ANALYTICAL BIOCHEMISTRY
·
2016
·
IF 2.243
Shijia Wu,Nuo Duan,Weixiao Zhang,Sen Zhao,Zhouping Wang
Patulin (PAT) is a kind of mycotoxin that has serious harmful impacts on both food quality and human health. A high-affinity ssDNA aptamer that specifically binds to patulin was generated using systemic evolution of ligands by exponential enrichment (SELEX) assisted by graphene oxide (GO). After 15 rounds of positive and negative selection, a highly enriched ssDNA pool was sequenced and the representative sequences were subjected to binding assays to evaluate their affinity and specificity. Of the eight aptamer candidates tested, the sequence PAT-11 bound to patulin with high affinity and excellent selectivity with a dissociation constant ( K d ) of 21.83 ± 5.022 nM. The selected aptamer, PAT-11, was subsequently used as a recognition element to develop a detection method for patulin based on an enzyme-chromogenic substrate system. The colorimetric aptasensor exhibited a linear range from 50 to 2500 pg mL −1 , and the limit of detection was found to be 48 pg mL −1 . The results indicated that GO-SELEX technology was appropriate for the screening of aptamers against small-molecule toxins, offering a promising application for aptamer-based biosensors.
研究领域:
毒理学
·
分析化学
·
食品科学
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DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Zixuan Shang,Pengfei Ma,Imran Mahmood Khan,Ningru Yang,Zhouping Wang
Currently, the technology for detecting antibiotics is developing rapidly, with good sensitivity and selectivity. However, some detection methods still have issues with cumbersome pretreatment and high experimental costs. Herein, A “turn-off” bifunctional DNA tweezer was constructed through the design of DNA sequence and self-assembly technology for the dual detection of Enrofloxacin (ENR) and Kanamycin (KANA) in a food matrix. The operation is simple, which increases the detection speed and reduces the detection cost. The two ends of the bifunctional DNA tweezers are connected with specific split aptamer sequences, and the split aptamer forms a specific ternary complex with the target. The tweezers “clip” ENR and KANA at their respective ends, causing a structural change. Under the optimal experimental conditions, the linear range of detection ENR is 0.2 ng/mL-1000 ng/mL with an LOD of 0.18 ng/mL, the linear range of detection KANA is 0.5 ng/mL-1000 ng/mL with an LOD of 0.36 ng/mL. The bifunctional DNA tweezers involved provide a new idea for the construction of DNA nanomachines, which is also suitable for the recognition and detection of other small molecules.
研究领域:
生物化学
·
食品科学
·
纳米技术
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2011
·Vol. 21
·期 38
·14639-14645
·
IF 5.101
Xiao-Jun Cai,Hai-Qing Dong,Wen-Juan Xia,Hui-Yun Wen,Xue-Quan Li,Jin-Hai Yu,Yong-Yong Li,Dong-Lu Shi
Engineered PEG-detachable catiomers were developed as non-viral gene vectors to detach the PEG layers responsive to the intracellular reducing environment. These catiomers were found to exhibit high DNA binding ability and reduced cytotoxicity, as evidenced in agarose gel electrophoresis and MTT assays. The size of the mPEG-SS-PLL/DNA complexes was around 100 nm with a regular spherical shape, as observed under transmission electron microscopy (TEM). The complexes were stably dispersed in an aqueous medium with 10% serum. However, fast aggregation was observed in the presence of 10 mM glutathione (GSH) due to detachment of the PEG segment via disulfide cleavage. These complexes showed high transfection efficiency in 293T and Hela cells under optimized conditions. The experimental results indicated that the mPEG-SS-PLL catiomers may have promising potential as a non-viral gene vector.
研究领域:
生物化学
·
分子生物学
·
基因治疗
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产品关联
DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-10
·
IF 5.228
Huang Wuren,Zhang Jie,Yang Bing,Beerntsen Brenda T.,Song Hongsheng,Ling Erjun
Invertebrate animals have the capacity of repairing wounds in the skin and gut via different mechanisms. Gastrointestinal perforation, a hole in the human gastrointestinal system, is a serious condition and surgery is necessary to repair the perforation to prevent an abdominal abscess or sepsis. Here we report the repair of gastrointestinal perforation made by a needle-puncture wound in the silkworm larval midgut. Following insect gut perforation, only a weak immune response was observed because the growth of Escherichia coli alone was partially inhibited by plasma collected at 6 h after needle puncture of the larval midgut. However, circulating hemocytes did aggregate over the needle-puncture wound to form a scab. While, cell division and apoptosis were not observed at the wound site, the needle puncture significantly enhanced DNA duplication in cells surrounding the wound, which was essential to repair the midgut perforation. Due to the repair capacity and limited immune response caused by needle puncture to the midgut, this approach was successfully used for the injection of small compounds (ethanol in this study) into the insect midgut. Consequently, this needle-puncture wounding of the insect gut can be developed for screening compounds for use as gut chemotherapeutics in the future.
研究领域:
昆虫学
·
医学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2019
·
IF 4.527
Cong Yuan,Lihong Wang,Long Zhu,Benhui Ran,Xue Xue,Zaizhao Wang
Ubiquitous BPA exposure resulted in DNA methylation errors and oxidative stress . Numerous studies have demonstrated that oxidative stress can lead to changes in DNA methylation levels and supplementation with antioxidants, including N-acetylcysteine (NAC), was able to restore these changes. Our previous study supposed that BPA-induced de novo synthesis of glutathione (GSH) promoted DNA methylation process in Gobiocypris rarus testes . To validate this conjecture and explore the protective effects of NAC on BPA toxicity, the present study was carried out. Adult male G. rarus was treated with 225 μg L −1 BPA and/or NAC for 7 days. The sperm motility and DNA integrity of G. rarus were determined. Meanwhile, the levels of 5-methylcytosine (5mC), GSH, hydrogen peroxide (H 2 O 2 ), DNA methyltransferase proteins (DNMTs), γ-glutamyl cysteine synthetase (GCS), S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), homocysteine (HCY), nicotinamide adenine dinucleotide phosphate (NADPH) and cysteine in the testes were detected. Furthermore, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were measured. Results indicated that NAC addition resulted in increase of cysteine contents and partially inhibited the BPA-induced DNA hypermethylation of G. rarus testes. In addition, the changes in DNA methylation levels in the testes after BPA and/or NAC treatment might be controlled by DNA methylation process that mediated by DNMTs. Moreover, BPA exposure caused oxidative stress in the testes and the elimination of H 2 O 2 might be mainly accomplished by CAT while it changed to mainly through GPx after NAC supplement. Finally, the positive response of testicular antioxidant enzyme system and the antioxidant activity of NAC itself protected sperm motility and DNA integrity from oxidative damage in each group.
研究领域:
环境科学
·
毒理学
·
分子生物学
·
水生生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-8
·
IF 6.232
Yang Zongqi,Liu Mei,Li Baoxin
A DNAzyme-powered DNA walking machine was constructed to develop the fluorescence aptasensing for sensitive detection of kanamycin. The aptamer for kanamycin is partially hybridized with complementary DNA (cDNA) modified on magnetic beads (MBs). The specific interaction of target and aptamer triggered the cDNA to be free tentatively, which captured walker DNA. Then the autonomous motion of DNA walker on MBs surface was propelled via DNAzyme digestion of recognition sites. The signal probe was separated, and the amplified fluorescence signal was achieved by the accumulation of the signal probe. Kanamycin was used as a model analyte, and the developed assay achieves a detection limit of 0.00039 ng·mL −1 (S/ N = 3) within a linear detection range from 0.001 to 2000 ng·mL −1 . This aptasensing strategy can be extended for detection of other antibiotics by adapting corresponding target recognition aptamer sequence.
Graphical abstract The fluorescence aptasensing for sensitive detection of kanamycin based on DNAzyme-powered DNA walking machine was constructed.
研究领域:
分析化学
·
生物传感器
·
分子诊断
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DOI
PubMed
高影响因子
NATURE BIOTECHNOLOGY
·
2025
·
IF 41.7
Zhang Xiandi,Jiang Xiang’er,Wang Yun,Chen Qinzhen,Jiang Hao,Zhang Hu,Beltran Antoni,Yang Weiya,Chen Tai,Liang Chenglong,Cheng Ning,Huang Yun,Ding Guqiao,Xie Chengwang,Gao Nanfeng,Liu Juntao,Xu Wei,Huang Jinlei,Cai Dong,Zhu Lihao,Mo Songjin,Shen Mengzhe,Zhang Wenwei,Lehner Ben,Ni Ming,Wang Jian,Xu Xun,Shen Yue
Current high-throughput DNA synthesis technologies use intricate chip and microfluidic systems to produce large-scale synthetic oligonucleotides but with low concentration and limited compatibility for long DNA assembly. Here we report a massive-in-parallel synthesis system, with an ‘identification–sorting–synthesis–recycling’ iteration mechanism applied to microchips for high-throughput DNA synthesis. This approach increases DNA product concentration by four to six orders of magnitude and simplifies downstream processes for large-scale gene synthesis.
研究领域:
分子生物学
·
合成生物学
·
生物技术
·
基因工程
·
高通量筛选
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产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2024
·
IF 8.7
Yibiao Wang,Xiaomei Fu,Yang Zhu,Mingjing Lin,Renduan Cai,Yang Zhu,Tiantian Wu
Intratumor bacteria, which are involved with complex tumor development mechanisms, can compromise the therapeutic efficiencies of cancer chemotherapeutics. Therefore, the development of anti-tumor agents targeting intratumor bacteria is crucial in overcoming the drug inactivation induced by bacteria colonization. In this study, a double-bundle DNA tetrahedron-based nanocarrier is developed for intratumor bacteria-targeted berberine (Ber) delivery. The combination of aptamer modification and high drug loading efficacy endow the DNA nanocarrier TA@B with enhanced delivery performance in anti-tumor therapy without obvious systemic toxicity. The loaded natural isoquinoline alkaloid Ber exhibits enhanced antimicrobial, anticancer, and immune microenvironment regulation effects, ultimately leading to efficient inhibition of tumor proliferation. This intratumor bacteria-targeted DNA nanoplatform provides a promising strategy in intervening the bacteria-related microenvironment and facilitating tumor therapy.
研究领域:
癌症治疗
·
纳米技术
·
药学
·
免疫学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Qian Liu,Zonghua Pu,Abdullah M. Asiri,Abdulrahman O. Al-Youbi,Xuping Sun
In this paper, a novel and highly sensitive DNA sensor based on biopolymer is presented. This polydopamine nanospheres-based sensing platform exhibits high selectivity and sensitivity with a detection limit of 5 nM and the sensitivity of this sensing platform increases with increased temperature.
研究领域:
生物传感器
·
材料科学
·
分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Nana Sun,Yuan Ding,Zhexuan Tao,Hongjie You,Xiude Hua,Minghua Wang
An upconversion fluorescence DNA probe which consists of aptamer-conjugated magnet nanoparticles (apt-MNPs) and complementary DNA-conjugated upconversion nanoparticles (cDNA-UCNPs) was developed to detect acetamiprid. Acetamiprid can specifically conjugate with the apt-MNPs to dissociate the cDNA-UCNPs from the apt-MNPs and resulted in reduced fluorescence intensity through an external magnet. The change of fluorescence intensity (△I) is positively related to the concentration of acetamiprid, which can be applied for the quantification of acetamiprid. Under optimal conditions, a linear detection range and detection limit are 0.89–114.18 μg/L and 0.65 μg/L, respectively. The probe was successfully used to detect acetamiprid in spiked paddy water, soil, pear, apple, wheat and cucumber. Average recoveries are 78.2%–103.5% with intra-day relative standard deviations (RSDs) of 2.6%–10.9% and inter-day RSDs of 4.3%–10.2%. The amounts of acetamiprid in the authentic paddy water and pear samples detected by the DNA probe are significantly correlated with that detected by high-performance liquid chromatography (HPLC).
研究领域:
分析化学
·
环境科学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jinzheng Zhang,Shuailong Zhou,Shiyi Tan,Kangyan Yi,Mengya Jin,Qian Shi,Fen Wu,Nannan Liu
Pore structure-based analytical techniques have great potential applications for the detection of biological molecules. However, the sophistication of traditional pore sensors is restricted in their applicability of analytical chemistry due to a lack of effective carrier probes. Here, we used porous coordination network-224 (PCN-224) composite probes in conjunction with a glass nanopipette (GN) as a sensing platform. The sensor exhibits a good fluorescence signal and a change in GN’s ionic current at the same time. Due to the volume exclusion mechanism coming from PCN-224, the detection limit of target DNA reaches 10 fM in a GN with a diameter of up to ca. 260 nm, outperforming a simple probe. The structure of the composite probe is optimized by the probe’s pairing efficiency. Furthermore, the sensor can also discriminate between 1-, 3-, and 5-mismatch DNA sequences and capture the target DNA from a complex mixture. Based on the GN platform, a series of techniques for detecting biomolecules are expected to emerge because of its simplicity, robustness, and universality by incorporating advanced nanoprobes.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Huimin Li,Xinru Yuan,Mei Ren,Jiajia Liu,Yixin Zheng,Ziyi Lin,Zixin Chen,Zhao Yang,Xin Su,Heyun Shen
Apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/ref-1, APE1), a vital protein for DNA repair and cellular redox regulation, is frequently overexpressed in tumor cells, underscoring the importance of developing sensitive detection methods for early cancer diagnosis. However, the rapid detection and visualization of nuclear APE1 in tumor cells are still challenging. In this study, we successfully developed a novel DNA fluorescent nanoprobe based on polyamidoamine (PAMAM) for the rapid detection of cytoplasmic and nuclear APE1. The PAMAM surface was modified with arginine (Arg), named PR, and its hydrophobic core encapsulated the 1,6,7,12-tetrachloroperylene tetracarboxylic acid dianhydride (TA) dye to construct fluorescent nanoparticles (TPR). Furthermore, an APE1-responsive dsDNA (SP) was linked on the TPR surface, containing apurinic/apyrimidinic sites (AP sites) and the black hole quencher 2 (BHQ2) and ensuring that fluorescence remains off in the absence of APE1. TPR-SP exhibited a detection range of 0.125–25 U mL–1 and a detection limit as low as 0.03 U mL–1. Compared with the Arg-free nanoprobes (TP-SP), TPR-SP significantly accelerated endocytosis and nuclear penetration, reducing the APE1 detection time to one-quarter (from 2 to 0.5 h). Notably, the APE1 signal in the whole nucleus can also be significantly detected. Thus, the TPR nanoprobe achieves the rapid enrichment and amplification of fluorescence signals, leading to highly sensitive whole cellular APE1 detection. This innovative and efficient detection method greatly expands the technological means for early cancer detection.
研究领域:
生物化学
·
分子生物学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
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