ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Jia-Yi Zheng,Xiao-Yuan Ji,An-Qi Zhao,Fang-Yuan Sun,Li-Fang Liu,Gui-Zhong Xin
Mitochondrial complex activity controls a multitude of physiological processes by regulating the cellular metabolism. Current methods for evaluating mitochondrial complex activity mainly focus on single metabolic reactions within mitochondria. These methods often require fresh samples in large quantities for mitochondria purification or intact mitochondrial membranes for real-time monitoring. Confronting these limitations, we shifted the analytical perspective toward interactive metabolic networks at the whole-cell level to reflect mitochondrial complex activity. To this end, we compiled a panel of mitochondrial respiratory chain-mapped metabolites (MRCMs), whose perturbations theoretically provide an overall reflection on mitochondrial complex activity. By introducing N-dimethyl-p-phenylenediamine and N-methyl-p-phenylenediamine as a pair of mass spectrometry probes, an ultraperformance liquid chromatography-tandem mass spectrometry method with high sensitivity (LLOQ as low as 0.2 fmol) was developed to obtain accurate quantitative data of MRCMs. Machine learning was then combined to capture the relationship between MRCMs and mitochondrial complex activity. Using Complex I as a proof-of-concept, we identified NADH, alanine, and phosphoenolpyruvate as metabolites associated with Complex I activity based on the whole-cell level. The effectiveness of using their concentrations to reflect Complex I activity was further validated in external data sets. Hence, by capturing the relationship between metabolites and mitochondrial complex activity at the whole-cell level, this study explores a novel analytical paradigm for the interrogation of mitochondrial complex activity, offering a favorable complement to existing methods particularly when sample quantities, type, and treatment timeliness pose challenges. More importantly, it shifts the focus from individual metabolic reactions within mitochondria to a more comprehensive view of an interactive metabolic network, which should serve as a promising direction for future research into the functional architecture between mitochondrial complexes and metabolites.
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Journal of Energy Storage
·
2024
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IF 8.9
Meichen Guo,Wu Tang,Chenbin Tang,Xuesong He,Cong Fan
Organic cathodes face the challenge of dissolution problem, which severely hinders their electrochemical performances in lithium-ion batteries (LIBs). Herein, a bipolar organic cathode namely [ N , N ′-bis(4-(9H-carbazol-9-yl)phenyl)]-perylene-3,4,9,10-tetracarboxydiimide (PTCDI-DPC, 4-electron C T = 123 mAh g −1 ) is optimized by in-situ electropolymerization to eliminate its dissolution problem, and thus the good cathode performance of the electropolymerized PTCDI-DPC can be fully released in LIBs. In half cells, PTCDI-DPC delivers a peak specific capacity of 164 mAh g −1 and the good retention of 96 % over 450 cycles. Meanwhile, PTCDI-DPC still remains 121 mAh g −1 after 2000 cycles at 1 A g −1 , and a superior rate performance of 98 mAh g −1 at 8 A g −1 . Furthermore, the fabricated 2PF 6 -PTCDI-DPC||LiC 6 Li-ion full cells can possess the peak discharge capacity of 157 mAh g −1 cathode and a high energy density of 385 Wh kg −1 cathode in 1.4–4.3 V, with 90 % capacity retention during 2000 cycles at 1 A g −1 . As far as we know, the cathode performance of the improved PTCDI-DPC is one of the best among bipolar organic cathodes reported for LIBs.
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ChemistrySelect
·
2024
·Vol. 9
·期 30
·e202402251
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IF 1.9
Lihua Zhu,Ziying Huang,Tianhao Ge,Chaoqi Jiang,Wei Zhong,Palanisamy Kannan
Graphical The incorporation of phenolic hydroxyl group, pyridine spacer and Cl − into imidazolium-based ionic polymer led to enhanced catalytic activity of CO 2 conversion under mild conditions. In this study, imidazolium-based multifunctional ionic polymer ( IP 1 – IP 3 ) series, co-incorporated with phenolic hydroxyl groups as hydrogen bond donors (HBDs) and Cl − as nucleophiles, are synthesized by a facile quaternization method. The physicochemical characterizations of these IPs are systematically examined by using FTIR, XPS, BET, TGA, CO 2 -TPD, SEM with elemental mapping and TEM methods. Their catalytic activities are evaluated toward the conversion of carbon dioxide (CO 2 ) and epoxides into cyclic carbonates under solvent- and additive-free environments. Apart from the synergy effect between HBDs and Cl − , it is found that the types of the spacer linking the two imidazole units in the diimidazole precursors ( L 1 – L 3 ) also play an important role in the catalytic activity. And IP 2 , with a pyridine spacer as a Lewis base site, exhibits the best catalytic performance under solvent-free mild conditions i. e., atmospheric pressure CO 2 , 80 °C, and 5 h. Notably, the catalyst demonstrates a good substrate applicability. Furthermore, IP 2 exhibited good reusability, stability, and it can be recycled for ten successive runs with stable and potential catalytic activity. This study provides an alternative route to construct IPs with efficient activity for CO 2 catalytic conversion and fixation under mild-conditions.
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Cell Metabolism
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2024
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IF 27.7
Rui Li,Yan Li,Kun Jiang,Lijuan Zhang,Ting Li,Aihua Zhao,Zhuo Zhang,Yale Xia,Kun Ge,Yaqiong Chen,Chengnuo Wang,Weitao Tang,Shuning Liu,Xiaoxi Lin,Yuqin Song,Jie Mei,Chun Xiao,Aoxue Wang,Yejun Zou,Xie Li,Xianjun Chen,Zhenyu Ju,Wei Jia,Joseph Loscalzo,Yu Sun,Wei Fang,Yi Yang,Yuzheng Zhao
Arginine is one of the most metabolically versatile amino acids and plays pivotal roles in diverse biological and pathological processes; however, sensitive tracking of arginine dynamics in situ remains technically challenging. Here, we engineer high-performance fluorescent biosensors, denoted sensitive to arginine (STAR), to illuminate arginine metabolism in cells, mice, and clinical samples. Utilizing STAR, we demonstrate the effects of different amino acids in regulating intra- and extracellular arginine levels. STAR enabled live-cell monitoring of arginine fluctuations during macrophage activation, phagocytosis, efferocytosis, and senescence and revealed cellular senescence depending on arginine availability. Moreover, a simple and fast assay based on STAR revealed that serum arginine levels tended to increase with age, and the elevated serum arginine level is a potential indicator for discriminating the progression and severity of vitiligo. Collectively, our study provides important insights into the metabolic and functional roles of arginine, as well as its potential in diagnostic and therapeutic applications.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2024
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IF 11.2
Bo Zhang,Kang Li,Renfu Li,Shoufeng Wang,Longtian Kang
Here, 1D bis(N-carboxymethyl) peryleneimide (H 2 PDI), 0D 1,6,7,12-tetrachloro-bis(N-carboxymethyl) peryleneimide (4Cl-H 2 PDI), and 2D 4Cl-H 2 PDI/graphene quantum dot (4Cl-H 2 PDI/GQD) nanostructures are synthesized and carefully analyzed. The effect of bay-/end-substitution and S-scheme heterojunction of PDI-based materials as main catalysts on the photocatalytic H 2 O 2 evolution is first studied through the oxygen reduction reaction (ORR). Under the visible-light irradiation (> 420 nm), 4Cl-H 2 PDI and 4Cl-H 2 PDI/GQD as photocatalysts exhibit the ∼7 and ∼16 times H 2 O 2 evolution rate than H 2 PDI (1059.6 vs. 2484.0 vs. 160.0 µM g −1 h −1 ), respectively. The systematical experiments reveal that 4Cl-H 2 PDI and 4Cl-H 2 PDI/GQD should prefer a two-step single-electron ORR process, while H 2 PDI may involve a 4e ‒ water oxidation and one-step 2e ‒ ORR process. Further experiments confirm that the bay-substitution and GQD doping of H 2 PDI can promote the generation, transportation, and separation of photogenerated electrons and holes, and prolong the carrier lifetime. This work provides insight into PDI-based photocatalytic H 2 O 2 production.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Ning Li,Chao Ma,Dudu Li,Peng Li,Mao Ye,Ziyue Wang,Zhihua Qiao,Chongli Zhong
Mixed matrix membranes (MMMs), combining the advantages of polymer membranes and porous materials, processing the potential to realize large-scale gas separation applications. However, the interfacial defects in MMMs greatly hindered gas separation performance improvement. Therefore, designing an MMM with high loading and good interfacial compatibility remains a critical issue in promoting the application of MMMs. Herein, during in - situ heating process, the melting of liquid ZIF-62 in PBI polymer can largely fill the interfacial defects in MMMs. The microporous transport channels of ZIF-62 glass in MMMs promoted the H 2 diffusion and inhibited the CO 2 and CH 4 diffusion, thus enhancing the H 2 /CO 2 and H 2 /CH 4 separation of MMMs. For mixed gas testing, the 45 wt% ZIF-62 glass/PBI membrane had the maximum H 2 permeability of 459 barrer, and its H 2 /CO 2 and H 2 /CH 4 selectivities were 23 and 57, higher than the gas separation upper bonds. In addition, ZIF-62 glass/PBI membrane exhibited stable separation performance under 1–5 bar pressure. Therefore, the rational combination of MOF glass and polymer matrix could provide promising potential for reducing the interfacial defects of high-loading MMMs, thus enhancing the gas separation performance.
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CHEMICAL ENGINEERING SCIENCE
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2024
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IF 4.1
Haiyan Jiang,Bingbing Yang,Kuilin Peng,Zetao Liu,Shaojuan Zeng,Xiangping Zhang,Lu Bai
The combination ionic liquids (ILs) with membranes is a potential method for direct NH 3 separation. In particular, ILs can modulate the interactions with NH 3 and construct pathways in membranes for preferential NH 3 transport, which is expected to overcome the trade-off effect and achieve a desirable separation performance. In this work, four metal ILs (MILs) with different metal centres and hydrogen bond donating ability, including [2-Mim][Li(NTf 2 ) 2 ], [Eim][Li(NTf 2 ) 2 ], [Bmim] 2 [Co(NCS) 4 ], and [Bim] 2 [Co(NCS) 4 ], were incorporated into sulfonated block copolymers to prepare MIL-based hybrid membranes for NH 3 separation. The investigation of structure–performance relationships suggested that the interaction sites and transport channels could be tuned by the MIL type, which greatly affects the NH 3 separation performance of the membranes. The Nexar/2-Mim-Li-10 membrane exhibits NH 3 permeability of 735.6 Barrer with NH 3 /N 2 selectivity of 844.5 and NH 3 /H 2 selectivity of 154.7, which is higher than other MIL-based hybrid membranes owing to self-assembled transport channels and moderate complexation. Increasing the MIL content and feed pressure significantly improved NH 3 separation performance.
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NEW JOURNAL OF CHEMISTRY
·
2025
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IF 2.5
Yafei Sang,Xiaolong Jia,Pengpeng Wu,Xiangquan Yan,Xiaotao Kuang,Quan Feng,Jiahao Qiao,Min Chen,Jian Zhang,Xiuli Yan,Tao Chang
The porous organic polymers HIP-Br-1 and HIP-Br-2 rich in bromine anions were prepared by quaternization one-pot method. The contents of N and Br in HIP-Br-1 reached 15.93 wt% and 6.94 wt% respectively. These high content of bromine ions combined with rich mesoporous and macroporous structures gave the materials good catalytic ability. The material has moderate adsorption heat (28-36 kJ/mol). The catalytic experiment shows that HIP-Br-1 has good catalytic performance. Under the mild conditions of 0.4 MPa CO2, 60 oC, and 9 h, without any cocatalyst and solvent, 93% of propylene oxide can be converted to cyclic carbonate. The catalyst has good repeatability, and the conversion efficiency to cyclic carbonate did not significantly decrease after four catalytic experiments. Density functional theory (DFT) shows that HIP-Br-1 can effectively activate PO and CO2, thereby enhancing the catalytic activity of cycloaddition reactions. This work explores the one-step synthesis of ion-type porous organic polymers rich in nitrogen atoms.
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JOURNAL OF MEMBRANE SCIENCE
·
2025
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IF 9
Haiyang Shao,Zhengke Tan,Yafei Cheng,Xiang'an Yue,Lei Yuan,Weixiong Jian,Longqiang Xiao,Xiaocheng Lin
Diffusion dialysis (DD) presents a highly promising and cost-efficient approach for acid recovery, yet its performance is often hindered by the acid permeability of anion exchange membranes (AEMs). To address this challenge, a one-step approach has been developed to fabricate porous AEMs. Using brominated polyphenylene oxide (BPPO) as the porous base membrane, imidazole is employed for in-situ crosslinking and cationization. This approach not only retains the inherent porosity (>63.5 %) of the base membrane but also achieves an exceptionally thin selective layer (<0.3 μm), thereby creating abundant free space volume. Through a concurrent enhancement of ion transport sites and crosslinking density, the membrane effectively breaks acid permeability–selectivity trade-off. The optimal IPPO-20h AEM demonstrates a remarkable increase in both the acid dialysis coefficient ( U H + = 34.1 × 10 −3 m/h) and separation factor ( S H + / F e 2 + = 573.8) in the HCl/FeCl 2 system, with enhancements of 4- and 31-fold, respectively, compared to the commercial DF120 AEM, at 25 °C. This high-performance AEM, synthesized via a straightforward and mild process, offers significant potential for large-scale acid recovery applications.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Tao Peng,Xu Lu,Yang Jin,Xicheng Yang,Qian Liu,Chen Chen,Wanting Zhou,Yuting Guo,Yu Zhang,Yankun Gao,Hongjuan Zhang,Hongliang He,Lina Chen
It is effective through uncoupling postsynaptic density protein-95 (PSD95) and neuronal nitric oxide synthase (nNOS) for treating ischemic stroke with high disability, mortality and recurrence rate. However, there are no reliable uncouplers for PSD95-nNOS in clinical practice, and the established screening models for discovering PSD95-nNOS uncouplers have high costs and interference. In this study, epoxy functional fluorescent magnetic mesoporous silica (EFMMS) was synthesized by combining carbon dots (CDs), magnetic mesoporous silica and epoxy functionalization. Ni-EFMMS was further constructed by loading nickel ions to simultaneously purify and covalently immobilize His-tagged neuronal nitric oxide synthase (His-nNOS) and then couple with green fluorescent proteins (GFP) labeled PSD95, finally fabricating nano-biosensor (PSD95-nNOS/Ni-EFMMS). The nanomaterial was well constructed and characterized, and it distinctly constituted stable ratio fluorescence between CDs and GFP-PSD95. The ratio fluorescence intensity of PSD95-nNOS/Ni-EFMMS remained stable even after separately mixing with metal ions, amino acids and natural impurities, and the loss of ratio fluorescence intensity was less than 20% even at pH 4 or 10. PSD95-nNOS/Ni-EFMMS still kept the ratio fluorescence intensity above 90% after being recycled for seven times. Three compounds (gnetol, baicalein and emodin-8- O -β- D -glucoside) with strong uncoupling efficiency were screened from fourteen natural active ingredients. Furthermore, the theoretical feasibility of uncoupling was verified by molecular docking. In general, this study provides a novel “one-stone-four-birds strategy” to fabricate magnetic mesoporous nano-ratio fluorescence sensor for efficiently and accurately discovering PSD95-nNOS uncoupling active substances.
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POLYMER INTERNATIONAL
·
2025
·
IF 3.6
Liyue Zhang,Jingkai Liu,Xiaoling Liu,Jinyue Dai,Xiaoqing Liu,Xiaosu Yi
The flame-retardant modification of plant fiber-reinforced composites (PFRCs) is a fundamental requirement for achieving broader industrial applications. However, commonly used modification methods often compromise the fiber mechanical properties or surface reactivity due to structural damage. In this paper, a water-soluble system consisting of phytic acid imidazole salt and biobased benzoxazine was devised to modify a regenerated cellulose fabric through water-phase immersion. Benefiting from the neutral solution and self-assembly coating, the modified fabric (F-RCF) exhibited a typical self-extinguishing behavior while maintaining good structural integrity and mechanical properties. By further combining F-RCF with a DOPO-containing epoxy resin blend (FEB) to prepare a fiber-reinforced green composite (F-RCF/FEB), the results indicated that the co-curing reaction between the modifier and resin matrix contributed to improved interfacial adhesion. This enabled the simultaneous enhancement of tensile modulus (from 9.82 to 11.29 MPa), impact strength (from 6.58 to 7.26 kJ m −2 ) and interlaminar shear strength (from 11.41 to 13.89 MPa). More importantly, F-RCF/FEB exhibited excellent anti-flammability in terms of a high limiting oxygen index value of 34.3% and a UL-94 V-0 rating, and the peak heat release and total heat release were also markedly reduced by 44.9% and 36.9%, respectively. Mechanism analysis revealed that the modified system effectively prevented the wick effect and provided an inhibitory effect in the gaseous phase, as well as a barrier effect in the condensed phase. This study presents a non-destructive modification strategy for plant fibers, which may also inspire the synchronous enhancement of interfacial compatibility and fire-proof performance of PFRCs. © 2025 Society of Chemical Industry.
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JOURNAL OF INORGANIC BIOCHEMISTRY
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2025
·
IF 3.2
Hao Wang,Guozhu Ren,Yue Xu,Ruiping Deng,Rui Wang,Liang Zhou
In this work, novel erbium complex with anticancer activity against radiation resistant lung adenocarcinoma cells was obtained and demonstrated. Firstly, stronger inhibitory effect of Er 3+ on non-small cell lung cancer (NSCLC) cells and NSCLC- radiation resistant (RR) cells was experimentally confirmed. Then, by selecting highly biocompatible porphyrins as ligands, a novel erbium complex tetraphenylporphyrin erbium acetylacetonate (Er(acac)TPP) was synthesized and purified. Compared with Cisplatin, notably, Er(acac)TPP exhibits relatively higher inhibitory efficiency on NSCLC-RR cells. Moreover, the toxicities of Er(acac)TPP to normal cells are much lower than that of cancer cells. Subsequently, cell expansion, increased apoptosis, a decline in mitochondrial membrane potential (MMP), an accumulation of intracellular reactive oxygen species (ROS), increased Caspase-9 protein level and G2/M arrest were seen. These data all pointed to Er(acac)TPP as a possible candidate for more research and development as a chemotherapeutic drug.
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