EXPERIMENTAL NEUROLOGY
·
2025
·
IF 4.2
Jun Fu,Chengjie Ding,Wenqing Li,Jiali Lei,He Wang,Lingfei Meng,Xiaoya Lin,Weiyuan Chen,Hongchang Gao
Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive cognitive decline, with hallmark pathological features including Aβ deposition and tau protein hyperphosphorylation. These pathological alterations are particularly prominent in the Prefrontal Cortex (PFC) and exert profound effects on cognitive function. The present study aimed to reveal key proteins in the PFC and provide insight into their potential mechanisms of action in AD. Accordingly, proteomic analysis was conducted to profile differential proteins in 5 × FAD mice, followed by in vivo and in vitro validation of core proteins through AAV-mediated interference of target gene expression. Furthermore, transcriptomics and molecular biology techniques were employed to confirm the underlying mechanisms of action of core proteins in AD. The results demonstrated that ELAVL1 was significantly upregulated in the PFC of AD mice. Behavioral experiments revealed that inhibition of ELAVL1 expression markedly improved long-term memory in AD mice. Immunostaining and Western blot analyses confirmed that suppressing ELAVL1 expression alleviated Aβ1–42 deposition and apoptosis. In vitro experiments indicated that knocking down ELAVL1 significantly reduced the apoptotic response of SH-SY5Y cells induced by Aβ1–42 and APP/Swe cells. Transcriptomic analysis further revealed that ELAVL1 regulates Aβ1–42-induced apoptosis via activation of the Bcl-2/Bax pathway. Notably, immunofluorescence co-localization staining and pull-down assays confirmed that ELAVL1 directly interacts with APP. Collectively, these findings provide valuable insights into the molecular mechanisms underlying AD and suggest ELAVL1 as a promising therapeutic target for AD.
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DOI
PubMed
高影响因子
MATERIALS LETTERS
·
2012
·
IF 2.307
Chang Gao,Yurong Cai,Xiangdong Kong,Guoxiang Han,Juming Yao
The injectable chitosan/β-glycerophosphate (CS/GP) hydrogels were prepared with dexamethasone (Dex)/recombinant human bone morphogenetic protein (rhBMP-2) loaded hydroxyapatite nanoparticles (nHAP). The obtained samples were characterized by TEM, SEM, XRD and nitrogen adsorption tests, and the effect of Dex and rhBMP-2 on the mesenchymal stem cells (MSCs) was evaluated. The results showed that nHAP could be dispersed homogeneously in the matrix of injectable CS/GP hydrogels and the combination of Dex and rhBMP-2 could promote the proliferation and osteoblastic differentiation of MSCs, indicating that the prepared injectable CS/GP hydrogels with Dex and rhBMP-2 loaded nHAP have great potential in different orthopedic regenerative applications.
研究领域:
生物材料学
·
再生医学
·
骨科
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DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2016
·Vol. 41
·期 39
·17462-17470
·
IF 3.205
Bing Zhai,Xuezhang Xiao,Wenping Lin,Xu Huang,Xiulin Fan,Shouquan Li,Hongwei Ge,Qidong Wang,Lixin Chen
NbF 5 and eutectic 0.68LiBH 4 –0.32Ca(BH 4 ) 2 were successively infiltrated into highly ordered mesoporous carbon (CMK-3) to obtain a LiBH 4 –Ca(BH 4 ) 2 –NbF 5 @CMK-3 composite. A synergetic effect of nanoconfinement and catalysis on hydrogen desorption properties of the composite was achieved. The onset desorption temperature of LiBH 4 –Ca(BH 4 ) 2 –NbF 5 @CMK-3 is dramatically lower than 120 °C compared with that of as-milled LiBH 4 –Ca(BH 4 ) 2 or LiBH 4 –Ca(BH 4 ) 2 @CMK-3. A hydrogen amount as high as 13.3 wt% can be released within 250 min for LiBH 4 –Ca(BH 4 ) 2 –NbF 5 @CMK-3, compared with only 10.4 wt% for pure LiBH 4 –Ca(BH 4 ) 2 , in which nanoconfinement and catalysis both make some contribution. The cycling hydrogen desorption performance of the composite becomes better likewise due to the addition of NbF 5 . Further microstructure analysis reveals that F − tended to bind with Ca 2+ to form CaF 2 , whereas Nb compound is believed to catalyze the whole system in the form of Nb-based boride (NbB 2 ).
研究领域:
材料科学
·
能源化学
·
纳米科技
·
储氢材料
·
物理化学
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DOI
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2024
·
IF 3.3
Nan Wang,Yuanyuan Huang,You Zi,Mengke Wang,Weichun Huang
A practical and regioselective direct N-alkylation of 2-pyridones is enabled by use of α-keto esters in the P(NMe2)3-mediated deoxygenation process. The reaction proceeds under mild conditions to produce N-alkylated 2-pyridones with high selectivity and generality, and the protocol is shown to be applicable for the scale-up synthesis, which makes it promising for practical applications.
研究领域:
有机化学
·
合成化学
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DOI
PubMed
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2019
·Vol. 33
·期 6
·e4815
·
IF 3.259
Qiancheng Zou,Yi Wei,Shuai Wang,Jinyan Xie,Aihua Xu,Xiaoxia Li
The development of green and efficient catalysts for peroxymonosulfate (PMS) activation and organic pollutants degradation has received widespread attention. In this study, the hybrid CaCO 3 /OMS-2 catalysts were prepared by a simple precipitation approach and characterized by X-ray powder diffraction, N 2 adsorption–desorption, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and cyclic voltammetry. It was found that deposition of CaCO 3 on OMS-2 surface can weaken the Mn-O bond by formation of Ca-O-Mn bond. The interactions between CaCO 3 and OMS-2 significantly enhanced Acid Orange 7 degradation in the presence of PMS with a pseudo-first-order kinetic constant of 0.21 min −1 , which was much higher than those of OMS-2 (0.026 min −1 ) and CaCO 3 (0.021 min −1 ). The CaCO 3 /OMS catalysts were also much more efficient than other reported OMS-2 hybrid catalysts, and could be performed over a wide solution pH and for other organic dyes degradation. Sulfate and hydroxyl radicals were formed from the oxidation of low valent manganese species by PMS as the active species in the system. This study can provide a simple method for the design of efficient manganese-based hybrids for wastewater remediation via PMS activation.
研究领域:
环境科学
·
催化化学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Mengdie Zhou,Pan Wang,Yulin Song,Hao Li,Jianquan Luo,Jianming Pan
Hydrogel microspheres encapsulating molecularly imprinted polymers (MIPs) are promising hybrid sorbents, due to several advantages of high selectivity, fast mass transfer efficiency, and simple collection. Thus, Janus single-hole hollow nanoparticles (J-HNPs) with the size of 550 ± 70 nm were firstly designed by anisotropic emulsion template, and then MIPs were grafted onto their inner surface through electron transfer atom transfer radical polymerization (ARGET ATRP). Then as-prepared J-HNPs-MIPs were loaded into hydrogel microspheres via polymerizable water-in-oil (W/O) emulsion droplets combining gelatin methacryloyl (GMA) as monomers, and then obtained J-HNPs-MIPs@Gel with the mean diameter of 2.0 µm was applied for effective and selective separation of 2′-deoxyadenosine (dA). Fast adsorption equilibrium of J-HNPs-MIPs@Gel for dA can be achieved within 40 min, thanks to the hydrogel matrix and single-hole hollow structure for enhancing diffusion. The maximum multi-layer adsorption capacity calculated according to the Freundlich model was 10.31 μmol g −1 at 298 K. The specific memory to the size, shape and functional groups of dA endowed excellent recognition ability, and 88% of the initial capacity after four consecutive adsorption-desorption cycles was maintained. In addition, J-HNPs-MIPs@Gel was expected to show great potential for the selective enrichment and analysis of target dA molecule in complex biological samples.
研究领域:
生物化学
·
材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Fei Li,Yunlu Wei,Jing Zhao,Guoyong Yu,Linlin Huang,Quanhong Li
Pumpkin polysaccharides with various bioactivities are mainly taken orally, thus detailed knowledge of the intestinal transport of which are essential for understanding its bioactivities. The Caco-2 cells monolayer model (mimic intestinal epithelium) was successfully constructed and Cucurbia moschata polysaccharides (PPc-F) were successfully conjugated with fluorescein isothiocyanate (FITC) to evaluate the transcellular transport mechanism and subcellular localization of PPc. The transport process of PPc-F was energy-dependent, and a moderately-absorbed biological macromolecule according to the apparent permeability coefficients (P app ) value. The endocytosis process of PPc-F in Caco-2 cells included the clathrin- and caveolae (or lipid draft)-medicated routes. And the translocation process was related to endoplasmic reticulum (ER), golgi apparatus (GA), tubulin and the acidification of endosomes. As for the intracellular location of PPc-F, it was mainly accumulated in ER. The study provided an understanding of the transmembrane transport of PPc-F, and could help studying the mechanisms of its effects.
研究领域:
药理学
·
分子生物学
·
营养科学
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DOI
PubMed
高影响因子
INORGANICA CHIMICA ACTA
·
2021
·
IF 2.545
Xingliang Li,Juncai Lu,Wanjun Mu,Baihua Chen,Daibing Luo,Bijun Liu,Yanqiu Yang,Hongyuan Wei,Shuming Peng
Nitrilotri–3–propanoic Acid (NTPA) and Tris(2–carboxyethyl) phosphine (TCEP) possess similar skeleton. This pair of isostructural ligands is a perfect set to assess the differences of uranyl (UO 2 2+ ) complexes with N–tricarboxylate versus P–tricarboxylate. The protonation reactions of NTPA and TCEP were investigated by potentiometry and calorimetry. Complexation of uranyl with the both ligands was studied by potentiometry and structural analysis. The results indicate that central trialkylamine in NTPA has a higher affinity for the proton than trialkylphosphine in TCEP. Crystal structures and NMR analyses indicate that central amine of NTPA and central phosphine of TCEP are not involved in the bonding. NTPA/UO 2 2+ complex is more stable than TCEP/UO 2 2+ complex. This difference cannot be explained by electronic effect of central N and P atoms because of nonbonding interactions. The higher stability of UO 2 2+ /NTPA versus UO 2 2+ /TCEP may be due to the fact that the central N atom of NTPA has a lower steric hindrance than the central P atom of TCEP. Another possible reason is that UO 2 2+ /TCEP complexes have different coordination modes from UO 2 2+ /NTPA complexes.
研究领域:
化学
·
配位化学
·
核化学
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DOI
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2021
·Vol. 50
·期 12
·6772-6783
·
IF 1.938
Hou Xiaoshuo,Yang Ming,Yu Hui,Yang Ying,Li Dan,Dong Xiangting
In this work, a 3D Z-scheme photocatalyst Ag/AgBr/Zn 3 V 2 O 7 (OH) 2 ·2H 2 O (Ag/AgBr/ZVO) was synthesized by a hydrothermal method, and a subsequent surface deposition-optical reduction technique. The prepared flower-like Ag/AgBr/ZVO was characterized by scanning election microscopy (SEM), x-ray powder diffraction (XRD), x-ray photoelectron spectroscopy (XPS), UV-vis spectrophotometry, N 2 adsorption-desorption isotherm based on Brunauer-Emmett-Teller (BET) theory, and electrochemical tests. The photocatalytic degradation performance of the catalyst was investigated by the degradation of methylene blue (MB) dye by visible light irradiation. Compared with bare Zn 3 V 2 O 7 (OH) 2 ·2H 2 O (ZVO) nanoflowers, the photocatalytic effect of the 3D Z-scheme heterojunction Ag/AgBr/ZVO was significantly enhanced. The catalyst of 2h-Ag/AgBr/ZVO exhibited the strongest photocatalytic activity, of which the MB degradation rate is about 98.8% in 100 min, which was about five times that of pure ZVO. The increase in the photodegradation rate is ascribed to the accelerated interface charge-transfer process, the faster photogenerated carrier separation rate and the enhancement of visible light absorption. In view of the trapping of the reaction intermediate and the electron spin resonance (ESR) measurement, we propose a possible Z-scheme photocatalytic mechanism. This improved visible-light response likely originates from a synergistic effect of the different components. This work offered an ideal method for producing stable, efficient, and recyclable photocatalysts suitable for use in the visible light range.
研究领域:
材料科学
·
环境科学
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DOI
高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Fangqin Xiang,Yuxing Li,Yumeng Zhang,Yuning Yang,Bailiang Li,Sufang Duan
Ulcerative colitis (UC) is a chronic disease with an increasing incidence worldwide. 2’-Fucosyllactose (2’-FL) is the most abundant fucosylated neutral human milk oligosaccharides (HMOs), accounting for approximately 31% of the total HMOs content, and shows considerable potential in alleviating UC. This study aimed to investigate the therapeutic effect of 2’-FL on DSS-induced colitis in mice and explore its potential mechanisms. The results showed that HMO alleviated the symptoms of DSS-induced colitis in mice by alleviating weight loss, reducing the disease activity index (DAI), increasing the length of the mouse colon and improving the spleen index; HMO enhanced intestinal barrier function by repairing colon tissue damage, reducing intestinal permeability (DAO and D-LA), increasing the expression of Tight junctions (TJs), promoting the production of anti-inflammatory factor IL-10, and inhibiting cytokines (IL-1β, IL-6, and TNF-α). In addition, the activation of the colitis-related NF-κB pathway was significantly inhibited after HMO intervention. Furthermore, metagenomic analysis revealed that HMO intervention in colitis mice significantly increased the relative abundance of Bacteroidetes, Parabacteroidetes, Parabacteroides and Prevotella .; non-targeted metabolomics analysis demonstrated that HMO modulated the host's bile acid metabolism by preserving the level of deoxycholic acid. To further investigate the molecular mechanisms affecting bile acid metabolism, enzyme scanning was performed based on species-level genome enriched in different groups. The results showed that the bacteria mainly enriched in the HMO treatment group showed greater potential in encoding bile salt hydrolases (BSH) responsible for the dissociation of primary bile acids and 7α-hydroxysteroid dehydrogenases (7α-HSDs) that produce secondary bile acids. In summary, this study showed that HMO intervention changed the composition of the intestinal flora, and the specially enriched intestinal microorganisms regulated the host's secondary bile acid metabolism, thereby effectively alleviating colon inflammation in mice.
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Jiafeng Ding,Gaoyuan Long,Yang Luo,Runze Sun,Mengxia Chen,Yajun Li,Yanfang Zhou,Xinhua Xu,Weirong Zhao
Polychlorinated dibenzo- p -dioxins (PCDDs), as a group of notorious anthropogenic environmental toxicants, are arguably ubiquitous in nature. In this study, we investigated the photocatalytic reductive dechlorination of 2-chlorodibenzo- p -dioxin (2-CDD) over Pd/g-C 3 N 4 catalysts under UV–vis irradiation. The g-C 3 N 4 and a series of Pd/g-C 3 N 4 catalysts were prepared by thermal polymerization and mechanical mixing-illumination method and characterized by XRD, TEM, BET, SEM and UV–vis DRS analyses. Among all the samples, the Pd/g-C 3 N 4 (5 wt%) yielded the optimal dechlorination activity with a total 2-CDD conversion of 54% within 4 h, and 76% of those converted 2-CDD were evolved to dibenzo- p -dioxin (DD). The kinetics of dechlorination could be described as pseudo-first-order decay model (R 2 > 0.84). Corresponding rate constants (k) increased from 0.052 to 0.17 h −1 with Pd contents up to 5 wt% and decreased to 0.13 h −1 with a 10 wt% of Pd. The enhanced activities originated from the surface plasmonic resonance (SPR) effect of Pd nanoparticles and the formation of Schottky barrier between Pd and g-C 3 N 4 , which extend the spectrum responsive range and suppress the charge recombination of g-C 3 N 4 . This is the first report on the photocatalytic reductive removal of PCDDs and may provide a new approach for PCDDs pollution control.
研究领域:
环境科学
·
化学工程
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2024
·
IF 2
Pengjun Yao,Farao Zhang,Yang Yu
In this paper, the mole fraction solubility of 2-hydrazino-4-methylbenzothiazole (MHB) in acetone, toluene, methanol, ethanol, water, ethyl acetate, n-propanol, isopropanol, 1-butanol, and methanol/ethanol/n-propanol/acetone + water was determined by the isothermal saturation method at 273.15 to 318.15 K under 101.2 kPa. Meanwhile, high-performance liquid chromatography was used to detect the solubility of MHB. The mole fraction of MHB increased with the increase of temperature in monosolvents, and the composition of solvent mixtures also played a decisive role in the solubility of MHB in mixed systems. Then, four models for five monosolvents (Apelblat, van’t Hoff, λh, NRTL, and the Jouyban model) and three models for mixed solvents [Jouyban–Acree (J–A), van’t Hoff–Jouyban–Acree (V–J–A), and modified Apelblat–Jouyban–Acree (A–J–A) model] were utilized to correlate the mole fraction of MHB. The maximum deviations [relative average deviation (RAD) and root-mean-square deviation (RMSD)] between the experimental data and the solubility data calculated by the models for monosolvents were 7.13% and 6.11 × 10–4 (from the Jouyban model in pure ethyl acetate), respectively. The maximum RAD and RMSD for mixed systems were 1.58% (from the A–J–A model in methanol + water) and 1.35 × 10–4 (from the J–A and A–J–A models in acetone + water), respectively.
研究领域:
化学工程
·
药物化学
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DOI
高影响因子
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