SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Ruodong Huang,Jin Quan,Baochang Su,Chunzong Cai,Shuhua Cai,Yaowei Chen,Zongxia Mou,Ping Zhou,Dong Ma,Xin Cui
Monitoring C-reactive protein (CRP) levels in point-of-care testing (POCT) scenarios is of great value in early diagnosis, prevention and treatment of inflammation in patient’s home, development country as well as other resource-limited settings. Here, we reported a sensitive, economic and simple biosensing approach for visual and sensitive CRP detection by trapping and accumulating microparticles in a photolithography-free, low-cost microfluidic chip. A competition assay among microparticles, magnetic nanoparticles and CRP antigen is utilized to quantify the CRP concentrations. When CRP antigen is present, fewer magnetic nanoparticles can bind to antibody-conjugated microparticles, leaving the free microparticles flowing and trapping in a microfluidic particle accumulation chip after magnetic separation, and forming a length-quantifiable visual bar, which is proportional to the concentrations of CRP in detection samples. Therefore, the target protein can be quantitatively detected by reading the length-quantifiable visual bar in a microfluidic channel by the naked eyes. Of note, a novel two-step competition assay was utilized to achieve the highly sensitive and specific CRP detection with a limit of detection (LOD) of ~ 32 pg/mL, and a wide detection range from 100 pg/mL to 10 µg/mL. The developed method also showed high selectivity , good accuracy and great stability to different temperature and incubation time. More importantly, the design of the sensor could be applicable for detecting any other biomarkers feasibly, thus providing a universal platform for visual, sensitive and economic clinical biomarker measurement by the naked eyes for POCT applications without any auxiliary equipment .
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yifei Li,Jingwen Li,Ya Cao,Ping Jiang,Yuguo Tang,Zutao Chen,Kun Han
Sensitive and specific assay of hepatitis C virus (HCV) RNA has a great clinical significance for the diagnosis of hepatitis C in window period. A colorimetric assay is described for determination of the RNA of hepatitis C virus. The method is based on the in situ synthesis of graphene quantum dot-silver nano-composites (GQD/Ag NCs), magnetic separation and catalyzed hairpin assembly reaction (CHA). The silver component of GQD/Ag NCs react with H 2 O 2 generated by glucose oxidase , which are induced via CHA. Then the light-yellow solution turns into colorless transparent in the presence of the target RNAs. The method has a linear response in the 25–500 pM RNA concentration range, and the detection limit is 24.84 pM. The method is simple, stable and sensitive and might be extend to the assay of other RNA viruses . The retro-transcription free and visual distinguishable properties endow this strategy a promising application in the screening of HCV.
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高影响因子
CHEMICAL PAPERS
·
2022
·Vol. 76
·期 6
·3937-3947
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IF 2.146
Li Xinxin,Lin Min,Zhang Hongfeng,Ji Wenquan,Shi Yuhan,Qi Zhiyang,Fu Tianwen,Li Qinran,Deng Qiliang
A kind of surface imprinted particles was synthesized to recognize cytochrome c (Cyt c) by a novel strategy combining reversible addition-fragmentation chain transfer (RAFT) radical polymerization and epitope surface imprinting. N-terminal epitope nonapeptide of Cyt c was chosen as the template. The selectivity of these particles for protein recognition was obviously improved due to the selection of epitopes rather than the whole protein as templates and the controllable length of polymer shell chain. After epitope had been modified on the surface of silica, the surface imprinted polymer with controlled imprinted layer was synthesized with monomers and cross-linkers by RAFT radical polymerization strategy. The epitope surface imprinted particles were obtained after the peptide removed and trithioester group destruction by hexylamine. The binding capacity of N-terminal peptide reached 1.88 mg peptide per gram with imprinting factor (IF) as 2.42 while the binding capacity of Cyt c reached 8.89 mg protein per gram with IF as 1.71, which were obviously higher than the IF of the material without RAFT strategy. Moreover, Cyt c could be selectively recognized by the epitope surface imprinted particles even in presence of the competitive proteins with different molecular weight and isoelectric point. The performance of protein recognition remained 90% after five cycles of adsorption and desorption. All these results demonstrated that the epitope surface imprinted particles prepared by RAFT strategy are promising to achieve the protein recognition with higher recognition ability, selectivity and reusability.
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ChemElectroChem
·
2022
·Vol. 9
·期 5
·e202101381
·
IF 4.782
Ru Meng,Feng Li,Dan Li,Baokang Jin
Graphical Benzo[c]cinnolines , an important framework material in the field of pharmaceutical chemistry, were synthesized efficiently and conveniently with CO 2 as deoxidizer by a green electrochemical method, sealing CO 2 . Benzo[c]cinnolines are an essential framework material in medicinal chemistry owing to its promising anti-cancer and antioxidant activity, and good pharmacological properties. Accordingly, benzo[c]cinnolines are synthesized through electrochemical reduction of 2,2’-dinitrobiphenyl (DNBP) in aprotic solvents with CO 2 . Cyclic voltammetry (CV), cyclic voltabsorptometry (CVA), derivative cyclic voltabsorptometry (DCVA) techniques, and in-situ FT-IR spectroelectrochemistry are used to investigate the mechanism of electrochemical reduction of DNBP in the presence of CO 2 . It is a reversible 2-electron-transfer reaction in an aprotic solution of DMSO in the absence of CO 2 , while it turned into a completely irreversible 8-electron process in the presence of CO 2 . Benzo[c]cinnolines were finally obtained by potentiostatic electrolysis with conversion rate of 99 %, indicating the high selectivity of this reaction. Above all, DNBP can be electrochemically reduced into BC in the presence of CO 2 , providing a theoretical and practical path for electrochemical synthesis of bioactive benzo[c]cinnolines (BC).
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Nanomaterials
·
2021
·Vol. 12
·期 1
·139
·
IF 5.076
Shuqing Chen,Qi Ding,Yan Gu,Xin Quan,Ying Ma,Yulong Jia,Hongmei Xie,Jinzhu Tang
The tribological performances of fullerenol and nanodiamonds (NDs) as additives in water-based lubricants for amorphous carbon (a-C) coatings are investigated to avoid disadvantage factors, such as chemical reactions and deformation of particles. The effects of size and additive amount on tribological properties of nanoparticles are studied by rigid nanoparticles within the dot size range. The results show that owing to its small particle size (1–2 nm), fullerenol cannot prevent direct contact of the friction pair at low concentration conditions. Only when the quantity of fullerenol increased to support the asperity contact loads in sufficient concentration did nano-bearings perform well in anti-friction and anti-wear effects. Unlike fullerenol, nanodiamond particles with a diameter of about 5–10 nm show friction-reducing effect based on the nano-bearing effects at ultra-low concentration (0.01 wt.%), whereas particles at higher concentration block the rolling movement, hence increasing the coefficient of friction (COF) and wear. As a result of the effect of difference in size, fullerenol provides a better overall lubrication, but it is hard to reach a friction coefficient as low as NDs even under the optimal conditions.Keywords:fullerenol;nanodiamonds;water-based lubricant additive;a-C;nano-bearing effects
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Materials Science and Engineering B-Advanced Functional Solid-State Materials
·
2023
·
IF 3.6
Kezhong Wu,Feifei Nie,Xi Qi,Yingshan Wu,Hui Zhao,Mingxing Wu
Vanadium-based nitrides have been applied as Pt-free counter electrodes (CEs) electro-catalysts for dye-sensitized solar cells (DSSCs) due to inexpensive cost and easy doping and modification. Herein, polyoxovanadate (NH 4 ) 2 V 6 O 16 assisted by melamine to prepare VN@C composites by five different synthesis routes, including solvothermal, sol–gel, hydrothermal, ball milling, and chemical mixing method. And then, five VN@C composites were assembled as CEs in the encapsulated DSSCs, and yielded power conversion efficiencies of 4.47, 5.04, 5.35, 5.49 and 6.11% for regenerate traditional I 3 − /I − shuttles by the photocurrent-photovoltage ( J - V ) test, respectively. The catalytic performances of VN@C composites CEs were assessed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and Tafel polarization experiments, and the significant effects of different synthetic routes were confirmed. The results revealed that VN@C composite CEs prepared by chemical mixing method had higher catalytic performance in DSSCs, which is due to the improvement of conductivity and the high concentration of active sites.
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TALANTA
·
2023
·
IF 6.1
Chen Cui,Xinyao Lin,Jie Lv,Hang Guo,Lu Shen,Guoqiang Xiang,Wenjie Zhao,Dechen Jiang
Designing innovative electrochemiluminescence (ECL) immunosensors is critical for the detection of biomarkers with a low concentration and the precise evaluation of clinical diseases. Herein, a Cu 3 (hexahydroxytriphenylene) 2 (Cu 3 (HHTP) 2 ) nanoflake-based sandwich-type ECL immunosensor was constructed for C-Reactive Protein (CRP) detection. The Cu 3 (HHTP) 2 nanoflake , an electronically conductive metal–organic framework (MOF), has a periodically arranged porous structure with a cavity size of 2 nm, which not only accommodates a large amount of Ru(bpy) 3 2+ but also confines the spatial diffusion of active species. Therefore, the Ru(bpy) 3 2+ -loaded Cu 3 (HHTP) 2 nanocomplex (Ru@CuMOF) as an ECL emitter exhibits an enhanced ECL efficiency. The ECL resonance energy transfer (ECL-RET) was accomplished by combining Ru@CuMOF used as a donor with gold nanoparticles-functionalized graphene oxide nanosheets (GO-Au) utilized as an acceptor. This should be ascribed to the fact that the ECL emission spectrum of Ru@CuMOF shows the strongest signal intensity at 615 nm, overlapping with the absorption spectrum of GO-Au at 580–680 nm. Targeted detection of CRP in human serum samples was achieved by the sandwich-type immunosensor based on the ECL-RET mechanism with a 0.26 pg mL −1 detection limit. The electro-activated hybrids of Cu 3 (HHTP) 2 and ECL emitters provide a new sensing strategy for the high-sensitivity detection of disease markers.
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SOLAR ENERGY MATERIALS AND SOLAR CELLS
·
2023
·
IF 6.9
Changjun Wu,Hong Chen,Jingru Tan,Danna Zhou,Haoshen Liang,Shijie Zhen,Hao Liu,Yuehui Wang,Kaiwen Lin
In pursuit of high-performance electrochromic conjugated polymers (ECPs), three benzotriazole-thiophene ECPs employing benzotriazole as acceptor and different thiophene repeating units as donors, namely, PBTz-T, PBTz-BT, and PBTz-TT, were synthesized via direct (hetero)arylation polymerization (DHAP). Meanwhile, following the above ECPs model, we investigated the effect of corresponding conjugated chain length of monomers on their optical, electrochemical and electrochromic properties. The results showed that along with the increase of the numbers of thiophene in the repeating unit of ECPs, the electronic and optical band gaps decreased, the UV–Vis absorption and fluorescence spectra showed significant bathochromic shift, and the electrochemical cycle stability gradually improved. PBTz-T exhibited giant coloration efficiency (916 cm 2 C −1 at 530 nm), which is one of the best results in the visible region. And higher coloration efficiency means less energy consumption, which holds great potential for various applications, including electrochromic back covers of smartphones. PBTz-BT displayed the highest transmittance (28% at 550 nm), and BTz-TT illustrated the fastest switching time (0.6 s/0.5 s for doping/dedoping process). Flexible electrochromic device (ECD) fabricated employed PBTz-T as functional layer gradually changed from red (reduction) to dark green (oxidation), and maintained good cycling stability.
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ACS Applied Nano Materials
·
2023
·
IF 5.9
Zongwei Guo,Zhao Wang,Jiajun Ma,Lin Jin,Yuhui Ao
Introducing two-dimensional (2D) nanoreinforced materials on the surface of carbon fibers (CFs) is a promising strategy for overcoming the chemical inertness of CFs. However, disordered alignment and uneven dispersion of 2D nanoreinforced materials on the CF surface are the most critical issues that must be addressed. Herein, MXene (M) was functionalized using graphene oxide (G) to produce intercalated 2D reinforcement materials, and robust Ti–O–C covalent bonds were introduced to ensure that M and G have strong binding forces. An interface layer with a more ordered and compact “brick-and-mortar” structure was developed on the CF surface to obtain CF composites with high strength and toughness. Owing to the structure superiority, robust Ti–O–C covalent bonding, and abundant π–π interactions, the optimal CF composites achieved high strength and toughness, with a tensile strength of 1587 MPa and a toughness of 45 MJ m–3. Meanwhile, the interlaminar shear and flexural strengths increased to 82 and 1178 MPa, respectively. The reinforcing mechanisms of the unique interface layer on CF composites were systematically investigated via data analysis and modeling. This study provides a constructive design inspiration for fabricating high-performance CF-reinforced polymer composites, considerably expanding their applications.
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Molecular Catalysis
·
2023
·
IF 4.6
Mengyu Dou,Yan Qiao,Xu Hu,Hengyang Miao,Ligong Zhou,Xuekuan Li,Xianglin Hou,Yingxiong Wang,Mingxing Tang
The poor textural properties of initial biochar materials have hindered their extensively applied as catalyst supports. Herein, cellulose biochar materials with the gradient porous and high specific surface area were synthesized by adding different ratios of ZnCl 2 , and as supports to prepare Pd/C cel catalysts for efficient maleic acid hydrogenation in water. The characterization results indicated that the textural propertiesand dispersion of Pd in Pd/C cel catalysts depended on the ratio of ZnCl 2 to cellulose. The Pd/C cel ZnCl 2(1:3) catalyst exhibited the best catalytic activity among all catalysts and outstanding stability for reusing 8 times under mild conditions (60 °C, 2 MPa). In addition, this synthesis strategy was also suitable for other biomass materials (glucose, sucrose, and starch) and the catalytic performance of ZnCl 2 -modified Pd/C catalysts in maleic acid hydrogenation reaction also improved dramatically. The carbonization mechanism was discussed by TG-MS and the results illustrated that ZnCl 2 reduced the carbonization temperature and altered the pyrolysis behavior of biochar as a result of excellent catalytic behavior. This work provided a facile and universal strategy for synthesizing biochar high-efficient supported catalysts with gradient porous structure and high specific surface area.
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Small
·
2021
·Vol. 18
·期 7
·2106355
·
IF 13.281
Shuting Huang,Yanxian Geng,Jie Xia,Dongyun Chen,Jianmei Lu
The cathode material properties of the microbial fuel cell (MFC) have a quite important effect on their power generation capacity. Excellent oxygen reduction reaction (ORR) performance is the key to obtaining the remarkable capability of MFC. In this study, a series of catalysts are successfully prepared by a simple step-by-step hydrothermal, in situ growth, solution polymerization, and pyrolysis procedure. Here, the NiCo nanoparticles loading on nitrogen/carbon dual-doped matrix annealing at 800 °C (NiCo@DNC-800) under Ar shows good ORR activity with a maximum power density of 2325.60 ± 41.96 mW m −2 in the case of the 2 mg cm −2 minimal catalyst loading, and which is about 2.16 times more than that achieved by 20% Pt/C (1074.21 ± 39.36 mW m −2 ). The unique N/C duel-doped matrix provides more graphitic-N and pyridinic-N that can reduce the resistance of electron diffusion and transport, together with the synergistic catalysis of NiCo active sites improving the oxygen reduction reaction performance of MFC greatly. In addition, the NiCo@DNC-800 cathode catalyst demonstrates that composite materials have great application potential in water pollution treatment and new green energy strategies.
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Journal of Energy Chemistry
·
2023
·
IF 13.1
Katam Srinivas,Zhuo Chen,Anran Chen,Fei Ma,Ming-qiang Zhu,Yuanfu Chen
The development of efficient single-atom catalysts (SACs) for the oxygen reduction reaction (ORR) remains a formidable challenge, primarily due to the symmetric charge distribution of metal single-atom sites (M-N 4 ). To address such issue, herein, Fe-N x sites coupled synergistic catalysts fabrication strategy is presented to break the uniform electronic distribution, thus enhancing the intrinsic catalytic activity. Precisely, atomically dispersed Fe-N x sites supported on N/S-doped mesoporous carbon (NSC) coupled with FeS@C core-shell nanoparticles (FAS-NSC@950) is synthesized by a facile hydrothermal reaction and subsequent pyrolysis. Due to the presence of an in situ-grown conductive graphitic layer (shell), the FeS nanoparticles (core) effectively adjust the electronic structure of single-atom Fe sites and facilitate the ORR kinetics via short/long-range coupling interactions. Consequently, FAS-NSC@950 displays a more positive half-wave potential ( E 1/2 ) of 0.871 V with a significantly boosted ORR kinetics (Tafel slope = 52.2 mV dec −1 ), outpacing the commercial Pt/C ( E 1/2 = 0.84 V and Tafel slope = 54.6 mV dec −1 ). As a bifunctional electrocatalyst, it displays a smaller bifunctional activity parameter (Δ E ) of 0.673 V, surpassing the Pt/C-RuO 2 combination (Δ E = 0.724 V). Besides, the FAS-NSC@950-based zinc-air battery (ZAB) displays superior power density, specific capacity, and long-term cycling performance to the Pt/C-Ir/C-based ZAB. This work significantly contributes to the field by offering a promising strategy to enhance the catalytic activity of SACs for ORR, with potential implications for energy conversion and storage technologies.
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