MUSCLE & NERVE
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2025
·Vol. 71
·期 3
·474-486
·
IF 3.1
Zhuajin Bi,Qing Zhang,Huajie Gao,Huizhen Ge,Jiayang Zhan,Mengge Yang,Bitao Bu
Introduction/Aims Tofacitinib, a first-generation Janus kinase (JAK) 1/3 inhibitor, is commonly used for treating ulcerative colitis and rheumatoid arthritis. However, its role in myasthenia gravis (MG) remains unclear. This study aimed to evaluate the immunomodulatory effects of tofacitinib on experimental autoimmune myasthenia gravis (EAMG) and peripheral blood mononuclear cells (PBMCs) from patients with MG. Methods Flow cytometry, enzyme-linked immunosorbent assay (ELISA), quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western blot were used to evaluate the effects of tofacitinib on T helper (Th) cell profiles, humoral immune responses, and the JAK-signal transducer and activator of transcription (STAT) pathway proteins. Results In vivo, tofacitinib significantly ameliorated EAMG severity in rats, reducing the proportions of Th1, Th17 and memory B cells, and anti-acetylcholine receptor (AChR) antibodies levels, while increasing the proportions of regulatory T (Treg) cells. In vitro, tofacitinib administration resulted in a significant decrease in the proportions of Th1 and IgG-secreting B cell, and a significant upregulation of Treg cells in mononuclear cells (MNCs) from EAMG rats, which was consistent with findings in PBMCs from MG patients. Further analysis revealed that tofacitinib inhibited CD4 + T cell differentiation into Th1 by decreasing phosphorylated STAT1 levels, while promoting Treg differentiation via increased phosphorylated STAT5 levels in MNCs from EAMG rats. Discussion Tofacitinib modulates Th cell profiles and humoral immune responses by targeting the JAK–STAT pathway, suggesting its potential as a therapeutic candidate for MG. Further clinical studies are warranted to evaluate the efficacy and safety of tofacitinib in MG patients.
研究领域:
免疫学
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药理学
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神经病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Hongjun Zang,Yimo Feng,Jing Lou,Kai Wang,Changchun Wu,Zhipeng Liu,Xi Zhu
Ionic liquids have shown good catalytic performance and been widely used in the biomass conversion process due to their unique properties, including environmental friendship, low toxicity, and simple preparation. In this work, a series of novel piperidinium-based ionic liquids were synthesized under mild conditions via a simple protonation reaction or nucleophilic substitution reaction. Among them, 4,4′-(propane-1,3-diyl)bis(1-(carboxymethyl)piperidin-1-ium) chloride ([PDCMPi]Cl) displayed the best catalytic performance. The highest yield (42.81 %) of 3A5AF from NAG was obtained after reacting in N-methyl-pyrrolidone (NMP) in the presence of piperidinium-based ionic liquids at 180 °C for 20 min under normal atmospheric conditions. Additionally, ionic liquid [PDCMPi]Cl showed good catalytic activity in six repeated catalytic runs. Meanwhile, a possible reaction pathway was also proposed on the basis of the results of NMR spectra.
研究领域:
催化化学
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绿色化学
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生物质能源
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有机合成
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离子液体化学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 20
·5527-5535
·
IF 3.125
Lian Ziru,Li Hai-Bei,Wang Jiangtao
An innovative and effective extraction procedure based on molecularly imprinted solid-phase extraction (MISPE) was developed for the isolation of gonyautoxins 2,3 (GTX2,3) from Alexandrium minutum sample. Molecularly imprinted polymer microspheres were prepared by suspension polymerization and and were employed as sorbents for the solid-phase extraction of GTX2,3. An off-line MISPE protocol was optimized. Subsequently, the extract samples from A. minutum were analyzed. The results showed that the interference matrices in the extract were obviously cleaned up by MISPE procedures. This outcome enabled the direct extraction of GTX2,3 in A. minutum samples with extraction efficiency as high as 83 %, rather significantly, without any need for a cleanup step prior to the extraction. Furthermore, computational approach also provided direct evidences of the high selective isolation of GTX2,3 from the microalgal extracts.
研究领域:
海洋生物学
·
分析化学
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DOI
高影响因子
IONICS
·
2015
·Vol. 21
·期 9
·2503-2510
·
IF 1.754
Ma Xueli,Wang Chunfeng,Ren Hao,Chen Yujuan,Zhuo Kelei
1-Hexyl-3-methylimidazolium tetraphenylborate ([C 6 mim]-TPB) was synthesized and explored as ion exchanger in carbon paste electrode (CPE) as an ion-selective electrode (ISE). The electrode was found to be optimal with the composition of 42 % graphite powder, 20 % paraffin oil, 30 % ion exchanger, 5 % MWCNTs, and 3 % nano-silica. The as-prepared electrode exhibits a Nernstian response (59.2 mV per decade) to 1-hexyl-3-methylimidazolium cation in the concentration range from 1.0 × 10 −5 to 1.0 × 10 −2 mol kg −1 . The novel as-prepared MWCNTs/nano-silica/CPE was successfully applied in the detection of [C 6 mim] + in distilled water, tap water, and river water with satisfactory results.
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产品关联
DOI
高影响因子
Advanced Composites and Hybrid Materials
·
2025
·Vol. 8
·期 4
·286
·
IF 21.8
Mao Gaoqiang,Lu Jingfan,Cai Haiyan,Yu Wanjing,Tong Hui,Guo Xueyi,Zeng Leiying,Jiang Long
Increasing the operating voltage can significantly increase the energy density of LiCoO 2 , but it is accompanied by severe structural damage and interface degradation. Especially at a high voltage above 4.5 V, LiCoO 2 faces severe challenges, such as irreversible phase transition, lattice oxygen loss, and electrode–electrolyte interface evolution. Herein, a high-voltage LiCoO 2 cathode material with bulk doping and surface coating synergistic modification was designed and prepared. The doped Mg 2+ ions entered the lithium layers as pillars, stabilizing the layered framework. Meanwhile, Li 1.3 La 0.3 Ti 1.7 (PO 4 ) 3 was uniformly coated on the surface of LiCoO 2 , which is beneficial for suppressing Co 3+/2+ ion leaching and interface side reactions during cycling. Furthermore, Li 1.3 La 0.3 Ti 1.7 (PO 4 ) 3 is a fast ion conductor, which facilitates the rapid transport of lithium ions on the surface of the cathode material. Owing to the synergistic effect of Mg 2+ ion doping and Li 1.3 La 0.3 Ti 1.7 (PO 4 ) 3 coating, the orbitals of Co 3d and O 2p in LiCoO 2 crystal are changed, and the irreversible phase transition and oxygen loss are inhibited. The modified material exhibited an excellent electrochemical performance. A discharge capacity of 175.5 mAh·g −1 at 10 C rate, as well as a capacity retention of 90.14% after 200 cycles at 1 C rate, was achieved in the modified material. This work supplies a new method for developing high-voltage oxide cathodes through bulk and interfacial modification.
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产品关联
DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2015
·
IF 2.871
Yu-Cai He,Zhi-Cheng Tao,Yun Ding,Dan-Ping Zhang,Yin-Qin Wu,Yun Lu,Feng Liu,Yu-Feng Xue,Cheng Wang,Jian-He Xu
To avoid adding NAD + and effectively transform ethyl 4-chloro-3-oxobutanoate, the mixture of l -glutamine (200 mM) and d -xylose (250 mM) was added into in n -butyl acetate–water (10:90, v/v) biphasic system instead of NAD + for increasing the biocatalytic efficiency. To further improve the synthesis of optically pure ethyl ( R )-4-chloro-3-hydroxybutanoate (>99% ee ), β-cyclodextrin was also added into this reaction media, and ethyl ( R )-4-chloro-3-hydroxybutanoate (>99% ee ) could be effectively synthesized from 800 mM ethyl 4-chloro-3-oxobutanoate in the yield of 100% by whole-cells of recombinant E. coli CCZU-A13. Finally, the possible mechanism for improving the reductase activity by supplementation of l -glutamine, d -xylose and β-CD was proposed. In conclusion, this strategy has high potential for the effective biosynthesis of ethyl ( R )-4-chloro-3-hydroxybutanoate (>99% ee ).
研究领域:
生物化学
·
酶工程
·
合成生物学
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Yuxiang Chen,Xiangyue Liao,Peng Wang,Ji Chen,Xiaoqin Zhang,Xingqiao Wu,Sean C. Smith,Dunmin Lin,Xin Tan,Qiaoji Zheng
The stable three-dimensional framework and high operating voltage of sodium superionic conductor (NASICON)-type Na 3 V 2 (PO 4 ) 3 has the potential to work with long cycle life and high-rate performance; however, it suffers from the poor intrinsic electronic conductivity and low energy density . Herein, Ga 3+ is introduced into Na 3 V 2 (PO 4 ) 3 to activate the V 4+ /V 5+ redox couple at a high potential of 4.0 V for enhancing energy density of the materials (Na 3 V 2-x Ga x (PO 4 ) 3 ). After the partial substitution of Ga 3+ for V 3+ , three redox couples (V 2+ /V 3+ , V 3+ /V 4+ and V 4+ /V 5+ ) of V are reversibly converted in the voltage range of 1.4–4.2 V, suggesting multi-electrons (>2e − ) involved in the reversible reaction, and simultaneously the electronic conductivity of the materials is effectively enhanced. As a result, the cathode with x = 0.75 exhibits excellent electrochemical properties: in the voltage range of 2.2–4.2 V, delivering an initial capacity of 105 mAh/g at 1C with a capacity retention rate of 92.3% after 400 cycles, and providing a stable reversible capacity of 88.3 mAh/g at 40C; and in the voltage range of 1.4–4.2 V, presenting the reversible capacity 152.3 mAh/g at 1C (497.6 Wh kg −1 ), and cycling stably for 1000 cycles at 20C with a capacity decay of 0.02375% per cycle. It is found that the Na 3 V 2-x Ga x (PO 4 ) 3 cathodes possess the sodium storage mechanism of single-phase and bi-phase reactions. This investigation presents a useful strategy to enhance the energy density and cycling life of NASICON-structured polyanionic phosphates by activating high-potential V 4+ /V 5+ redox couple.
研究领域:
材料科学
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电化学
·
能源存储
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DOI
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 17
·175101
·
IF 3.874
Yanling Liu,Manman Tan,Chunjuan Fang,Xiaoyan Chen,Huan Liu,Ying Feng,Yujuan Zhang,Weiping Min
Tumor-specific targeted delivery is a major obstacle to clinical treatment of hepatocellular carcinoma (HCC). Here we have developed a novel multi-functional nanostructure GAL-GNR-siGPC-3, which consists of Galactose (GAL) as the HCC-targeting moiety, golden nanorods (GNR) as a framework to destroy tumor cells under laser irradiation, and siRNA of Glypican-3 (siGPC-3) which induce specifically gene silence of GPC-3 in HCC. Glypican-3 (GPC-3) gene is highly associated with HCC and is a new potential target for HCC therapy. On the other hand, Gal can specifically bind to the asialoglycoprotein receptor which is highly expressed on membrane of hepatoma cells. GAL and siGPC-3 can induce targeted silencing of GPC-3 gene in hepatoma cells. In vivo and in vitro results showed that GAL-GNR-siGPC-3 could significantly induce downregulation of GPC-3 gene and inhibit the progression of HCC. More notably, GAL-GNR-siGPC-3 could induce both GPC-3 gene silencing and photothermal effects, and the synergistic treatment of tumors was more effective than individual treatments. In summary, GAL-GNR-siGPC-3 achieved a synergistic outcome to the treatment of cancer, which opens up a new approach for the development of clinical therapies for HCC.
研究领域:
纳米技术
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癌症治疗
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分子生物学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2020
·Vol. 103
·期 10
·5664-5677
·
IF 3.502
Yao Ji,Yongbao Xiao,Shujing Huang,Weichao Wang
Fluoro-sulfo-phosphate (FPS) glass is of current interest as potential material for laser application due to its good glass-forming ability, thermal, and chemical stability as well as the complicated local environment for incorporated species. Herein, the physical and luminescent properties of Er 3+ and Yb 3+ /Er 3+ -doped FPS glasses vs S/F ratio are investigated comprehensively. The low melting temperature (750°C) leads to fewer ingredients evaporation and easier operation. The sulfate addition depolymerizes the structure of FPS glasses, leading to either monotonic or nonmonotonic variations of physical properties, while no deterioration in thermal and limited one in chemical stability is caused. The addition of sulfate also modifies the local structure around optical active species and thus, leading to higher emission cross section (1.52 × 10 −20 cm 2 ), effective linewidth (68.4 nm), figure of merit (5.61 × 10 −23 s cm 2 ), gain bandwidth (102.44 × 10 −27 cm 3 ), and energy-transfer microparameters (51.87 × 10 −39 cm 6 /s), implying high possibility to serve as 1.5 μm laser application.
研究领域:
材料科学
·
光学
·
激光物理
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry A
·
2025
·
IF 9.5
Junyu Chen,Xuedong Zhang,Feixiang Wu,Long Xie,Feng Li,Nina Wu,Zixuan Cao,Jinwen Zhao,Yuxuan Zhang,Xin He,Hongxia Gu,Jianyu Huang,Qiao Huang
Integrating conversion-type cathodes (such as iron-based fluorides (IBFs)) into sulfide all-solid-state batteries (ASSBs) significantly enhances energy density but faces challenges including unstable cathode/electrolyte interfaces. To mitigate these issues, a near-amorphous Li1.26Mg0.12Zr1.86(PO4)3 (LMZP) coating technology is developed to protect nano-sized FeF2 (n-FeF2) and micro-sized FeF3 (m-FeF3) cathodes from reacting with the sulfide electrolyte for the first time. The LMZP coating approach offers simplicity, low energy consumption and scalability. With the LMZP-coated cathodes and lithium–indium (Li–In) anode, the ASSBs achieve a discharge capacity of 310 mAh g−1 after 600 cycles and a maximum areal capacity of 2 mAh cm−2 for n-FeF2, and 301 mAh g−1 after 700 cycles with a maximum areal capacity of 3.1 mAh cm−2 for m-FeF3. When using a lithium (Li) metal anode, the ASSBs maintain a discharge capacity of 310 mAh g−1 after 300 cycles for n-FeF2 and 300 mAh g−1 after 600 cycles for m-FeF3, demonstrating excellent cycling stability and capacity retention. Notably, a discharge capacity of 280 mAh g−1 is sustained after 200 cycles for n-FeF2 and 301 mAh g−1 after 180 cycles for m-FeF3, with a reduced stack pressure (10 MPa). These results are unprecedented in IBF-based ASSBs, highlighting the potential of advanced coating strategies to enable scalable, high-performance metal fluoride cathodes in ASSBs with higher energy densities.
研究领域:
储能材料
·
电化学
·
固态电池技术
·
表面涂层工程
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 11
·3520
·
IF 4.927
Hongyu Zeng,Xiaoqing Zhang,Qianna Zhen,Yifan He,Haoran Wang,Yang Zhu,Qi Sun,Min Ding
A novel dual-template magnetic molecularly imprinted polymer (MMIP) was synthesized to extract normetanephrine (NMN), metanephrine (MN) and 3-methoxytyramine (3-MT) from spot urine samples. As the adsorbent of dispersive solid-phase extraction (d-SPE), the MMIP was prepared using dopamine and MN as dual templates, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the crosslinking reagent and magnetic nanoparticles as the magnetic core. NMN, MN, 3-MT and creatinine (Cr) in spot urine samples were selectively enriched by d-SPE and detected by HPLC-fluorescence detection/ultraviolet detection. The peak area (A) ratios of NMN, MN and 3-MT to Cr were used for the diagnosis of pheochromocytomas and paragangliomas (PPGLs). The results showed that the adsorption efficiencies of MMIP for target analytes were all higher than 89.0%, and the coefficient variation precisions of intra-assay and inter-assay for the analytes were within 4.9% and 6.3%, respectively. The recoveries of the analytes were from 93.2% to 112.8%. The MMIP was still functional within 14 days and could be reused at least seven times. The d-SPE and recommended solid-phase extraction (SPE) were both used to pretreat spot urine samples from 18 PPGLs patients and 22 healthy controls. The correlation coefficients of ANMN/ACrand AMN/ACrbetween d-SPE and SPE were both higher than 0.95. In addition, the areas under the receiver operator curves for spot urine ANMN/ACr, AMN/ACrand plasma free NMN and MN were 0.975, 0.773 and 0.990, 0.821, respectively, indicating the two methods had the similar performances. The d-SPE method took only 20 min, which was effective in clinical application.Keywords:magnetic molecularly imprinted polymer;normetanephrine;metanephrine;3-methoxytyramine;spot urine;PPGLs diagnosis
研究领域:
医学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xing-Yu Wang,Ping-Ping Liu,Hai-Jie Cai,Miao-Miao Li,Chun-Hui Deng,Yong Zhao,Zhao-Huan Zhang,Yong-Heng Zhu
Biomarkers detection of foodborne pathogens through gas sensors provides a promising method to ensure food safety. However, rapidly and accurately identifying ultra-low concentration bacterial biomarkers is still an urgent task. In this study, PdRh bimetallic-loaded α-Fe 2 O 3 nanospindles with sufficient exposed surface and high surface activity are designed to achieve sensitive detection of the Listeria monocytogene biomarker s : 3-hydroxy-2-butanone. α-Fe 2 O 3 nanospindles with high exposure surfaces were prepared by modulating the axial diameter ratio. Then PdRh bimetals were uniformly introduced to regulate the surface activity of α-Fe 2 O 3 nanospindles. Compared to pure Fe 2 O 3 , the result 1.5 wt% PdRh-Fe 2 O 3 nanospindle exhibits significantly improved sensitivity and selectivity in detecting 3-hydroxy-2-butanone, characterized by a reduced operating temperature from 240°C to 150 °C, high sensitivity (R a /R g = 148.4, 50 ppm), impressive response/recovery time (7/10 s), low detection limit (200 ppb), notable selectivity, and great stability over a test period exceeding 30 days. Mechanism studies through ex/in-situ characterization and density functional theory calculations (DFT) reveal that the dramatic sensing performance advancement is mainly due to the morphology optimization, bimetallic activation, and their synergistic effects, leading to the increased surface active species, enhanced oxygen mobility, and the reduced activation energy. Furthermore, high-sensitivity detection of Listeria monocytogenes in actual samples is also achieved by using 1.5 wt% PdRh-Fe 2 O 3 nanoneedles-based sensor, indicating its significant potential for non-destructive testing applications in the food industry and opening new avenues for the rapid detection of Listeria monocytogenes .
研究领域:
材料科学
·
食品科学
·
生物医学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）