One Health Advances
·
2026
·Vol. 4
·期 1
·32
·
IF 5
Ni Baihai,Du Jiayu,Li Zimeng,Jian Pingan,Chen Mingshan,Li Jinlong,Li Xuenan
One common plasticizer is di(2-ethylhexyl) phthalate (DEHP). We use it a lot in our daily lives, and prolonged exposure to it can be harmful to living things. One form of selective autophagy that can break down damaged lysosomes is called lysophagy. Conversely, excessive occurrence of lysophagy can in turn cause lysosomal damage. Here, we show that DEHP stimulates the transient receptor potential mucoLipin 1-transcription factor EB (TRPML1-TFEB) pathway, drives excessive lysophagy, resulting in lysosomal dysfunction and kidney injury in mice. One carotenoid with strong antioxidant qualities is lycopene (LYC). It is frequently employed to offset the effects of DEHP. Interestingly, our results showed that LYC can reduce kidney damage caused by DEHP in mice. Inhibiting the TRPML1-TFEB pathway's activation and reducing lysophagy are two of the mechanisms of action. In summary, LYC significantly alleviates DEHP-induced injury in mouse renal tubular epithelial cells by inhibiting TRPML1-TFEB pathway-mediated lysophagy. LYC successfully reduced the damage caused by mono(2-ethylhexyl) phthalate in human renal proximal tubular epithelial cells, confirming this effect in vitro. This study establishes a critical link between LYC treatment and lysophagy as well as lysosomal dysfunction in DEHP-induced mouse renal tubular epithelial cells. This study offers novel mechanistic perspectives on DEHP-induced nephrotoxicity and the renal protection conferred by LYC. Graphical
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2022
·
IF 3.119
Yingmin Yu,Xiaocheng Zhang,Lingxiao Yi,Min Li,Fangyu Zhang,Jiajun Wei
Liquid–liquid equilibrium (LLE) data of ternary systems {2-ethyl-1-hexanol + ethylene glycol + water/n-heptane} were measured at 298.2, 308.2, and 318.2 K under 101.3 kPa. The distribution coefficient (D) and selectivity coefficient (S) of the systems were calculated to analyze the extraction ability of the two extractants that separate 2-ethyl-1-hexanol and ethylene glycol. Meanwhile, the NRTL and UNIQUAC activity coefficient models were used to correlate the experimental data and the different model parameters were obtained by data regression. The root-mean-square deviation (RMSD) between the calculated and experimental values was less than 0.96%, indicating that both models have a reasonable correlation with the experimental data of the ternary system and can accurately predict the experimental data. GUI-MATLAB has validated the binary interaction parameters.
研究领域:
化学工程
·
热力学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yani Liu,Xueao Wang,Zheng Li,Liping Chen,Xinyi Cai,Zhigang Sun,Wei Cheng,Xiangjie Luo,Hai-Liang Zhu,Yong Qian
Hydrogen sulfide (H2S), a critical gas signaling molecule, and N-acetyltransferase 2 (NAT2), a key enzyme in drug metabolism, are both known active biomarkers for liver function. However, the interactions and effects of H2S and NAT2 in living cells or lesion sites remain unknown due to the lack of imaging tools to achieve simultaneous detection of these two substances, making it challenging to implement real-time imaging and precise tracking. Herein, we report an activity-based two-photon fluorescent probe, TPSP-1, for the cascade detection of H2S and NAT2 in living liver cells. Continuous conversion from TPSP-1 to TPSP-3 was achieved in liver cells and tissues. Significantly, leveraging the outstanding optical properties of this two-photon fluorescent probe, TPSP-1, has been effectively used to identify pathological tissue samples directly from clinical liver cancer patients. This work provides us with this novel sensing and two-photon imaging probe, which can be used as a powerful tool to study the physiological functions of H2S and NAT2 and will help facilitate rapid and accurate diagnosis and therapeutic evaluation of hepatocellular carcinoma.
研究领域:
生物化学
·
医学影像学
·
肿瘤学
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DOI
PubMed
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2021
·
IF 4.354
Qingqiang Tian,Shasha Xu,Chiying Zhang,Xinyi Liu,Xiangwei Wu,Yahui Li
Herein, an interesting palladium-catalyzed procedure for the direct carbonylative thiomethylation of aromatic amine derivatives with 4-methylthio-2-butanone is developed. Using 4-methylthio-2-butanone as (methylthio) transfer agent, a variety of corresponding thioesters are obtained with moderate to good yields under base-free condition. In addition, good functional group tolerance can be observed.
研究领域:
有机化学
·
合成化学
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DOI
PubMed
高影响因子
Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy
·
2024
·
IF 1.4
Xie Huilong,Fu Yunrong,Lin Zhisheng,Wang Jiayi,Wu Jingyu,You Xiuhua,Xie Jieming
This study explores the synergistic effect of T-CUS 245C combined with cyclosporin A, and its possible mechanism. T-CUS 245C is an immunotoxin conjugated with tastuzumab and recombinant cucurmosin 245C (CUS 245C ). Cyclosporin A is a calcium antagonist mainly used as an immune-suppressive agent, which enhances the cytotoxicity of immunotoxins through an unknown mechanism. The sulforhodamine B assay, flow cytometry, DNA fragmentation apoptosis assay, and western blot analysis were used to evaluate the synergistic effects of cyclosporin A combined with T-CUS 245C on proliferation inhibition and apoptosis induction in SK-OV-3 ovarian cancer cells with HER-2-overexpressing. Meanwhile, using confocal microscopy and flow cytometry, the effect of cyclosporin A on the intracellular distribution of T-CUS 245C was observed and quantified. The results of this study showed that the combination index of cyclosporin A combined with T-CUS 245C is less than 0.7, indicating that cyclosporin A significantly enhances the proliferation inhibition and apoptosis induction induced by T-CUS 245C in SK-OV-3 cells. The confocal microscopy and flow cytometry results show that cyclosporin A effectively increases the dispersion of T-CUS 245C in the cytoplasm by promoting the endolysosomal escape. This study demonstrates that cyclosporin A combined with T-CUS 245C has a synergistic anticancer effect, by facilitating the release of T-CUS 245C in the intracellular space. Graphical Abstract
研究领域:
肿瘤学
·
细胞生物学
·
药理学
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DOI
高影响因子
MATERIALS RESEARCH BULLETIN
·
2021
·
IF 4.641
Jiayu Li,Jiqing Lv,Feng Shi,Ze-Ming Qi,Runhao Han,Jing Wang
Double perovskite structured Ba(Mg 1/2 Mo 1/2 )O 3 (BMM) ceramics were prepared by traditional solid sintering methods at different sintering temperatures (T s =1250–1325 °C). X-ray diffraction (XRD) data proved a 1:1 ordered face-centered cubic lattice (Fm-3 m), and the ceramic sintered at 1300 °C obtained maximum relative density. The lattice vibration characteristics were identified by Raman spectroscopy and far-infrared reflection spectroscopy. Based on the far-infrared data, a four-parameter semi-quantum model (FPSQ) was applied to calculate the intrinsic properties of BMM ceramics, and the obtained results approximated the measured values. The Raman shift of the BMM F 2g (B) mode (attributed to the centrosymmetric vibration of Ba atom) was shown to be negatively correlated with the corrected permittivity and Ba-O bond length. With increasing sintering temperature (T s ), the relative density is positively correlated with the quality factor ( Q × f) , and the change of Ba-O bond length leads to distortion of the oxygen octahedron, which is positively correlated with the dielectric constant. The sintered BMM ceramics obtained their best dielectric properties at 1300 °C ( ε r = 4.987, Q × f = 23,069 GHz, f = 11.16 GHz).
研究领域:
材料科学
·
物理学
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DOI
高影响因子
Journal of Energy Storage
·
2023
·
IF 9.4
Yu-Qin Mao,Guang-He Dong,Yuan-Qing Li,Pei Huang,Shao-Yun Fu
Doping engineering is a high-efficiency strategy to introduce heteroatom species and modify the surface electronic structures of porous carbon materials. However, the conventional doping strategies for porous carbons usually involve with either complicated fabrication processes or difficulty in quality control. Herein, nitrogen‑oxygen self-doped porous carbons (NOPCs) with adjustable structure are prepared by a facile one-step process of direct carbonization/activation the mixture of 2-methylimidazole (2-MeIM) and KOH. The as-prepared NOPCs possess hierarchical porous structure with interconnected micro-/ meso -/macropores, high specific surface area (2900 m 2 g −1 ), large pore volume (1.2 cm 3 g −1 ) and high N,O heteroatoms level (26.8 at.% in total), which exhibit ultra-high specific capacitance of 424.2 F g −1 at 1.0 A g −1 in a three-electrode system in 2 M KOH electrolyte and 173.4 F g −1 at 1.0 A g −1 in a two-electrode system in EMIMBF 4 ionic liquid electrolyte. Impressively, the symmetric supercapacitor based on NOPC exhibits a wide operating voltage window up to 3 V, a high energy density of 56.4 Wh kg −1 at a power density of 373.6 W kg −1 , excellent rate performance with the retention of ~76.2 % from 1.0 to 100 A g −1 , and splendid cycling stability with only 0.0011 % capacity fading per cycle within 10,000 loops. Consequently, the one-step carbonization/activation strategy with commercial-available 2-MeIM as raw material is promising for mass production of high performance NOPC materials.
研究领域:
材料科学
·
能源存储与转换
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DOI
高影响因子
MINERALS ENGINEERING
·
2018
·
IF 2.707
Wencui Chai,Yanfang Huang,Weijun Peng,Guihong Han,Yijun Cao,Jiongtian Liu
The existence of sulfide minerals (mainly pyrite) limits the use of high-sulfur bauxite in the production of Bayer alumina. In this work, the reverse flotation desulfurization using 2-mercaptobenzimidazole (MBI) as the collector was studied by L 9 (3 4 ) orthogonal tests. The interaction mechanisms of MBI with pyrite were investigated by advanced analysis technologies including scanning electron microscopy, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), contact angle testing and zeta potential analysis. Results indicate that the sulfur content of bauxite is decreased from 2.87% to 0.26% by one roughing and one cleaning flotation under the optimized conditions of collector dosage of 100 g/t, activator dosage of 60 g/t, frother dosage of 200 g/t and pulp pH at 3. MBI exhibits superior hydrophobic behavior on the pyrite surface under the acid solution than under the alkaline solution, which favors the flotation of pyrite under the acidic condition. The zeta potential, FTIR and XPS results reveal that the interaction mechanisms of MBI with pyrite are electrostatic forces and chelation by forming N Fe S coordination bonds. MBI is an efficient collector of pyrite in the flotation desulfurization of high-sulfur bauxite and is expected to be used in other oxide or sulfide ores to separate pyrite.
研究领域:
矿物加工工程
·
冶金工程
·
表面化学
·
资源综合利用
·
浮选化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Qiang Chu,Meng Chen,Dongxiao Song,Xixi Li,Yunyun Yang,Zihuan Zheng,Yonglu Li,Yangyang Liu,Lushuang Yu,Zheng Hua,Xiaodong Zheng
Oxidative stress is a critical cause for the progression of neurodegenerative diseases, indicating that screening natural antioxidant and pharmacological targeting of mechanism need more attention. Natural plant polysaccharides are the primary group of natural antioxidants showing mighty antioxidant activity. Therefore, a polysaccharide (AFP-2) from flowers of Apios americana Medik was isolated, purified and characterized. Then, a H 2 O 2 -induced PC12 cells oxidative stress model was established to investigate the neuroprotective role of AFP-2 and to clarify the potential mechanism of action against H 2 O 2 -induced neurotoxicity. It was found that AFP-2 reduced ROS production and mitochondrial damage caused by hydrogen peroxide in PC12 cells. In addition, data from transmission electron microscope and western blot displayed that AFP-2 significantly activated autophagy in PC12 cells via Akt-mTOR pathway. These results together qualify AFP-2 as an interesting natural polysaccharide worth further investigation as a neuroprotective agent.
研究领域:
神经科学
·
药理学
·
分子生物学
·
天然产物化学
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DOI
PubMed
高影响因子
JOURNAL OF NON-CRYSTALLINE SOLIDS
·
2023
·
IF 3.5
Yichen Ye,Yongbao Xiao,Yao Ji,Weichao Wang
Singly Ho 3+ -doped TeO 2 –Ta 2 O 5 –ZnO (TTZ) glass and optical fiber for 2 μm fiber lasers have been investigated. The glass-forming region of the ternary TTZ glass system is determined to study its glass-forming ability and to select an appropriate glass matrix for doping Ho 3+ ions. Upon the excitation of a 1940 nm laser diode (LD), an intense emission at 2.06 μm with a full width at half maximum (FWHM) of 61 nm is observed from the Ho 3+ -doped TTZ glass. The measured lifetime of the Ho 3+ : 5 I 7 level in TTZ glass shows a gradual decrease from 3.5 to 0.98 ms as the dopant concentrations increase from 0.2 to 2 mol%. A large emission cross-section (σ e MC ∼ 4.56 × 10 −21 cm 2 ) and figure of merit (FOM, ∼ 6.83 × 10 −24 cm 2 ·s) are obtained. The Ho 3+ -doped TTZ glass is further fabricated into an optical fiber with an average transmission loss of 0.12 dB/cm at 1310 nm. The results show that singly Ho 3+ -doped TTZ glass and optical fiber are promising candidates for efficient 2 μm fiber lasers.
研究领域:
光学
·
材料科学
·
光子学
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DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2022
·
IF 4.171
Xiaoya Bu,Yan-gai Liu,Jian Chen
A series of original tunable-luminescence phosphors K 3 Lu(PO 4 ) 2 : Tb 3+ , Eu 3+ with efficient energy transfer (ET) was synthesized using a high-temperature solid-state reaction. The samples were characterized via X-ray powder diffraction, X-ray photoelectron spectroscopy, photoluminescence spectroscopy, CIE chromaticity coordinates, and fluorescence decay analysis. The energy band and density of states of K 3 Lu(PO 4 ) 2 were analyzed by the first-principles method. The cross-relaxation of Tb 3+ -doped phosphors was investigated as well. The ET from Tb 3+ to Eu 3+ ions, realizing through the electric dipole-dipole (d-d) interaction with a high ET efficiency of 98.36%, could be controlled principally by the Tb 3+ /Eu 3+ doping concentration ratio, yielding the tunable emissions from green (0.293, 0.537) to orange-red (0.563, 0.367) range. Based on the obtained results, a three-phosphor-conversion white light-emitting diode (w-LED) was fabricated through the integration of a 365 nm chip and commercial blue-emitting BaMgAl 10 O 17 : Eu 2+ phosphors, green-emitting SiAlON: Eu 2+ phosphors, and orange-red-emitting K 3 Lu(PO 4 ) 2 : 0.1 Tb 3+ , 0.06Eu 3+ phosphors. The appliance emits the white light at a suitable correlated color temperature of 3678 K and a high color rendering index of 91.4. These parameters are almost equal to those of ideal white light, which opens up the prospects for using this up-and-coming phosphor for warm w-LED applications.
研究领域:
材料科学
·
光学
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DOI
高影响因子
EXPERIMENTAL NEUROLOGY
·
2025
·
IF 4.2
Jun Fu,Chengjie Ding,Wenqing Li,Jiali Lei,He Wang,Lingfei Meng,Xiaoya Lin,Weiyuan Chen,Hongchang Gao
Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive cognitive decline, with hallmark pathological features including Aβ deposition and tau protein hyperphosphorylation. These pathological alterations are particularly prominent in the Prefrontal Cortex (PFC) and exert profound effects on cognitive function. The present study aimed to reveal key proteins in the PFC and provide insight into their potential mechanisms of action in AD. Accordingly, proteomic analysis was conducted to profile differential proteins in 5 × FAD mice, followed by in vivo and in vitro validation of core proteins through AAV-mediated interference of target gene expression. Furthermore, transcriptomics and molecular biology techniques were employed to confirm the underlying mechanisms of action of core proteins in AD. The results demonstrated that ELAVL1 was significantly upregulated in the PFC of AD mice. Behavioral experiments revealed that inhibition of ELAVL1 expression markedly improved long-term memory in AD mice. Immunostaining and Western blot analyses confirmed that suppressing ELAVL1 expression alleviated Aβ1–42 deposition and apoptosis. In vitro experiments indicated that knocking down ELAVL1 significantly reduced the apoptotic response of SH-SY5Y cells induced by Aβ1–42 and APP/Swe cells. Transcriptomic analysis further revealed that ELAVL1 regulates Aβ1–42-induced apoptosis via activation of the Bcl-2/Bax pathway. Notably, immunofluorescence co-localization staining and pull-down assays confirmed that ELAVL1 directly interacts with APP. Collectively, these findings provide valuable insights into the molecular mechanisms underlying AD and suggest ELAVL1 as a promising therapeutic target for AD.
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DOI
PubMed
高影响因子
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