Biotechnology for Biofuels and Bioproducts
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2023
·Vol. 16
·期 1
·1-13
·
IF 0
Zhang Yang,Meng Wenchang,He Yuting,Chen Yuhui,Shao Mingyu,Yuan Jifeng
Background Whole-cell biocatalysis has been exploited to convert a variety of substrates into high-value bulk or chiral fine chemicals. However, the traditional whole-cell biocatalysis typically utilizes the heterotrophic microbes as the biocatalyst, which requires carbohydrates to power the cofactor (ATP, NAD (P)H) regeneration. Results In this study, we sought to harness purple non-sulfur photosynthetic bacterium (PNSB) as the biocatalyst to achieve light-driven cofactor regeneration for cascade biocatalysis. We substantially improved the performance of Rhodopseudomonas palustris -based biocatalysis using a highly active and conditional expression system, blocking the side-reactions, controlling the feeding strategy, and attenuating the light shading effect. Under light-anaerobic conditions, we found that 50 mM ferulic acid could be completely converted to vanillyl alcohol using the recombinant strain with 100% efficiency, and > 99.9% conversion of 50 mM p -coumaric acid to p -hydroxybenzyl alcohol was similarly achieved. Moreover, we examined the isoprenol utilization pathway for pinene synthesis and 92% conversion of 30 mM isoprenol to pinene was obtained. Conclusions Taken together, these results suggested that R. palustris could be a promising host for light-powered biotransformation, which offers an efficient approach for synthesizing value-added chemicals in a green and sustainable manner. Graphical Abstract
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Frontiers of Optoelectronics
·
2023
·Vol. 16
·期 1
·1-11
·
IF 5.4
He Jungang,Ge You,Wang Ya,Yuan Mohan,Xia Hang,Zhang Xingchen,Chen Xiao,Wang Xia,Zhou Xianchang,Li Kanghua,Chen Chao,Tang Jiang
Lead selenide (PbSe) colloidal quantum dots (CQDs) are suitable for the development of the next-generation of photovoltaics (PVs) because of efficient multiple-exciton generation and strong charge coupling ability. To date, the reported high-efficient PbSe CQD PVs use spin-coated zinc oxide (ZnO) as the electron transport layer (ETL). However, it is found that the surface defects of ZnO present a difficulty in completion of passivation, and this impedes the continuous progress of devices. To address this disadvantage, fluoride (F) anions are employed for the surface passivation of ZnO through a chemical bath deposition method (CBD). The F-passivated ZnO ETL possesses decreased densities of oxygen vacancy and a favorable band alignment. Benefiting from these improvements, PbSe CQD PVs report an efficiency of 10.04%, comparatively 9.4% higher than that of devices using sol-gel (SG) ZnO as ETL. We are optimistic that this interface passivation strategy has great potential in the development of solution-processed CQD optoelectronic devices. Graphical Abstract
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 44
·17045-17065
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IF 4.5
Fan Qingwen,Song Chaoyun,Zhang Yuchun,Ren Gangxin,Sun Yongmei,Fu Peng
The development of new materials for electromagnetic wave absorption and energy storage has gained significant attention due to their widespread applications. This study introduces a novel approach to address the stacking issue of MXenes and enhance the electromagnetic shielding capabilities of porous carbon by combining them with jujube shell porous carbon. This composite material exhibits outstanding electromagnetic shielding properties and electrochemical energy storage performance. The addition of polar acetone enhances the interfacial interaction between the carbon matrix and Ti 3 C 2 Tx, resulting in a remarkable return loss of − 36.1 dB for AJSPC&Ti-A, signifying the absorption of 99.9% of incident electromagnetic waves. The three-dimensional (3D) porosity and two-dimensional (2D) laminar structure of the composite prolong the propagation of electromagnetic waves, leading to multiple reflection and scattering effects. Furthermore, the 3D porous structure provides abundant channels and active sites for the storage and transport of electrolyte ions. In electrochemical testing, AJSPC&Ti-A achieves a specific capacity of 156.1 F/g at 1 M Na 2 SO 4 and 0.5 A/g, surpassing the performance of alkaline electrolytes. In a symmetrical capacitor configuration, this composite demonstrates an energy density of 8.4 Wh/kg, accompanied by a power density of 193.0 W/kg. Density functional theory analysis reveals changes in the interface between porous carbon and Ti 3 C 2 Tx, attributed to electronegativity differences and increased spin density. These alterations manifest in the redistribution of charge density and uneven electron distribution. Additionally, the s and d orbitals contribute to the enhancement of density of states, promoting multiple polarization relaxation and charge transfer in AJSPC&Ti-A. This research introduces a new class of composites that exhibit dual functionality, offering efficient electromagnetic shielding and excellent electrochemical energy storage capabilities. Graphical Abstract
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Nano-Micro Letters
·
2023
·Vol. 16
·期 1
·1-17
·
IF 26.6
Wang Kangwang,Hu Zhuofeng,Yu Peifeng,Balu Alina M.,Li Kuan,Li Longfu,Zeng Lingyong,Zhang Chao,Luque Rafael,Yan Kai,Luo Huixia
The S-vacancies result in the change of d-band electronic state of Mo.
An internal quantum efficiency of 94.01% at 380 nm for photocatalytic CO 2 reduction reaction (CO 2 RR).
The Mo–S bridging bonds optimize adsorption energies and accelerate CO 2 RR kinetics.
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Nano-Micro Letters
·
2023
·Vol. 16
·期 1
·1-20
·
IF 26.6
Qin Xianhui,Chen Zhongrong,Shen Lingxiao,Liu Huilan,Ouyang Xilin,Zhao Gang
Cryopreservation of red blood cells (RBCs) provides great potential benefits for providing transfusion timely in emergencies. High concentrations of glycerol (20% or 40%) are used for RBC cryopreservation in current clinical practice, which results in cytotoxicity and osmotic injuries that must be carefully controlled. However, existing studies on the low-glycerol cryopreservation of RBCs still suffer from the bottleneck of low hematocrit levels, which require relatively large storage space and an extra concentration process before transfusion, making it inconvenient (time-consuming, and also may cause injury and sample lose) for clinical applications. To this end, we develop a novel method for the glycerol-free cryopreservation of human RBCs with a high final hematocrit by using trehalose as the sole cryoprotectant to dehydrate RBCs and using core–shell alginate hydrogel microfibers to enhance heat transfer during cryopreservation. Different from previous studies, we achieve the cryopreservation of human RBCs at high hematocrit (> 40%) with high recovery (up to 95%). Additionally, the washed RBCs post-cryopreserved are proved to maintain their morphology, mechanics, and functional properties. This may provide a nontoxic, high-efficiency, and glycerol-free approach for RBC cryopreservation, along with potential clinical transfusion benefits.
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Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy
·
2023
·
IF 1.6
Huang Mingjun,Wu Fanchang,Zuo Xue,Liu Jinman,Yu Wenqian,Xie Ruiye,Liu Guoqing,Tan Qiwen,Wang Qi,Liang Yong
Plant-based medicines derived from traditional Chinese medicine have garnered widespread attention for their potential in the prevention and treatment of various diseases, offering superior oral bioavailability compared to the use of single drug formulations. Nonetheless, the precise biological mechanisms and interactions between compounds and enzymes remain inadequately understood. This study delves into the inhibitory interactions of quercetin with cytochrome P450 and P-glycoprotein, leading to a synergistic enhancement of osthole bioavailability in rat plasma. The results indicate that quercetin demonstrates concentration-dependent and uncompetitive inhibition of liver microsomal cytochrome P450 proteins 3A, with an observed IC 50 value of 34.17 μM. Additionally, quercetin notably suppresses the expression of P-glycoprotein in the small intestines of rats, resulting in a significant 52.9% reduction ( p < 0.001) and a corresponding downregulation of its associated mRNA Mdr1b level. The administration of quercetin alongside osthole in rats leads to a marked increase of over 80% ( p < 0.001) in osthole blood concentration, as determined by pharmacokinetic analysis, surpassing the effects of pure osthole usage. This multifunctional attribute of quercetin, along with the observed synergistic changes in pharmacokinetics, holds promise for shedding light on the interplay of drug–drug/enzyme interactions in the context of medicinal plants. Graphical abstract
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Journal of Food Measurement and Characterization
·
2023
·
IF 3.4
Wei Qingyi,Dong Qirong,Pu Hongbin,Sun Da-Wen
Aquaculture fungicide causes threats to the human community and their sensitive detection are thus essential for food safety. Herein, MoS 2 /g-C 3 N 4 @Au heterojunction-based recyclable SERS substrate was developed for fungicide detection. The optimal substrate exhibited good SERS performance with a detection limit as low as 9.908 × 10 −5 mg L −1 for malachite green (MG). Furthermore, an MCN@Au-based photodegradation of crystal violet (CV) under simulated light irradiation was realized, resulting in a degradation rate of 95.6%. With the dual function of the as-synthesized materials, good reusability of the substrates for at least six “detection-degradation” cycles was achieved. In addition, the MG in prawn extract could be sensitively determined with a satisfactory recovery from 91.60 to 124.00%. This work paves an avenue for the fabrication of heterojunction-based recyclable SERS substrates and provides a novel weapon for food safety protection.
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Science China-Materials
·
2023
·Vol. 66
·期 12
·4853-4864
·
IF 8.1
Chen Ying,Kuang Peipei,Wang Yushu,Luo Sihan,Shi Jingyi,Lv Xiaowei,Liu Yupeng,Fan Quli
Conductive hydrogels have emerged as versatile materials with promising applications in various fields, as they possess the ability to convert external forces and deformations into electrical signals, enabling them to sense physical and chemical stimuli. However, the development of conductive hydrogels faces a challenge in balancing conductivity and mechanical strength demands. We incorporated lignin-tannic acid (lignin-TA) particles as physical crosslinkers to enhance their mechanical properties. Additionally, the multi-hydroxyl structure of hydroxypropyl cellulose molecules facilitated the formation of a semi-interpenetrating polymer network through hydrogen bond interactions. Furthermore, the bond strength between the lignin-TA-polyacrylamide networks was intensified, resulting in an elevation of their conductivity. This enhancement was achieved by harnessing the electrostatic forces and metal coordination interactions between Fe 3+ ions and TA molecules. The resulting hydrogel exhibited outstanding mechanical characteristics due to its unique network structure, combining physical and chemical bonds. Taking advantage of the hydrogel’s unique network structure, we successfully utilized its self-adhesion, self-healing capabilities, electromechanical properties, and biocompatibility to monitor human activities and cell proliferation.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-13
·
IF 16.6
Chen Na,Du Na,Shen Ruichen,He Tianpei,Xi Jing,Tan Jie,Bian Guangkai,Yang Yanbing,Liu Tiangang,Tan Weihong,Yu Lilei,Yuan Quan
Microbial communication can drive coordinated functions through sensing, analyzing and processing signal information, playing critical roles in biomanufacturing and life evolution. However, it is still a great challenge to develop effective methods to construct a microbial communication system with coordinated behaviors. Here, we report an electron transfer triggered redox communication network consisting of three building blocks including signal router, optical verifier and bio-actuator for microbial metabolism regulation and coordination. In the redox communication network, the Fe 3+ /Fe 2+ redox signal can be dynamically and reversibly transduced, channeling electrons directly and specifically into bio-actuator cells through iron oxidation pathway. The redox communication network drives gene expression of electron transfer proteins and simultaneously facilitates the critical reducing power regeneration in the bio-actuator, thus enabling regulation of microbial metabolism. In this way, the redox communication system efficiently promotes the biomanufacturing yield and CO 2 fixation rate of bio-actuator. Furthermore, the results demonstrate that this redox communication strategy is applicable both in co-culture and microbial consortia. The proposed electron transfer triggered redox communication strategy in this work could provide an approach for reducing power regeneration and metabolic optimization and could offer insights into improving biomanufacturing efficiency.
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Advanced Fiber Materials
·
2023
·Vol. 5
·期 6
·2080-2098
·
IF 16.1
Yan Biaobiao,Bao Xueming,Gao Yilei,Zhou Man,Yu Yuanyuan,Xu Bo,Cui Li,Wang Qiang,Wang Ping
Two-dimensional transition metal carbide/nitride (MXene)-based textiles have been developed in many fields; however, the high sensitivity to oxidation and weak interfacial bonding hinder their applications. Herein, we present a strategy for the preparation of a highly antioxidative MXene@gallic acid (MXene@GA, MG) hybrid dispersion, and further covalently grafted it onto carboxylated cotton fabric through interaction with metal ions (Fe 3+ ) for fabricating wearable multifunctional textiles. Due to the cross-linking effect of Fe 3+ and the remarkable antioxidant activity of natural polyphenol GA, the MG coatings firmly adhere to the textile surfaces and can withstand conventional washing, exhibiting favorable service stability and potential application prospects. Moreover, the obtained MG-decorated textile has the inherent characteristics of good breathability, moisture permeability, flexibility, and biocompatibility of the original fabric, which are conducive to the wearability of smart devices. Furthermore, by utilizing the outstanding conductivity (~ 330 S/m) and photothermal convertibility of the MG coating, the functional textile achieves high electromagnetic interference (EMI) shielding efficiency (~ 35 dB), excellent dual-driven ( Joule and solar) heating warmth retention, and infrared thermal camouflage. Due to the green and scalable preparation process, favorable durability, excellent comfort, and multifunctionality, the MG-decorated textiles are anticipated to be promising candidates for the next generation of smart wearable personal protective clothing. Graphical Abstract
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RARE METALS
·
2023
·Vol. 42
·期 11
·3829-3838
·
IF 8.8
Liu Xue-Feng,He Jiang-Feng,Li Ya-Ge,Li Hang,Lei Wen,Jia Quan-Li,Zhang Shao-Wei,Zhang Hai-Jun
Porous SiC ceramics (PSCs) are promising lightweight and efficient thermal insulators that can evade infrared detection by reducing the surface temperature of the protected object, which plays a crucial role in the development of new military equipment. However, the controllable synthesis of PSCs with both hierarchical pore structure and thermal/mechanical stability remains challenging. In this work, such PSCs were prepared by a facile foam-gelcasting/solid-state reaction method, using silicon powders and glucose-derived carbon as starting materials. The favorable dispersibility and wettability of hydrophilic carbon microspheres and the in-situ formed SiC guarantee the highly porous structure (92.8% porosity), comparable bulk density (0.20 g·cm −3 ) and reasonable mechanical property of the product. The designed PSCs performed outstanding high-temperature performance, especially thermal insulation in both oxidizing and inert atmospheres. More importantly, the composite architecture of PSCs and low emissivity layer (Al foil) exhibited desirable infrared stealth property (at a temperature up to 1100 °C), significantly extending the operating temperature range of thermal camouflage material. The unique combination of excellent properties would make PSCs a potential candidate material for future thermal protection and infrared stealth applications in an extreme environment. Graphical abstract
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Bio-Design and Manufacturing
·
2023
·
IF 7.9
Zhao Pengcheng,Wang Biling,Wang Lu,Fu Zexin,Hu Jun,Liu Yande,Wang Ji,He Yong
Prosthesis implantation and fat transplantation are common breast reconstruction methods. In general, prosthesis implantation alone does not achieve a realistic enough appearance, and fat transplantation alone is difficult to achieve in the correct capacity. To date, no reports have focused on methods of combining fat with implanted prostheses for breast reconstruction. Using a newly designed bionic ink (i.e., polyether F127 diacrylate (F127DA) & poly(ethylene glycol) diacrylate (PEGDA)) and projection-based three-dimensional bioprinting (PBP), we report the development of a new method for printing porous prostheses. PEGDA was used to improve the printing precision of the prosthesis by increasing the gel point of F127DA and reducing the impact of external temperature. The compression modulus of the printed prosthesis was very close to that of prostheses currently used in clinical practice and to that of natural breasts. Finally, stromal vascular fraction gel (SVF-gel), a human fat extract, was injected into the pores of the synthesized prostheses to prepare a prosthesis mixed with adipose tissue. These were implanted subcutaneously in nude mice to observe their biological performance. After 14 and 28 days of observation, the prosthesis showed good biocompatibility, and adipose tissues grew well in and around the prosthesis. This result shows that a porous prosthesis containing pre-placed adipose tissues is a promising breast reconstruction material. Graphic abstract
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