ANALYTICAL CHEMISTRY
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2020
·
IF 6.785
Lu Shi,Feifei Cao,Limin Zhang,Yang Tian
The development of switches responding to specific pH changes was particularly useful in wide application fields. Owing to flexible switches simulated by pH, i-motif DNAs are widely used as a pH sensor. But its character of structure transition strongly dependent on acidic pH severely hampers the application of i-motif DNA in physiological media. Herein, we report the stable i-motif structure formed at a physiological pH triggered by spatial confinement of silica nanochannels. Three classic DNA chains containing 21-mer i-motif domain base-pairs and a single-stranded multiply (T)n spacer, 5′-COOH-(T)n-CCCTAACCCTAACCCTAACCC-3′, were employed to evaluate the enhanced stability of i-motif structure. Compared to their free states in a dilute solution, the transition pH of all i-motif DNAs decorated in nanochannels remarkably shifts toward a neutral pH. Moreover, the transition midpoint can be tuned sensitively over the physiologically relevant pH range through slightly varying the length of T base spacer. Density functional theory (DFT) calculations validate that the increased proton density in a nanochannel triggers the formation of an i-motif structure under a neutral pH. Finally, this i-motif DNA based nanochannels electrode was successfully employed to monitor pH in brain microdialysates followed by cerebral ischemia. The present approach is not limited by fundamental investigation for DNA conformation but may extend toward the manipulation of i-motif based structures for artificial molecular machines and signaling systems.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2020
·
IF 3.209
Haifeng Li,Shuang Wang,Fan Cui,Beibei Zhuo,Shuang Wang,Chenrui Zhao,Weilu Liu
Puerarin, an important isoflavone, has been widely used for the treatment of angina and hypertension. In this work, we developed a novel electrochemical sensor for the detection of puerarin based on the hybrid of reduced graphene oxide (RGO) and molecularly imprinted polymer (MIP). The RGO/MIP sensor functions by target puerarin recognition and electro-oxidization via a two-proton and two-electron process, enabling the detection of puerarin with good selectivity and high sensitivity. The MIP layer was integrated on the surface of RGO by the electro-co-polymerization of o-phenylenediamine (monomer) and puerarin (template), resulting in high surface area, binding capacity, good conductivity and faster mass transfer. The nanostructure of the RGO/MIP hybrid was demonstrated using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Experimental conditions involved in the sensor fabrication process were evaluated. Under the optimized condition, a wide linear range (0.02 μM ∼ 40 μM) and a low detection limit (0.006 μM) were achieved. The sensor was applied to detect puerarin in human urine and injection samples, and the result was comparable with that of the gold standard method of high-performance liquid chromatography (HPLC), indicating a promise in the further application to pharmacokinetics or therapeutic drug monitoring.
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SENSORS AND ACTUATORS B-CHEMICAL
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2020
·
IF 7.1
Mingyue Luo,Xin Xue,Honghong Rao,Hongqiang Wang,Xiuhui Liu,Xibin Zhou,Zhonghua Xue,Xiaoquan Lu
Traditional colorimetric sensors often suffer from the low color resolution and the not well-defined boundary between the yes and no answers, especially for distinguishing the similar color readouts with the naked eye. Herein, a simple strategy is communicated for greenly converting colorimetric sensor into a photothermal sensor, which is based on the simple color-to-thermal (temperature) signal readout conversion. The colorimetric system based on simply mixing commercially available chemicals of 3,3,5,5-tetramethylbenzidine (TMB) and silver ions (Ag + ), provides a straightforward blue color readout owing to the generation of oxidized TMB (TMBox). In the presence of the target alkaline phosphatase (ALP), ascorbic acid phosphate (AAP) in the system could be hydrolyzed into ascorbic acid (AA), subsequently reducing the blue colored TMBox into colorless TMB. The blue-to-colorless change-based colorimetric system also exhibits the highly sensitive near-infrared (NIR) laser-driven photothermal response, allowing the successful thermometer-detectable signal-based assay for point-of-care testing (POCT) of ALP levels just using a common thermometer. This study not only demonstrated a regulatory strategy to convert color-based signal readout into thermal (temperature)-based signal readout, but also established a facile method to design affordable thermometric sensors for POCT and instrument-free bioanalysis , especially in low-resource settings.
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JOURNAL OF HAZARDOUS MATERIALS
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2020
·
IF 9.038
Yaoping Hu,Zhijin Gao
Crystal violet (CV), a hazardous dye, poses a serious threat to the environment and human health. This motivates us to develop a facile method for its sensitive detection. Herein, we demonstrate a rapid sensing of CV using a novel fluorescent nanomaterial, yellow emissive Se,N-codoped carbon dots (CDs). CDs with an intense photoluminescence peak at 566 nm are synthesized by a hydrothermal technique using selenourea and o-phenylenediamine as precursors. This material shows a high quantum yield of up to 16.7 %. It is found that the yellow fluorescence of CDs can be selectively quenched by CV, which makes them promising for CV sensing. The linearity is obeyed in the range of 0.02–1.60 μM, and the limit of detection is as low as 7.3 nM. After detailed investigations, the inner filter effect is proposed to be the sensing mechanism. For practical usage, the newly built method is applied to determine the trace amount of CV in fish tissue samples, and satisfactory results are obtained.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2020
·Vol. 24
·期 3
·583-590
·
IF 2.646
Yu Zhipeng,Kong Chuncai,Lv Jian,Ma Bo,Zhang Xiaojing,Yang Zhimao
Designing highly active material to fabricate high-performance glucose sensor was great importance for diabetes treatment. In this work, we develop a novel nanocomposite composed of multilevel nanostructure with ultrathin Cu x O nanoflakes anchored on Cu 2 O nanowires, directly on Cu foil and its application as non-enzymatic glucose sensor. Due to more active sites endowed by the ultrathin Cu x O nanoflakes, the as-prepared Cu x O nanoflakes/Cu 2 O nanowires@Cu nanoarray exhibit high sensitivity (3.59 mA mM −1 cm −2 ), low detection limit (0.69 μM), high selectivity, fast amperometric response (~ 2 s) and excellent reliability in human serum. And the sensitivity of ultrathin Cu x O nanoflakes/Cu 2 O nanowires@Cu nanoarray was 1.9-fold than that of Cu 2 O nanowires@Cu nanoarray. Such novel multilevel nanoarray synthesized by convenient method provides a facile way to design metal oxide nanomaterial for promising non-enzymatic glucose sensor.
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ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Qiwen Wang,Haipei Si,Lihui Zhang,Ling Li,Xiaohong Wang,Shengtian Wang
A novel electrochemical sensor based on ZrO 2 and ZnO hybrid nanocomposites was developed for simultaneous determination of epinephrine (EA), uric acid (UA) and folic acid (FA) with the highly selective and ultrasensitive. The nanocomposites have been synthesized by chemical precipitation and thermal calcine method with economical, eco-friendly and practical nature. Their structure and electrochemical properties were investigated by X-ray diffraction (XRD), X-ray photo electron spectroscopy (XPS), scanning electron microscopy (SEM) and electrochemical techniques. The results reveal that the ZrO 2 /ZnO nanocomposites can possesses highly exposed catalytic sites, favorable conductivity, and the sensor excellent signal response for EP, UA and FA under the optimal condition. The electrochemical sensing platform has a low detection limit of 0.039 μM, 0.29 μM and 0.037 μM and a wide detection range of 0.8–420 μM, 10–2400 μM and 2–480 μM, respectively. It was also tested with a human blood serum sample at physiological pH with recovery 97.3–103.8% and relation standard deviation less than 5%. It indicates that the electrochemical sensors has a hopeful capacity of extensive applications in bioanalysis and diseases diagnosis.
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MICROCHIMICA ACTA
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2020
·Vol. 187
·期 2
·1-8
·
IF 6.232
Hou Chuantao,Zhang Xueli,Wang Lei,Zhang Fan,Huang Xiaolian,Wang Zonghua
A freestanding and flexible buckypaper modfied with CoP/Co (CoP/Co-BP) is described. It has a sponge-like nanostructure and is shown to enable improved nonenzymatic sensing of glucose. The CoP/Co-BP was prepared by first depositing a uniform layer of ZIF- 67 crystals on BP, followed by two steps of pyrolysis treatment and phosphidation under an argon atmosphere. The morphology and structure of the material were characterized by scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The electrochemical properties were investigated by cyclic voltammetry and amperometric response. The amperometric sensor, best operated at 0.45 V (vs. SCE) at pH 13 has a linear range that extends from 0.5 μM to 1.8 mM of glucose, a 0.2 μM detection limit (at S/ N = 3), and a sensitivity of 6427 μA mM −1 cm −2 in alkaline solution. This is mainly attributed to the synergistic effect between the highly active CoP nanostructure and BP which results in excellent conductivity. The uniformly distributed CoP nanoparticles in the network of BP prevent the formation of close-packed structure and facilitate electron transfer. The sensor has good selectivity and excellent long-term stability. It was applied to the determination of glucose in spiked human serum, and satisfactory results were obtained.
Schematic presentation of a freestanding and flexible buckypaper modfied with CoP/Co. It has a sponge-like nanostructure and exhibits improved catalytic activity toward glucose oxidation. This material was used for high-performance electrochemical glucose sensing.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2020
·
IF 3.807
Lili Xiao,Lin Jia,Shengzhi Zhao,Xiaoying Tang,Chengjie Zhu,Huan Huang,Jin Jiang,Mingtian Li
A kind of one-step, solvent-free, environment-friendly method is always highly appreciated in synthesis of electrocatalyst . In this work, MnO nanoparticles were coated by sheet-like carbon (MnO/C) for building a three-dimensional electrocatalyst with interconnected mesopores . Moreover, the unique porous structure of MnO/C can be facilely achieved through a one-step solvent-free calcination . In the calcination, 2-methylimidazole (2-MeIm) decomposed into carbon framework at the catalysis of MnO. The interconnected pores consisted of sheet-like carbon can significantly reduce mass transfer resistances and prevent the aggregation of MnO. Therefore MnO/C modified glassy carbon electrode (GCE) exhibited high activities in simultaneous detection of dopamine (DA) and uric acid (UA), with the sensitivities of 9.92 μA μM − 1 for DA, 8.42 μA μM − 1 for UA. The limits of detection were calculated as 0.009 μM and 0.03 μM for DA and UA, respectively. Moreover, MnO/C has promising application in detection of human urine and serum due to the satisfactory recoveries.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Xiaoying Guo,Xinyu Wang,Dongwei Huang,Jinjie Zhang,Dalun Xu,Wenge Yang,Qijie Hu,Zhongxiang Fang,Tao Huang
A new analytical method was developed to determine total purine content in fish meat. The analysis principle is that total purine extracted from fish meat are turned into uric acid by diazotization reaction and xanthine oxidase reaction. Then, the content of uric acid is measured by surface-enhanced Raman spectroscopy (SERS). The results showed that the optimal conditions for the diazotization reaction were at 60 °C for 40 min, using 0.16 M HCl, and 3.00 g/L NaNO 2 . The optimal xanthine oxidase reaction conditions were using 100 U/L xanthine oxidase, conducting at 30 °C and pH 7.0 for 40 min. The calibration curve established at the characteristic Raman peak of 631 cm −1 had a good linear relationship (R 2 = 0.9864). The recovery rate was between 101.7% and 105.0%. Therefore, the total purine content in the fish meat can be quantitatively determined with this new method.
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NANOTECHNOLOGY
·
2020
·Vol. 31
·期 32
·325502
·
IF 3.551
Nianfeng Shi,Shupei Sun,Bo Zhang,Qian Du,Yanxin Liao,Xiaoming Liao,Guangfu Yin,Zhongbing Huang,Ximing Pu,Xianchun Chen
Co(OH)2 nanosheets/Cu(OH)2 nanorods composite electrodes for non-enzymatic glucose detection were fabricated by electrodepositing Co(OH)2 nanosheets on Cu(OH)2 nanorods substrate grown directly on the copper sheet via a simple one-step reaction. The Co(OH)2 nanosheets/Cu(OH)2 nanorods composite electrode was characterized by scanning electron microscopy, energy dispersive x-ray spectroscopy, x-ray diffraction and x-ray photoelectron spectroscopy. The glucose sensing performance of the composite electrode was investigated by cyclic voltammetry and chronoamperometry. The composite electrode shows high sensitivity of 2366 µA mM−1 cm−2 up to 2 mM with a lower detection limit of 0.17 mM (S/N = 3). The composite electrode is highly selective to glucose in the presence of various substances that always co-exist with glucose in real blood samples. The response of the composite towards human blood serum was in good agreement with that of commercially available glucose sensors, suggesting that a promising electrode material for highly sensitive and selective non enzymatic detection of glucose can be envisioned.
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APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Di Zhu,Huiyuan Ma,Qingfang Zhen,Jianjiao Xin,Lichao Tan,Chunjing Zhang,Xinming Wang,Boxin Xiao
Epinephrine (EP) is a significant neurotransmitter, energizes and maintains the various body systems and plays a pivotal role in the response to stressful conditions of blood pressure and blood sugar level. Herein, a novel epinephrine sensor based on the hierarchical flower-like zinc oxide nanosheets (flower-like ZnO) embedded into three-dimensional ferrocene-functionalized graphene framework (3D graphene@Fc) was successfully prepared, using a facile route of one-pot in situ hydrothermal growth method. Benefiting from the unique structural advantages, meanwhile integrated with synergistic effect of components, the flower-like ZnO/3D graphene@Fc exhibits excellent sensing performance for epinephrine with a wide linear range of 0.02 to 216 μM, a low detection limit of 0.0093 μM, high anti-interference and good cyclic stability. Interestingly, it is unexpectedly found that the flower-like ZnO/3D graphene@Fc has sensing ability for EP’s oxidized derivative (QA) during the oxidation process of EP, along with a linear range of 0.192 to 527 μM and a detection limit of 0.1 μM for QA. More importantly, acceptable recovery obtained from the real sample test, confirming the feasibility of this biosensor for practical application.
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AICHE JOURNAL
·
2020
·Vol. 66
·期 8
·e16280
·
IF 3.519
Xiaobin Jiang,Mingguang Han,Zeqiu Xia,Jin Li,Xuehua Ruan,Xiaoming Yan,Wu Xiao,Gaohong He
Droplet evaporative crystallization on microscale heterogeneous surface is a vivid topic in chemical engineering, bioengineering, nanomaterials, and so on. Here, 3D printed interfacial matrix platform with regular pillar convexity and tunnel structure is fabricated to reveal the mechanism of the interfacial micro droplet crystallization. Element-based rotation volume model is established to simulate the concentration and nucleation barrier distribution during the microscale process. Sodium urate monohydrate and NaCl crystallization on the pillar convex structure both confirm that confined capillary flow in the micro droplet and initial nucleation condition dominate the nucleation, growth control and particle distribution. Droplet crystallization stretches over the tunnel structure reveal an interesting phenomenon that two regions possessing distinct-different nucleation barriers can isolated obtain the crystal particles from nanoscale to even millimeter scale. The fabricated platform and the capillary circulation transfer theory unfold a potential approach to harvest high value-added crystals with specific morphology and desire sizes distribution.
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危险品化学品经营许可证（带存储）