ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yi Xu,Waqas Ahmad,Md Mehedi Hassan,Huanhuan Li,Qin Ouyang,Quansheng Chen
A novel g-C 3 N 4 nanosheets (g-C 3 N 4 NSs)-assisted upconversion fluorescent aptasensor was proposed for Staphylococcus aureus ( S. aureus ) detection by adopting hybridization chain reaction (HCR) as a sensitizer. Two hairpin (H1 and H2) structured DNA probes were engineered predicated on the partial complementary sequence (cDNA) of S. aureus aptamer and modified on the exterior of the upconversion nanoparticles (UCNPs), respectively. The presence of S. aureus initiated the HCR system and activated H1 and H2 probes to form a double-helix away from the g-C 3 N 4 NSs vicinity. This led to the decrease in peroxidase-like activity (PA) of the g-C 3 N 4 NSs and corresponding fluorescence recovery proportional to the concentration of S. aureus (10-10 6 cfu mL −1 ). The method was applied to real food samples with acceptable recoveries (91.1–101.6%) and further validated by traditional plate counting method (p > 0.05).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Danyan Wang,Xiaojun He,Qiang Wang,Pingping Li,Enoch Obeng,Dongyang Zheng,Jianliang Shen,Zi Jin
Methicillin-resistant Staphylococcus aureus (MRSA) has been associated with significant mortality and longer hospital stays. Artificial nanozymes not requiring antibiotics can generate enough reactive oxygen species (ROS) to kill bacteria. In this study, we synthesized hexagonal copper sulfide nanosheets (CuS NSs) using a modified one-pot synthesis. The CuS NSs demonstrated photothermal stability and superior photothermal conversion efficiency due to large surface area and existence in the second near-infrared (NIR-II) window (1270 nm), promoting the production of ROS. Upon NIR-II irradiation, CuS NSs displayed an excellent biocatalytic antibacterial role in vitro and accelerated abscess resolution, and enhanced the recovery of MRSA-infected wounds in vivo . Moreover, CuS NSs demonstrated negligible toxicity following an amplifying peroxidase-like activity through hyperthermia. Overall, CuS NSs provide a feasible strategy for using the synergistic therapeutic platform for effective therapy of deep-seated MRSA-infected wounds.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Daorui Zhang,Lunjie Huang,Da-Wen Sun,Hongbin Pu,Qingyi Wei
Being a unique biocatalyst to hydrolyse structural polysaccharides on the cell wall of bacteria, lysozyme can slow down bacterial resistance caused by antibiotic overdose treatments, however, efficient activation of lysozyme activities under pathogenic microenvironments remains challenging. Herein, a bio-interface engineering strategy was proposed for the remote modulation of thermoresistant lysozyme upon rationally designed photothermal nanoplatforms. For this, Ti 3 C 2 T X MXene nanosheets were functionalized by polydopamine (PDA) surface chemistry to enhance photothermal effects and performance durability, during which lysozyme biomacromolecules were immobilized at such a two-dimensional hybrid interface via intermolecular electrostatic affinity. The integrated nanoplatform (denoted as [email protected] @Lyso), with an optimal high light-to-heat conversion efficiency of 46.88%, realized not only precision control of local heat but also photo-responsive up-regulation for bio-catalysis of laden lysozyme. As a result, in vitro and in vivo antibacterial experiments revealed that [email protected] @Lyso could effectively inhibit the proliferation of methicillin-resistant Staphylococcus aureus and accelerated wound disinfection of mice with negligible biological toxicities. The outstanding antibacterial activities of [email protected] @Lyso were attributed to the photo-enhanced lysozyme activity, assisted by bacterial death caused by the mild local hyperthermia and the physical destruction derived from the [email protected] This work exemplified a solution to the bacterial resistance threats via stimuli-responsive enzymatic nanoplatforms.
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Advances
·
2022
·
IF 0
Hongjun Yan,Peiyuan Li,Xiantao Jiang,Xiaoxun Wang,Yuting Hu,Ying Zhang,Rixiang Su,Wei Su
Curcumin (Cur) plays a key role in photodynamic antibacterial activity as a photosensitizer. On the other hand, the antimicrobial potential of graphene oxide (GO) has been reported controversially, and how to improve its antimicrobial ability has become an meaningful study. In this study, we prepared polydopamine-curcumin (PDA-Cur) by pi-pi stacking and loaded it onto the GO surface to obtain GO/PDA-Cur composite nanomaterials. GO/PDA-Cur was characterized by physical and optical means, and GO/PDA-Cur possessed good dispersion and stability in water. In vitro antibacterial results showed that GO/PDA-Cur mediated photodynamic therapy significantly reduced Gram-positive Staphylococcus aureus ( S. aureus ) by 4 orders of magnitude with a bactericidal rate of 99.99 %. The antibacterial mechanism stems from the fact that GO/PDA-Cur can generate reactive oxygen species (ROS) under white light irradiation (405–780 nm), which causes bacterial outer membrane breakage and cellular deformation. In addition, GO/PDA-Cur has good biocompatibility. The antibacterial ability of graphene oxide was significantly improved by combining it with PDA-Cur, which allows it to be used as a photodynamic antibacterial material.
✓ 已验证
产品关联
DOI
PubMed
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xiaoyuan Ma,Shan Xu,Liangyu Li,Zhouping Wang
Here, an aptamer-based SERS method for the detection of Staphylococcus aureus ( S. aureus ) without immobilization using [email protected] NPs/slide as enhanced substrate was constructed. ROX-aptamer of S. aureus was modified on the surface of [email protected] NPs/slide through electrostatic interaction. Based on the specific binding effect of the aptamer to S. aureus , the ROX-aptamer fell off from the surface of the substrate, resulting in a decrease of the SERS signal intensity of the substrate. Under the optimal experimental conditions, a good linear relationship was found between SERS intensity at 1500 cm −1 and the logarithm of concentration of S. aureus in the range of 10 2 cfu/mL-10 7 cfu/mL (y = 6623-796lgx, R 2 = 0.994) with a detection limit of 6 cfu/mL. The selectivity analysis revealed that the method had higher selectivity toward the corresponding target. The results for milk sample using the developed SERS method for the detection of S. aureus were similar to those of the plate counting method. The recovery ratio was from 90.60% to 107.26%, indicating the accuracy and reliability of the developed method. This method eliminates the need for bacterial immobilization and improves the convenience and efficiency of detection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Xin Wang,Min Zhang,Xiaoxu Pang,Kunlun Huang,Zhiyi Yao,Xiaohong Mei,Nan Cheng
Staphylococcus aureus is a frequent pathogenic bacterium that has a significant detrimental influence on the health of the human body. Therefore, developing a practical and portable detection platform is critical to ensuring food safety. Nanozymes are a kind of engineered nanomaterials with superior enzyme-like activities, providing infinite possibilities for the development of highly sensitive analytical assays. In this study, mesoporous core−shell [email protected] ( [email protected] ) nanozymes were synthesized and then applied as a signal amplifier in Staphylococcus aureus colorimetric immunoassay . At the same time, a careful comparative study of the catalytic performance with natural horseradish peroxidase (HRP), [email protected] nanozymes and their complexes [email protected] (HRP coupling with [email protected] nanozymes) were firstly performed, as well as clever using a colorimeter to achieve portable signal output. [email protected] bioprobes enable remarkable peroxidase-like catalytic activity, resulting in the highest sensitivity with a limit of detection (LOD) improved from 1 × 10 5 to 1 × 10 3 CFU/mL. In all, this work conducted a valuable comparative study and presented an improved strategy for the portable detection of pathogenic bacteria, which was expected to be used in a wide range of applications in food safety regulation and biomedicals arenas.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Analysis and Testing
·
2022
·Vol. 6
·期 2
·111-119
·
IF 0
Guo Xin,Deng Xin-Chun,Zhang Ya-Qing,Luo Qin,Zhu Xiao-Kang,Song Yang,Song Er-Qun
Rapid and accurate detection of pathogenic bacteria of Staphylococcus aureus ( S. aureus ) is of importance in the field of clinical research and practical application. Herein, we report a magnetic relaxation switching (MRS)-based strategy for detecting S. aureus in complex samples via the assistance of signal amplification system of liposome. In this design, vancomycin (Van) modified 200 nm magnetic beads (MB 200 -Van) and biotinylated immunoglobulin (Biotin-IgG) were employed as capture probes for S. aureus which following labelled by biotinylated liposomes loading with glucose molecule (Biotin-liposome@Glu, BLGs) based on the affinity of biotin with streptavidin. The released glucose molecules combining with glucose oxidase generated Fe 3+ , causing transverse relaxation time ( T 2 ) signal change. The combination of dual recognition of S. aureus , liposome assisted signal amplification, and the MRS-based assay achieved direct and specific detection of S. aureus in blood and abscess samples. The as-proposed method for S. aureus assay in this study shows potential application for infectious diagnosis in clinical.
✓ 已验证
产品关联
DOI
JOURNAL OF NANOBIOTECHNOLOGY
·
2019
·Vol. 17
·期 1
·1-14
·
IF 5.345
Gasmalla Hind Baballa,Lu Xiaoquan,Shinger Mahgoub Ibrahim,Ni Lubin,Chishti Aadil Nabi,Diao Guowang
Background Iron oxide nanocomposites have received a great attention for their application in various fields like physics, medicine, biology, and material science etc., due to their unique properties, such as magnetism, electrical properties, small size, biocompatibility and low toxicity. Methods Fe 3 O 4 /Ag 3 PO 4 @WO 3 nanocomposites with different weight percent of Ag 3 PO 4 were successfully prepared through fabricated Ag 3 PO 4 /Fe 3 O 4 with WO 3 via in situ fabrication method, electrospinning involved precursor solution preparation and spinning to enhance photocatalyst performance under simulated sunlight for the degradation of methylene blue (MB) and antibacterial activity against Staphylococcus aureus ( S. aureus ). Results The photocatalytic degradation of methylene blue (MB) under simulated light irradiation indicated that the nanocomposite with 0.25 mg of Ag 3 PO 4 has the best activity. An additional advantage of these photocatalysts is magnetic recoverability, using external magnetic field and photocatalytic stability of the nanocomposites was evaluated for three cycles. In addition, using different scavengers, holes (h + ) and superoxide radical (O
·−) 2
radicals and hydroxide radical ( · OH) were identified the main oxidative species in the degradation reaction of methylene blue. Conclusions The results reveal that Fe 3 O 4 /Ag 3 PO 4 @WO 3 -0.25 nanocomposites have photocatalytic and antibacterial activity against S. aureus. The photocatalyst and mechanism based on the enhancement of electron transfer processes between Ag 3 PO 4 and WO 3 nanoparticles.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 30
·17002-17015
·
IF 3.049
Kishore Chand,M. Ishaque Abro,Umair Aftab,Ahmer Hussain Shah,Muhammad Nazim Lakhan,Dianxue Cao,Ghazanfar Mehdi,Abdalla Mohamed Ali Mohamed
Recently, it has been shown that silver nanoparticles (AgNPs) exhibit great potential for different applications, including food storage, cosmetic products, electronic components, biosensor materials, cryogenics, dental materials and especially for drug-delivery activities. In this study, we synthesized AgNPs with neem extract (NE) alone and mixed plant extracts of neem, onion and tomato (NOT) as a combined reducing and stabilizing agent by a green synthesis method at different pHs. The synthesized products were characterized by ultraviolet-visible spectroscopy (UV-vis), X-ray diffraction (XRD), dynamic light scattering (DLS), atomic force microscopy (AFM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The antibacterial effects of the synthesized products were studied by the Kirby disk diffusion method. It was confirmed that the AgNPs work effectively as a drug material against Gram-positive bacteria Staphylococcus aureus in nutrient agar. In addition, it was seen that the reducing and stabilizing agent NOT could work effectively with six medicines with a different nature at the maximum addition of 15 μg. However, the synthesized product with NE alone only worked for four of the medicines. Therefore, it was evident that the AgNPs synthesized with NOT extract were more susceptible to the Gram-positive bacteria Staphylococcus aureus. We believe that this new route for synthesizing AgNPs with NOT extract could be more beneficial in comparison to NE alone for improved antibacterial properties in drug-delivery applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2018
·
IF 3.667
Zheng Fang,Liangzong Xu,Yanlan Lin,Xixi Cai,Shaoyun Wang
A new type of zinc-chelating peptide was fabricated from Octopus scraps protein hydrolysate and its antibacterial activity against Staphylococcus aureus was characterized. Results of Fluorescence spectra and Fourier Transform Infrared Spectrometer (FT-IR) demonstrated that the amino nitrogen atoms and the oxygen atoms belonging to the carboxylate groups were the primary chelating sites for zinc ions. The zinc-peptide chelate exerted remarkably antimicrobial ability towards S. aureus , whose minimum inhibitory concentration (MIC) was 1.56 mg/mL. To investigate the antibacterial mechanism, changes of bacterial morphology, cell constituents, membrane potential, intracellular reactive oxygen species (ROS) level and intracellular enzyme activity were measured. Results revealed that Zn-peptide exerted its bactericidal activity by damaging bacterial cell membranes and cell walls, increasing the cell permeability which resulted in structural lesions and release of cell components. Besides, Zn-peptide induced S. aureus cell death via ROS triggered pathway. These findings further promote the development of Zn-peptide complexes, suggesting that Zn-peptide chelate has potential as natural bacteriostatic agent in food processing industry.
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 38
·6538-6545
·
IF 2.7
Shanglin Li,Zhaomeng Sun,Ziyang He,Mei Liu
A colorimetric aptasensor based on the dual recognition of magnetic carbon dots (M-CDs) and copper-based metal–organic frameworks (Cu-MOFs) was constructed for the visual detection of Staphylococcus aureus (S. aureus). Aptamer-modified Cu-MOFs could specifically identify target bacteria and exhibited peroxidase-like activity by catalyzing the colorimetric reaction of the 3,3′,5,5′-tetramethylbenzidine–H2O2 (TMB–H2O2) system. M-CDs electrostatically attracted bacteria because of their strong positive electrical properties and offered better capturing performance compared with magnetic beads owing to their smaller size. Visual detection of the target was achieved using the color change of the reaction catalyzed by the resuspension of the sandwich-structured complex. The bacteria were sensitively detected with a limit of detection (LOD) of 1 CFU mL−1 using the peroxidase catalytic activity of Cu-MOFs. Non-target bacteria produced negative results, demonstrating the excellent selectivity of the proposed aptasensor. The strategy showed good recovery in real sample detection, showing great potential for visual monitoring of pathogenic bacteria, and could provide a reference for further development of novel sensing platforms for bacterial detection.
研究领域:
生物化学
·
分析化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2022
·Vol. 10
·期 5
·
IF 9.043
Wen Wang,Xuerui Bao,Mona Bové,Petra Rigole,Xiaofeng Meng,Jianyu Su,Tom Coenye
Phytochemicals are promising antibacterials for the development of novel antibiofilm drugs, but their antibiofilm activity in physiologically relevant model systems is poorly characterized. As the host microenvironment can interfere with the activity of the phytochemicals, mimicking the complex environment found in biofilm associated infections is essential to predict the clinical potential of novel phytochemical-based antimicrobials. In the present study, we examined the antibiofilm activity of borneol, citral, and combinations of both as well as their Pickering emulsions against Staphylococcus aureus and Pseudomonas aeruginosa in an in vivo-like synthetic cystic fibrosis medium (SCFM2) model, an in vitro wound model (consisting of an artificial dermis and blood components at physiological levels), and an in vivo Galleria mellonella model. The Pickering emulsions demonstrated an enhanced biofilm inhibitory activity compared to both citral and the borneol/citral combination, reducing the minimum biofilm inhibitory concentration (MBIC) values up to 2 to 4 times against P. aeruginosa PAO1 and 2 to 8 times against S. aureus P8-AE1 in SCMF2. In addition, citral, the combination borneol/citral, and their Pickering emulsions can completely eliminate the established biofilm of S. aureus P8-AE1. The effectiveness of Pickering emulsions was also demonstrated in the wound model with a reduction of up to 4.8 log units in biofilm formation by S. aureus Mu50. Furthermore, citral and Pickering emulsions exhibited a significant degree of protection against S. aureus infection in the G. mellonella model. The present findings reveal the potential of citral- or borneol/citral-based Pickering emulsions as a type of alternative antibiofilm candidate to control pathogenicity in chronic infection.IMPORTANCE There is clearly an urgent need for novel formulations with antimicrobial and antibiofilm activity, but while there are plenty of studies investigating them using simple in vitro systems, there is a lack of studies in which (combinations of) phytochemicals are evaluated in relevant models that closely resemble the in vivo situation. Here, we examined the antibiofilm activity of borneol, citral, and their combination as well as Pickering emulsions (stabilized by solid particles) of these compounds. Activity was tested against Staphylococcus aureus and Pseudomonas aeruginosa in in vitro models mimicking cystic fibrosis sputum and wounds as well as in an in vivo Galleria mellonella model. The Pickering emulsions showed drastically increased antibiofilm activity compared to that of the compounds as such in both in vitro models and protected G. mellonella larvae from S. aureus-induced killing. Our data show that Pickering emulsions from phytochemicals are potentially useful for treating specific biofilm-related chronic infections.
研究领域:
微生物学
·
药理学
·
感染病学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）