ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 13
·3091-3101
·
IF 3.637
Liu Yongquan,Xue Ahui,Ding Lili,Hao Yingbin,Liu Huihui,Cui Meng,Liu Lihua,Nie Zongxiu,Luo Liping
Citrus Huanglongbing (HLB) is one of the most destructive citrus diseases worldwide, and is associated with the phloem-limited plant pathogenic bacteria Candidatus Liberibacter species. However, there is a latency period during which newly infected trees do not show symptoms, creating challenges for the early detection of HLB. In order to establish a method for rapid detection and to assess the metabolite differences between healthy and HLB-affected Newhall navel oranges, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to study asymptomatic and symptomatic leaf extracts compared with healthy leaves. The results showed that healthy, asymptomatic, and symptomatic leaves were distinguishable using MALDI-TOF-MS combined with multivariable analysis. Discriminant analysis (DA) results showed that the accuracy of discrimination of the training set and the success rate of cross-validation analysis were both 100%. Blind testing revealed that nine validation samples were correctly classified to their corresponding group. In addition, 32 MS peaks of metabolites had significant differences between healthy and different HLB-affected navel orange leaves. Among 32 MS peaks, the relative content of nine substances showed an upward trend, and 19 metabolites showed a downward trend, including a significant decrease in malate and citrate, with an increase in HLB bacterial populations. Malate and citrate were found to be specifically reduced in infected leaves regardless of whether symptoms appeared. Thus, they could be used as potential biomarkers for early detection of HLB, reducing false-negative results caused by uneven distribution of the HLB bacterial pathogen. This novel method can efficiently differentiate HLB-affected samples from the host population and provide new information for early detection of HLB in citrus. The entire process from metabolite extraction to the collection of mass spectra of citrus leaves was a simple operation, with no need for sterile conditions. We believe this strategy has significant implications for rapid and effective HLB screening.
Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2020
·Vol. 10
·期 4
·631
·
IF 4.324
Zhen Li,Libo Yu,Hao Wang,Huiwen Yang,Huan Ma
Light harvesting and electron recombination are essential factors that influence photovoltaic performance of quantum dots sensitized solar cells (QDSSCs). ZnO hollow microspheres (HMS) as architectures in QDSSCs are beneficial in improving light scattering, facilitating the enhancement of light harvesting efficiency. However, this advantage is greatly weakened by defects located at the surface of ZnO HMS. Therefore, we prepared a composite hollow microsphere structure consisting of ZnO HMS coated by TiO2layer that is obtained by immersing ZnO HMS architectures in TiCl4aqueous solution. This TiO2-passivated ZnO HMS architecture is designed to yield good light harvesting, reduced charge recombination, and longer electron lifetime. As a result, the power conversion efficiency (PCE) of QDSSC reaches to 3.16% with an optimal thickness of TiO2passivation layer, which is much higher when compared to 1.54% for QDSSC based on bare ZnO HMS.Keywords:TiO2passivation layer;ZnO hollow microspheres;quantum dots sensitized solar cells
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2020
·
IF 7.183
Zhenhua Lü,Zhongwei Guo,Kaifei Zhang,Sanchuan Yu,Meihong Liu,Congjie Gao
This work focused on modification of polyamide based thin-film composite membrane via a novel strategy combining surface tertiary amination and zwitterionic functionalization for improved separation and anti-dye-deposition properties. Surface tertiary amination was conducted through grafting polyethyleneimine (PEI) onto the surface of base membrane at the sites of carboxylic groups activated by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. Zwitterionic functionalization was implemented through in situ ring-open reaction between tertiary amine groups of grafted PEI molecules and 1, 4-butanesultone. It was found that grafting of PEI could enhance CaCl 2 /NaCl rejection ratio from 0.36 to 3.05 through reversal of surface charge at a sacrifice of pure water permeation coefficient from 9.7 to 8.6 l/m 2 h bar. The following incorporation of zwitterions under desired condition could resume pure water permeation coefficient to a higher value of 12.6 l/m 2 h bar and further enhance CaCl 2 /NaCl rejection ratio to 3.11. Furthermore, proper surface tertiary amination and zwitterionic functionalization could even significantly improve membrane resistance to deposition by both cationic and anionic dyes. Membrane's water permeation coefficients to aqueous Congo red, Victoria blue B and methylene blue solutions under steady state were enhanced by 72.5, 78.8 and 53.2%, respectively, and relative flux decline ratios were lowered by 80.5, 80.3 and 79.0%.
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2020
·Vol. 13
·期 4
·839-849
·
IF 2.667
Zhou Ying,Cheng Fuliang,Hong Yanping,Huang Jianping,Zhang Xue,Liao Xiaoning
Isoproturon (ISO), an herbicide belonging to the phenyl urea family, is extensively used in modern agriculture to guarantee a bumper harvest. However, it has been evidenced that the ISO has heavy toxicity to the liver system and appears to be a tumor promoter. It is therefore urgent to develop sensitive and selective methods to screen its presence in food resources to meet the ever-increasing pollution restrictions. In this work, an electrochemical sensor based on graphene oxide–modified multi-walled carbon nanotubes hybrid material was firstly developed for the rapid and sensitive detection of ISO via square wave voltammetry (SWV). Under optimized experimental conditions, the sensor exhibits a relatively wide linear range from 0.30 to 15.0 μmol L −1 , a low limit of detection (LOD) of 0.10 μmol L −1 , as well as good repeatability, high reproducibility, excellent selectivity, and long-term stability. The sensor has also been successfully employed in various real samples such as rice field water, tomato, and lettuce analyses. The obtained recoveries are in the range of 97.33–105.0% with low relative standard deviation (less than 2.5%), which is very close to the results of the high-performance liquid chromatography (HPLC), showing the proposed electrochemical sensor has great potential in practical applications.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2020
·Vol. 24
·期 3
·583-590
·
IF 2.646
Yu Zhipeng,Kong Chuncai,Lv Jian,Ma Bo,Zhang Xiaojing,Yang Zhimao
Designing highly active material to fabricate high-performance glucose sensor was great importance for diabetes treatment. In this work, we develop a novel nanocomposite composed of multilevel nanostructure with ultrathin Cu x O nanoflakes anchored on Cu 2 O nanowires, directly on Cu foil and its application as non-enzymatic glucose sensor. Due to more active sites endowed by the ultrathin Cu x O nanoflakes, the as-prepared Cu x O nanoflakes/Cu 2 O nanowires@Cu nanoarray exhibit high sensitivity (3.59 mA mM −1 cm −2 ), low detection limit (0.69 μM), high selectivity, fast amperometric response (~ 2 s) and excellent reliability in human serum. And the sensitivity of ultrathin Cu x O nanoflakes/Cu 2 O nanowires@Cu nanoarray was 1.9-fold than that of Cu 2 O nanowires@Cu nanoarray. Such novel multilevel nanoarray synthesized by convenient method provides a facile way to design metal oxide nanomaterial for promising non-enzymatic glucose sensor.
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Tong Wang,Qing Wang,Ping Li,Hua Yang
The use of deep eutectic solvents (DESs) to extract natural products has attracted considerable attention in recent years. However, the recovery of extracted compounds and DES from DES extracts is still a barrier, which restricts the large-scale application of DESs. Here, we proposed a high-speed countercurrent chromatography (HSCCC)-based method to recover extracted compounds and DES. As a proof of concept, the chrysanthemum DES extract was selected to verify the method. We first prepared a series of DES-based solvent systems and screened them based on their physical and chemical properties. Only the system composed of choline chloride/l-malic acid, water, and ethyl acetate (1:1:1, v/v/v) was suitable. By using the solvent system for HSCCC separation, the target compounds and DES in the chrysanthemum DES extract were both successfully recovered with high recovery yields over 95%. Then, we investigated the reusability of the recovered DES and found that it could be repeatedly reused for three times without any performance deterioration. All these results indicated that the proposed method is feasible and efficient, which has great potential for application. Moreover, the method may also be used in other fields where the separation process is needed, such as organic synthesis, chemical separation, bioseparation, and biotransformation.
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
Zhuoli Xu,Kang Zhao,Fuping Li,Yashan Huo,Yufei Tang
In this paper, ZrB 2 –ZrC composite nanofibers were prepared by electrospinning method followed by carbothermal reduction process. Nanofibers with ZrC content of 15, 35 and 50 wt%, respectively, were obtained by varying the n(B)/n(Zr) molar ratio. The oxidation resistance of fabricated nanofibers was investigated by the non-isothermal thermogravimetric method. The effect of ZrC content on the oxidation resistance of ZrB 2 –ZrC composite nanofibers was analyzed systematically. The Kissinger method was used to calculate the oxidative activation energy (E) of the fibers with different ZrC content. The results show that the E values of fiber with n(B)/n(Zr) equal to 2.5, 3.5 and 4 are 219, 362 and 275 kJ/mol. Among the prepared fibers, the fiber with n(B)/n(Zr) of 3.5 has the highest oxidative activation energy and the best oxidation resistance.
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Yaoping Hu,Zhijin Gao
In this paper, we exploit an innovative strategy to reuse waste rubber tires as a low-cost source for the fabrication of a high-value material, fluorescent carbon dots (CDs). In the hydrothermal condition, ammonium persulphate is utilized to oxidize the tires and offer nitrogen atom for doping, to produce CDs with a high quantum yield (QY) of up to 23.8%. Such a QY is outstanding among the reported waste-derived CDs. It is found that the fluorescence of CDs can be remarkably quenched by Sudan I–IV with negligible interference from other substances. The corresponding linear ranges are 0.5–60, 0.5–60, 1–70, and 1–70 μM, and limits of detection are 0.17, 0.21, 0.53, and 0.62 μM for Sudan I, II, III, and IV, respectively. Systematic investigations reveal that the fluorescence quenching mainly stems from the inner filter effect. Moreover, the CD-based sensor shows an excellent applicability for the assay of Sudan dyes in chili powder sample.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Yingjie Wu,Luyao Ma,Qizhen Liu,Olivera Topalović,Qiong Wang,Xiaoe Yang,Ying Feng
Plant growth-promoting bacteria (PGPB) can promote root uptake and shoot accumulation of cadmium (Cd) in hyperaccumulator plants, but the mechanisms by which PGPB accelerate root-to-shoot transport of Cd is still unknown. A better understanding of these mechanisms is necessary to develop the strategies that can promote the practical phytoextraction of Cd-polluted soils. In this study, we found that Pseudomonas fluorescens accelerates a reversed and a long-distance transport of Cd and sucrose in Sedum alfredii , by examining the xylem and phloem sap and by quantifying the concentrations of Cd and sucrose in shoot and root. The transcriptome sequencing has revealed the up-regulated expressions of starch metabolism and sucrose biosynthesis related genes in the shoots of Cd hyperaccumulator plant S. alfredii that was inoculated with PGPB P. fluorescens . In addition, the genes of sugar, cation and anion transporters were also up-regulated by bacterial treatment, showing a complicated co-expression network with sucrose biosynthesis related genes. The expression levels of Cd transporter genes, such as ZIP1 , ZIP2 , HMA2 , HMA3 and CAX2 , were elevated after PGPB inoculation. As a result, the PGPB successfully colonized the root, and promoted the sucrose shoot-to-root transport and Cd root-to-shoot transport in S. alfredii. Since non-photosynthetic root-associated bacteria usually obtain sugars from photosynthetic plants, our results highlight the importance of PGPB-induced changes in hyperaccumlator plants for both the host and the PGPB.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2020
·Vol. 8
·期 13
·3741-3750
·
IF 6.183
Song Zhang,Yiyun Cheng
Cytosolic protein delivery technique plays an important role in protein-based biotechnologies and therapeutics. However, the development of efficient nanocarriers for delivering cargo proteins into cytosols remains a continuing challenge due to the existence of multiple barriers. Here, we report an efficient strategy for the cytosolic delivery of native proteins by surface-engineered gold nanoparticles combined with hypertonicity treatment. Sub-10 nm gold nanoparticles stabilized by both cysteamine and 4-mercaptophenylboronic acid were used to complex cargo proteins via a combination of nitrogen-boronate coordination and ionic interactions. The yielding protein complexes with a size around 100 nm showed efficient endocytosis via micropinocytosis- and lipid raft-mediated pathways. Further the hypertonicity treatment of the transduced cells by glycerol, glucose, sucrose, and NaCl solutions efficiently facilitates the endosomal escape and the intracellular release of cargo proteins. By the proposed strategy, cargo proteins including bovine serum albumin, ovalbumin, green fluorescent protein, R-phycoerythrin, and horseradish peroxidase were successfully delivered into cell cytosol with maintained protein bioactivity. This study provides a feasible and efficient strategy for the intracellular protein delivery.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Tao Han,Xiaoping Yu,Yafei Guo,Mingli Li,Ji Duo,Tianlong Deng
As a unique and indispensable element in modern battery and energy industries, the recovery of lithium has attracted wide attention worldwide. In this work, the recovery of low concentration of Li + in geothermal water was carried out by a green and novel FePO 4 /K 2 NiFe(CN) 6 [FPO/KNiFC] electrochemical ion pump technique. Different K 2 MFe(CN) 6 (M = Cu, Co, or Ni) was synthesized and used to construct an electrochemical ion pump with FePO 4 . It was found that K 2 NiFe(CN) 6 has excellent electrochemical properties, and the recovery of Li + is mainly restricted by the insertion capacity and rate of the FePO 4 electrode. This problem was successfully solved by the modification of electrode using multiwalled carbon nanotube . Even though the optimized ion pump system has a low single insertion capacity for low concentrations of Li + , a cycle extraction method was developed and successfully realized high-effective recovery of Li + from geothermal water. Subsequently, a multi-stage extraction device was designed and proposed for the continuous recovery of low concentrations of Li + .
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 23
·10022-10034
·
IF 3.553
Qu Fei,Yan Hang,Li Kexin,You Jinmao,Han Wenli
Covalent organic frameworks (COFs) are usually synthesized by virtue of Schiff base reaction between amino and aldehyde groups. Owing to their electron-rich property, COFs are widely applied to detect electron-deficient nitroaromatic explosives through photoinduced electron transfer mechanism. However, except for these cases, the COFs-based chemosensors are rarely reported for the analysis of electron-rich biomolecules. In this work, a fluorescent platform constructed by COFs and MnO 2 nanosheets is developed for sensitive detection of glutathione (GSH). It is found that the fluorescence of COFs can be effectively quenched by MnO 2 nanosheets. The quenching mechanisms involving inner filter effect and static quenching effect are discussed in detail. Due to the reducing ability of GSH, it can trigger the reduction of MnO 2 to Mn 2+ , accompanying with the fluorescence recovery of COFs. The linear range of GSH is achieved from 0.5 to 100 µM with a detection limit of 0.28 µM. This method is further applied for GSH detection in human serum samples with satisfactory results.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）