JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2017
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IF 3.012
Yinxiu Zuo,Jingkun Xu,Fengxing Jiang,Xuemin Duan,Limin Lu,Guo Ye,Changcun Li,Yongfang Yu
Gold nanoparticles (AuNPs) dotted S-doped carbon (AuNPs/S-C) nanoflakes were synthesized via a facial and simple approach. Typical synthesis route involves the formation of gold nanoparticles/poly(3,4-ethylenedioxythiophene) (AuNPs/PEDOT) and a subsequent calcination process. Many techniques, including scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) were used to characterize their morphology, structures and compositions . Results indicated that AuNPs with an average size of 5 nm were uniformly dotted on/into the S-C nanoflakes. The AuNPs/S-C/nafion was utilized as a new electrode material for simultaneously electrochemical determination of Cu (II) and Hg (II) using differential pulse anodic stripping voltammetry (DPASV) technique. Benefiting from the efficient metal-complexing ability and electrocatalytic activity of S-C and the good conductivity of AuNPs, the modified electrode exhibited enhanced electrochemical performance compared with bare glassy carbon electrode. Under optimal conditions, the peak currents displayed linear response to the concentrations of Cu (II) and Hg (II) ranges from 0.64 to 63.55 μg/L and 4.01 to 300.89 μg/L, with detection limits of 0.19 μg/L and 1.40 μg/L (S/N = 3), respectively. The modified electrode was also applied for the determination of Cu (II) and Hg (II) in lake water samples with good results.
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Food Chemistry-X
·
2024
·
IF 6.5
Hua Zheng,Linlin Feng,Zheng Huang,Ziwei Zou,Xiaolong Ma,Ziping Pan,Jinfeng Li,Jinxia Wu,Mei Li,Zhiheng Su
Aflatoxin B1 (AFB1) is a potent toxin in food, necessitating rapid, instant, and sensitive detection. We have engineered an electrochemical sensor to monitor AFB1 using a system composed of Fe 3 O 4 -NH 4 /AuNPs/apt-S1. The aptamer specifically recognizes AFB1, while ‘S1’ is functionalized with methylene blue to enhance the current. The RecJf exonuclease promotes the formation of the electrochemical strategy. The Fe 3 O 4 component, with its magnet properties, enables a rapid separation of solids and liquids without the need for instrumentation. The sensor exhibits a linear range for AFB1 ranging from 1 ng to 10 μg. The regression equation is I(nA) = 446.8 × logc+2085 (where I and c represent the peak current and AFB1 concentration, respectively). The correlation coefficient is 0.9508, and the detection limit is 3.447 nM. The relative standard deviation of AFB1 in peanut oil ranges from 4.80% to 6.80%. These results demonstrate that the sensor has high sensitivity, stability, repeatability, and specificity for AFB1 detection.
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yan Liu,Yuetao Zhang,Zijie Li,Zhiqi Li,Shaoke Zhou,Yong Xia,Lei Mou
Rapid, accurate, and cost-effective strategies for the multiplexed and quantitative detection of multiple infectious disease biomarkers are still lack in hospital. Here, we introduce a multiplexed screen-printed electrode for the simultaneous detection of three infectious disease biomarkers: HBsAg, anti-HIV, and anti-TP. The electrode modified with in-situ deposition of gold nanoparticles (AuNPs) and MXene multilayered nanosheets. Comprehensive characterization confirms the morphology and electrochemical response of the AuNPs&MXene modified screen-printed electrode (AuNPs&MXene-SPE). AuNPs&MXene composite contributes to enhance sensitivity by maximizing surface area and improving electron transfer efficiency. The AuNPs&MXene-SPE exhibits outstanding detection performance for HBsAg, anti-HIV, and anti-TP. The AuNPs&MXene-SPE sensors achieve broad detection ranges (0.05–1000 ng/mL for HBsAg, 0.35–140 ng/mL for anti-HIV, and 0.25–100 ng/mL for anti-TP) accompanied by low limits of detection (0.01, 0.11, and 0.10 ng/mL, respectively). Applied to standard serum samples, the sensors showcase precision with relative standard deviations consistently below 5.0 %, affirming their suitability for complex solution environments. These sensors offer notable advantages, including cost-effectiveness, rapid response, high sensitivity, and specificity, eliminating the necessity for expensive equipment, which open avenues for the realization of point-of-care testing (POCT).
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Shanshan Wang,Xu Hun,Xiliang Luo
The continued expansion of the fields of photoelectrochemical (PEC) aptasensor has guided the development of photoactive materials that can generate electron-photo pairs to emerge photocurrent. However, only such an improvement has an insatiable appetite for the application of molecular level for PEC aptasensor due to the limited quantity of the bioprobes immobilized on the working electrode. Here, we successfully synthetized SnSe/graphene (SnSe/GR) nanohybrids as a photoactive material with a strong photocurrent signal. The constructed bioprobe, DNA/gold nanoparticles/glutathione disulfide (DNA/AuNPs/GSSG), decreased photocurrent effectively. GSSG, an electron acceptor, was designed as a quencher in the bioprobe to overcome a part of shortcomings of DNA/AuNPs in a way. This was owing to the resonance energy transfer (RET) between AuNPs and SnSe/GR, and GSSG to accept photoelectrons, so that the photocurrent response was obviously decreased comparing to the PEC response of SnSe/GR/CPE. Based on this, probe/SnSe/GR/CPE was built as a signal-on PEC aptasensor to detect endocrine-disrupting chemicals using bisphenol A as model. This signal-on PEC aptasensor showed a linear response in the range of 0.01–7 μM and a limit of detection of 3 nM for bisphenol A. And this method presented a promising prospect for sensitive and accurate analysis of other biological molecules between aptamers and corresponding targets at low levels.
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Journal of Environmental Chemical Engineering
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2024
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IF 7.4
Meng Fu,Chang Luo,Qi Yuan,Zequn Ma,Quansheng Long,Baihui Liu,Dang Wu,Jingshui Xu,Xiangming Li
The development of bacterial resistance to antibiotics and its global spread highlights the urgent need for new alternative antimicrobial agents. Here, we have developed a biosafe antibacterial material based on reduced graphene oxide/polyelectrolytes nanolayers and gold nanoparticles functionalized molybdenum disulfide (rGP 1.5 /MoS 2 -AuNPs) via in situ synthesis and electrostatic assembly. The rGP 1.5 /MoS 2 -AuNPs has high visible and near-infrared absorption, which can efficiently provide remarkable photothermal conversion efficiency and generate reactive oxygen species (·O 2 - , ·OH, 1 O 2 ) via photocatalytic oxygen reduction and water oxidation. The rGP 1.5 /MoS 2 -AuNPs combines the photocatalysis with photothermal effect, exhibiting a biosafe and effective killing for Gram-negative and Gram-positive bacterial. The rGP 1.5 /MoS 2 -AuNPs simultaneously optimizes three key parts for antibacterial: i) polyelectrolytes component promotes bacterial adhesion, ii) reduced graphene oxide and AuNPs components provide photothermal conversion capacity and increase light absorption, iii) MoS 2 component generates photogenerated electron-hole pairs, and the electrons are rapidly conducted from the AuNPs to the reactants, which promotes the rapid separation of the electron-hole pairs, resulting in the generation of reactive oxygen species. This work provides a simple but powerful way to prepare photocatalytic and photothermal material for antibacterial applications.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 2
·1-11
·
IF 5.833
Wang Xiaoying,Li Linyu,Gu Xuan,Yu Bingjia,Jiang Meng
The aggregation of amyloid-β oligomers (AβOs) with extremely strong neurotoxicity has been proved to be the main pathogenesis of Alzheimer’s disease (AD). For sensitive quantification of AβOs, a switchable electrochemical aptasensor is proposed. Metal organic framework carrying Au nanoparticles (AuNPs@CuMOF) has been used to label signaling displaced-probe (SD), which formed triple helix switch (THS) by hybridizing with label-free anti-AβOs aptamer (Apt) on the electrodeposited palladium electrode (EPd). Thus, a relatively strong response of differential pulse voltammetry (DPV) was produced (switch on). With the specific binding between AβOs and Apt, the DPV response obviously decreased, owing to destroyed structure of THS and the separation of AuNPs@CuMOF/SD from the EPd (switch off). The mode of “switch on-off” can dramatically enhance the AβOs-dependent DPV intensity change. As a result, the switchable EA exhibited excellent selectivity and sensitivity with the linear range from 0.5 fM to 500 fM and the detection limit of 0.25 fM. When evaluating the AβOs of artificial cerebrospinal fluid (aCSF) samples, the switchable EA exhibited desirable feasibility, and the results are basically consistent with the enzyme linked immunosorbent assay (ELISA). The work could provide a potential tool of the AD diagnosis and a bright future in clinical applications.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
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IF 7.7
Zepeng Gu,Xiaoyao Wang,Qinyan Xu,Xianfeng Lin,Nuo Duan,Shijia Wu
Due to signal suppression caused by large interfering macromolecules such as proteins, reliable and label-free detection of antibiotics in milk remains a significant challenge. Here, a surface-enhanced Raman scattering (SERS) platform based on self-assembled molecular layers combined with deep learning was developed to selectively enrich and enhance representative antibiotics. A gold nanoparticle monolayer SERS substrate with an average gap of 2.5 nm was successfully fabricated, achieving a detection limit as low as 4.88×10 -10 M for 4-MBA. Notably, this technique effectively suppressed the Raman signals from macromolecules in milk while allowing antibiotic molecules to access the nanogap hotspot regions. The detection limits for penicillin and ciprofloxacin in milk were determined to be 8.4×10⁻ 9 M and 5.34×10 -9 M, respectively. Additionally, to address the issue of spectral overlap caused by the simultaneous presence of multiple antibiotics, which traditional chemometric methods cannot resolve for accurate quantification, a novel deep learning model, RamEfficientNet, was developed. Unlike conventional single-peak approaches, this model analyzes the entire spectral range through CBAM attention mechanisms, enabling extraction and decomposition of individual antibiotic spectra from complex mixtures for precise quantification. The model successfully achieved rapid classification of Raman spectra for various concentrations and types of antibiotics, with a classification accuracy exceeding 95%. Furthermore, by combining this model with an MLP, an innovative artificial intelligence visualization of the spectral regression process was realized, with an R² value exceeding 0.99. In conclusion, the combination of gold nanoparticle gaps and artificial intelligence provides a solution for the simultaneous quantitative analysis of antibiotics.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2024
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IF 3.1
Wendi Mu,Chunyan Wu,Fangfang Wu,Hongmin Gao,Xinshui Ren,Jing Feng,Meng Miao,Hehua Zhang,Dong Chang,Hongzhi Pan
Cancer antigen 125 (CA125) is pivotal as a tumor marker in early ovarian cancer prevention and diagnosis. In this work, we introduced an ultrasensitive label-free electrochemical immunosensor tailored for CA125 detection, leveraging nanogold-functionalized copper-cobalt oxide nanosheets (CuCo-ONSs@AuNPs) as nanocomposites. For the inaugural application, copper-cobalt oxide nanosheets delivered the requisite DPV electrochemical response for the immunosensors. Their large specific surface area and commendable electrical conductivity amplify electron transfer and enable significant gold nanoparticle loading. Concurrently, AuNPs offer a plethora of active sites, facilitating easy immobilization of biomolecules via the bond between amino groups and AuNPs. We employed scanning electron microscopy, transmission electron microscopy, and x-ray photoelectron spectroscopy to characterize the nanomaterials' surface morphology and elemental composition. The electrochemical sensor response signals were ascertained using differential pulse voltammetry. Under optimal conditions, the immunosensor exhibited a linear detection range from 1×10 −7 U/mL to 1×10 −3 U/mL and a detection limit of 3.9×10 −8 U/mL (S/N=3). The proposed label-free electrochemical immunosensor furnishes a straightforward, dependable, and sensitive approach for CA125 quantification and stands as a promising method for clinical detection of other tumor markers.
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FOOD CONTROL
·
2026
·
IF 7
Mengdie Luo,Wenpei Xie,Jingjing Fan,Xiaojun Chen,Gang Peng
Lead ion (Pb 2+ ) contamination poses severe threats to food safety and human health, demanding rapid, sensitive, and reliable on-site analytical detection techniques. Herein, a colorimetric-electrochemical dual-mode aptasensor was developed for the ultrasensitive detection of Pb 2+ in rice, utilizing a dual-signal amplification strategy integrating bismuth terephthalate material (BiBDC)-AuNPs nanozyme and RecJf exonuclease (RecJf Exo)-assisted target recycling. BiBDC uniformly disperses and immobilizes AuNPs, significantly enhancing their peroxidase-like catalytic activity for robust signal output. Meanwhile, RecJf Exo specifically hydrolyzes Pb 2+ -bound aptamer strands to achieve cyclic release and reuse of target Pb 2+ , effectively amplifying the response signal. Under optimal conditions, the dual-mode aptasensor exhibited low detection limits of 4.52 pg/mL (colorimetric) and 0.035 pg/mL (electrochemical). The developed sensor demonstrated excellent specificity, reproducibility, stability, and satisfactory recoveries (93.54–102%) in real rice samples. This dual-signal amplification strategy provides a reliable platform for highly sensitive and accurate detection of Pb 2+ in food matrices, showing great potential for food safety monitoring.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Mengdie Luo,Jingjing Fan,Wenpei Xie,Gang Peng,Xiaojun Chen
The severe health risks associated with cadmium-contaminated food necessitate the development of accurate and rapid detection technologies. Herein, a novel pH-switchable multi-enzyme activity Bi 12 Co 0.6 O 18.8 -AuNPs is designed to construct a dual-mode aptasensor for the rapid and reliable detection of Cd 2+ . This nanozyme exhibits pH-modulated catalytic behaviors, demonstrating peroxidase-like activity under acidic conditions (pH 4) to catalyse the H 2 O 2 -mediated oxidation of 3,3′,5,5′-tetramethylbenzidine for visual colorimetric detection, while switching to catalase-like activity at slightly alkaline conditions (pH 8) to decompose H 2 O 2 into O 2 , thereby generating a measurable electrochemical signal. Benefiting from the collaborative enhancement mechanism of the dual-mode strategy through cross-validation and the high specificity of aptamer recognition, the sensor achieves highly precise and reliable detection of Cd 2+ . The sensor shows ultra-sensitive detection with limits of detection of 9.23 pg/mL (colorimetric) and 0.43 pg/mL (electrochemical). Its successful application in real rice samples confirms its practical utility for food safety monitoring.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Qian Zeng,Peng Liu,Yihui Chen,Yingnan Li,Hongyu Xiao,Li Qingwei,Anna Li,Jingye Liang,Weiqiang Li,Yangping Wen,Kaijie Tang
A Bi-rich BiOBr/BC@AuNP nanocomposite was developed as an efficient electrochemical sensing platform. • ML − assisted central composite design enabled precise optimization of MIP conditions. • MIP electrochemical sensor for trace detection of sulfachlorpyrazine sodium in food. A highly sensitive and selective molecularly imprinted electrochemical sensor was developed for the detection of sulfachloropyrazine sodium (SPZ), assisted by machine learening (ML) optimization. The sensing platform was fabricated using novel Bi-rich BiOBr/BC@AuNPs nanocomposite, where the synergistic combination of Bi-rich BiOBr, conductive biochar, and gold nanoparticles markedly enhanced electron transfer and provided abundant electroactive sites. Critical imprinting parameters, including the functional monomer-to-template ratio, electropolymerization cycles, elution time, and incubation time, were optimized using a central composite design coupled with ML algorithms like support vector regression. Under optimal conditions, the sensor displayed a wide linear range of 0.031–61.34 ng/mL, with a low detection limit of 0.0025 ng/mL and a quantification limit of 0.0077 ng/mL. The sensor exhibited excellent selectivity, effectively distinguishing SPZ from structurally analogous sulfonamide. Successful application to spiked chicken, milk, and honey samples yielded satisfactory recoveries and reproducibility, confirming its strong potential for practical food safety.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2022
·
IF 3.428
Jiangdong Gu,Qiufeng An,Jiamin Chen,Yafei He,Wenhuan Huang
To detect acetaminophen (AMP) and dopamine (DA) effectively and rapidly, the Au nanoparticles (AuNPs） were deposited on the surface of glassy carbon electrode (GCE) modified with Molybdenum dioxide-doped Reduced graphene oxide (RGO-MoO 2 ) nanosheets to form AuNPs/RGO-MoO 2 /GCE composite modified electrode (electrochemical sensor) was prepared The modified electrode was studied regarding its composition, structure and the effect of process parameters such as the electrochemical response characteristics of AMP and DA, the pH value of the buffer, the scan rate, and the sensitivity of both simultaneous detections. The results show that the synergy between AuNPs and RGO-MoO 2 nanomaterials can promote the electrochemical reaction of AMP and DA, and the oxidation peak potential differences of the two are greater than 180 mV. The linear ranges of the modified electrode are 0.05–320 μ mol/L for AMP and 0.6–260 μ mol/L for DA, and the detection limits are 7.2 nmol/L and 13 nmol/L (S/N = 3), respectively. The prepared AuNPs/MoO 2 -RGO/GCE modified electrode has a wider linear range, lower detection limit, excellent reproducibility, stability and selectivity.
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