ENGINEERING IN LIFE SCIENCES
·
2012
·Vol. 13
·期 2
·194-200
·
IF 1.925
Yuanyuan Yu,Jiugang Yuan,Qiang Wang,Xuerong Fan,Ping Wang,Xuejiao Sun
Cellulases can penetrate into the fiber, causing tensile strength loss of the cellulosic fibers or fabrics. To minimize the tensile strength loss, we have immobilized cellulases on Eudragit S-100. The characteristics of covalent Eudragit cellulase were evaluated using gel filtration analysis and UV spectra. Gel filtration analysis revealed that the cellulases were covalently bound to the polymer. Covalent Eudragit cellulase was loaded with the enzyme of about 40% and had a relative activity about 80% at a Eudragit S-100 concentration of 15 g/L. When cellulase is bound to the polymer, the solubility profile becomes similar to the one of Eudragit. In addition, the effects of the enzyme on the cotton yarns and fabric using cellulases have been investigated. Native and immobilized cellulases caused improvements in whiteness and wrinkle recovery angle of the fabric in comparison to the control samples. The bending stiffness results show that native and immobilized cellulase treated cotton fabric has an improved softness than the control samples. It was found that using the immobilized cellulase reduced the weight and tensile strength, because the hydrolytic attack is only limited to the surfaces of cotton fibers.
研究领域:
生物化学
·
纺织科学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2013
·Vol. 1
·期 17
·2260-2267
·
IF 0
Ying Shen,Wei Guo,Li Qi,Juan Qiao,Fuyi Wang,Lanqun Mao
The effort to stabilize enzymes and improve their activity has generated great interest because of their wide application in proteomics research, bioenergy conversion, bioassays and so on. In this work, biocompatible reactive polymer, poly (glycidyl methacrylate), grafted from magnetic nanoparticles by atom transfer radical polymerization method, has been firstly proposed to immobilize enzymes for microwave-assisted digestion. Meanwhile, trypsin was chosen as a model enzyme. Resulting from the increased functionality, the immobilization amount of the enzyme on the magnetic nanoparticle surface has been greatly improved. Furthermore, the enzyme immobilized magnetic nanoparticles have exhibited excellent repeatability and stability. The influence of the polymer chain length on digestion efficiency has been investigated both at 37 °C and under microwave. It has been found that the digestion efficiency increases with the lengthened polymer brushes due to the increased immobilization amount. Utilizing cytochrome C as a model protein for digestion, the performance of this immobilized biocatalyst has been demonstrated and this digestion assisted with microwave could be completed within 15 s. This study offers insight into the design of polymer brushes on the surface of magnetic nanoparticles for high digestion efficiency in the future.
研究领域:
生物化学
·
材料科学
·
酶工程
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DOI
PubMed
INDUSTRIAL CROPS AND PRODUCTS
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2023
·
IF 5.9
Chaozhong Xu,Liqun Sun,Shanshan Tong,Jia Ouyang,Xiaoli Gu
This study investigates the cellulase immobilization onto zeolitic imidazolate frameworks (ZIFs) to enable efficient cellulose hydrolysis at high solids loading. Different ZIF carriers (ZIF-8, ZIF-8-NH 2 , Fe 3 O 4 @ZIF-8, and Fe 3 O 4 @ZIF-8-NH 2 ) were prepared and characterized. The resulting ZIF-immobilized cellulases were subsequently analyzed for their loading capacity and enzyme activity , as well as their biochemical properties such as temperature stability, pH stability, storage stability, enzyme leaching, and enzyme kinetics . Results revealed that the cellulase immobilized on Fe 3 O 4 @ZIF-8-NH 2 exhibited an exceptional loading capacity of 359.89 mg/g and a relative enzyme activity of 69.39%. Remarkably, at a solid loading of 15% (w/w) and an enzyme dosage of 5 FPU/g cellulose, the Fe 3 O 4 @ZIF-8-NH 2 immobilized cellulase demonstrated a 20.44% higher hydrolysis yield compared to the free cellulase. Furthermore, this immobilized enzyme system displayed robust reusability, retaining 71.03% of its initial activity even after 10 cycles of reuse. A proposed mechanism of enhanced enzymatic hydrolysis by ZIF-immobilized cellulases was also presented. These findings have significant implications for the future application of ZIF-immobilized cellulases in efficient and cost-effective lignocellulosic bioconversion .
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高影响因子
Sustainability
·
2023
·Vol. 15
·期 18
·13382
·
IF 3.9
Qihang Dai,Ju Liu,Junjie Zheng,Bo Fu
Metal–organic frameworks (MOFs) hold great potential for efficient removal of particulate matter (PM) due to their high porosity and tunable surface groups. In this work, sustainable bacterial cellulose (BC) was employed as a substrate for Zr-based MOF (i.e., UiO-66-NH2) deposition. Specifically, the UiO-66-NH2@BC aerogel exhibits a multi-level pore structure with a specific surface area of 103 m2/g. Pristine BC aerogels indicated a removal efficiency of 50.6% for PM2.5, with a pressure drop of 22.8 Pa. While UiO-66-NH2@BC aerogels showed a PM2.5removal efficiency of 96.9%. The filtration mechanism can be assigned to the following reasons: (i) the interception/impaction of PM with the fibrous and porous aerogel network; and (ii) the presence of polar amine groups that boost electrostatic interactions between PM and MOFs. Moreover, as-prepared filters can be regenerated in a facile way with good reusability and long-term stability.
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高影响因子
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
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2015
·
IF 2.128
Xiaoyao Fei,Shaoyun Chen,Chunjie Huang,Dai Liu,Yongchun Zhang
Bovine carbonic anhydrase (BCA) has been immobilized on glycidoxypropyl-functionalized SBA-15 (GFS) for biomimetic carbonation reaction. GFS was characterized by N 2 adsorption–desorption, Fourier transform infrared analysis, 13 C and 29 Si CP MAS NMR spectroscopy. The immobilization time and material dose were optimized. The influences of pH value and temperature on the activity of free and immobilized BCA, storage stability and reusability of immobilized BCA were investigated using a para-nitrophenyl acetate (p-NPA) assay. The kinetic parameters K m and K cat / K m for free and immobilized BCA were found to be 2.4 mM and 896.4 M −1 s −1 , and 3.1 mM and 757.4 M −1 s −1 , respectively. It was observed that the immobilized BCA can retain around 91% of its initial activity up to 30 days at 4 °C, which showed higher storage stability than free BCA. Reusability studies suggested that immobilized BCA could keep high activity after 20 cycles of carbonation reaction. BCA immobilized on GFS (BCA-GFS) were used for hydration of CO 2 . The CO 2 sequestration capacity in terms of conversion CO 2 to calcium carbonate was quantified by organic elemental analysis. The amount of CaCO 3 precipitated over GFS-BCA was nearly the same as that precipitated over free BCA, 227 mg of CaCO 3 /mg of BCA-GFS as compared to CO 2 sequestration capacity of 241 mg of CaCO 3 /mg of BCA.
研究领域:
生物化学
·
环境科学
·
材料科学
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高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 35
·5873-5882
·
IF 4.872
Jiayi Song,Ping Su,Ye Yang,Ting Wang,Yi Yang
The development of new strategies for stabilizing or improving the activities of enzymes has attracted considerable interest because of the wide range of potential applications and high cost of the native enzymes. In this study, a novel trypsin immobilization procedure in which the enzyme was immobilized using polyamidoamine (PAMAM) dendrimer modified magnetic nanoparticles as carriers through DNA-directed immobilization was developed for the first time. The optimal DNA base pairs and the optimal generation of PAMAM on the enzymatic activity were 24 bases and G3.0PAMAM, respectively. The trypsin binding capacity of the immobilized trypsin was 74.6 mg g−1, and the Km and Vmax values were 1.23 mM and 468.35 μmol min−1 mg protein−1, respectively. The immobilized trypsin reactor exhibited excellent reusability and stability properties without significant loss in enzymatic activity. Notably, the high level of enzymatic activity remained at more than 63% after 82 cycles, with only a slight decrease (above 88%) after 14 weeks of continuous use at one-week intervals. The high reversibility and reproducibility of this trypsin dynamic immobilization strategy were also investigated. The significantly improved digestion performance of the immobilized trypsin composites was further demonstrated by digesting cytochrome C, myoglobin and glycated hemoglobin, with sequence coverages of 78%, 99% and 88%, respectively, which were higher than those obtained for the free enzyme. This system therefore shows great potential in high throughput enzymatic assays and proteome analysis.
研究领域:
生物化学
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材料科学
·
蛋白质组学
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DOI
PubMed
高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
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2023
·
IF 3.3
Sun Bingnan,Wang Yajie,Yu Haoran,Zhang Lei,Zhang Weibing
In this work, dextranase was immobilized on the surface of hydroxyapatite nanowires (HAPNWs) through covalent cross-linking method. The prepared HAPNWs-NH 2 -dextranase was characterized by FE-SEM, XRD, TGA and FT-IR spectroscopy. The results show that the dextranase was successfully immobilized on HAPNWs by covalent cross-linking method. The properties of HAPNWs-NH 2 -dextranase were studied and compared with free dextranase, such as catalytic activity, kinetic constants, thermal stability and pH stability. The loading capacity, storage stability and reusability of the immobilized enzymes were investigated. HAPNWs-NH 2 -dextranase demonstrated better enzyme activity at higher temperatures (92.28% vs 33.08% at 70 °C) and higher pH stability (93.17% vs 57.78% at pH 7.0) than free dextranase. The loading capacity of dextranase on HAPNWs-NH 2 -dextranase can reach up to 868.2 mg g −1 . HAPNWs-NH 2 -dextranase retained 83.38% of its initial activity after 12 cycles, indicating that the HAPNWs-NH 2 -dextranase could have great performance in industry fields.
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高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
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2023
·
IF 6.1
Yongkui Li,Xianjin Qi,Guohua Li,Heng Wang,Guizhi Yan,Jiahao Shi
As-containing acidic wastewater from the nonferrous heavy smelting industry is a hazardous As pollution source due to its complex composition, high acidity, and strong toxicity. In this study, an environmentally friendly strategy was proposed to simultaneously remove and immobilize As from acidic wastewater through the synthesis of highly stable scorodite using natural magnetite ore as Fe resource. Firstly, the magnetite ore was dissolved in wastewater to provide a certain amount of starting Fe source for the subsequent arsenic precipitation while creating a suitable pH environment for the synthesis of scorodite. Subsequently, the released Fe ions reacted with As ions in wastewater with undissolved magnetite ore as the nuclear site to form amorphous ferric arsenate, which was then transformed into a well-crystallized scorodite precipitate, thus achieving the As removal and immobilization. Under the optimal reaction conditions, including a Fe/As molar ratio of 2.5, a pre-dissolution time of 5 h, an initial pH of 2.0, a reaction time of 24 h, and a reaction temperature of 90 °C, the residual As concentration in the wastewater rapidly reduced from initial 6259.00 mg/L to 3.76 mg/L, corresponding to an arsenic removal efficiency of 99.94%, and the leaching As concentration in scorodite was 0.42 mg/L. This study provides an efficient and environmentally friendly method for the disposal of high As-containing wastewater from nonferrous smelting.
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2023
·
IF 4.5
Yong Chang,Meiling Liu,Tong Wu,Ruting Lin,Lin Liu,Qijun Song
Most of proteins in the commercial ELISA kits are recombinant with hexahistidine (His 6 ) tags. Based on this fact, we proposed a simple procedure for the preparation of signal labels and the design of competitive immunosensors. The signal labels were fabricated through the high-affinity interaction between the His 6 tails on the surface of recombinant proteins and the coordinatively unsaturated copper ions on the surface of pristine Cu-based metal organic framework (Cu-MOF). The recombinant protein-modified MOF could be captured by the sensor electrode, thus producing a strong differential pulse voltammetry signal through the electrochemical reduction of Cu 2+ ions in MOF. However, the target protein in sample solution could compete with the His 6 -tagged protein on the surface of Cu-MOF to bind the antibody attached on the electrode, thus leading to the decrease in the electrochemical signal. The peak current showed an inversely relationship with the concentration of target protein. The competitive immunosensor exhibited a linear range of 1 pg/mL to 1 ng/mL with SARS-CoV-2 nucleocapsid protein ( N -protein) as the target analyte. We believe that the method can be used to detect other proteins by utilizing the characteristic of recombinant proteins in commercial kits.
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高影响因子
ACS Nano
·
2023
·
IF 17.1
Renjie Tan,Ke Zhang,Yifan Si,Shuai Zhang,Jieqiong Yang,Jinlian Hu
Long-term immobilization of joints can lead to disuse atrophy of the muscles in the joints. Oral nutrients are used clinically for rehabilitation and therapeutic purposes, but bioavailability and targeting are limited. Here, we report tea polyphenols (dietary polyphenols), sustained-release nanofilms that release tea polyphenols through slow local degradation of core–shell nanofibers in muscles. This dietary polyphenol does not require gastrointestinal consumption and multiple doses and can directly remove inflammatory factors and superoxide generated in muscle tissue during joint fixation. The quality of muscles is increased by 30%, and muscle movement function is effectively improved. Although nanofibers need to be implanted into muscles, they can improve bacterial infections after joint surgery. To investigate the biological mechanism of this core–shell nanomembrane prevention, we conducted further transcriptomic studies on muscle, confirming that in addition to achieving antioxidation and anti-inflammation by inhibiting TNF-α and NF-κB signaling pathways, tea polyphenol core–shell nanofibers can also promote muscle formation by activating the p-Akt signaling pathway.
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DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Si-Ke Chen,Da-Wei Pan,Zhuang Liu,Wei Wang,Rui Xie,Xiao-Ting Mu,Xiao-Jie Ju,Liang-Yin Chu
A simple microfluidic strategy is proposed for the controllable preparation of calcium alginate/chitosan (CaA/CS) microcapsules with ultrathin shells for peroxidase immobilization. In this study, the composite microcapsules are composed of CaA and CS, which are beneficial to improve the stability of CaA microcapsules, and are generated using a two-stage capillary microfluidic device. The inner layer of the composite capsule shells is ionically cross-linked CaA, and the outer layer is covalently cross-linked CS. Moreover, the Fe3O4 magnetic nanoparticles are added to facilitate the recovery and reuse of the composite microcapsules. The morphology, permeability, encapsulation efficiency, enzyme activity, and reusability of CaA/CS microcapsules are investigated. The overall thickness of the double-layered shell is about 346 nm. Meanwhile, the microcapsules have good molecular selective permeability, excellent encapsulation performance, stable enzyme activity, and good stability for reusability. The composite microcapsules retained above 80% of residual activity after six recycle uses. The developed novel strategy for fabricating peroxidase-immobilized CaA/CS microcapsules with ultrathin shells is of great potential value for enzyme immobilization applications.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Shiqin Liu,Yuxiang Huang,Qiuqin Lin,Yanglun Yu,Wenji Yu
Wood is a traditional green and renewable environmental-friendly material, but some wood with high polyphenol content is prone to irregular color changes during processing. In this study, ferrous ion (Fe (II)) solution of various mass fractions (0.05%, 0.1%, 0.5%, 1%) was used to immobilize polyphenols in eucalyptus veneers and induce discoloration. With the increase of the Fe (II) concentration, the complexes formed by Fe (II) and polyphenols in the veneers not only enriched the surface, but also began to gather in the cell cavity of wood rays and vessels. The overall color of the veneers became darker and the surface became more hydrophobic with a maximum contact angle of 120°. In addition, the 40-hour xenon lamp aging test showed that the veneer treated with 0.1% Fe (II) had the best light-aging resistance and its color difference was less than 7 and remained stable. Eucalyptus veneers with induced discoloration can not only avoid irregular color changes in subsequent processing, but also meet the differences in people's esthetic perception.
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高影响因子
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