iScience
·
2023
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IF 5.8
Luxian Guo,Yangming Ding,Hua Wang,Yuxuan Liu,Qian Qiang,Qi Luo,Fei Song,Changzhi Li
The catalytic conversion of lignin into N-containing chemicals is of great significance for the realization of value-added biorefinery concept. In this article, a one-pot strategy was designed for the transformation of lignin β-O-4 model compounds to imidazo[1,2-a]pyridines in yields up to 95% using 2-aminopyridine as a nitrogen source. This transformation involves highly coupled cleavage of C-O bonds, sp 3 C-H bond oxidative activation, and intramolecular dehydrative coupling reaction to construction of N-heterobicyclic ring. With this protocol, a wide range of functionalized imidazo[1,2-a]pyridines sharing the same structure skeleton as those commercial drug molecules, such as Zolimidine, Alpidem, Saripidem, etc., were synthesized from different lignin β-O-4 model compounds and one β-O-4 polymer, emphasizing the application feasibility of lignin derivatives in N-heterobicyclic pharmaceutical synthesis.
研究领域:
有机化学
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生物炼制
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药物化学
✓ 已验证
DOI
高影响因子
POLYMER
·
2021
·
IF 4.43
Hongchao Yan,Qian Jiang,Jing Wang,Shuang Cao,Yuan Qiu,Hong Wang,Yonggui Liao,Xiaolin Xie
It has been attracted broad interest to construct stimulus responsive supramolecular hydrogels as smart materials over the past few decades; however, the design of multi-stimuli responsive hydrogels remains a formidable challenge. Herein, a multi-stimuli responsive supramolecular hydrogel has been designed on the basis of methoxy-azobenzene-grafted poly (acrylic acid) (PAA-mAzo) and β-CD dimer, which involves the host-guest and electrostatic interactions. Besides the common thermo-responsive behavior, the hydrogel also exhibits reversible sol-gel transition under green and blue light irradiations because of the disassociation and association of host–guest interaction between mAzo and β-CD. In addition, the hydrogel turns into precipitate at low pH due to the decrease of solubility of PAA in aqueous solution. The electrostatic interaction between the negatively charged carboxylic acid groups and hydrated protons at high pH makes the recovery of hydrogel. At last, the release of rhodamine B loaded in the triple-stimuli responsive hydrogel can be accelerated by green light irradiation and low pH, which demonstrates the potential applications in biomedical materials .
研究领域:
材料科学
·
生物医药
✓ 已验证
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
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IF 3.3
Wu Dan,Khan Sulaiman,Zhang Shujie,Wang Huan,Chen Wei,Wang Shenqi
Enzymes, key catalysts in biochemical reactions, are prone to denaturation under harsh conditions, leading to reduced stability and higher costs. Enzyme immobilization, using carriers like magnetic nanoparticles, metal–organic frameworks, and viruses, is a common solution. T4 bacteriophage, a virulent E. coli phage containing 155 Hoc and 870 Soc proteins, offers a cost-effective and highly stable platform for enzyme immobilization. In this study, Soc-β-galactosidase (Soc-β-gal) was immobilized on the surface of T4 bacteriophage via affinity fixation and further encapsulated with a metal-polyphenol network (MPN) coating. Comparative analysis of the biochemical properties revealed that the immobilized enzyme, β-gal T4, retained over 85% activity after 6 h at 50 °C, while free Soc-β-gal retained only 40.63%. Moreover, β-gal T4@TA-Ti demonstrated superior stability, retaining 92.88% of its activity after 6 h of UV exposure, compared to 10.21% for β-gal T4 and 7.23% for Soc-β-gal. The MPN coating also enhanced resistance to proteolytic degradation, with β-gal T4@TA-Ti retaining 9.48% of its activity after exposure to proteinase K, in contrast to 4.62% for β-gal T4. Overall, these results demonstrate that enzyme immobilization significantly enhances stability, while the MPN coating further improves resistance to extreme pH, ultraviolet radiation, and other environmental stressors, highlighting the potential of this approach for biocatalytic applications.
研究领域:
生物化学
·
酶工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2025
·
IF 7.9
Qi Lai,Shaorong Huang,Yu Wu,Hu Bei,Shuilin Chen,Kun Zhang,Yuqin Xu,Xiaoyong Zhang,Guicai Sun,Yulong Ouyang
Photothermal therapy has rapidly gained attention as a promising strategy for tumor treatment, owing to its noninvasive nature, ease of operation, high spatial precision, and minimal risk of developing drug resistance. Despite its advantages, the simple and efficient synthesis of photothermal agents (PTAs) that simultaneously exhibit high photothermal conversion efficiency, excellent water dispersibility, low toxicity, biodegradability, and strong therapeutic performance remains a significant challenge. In this study, we successfully synthesized a benzobisthiazole-based conjugated polymer (DPD-CAN-OH) bearing pendant β-cyclodextrin groups via a click condensation reaction. Doxorubicin was subsequently loaded onto DPD-CAN-OH through the formation of a host–guest inclusion complex, resulting in the DPD-CAN-OH-DOX formulation. Characterization results demonstrated that both DPD-CAN-OH and DPD-CAN-OH-DOX exhibit small particle sizes, high water dispersibility, excellent photothermal conversion efficiency, and favorable biodegradability. Biological assays further confirmed that DPD-CAN-OH is associated with low cytotoxicity and good biocompatibility. Notably, DPD-CAN-OH showed significant potential for osteosarcoma treatment by enhancing reactive oxygen species generation and inducing apoptosis in cancer cells. Importantly, the DPD-CAN-OH-DOX complex exhibited markedly superior antitumor efficacy compared to treatment with doxorubicin or DPD-CAN-OH alone, highlighting the therapeutic benefits of combining chemotherapy with photothermal therapy. Considering the straightforward and efficient synthesis process, along with the excellent physicochemical and biological characteristics of the PTAs developed, we believe this work provides valuable new insights into the design and fabrication of multifunctional PTAs for effective cancer therapy.
研究领域:
肿瘤学
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材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
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2022
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IF 11.504
Yinyue Yang,Ming Zhang,Junhua Li,Yujie Su,Luping Gu,Yanjun Yang,Cuihua Chang
This study explored the construction of egg white protein particles (EWPP) and/or rhamnolipid (Rha) based emulsion gels with β-sitosterol (Sit) as gelation factor. The properties of the emulsion gels and the application in cookies were investigated to further reveal the functional mechanism of each component. The rheological and microstructure results of the emulsions showed that emulsion gels had suitable plastic texture with lower melting rate and faster cooling rate compared with butter. EWPP stabilized the droplet while Rha reduced the droplet size and enhanced the fluidity of the emulsion gels. In cookie system, Sit in the emulsion gels provided the appropriate hardness and uniform matrix with emulsifiers. EWPP and Rha effectively improved the appearance, texture and mouthfeel of cookies and extended cookies’ frangibility during storage. The constructed composite emulsion gels showed promising potential as plastic fat substitutes, to be used in healthy bakery systems to decrease saturated fatty acids.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
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IF 3.412
Qianqian Geng,Jingchan Xie,Xin Wang,Mengling Cai,Hui Ma,Hanwen Ni
A water-soluble inclusion complex for butachlor was prepared by complexation with (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD). Phase solubility results indicated a 1:1 stoichiometric ratio with an apparent stability constant of 864.3 M–1 in the obtained solid complex. The formation of the complex was confirmed by 1H nuclear magnetic resonance, Fourier transform infrared, and differential scanning calorimetry spectra. Coupled with the molecular docking results, butachlor was considered to be completely included in HP-β-CD cavity. Butachlor complexation with HP-β-CD decreased its adsorption capacity and enhanced its mobility in soil. The inclusion complex displayed better herbicidal activities than free butachlor. The 96 h median lethal concentration values of the inclusion complex and free butachlor was 2.30 and 0.65 mg L–1, respectively, for zebrafish, indicating that the complexation could significantly reduce toxicity to fishes. The present study provides an approach to develop environment-friendly formulations using CDs for herbicides.
研究领域:
环境科学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
LANGMUIR
·
2022
·
IF 4.331
Yafeng Wang,Xian Luo,Wenjie Lu,Bin Huang,Yanqing Yang
In this study, β-Ni(OH)2 with a unique flower-like morphology was synthesized through a hydrothermal method. The doping of Rh in β-Ni(OH)2 was achieved by a reduction method. The as-synthesized catalysts were characterized by X-ray diffraction, transmission electron microscopy, and X-ray photoelectron spectroscopy for the crystal structure, morphology, composition, and chemical state analysis. The electrochemical tests revealed that the doping of Rh can significantly increase the electrocatalytic performance of β-Ni(OH)2 in 1.0 M KOH solution. The methanol oxidation peak current density of Rh-doped β-Ni(OH)2 reached 95 mA cm–2 with a Tafel slope of 40 mV dec–1. The reason for these improvements is that Rh can suppress the phase transformation of NiOOH from β to α. Meanwhile, the electronic structure change of nickel in β-Ni(OH)2 and the defect ratio increase caused by Rh doping are beneficial to methanol oxidation reaction (MOR) catalytic activity. Furthermore, the synergistic effect between Rh and Ni(OH)2 improved the surface activity of β-NiOOH. The doping of Rh in β-Ni(OH)2 is initiative in this work, which provides a new strategy to design highly efficient and cost-effective MOR electrocatalysts for alkaline DMFCs.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2025
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IF 4.7
Xingfang Zhang,Liang Gao,Jianv Wang,Wei Zhang,Dong Xu,Yanhua Wang,Tianlong Liu,Kai Gao,Zhen Ren,Yi Ding
β-Bisabolene, a bioactive sesquiterpene extracted from myrrh—a substance utilized for ischemic stroke treatment—has known therapeutic potential. Nevertheless, the exact mechanisms underlying its beneficial effects on ischemic stroke, along with its potential targets, remain to be fully elucidated. This study aimed to investigate the therapeutic potential of β-Bisabolene in ameliorating ischemic stroke, identify its potential targets and interactions, and explore the underlying pathways involved. Bioinformatics analyses, including network pharmacology and analysis of GEO transcriptomic datasets, were performed to predict the potential targets and mechanisms of β-Bisabolene in ischemic stroke treatment. Preliminary computational validation was achieved through molecular similarity comparisons, molecular docking studies, and molecular dynamics simulations. In vitro experiments were then conducted to confirm the protective effects of β-Bisabolene on microglia injured by oxygen‒glucose deprivation and to validate the findings from the bioinformatics analyses. The results revealed that β-Bisabolene reduces the levels of the proinflammatory cytokines TNF-α, IL-1β and NO; decreases reactive oxygen species levels; and decreases the expression of COX-2, P38, ERK and Keap1. Moreover, it increases the expression of Arg-1, Nrf2, NQO1 and HO-1. These effects are associated with improved survival rates of oxygen–glucose deprivation–damaged microglia. In conclusion, β-Bisabolene may exert anti-inflammatory and antioxidant effects to ameliorate microglial injury induced by ischemic stroke by antagonizing COX-2 and mediating the MAPK signaling pathway and Keap1/Nrf2 pathway. This study provides valuable insights into the therapeutic potential of β-Bisabolene for the treatment of ischemic stroke.
研究领域:
药理学
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神经科学
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生物信息学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Processes & Impacts
·
2025
·
IF 3.9
Xiaomin Ren,Jianying Wang,Fenqing Zhao,Pingping Zhang,Huan He,Zhixiang Xu,Bin Huang,Xuejun Pan
Previous studies have indicated that 6:2 chlorinated polyfluoroalkyl ether sulfonate (6:2 Cl-PFAES), a substitute for perfluorooctane sulfonate (PFOS), caused disruptions in glucose metabolism, but its toxicological mechanisms remain unclear. Pancreatic β cells are responsible for regulating glucose metabolism. This study used mouse insulinoma β cells (β-TC-6) to study the toxic effects of 6:2 Cl-PFAES and elucidate its potential mechanisms. Through cell counting kit-8, trypan blue staining, and apoptosis assays, we found that 6:2 Cl-PFAES showed toxicity to β-TC-6 cells, even surpassing that of PFOS. Mechanistic studies demonstrated that 6:2 Cl-PFAES induced oxidative stress by ascendant levels of reactive oxygen species (ROS) and malondialdehyde (MDA), alongside increased superoxide dismutase (SOD) activity. The oxidative stress-inducing capability of 6:2 Cl-PFAES was stronger than PFOS. Co-exposure with N-acetyl-L-cysteine (NAC), as the ROS scavenger, which obviously reduced the degree of cell apoptosis and the production of ROS, highlighting oxidative stress as a crucial mechanism of 6:2 Cl-PFAES-induced cell death. Transcriptomic analysis further revealed that 6:2 Cl-PFAES affected genes association with the oxidative phosphorylation pathway, confirming the oxidative stress effect, and altered diabetes-related genes expression, suggesting a heightened risk of diabetes. This study provides insights into the toxicity of 6:2 Cl-PFAES on β cells and its potential risks on glucose metabolism disruption.
研究领域:
毒理学
·
分子生物学
·
环境健康科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
·
2016
·
IF 5.083
Lang-Hong Wang,Man-Sheng Wang,Xin-An Zeng,De-Ming Gong,Yan-Bo Huang
Background Some antibacterial agents exert their antimicrobial action by targeting the cytoplasmic macromolecules , such as proteins or nucleic acids , to disturb the properties of macromolecules that may deeply influence their biological activities and functions. Cinnamaldehyde (CIN) is a natural antibacterial ingredient found in the bark and leaves of cinnamon trees . Methods The inhibitory mechanism of a typical enzyme , β-galactosidase by CIN was investigated by UV–visible, fluorescence, 3-D spectroscopy, circular dichroism , atomic force microscopy and molecular modeling studies. Results CIN decreased the activity of β-galactosidase by competitive inhibition through a multiphase kinetic process. 3-D spectroscopy and circular dichroism showed that the binding of CIN to β-galactosidase resulted in changes in micro-environment of tryptophan and tyrosine residues, and conformation of β-galactosidase. The molecular recognition was also analyzed through modeling which indicated that CIN was inserted into the active site pocket of β-galactosidase and interacted with amino acid residues, such as Met502, Trp568, Phe601 and Trp999. Atomic force microscopy showed that a serious destabilization of the native conformation of β-galactosidase occurred after binding with CIN, e.g., morphological changes and increased dimensions of the β-galactosidase molecule. Moreover, it was found that the combinations of CIN, carvacrol and thymol exposure displayed synergistic effects on the inhibition of β-galactosidase. General significance This study exhibits a comprehensively understanding about the action mechanism of CIN that affects the conformation and activity of β-galactosidase in biochemical processes and provides some new insights into the possible intracellular targeting behaviors of CIN at a molecular level.
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2021
·
IF 3.196
Qianqian Geng,Pingyang Sun,Gang Tang,Pingliang Li,Yuxiu Zhai
Poor water solubility results in limited fungicidal bioavailability and poses a great risk to non-target organisms due to the use of organic solvent in formulation processing. Here, the water solubility of fludioxonil (Flu) was improved by generating an inclusion complex with β-cyclodextrin (β-CD) at 1:1 host-guest stoichiometric ratio. The formation of the inclusion complex was confirmed by SEM, XRD, FT-IR, DSC, and 1 H-NMR spectra. Molecular docking simulation found that Flu was imbedded into the β-CD cavity, folded, and centrally aligned inside. In addition, Flu/β-CD displayed increased soil mobility, sustained release behavior, and a 2.75-fold increase of the fungicidal activity against Botrytis cinerea . Importantly, the prepared inclusion complex exhibited decreased acute toxicity to phytoplankton ( Chlorella vulgaris ), zooplankton ( Daphnia magna ), and fish ( Danio rerio ) than technical Flu. This study provided useful information for the rational use of Flu in agriculture, as well as the reduction of ecological risk to the aquatic environment.
研究领域:
农业科学
·
环境科学
·
药物化学
✓ 已验证
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Pan-Pan Guo,Xi-Bin Yang,Hong Yang,Cao Zhou,Gui-Yun Long,Dao-Chao Jin
β -N-Acetylglucosaminidases and/or β - N -acetylhexosaminidases (NAGs / Hexes) are crucial exonucleases, playing a crucial role in the insect molting process. SfHex3 and SfHex4 contain conserved catalytic domains of GH20 and GH20b, clustered into NAG2 and NAG1 group, respectively. SfHex3 and SfHex4 were mainly highly expressed in the 4th–5th instar nymphs, as well as in the integument and ovary. The expression level of SfHex3 gradually decreased in male and female adults, and SfHex4 on the first day of female was significantly higher than that on the first day of male. In addition, RNA interference (RNAi) results demonstrated that the downregulation of SfHex3 and SfHex4 expression in 5th-instar nymphs resulted in failed molting, and a high mortality. Furthermore, after RNAi with SfHex3 and SfHex4 , the transcript levels on key genes of the chitin metabolism pathway ( SfCHS1 , SfCHS1a , SfCHS1b , SfTRE1 , SfTRE2 , SfCht5 , and SfCht7 ) were significantly decreased compared to the control group. Meanwhile the expression levels of SfHex3 and SfHex4 were up-regulated after 6 h and 12 h of 20E treatment. And the transcription levels of SfHex3 and SfHex4 were significantly inhibited at nitenpyram LC 20 , LC 50 , and LC 90 after 96 h of treatment, in 3rd nymphs of Sogatella furcifera . In conclusion, SfHex3 and SfHex4 play important roles in the nymphal development of S. furcifera , contributing to the molting process from nymph to adult. This study not only enhances our understanding of the nitenpyram in pest control, but also provides a foundation for the development of new control strategies using RNAi to targeting SfHex3 and SfHex4 .
研究领域:
昆虫学
·
分子生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）