BMC Medicine
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2025
·Vol. 23
·期 1
·1-16
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IF 8.3
Li Yinyan,Yang Sizu,Jiang Feng,Luo Siqi,Liang Jinlong,Jiang Linrui,Chen Zhixuan,Chen Xin,Yang Jie
Background Influenza A virus (IAV) is a major cause of seasonal and global pandemics, posing serious health risks. Repositioning approved drugs offers an efficient antiviral strategy, particularly as calcium (Ca 2 ⁺) is crucial for IAV infection, making Ca 2 ⁺ channel blockers (CCBs) promising candidates for antiviral agents. Methods The in vitro antiviral activity of cilnidipine was evaluated using MTT assays, qRT-PCR, plaque assays, and western blotting. Mechanistic studies involved time-of-addition, viral internalization, pseudovirus neutralization, and HA (hemagglutinin) syncytium assays. For in vivo analysis, BALB/c mice were intranasally infected to evaluate the effects of cilnidipine on viral titer, lung index, pulmonary inflammatory mediators, and survival rate. Results In vitro, cilnidipine exhibits antiviral activity against IAV during the early stages of infection. It disrupts clathrin- and caveolin-mediated endocytosis to inhibit the internalization of IAV and interacts with the viral HA2 subunit to impede virus membrane fusion. Additionally, cilnidipine suppresses the PI3K-AKT and p38 MAPK pathways activated by IAV infections. In vivo, cilnidipine reduces virus titers and lung index, ameliorates lung pathology, and inhibits pulmonary inflammatory mediator expression, improving survival rates. Conclusions These findings highlight the promising anti-IAV properties of cilnidipine both in vitro and in vivo, suggesting its potential as a clinical agent for emergencies against influenza outbreaks.
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Antioxidants
·
2024
·Vol. 13
·期 6
·684
·
IF 6
Siqi Chen,Yajing Li,Enchan Wu,Qing Li,Lan Xiang,Jianhua Qi
Aging is often accompanied by irreversible decline in body function, which causes a large number of age-related diseases and brings a huge economic burden to society and families. Many traditional Chinese medicines have been known to extend lifespan, but it has still been a challenge to isolate a single active molecule from them and verify the mechanism of anti-aging action. Drugs that inhibit senescence-associated secretory phenotypes (SASPs) are called “senomorphics”. In this study, arctigenin (ATG), a senomorphic, was screened from the Chinese medicineFructus arctiiusing K6001 yeast replicative lifespan. Autophagy, oxidative stress, and telomerase activity are key mechanisms related to aging. We found that ATG may act through multiple mechanisms to become an effective anti-aging molecule. In exploring the effect of ATG on autophagy, it was clearly observed that ATG significantly enhanced autophagy in yeast. We further verified that ATG can enhance autophagy by targeting protein phosphatase 2A (PP2A), leading to an increased lifespan. Meanwhile, we evaluated the antioxidant capacity of ATG and found that ATG increased the activities of the antioxidant enzymes, thereby reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels to improve the survival of yeast under oxidative stress. In addition, ATG was able to increase telomerase activity by enhancing the expression ofEST1,EST2, andEST3genes in yeast. In conclusion, ATG exerts anti-aging effects through induction of autophagy, antioxidative stress, and enhancement of telomerase activity in yeast, which is recognized as a potential molecule with promising anti-aging effects, deserving in-depth research in the future.
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JOURNAL OF ETHNOPHARMACOLOGY
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2024
·
IF 4.8
Jiamin Ye,Jiangyu Yao,Shaojing Xu,Guyu Xiao,Yuwei Jia,Ningjun Xie,Jizhong Yan,Xuhui Ying,Hui Zhang
Ethnopharmacological relevance Fufang Qiling granules (FQG), derived from the traditional Qiling Decoction with a longstanding clinical history, is utilized for the treatment of hyperuricemia (HUA). FQG is formulated with a combination of seven Chinese herbs based on the principles of traditional Chinese medicine (TCM) theories. Clinical evidence indicates that FQG exhibits favorable therapeutic effects in reducing uric acid (UA) levels and attenuating renal damage. Aim of this study To elucidate the potential active components and pharmacological mechanism of FQG in the treatment of HUA, and to provide an experimental basis for the development of efficient and low-toxicity TCM for HUA treatment. Materials and methods A HUA rat model induced by potassium oxonate and adenine was established to initially evaluate the hypouricemic effects of FQG. Chemical analyses were conducted using an ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Network pharmacology was used to investigate the active components and mechanism of FQG in the treatment of HUA. Potential Xanthine oxidase (XOD) inhibitors were screened from FQG based on ultrafiltration liquid chromatography and mass spectrometry (UF-LC-MS). Molecular docking, surface plasmon resonance (SPR) and circular dichroism (CD) spectroscopy were applied to validate the interactions between the active components and XOD. Results In comparison to the model group, treatment with FQG significantly decreased serum UA, serum creatinine (CREA), serum blood urea nitrogen (BUN), and liver XOD activity. Additionally, the FQG administration notably ameliorated HUA-induced renal injury in rats. Through the pharmacodynamics of the HUA rat models and network pharmacology, it was found that XOD was a key pathway enzyme in UA metabolism. 18 XOD inhibitors were screened from FQG by UF-LC-MS, and 11 compounds with strong affinity were verified by SPR, molecular docking and CD spectroscopy. Conclusion In summary, flavonoids, organic acids and saponins may be the active components in FQG that alleviate HUA. The primary mechanism of FQG involves inhibiting XOD enzyme activity in the plasma to reduce UA production, alleviating renal tubular epithelial cell necrosis, tubulointerstitial injury, fibrosis, and urate deposition, ultimately exerting a therapeutic effect on HUA.
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American Journal of Translational Research
·
2021
·
IF 4.06
Shaochang Wu,Guidong Zhu,Dajin Zhou,Hongtao Xu,Longyou Zhao
Objective: This study was designed to demonstrate the accuracy of ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in detecting serum concentration of anti-schizophrenic drugs in patients with mental illness. Methods: The study participants were 186 schizophrenia patients treated in our hospital. Serum concentrations of anti-schizophrenic drugs in Chinese patients with mental illness were evaluated according to the reference intervals of drug therapy recommended in the guidelines. Results: Five drugs, namely Aripiprazole (ARI), Amisulpride (AMI), Olanzapine (OLA), Paliperidone (PAL) and Ziprasidone (ZIP) all showed good linearity within the linear range. The within-day precision of the above five drugs was all between 1.3%-8.9% and the inter-day precision was between 1.8%-7.6%, with within-day and inter-day relative standard deviations (RSDs) less than 15.00% and accuracy ranging from 87.00% to 106.73%. However, AMI had a mean blood concentration of 436.31±241.05 ng/mL (median concentration: 379.34 ng/mL), which was significantly higher than the reference range (100-320 ng/mL) recommended in the guidelines. Good recovery rates (86.21%-99.77%) were obtained after the samples were stored at room temperature for 24 h, at 4°C for 48h and at -20°C for half a year. Conclusions: Given that UPLC-MS/MS renders more accurate results in detecting the concentration of psychotropic drugs, it can be applied clinically to detect the concentration of therapeutic drugs in patients with mental illness.
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Coatings
·
2025
·Vol. 15
·期 3
·267
·
IF 2.8
Junyu Liu,Hansheng Li,Wencan Cui,Shihua Sang,Liang Guan,Chen Zhao
This study focuses on the preparation of a highly active surface-enhanced Raman spectroscopy (SERS) substrate, where Ag nanoparticles synthesized by the Lee–Meisel method are loaded onto the surface of GO@PVP nanoparticles through classical electrostatic attraction. The substrate was characterized by SEM and XPS analysis. The SERS-active substrate was utilized for the detection of phenanthrene in aqueous solutions, achieving a detection limit of 10−8g/L (5.6 × 10−11mol/L). In comparison to conventional Raman spectroscopy, this method exhibits a significant reduction in the detection limit and holds promise for trace polycyclic aromatic hydrocarbons (PAHs) monitoring in environmental samples.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2014
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IF 2.829
Yun Lei,Guanhong Xu,Fangdi Wei,Jing Yang,Qin Hu
A molecularly imprinted polymer coated stir bar (MIP-SB) using dopamine as a template was fabricated. The adsorptive capacity of the MIP-SB was almost 4 times over that of non-imprinted stir bar. The MIP-SB showed good extracting selectivity for dopamine when used to adsorb dopamine and its analogs. An analytical method to determine dopamine in urine sample was established by combining MIP-SB sorptive extraction with HPLC-fluorescence detector. The linear range of dopamine concentration was 0.378–18.9 ng/ml with correlation coefficient of 0.9990, and the limit of quantification was about 0.0945 ng/ml (S/N = 10). The recoveries of dopamine with spiked urine samples at three different levels were between 92.3 and 106.9%, and the relative standard deviations were within 13.2% ( n = 3). The method is simple and suitable for the determination of dopamine in human urine for clinical application.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xinru Fan,Wenhao Geng,Xinyue Zhang,Meng Li,Kexin Chang,Yongsheng Ma,Soottawat Benjakul,Qiancheng Zhao
The objective of this investigation was to isolate amur catfish myofibrillar protein (AMP), emulsify it with rice bran oil, and assess its application in the silver carp surimi gel. The proportion and addition of AMP/rice bran oil emulsion on the gel properties were compared. The emulsion stabilized by 3 % AMP was stable when an oil/water ratio of 0.70 and 0.75 was employed, as evidenced by its appearance, microstructure, and rheological characteristics. The L* , a* and whiteness values of gels observably elevated when the emulsion blended ( p < 0.05). The mechanical capacities (texture and rheology indicators) of surimi gels were dramatically improved with 5 % emulsion added ( p < 0.05). However, the addition of excessive emulsion (up to 15 %) impeded the formation of surimi protein network and lowered water retention ability of surimi gels. Moreover, 5 % emulsion was distributed uniformly throughout the gel matrix, giving rise to the development of a dense and uniform network with high water binding capacity. Furthermore, the study on protein solubility expressed that the addition of the emulsion facilitated protein-protein interactions in surimi gels. In summary, the addition of 5 % AMP stabilized rice bran oil emulsion could yield a stronger surimi gel with increased whiteness. The resulting gels could serve as healthy fat-rich surimi-based products.
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TALANTA
·
2019
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IF 4.916
Guifen Zhu,Guohao Cheng,Peiyun Wang,Wanwan Li,Yicong Wang,Jing Fan
A novel water compatible ciprofloxacin imprinted polymer is synthesized in water via a green, non-toxic and environmentally friendly polymerization process. Hydrophilic groups, including anionic chlorine, hydroxyl , and carbonyl oxygen provided by a bifunctional monomer comprising 1-allyl-3-vinylimidazole chloride and 2-hydroxyethyl methacrylate, are introduced into the imprinted material, which allows the polymer to interact strongly with imprinting molecule via hydrogen bonds , electrostatic and π-π dipole interactions in aqueous solution. Rebinding experiments show that the obtained molecularly imprinted polymer (MIP) presents special molecular recognition towards quinolone antibiotics (ciprofloxacin, levofloxacin and pefloxacin mesylate) in aqueous matrices. The adsorption process of ciprofloxacin on MIP and non-imprinted polymer (NIP) substrates involves spontaneous exothermic reactions , and the maximum rebinding capacities of ciprofloxacin on MIP and NIP at 25 °C are 19.96 and 8.86 mg g −1 , respectively. The excellent selectivity and hydrophilicity of this imprinted polymer makes it suitable for use as an adsorbent in solid phase extraction . Under the optimized conditions, the presented MIP-SPE protocol exhibits a wide linear range between 0.29 and 1.47 × 10 5 μg L −1 and has been successfully applied for the separation and enrichment of trace ciprofloxacin in real water, soil and pork samples with satisfactory recoveries of 87.33–102.50%. The proposed study implies the promising prospect of this green and water compatible MIP in highly effective recognition and separation of trace quinolones in complex matrics.
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BMC GENOMICS
·
2025
·Vol. 26
·期 1
·1-16
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IF 3.7
Liu Zhen,Ma Xiaoping,Zeng Xiangwen,Li Zhiguo,Liu Ruiguo,Luo Rongyan,Wang Weichen,Tahir Muhammad Salman,Wang Chengdong,Gu Yu
Alcohol dehydrogenase has been studied in regulation of fungal growth and development, stress response and pathogenesis, but its function in T. asahii remains unexplored. In this study, we analyzed the ADH gene family in T. asahii for the first time, identifying six ADH genes and containing conserved ADH_N and ADH_Zinc_N domains. We constructed an overexpression strain of the most significantly differentially expressed gene TaADH_like and compared its phenotypes with those of the wild-type strain, focusing on colony morphology, biofilm biomass, stress response, drug resistance, and pathogenicity. The results showed that TaADH_like overexpression reduced sensitivity to hypoxic conditions, altered the hyphae-to-yeast transition, and led to slower growth, decreased colonization ability, reduced tissue damage, and lower lethality. Increased osmotic stress sensitivity and the involvement of the HOG MAPK pathway in the hyphae-to-yeast conversion contributed to the reduced colonization capacity of T. asahii . Furthermore, the overexpression of TaADH_like promoted biofilm formation and led to a slight enhancement in fluconazole resistance in T. asahii . This study is the first to elucidate the function of the alcohol dehydrogenase gene in T. asahii , providing a foundation for future genetic research on this pathogen.
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Advanced Science
·
2025
·
IF 14.1
Wenwei Li,Yang Liu,Ming Wei,Zhichao Yang,Zhaoyu Li,Zezhong Guo,Liang Yan,Yang Lu,Hao Tang,Bofeng Li,Wei Huang
Osteoarthritis (OA) is a low-grade inflammatory disease that is highly associated with severe hyperplasia of the synovial membrane and the degeneration of cartilage. Interleukin-10 (IL-10), has been extensively studied, while its receptor, IL-10Rα, has not been widely mentioned in the context of OA. A significant difference is found in the expression of IL-10Rα in synovial macrophages from normal and OA patients, along with a marked increase in the glycolytic activity of synovial macrophages. In IL-10Rα Lysm OA mice, the specific deficiency of IL-10Rα exacerbated the progression of OA. Mechanistically, hypoxia-inducible factor-1α (HIF-1α) is identified as a key transcription factor, and its inhibition significantly weakened the glycolytic process. Additionally, differences in ferroptosis of chondrocytes are observed. After co-culturing the two types of cells in vitro, a significant connection is found between the glycolytic state of synovial macrophages and the ferroptosis of chondrocytes. To achieve targeted therapy, MI@UN, a biomimetic nanoparticle encapsulating NO-prednisolone in UIO-66-NH 2 , surface-modified with IL-10, and coated with macrophage membranes (MM), is developed. It significantly slows osteoarthritis progression in mice. This offers new insights into OA pathogenesis, highlighting IL-10Rα as a therapeutic target and supporting MI@UN's translational use for OA treatment.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Yaning Liu,Shengwei Yin,Liuyi Hu,Ruojian Ma,Min Fan,Meiqing Feng,Ruyi Fang,Zhen Xiao,Jun Zhang,Yongping Gan,Hui Huang,Xinping He,Xinhui Xia,Yanxian Jin,Xinyong Tao,Wenkui Zhang,Yang Xia
The interfacial failure of solid polymer electrolytes (SPEs) with Li anode, particularly those containing succinonitrile (SN) types, has significantly hindered the practical development of solid-state lithium-metal batteries. Herein, we introduce silicified montmorillonite (SiO 2 -MMT) into polyethylene oxide (PEO)/SN-based SPEs to facilitate the in-situ formation of a LiF-rich phase, thereby significantly enhancing the electrolyte/Li anode interface stability. Specifically, the SiO 2 -MMT strongly anchors the SN molecules, preventing their migration to the Li anode side. Meanwhile, the TFSI − reacts competitively with Li to generate a dense LiF phase, further blocking the SN chemical corrosion of Li metal. Furthermore, the SiO 2 -MMT greatly accelerates the Li + transportability and enhances the thermal stability and mechanical strength of the composite electrolyte. Consequently, the composite electrolyte has a high ionic conductivity of 1.2 × 10 −4 S cm −1 at 30 °C, which almost twice as higher as the counterpart. As for the solid-state battery, the LiFePO 4 ||Li cell demonstrates remarkable cycling stability, achieving a high discharge capacity of 141.7 mA h g −1 with a remarkable capacity retention of 90.2 % after 350 cycles at 0.5 C. Additionally, the high electrochemical window (4.6 V) ensures compatibility with high-voltage cathode materials. The NCM83||Li cell demonstrates a satisfactory initial discharge capacity and excellent capacity retention of 179.2 mA h g −1 and 81.8 % at 0.2 C after 100 cycles. This work offers a new insight for achieving a highly stable electrolyte/Li anode interface in SN-containing SPEs, facilitating the practical application of solid-state lithium metal batteries with enhanced safety and lifespan.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Huadong Fu,Rui Chen,Yong Xie,Zheng Wei,Yan Qin,Zhixiong Huang
Element-hybrid phenolic aerogel composites are often utilized as lightweight thermal protection materials for aerospace craft. Zr-phenolic (Zr-PR) aerogels have been extensively studied due to their exceptional high-temperature resistance. However, the phase transformation of ZrO2, the pyrolysis product of Zr-PR, caused the volume changes and internal stresses, greatly limiting the application of Zr-PR. In this work, a small amount of nano-Y2O3 particles was directly doped into the Zr-PR aerogel composites to form the nanoscale mosaic structure. This aerogel composite exhibited properties of low density (0.51 g/cm3) and low thermal conductivity (<0.151 W/(m K)); moreover, it showed a significant improvement in oxidative ablation resistance with a 33% reduction in the linear ablation rate. The research indicated that the doping of nano-Y2O3 effectively addressed the adverse effects associated with ZrO2 phase transformation by in situ rapidly forming (<60s) the dense yttria-stabilized zirconia (YSZ) thermal barrier layer on the ablation surface, which significantly delayed the thermal-oxidative ablation of the composites. Additionally, the pyrolytic carbon (PyC) on the ablation surface can form abundant multilayered graphene structures due to the catalytic graphitization effect produced by the solid-solution reaction of YSZ, which further enhanced the oxidation resistance. This work provides a simple and efficient approach to improve the ablation performance of Zr-PR aerogel composites, broadening their application in extreme thermal environments.
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