JOURNAL OF NATURAL PRODUCTS
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2022
·
IF 4.803
Jie Liu,Xiang Guo,Xingchen Guo,Boyuan Zhong,Tao Wang,Dong Liu,Hongwei Jin,Jinwei Ren,Zihe Liu,Jiangtao Gao,Shu-Ming Li,Aili Fan,Wenhan Lin
Spiromaterpenes are a group of rare tropone-containing sesquiterpenes with antineuroinflammatory activity. Herein, we elucidate their biosynthetic pathway in a deep-sea-derived Spiromastix sp. fungus by heterologous expression, biochemical characterization, and incubation experiments. The sesquiterpene cyclase SptA was first characterized to catalyze the production of guaia-1(5),6-diene, and a multifunctional cytochrome P450 catalyzed the tropone ring formation. These results provide important clues for the rational mining of bioactive guaiane-type sesquiterpenes and expand the repertoire of P450 activities to synthesize unique building blocks of natural products.
研究领域:
生物化学
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微生物学
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天然产物化学
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高影响因子
MOLECULES
·
2021
·Vol. 26
·期 23
·7189
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IF 4.412
Yue Li,Pei Li,Kailin Yang,Qian He,Yue Wang,Yuhua Sun,Chunnian He,Peigen Xiao
Sea buckthorn berries are rich in bioactive compounds and can be used for medicine and food. The variety and drying method used have an important influence on quality. In this study, different sea buckthorn varieties from China were selected and dried with four common drying methods. The total phenolic content (TPC), total flavonoids content (TFC), contents of 12 phenolic compounds and antioxidant capacity in vitro were analyzed. The results showed that the TPC, TFC and antioxidant activity of two wild sea buckthorn berries were higher than those of three cultivated berries, and for the same varieties, measured chemical contents and antioxidant activity of the freeze-dried fruit were significantly higher than those obtained with three conventional drying methods. In addition, forty-one compounds in sea buckthorn berry were identified by UPLC-PDA-Q/TOF-MS, most of which were isorhamnetin derivatives. Multivariate statistical analysis revealed narcissin and isorhamnetin-3-O-glucoside varied significantly in sea buckthorn berries of different varieties and with different drying methods; they were potential quality markers. Strong correlations were found between TPC, gallic acid and antioxidant capacity (p< 0.05). The results revealed how components and antioxidant activity varied in different sea buckthorn, which provides a valuable reference for quality control and further development and utilization of sea buckthorn.Keywords:sea buckthorn;drying method;variety;phenolic compounds;UPLC-Q-TOF-MS;antioxidant activity
研究领域:
食品科学
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药理学
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农业化学
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2021
·
IF 9.028
Ye Tian,Ke-Ke Wang,Gui-Peng Yang,Pei-Feng Li,Chun-Ying Liu,Riel Carlo O. Ingeniero,Hermann W. Bange
Nitric oxide (NO) is a short-lived intermediate of the oceanic nitrogen cycle, and it is produced by biological and photochemical processes in the ocean. Nitrogen dioxide (NO2) is a reactive atmospheric compound which has not been determined in the ocean so far. Here, we present the setup and validation of a novel continuous underway measurement system to measure dissolved NO and NO2 in the surface ocean. The system consists of a seawater/gas equilibration component coupled to a chemiluminescence detector. It was successfully deployed during a 12 day cruise to the East China Sea in May 2018. Dissolved NO and NO2 surface concentrations ranged from
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2020
·
IF 3.99
Guoli Xu,Zhi Zhang,Tao Li,Meiling Du,Yueping Guan,Chen Guo
In this study, a sea urchin-like Fe 3 O 4 /ZnO/ZnSe hierarchical heterostructured photocatalyst was successfully prepared via a convenient synthetic strategy and combined with a newly designed photocatalytic magnetically fixed bed reactor (PMFBR) to continuously photodegrade pollutants under simulated solar light irradiation. In the experiment, ZnO nanorods were first grafted onto the surfaces of hollow Fe 3 O 4 nanoparticles, and then ZnSe nanoparticles were uniformly distributed on the ZnO nanorods, the obtained Fe 3 O 4 /ZnO/ZnSe composite exhibited three-dimensional structure and excellent magnetic response. Through citing PMFBR, a stable and homogeneously distributed heterogeneous catalyst, efficient light distribution, and good mass transfer of reactants were achieved. As a result, the Fe 3 O 4 /ZnO/ZnSe composite in the PMFBR showed excellent photocatalytic capacity with a continuous process. The combination of the magnetic photocatalyst and PMFBR transformed the reaction mode from cumbersome intermittent operation to a simple continuous-flow photocatalytic style. It is hoped that this continuous photocatalytic system could provide a new idea for large-scale wastewater treatment.
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RSC Advances
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2023
·Vol. 13
·期 40
·27772-27781
·
IF 3.9
Tianrun Qian,Jia Bao,Xuepeng Liu,Gerile Oudeng,Weiwei Ye
17β-estradiol is abused in the food industry. Excess 17β-estradiol can disturb the endocrine system or cause many diseases including obesity, diabetes, cardiac-cerebral vascular disease, and cancers in the human body. A “turn-on” fluorescence resonance energy transfer (FRET) aptasensor based on carbon dots (CDs) and gold nanoparticles (AuNPs) was developed for the detection of 17β-estradiol. A thiol-modified oligonucleotide was conjugated to AuNPs and amino modified oligonucleotide was linked to CDs. The 17β-estradiol aptamer was hybridized with the two oligonucleotides, shortening the distance between CDs and AuNPs. With 360 nm UV light excitation, FRET occurred between CDs and AuNPs. The system was “turn-off”. When 17β-estradiol was detected, the aptamer specifically bound to 17β-estradiol, and the FRET system was destroyed, leading to the “turn-on” phenomenon. The fluorescence intensity recovery was detected in the concentration range of 400 pM to 5.5 μM. The limit of detection (LOD) was 245 pM. The FRET aptasensor demonstrated good selectivity for 17β-estradiol detection. Reasonable spiked recoveries were obtained in sea salt samples. It showed the potential for estrogen detection in food safety and environmental applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Shasha Huang,Kuiyou Wang,Zheng Hua,Abd El-Aty AM,Mingqian Tan
Liver cancer, a malignancy with a rising global incidence, poses a significant challenge in achieving effective treatment outcomes. As food-derived nutrient, sea cucumber peptide (SCP) has shown promising anticancer effects. Therefore, we explored the nanodelivery systems to encapsulate SCP to enhance its stability in the gastrointestinal tract and improve absorption within the tumor microenvironment. This study aimed to develop size-controllable multifunctional nanoparticles using SCP, procyanidins (PCs), and vanillin through molecular assembly via a one-pot Mannich condensation approach. These food-grade nanoparticles demonstrated water solubility and exhibited a spherical structure with sizes ranging from 441 to 1360 nm, depending on the concentration of the reactants. In vitro cell experiments demonstrated that SCP nanoparticles modified with PCs effectively reduced the generation of reactive oxygen species from H 2 O 2 and acrylamide while maintaining normal levels of mitochondrial membrane potential . Furthermore, in vivo nutrition intervention studies conducted on tumor-bearing mice revealed that mice treated with SCP nanoparticles exhibited a survival rate of 40 %, which was significantly higher than the 0 % and 20 % survival rates observed in the control and SCP-treated groups, respectively. These findings suggest that SCP nanoparticles, possessing antioxidative properties and controllable sizes, hold potential for precision nutrition in the field of cancer treatment.
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FOOD CHEMISTRY
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2023
·
IF 8.8
Meijuan Lan,Tongshuai Li,Lin Li,Shaoyun Wang,Juncheng Chen,Tangyu Yang,Zhiru Li,Yipeng Yang,Xia Zhang,Bing Li
The physiochemical properties, structure characteristics, oxidation, and emulsifying properties of myofibrillar proteins (MPs) in low salt solution after treated by the ultrasound were investigated. The solubility, mean diameters, sulfhydryl content, and carbonyl contents of MPs after ultrasonic treatment increased, while the turbidity decreased. The surface hydrophobicity of MPs with 200 W–600 W treatment increased, but decreased at 800 W treatment. The circular dichroism analysis revealed that α-helix content increased, while β-sheet and random coil content decreased after ultrasonic treatment. Fluorescence spectroscopy indicated the fluorescence intensities of MPs were increased after ultrasonic treatment. SDS-PAGE results showed more protein polymers due to myosin heavy chain (MHC) aggregation via disulfide bonds. Based on LC-MS/MS result, the myosin heavy chain was susceptible to oxidation, with monooxidation being the main oxidative modification. Finally, the emulsions stabilized by ultrasonically treated MPs, especially those treated at 800 W, exhibited decreased particle size, improved uniformity, and enhanced stability.
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DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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2023
·
IF 2.9
Yi-Xin Wu,Shuai-Yue Hu,Xin-Jiang Lu,Jian-Rao Hu
Piscidins participate in the innate immune response of fish, which aims to eliminate recognized foreign microbes and restore the homeostasis of immune system. We characterized two piscidin-like antimicrobial peptides (LjPL-3 and LjPL-2) isolated from Japanese sea bass ( Lateolabrax japonicus ). LjPL-3 and LjPL-2 showed different expression patterns in tissues. After Vibrio harveyi infection, the mRNA expression of LjPL-3 and LjPL-2 was upregulated in the liver, spleen, head kidney, and trunk kidney. The synthetic mature peptides LjPL-3 and LjPL-2 exhibited different antimicrobial spectra. Furthermore, LjPL-3 and LjPL-2 treatments decreased inflammatory cytokine production while promoting chemotaxis and phagocytosis in monocytes/macrophages (MO/MФ). LjPL-2, but not LjPL-3, displayed bacterial killing capability in MO/MФ. LjPL-3 and LjPL-2 administration increased Japanese sea bass survival after V. harveyi challenge, which was accompanied by a decline in bacterial burden . These data suggested that LjPL-3 and LjPL-2 participate in immune response through direct bacterial killing and MO/MФ activation.
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高影响因子
NEW JOURNAL OF CHEMISTRY
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2023
·Vol. 47
·期 14
·6958-6966
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IF 3.3
Xingyu Zhan,Hao Zhang,Yunxiong Zeng,Jingcai Xu,Ao Jin,Xinqing Wang,Jing Li,Yanting Yang,Bo Hong
Graphitic carbon nitride (CN) is considered to be a promising metal-free photocatalyst, but the charge carrier separation and rapid complexation of electrons and holes are difficult to modulate for effective and comprehensive photodegradation of TC. In this work, a MoS2 QD decorated sea-urchin carbon nitride nanohybrid was prepared via a water bath method. In comparison to the 85.6% degradation efficiency of sole sea-urchin carbon nitride (SUCN), the optimized 5% MoS2 QDs/SUCN (5%MSUCN) demonstrated a removal efficiency of 96.0% towards aqueous tetracycline (TC) under visible light. Such an outstanding photoactivity was mainly attributed to the interfacial synergistic effect of MoS2 QDs and SUCN, in which the outstanding charge extraction ability and quick surface kinetics of MoS2 QDs were primarily accountable for the subsequent deep photodegradation behavior of 5%MSUCN. A series of factors that may affect photoactivity of 5%MSUCN include pH values, inorganic ions, and organic matter. Finally, the deep photodegradation mechanisms of TC and intermediates were proposed based on ex situ HPLC-MS. This work not only explains the deep decomposition of TC but also presents a facile approach for designing and manufacturing carbon nitride nanohybrids.
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JOURNAL OF THE WORLD AQUACULTURE SOCIETY
·
2016
·Vol. 48
·期 2
·290-302
·
IF 0.665
Chenmin Liu,Yuzhe Han,Tongjun Ren,Zhiqiang Jiang,Fuqiang Wang,Mingling Liao,Jing Wang
A 56-d feeding trial was conducted to evaluate the effects of dietary lysine levels on growth performance, intestinal digestive enzymes, and nonspecific immune response of Apostichopus japonicus juveniles. Five semipurified diets were formulated to contain graded levels of lysine (from 0.28 to 2.32% of dry matter). Although no significant differences ( P > 0.05) were observed in survival rate, final weight (FW), and body weight gain (BWG), the FW and BWG increased from 0.28 to 1.19% with increasing dietary lysine levels, then showed a declining tendency. Broken-line regression analysis of BWG indicated sea cucumber requires lysine at 0.76% of dry diet. Proximate composition, lysine content of the body wall, and total amino acid profiles were not affected by dietary treatments. Intestinal protease level increased with increasing dietary lysine level from 0.28 to 1.19%, then decreased. Acid phosphatase and superoxide dismutase activities of the sea cucumbers were not affected by various dietary lysine levels; however, significantly ( P < 0.05) higher alkaline phosphatase activity was observed in sea cucumbers fed 2.32% lysine diet than those fed diets having 0.28 and 1.89% lysine. Overall, sea cucumber juveniles fed with diets containing between 0.76 and 1.19% lysine showed better growth performance, digestive enzymes, and nonspecific immune enzyme activities.
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ENVIRONMENTAL MICROBIOLOGY
·
2015
·Vol. 17
·期 10
·3795-3806
·
IF 6.201
Zi-Hao Mi,Zi-Chao Yu,Hai-Nan Su,Lei Wang,Xiu-Lan Chen,Xiuhua Pang,Qi-Long Qin,Bin-Bin Xie,Xi-Ying Zhang,Bai-Cheng Zhou,Yu-Zhong Zhang
Summary Although bacteriobenthos play a major role in the degradation of particulate organic matter in marine sediment, knowledge of the sediment-adapted lifestyles of bacteriobenthos is still scarce. Here, the particle-associated, swimming and swarming lifestyles of the benthonic bacterium P seudoalteromonas sp. SM9913 (SM9913) were illustrated. SM9913 had a clay particle-associated lifestyle, and its exopolysaccharide played an important role in this lifestyle. SM9913 also had swimming and swarming motilities, indicating that it may have swimming and swarming lifestyles in the sediment. The lateral flagella were responsible for the swarming motility, and the polar flagella were responsible for the swimming motility. Iron limitation was an indispensable inductive signal of the swarming motility. An analysis of the motilities of SM9913 and its mutants in clay demonstrated that SM9913 moved in clay by both swimming and swarming motilities. Genomic analysis suggests that having two flagella systems is most likely a common adaptation of some bacteriobenthos to the sediment environment. Our results reveal the lifestyles of benthonic SM9913, providing a better understanding of the environmental adaptation of benthonic bacteria.
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Journal of Energy Storage
·
2024
·
IF 8.9
Yu-Gang Fu,Yi-Han Li,Qiu-Feng Lü
Nickel‑cobalt layered double hydroxide (NiCo-LDH) has received much attention in the field of supercapacitor electrodes due to its multiple oxidation states and good electrochemical activity. However, NiCo-LDH often suffers from structural instability and low conductivity in practical applications. In this study, sea urchin-like structural NiCo-LDH coated cobalt‑nitrogen co-doped carbon (NiCo-LDH@Co-NC-x) electrode materials were prepared by in situ growth of NiCo-LDH on the surface of cobalt‑nitrogen co-doped carbon nanosheets (Co-NC) using a one-step hydrothermal method. The results show that the NiCo-LDH@Co-NC-2 electrode material exhibits excellent electrochemical performance, its specific capacitance is up to 1909 F g −1 at a current density of 1 A g −1 , and capacitance retention of 69.5 % (only 26.2 % for NiCo-LDH) can be achieved after 10,000 cycles at a current density of 10 A g −1 . This is because the abundant and uniformly distributed Co nanoparticles on Co-NC provide nucleation sites for the in-situ growth of NiCo-LDH, effectively improving the interface binding between Co-NC and NiCo-LDH, enhancing the structural stability of NiCo-LDH, and ensuring highly efficient electron transport throughout the NiCo-LDH@Co-NC-2 electrode. An asymmetric supercapacitor (NiCo-LDH@Co-NC-2//AC) was further assembled with NiCo-LDH@Co-NC-2 and activated carbon (AC) as positive and negative electrodes, respectively, achieving an energy density as high as 36.8 Wh kg −1 at a power density of 750 W kg −1 . These findings suggest that the NiCo-LDH@Co-NC-2 composite can be a promising candidate for high-performance supercapacitor electrode.
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