KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2018
·Vol. 35
·期 6
·1312-1318
·
IF 2.199
Xiong Caixia,Sun Yong,Du Juan,Chen Wei,Si Zhihao,Gao He,Tang Xing,Zeng Xianhai
5-[(Formyloxy)methyl]furfural (FMF), an analogue of 5-(hydroxymethyl)furfural (HMF) is becoming more attractive due to its superior stability and hydrophobicity, which make it easier to refineand store. In the present study, FMF was produced from fructose by one-pot approach in pure formic acid media or by a two-step approach via HMF in choline chloride (ChCl)/fructose deep eutectic solvents (DES) system. A favorable FMF yield of 63.22% was reached by two-step approach. In addition, the effects of reaction parameters, such as temperature and acidity, on preparation of FMF from fructose were systematically investigated. The dehydration of fructose into HMF was confirmed as the rate-controlling step in the consecutive reaction. Ultimately, the separation and purification procedures of FMF were put forward. The FMF with a purity of 98.8% was obtained finally. Meanwhile, the FMF purified by saturated sodium bicarbonate solution showed an excelled storage stability.
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Biochimica et Biophysica Acta-Gene Regulatory Mechanisms
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2018
·
IF 5.179
Quan Gan,Xinwei Zhang,Daobo Zhang,Liang Shi,Yue Zhou,Tongtong Sun,Song Jiang,Junshan Gao,Yan Meng
Sucrose is the most commonly transported sugar in plants and is easily assimilated by insects to fulfill the requirement of physiological metabolism. BmSuc1 is a novel animal β-fructofuranosidase (β-FFase, EC 3.2.1.26) - encoding gene that was firstly cloned and identified in silkworm , Bombyx mori . BmSUC1 was presumed to play an important role in the silkworm-mulberry enzymatic adaptation system by effectively hydrolyzing sucrose absorbed from mulberry leaves. However, this has not been proved with direct evidence thus far. In this study, we investigated sucrose hydrolysis activity in the larval midgut of B. mori by inhibition tests and found that sucrase activity mainly stemmed from β-FFase, not α-glucosidase. Next, we performed shRNA-mediated transgenic RNAi to analyze the growth characteristics of silkworm larvae and variations in glycometabolism in vivo in transgenic silkworms. The results showed that in the RNAi- BmSuc1 transgenic line, larval development was delayed, and their body size was markedly reduced. Finally, the activity of several disaccharidases alone in the midgut and the sugar distribution, total sugar and glycogen in the midgut, hemolymph and fat body were then determined and compared. Our results demonstrated that silencing BmSuc1 significantly reduced glucose and apparently activated maltase and trehalase in the midgut. Together with a clear decrease in both glycogen and trehalose in the fat body, we conclude that BmSUC1 acts as an essential sucrase by directly modulating the degree of sucrose hydrolysis in the silkworm larval midgut, and insufficient sugar storage in the fat body may be responsible for larval malnutrition and abnormal petite phenotypes.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-8
·
IF 5.705
Chen Ying-Chu,Hsu Jui-Hung,Hsu Yu-Kuei
This study introduces a two-step method for the deposition of branched silver nanowires (AgNWs) on fluorine-doped tin oxide (FTO) glass. This material serves as both an active surface-enhanced Raman-scattering (SERS) substrate and as an enzyme-free electrochemical sensor for H 2 O 2 . This dual functionality is systematically studied. The AgNWs as the main trunk were first deposited on FTO by spray-coating. Silver branches were then electrochemically produced on the preformed NWs. Scanning electron microscopy, X-ray diffraction and X-ray photoelectron spectrometry were employed to characterize morphology, composition and microstructure. SERS experiments show that the branched AgNW/FTO substrate exhibits excellent performance in detecting 4-aminothiophenol at an ultra-low concentration of 0.1 fM. Simultaneously, this material displays an excellent electrocatalytic response to H 2 O 2 reduction at a concentration as low as 1 μM. The sensor has a rapid response and two linear analytical ranges that extend from 0.25 to 300 μM, and from 0.3 to 2.6 mM of H 2 O 2 , respectively. The ultrahigh sensitivity and satisfactory reproducibility highlights the merit of this hierarchical AgNW dendritic structure for sensing applications.
Branched silver nanowires can serve as both an active surface-enhanced Raman scattering substrate and as an electrochemical sensor for H 2 O 2 . This dual functionality is systematically investigated.
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Journal of Energy Chemistry
·
2018
·
IF 3.886
Huai Liu,Xing Tang,Weiwei Hao,Xianhai Zeng,Yong Sun,Tingzhou Lei,Lu Lin
In this contribution, one-pot tandem conversion of fructose into biofuel components, including 5-ethoxymethylfurfural (EMF), 2,5-( bis (ethoxymethyl)furan (BEMF) and ethyl levulinate (EL), was performed in an in-situ generated catalyst system through consecutive dehydration, etherification , and transfer hydrogenation. Specifically, ZrOCl 2 ⋅8H 2 O was in-situ decomposed into HCl and ZrO(OH) 2 in ethanol, which effectively catalyzed the dehydration/etherification of fructose to 5-ethoxymethylfurfural (EMF) and subsequent reductive etherification of EMF using ethanol as H-donor, respectively. EMF, BEMF and EL were detected as the main products, and total yield of detectable products of up to 65.4% was obtained at 200 °C in only 2 h.
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SENSORS
·
2018
·Vol. 18
·期 8
·2525
·
IF 2.475
Zhengjun Huang,Linlin Zheng,Feng Feng,Yuyuan Chen,Zhenzhen Wang,Zhen Lin,Xinhua Lin,Shaohuang Weng
Simple and effective methods for the detection of the level of blood glucose are closely linked to the monitoring of people’s health. In the study, MnO2nanosheets with absorption range of 300 nm~500 nm and obvious yellow color were easily prepared and applied to detect glucose through their absorbance and color. The proposed method is based on the fact that a specific concentration of glucose can be quantitatively transformed into hydrogen peroxide (H2O2) under the catalytic effect of glucose oxidase. Based on the redox reaction of MnO2with H2O2, yellow MnO2can be converted into colorless Mn2+to monitor the concentration of glucose. Under optimal conditions, a simple and effective visual assay for the sensitive and reliable detection of glucose was developed. The linear range was estimated to the range from 0 μM to 100 μM, with a detection limit of 12.8 μM. Furthermore, the proposed colorimetric assay based on MnO2nanosheets can effectively detect blood glucose of clinical serum samples with accuracy and convenience.Keywords:glucose;colorimetric assay;MnO2nanosheets;UV-vis;quantitative determination
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SENSORS
·
2018
·Vol. 18
·期 7
·2151
·
IF 2.475
Fei Qu,Jingwen Li,Wenli Han,Lian Xia,Jinmao You
Both adenosine triphosphate (ATP) and glucose are important to human health, and their abnormal levels are closely related to angiocardiopathy and hypoglycaemia. Therefore, the simultaneous determination of ATP and glucose with a single test mode is highly desirable for disease diagnostics and early recognition. Herein, a new fluorescence on/off switch sensing platform is developed by carbon nanodots (CNDs) to detect ATP and glucose simultaneously. The fluorescence of CNDs can be quenched by Cu2+and hydrogen peroxide (H2O2), due to the formation of hydroxyl radicals (·OH) produced in the Cu-Fenton reaction. Based on the high affinity of Cu2+with ATP, the fluorescence of CNDs will recover effectively after adding ATP. Additionally, glucose can be efficiently catalyzed by glucose oxidase (GOx) to generate H2O2, so the platform can also be utilized to analyze glucose. Under optimum conditions, this sensing platform displays excellent sensitivity and the linear ranges are from 0.1 to 7 μM for ATP with a limit of detection (LOD) of 30.2 nM, and from 0.1 to 7 mM for glucose with a LOD 39.8 μM, respectively. Benefiting from the high sensitivity and selectivity, this sensing platform is successfully applied for simultaneous detection of ATP and glucose in human serum samples with satisfactory recoveries.Keywords:simultaneous detection;Cu-Fenton reaction;carbon nanodots;adenosine triphosphate;glucose;satisfactory recoveries
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GREEN CHEMISTRY
·
2019
·Vol. 21
·期 14
·3930-3939
·
IF 9.405
Guo Qiu,Chongpin Huang,Xiuliang Sun,Biaohua Chen
Bio-based platform chemical 5-hydroxymethylfurfural (5-HMF) can be converted into large-scale, high value-added compounds generally obtained from petroleum. Hence, developing an efficient catalytic system to transform glucose (a cheap and abundant branch of biomass) into 5-HMF has drawn significant attention. Earlier reports have concentrated on a two-step conversion of glucose to 5-HMF involving glucose isomerization into fructose and subsequent dehydration. In this work, efficient heterogeneous niobium-loaded montmorillonite (Nb-MMT) catalysts containing Lewis and Brønsted acid sites have been synthesized by a readily cation-exchanged synthetic method and were used to convert glucose and showed high selectivity and conversion. An optimum 5-HMF yield of 70.52% with a 99% conversion of glucose was achieved in a biphasic solvent of methyl isobutyl ketone (MIBK)/water at 170 °C for 3 h. The catalytic system also exhibited an excellent activity in the conversion of disaccharides and polysaccharides to 5-HMF with satisfactory yields. In addition, Nb-MMT was effectively reused four times without significant loss of activity. Nb-MMT also featured good catalytic activity and selectivity towards carbohydrate conversion in water. Furthermore, the reaction mechanism for the transformation of glucose to 5-HMF over Nb-MMT was investigated by density functional theory (DFT) calculations, and the results showed that niobium oxyhydroxide species of Nb-MMT played an essential role in glucose conversion, and the catalytic mechanism was a synergetic proton transfer process via hydrogen bonding. This study paves the way for improvements in Nb-containing solid acid catalysts for conversion of carbohydrates.
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Applied Materials Today
·
2019
·
IF 8.013
Jiachen Ge,Xuanyu Yang,Jiahang Luo,Junhao Ma,Yidong Zou,Jichun Li,Wei Luo,Xiaowei Cheng,Yonghui Deng
With increasing requirements of replacing the high-cost and unstable natural enzymes, the rational design of highly efficient inorganic mimetic enzymes (nanozymes), as one of the most important bioactive materials, has attracted more and more attention in recent years. In our work, using the solvent evaporation induced co-assembly strategy with high-molecular weight poly(ethylene oxide)-block-polystyrene (PEO- b -PS) diblock copolymer as the template and two metal salts as the co-precursors in one-pot system, we synthesized ordered mesoporous heteroatom-doped CeO 2 with high surface area, bimodal pore sizes and highly crystallized walls. The composite materials were then constructed as the mimetic enzymes to investigate their peroxidase-like catalytic performance and detect H 2 O 2 and glucose as bioactive materials in diverse systems including complex biological samples. It was demonstrated that mesoporous 5%CoO/CeO 2 catalyst was the best peroxidase mimic, following the typical Michaelis–Menten kinetics and exhibiting the excellent peroxidase-like catalytic performance toward 3,3′,5,5′-tetramethylbenzidine (TMB) with comparable K m and V m values to horseradish peroxidase (HRP) enzyme. A highly sensitive and selective colorimetric assay was developed to detect H 2 O 2 and glucose with detection limits of 0.88 and 19.6 μM, respectively. The promising catalytic behavior of mesoporous 5%CoO/CeO 2 as the peroxidase-like bioactive catalyst could be ascribed to its interconnected large mesopores, rich exposed surface defects and oxygen vacancies introduced by the heterojunction interface of ultrasmall CeO 2 and CoO nanocrystals within pore walls.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-10
·
IF 5.479
Zhu Yanyan,Zhang Xuan,Sun Jiameng,Li Meng,Lin Yulong,Kang Kai,Meng Yang,Feng Zhongliang,Wang Jing
Ultrafine platinum nanoparticles are grown on a 3D graphene framework (GF–Pt) via a hydrothermal method. The material, when placed on a glassy carbon electrode (GCE), displays enhanced electrocatalytic activity towards glucose oxidation. This is assumed to be the result of the numerous easily accessible active sites, an enlarged electrochemically active area, and the presence of multiple electron/ion transport channels. The modified GCE can be operated at a low potential (− 0.15 V vs. Ag/AgCl) has linear responses in the 0.1 μM − 0.01 mM and 0.01 mM – 20 mM glucose concentration range, and a 30 nM detection limit. It was applied to the rapid determination of glucose in human serum samples.
Schematic presentation of a glassy carbon electrode modified with ultrafine Pt nanoparticles grown on a graphene framework (GFs–Pt). GFs–Pt presents enhanced electrocatalytic activity towards glucose oxidation. GFs–Pt is used in a sensitive non-enzymatic amperometric glucose sensor.
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TALANTA
·
2019
·
IF 4.916
Yaxue Jia,Shucheng Sun,Xiujun Cui,Xiaohong Wang,Li Yang
We show that vanadomolybdophosphoric heteropoly acid (H 5 PMo 10 V 2 O 40 , PMoV 2 ), one of the polyoxometalates (POMs), presents peroxidase-like activity that can catalyze the luminol/H 2 O 2 reaction to generate chemiluminescence (CL) emission. Based on this foundation, we report an enzyme-free luminol/H 2 O 2 /PMoV 2 CL system that can be utilized for the ultrasensitive detection of H 2 O 2 . The CL intensity exhibits good linear dependence on H 2 O 2 concentration in a wide range up to 5000 nM with a limit-of-detection of 5 nM. The method was successfully used for the determination of glucose in human serum. Our study paves the way for the application of functional POM materials in a variety of biosensing assays, such as the determination of cholesterol, uric acid , lactate, etc., in which H 2 O 2 is a product of oxidoreductase enzymes.
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ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Changhui Zhu,Haiyong Wang,Chilu Cai,Kang Bi,Bijuan Cai,Xiangbo Song,Qiying Liu,Longlong Ma
Biomass-derived 2,5-dimethylfuran (DMF) is an ideal, renewable gasoline additive, and its production with high productivity is highly desirable. A continuous production route was developed to yield DMF from fructose via a tandem strategy, where dehydration catalysts (HY zeolite and niobium phosphate) assisted by 1-butyl-3-methylimidazolium chloride ([BMim]Cl) and hydrodeoxygenation (HDO) catalyst Cu–Ru/C were integrated into one reaction system with γ-butyrolactone (GBL) as the mobile phase. Optimum conditions, such as temperature, H2 pressure, weight hourly space velocity, and [BMim]Cl concentration, were investigated systematically, with an initial HDO study by using 5-hydroxymethylfurfural (HMF) as substrate and subsequent tandem dehydration and HDO to produce DMF with fructose as substrate. Among the conditions, the highest yield of DMF at 55.2% was gained by niobium phosphate and Cu–Ru/C, with the aid of [BMim]Cl in a fixed bed. Meanwhile, [BMim]Cl facilitated fructose dehydration to HMF and also mediated HDO of the resultant HMF by stabilization, which was clarified by 1H NMR and FTIR spectroscopy. Finally, the fact that carbon deposit led to catalyst deactivation was examined thoroughly via a series of characterization techniques. This work achieved continuous DMF production from fructose and laid a foundation for future possible amplification of DMF.
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Analytical Methods
·
2019
·Vol. 11
·期 37
·4835-4841
·
IF 2.378
Wenbin Chen,Siyuan Wu,Jianing Zhang,Fengjie Yu,Xiaoqing Miao,Xijuan Tu
5-Hydroxymethylfurfural (HMF) is an important index for evaluating the quality of honey. In the present study, the recovery of HMF via the salting-out-assisted liquid–liquid extraction (SALLE) was systematically investigated to develop a simple, rapid, and effective method for the determination of HMF in honey. Results indicated that at the concentration of 70 : 30 (acetonitrile : H2O, v/v), sodium carbonate showed the best recovery of HMF from honey with the 0.5 g addition amount of salt. Compared with two other homogenous liquid–liquid extraction methods, i.e. subzero temperature-assisted liquid–liquid extraction and hydrophobic solvent-assisted liquid–liquid extraction, SALLE showed an advantage of higher recovery for HMF. In addition, fructose and glucose were analyzed to investigate the residue of sugars in the extractive. Results demonstrated that the SALLE method could dramatically reduce the residual contents of these two sugars. In addition, SALLE showed the advantages of less consumption of samples and extraction solvents. Finally, the developed method was fully validated and applied for real honey samples. The analysis results obtained using the present method were comparable to those obtained using the official method; this indicated that the proposed method could be used as an alternative method for the routine quality control of HMF in honey.
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