TOXICOLOGY
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2021
·
IF 4.221
Yujie Huang,Jie Zhang,Yizhou Tao,Cheng Ji,Stanley Aniagu,Yan Jiang,Tao Chen
Benzo[a]pyrene (BaP), a prototypical polycyclic aromatic hydrocarbon, is widely present in the environment. BaP-induced heart defects have been frequently reported, but the underlying molecular mechanisms remain elusive. Here, we found that BaP increased heart malformations in zebrafish embryos in a concentration-dependent manner, which were attenuated by supplementation with either CH223191 (CH), an aryl hydrocarbon receptor (AHR) inhibitor, or N-acetyl- l -cysteine (NAC), a reactive oxygen species (ROS) scavenger. While CH and NAC both inhibited BaP-induced ROS generation, NAC had no effect on BaP-induced AHR activation. We further demonstrated that BaP increased mitochondrial ROS, decreased mitochondrial membrane potential, and caused endogenous apoptosis, with all these effects being counteracted by supplementation with either CH or NAC. Resveratrol (RSV), a natural AHR antagonist and ROS scavenger, also counteracted the heart malformations caused by BaP. Further experiments showed that RSV attenuated BaP-induced oxidative stress, mitochondrial damage and apoptosis, but had no significant effect on AHR activation. In conclusion, our findings show that BaP induces oxidative stress via AHR activation, which causes mitochondria-mediated intrinsic apoptosis, resulting in heart malformations in zebrafish embryos, and that RSV had a protective effect against BaP-induced heart defects mainly by inhibiting oxidative stress rather than through antagonism of AHR activity.
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高影响因子
FOOD HYDROCOLLOIDS
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2020
·
IF 7.053
Zheng Fang,Xixi Cai,Jiulin Wu,Lingtuo Zhang,Yapeng Fang,Shaoyun Wang
Plant protein-based nano-delivery systems have rencently drawn increasing attention, and several physical and chemical methods for improving the functional properties of plant proteins have been reported. In this work, we applied ultrasonication and pH-shifting combined treatment on soy protein isolates (SPI) and investigated the structure changes of SPI and its effects on the formation of SPI- sodium alginate (Alg) electrostatic complex and SPI-resveratrol nanocomplex. It was found that the combined ultrasonication/pH-shifting treatment resulted in a smaller particle size and larger surface hydrophobicity, which were 114.6 ± 1.2 nm and 12741 ± 456, respectively. During the formation of the complex with Alg , compared with pH-shifting or ultrasonication treatment, the combined ultrasonication/pH-shifting treatment expanded stable region, where the particle size was smaller and the binding constant with sodium alginate was higher. Moreover, the encapsulation efficiency of resveratrol in SPI increased to 91.4 ± 4.3% after the combined ultrasonication/pH-shifting treatment. Thus, simultaneous treatment combining ultrasonication and pH-shifting was significantly more effective than ultrasonication alone and was thought as an effective method for protein modification.
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高影响因子
Microbial Biotechnology
·
2020
·Vol. 13
·期 4
·1118-1131
·
IF 5.328
Tao Ma,W. Wu,Y. Tu,N. Zhang,Q. Diao
Summary This study aimed to investigate the effect of resveratrol on methane production, rumen fermentation and microbial composition under high-concentrate (HC) and high-forage (HF) diets using the in vitro fermentation system. A total of 25 mg of resveratrol was supplemented into 300 mg of either HC or HF diet. Methane production, total volatile fatty acid (VFA) concentration, molar proportion of VFA, metabolites of resveratrol and prokaryotic community composition were measured after 12 and 24 h of in vitro fermentation. Resveratrol reduced methane production (ml per mg of dry matter degraded) by 41% and 60% under both HC and HF diets ( P < 0.001), respectively, and this result could be associated with the lower abundance of Methanobrevibacter ( P < 0.001) in response to resveratrol. The molar proportion of propionate was significantly higher in the resveratrol group only under the HC diet ( P = 0.045). The relative abundance of 10 bacterial genera was affected by the three-way interaction of treatment, diet and time ( P < 0.05). Resveratrol was partly converted to dihydroresveratrol after 24 h of fermentation, and its degradation could be associated with microbes belonging to the order Coriobacteriales . Our results suggest that multiple factors (e.g. diet and time) should be considered in animal experiments to test the effect of polyphenol or other plant extracts on rumen fermentation, methane emission and microbial composition.
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INDUSTRIAL CROPS AND PRODUCTS
·
2019
·
IF 4.191
Dong-Yang Zhang,Yi Wan,Xiao-Hui Yao,Cong Chen,Yu-Xiong Ju,Fei-Fan Shuang,Yu-Jie Fu,Tao Chen,Wei-Guo Zhao,Li Liu,Long Li
In this study, in order to bio-transform polydatin to resveratrol from Polygonum cuspidatum extract, a 3D-porous structured cellulose microsphere (CMs) immobilized with β-glucosidase acted as a micro-bioreactor. To successfully immobilize β-glucosidase, CMs was treated with epoxidation to attach three different functional amion groups, and finally combined with β-glucosidase. CMs provided large surface area (32.0 m²/g) and many sites for immobilization of β-glucosidase and chemical modification. The porosity of CMs was 90 % and its density was about 1.00 g/m 3 . According to SEM images, the 3D-porous structure was obviously found and the result of FTIR proved the successful modification of amino groups. The crystal structure of CMs was changed from crystalline cellulose I-β to crystalline cellulose II during the sol-gel method. In the biotransformation tests of the Polygonum cuspidatum extracts, the conversion rate of polydatin reached 93.06 %, and after conversion, the concentration of resveratrol was two times higher than the original Polygonum cuspidatum extract. In addition, the cellulose microsphere micro-bioreactor also possessed good reusability, which could maintain 89.2 % activity after being repeatedly operated 8 times. Therefore, this cellulose microsphere micro-bioreactor can be successfully used to produce resveratrol from polydatin, and this micro-bioreactor may also have the potential to convert other plant glycosides into aglycones. Moreover, because of their good biocompatibility, the 3D porous cellulose microspheres can also be used for immobilization of other enzymes to prepare efficient bioreactors.
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高影响因子
Journal of Functional Foods
·
2019
·
IF 3.197
Junlei Liu,Huarong Huang,Zebin Huang,Yuran Ma,Lanyue Zhang,Yan He,Dongli Li,Wenfeng Liu,Susan Goodin,Kun Zhang,Xi Zheng
Eriocitrin is a flavonoid that is isolated from orange peel. Resveratrol is a polyphenol compound, which is present in various fruits, such as grapes. The aim of the present study was to investigate both in vitro and in vivo anti-inflammatory effects of eriocitrin combined with resveratrol using lipopolysaccharide (LPS)-induced RAW264.7 cells and a mouse model of ear edema. The results showed that eriocitrin combined with resveratrol strongly inhibited LPS-induced secretion of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and Interleukin-1β (IL-1β). Moreover, Eriocitrin combined with resveratrol potently inhibited nuclear factor-κB (NF-κB), phosphor-STAT3, and phosphor-AKT, which was accompanied by inhibition of phosphorylation in mitogen-activated protein kinase (MAPK) signaling pathways . Treatment with eriocitrin combined with resveratrol alleviated edema and subcutaneous tissue inflammation caused by 12-O-tetradecanoylphorbol-13-acetate (TPA) in vivo . This study also demonstrated that treatment with eriocitrin and resveratrol decreased the levels of the pro-inflammatory cytokines TNF-α and IL-1β. The results of the present study indicate that eriocitrin combined with resveratrol effectively inhibits inflammatory responses both in vitro and in vivo .
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JOURNAL OF FOOD SCIENCE
·
2019
·Vol. 84
·期 8
·2042-2049
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IF 2.081
Lipeng Han,Qingna Lin,Guoqin Liu,Dongxue Han,Li Niu,Dongxiao Su
It is important to inhibit the food-derived, potentially hazardous chemical glycated lipids by natural products. A model system was established and the products are identified to study the inhibitory mechanism of four types of catechin, resveratrol (RES), and the synthetic antioxidants butylated hydroxylanisole (BHA) and tert-butylhydroquinone (TBHQ) on the formation of carboxymethyl 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (CM-DPPE) by determining hydroxyl radical (OH·), Amadori-1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (Amadori-DPPE) and glyoxal (GO). The results show that the inhibitory rates of catechin and RES on the content of CM-DPPE in the model system are higher than those of BHA and TBHQ. There are at least two inhibitory mechanisms of antioxidants on CM-DPPE. (1) Antioxidants scavenge OH·, which blocks the process of Amadori-DPPE oxidation to form CM-DPPE. (2) Antioxidants trap GO, which blocks the reaction between GO and DPPE to form CM-DPPE. This research will reveal the inhibitory mechanisms of natural antioxidants on glycated lipids from the aspect of scavenging OH· and trapping GO. Practical Application Food manufacturers should pay attention on the production of glycated lipids in food processing. This study will provide the theoretical basis for the use of natural products to inhibit the formation of food-derived glycated lipids. Natural products, such as catechin and resveratrol, can substitute chemical synthesis antioxidants, such as butylated hydroxylanisole and tert-butylhydroquinone, in food processing, which inhibit the formation of glycated lipids.
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高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2019
·Vol. 31
·期 1
·135-145
·
IF 2.162
Danqi Yang,Luming Dong,Xudong Hou,Weikang Zheng,Jian Xiao,Jianzhong Xu,Haiyun Ma
A highly cross-linked poly (cyclotriphosphazene-resveratrol) microsphere (PRV) was synthesized by using hexachlorocyclotriphosphazene (HCCP) and bio-based resveratrol (REV) as raw materials, and the obtained PRV microspheres were applied to improve the flame retardancy and mechanical property of epoxy resin (EP). The TGA results showed that the PRV microsphere is an excellent charring agent and the char yield is as high as 62% at 800°C. The incorporation of PRV makes the initial degradation earlier yet significantly increases the char residue of EP composites. Moreover, the introduction of PRV microspheres into EP greatly promoted the flame retardancy performance. Under 3% of addition of PRV microspheres, the peak heat release rate (PHRR) and total heat release (THR) were decreased by 58.3% and 29.6%, respectively, the limited oxygen index (LOI) value was increased to 29.7% from 25.3% of pure EP. In addition, because of the uniform distribution in EP matrix and the enhancing effect of PRV microspheres, the mechanical properties including tensile modulus of EP composites were strengthened. PRV microspheres in this paper provide a possibility to synthesize a dual functional filler, which acts as both flame retardant and strengthening agent.
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2019
·Vol. 55
·期 5
·1872-1880
·
IF 2.281
Simin Feng,Yuxin Sun,Pei Wang,Peilong Sun,Christos Ritzoulis,Ping Shao
Summary To improve long-term stability of the liposomes, resveratrol (RES) liposomes, epigallocatechin gallate (EGCG) liposomes, RES+EGCG liposomes, RES+EGCG liposomes coated with low methoxyl pectin (LMP) were prepared, and their physical properties were evaluated. The prepared liposomes have a particle sizes of about 110 – 160 nm. The LMP-coated composite liposomes have the largest particle size and zeta potential, the latter indicating higher system stability. Liposome systems were pasteurised and placed in an orange juice system as to study liposome stability in a real the food environment. LMP bridge with metal ions forming a network-like gel in the LMP-coated composite liposomes, thereby enhancing the stability of liposomes. LMP-decorated liposomes could be developed as a formulation for encapsulating and delivering functional ingredients.
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高影响因子
Frontiers in Endocrinology
·
2024
·
IF 3.9
Xiaomin Xu,Donghua Yu,Yu Wang,Xin Jiang,Fang Lu,Shumin Liu
Objective: This study integrates network pharmacology and metabolomics techniques to explore the potential regulatory mechanisms of Res on gouty arthritis (GA).Methods: Network pharmacology was used to predict the mechanism of Res in regulating GA, and methods such as HE staining, ELISA, immunohistochemistry, Real-time PCR, Western blot, and molecular docking were used to verify the role of NF-κB, MAPK, and JAK/STAT inflammatory signaling pathways in the MSU-induced GA rat model. In addition, non-targeted metabolomics techniques were combined to further investigate the mechanism of Res in treating GA.Results: The results of network pharmacology showed that Res may exert its therapeutic effects through the NF-κB signaling pathway. Animal experiments demonstrated that in the MSU-induced GA rat model, pathological damage, serum biochemical indicators, and levels of inflammatory factors were significantly increased, and the NF-κB signaling pathway was activated. The intervention of Res significantly reduced pathological damage, serum biochemical indicators, levels of inflammatory factors, and the activation of NF-κB, MAPK, and JAK/STAT signaling pathways in the model rats. Metabolomics results showed that Res could improve the metabolic trajectory deviations in serum and joint fluid of GA model rats. Through related metabolic pathway analysis, the most affected metabolic pathways were found to be Sphingolipid metabolism, Glycerophospholipid metabolism, Phenylalanine, tyrosine and tryptophan biosynthesis, Pantothenate and CoA, Citrate cycle (TCA cycle), and Arachidonic acid metabolism.Conclusion: Resveratrol can regulate the biosynthetic pathways of arachidonic acid, phenylalanine, tyrosine, and tryptophan, pantothenic acid and CoA biosynthesis pathways, TCA cycle, and other metabolic pathways, thereby regulating the NF-κB, MAPK, and JAK/STAT3 signaling pathways, and inhibiting the acute inflammatory response during GA attacks, showing characteristics of multi-pathway and multi-target action.
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高影响因子
ADVANCED MATERIALS
·
2025
·
IF 26.8
Peng Ye,Yuan Yang,Mengzhe Liu,Jiaqi Meng,Jingyang Zhao,Jiawei Zhao,Jinghui Wang,Qianyun Lu,Jing Liu,Luying Wang,Jiandu Lei,Changlong Wang
Diabetic wounds are a common complication of diabetes and pose a significant threat to human health. High glucose concentration in the wound remains a major obstacle, necessitating effective strategies to achieve sustained glucose consumption for synergistic diabetic wound therapy. In this study, an Au-based nanomaterial is developed that can adjust its morphology in different therapeutic processes. The prepared Au nanowire (ANW) can be converted into Au nanospheres (AS) under ultrasonic conditions by adjusting the amount of polyethylene glycol (PEG) on its surface for convenient delivery. Intriguingly, AS is depolymerized into ANW again in the wound area, prolonging the retention time, and ensuring continuous consumption of glucose. After constructing the morphologically switchable Au nanowire, a polyvinyl alcohol (PVA) is applied it to microneedle and co-delivered it with hemoglobin (Hb)-resveratrol (RES) nanoparticles for synergistic diabetic wound therapy. In a streptozotocin (STZ)-induced diabetic mouse model, the microneedle degraded gradually, and the Hb-RES nanoparticles synergistically ameliorated hypoxia, scavenged ROS, and inhibited macrophage differentiation into pro-inflammatory M1 phenotypes. During this process, ANW continuously catalyzed glucose through its inherent glucose oxidase activity. Thus, this study provides novel insights into the long-term management of glucose concentration during synergistic diabetic wound healing.
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高影响因子
Food Bioscience
·
2024
·
IF 4.8
Jun Yang,Xianxiang Chen,Jieqiong Lin,Mingyue Shen,Yuanxing Wang,Anwesha Sarkar,Huiliang Wen,Jianhua Xie
Herein, a novel oral nano-delivery system (CR-NPs) was designed for co-delivering curcumin and resveratrol to treat ulcerative colitis (UC) using Mesona chinensis polysaccharides and zein via antisolvent precipitation and continuous water evaporation crosslinking. It showed high encapsulation rates for both bioactives due to hydrogen bonding and hydrophobic forces. CR-NPs have favorable in vitro gastrointestinal stability and low pre-colonic release rates, resulting in increased accumulation in the colon. In vivo therapeutic effects of CR-NPs were confirmed through alleviation of UC symptoms and inflammation of UC mice. CR-NPs maintained the balance of intestinal flora and promoted the production of short-chain fatty acids in UC mice. Furthermore, CR-NPs intervention can alleviate the development of UC by modulating arachidonic acid, linoleic acid, and tryptophan metabolism. This study provides some theoretical basis using a novel dietary protocol option that co-delivering natural bioactives to the colon for alleviation of UC.
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高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2024
·
IF 4.7
Zhang Yimiao,Meng Fuliang,Hu Zhenguo,Jia Yufei,Chen Zeyu,Fei Hongwei,Zhou Dapeng,Yuan Xinhua
In this study, resveratrol was substituted for phenol in different molar ratios (4%, 8%, 12%, 16% and 20%) with lignin, phenol and formaldehyde as raw materials, and a new resveratrol modified lignin-based phenolic resin (R-LPF) with high glass transition temperature ( T g ) and improved mechanical properties was synthesized. The curing behaviors of phenolic resin (PF), lignin phenolic resin (LPF) and R-LPF were investigated. The thermal mechanical properties, thermal stability, comprehensive mechanical properties, frictional properties and morphology were systematically tested and analyzed. The results show that the highest T g of the modified R-LPF reaches 346.2 °C. The T s reaches 231.5 °C, and the residual carbon rate reaches 66.2% at 800 °C. For 12% substitution ratio, the maximum tensile strength, bending strength and impact strength are respective 28.72 ± 1.10 MPa, 57.58 ± 1.95 MPa and 3.14 ± 0.19 KJ/m 2 . Compared with PF and LPF, the tensile strength of R-LPF is increased by 21.5% and 40.4%, and the bending strength is increased by 6.5% and 30.4%, while the impact strength is increased by 38.9% and 58.6%, respectively. The wear resistance is also significantly improved. The modified R-LPF structure has good rigidity, high crosslinking density, excellent mechanical and thermal properties, and it makes up for the defects of system performance decline caused by the introduction of lignin and provides a direction for the development of green high-performance phenolic resin.
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危险品化学品经营许可证（带存储）