JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jingming Wang,Bin Wu,Na Zhang,Mingyue Zhao,Tingting Jing,Yi Wu,YunQing Hu,Feng Yu,Xiaochun Wan,Wilfried Schwab,Chuankui Song
β-Ionone is a carotenoid-derived flavor and fragrance compound with a complex fruity and woody scent, known for its violet aroma. Due to the low odor threshold, β-ionone dramatically affects the aroma and quality of tea. Previous studies have shown that β-ionone increases during tea withering; however, its formation and regulation during the withering process are far from being understood. As dehydration is the most important stress during the withering of the tea leaves, we isolated a dehydration-induced gene belonging to the subfamily of carotenoid cleavage dioxygenases called carotenoid cleavage dioxygenase 1a (CsCCD1a) from Camellia sinensis and expressed it in Escherichia coli. The recombinant protein could generate volatile β-ionone and pseudoionone from carotenoids. CsCCD1a was induced by dehydration stress, and its expression was related to the β-ionone accumulation during tea withering. Overall, this study elucidated that CsCCD1a catalyzes the formation of β-ionone in C. sinensis and enhanced the understanding of the β-ionone formation under multiple stresses during the processing of tea.
研究领域:
植物生物化学
·
农学
·
食品科学
·
分子生物学
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DOI
PubMed
高影响因子
CELLULAR AND MOLECULAR LIFE SCIENCES
·
2024
·Vol. 81
·期 1
·1-22
·
IF 6.2
Feng Chenxi,Yan Jinhua,Luo Ting,Zhang Hong,Zhang Hu,Yuan Yu,Chen Yi,Chen Haiyang
Inflammatory bowel diseases (IBDs) are immune chronic diseases characterized by recurrent episodes, resulting in continuous intestinal barrier damage and intestinal microbiota dysbiosis. Safe strategies aimed at stabilizing and reducing IBDs recurrence have been vigorously pursued. Here, we constructed a recurrent intestinal injury Drosophila model and found that vitamin B12 (VB12), an essential co-factor for organism physiological functions, could effectively protect the intestine and reduce dextran sulfate sodium-induced intestinal barrier disruption. VB12 also alleviated microbial dysbiosis in the Drosophila model and inhibited the growth of gram-negative bacteria. We demonstrated that VB12 could mitigate intestinal damage by activating the hypoxia-inducible factor-1 signaling pathway in injured conditions, which was achieved by regulating the intestinal oxidation. In addition, we also validated the protective effect of VB12 in a murine acute colitis model. In summary, we offer new insights and implications for the potential supportive role of VB12 in the management of recurrent IBDs flare-ups.
研究领域:
胃肠病学
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微生物学
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分子生物学
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营养学
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DOI
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 23
·2200300
·
IF 6.575
Zhaodi Zhang,Jiahong Li,Ming Zhang,Binbin Li,XiaoHua Pan,Xiaoliang Dong,Li-Long Pan,Jia Sun
Scope Dietary fibers can alter gut microbiota and microbial metabolite profiles. SCFAs are produced by bacterial fermentation of fiber, mediating immune homeostasis through G-protein-coupled receptors (GPCRs). GPR109a, a receptor for niacin and butyrate, expressed by immune cells and non-immune cells, is a key factor regulating immune responses. However, the role and underlying mechanisms of GPR109a in type 1 diabetes (T1D) remain unclear. Methods and results Experimental T1D was induced by streptozotocin in GPR109a-deficient ( Gpr109a −/− ) and wild type mice. The study found that Gpr109a −/− mice were more susceptible to T1D with dysregulated immune responses, along with increased M1 macrophage polarization (from 10.55% to 21.48%). Further, an adoptive transfer experiment demonstrated that GPR109a-deficient macrophages promoted the homing of intestine-derived type 1 cytotoxic T cells to pancreas (from 18.91% to 24.24%), thus disturbing the pancreatic immune homeostasis in non-obese diabetic mice. Mechanistically, GPR109a deficiency promoted M1 macrophage polarization associated with the activation of suppressor of cytokine signaling 3-signal transducer and activator of transcription 1 signaling pathway. Conclusion The findings reveal that macrophage GPR109a deficiency accelerates the development of T1D. Activation of GPR109a on macrophage by dietary components may provide a new strategy for preventing or treating T1D.
研究领域:
免疫学
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内分泌学
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代谢学
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胃肠病学
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DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2026
·
IF 3.4
Chen Licong,Shi Rui,Han Yunfei,Wang Limei,Li Xingyun,Wang Zhongzhi
1-Hydroxypyrene (1-HP) is the final metabolite of polycyclic aromatic hydrocarbons in the human body, and Fe 3+ is an important trace element for human growth and metabolism. When 1-HP or Fe 3+ is detected in human urine, it indicates that there is a problem with the human health condition. The detection of 1-HP and Fe 3+ in urine can reflect the health status of the human body. In this work, Eu(III) complexes coated silane coupling agent (WD-53) modified spherical SiO 2 core-shell composite material (SiO 2 -WD-53@L-Eu) was prepared and used as the fluorescent probe for detecting 1-HP and Fe 3+ . The fluorescence intensity of SiO 2 -WD-53@L-Eu with the same mass concentration is 1.37 times that of L-Eu, while the content of Eu 3+ in SiO 2 -WD-53@L-Eu is only about one-third of L-Eu. Moreover, the dispersion stability of SiO 2 -WD-53@L-Eu in solvents is much higher than that of L-Eu. Furthermore, SiO 2 -WD-53@L-Eu exhibits the sensitive response to 1-HP and Fe 3+ without interference from inorganic salts, urea, glucose and other components. The fluorescence quenching constants for detecting 1-HP and Fe 3+ are 9.62 × 10 5 M − 1 and 5.29 × 10 3 M − 1 , respectively, and the limits of detection are 5.93 × 10 − 6 M and 1.08 × 10 − 3 M, respectively. We further employed the SiO 2 -WD-53@L-Eu composite material within a Tris-HCl buffer to detect 1-HP and Fe 3+ , respectively, and obtained Stern-Volmer quenching constants (K sv ) of 3.662 × 10 5 M − 1 and 1.957 × 10 3 M − 1 . Compared to L-Eu, composite material SiO 2 -WD-53@L-Eu has higher fluorescence efficiency, better dispersion stability and chemical stability. Therefore, SiO 2 -WD-53@L-Eu is considered a promising material for detecting 1-HP and Fe 3+ in urine.
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产品关联
DOI
高影响因子
Scientific Reports
·
2026
·
IF 4.9
Dang Yanqing,Xiang Yanju,Abudula Adila,Hou Xujie,Pu Yunfeng
This study investigated the efficacy of γ-aminobutyric acid (GABA, 1.0 mM) and 1-methylcyclopropene (1-MCP, 1 µL L − 1 ), individually and in combination, in mitigating watercore disappearance, flesh browning, and postharvest quality in apples during 180 days storage. The results showed that the combined GABA + 1-MCP treatment outperformed individual applications, significantly delaying watercore disappearance by preserving sucrose and sorbitol while moderating glucose and fructose accumulation. Furthermore, the combined treatment significantly reduced flesh browning compared to the control, likely attributed to suppressed polyphenol oxidase activity and enhanced antioxidant capacity. This approach also minimized weight loss and maintained firmness and titratable acidity. Although total phenolics and total flavonoids increased progressively during storage, their levels were effectively regulated by the GABA + 1-MCP treatment. These results indicate that the combined exogenous application of GABA and 1-MCP is a potentially effective method to improve postharvest quality, and enhance storage efficiency of apple fruit.
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产品关联
DOI
高影响因子
BMC Veterinary Research
·
2023
·Vol. 19
·期 1
·1-11
·
IF 2.6
Gao Yafan,Shen Yu,Fan Jiyuan,Ding Haojie,Zheng Bin,Yu Haijie,Huang Siyang,Kong Qingming,Lv Hangjun,Zhuo Xunhui,Lu Shaohong
Background Diseases caused by Toxoplasma gondii ( T. gondii ) have introduced serious threats to public health . There is an urgent need to develop a rapid detection method for T. gondii infection in cats, which are definitive hosts. Recombinant apical membrane antigen 1 (rAMA1) was produced in a prokaryotic expression system and used as the detection antigen. The aim of this study was to evaluate and optimize a reliable indirect enzyme-linked immunosorbent assay (iELISA) method based on rAMA1 for the detection of antibodies against T. gondii in cats. Results The rAMA1-iELISA method was developed and optimized by the chessboard titration method. There were no cross-reactions between T. gondii -positive cat serum and positive serum for other pathogens, indicating that rAMA1-iELISA could only detect T. gondii in most cases. The lowest detection limit of rAMA1-iELISA was 1:3200 (dilution of positive serum), and the CV of repeated tests within batches and between batches were confirmed to be less than 10%. The results of 247 cat serum samples detected by rAMA1-iELISA (kappa value = 0.622, p < 0.001) were in substantial agreement with commercial ELISA. The ROC curve analysis revealed the higher overall check accuracy of rAMA1-iELISA (sensitivity = 91.7%, specificity = 93.6%, AUC = 0.956, 95% CI 0.905 to 1.000) than GRA7-based iELISA (sensitivity = 91.7%, specificity = 85.5%, AUC = 0.936, 95% CI 0.892 to 0.980). Moreover, the positive rate of rAMA1-iELISA (6.5%, 16/247) was higher than that of GRA7-based iELISA (3.6%, 9/247) and that of commercial ELISA kit (4.9%, 12/247). Conclusion The iELISA method with good specificity, sensitivity, and reproducibility was established and can be used for large-scale detection of T. gondii infection in clinical cat samples.
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-9
·
IF 5.479
Xue Jin-Hua,Liu Ling,Wang Yong-Sheng,Li Jia-Qian,Li Mei,Qu Yue-Ning,Li Le
This work describes a method for the determination of 1-hydroxypyrene (OH-Py) via aggregation-induced quenching of the emission of protamine-coated gold nanoclusters using 9-hydroxyphenanthrene (OH-Phe) as a sensitizer to boost the emission efficiency of nanoprobe. Under optimum conditions, the drop in fluorescence intensity at excitation/emission wavelengths of 300/596 nm is proportional to the concentrations of OH-Py in the range from 1.0 to 65 nM. The relative standard deviations are 4.2, 2.4 and 1.9% (for n = 11) at concentration levels of 8.0, 32 and 48 nM of OH-Py, respectively. The detection limit is 0.3 nM which is much lower than that of some previously reported methods. The recoveries from urine samples spiked with OH-Py ranged between 94.4 and 98.8%.
1-Hydroxypyrene (OH-Py) can trigger the aggregation of protamine-gold nanoclusters (PRT-AuNCs), resulting in the emission quenching of PRT-AuNCs. 9-Hydroxyphenanthrene (OH-Phe) can boost the emission efficiency of nanoprobe. Thereby, a highly sensitive assay of OH-Py was established.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
·
2024
·Vol. 43
·期 1
·1-22
·
IF 11.4
Tu Xiaoxuan,Chen Longxian,Zheng Yi,Mu Chenglin,Zhang Zhiwei,Wang Feiyu,Ren Yiqing,Duan Yingxin,Zhang Hangyu,Tong Zhou,Liu Lulu,Sun Xunqi,Zhao Peng,Wang Lie,Feng Xinhua,Fang Weijia,Liu Xia
Background The paucity of reliable biomarkers for predicting immunotherapy efficacy in patients with advanced hepatocellular carcinoma (HCC) has emerged as a burgeoning concern with the expanding use of immunotherapy. This study endeavors to delve into the potential peripheral biomarkers capable of prognosticating efficacy in HCC patients who are poised to receive anti-PD-1 monotherapy within the phase III clinical trial, KEYNOTE394. Additionally, we sought to elucidate the underlying molecular mechanisms for resistance to immune checkpoint blockade (ICB) and propose innovative combination immunotherapy strategies for future clinical application.Methods Patient blood samples were collected for single-cell RNA sequencing to evaluate the immune cell signature before receiving ICB therapy. Subsequently, in vitro assays and in vivo murine model experiments were conducted to validate the mechanism that S100A9+CD14+ monocytes play a role in ICB resistance.Results Our study demonstrates a notable enrichment of S100A9+CD14+ monocytes in the peripheral blood of patients exhibiting suboptimal responses to anti-PD-1 therapy. Moreover, we identified the Mono_S100A9 signature as a predictive biomarker, indicative of reduced efficacy in immunotherapy and decreased survival benefits across various tumor types. Mechanistically, S100A9 activates PD-L1 transcription by directly binding to the CD274 (PD-L1) gene promoter, thereby suppressing T-cell proliferation and cytotoxicity via the PD-1/PD-L1 axis, consequently diminishing the therapeutic effectiveness of subsequent anti-PD-1 treatments. Furthermore, our in vivo studies revealed that inhibiting S100A9 can synergistically enhance the efficacy of anti-PD-1 drugs in the eradication of hepatocellular carcinoma.Conclusion sOur study underscores the significance of S100A9+CD14+ monocytes in predicting inadequate response to ICB treatment and provides insights into the monocyte cell-intrinsic mechanisms of resistance to ICB therapy. We also propose a combined therapeutic approach to enhance ICB efficacy by targeting S100A9.
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2022
·Vol. 14
·期 9
·1680
·
IF 4.967
Qiuping He,Wei Chen,Pengfei Wang,Xiaoming Dou
Separation of dichlorobenzene (DCB) isomers with high purity by time− and energy−saving methods from their mixtures is still a great challenge in the fine chemical industry. Herein, silicalite-1 zeolites/polydimethylsiloxane (PDMS) hybrid membranes (silicalite-1/PDMS) have been successfully fabricated on the porous polyvinylidene fluoride (PVDF) supports to first investigate the pervaporation separation properties of DCB isomers. The morphology and structure of the silicalite-1 zeolites and the silicalite-1/PDMS/PVDF hybrid membranes were characterized by XRD, FTIR, SEM and BET. The results showed that the active silicalite-1/PDMS layers were dense and continuous without any longitudinal cracks and other defects with the silicalite-1 zeolites content no more than 10%. When the silicalite-1 zeolites content exceeded 10%, the surfaces of the active silicalite-1/PDMS layers became rougher, and silicalite-1 zeolites aggregated to form pile pores. The pervaporation experiments both in single-isomer and binary−isomer systems for the separation of DCB isomers was further carried out at 60 °C. The results showed that the silicalite-1/PDMS/PVDF hybrid membranes with 10% silicalite-1 zeolites content had better DCB selective separation performance than the silicalite-1/α−Al2O3membranes prepared by template method. The permeate fluxes of the DCB isomers increased in the order of m−DCB < o−DCB < p−DCB both in single-isomer and binary-isomers solutions for the silicalite-1/PDMS/PVDF hybrid membranes. The separation factor of the silicalite-1/PDMS/PVDF hybrid membranes for p/o−DCB was 2.9 and for p/m−DCB was 4.6 in binary system. The permeate fluxes of the silicalite-1/PDMS/PVDF hybrid membranes for p−DCB in p/o−DCB and p/m−DCB binary−isomers solutions were 126.2 g∙m−2∙h−1and 104.3 g∙m−2∙h−1, respectively. The thickness−normalized pervaporation separation index in p/o−DCB binary−isomers solutions was 4.20 μm∙kg∙m−2∙h−1and in p/m−DCB binary−isomers solutions was 6.57 μm∙kg∙m−2∙h−1. The results demonstrated that the silicalite-1/PDMS/PVDF hybrid membranes had great potential for pervaporation separation of DCB from their mixtures.
研究领域:
化学工程
·
材料科学
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DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 19
·11715-11721
·
IF 4.036
Baokang Zou,Yipeng Zhan,Xiuli Xie,TongTong Zhang,Runkai Qiu,Fangming Zhu
Two salalen titanium(IV) complexes ((H-salalen)TiCl2 and (F-salalen)TiCl2) containing hydrogen and fluorine respectively on the phenolate ring close to the imine were synthesized for the copolymerization of ethylene with 1-octene to prepare poly(ethylene-co-1-octene) in the presence of methylaluminoxane (MAO). The (F-salalen)TiCl2/MAO showed higher catalytic activity and better copolymer characteristics such as a higher molecular weight, narrower molecular weight distribution, and higher 1-octene incorporation than (H-salalen)TiCl2/MAO, which revealed that the electron-withdrawing conjugated effect introduced by fluorine substituents led to improvements on catalytic performance and thermal stability. The influences of copolymerization conditions including temperature, Al/Ti molar ratios and comonomer feed ratios on the copolymerization behavior of (F-salalen)TiCl2/MAO and the copolymer microstructure were investigated in detail. Under the activation of MAO, the (F-salalen)TiCl2 could produce ultrahigh molecular weight poly(ethylene-co-1-octene) with 1-octene incorporation ratios in the range of 0.9–3.1 mol% and exhibit relatively high activity. It could be inferred that long ethylene sequences in the copolymer were segregated by the isolated 1-octene units based on the 13C NMR characterization of the copolymer. Moreover, the thermal properties and crystallization of copolymers were determined by DSC and XRD and correlated to the ethylene sequence length distribution. The reactivity ratios calculated by the triad distribution in 13C NMR revealed the random comonomer distribution in the copolymer chain.
研究领域:
高分子化学
·
配位聚合
·
催化化学
·
材料科学
·
高分子物理
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DOI
高影响因子
LABORATORY INVESTIGATION
·
2019
·Vol. 100
·期 3
·378-386
·
IF 3.684
Lang Ping-Ping,Bai Jie,Zhang Yun-Long,Yang Xiao-Lei,Xia Yun-Long,Lin Qiu-Yue,Li Hui-Hua
Monocyte and adhesion infiltration into the arterial subendothelium are initial steps in hypertension development. The endothelial intercellular adhesion molecule-1 (ICAM-1) has been implicated in the recruitment and adhesion of leukocytes in several cardiac diseases. However, the role of ICAM-1 in angiotensin II (Ang II)-induced hypertension development remains unknown. Hypertension was induced by administering an infusion of Ang II (1000 ng/kg/min) to wild-type (WT) mice treated with an IgG control or ICAM-1 neutralizing antibody (1 and 2 mg/mouse/day, respectively). Blood pressure was determined using the tail-cuff system. Vascular remodeling was assessed by performing a histological examination. Inflammation and reactive oxygen species (ROS) levels were determined by using immunostaining. Vascular dysfunction was assessed by aortic ring assay. The expression of fibrotic markers, cytokines and NOX was evaluated by quantitative real-time PCR analysis. Our results demonstrate that Ang II infusion markedly increased the ICAM-1 level in the aorta. Blocking ICAM-1 with a neutralizing antibody significantly attenuated Ang II-induced arterial hypertension, vascular hypertrophy, fibrosis, macrophage infiltration, and ROS production and improved vascular relaxation. In conclusion, ICAM-1-mediated monocyte adhesion and migration play a critical role in Ang II-induced arterial hypertension and vascular dysfunction. ICAM-1 inhibitors may represent a new therapeutic strategy for the treatment of this disease.
研究领域:
心血管疾病
·
药理学
·
分子生物学
·
免疫学
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DOI
PubMed
高影响因子
PLoS One
·
2019
·Vol. 14
·期 2
·e0212335
·
IF 2.776
Yifan Zhang,Yong Chen,Yiting Cai,Zongyan Cui,Jinjie Zhang,Xiaohou Wang,Lirong Shen
Honey adulteration is becoming increasingly alarming incidents in food safety. Monitoring and detecting adulteration face greater challenges. Honey contains the major royal jelly proteins (MRJP) secreted by bee workers. To detect honey adulteration fast and accurately, a rapid gold sandwich immunochromatographic strip (GSIS) was developed based on two specific polyclonal antibodies (PoAbs) against the MRJP1, the most abundant protein of all MRJPs. We determined the best of pH value (pH 8.6) and PoAb SP-1 amount (5 μg/mL) in conjunction with colloidal. The cut-off value (sensitivity) of GSIS in detecting MRJP1 is 2.0 μg/mL in solution. Validation analysis with RJ, milk vetch honey, acacia honey and honey adulteration containing rice syrup and corn syrup with different ratios demonstrated that the GSIS could show consistent Test line (T line) when the test samples contain more than 30% pure honey or MRJP1 0.4 mg/g. The validation results by isotope ratio mass spectrometry on the same pure and all adulteration milk vetch honey samples showed the same information of GSIS test. The qualitative assay GSIS provided a valuable new way for honey authenticity and laid the foundation for the future application of GSIS with monoclonal antibodies in honey authentication.
研究领域:
食品科学
·
分析化学
·
农学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）