotherm model. The maximum adsorption capacities were 247.99 mg g −1 for MB and 175.49 mg g −1 for ST, and these are much higher than those of other carbon-based adsorbents that have been described in the literature. This was attributed to the high concentration of functional groups in the nanocomposites. Repeated MB and ST adsorption and desorption cycles showed that the β-CD/PAA/GO nanocomposites could be used repeatedly, and their adsorption capacities did not change significantly after five cycles. The fast and efficient adsorption of MB and ST by the β-CD/PAA/GO nanocomposites from aqueous solutions suggested that these novel nanocomposites may be ideal candidates for removing dyes during water treatment.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Ying Zhang,Wang Ren,Yu Zhu Fan,Hong Qun Luo,Nian Bing Li
Monitoring the concentration of norepinephrine (NE), epinephrine (Ep) and dopamine-β-hydroxylase (DβH) is of great importance in studying and diagnosing related diseases. In this study, a simple chemically-modulated turn-on fluorescence strategy is proposed for rapid, selective and visual discrimination of trace NE and Ep. Herein, Ep is differentiated from other catecholamines via its fast fluorescence response in 1.0 M NaOH medium. Furthermore, NE is alkalized with 0.01 M NaOH and is then immediately triggered by polyethyleneimine to emit bright cyan fluorescence, whereas Ep and DA show no any fluorescence response. The fluorescence intensity of resultant solution is linearly proportional to the concentration of Ep and NE in the range of 50.0–10000.0 nM and 10.0–10000.0 nM, respectively. Moreover, NE and Ep as low as 500.0 nM can be fluorescently identified with naked-eye, which is convenient for rapidly preliminary diagnosis and self-test of hypercatecholaminism. Inspired by the catalytic property of DβH transforming DA into NE, the proposed strategy enables a simple and high-efficient fluorescence method to monitor DβH by using trace DA as substrate and directly quantifying NE. The method is simple, smart, highly efficient and eco-friendly, and has been applied to the rapid assay of NE, Ep and DβH in real samples.
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高影响因子
ARCHIV DER PHARMAZIE
·
2025
·Vol. 358
·期 3
·e2400796
·
IF 3.6
Yating Guo,Meng Cao,Zhaohui Li,Hongfei Zhou,Zhuo Chen,Qianbin Li
Hyperactivation of the Wnt/β-catenin signaling pathway has been widely recognized as a pathogenic mechanism for colorectal cancer (CRC). Based on a previously reported lead compound, iCRT14, a series of 2,4-thiazolidinedione derivatives were designed, synthesized, and evaluated in vitro for their antiproliferative activity against colon cancer cells. Compound 15k exhibited the most potent activity against the HCT116 and SW480 cell lines. Compound 15k inhibited Wnt/β-catenin signaling by disrupting the protein–protein interactions between β-catenin and TCF4. Compound 15k simultaneously inhibited tubulin polymerization, disorganized the microtubule network, and arrested the cell cycle at the G2/M phase, offering an additional mechanism of action and 15k induced cell apoptosis by activating caspase-3 and poly(ADP-ribose) polymerase. Additionally, compound 15k inhibited cell migration without affecting the levels of β-catenin protein. These results offer guidance for developing the current series as potential new anticancer therapeutics.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Guoquan Zhang,Shaowei Hu,Xiaoyu Zhu,Xiaoxin Hu,Fenglin Yang
An interlocking “ T -shape” structure was built by uniform filling β-PbO 2 crystalline inside the trace Ti 3+ self-doping TiO 2 nanotube arrays (TNAs) channels and depositing the cauliflower-like β-PbO 2 clusters on its upper surface through a facile current pulse deposition method. We elaborated the interfacial engineering strategy for generating compact oxide layers at TNAs bottoms and growing β-PbO 2 crystalline from the selectively conductive TNAs bottoms. Different characterization techniques and electrochemical analyses were conducted to characterize the morphology, structure, and electrochemical property of the β-PbO 2 partial filling/covering TNAs (β-PbO 2 /TNAs/Ti) materials. We also systematically investigated and elucidated the performance and mechanism of β-PbO 2 /TNAs/Ti anode activated peroxymonosulfate (PMS) system via berberine removal/mineralization, reactive oxygen species (ROS) identification/contribution, intermediates analysis, energy consumption and practical applicability estimation. Results illustrated that filling β-PbO 2 inside TNAs can efficiently improve the directional electron-transfer efficiency, oxygen evolution over-potential, tolerance to the varied water matrices and service lifetime of β-PbO 2 /TNAs/Ti anode activated PMS system. The ROS including • OH, S O 4 ∙ - and 1 O 2 that generated from PMS electro-activation are responsible for efficient berberine degradation through two pathways. This study will provide a general approach to construct the versatile tube-filled TNAs-based materials for wide applications in water/wastewater treatment.
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高影响因子
Food Bioscience
·
2024
·
IF 4.8
Fuhao Tang,Baoyao Wei,Chao Qin,Li Huang,Ning Xia,Jianwen Teng
Passion fruit peel contained various phenolic compounds with α-glucosidase and α-amylase inhibitory activities in vitro . However, the bound state and glycosylated forms hindered their active expression. In this article, β-glucosidase-producing Lactobacilli were inoculated into fermented peels for targeted metabolism. The results showed that the release of bound polyphenols and the increase of free polyphenols were significantly correlated with β-glucosidase activity. The strain G1 with high enzyme activity degraded 48.20% of the bound polyphenols after 24 h. The ability of extracts to inhibit α-glucosidase and α-amylase activities was significantly enhanced and closely related to the content of free polyphenols (r = -0.96 and -0.88, P < 0.001). Computer- assisted screening identified 38 phenolic compounds as potential dual inhibitors of α-glucosidase and α-amylase. Among them, 22 α-glucosidase inhibitors and 24 α-amylase inhibitors were identified for the first time. Various inhibitors, including phenolic acids, flavones, and flavonols, increased in content after fermentation. The total content of 14 glycosylated polyphenols decreased to 28.38% at 48 h, which was opposite to the aglycone. Molecular docking indicated that glycosylated polyphenols bind to β-glucosidase through hydrogen bonds and van der Waals forces. Different glycosylations at the C-3 position of quercetin bound at same sites to β-glucosidase. This study contributes to the theory of enhancing the functional activity of natural products through targeted fermentation transformation.
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Journal of Translational Medicine
·
2025
·Vol. 23
·期 1
·1-18
·
IF 7.5
Dong Rui,Li Meihui,Gu Xiu Feng,Gao Haiting,Wei Ziyi,Qi Houbao,Zhang Jun,Feng Qiang
Background Periodontitis is a chronic inflammatory disease that significantly impacts periodontal bone regeneration, yet the distinct biological features of osteoblasts in this condition remain poorly understood. This study aims to elucidate the cellular and molecular mechanisms underlying osteoblast dysfunction in periodontitis, with a focus on the role of Fusobacterium nucleatum (Fn) and its effector protein, D-galactose-binding periplasmic protein (Gbp).MethodSingle-cell RNA sequencing (scRNA-seq) data from human gingival tissues of periodontitis patients (PD) and healthy controls (HC) were analyzed to identify cellular heterogeneity and molecular pathways. An experimental periodontitis model in mice and primary osteoblast cultures were used to investigate the effects of Fn and Gbp on osteogenic differentiation. Transcriptomic analysis, gene set enrichment analysis (GSEA), and protein-protein interaction (PPI) networks were employed to explore the underlying mechanisms.Results scRNA-seq revealed a reduction in mesenchymal stem cells (MSCs) and osteoblastic lineage cells in PD tissues, with significant downregulation of osteogenic pathways such as Wnt signaling. Fn infection induced alveolar bone destruction in vivo and inhibited osteoblast proliferation, differentiation, and mineralization in vitro. Gbp, an Fn adhesin, similarly impaired osteogenic differentiation by downregulating key osteogenic genes and pathways. Transcriptomic analysis identified shared inflammatory and osteogenic pathways affected by Fn and Gbp, with NF-κB signaling activated and Wnt/β-catenin signaling inhibited. Mechanistically, Gbp interacted with the host protein ANXA2, disrupting the ANXA2/GSK3β complex and inhibiting Wnt/β-catenin signaling, a pivotal route for osteoblast differentiation. ANXA2 knockdown mitigated the Fn/Gbp-induced suppression of osteogenic activity, emphasizing its role in Fn-induced bone loss.Conclusion This study demonstrates that Fn and its effector Gbp disrupt osteogenic differentiation by inactivating the Wnt/β-catenin pathway binding to ANXA2.
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产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Cheng Zeng,Xiangqing Ji,Yu Shi,Siyu Mu,Yicheng Huang,Meiqiao Zhong,Yan Han,Cuicui Duan,Xiaolei Li,Dan Li
To develop a new method for enzymatic preparation of minor ginsenosides , T. stercorarium β-glucosidase (Tsbgl) was characterized and its activities of deglycosylation towards natural ginsenosides were examined. The substrates of 1 mmol l −1 were incubated with the enzyme of 38.3 U ml −1 at 65 ℃ and pH 5.0. The K m values of Tsbgl for ginsenosides Rb 1 , Rg 1 and p NPG were 0.37 ± 0.03, 3.26 ± 0.19, and 1.24 ± 0.03 mmol l −1 , and the V max values were 183.63 ± 7.15, 85.03 ± 4.90, and 117.66 ± 1.96 μmol mg −1 min −1 , respectively. The molar conversion of ginsenosides Rb 1 , Rb 2 , Rb 3 , Rc, Re, Rg 1 , and Rf by Tsbgl within 6 h was 100%, 50.1%, 42.7%, 92.0%, 57.3%, 67.9%, and 76.8%, respectively. The yield of aglycone protopanaxadiol was 35.5 μmol l −1 h −1 for Rb 1 , while the yields of aglycone protopanaxatriol were 64.2 and 70.4 μmol l −1 h −1 for Rg 1 and Rf. Tsbgl with good organic solvent tolerance, mild reaction conditions and broad substrate specificity , could completely remove all outer glucosyls at the C-3 and C-20 hydroxyls of protopanaxadiol-type ginsenosides, and the C-6 and C-20 hydroxyls of protopanaxatriol-type ginsenosides through various pathways, providing a specific and efficient way to produce minor ginsenosides.
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PubMed
高影响因子
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