CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Yong Chen,Xiaoxiao Zhu,Xinping Wang,Yunpeng Su
A facial and cost-effective protocol preparing multi-hollow silicalite-1 (S-1) zeolite was proposed. That is, treating the parent zeolite with tetrapropylammonium hydroxide (TPAOH) solution at low temperature for a period prior to recrystallization. In this protocol, the geometric structure of the product could be highly controlled from hollow to sponge-like multi-hollow configuration, by modulating the period. It was demonstrated that the key role of this step performed before the recrystallization is to create some deep gullies on the parent zeolite that are necessary for constructing the desired multi-hollow structure. This strategy was also succeeded for fast confining single- or bi-metal nanoparticles in much high dispersion within the multi-hollow S-1 without any protective agent addition. It was proved that this protocol is very effective for dispersing Pt within the sponge-like S-1, even for preparing the Pt@S-1 composite with the Pt content as high as 1.67 wt.%. As a specialty of the protocol, it is that, the protocol always gives desired open mesopores to the product material. The Pt@S-1 and PtSn@S-1 exhibited much higher activity for CO oxidation and propane dehydrogenation than the corresponding conventional catalysts.
研究领域:
材料化学
·
纳米催化
·
多孔材料
·
化学工程
·
工业催化
✓ 已验证
DOI
高影响因子
Science of Advanced Materials
·
2014
·Vol. 6
·期 9
·1936-1942
·
IF 2.908
Li Xiang,Ding Xuelian,Wang Gongke,Zhou Yixuan,Hou Ruipeng,Gao Shuyan,Lu Yan
In this work, we have developed a simple and highly sensitive fluorescent biosensor for the detection of HIV-1 RNase H activity and inhibition by using graphene oxide (GO) as sensing platform. In our approach, a DNA-RNA substrate was prepared which a carboxy fluorescein (FAM) was labeled
at the 3 termini of single strand DNA. The FAM labeled DNA-RNA substrate preserves most of the fluorescence when mixed with GO. However, in the present of RNase H, RNase H can cleavage the RNA into fragments, resulting in the dissociation of ssDNA and mononucleotides. The fluorescence intensity
was greatly quenched after the addition of GO. Because of the super quenching ability of GO, the as-proposed method provides a wide linear range from 1.0 to 26.3 units mL–1 and a low detection limit of 0.6 units mL–1 for HIV-1 RNase H activity analysis. Additionally,
this approach shows potential for high-throughput screening of HIV-1 RNase H inhibitors. The method not only provides a universal platform for monitoring activity and inhibition of RNase H but also shows great potential in biological process researches, drug discovery, and clinic diagnostics.
研究领域:
生物技术
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分子生物学
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纳米技术
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病毒学
·
药物发现
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DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2025
·
IF 7.9
Haining Wu,Jiangchao Chen,Wenyu Zheng,Yiran Bai,Honghua Liang,Dazhi Pang,Anhong Luo,Yunyun Zhao,Huarui Xu,Kunpeng Jiang,Guisheng Zhu
Solid-state lithium metal batteries are considered as the next generation of high-energy-density batteries. However, the internal inorganic solid-state electrolyte in them suffers from issues such as low ionic conductivity, poor interfacial properties, and high production cost, which seriously hampers its commercialization. In this study, a copper-based metal-organic framework material (HKUST-1) is introduced with cellulose acetate as the polymer substrate. HKUST-1 possesses a controllable porous structure. Moreover, the unsaturated Cu 2+ within it disrupts the dense macro-hydrogen bonding inside cellulose, thereby giving rise to a cellulose acetate/LiTFSI/HKUST-1 quasi-solid-state electrolyte (CLH-X QSE) with multiple ion-transport channels. It exhibits high ionic conductivity (1.75 × 10 −3 S cm −1 ) at 30 °C. Additionally, it has excellent interfacial stability and can be stably cycled at room temperature for more than 2000 h. Meanwhile, the capacity retention of the LiFePO 4 /CLH-10-LiPF 6 /Li battery can still reach 97.29 % after 200 cycles at a current density of 0.2 C. This high-performance and inexpensive quasi-solid electrolyte furnishes inspiration for the development of next-generation energy storage devices and is anticipated to drive technological advancements and industrial upgrades throughout the battery industry.
研究领域:
材料科学
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能源存储技术
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产品关联
DOI
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2022
·
IF 3.858
Ling He,Wenjie Ye,Youyu Xie,Qinghai Liu,Hualei Wang,Dongzhi Wei
Bioreductions catalyzed by ketoreductases play an important role in the synthesis of chiral alcohols. However, the synthesis of (R)-2-chloro-1-(3,4-difluorophenyl)ethanol (24b), an important chiral intermediate for the synthesis of the anticoagulant ticagrelor, poses significant challenges in terms of high substrate concentration requirements that limit its production. In this study, a novel NADH-dependent, short-chain dehydrogenase, PpKR8, from Paraburkholderia phymatum STM815, exhibited excellent enantioselectivity and high activity for the production of (R)-CFPL (24b) through the reduction of 2-chloro-1-(3,4-difluorophenyl)ethanone (24a). The coexpression of PpKR8 and glucose dehydrogenase from Bacillus subtilis in Escherichia coli allowed up to 300 g/L (1.57 M) CFPO (24a) to be completely converted into 24b with 99.9% enantiomeric excess and a high space–time yield (728 g/(L day)). Substrate specificity assays demonstrated a broad substrate spectrum for PpKR8, which included 35 α/β-ketoesters, aromatic ketones, and heterocyclic ketones. Moreover, three additional optically pure chiral alcohols that are used as important drug intermediates were synthesized at high substrate concentrations (150–330 g/L), demonstrating the excellent industrial potential of PpKR8-mediated bioreductions.
研究领域:
生物化学
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酶工程
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制药工业
✓ 已验证
DOI
高影响因子
FUEL
·
2016
·
IF 3.611
Qi Du,Yiping Guo,Huanan Duan,Hua Li,Yujie Chen,Hezhou Liu
Hierarchical TS-1 (HTS-1) zeolite has been successfully synthesized through a novel three-step crystallization method by using a cheap and eco-friendly polymer PVA as the mesopore template. The HTS-1 particles possess an interesting stacking structure and are constructed of smaller crystals with an average diameter of 30 nm. The mesopores of HTS-1 are formed by the stacking of the smaller TS-1 nanocrystals . The catalytic results show that the HTS-1 exhibits much higher catalytic activity than conventional TS-1 in the oxidative desulfurization of dibenzothiophene (DBT) and 4,6-dimethyldibenzothiophene (4,6-DMDBT) due to the presence of intracrystal mesopores. In addition, the three-step crystallization method plays a key role in the preparation of HTS-1, which effectively solves the problem that PVA is unstable and unsuitable to be used as mesopore template under high crystallization temperature .
研究领域:
材料化学
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催化化学
·
纳米材料科学
·
环境化学
·
能源化工
✓ 已验证
DOI
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2020
·
IF 3.943
Jingyuan Wan,Ge Kuang,Li Zhang,Rong Jiang,Yongtao Chen,Zhen He,Duyun Ye
Acetaminophen (APAP) is a common antipyretic and analgesic drug, but its overdose can induce acute liver failure with lack of effective therapies. Hesperetin, a dihydrogen flavonoid compound, has been revealed to exert multiple pharmacological activities. Here, we explored the protective effects and mechanism of hesperetin on APAP-induced hepatotoxicity. The results showed that pretreatment with hesperetin dose-dependently attenuated APAP-induced acute liver injury in mice, as measured by alleviated serum enzymes activities, hepatic pathological damage and apoptosis. Moreover, hesperetin mitigated APAP-induced oxidative stress and inflammatory response in mice by inhibiting oxidative molecules but increasing antioxidative molecules production, reducing inflammatory cells infiltration and proinflammatory cytokines production, blocking Toll-like receptor (TLR)-4 signal activation. In vitro experiment indicated that hesperetin dose-dependently inhibited APAP-primed cytotoxicity, apoptosis, and reactive oxygen species (ROS) in murine AML12 hepatocytes. Notably, hesperetin up-regulated expression of heme oxygenase-1 (HO-1) mRNA and protein in the liver of mice and AML12 cells exposed to APAP. Furthermore, knockdown of HO-1 by adenovirus-mediated HO-1 siRNA reverted these beneficial effects of hesperetin on APAP-induced hepatocytotoxicity as well as ROS and inflammatory response in vivo and in vitro . These findings demonstrated that hesperetin exerted a protective prophylaxis on APAP-induced acute liver injury by inhibiting hepatocyte necrosis and apoptosis, oxidative stress and inflammatory response via up-regulating HO-1 expression.
研究领域:
药理学
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肝脏病学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy
·
2024
·
IF 1.4
Yao Xinyu,Shu Yang,Xu Lei,Wei Lijuan,Cui Minhe,Li Li,Zhang Peng,Fang Tianqi
Colistin is recognized as the “last line of defense” for the treatment of carbapenemase-producing “super-resistant” bacteria. However, the treatment of clinical bacterial infections has become more challenging due to the widespread prevalence of the novel resistance gene mcr-1 in multidrug-resistant pathogens. Currently, the use of natural compounds as novel adjuvants to colistin treatment is a safe, feasible, and promising approach compared to the high cost and unpredictability of developing novel antimicrobial drugs. Here, we identified a potential MCR-1 inhibitor, ursodeoxycholic acid, that may be used to treat infections caused by MCR-1-positive drug-resistant bacteria. In this study, checkerboard minimum inhibitory concentration assays demonstrated that ursodeoxycholic acid could reduce the MICs of colistin-resistant strains 4–64 times. Furthermore, a time-kill curve analysis, combined with disk diffusion testing and live/dead bacterial staining, demonstrated that ursodeoxycholic acid could effectively enhance the antibacterial activity of colistin, and when the concentration of ursodeoxycholic acid was lower than 512 μg/ml, it did not affect the normal growth of bacteria; thus, ursodeoxycholic acid imparts less selective pressure on bacteria and did not easily induce the emergence of drug resistance. In addition, through potential target prediction, molecular docking, RT-PCR analysis, membrane permeability detection, and scanning electron microscopy analysis, we demonstrated that ursodeoxycholic acid can form a complex with MCR-1, suggesting that UDCA may affect the activity of MCR-1. In addition, ursodeoxycholic acid combined with colistin was able to alter the electronegativity and permeability of the bacterial membrane, thereby disrupting the structural integrity of the bacterial membranes and significantly enhancing membrane damage–associated colistin activity. In summary, our findings indicate that the colistin/ursodeoxycholic acid combination may be a promising alternative approach for treating MCR-1-positive bacterial infections. Graphical Abstract
研究领域:
微生物学
·
药理学
·
传染病学
·
分子生物学
✓ 已验证
DOI
高影响因子
Nutrition & Diabetes
·
2024
·Vol. 14
·期 1
·1-9
·
IF 4.6
Li Hui,Fang Yujiao,Wang Da,Shi Bowen,Thompson Garth J.
Background Quantitative mapping of the brain’s metabolism is a critical tool in studying and diagnosing many conditions, from obesity to neurodegenerative diseases. In particular, noninvasive approaches are urgently required. Recently, there have been promising drug development approaches for the treatment of disorders related to glucose metabolism in the brain and, therefore, against obesity-associated diseases. One of the most important drug targets to emerge has been the Glucagon-like peptide-1 (GLP-1) and its receptor (GLP-1R). GLP and GLP-1R play an important role in regulating blood sugar and maintaining energy homeostasis. However, the macroscopic effects on brain metabolism and function due to the presence of GLP-1R are unclear. Methods To explore the physiological role of GLP-1R in mouse brain glucose metabolism, and its relationship to brain function, we used three methods. We used deuterium magnetic resonance spectroscopy (DMRS) to provide quantitative information about metabolic flux, fluorodeoxyglucose positron emission tomography (FDG-PET) to measure brain glucose metabolism, and resting state-functional MRI (rs-fMRI) to measure brain functional connectivity. We used these methods in both mice with complete GLP-1R knockout (GLP-1R KO) and wild-type C57BL/6N (WT) mice. Results The metabolic rate of GLP-1R KO mice was significantly slower than that of WT mice ( p = 0.0345, WT mice 0.02335 ± 0.057 mM/min, GLP-1R KO mice 0.01998 ± 0.07 mM/min). Quantification of the mean [ 18 F]FDG signal in the whole brain also showed significantly reduced glucose uptake in GLP-1R KO mice versus control mice ( p = 0.0314). Observing rs-fMRI, the functional brain connectivity in GLP-1R KO mice was significantly lower than that in the WT group ( p = 0.0032 for gFCD, p = 0.0002 for whole-brain correlation, p < 0.0001 for ALFF). Conclusions GLP-1R KO mice exhibit impaired brain glucose metabolism to high doses of exogenous glucose, and they also have reduced functional connectivity. This suggests that the GLP-1R KO mouse model may serve as a model for correlated metabolic and functional connectivity loss.
研究领域:
神经科学
·
代谢疾病
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药理学
·
成像技术
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Feng Lin,Hong Huang,Jie Long,Renchuan Liang,Yunxi Huang,Xiaoling Luo
Cancer remains a major global health burden due to its high rates of recurrence and resistance to conventional therapies. Although PD-L1/PD-1 immune checkpoint inhibitors (ICIs) have emerged as promising treatments by restoring T cell function and enhancing anti-tumor immunity, their efficacy is often limited by immunosuppressive tumor microenvironment (ITM) and metabolic adaptations that impair T cell activity. To address these challenges, we developed a novel intelligent drug delivery platform that integrates PANoptosis induction, metabolic modulation and targeted nanotherapy. Specifically, hollow mesoporous manganese dioxide (H-MnO 2 ) nanoshells were synthesized and co-loaded with SOAT1 inhibitor avasimibe (Ava). Trop2-specific targeting ligands were further conjugated to enable precise tumor localization, resulting in the multifunctional nanoplatform Ava@HM/Trop2. Upon tumor accumulation via Trop2-mediated targeting, acidic pH conditions and TME stimuli trigger rapid degradation of the H-MnO 2 nanoshells, releasing Ava and inducing PANoptosis. This process activates the cGAS-STING pathway, remodels immunosuppressive TME, mitigates lipid-induced T cell senescence, and synergizes with PD-1 blockade to potentiate anti-tumor immunity. Both in vitro and in vivo experiments demonstrate that Ava@HM/Trop2 achieves efficient tumor targeting, robust tumor cell apoptosis, and improved therapeutic outcomes. This innovative multi-modal strategy highlights a promising avenue for overcoming immunotherapy resistance and advancing the clinical management of solid tumors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLUTION CHEMISTRY
·
2018
·Vol. 47
·期 6
·1037-1047
·
IF 1.401
Gao Jing,Liu June,Wei Yuanyi,Nie Fanghong,Ji Hongwu
Ionic liquids (ILs) based aqueous biphasic systems (ABSs) have been successfully applied to the extraction and purification of biomolecules. Although much research has focused on the effect of ILs on the phase formation, there are few reports that describe the phase behavior of quaternary IL-based ABS systems using amino acids (AAs) as additives. Here, 1-butyl-3-methylimidazolium tetrafluoroborate ([C 4 mim]BF 4 ) formed an ABS with sodium citrate (Na 3 C 6 H 5 O 7 ) with the aid of AAs. The phase behavior and physical properties of the ABS were investigated at 298 K. The capacity of the AA to create the ABS (area of biphasic region) increases with increasing in solubility and decrease with the molecular weight of the ILs and follows the trend glycine > l -alanine ≈ l -lysine > l -threonine > l -proline ≈ l -arginine > none. Moreover, the pH of the top and bottom phases containing l -proline as additive are nearly neutral; l -threonine, glycine, and l -alanine result in mildly acidic environments, while l -arginine and l -lysine provide basic pH conditions. The results show that the effects of the addition of the AAs can be considerable and important in the simulation, design calculation and pH control of ABS for efficient separation and extraction processes.
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Science
·
2020
·Vol. 8
·期 8
·2212-2226
·
IF 6.183
Xue Feng,Tong Zhou,Peifang Xu,Juan Ye,Zhongru Gou,Changyou Gao
Due to the poor self-repair capabilities of articular cartilage, chondral or osteochondral injuries are difficult to be recovered. In this study, an N-cadherin mimetic peptide sequence HAVDIGGGC (HAV) was conjugated to direct cell–cell interactions, and an aggrecanase-1 cleavable peptide sequence CRDTEGE-ARGSVIDRC (ACpep) was used to crosslink hyperbranched PEG-based multi-acrylate polymer (HBPEG) with cysteamine-modified chondroitin sulfate (Cys-CS), obtaining an aggrecanase-1 responsively degradable and HAV-conjugated hydrogel ((HAV-HBPEG)-CS-ACpep). A HBPEG-CS-ACpep hydrogel without the HAV motif was also prepared. The two hydrogels exhibited similar equilibrium swelling ratios, elastic moduli and pore sizes after lyophilization, indicating the negligible influence of conjugated HAV on the crosslinking networks and mechanical properties of the hydrogels. After being degraded in PBS, aggrecanase-1 (ADAMTS4) and trypsin, the HBPEG-CS-ACpep hydrogel exhibited significantly decreased elastic moduli with a much lower value when incubated in enzyme solutions. The two hydrogels could maintain the viability of encapsulated bone marrow-derived mesenchymal stem cells (BMSCs), and the (HAV-HBPEG)-CS-ACpep hydrogel better promoted the cell–cell interactions. After being implanted into osteochondral defects in rabbits for 18 weeks, the two cell-laden hydrogel groups achieved better repair effects than the blank control group. Moreover, hyaline cartilage was formed in the (HAV-HBPEG)-CS-ACpep/BMSCs hydrogel group, while a hybrid of hyaline cartilage and fibrocartilage was found in the HBPEG-CS-ACpep/BMSCs hydrogel group.
研究领域:
组织工程
·
再生医学
·
生物材料
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2022
·
IF 3.119
Xiaopo Wang,Heyu Jia,Xiaojie Wang,Feridun Esmaeilzadeh
The liquid densities and viscosities of ethyl caprate/1-propanol mixture were investigated at ethyl caprate molar fractions of 0.25, 0.50, and 0.75 in this work. The measurements were carried out using a vibrating-tube densimeter and a falling-cylinder viscometer, and the studied temperature range was from 293 to 363 K, and the pressure was up to 60 MPa for density and 30 MPa for viscosity. The Tait equation was used to correlate the experimental densities. In addition, for comparison purpose, the modified Andrade equation and the Vogel–Fulcher–Tammann (VFT) equation were used to correlate the viscosities. For the density and viscosity of the mixtures at different molar fractions, the average absolute relative deviations between experimental and calculated values are all less than 0.01 and 0.47%, respectively.
研究领域:
化学工程
·
物理化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）