ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yan Cai,Weifu Ren,Haiyan Wang,Qi Bian
Diabetic kidney disease (DKD) is a common and serious kidney-related complication of diabetic mellitus. Although albuminuria and estimated glomerular filtration rate (eGFR) are commonly used diagnostic biomarkers, they have limitations in the diagnosis of DKD due to the lack of specificity and sensitivity. Urinary N-glycans are emerging as novel biomarkers for predicting renal prognosis in DKD. However, most of the current N-glycan profiling methods for DKD were based on lectin affinity enrichment or hydrophilic interaction chromatography (HILIC) with optical detection, which provided limited N-glycome coverage in low throughput. Herein, we developed a novel N-glycan purification method, termed Ultrafast Glycoprotein Immobilization for Glycan extraction (UltraGIG), for in-depth profiling of urinary N-glycome in DKD using mass spectrometry (MS). In UltraGIG, proteins were rapidly (within 40 min) immobilized to resin with high efficiency (over 98%) through NHS-reactive chemistry , and then N-glycans were enzymatically released from the resin by PNGase F. Owing to good efficiency of immobilization of proteins to resin, the subsequent washing steps to remove proteins and other impurities could be easily performed. The UltraGIG showed good selectivity towards glycans (extracting N-glycans from the mixture of IgG and BSA at a 1:100 M ratio) and samples loss was minimized (detection sensitivity at fmol level). A total of 237 N-glycan compositions were identified from urine samples with DKD and healthy controls, which representing the largest data set of N-glycome from DKD. Compared to healthy controls, 3 N-glycans were up-regulated and 14 N-glycans were down-regulated in DKD patients. Collectively, UltraGIG offers a competitive sample purification method for in-depth analysis of urinary N-glycome by MS and provide new insights into biomarker study for the diagnosis of DKD.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Lei Wang,Wenxuan Li,Wenjing Zhi,Dandan Ye,Wenli Zhang,Liang Ni
By employing the oxidation property of hypochlorite (OCl − ), a new probe based on coumarin dye and an OCl − -specific reactive moiety of hydrazide group was designed and synthesized. The probe rendered turn-on type fluorogenic response toward OCl − via hypochlorite-triggered oxidative hydrolysis of hydrazide to release strongly fluorescent coumarin. Most notably, the probe showed sufficiently satisfactory selectivity to OCl − over other oxidants, and high sensitivity with approximate 18-fold fluorescence enhancement at nanomolar level. Moreover, the fast detection of OCl − could be realized by the probe with only 30 s. The interaction mechanism has been proposed and further confirmed by the changes in electrospray ionization (ESI) mass spectra. The probe was also successfully applied to fluorescence imaging of OCl − in its vitro macrophage cells.
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DYES AND PIGMENTS
·
2020
·
IF 4.613
He Li,Donglin Li,Bowen Qin,Wenliang Li,Haoyan Zheng,Xiaoying Zhang,Jingping Zhang
An extremely stable pillar-layered metal-organic framework (MOF), [Cd 4 (NDC) 3 (4-Hptz) 2 (H 2 O) 2 ]·H 2 O (1), has been successfully designed and synthesized (4-Hptz = 5-(4-pyridyl)-tetrazolate, NDC = 1,4-Naphthalenedicarboxylic acid). Compound 1 can detect Cr 3+ in water by fluorescence enhancement, which is the first known case of MOF. This is of great significance for our study on new fluorescent probes .
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Forensic Toxicology
·
2016
·Vol. 34
·期 2
·398-402
·
IF 0
Shintani-Ishida Kaori,Kakiuchi Yasuhiro,Ikegaya Hiroshi
The toxicological detection of the new synthetic cathinone 4′-methyl-α-pyrrolidinohexanophenone (MPHP) in urine samples has been impossible, because much of MPHP is metabolized before its excretion into urine. In this study, we successfully quantified unmetabolized MPHP in urine of an autopsy case using a sensitive method by liquid chromatography–time-of-flight-mass spectrometry. The quantification method showed good linearity in the range of 1.00–100 ng/mL, and the limit of detection was 0.5 ng/mL in human urine. In the autopsy case, the concentrations of MPHP in urine, plasma, and liver tissue samples were determined to be 60.1, 32.9 ng/mL, and 63.1 ng/g, respectively.
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MACROMOLECULES
·
2023
·
IF 5.5
Anhong Liu,Xiaodong Ye
Mixed micelle systems have gained significant attention due to their ability to integrate multifunctionality based on different copolymers. However, an understanding of chain exchange behavior in mixed micelles is limited. In this study, we reported a mixed micelle system where noncovalent cross-linking between the corona blocks enhanced micelle stability. Specifically, we utilized polystyrene-block-poly(2-ethyl-2-oxazoline) (PS-b-PEtOx) and polystyrene-block-poly(methacrylic acid) (PS-b-PMAA) to form the mixed micelle system. Donor- and acceptor-labeled PS-b-PEtOx was employed to investigate the chain exchange kinetics using fluorescence resonance energy transfer (FRET). The addition of PS-b-PMAA facilitated the formation of hydrogen bonds between EtOx and protonated MAA units, resulting in a noncovalent cross-linked corona. As the pH decreases, the percentage of affected PS-b-PEtOx gradually increases, while the extra energy barrier first decreases and then increases. This is mainly due to the conformational changes of PMAA chains, which cause crowding between the core and shell, and the interactions between PMAA and PEtOx, especially hydrogen bonding. This study demonstrates that the hydrogen-bonded cross-linking of the corona in the mixed micelle system can effectively control the chain exchange rate and improve micelle stability.
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PLANT DISEASE
·
2023
·Vol. 107
·期 11
·
IF 4.5
Qiuyan Bi,Fen Lu,Jie Wu,Xiuying Han,Wenqiao Wang,Jianjiang Zhao
Pear powdery mildew (PPM), caused by Phyllactinia pyri , is one of the most serious diseases affecting production in the Hebei pear-growing region of China. Iminoctadine trialbesilate and trifloxystrobin are known to have broad-spectrum activity against a wide range of plant pathogens, including P. pyri . A total of 105 P. pyri strains were isolated from 11 cities in Hebei Province from 2017 to 2019. Iminoctadine trialbesilate and trifloxystrobin significantly inhibited P. pyri growth. Microscopic observation showed that P. pyri mycelia had different degrees of desiccation and that the conidial cell contents had been released. The sensitivities of 60 P. pyri strains to iminoctadine trialbesilate and trifloxystrobin were determined in vitro, and the average EC 50 values were 0.5773 ± 0.0014 and 1.2038 ± 0.0010 μg/ml, respectively. The average EC 50 values for 85 and 75% of the strains with continuous single peak frequency distributions were 0.4534 ± 0.0012 and 0.8124 ± 0.0039 μg/ml, respectively. These data could be used as the baseline sensitivities of P. pyri to these two fungicides. The maximum difference multiples of the sensitivities of P. pyri strains from the different cities to iminoctadine trialbesilate and trifloxystrobin were 13.5- and 17.2-fold, respectively. Cluster analysis showed that there was no significant correlation between P. pyri sensitivity and geographical origin. The field efficacies in controlling PPM were higher than 85%. These findings can improve how we monitor iminoctadine trialbesilate and trifloxystrobin resistance and improve application efficiency.
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JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 24
·4857-4864
·
IF 2.557
Zhongping Huang,Yueting Xu,Yilei Huang,Charles Liu,Kezhi Jiang,Lili Wang
A novel method based on direct analysis in real time integrated with mass spectrometry was established and applied into rapid determination of ginkgolic acids in Ginkgo biloba kernels and leaves. Instrument parameter settings were optimized to obtain the sensitive and accurate determination of ginkgolic acids. At the sample introduction speed of 0.2 mm/s, high intensity of [M–H] – ions for ginkgolic acids were observed in the negative ion mode by utilization of high-purity helium gas at 450°C. Two microliters of methanol extract of G. biloba kernels or leaves dropped on the surface of Quick-Strip module was analyzed after solvent evaporated to dryness. A series of standard solutions of ginkgolic acid 13:0 in the range of 2–50 mg/L were analyzed with a correlation coefficient r = 0.9981 and relative standard deviation ( n = 5) from 12.5 to 13.7%. The limit of detection was 0.5 mg/L. The results of direct analysis in real time-mass spectrometry were in agreement with those observed by thermochemolysis gas chromatography. The proposed method demonstrated significant potential in the application of the high-throughput screening and rapid analysis for ginkgolic acids in dietary supplements.
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Journal of Materials Chemistry A
·
2018
·Vol. 6
·期 8
·3543-3551
·
IF 9.931
W. Zheng,P. Zhang,J. Chen,W. B. Tian,Y. M. Zhang,Z. M. Sun
To stabilize the layer structures of MXenes, carbon nanotubes (CNTs) have been successfully grown in situ by a facile microwave irradiation method under ambient conditions. The synthesized composites based on various MXenes including Ti3C2, Ti2C and V2C exhibited hybrid structures with uniformly distributed multi-walled CNTs anchored on the layer structured MXene networks. Benefiting from the unique structural features, these composites exhibit excellent electrochemical properties as anode materials in lithium ion batteries. The CNTs@Ti3C2 composite exhibits high reversible capacities of 430 mA h g−1 at 1 A g−1 and 175 mA h g−1 at 10 A g−1 (31.25 C), and is not only better than the pristine Ti3C2 (99 mA h g−1 and 45 mA h g−1, respectively), but also superior to the recently reported MXene composites. The impressive electrochemical performance is attributed to the synergetic effects of the connective CNT bridges, large-capacity metal/metal oxides and the fine conductive MXene matrix. This in situ growth strategy of CNTs shows great potential in modulating the structure and properties of nanomaterials for advanced applications.
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BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Fan Cheng,Hui Zhang,Siyu Sun,Zhengkui Li
A two-chamber microbial photoelectrochemical cell (MPEC) with a denitrification biocathode and an α-Fe 2 O 3 /P3HT photoanode was established aimed to enhance nitrate removal from wastewater of low carbon sources and achieve simultaneous ibuprofen degradation. The results demonstrated that the average removal of NO 3 – -N in the biocathode reached 96.56 ± 0.72% when the COD/NO 3 – -N was 0.75, and the relative contributions of heterotrophic and autotrophic denitrification were 21.47% and 78.53%, respectively. When there was no organic source in the influent, the maximum removal of NO 3 – -N was less than 45%. High-throughput sequencing revealed that both heterotrophic bacteria, such as Bacillus , and autotrophic bacteria (e.g., Thermomonas and Hydrogenophaga ) dominated in the cooperative denitrification biocathode. Moreover, the functional gene analysis showed that the abundance of genes related to denitrification was highest in the cooperative denitrification biocathode. In addition, the photocatalytic degradation efficiency of ibuprofen in the anode chamber attained 67.95% ± 0.97%.
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CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Yu-Heng Mao,Yi-Xuan Xu,Yan-Heng Li,Jing Cao,Feng-Ling Song,Dan Zhao,Yimin Zhao,Zhao-Mei Wang,Yan Yang
This study investigated the effect of konjac glucomannan (KGM) of different molecular weight on fecal microflora against antibiotic disturbance. KGM (~1.8 × 10 7 Da) was partially hydrolysed with trifluoroacetic acid (TFA) for 10 and 60 min to KGM1 (~2.1 × 10 4 Da) and KGM2 (7413 Da), respectively. The acid treatment caused significant reduction of intrinsic viscosity , average molecular weight (MW) and particle size of KGM, but brought limited change to the molecular structure. Low-MW KGM2 showed the most significant effect on fecal microflora in the presence of two common antibiotics (ampicillin and clindamycin), by increasing the relative abundance of Bifidobacteriaceae while decreasing the proportion of Enterobacteriaceae . Additionally, both the native and acid-treated KGM counteracted the adverse influence of antibiotics on the production of short chain fatty acids . The results have demonstrated the effect of KGM on gut microbiota with antibiotic disturbance.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2019
·
IF 14.229
Qianqian Huang,Yun Hu,Yun Pei,Jinhui Zhang,Mingli Fu
TiO 2 @NH 2 -MIL-125, composite photocatalysts with close interfacial contact between NH 2 -MIL-125 and TiO 2 , were successfully synthesized via an in-situ solvothermal method for formaldehyde (HCHO) removal under UV irradiation. Samples were characterized by using a variety of analytical techniques. The results showed that TiO 2 particles were highly dispersed on the surface of NH 2 -MIL-125, and there was a strong electronic interaction (Ti-N-C) between elements of the composite. TiO 2 @NH 2 -MIL-125 exhibited significantly enhanced photocatalytic performance for HCHO removal compared to pure TiO 2 and NH 2 -MIL-125 that was ascribed to the synergistic combination of high adsorption capacity of NH 2 -MIL-125, high dispersion of TiO 2 and efficient interfacial charge transfer between TiO 2 and NH 2 -MIL-125. Furthermore, the HCHO removal rate over the composite remained at 90% after 122 h of continuous photocatalytic reaction, indicating the high stability of the catalyst. This work provides new insights that will be helpful in the further development of photocatalysts for indoor air purification.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Bingzhi Liu,Haitao Lu,Shenyu Wu,Zizeng Wang,Li Feng,Huaili Zheng
Conventional flocculants are difficult to effectively remove dissolved ions and organic matter from water. This work aims to develop an environmentally friendly and renewable core–shell type flocculant (MCHS-g-P(AM-IA)) with octopus tentacle-like strong adsorption structure. The organic shell hydrolyses into molecular chains like octopus tentacles and adsorb dissolved ions/organic macromolecules through suckers-like functional groups, while the magnetic core acts as the growth center of flocs. Configuration and physical/chemical properties of the flocculant were analyzed by a series of characterization methods. Set Cu(II) and malachite green (MG) as models of heavy metal and organic pollutants separately, the removal capacity of MCHS-g-P(AM-IA) for water contaminants was studied in single-contaminant system and binary-contaminant system. The flocculation behavior and mechanism of MCHS-g-P(AM-IA) were deeply investigated by analysis of pH-dependence, background cationic influence and flocculation kinetics and SEM-EDS/XPS analysis of flocs. Furthermore, the flocculation performance in actual water matrix and regeneration tests of MCHS-g-P(AM-IA) were carried out to evaluate the applicable feasibility of flocculants for purification of complex wastewater in single stage water treatment.
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