SPECTROSCOPY LETTERS
·
2025
·
IF 1.6
Na Ni,Yanhua Tang,Nian Ding,Kang Peng,Xinwang Zhang,Qianqian Su
This study used the acidic anionic dye eosin Y (EY) as a resonance Rayleigh scattering (RRS) probe to develop a method for the detection of doxorubicin hydrochloride (DOX). In dilute hydrochloric acid solution medium, eosin Y interacted with each other through hydrogen bonding to form supramolecular aggregates. The addition of doxorubicin hydrochloride induced an ion-conjugation process and dissociated the hydrogen-bonded supramolecular aggregates that eosin Y had formed. As a result, the RRS signal of eosin Y at 534 nm was significantly diminished. The reaction conditions were optimized and the mechanism of RRS intensity reduction was explored. It was found that the main reason for the weakening of the RRS intensity was the decrease in particle size. The range of 0.5–5.0 mg/L is well-represented by the concentration of doxorubicin hydrochloride, which shows decent linear relationship. The linear regression equation was ΔI = 15044c + 10465 with a correlation coefficient R of 0.9995, and a limit of detection of 0.085 mg/L. The recoveries ranged from 97.33% to 102.94%. This work provided a new, sensitive, and cost-effective method for estimating doxorubicin hydrochloride, which can be successfully applied to the estimation of doxorubicin hydrochloride for injection.
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JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Baoshan Tang,Fen Xiong,Lanxiang Liu,Juan Xu,Jinju Ma,Hong Zhang
As an antibiotic, the imbalance of the concentration of doxycycline hydrochloride (DOX) can cause many harms, so real-time detection of the DOX content is crucial. In this study, Galla chinensis residue (GCR) was used as a carbon source, m-phenylenediamine was used as an N-doping agent, and blue-green fluorescent carbon dots (N-GCRCDs) were prepared by a simple hydrothermal synthesis method, with a high quantum yield of 27.31 %. Under optimized conditions, a specific fluorescent probe was established to detect DOX. The addition of DOX resulted in a significant decrease in fluorescence intensity, with a detection range of 5.7–114.9 μM and the detection limit was 1.38 μM. Finally, the feasibility of this method was validated in human urine and the experimental results exhibited a DOX recovery rate of 97.45–104.41 % and an RSD of 0.91–2.64 %. The quenching mechanism was mainly attributed to the inner filter effect and static quenching. Furthermore, the potential application of N-GCRCDs in cells was studied and biocompatibility was evaluated using HepG2 cells, which showed low toxicity at concentrations of 0–50 μg/mL and successful application for HepG2 cell imaging. This study provides reference for resource utilization of GCR and quantitative detection of antibiotics.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2025
·
IF 5.2
Li Xiang,Xianjing Xiang,Qiangqiang Jiao,Yu Luo,Guodong Zeng,Wenhui Zhang,Yuting Qin,Yuping Chen
Rapid proliferation underlies the abnormal expansion of activated hepatic stellate cells (aHSCs) and thereby contributes to the development and progression of liver fibrosis, so inhibition of HSC proliferation serves as a good antifibrotic strategy. As a potent topoisomerase II inhibitor, doxorubicin (DOX), an antineoplastic drug, exhibits a significant antifibrotic activity in vitro via retarding the growth of aHSCs and reversing their myofibroblastic phenotype, but its severe hepatotoxicity, cardiotoxicity, and renal toxicity limit its wide clinical application. Therefore, enhancing the specificity and efficacy of DOX in targeting aHSCs to improve its therapeutic index and minimize its adverse effects has become a key point for the success of DOX in antifibrotic treatment. In this study aimed at liver fibrosis treatment, we combined the excellent drug-loading capability and good biocompatibility of black phosphorus nanosheets (BPNSs), the protective and camouflaging properties of red blood cell membrane encapsulation, and the HSCs-targetability provided by the surface modification with vitamin A derivatives, into the construction of HSCs-targeted BP/DOX nanovesicles (BP/DOX@RMV-VA). The obtained DOX nanovesicles exhibited a uniform particle size and spheroid morphology, excellent diffusion property and stability, and high DOX loading. Specifically, they demonstrated outstanding biosafety, effective HSCs-targetability both in vivo and in vitro, and markedly improved pharmacokinetic profile of DOX. BP/DOX@RMV-VA produced strong antiproliferative and MF-phenotype reverting activity both in cultured aHSCs and in mice chronically injured by CCl 4 . And accordingly, the administration of BP/DOX@RMV-VA to CCl 4 -injured mice effectively suppressed the expansion of aHSCs and fibrogenesis, and significantly improved liver structure and function without causing detectable cardiotoxicity. These results highly suggest the therapeutic potential of BP/DOX@RMV-VA in treating liver fibrosis and other fibrosis-associated liver diseases.
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RSC Advances
·
2018
·Vol. 8
·期 4
·2082-2091
·
IF 2.936
Yangui Chen,Xiaoxia Li,Hong Xiao,Jinpeng Xiao,Bo Li,Xiaoyan Chen,Yong Wang,Du Cheng,Xintao Shuai
Owing to the complexity of tumorgenesis, combination therapy has proven to be a viable strategy for cancer treatment in recent years. However, the delivery and site-specific release of different therapeutic agents remain a major challenge in combination therapy. In this study, a polymeric nanovesicle based on a copolymer of polyethylene glycol and a polypeptide derivative was introduced as a vector to simultaneously deliver hydrophobic gefitinib and hydrophilic doxorubicin hydrochloride for multi-target combination therapy. The vesicle incorporating the two drugs exhibited prominent pH/reduction sensitivities to trigger the release of gefitinib and doxorubicin inside cancer cells. The two drugs co-delivered by the polymeric nanovesicle exhibited a joint anticancer effect both in vitro and in vivo. In particular, a remarkable therapeutic effect was demonstrated in animal studies using a mouse N2a neuroblastoma model. This study reveals the potential of reduction and pH dual-responsive nanovesicles bearing gefitinib and doxorubicin as an effective nano-medicine for cancer treatment.
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Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 15
·2217-2229
·
IF 4.776
Shengnan Huang,Ying Liu,Xin Xu,Mengfei Ji,Yuanmin Li,Chengjun Song,Shaofeng Duan,Yurong Hu
A combination of different therapy strategies has great potential to efficaciously treat malignant tumors, by virtue of their synergetic effects. Herein, a co-delivery system based on gold nanocages (AuNCs) was designed to deliver both doxorubicin (DOX) and microRNA-122 mimic (miR-122) for an enhanced cancer therapy. DOX was loaded into the AuNCs and miR-122 was condensed onto the surface of the functionalized AuNCs by an electrostatic interaction. Polyethyleneglycol (PEG) and hyaluronic acid (HA) were also introduced to the co-delivery system for targeted drug delivery. We evaluated the cellular uptake, biodistribution and anti-tumor effect in vitro and in vivo. Our results demonstrated an effective delivery of DOX and miR-122 into tumor cells and the tumor tissue. Importantly, the triple therapy, namely the combination of chemotherapy, gene therapy and photothermal therapy, mediated by this multifunctional drug delivery system, exhibited better anti-tumor effect than any single therapy, both in vitro and in vivo. Additionally, this drug delivery system caused insignificant toxicity to the major organs and had no obvious effect on the body weight of the mice. It could be concluded that multifunctional AuNCs are promising as a co-delivery vector for an enhanced anti-tumor effect.
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TALANTA
·
2024
·
IF 5.6
Songjia Luo,Qingliu Wu,Lu Wang,Hao Qu,Lei Zheng
Electrochemical aptamer-based biosensors (EABs) have been developed for multiple important biomarkers for their convenient and real-time features. However, the application of EABs in complex biological fluids has been limited by the rapid loss of sensitivity and selectivity due to inactivation and biofouling of aptamer probes and electrodes. To address this issue, we report the preparation of a simple hydrogel-protected aptamer-based biosensor (HP-EAB) for direct detection of Doxorubicin (DOX) in whole blood. The aptamer provides excellent selectivity for the electrochemical sensor, allowing the prepared sensor to accurately detect DOX in a 50-fold diluted whole blood sample. The agarose hydrogel coating on the electrode surface allows the passage of small molecules while hindering the adsorption of biomolecules from the whole blood matrix to the electrode surface. The experimental results show that the prepared HP-EAB has high stability compared with the unprotected EAB, and the HP-EAB maintains excellent detection performance after 7 days of storage. The hydrogel coating can effectively reduce the non-specific response to the whole blood matrix and prolong the life-time of the sensor. When used to detect DOX in rabbit whole blood, the HP-EAB exhibited excellent detection performance with a detection limit of 25.9 nM (S/N = 3) and a detection range of 0.1 μM–50 μM. The developed HP-EAB provides an excellent platform for the rapid and accurate determination of important analytes in complex biological fluids.
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BIOELECTROCHEMISTRY
·
2024
·
IF 4.8
Yunzhi Zhao,Ying Hao,Min Cui,Na Li,Bao Sun,Yu Wang,Haiyan Zhao,Cong Zhang
Doxorubicin (DOX) is a clinical chemotherapeutic drug and patients usually suffer from dose-dependent cytotoxic and side effects during chemotherapy process with DOX. Therefore, developing a reliable strategy for DOX analysis in biological samples for dosage guidance during chemotherapy process is of great significance. Herein, a sensitive and selective electrochemical biosensor for DOX detection was designed based on gold nanoparticles (AuNPs) and DNA tetrahedron (TDN) nanoprobe bifunctional glassy carbon electrode that could detect DOX in human serum and cell lysate samples. AuNPs not only could enhance electron transfer efficiency and detection sensitivity, but also could improve the biocompatibility of electrode. TDN nanoprobes were employed as specific DOX bind sites that could bind abundant DOX through intercalative characteristics to contribute to sensitive and selective detection. Under the optimal conditions, the proposed TDN nanoprobes-based DOX biosensor exhibited a wide linear range that ranged from 1.0 nM to 50 μM and a low detection limit that was 0.3 nM. Moreover, the proposed DOX biosensor displayed nice selectivity, reproducibility and stability, and was successfully applied for DOX detection in human serum and cell lysate samples. These promising results maybe pave a way for DOX dosage guidance and therapeutic efficacy optimization in clinic.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 2
·1-8
·
IF 5.3
Ou Dan,Yan Huixian,Chen Zuanguang
Based on the electrochemical impedance method, a marker-free biosensor with aptamer as a biometric element was developed for the determination of doxorubicin (DOX). By combining aptamer with rigid tetrahedral DNA nanostructures (TDNs) and fixing them on the surface of gold electrode (GE) as biometric elements, the density and directivity of surface nanoprobes improved, and DOX was captured with high sensitivity and specificity. DOX was captured by immobilized aptamers on the GE, which inhibited electron transfer between the GE and [Fe(CN)6] 3− / 4− in solution, resulting in a change in electrochemical impedance. When the DOX concentration was between 10.0 and 100.0 nM, the aptasensor showed a linear relationship with charge transfer resistance, the relative standard deviation (RSD) ranged from 3.6 to 5.9%, and the detection limit (LOD) was 3.0 nM. This technique offered a successful performance for the determination of the target analyte in serum samples with recovery in the range 97.0 to 99.6% and RSD ranged from 4.8 to 6.5%. This method displayed the advantages of fast response speed, good selectivity, and simple sensor structure and showed potential application in therapeutic drug monitoring. Graphical Abstract
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Guanyu Ruan,Lixiang Ye,Jianxi Ke,Hongyu Lin,Minxia Wu,Zhihong Liu,Yu Fang,Shuihua Zhang,Hongmei Wang,Yongsheng Liu,Hongtao Song
Efficiently overcoming chemoresistance in tumor microenvironments remains a critical hurdle in cancer therapy due to tumor heterogeneity, limited drug penetration, and adaptive resistance mechanisms. Herein, we report the design and application of all-in-one gadolinium–doxorubicin nanoassemblies (GDNAs) for spatially targeted delivery and chemoresistance reversal. These multifunctional nanoassemblies integrate a lanthanide-based component for real-time imaging and doxorubicin for chemotherapy, coupled with bioinformatics-guided small interfering RNAs (siRNAs) to silence key resistance-associated genes such as BCL2 and BIRC5. The GDNAs demonstrate enhanced tumor penetration and specificity for chemoresistant cells, achieving deep tissue delivery and synergistic effects in human-derived organoids and xenograft breast cancer models. Remarkably, GDNAs significantly reduce tumor viability and growth while attenuating invasive potential, showcasing superior therapeutic efficacy compared to conventional treatments. Comprehensive preclinical evaluations confirm their biocompatibility and low systemic toxicity, underscoring the translational potential of this platform. This work introduces a paradigm-shifting strategy by integrating imaging, targeted therapy, and gene silencing to address chemoresistance, offering a versatile approach for personalized cancer treatment.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2017
·
IF 2.764
Bei Xie,Jipeng Yi,Jian Peng,Xing Zhang,Lei Lei,Dapeng Zhao,Zhixin Lei,Hemin Nie
Co-delivery of chemical drugs and therapeutic genes for synergistic therapy provides a promising strategy to treat devastating diseases. However, the real-time coordination patterns between chemical drugs and therapeutic genes remain poorly understood. Herein, the complexes of doxorubicin/graphene oxide-polyethyleneimine/p53 plasmid (Dox/GO-PEI/p53) were fabricated and employed to investigate the synergistic manner between Dox and p53 in the inhibition of HeLa cell growth. GO was conjugated with PEI to form the GO-PEI backbone as the delivery vector. The GO backbone provided surfaces with a high specific area to load Dox via the π-π stacking interaction, and was able to release Dox significantly faster at pH 5.0 than at pH 7.0, while the positively charged PEI section of GO-PEI could condense plasmids into GO-PEI/DNA nanoparticles via the electrostatic interaction . The nanoparticles efficiently mediated the transfection of DNA in HeLa cells, with lower cytotoxicity compared to PEI/DNA nanoparticles. Furthermore, the complexes of Dox/GO-PEI/p53 released Dox and expressed p53 gene in a sequential manner, and showed successive inhibition of the in vitro growth of HeLa cells. This type of drug/GO-PEI/DNA complex can be employed as a platform to investigate the coordination pattern between chemical drugs and therapeutic genes for tumor therapy.
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BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
·
2024
·
IF 1.9
Lin Shuai,Cai Hui,Song Xuemei
The objective of this study was to explore the effects and mechanisms of the combination of isobavachalcone (IBC) and doxorubicin (DOX) on the progression of anaplastic thyroid cancer (ATC). Cell viability of 8505C and CAL62 cells was observed by CCK-8 assay. Kits were used to detect the presence of reactive oxygen species (ROS), glutathione (GSH), malondialdehyde (MDA), and cellular iron. Protein expression of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) was detected using western blot, and CD31 was detected through immunofluorescence. Tumor xenograft models of 8505C cells were constructed to observe the effect of IBC and DOX on ATC growth in vivo. The co-administration of IBC and DOX exhibited a synergistic effect of suppressing the growth of 8505C and CAL62 cells. The concurrent use of IBC and DOX resulted in elevated iron, ROS, and MDA levels, while reducing GSH levels and protein expression of SLC7A11 and GPX4. However, the Fer-1 ferroptosis inhibitor effectively counteracted this effect. In vitro and in vivo, the inhibitory effect on ATC cell proliferation and tumor growth was significantly enhanced by the combination of IBC and DOX. The combination of IBC and DOX can inhibit the growth of ATC by activating ferroptosis, and might prove to be a potent chemotherapy protocol for addressing ATC.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Huichen Zhao,Yaping Wang,Junmin Qian,Ming Yang,Chenyang Liu,Weijun Xu,Xinyu Li,Mofan Xiao,Wenjuan Luo,Aili Suo
Combination of different therapy modalities has emerged as a promising strategy for effective cancer treatment. Herein, we developed a multifunctional doxorubicin (DOX)-conjugated hyaluronic acid/polypyrrole (HA/PPy) nanoplatform, termed PPy-HA DOX -CH t nanoparticles, for the targeted photothermal chemotherapy of breast cancer. This nanoplatform was fabricated through layer-by-layer assembly of dopamine/hydrazide-modified hyaluronic acid (DHHA), hydroxyethyl chitosan, and anti-HER2 antibody-conjugated hyaluronic acid aldehyde on PPy nanoparticles. DHHA was synthesized by successively grafting dopamine and 3,3′-thiodipropionodihydrazide onto the carboxyl groups of HA via carbodiimide reaction for subsequent DOX conjugation through acid-labile hydrazone linkages. We systematically investigated the preparation, physicochemical properties, and anticancer efficacy of the nanoplatform. The results show that the size of PPy nanoparticles synthesized via Fe 3+ -initiated oxidation polymerization is controllable. The nanoplatform exhibits pH/glutathione dual-responsive drug release feature, excellent photothermal performance, enhanced structural stability, and outstanding biocompatibility. In vitro studies reveal that the nanoplatform can be efficiently taken up by MCF-7 cells via HER2-mediated endocytosis and kill them via apoptosis through the combination of PPy-mediated near infrared photothermal therapy and DOX-based chemotherapy. In vivo evaluation demonstrates significant tumor growth suppression. In a word, the as-prepared multifunctional nanoplatform holds substantial potential for targeted combination therapy of breast cancer.
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