BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Jiwei Wang,Jingjing Fu,Han Chen,Ali Wang,Yuting Ma,Hanrong Yan,Yuting Li,Dehong Yu,Fenglei Gao,Shibao Li
Accurate quantitative, in situ and temporal tracking imaging of tumor-associated miRNAs in living cells could provide a basis for cancer diagnosis and prognosis. In this strategy, a surface-enhanced Raman scattering (SERS)-fluorescence (FL) dual-spectral sensor (DSS) was constructed based on the nanoscale photophysical properties of AuNPs , mediated by functionalized DNA , to achieve rapid imaging of FL and accurate SERS quantification of intracellular miRNAs. The dual-spectrum sensor in the strategy is highly sensitive, specific and reproducibly stable. The LOD values of the dual spectra were 3.58 pM (SERS) as well as 11.8 pM (FL) with RSD values less than 2.69%. The bispectral sensor self-assembled into a trimer by the lapidation of Y-type DNA under the excitation of the target, generating a stable enhanced electric field coupling; and selected adenine located in the enhanced electric field as the reporter molecule, simplifying the labeling process and variables of the Raman reporter molecule, distinguishing it from other traditional methods. This strategy successfully achieved accurate tracking and quantification of miR-21 in cancer cells and showed good stability in the cells. The reported probes are potential tools for reliable monitoring of biomolecular dynamics in living cells.
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PubMed
高影响因子
Coatings
·
2023
·Vol. 13
·期 8
·1400
·
IF 3.4
Yuyan Peng,Li Jiang,Yifan Li,Xiaoping Yu
Soluble programmed death-ligand 1 (sPD-L1) levels vary widely among different stages of tumor development, so the direct quantification of sPD-L1 as a cancer biomarker is useful in cancer diagnosis, prognosis and therapeutic assessment. There is an urgent need for an sPD-L1 detection method with a broad detection range and high sensitivity for monitoring cancer progression and evaluating the effectiveness of immunotherapy in real time. Herein, we have reported an enzyme-free, label-free surface plasmon resonance imaging (SPRi) sensor based on an aptamer/sPD-L1/anti–PD-L1 sandwich structure with gold nanoparticle (AuNP) signal enhancement for the ultrasensitive quantitative measurement of sPD-L1 for the first time. The gold chip of the SPRi sensing platform was modified by DNA aptamers, sPD-L1 was specifically adsorbed on the surface of a DNA aptamer-modified gold chip and then coupled with anti–PD-L1. Thus, the detection of sPD-L1 at different concentrations was realized through the formation of an aptamer/sPD-L1/anti–PD-L1 sandwich structure. We also enhanced the SPR signal via AuNPs to further improve sensor sensitivity. The SPRi sensor is able to measure sPD-L1 within a linear range of 50 pM–10 nM and 100 fM–50 pM, and the minimum detection limit is 19 fM. The sensor is designed to be widely applicable, with better accuracy and reliability for more application scenarios. The prepared SPRi sensor shows great potential in improving the sensitivity of detecting sPD-L1. The proposed method demonstrates the excellent performance of the SPRi sensor and provides a possibility for the establishment of effective clinical assay methods in the future.Keywords:programmed death-ligand 1;surface sandwich assay;DNA aptamer/antibody bioreceptor pair;surface plasmon resonance;tumor biomarker
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高影响因子
OPTICAL MATERIALS
·
2022
·
IF 3.754
Fenglei Sun,Zhenlei Yuan,Peng Guo,Haifeng Zhou,Jiaxuan Wang,Chuan Liao,Caoyuan Niu,Guangjun Zhou,Xiaoqiang Yu
In this study, we designed a dual functional nanosensor for detecting Cr (Ⅲ) and sodium tripolyphosphate (STPP) based on fluorescence resonance energy transfer (FRET) mechanism between amine functionalized upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs), where UCNPs serve as energy donor and aggregated AuNPs as energy acceptor. UCNPs have red emission at 654 nm under 980 nm laser excitation and monodispersed AuNPs show strong absorbance around 520 nm. Monodispersed AuNPs form aggregate easily in the presence of Cr (Ⅲ), which leads to the red-shift of the absorption of AuNPs and quenches the red emission of UCNPs. In the presence of STPP, the formation of Cr (Ⅲ)-STPP complex weakens the aggregation extent of AuNPs and the red emission of UCNPs increases. Therefore, the content of Cr (Ⅲ) and STPP could be detected in wide range according to the linear variety of fluorescence intensities of UCNPs at 654 nm with the detection limits of 0.44 μM and 0.54 μM for detecting Cr (Ⅲ) and STPP, respectively. In addition, due to the high selectivity and accuracy for the detection of STPP, this nanosensor can be utilized to determine STPP in green tea drink and drinking water.
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高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2020
·
IF 3.807
Xiao Yang,Minghui Feng,Jianfei Xia,Feifei Zhang,Zonghua Wang
A novel electrochemical biosensor based on AuNPs/Ti 3 C 2 MXene three-dimensional nanocomposite for sensitive miRNA-155 detection by exonuclease III (Exo III)-aided cascade target recycling is reported. Ti 3 C 2 MXene is synthesized and used as a support to immobilize Au nanoparticles (AuNPs) due to its special layered structure and metallic properties. The three-dimensional (3D) structure of the AuNPs/Ti 3 C 2 MXene nanocomposite for the biosensor platform leverages the integrated advantages of a large specific surface area, excellent electrical conductivity and electrocatalytic properties . AuNPs are used to immobilize capture DNA (C-DNA) by Au S chemical bonds. The C-DNA was modified with methylene blue (MB) at the 3′ end, resulting in an initial DPV electrochemical signal ( I d ). In the presence of target, miRNA-155 and C-DNA formed a double-stranded structure by complementary base pairing. Subsequently, Exo III digested the 3′ end of the C-DNA in the double-stranded structure and caused the electrochemical signal to “turn off” (the final signal denoted as I h ). By Exo III-aided cascade cleavage of the target, one miRNA-155 chain causes the release of several C-DNAs under certain conditions, which significantly amplifies the peak current difference on the fabricated biosensor (Δ I = I d ﹣ I h ). The electrochemical sensor achieved a detection limit of 0.35 fM (S/N = 3) with a linear range from 1.0 fM to 10 nM. This sensor also shows desirable stability, reproducibility and specificity.
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高影响因子
TALANTA
·
2020
·
IF 5.339
Weiwei Ma,Shuangfeng Wan,Chenchen Lin,Xucong Lin,Zenghong Xie
Highly specific sample pretreatment for the sensitive detection of trace bisphenol A (BPA) in compliacted samples is critical. Herein, a new protocol towards online specific recognition and sensitive detection of BPA was proposed by using [email protected] affinity monolith coupled with LC-MS. Optimization of polymerization conditions and characterization such as the morphology, energy spectrum, mechanical stability, aptamer coverage density and specific performance of the affinity monolith were studied. Nano-gold particles (AuNPs) densely distributed on the rigid hybrid-silica substrates, and an unusually high aptamer coverage density reached 3388 pmol/μL, which was favorable to fulfill the effective identification of BPA with high selectivity and inhibit the interference of analogs including BPB and BPC. A highly sensitive recognition of BPA was obtained with the limit of detection (LOD) as low as 0.02 ng/mL. Applied to dairy milk products and serum samples, trace BPA could be sensitive detected by this strategy, while the poor response was achieved by using traditional non-specific SPE column for sample pretreatment. Satisfactory recoveries of fortified BPA were measured as 97.45 ± 2.24%–98.03 ± 4.36% (n = 3) in powdered infant formulas, 96.64 ± 3.37% ~ 99.42 ± 3.22% (n = 3) in bottled milk, 94.69 ± 2.15% ~ 100.96 ± 1.94% (n = 3) in boxed milk, and 93.71 ± 1.53% ~ 96.73 ± 2.56% (n = 3) in children serum samples, respectively. This protocol lights a new access to online specific identification of trace BPA from complex matrix with good detection sensitivity by using aptamer-affinity monolithic column.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yuanling Sun,Yanna Lin,Weiyan Sun,Rui Han,Chuannan Luo,Xueying Wang,Qin Wei
A novel, highly selective and sensitive chemiluminescence (CL) biosensor for insulin (INS) detection was proposed based on aptamer and oligonucleotide-gold nanoparticles functionalized nanosilica @ graphene oxide aerogel . Initially, nanosilica functionalized graphene oxide aerogel (SiO 2 @GOAG) was successfully prepared and the composite showed rich pore distribution, large specific surface area and good biocompatibility. Insulin aptamer (IGA3) was used as a biorecognition element and oligonucleotide functionalized gold nanoparticles (ssDNA-AuNPs) was used as CL signal amplification materials, which were functionalized on the surface of SiO 2 @GOAG. The multi-functionalized composite - ssDNA-AuNPs/IGA3/SiO 2 @ GOAG was obtained and used to construct the CL biosensor for insulin detection. When insulin is present in a sample, the insulin will bind to the IGA3, which will result in the release of ssDNA-AuNPs. The released ssDNA-AuNPs would catalyze the luminescence of luminol and H 2 O 2 . The linear range of the CL biosensor for insulin detection was 7.5 × 10 −12 to 5.0 × 10 −9 moL/L and the detection limit was 1.6 × 10 −12 moL/L (S/N = 3). The selectivity and stability of the CL biosensor were also studied and the results showed that the biosensor exhibited high selectivity and good stability due to the introduction of ssDNA-AuNPs/IGA3/SiO 2 @GOAG. The CL biosensor was finally used for recombinant human insulin detection in recombinant human insulin injection and the results were satisfactory.
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高影响因子
ANALYST
·
2014
·Vol. 140
·期 2
·551-559
·
IF 3.906
Yuna Guo,Yu Wang,Su Liu,Jinghua Yu,Hongzhi Wang,Min Cui,Jiadong Huang
A novel electrochemical immunosensor assay (EIA) for highly sensitive and specific detection of Escherichia coli O157:H7 has been developed. This immunosensor is constructed by the assembly of capture antibody on SG–PEDOT–AuNPs composites modified glass carbon electrode. In the presence of target E. coli O157:H7, horseradish peroxidase (HRP)-labeled antibody is captured on the electrode surface to form a sandwich-type system via the specific identification. As a result, E. coli O157:H7 detection is realized by outputting a redox current from electro-reduction of hydrogen peroxide reaction catalyzed by HRP. In our assay, the combination of the unique properties of sulfonated graphene (SG) and gold nanoparticles (AuNPs) can not only accelerate electron transfer on electrode interface, but also provide an excellent scaffold for the conjugation of capture antibody that significantly improves the target capture efficiency and enhances the sensitivity of the biosensor. The results reveal the calibration plot obtained for E. coli O157:H7 is approximately linear from 7.8 × 10–7.8 × 106 colony-forming unit (cfu) mL−1 with the limit of detection of 3.4 × 10 cfu mL−1. In addition, the biosensor has been successfully applied to the quantitative assay of E. coli O157:H7 in synthetic samples (spring water and milk). Hence, the developed electrochemical-based immunosensor might provide a useful and practical tool for E. coli O157:H7 determination and related food safety analysis and clinical diagnosis.
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-11
·
IF 5.3
Hao Xuanxuan,Liu Zhimin,Zheng Tongtong,Fan Yunfeng,Hu Leqian
A signal-amplified platform was designed to construct a label-free electrochemical aptasensor for lead ions (Pb 2+ ) assay. First, flower-like molybdenum disulfide-supported AuNPs (AuNPs@MoS 2 ) nanocomposites were synthesized and used as substrates for modifying the electrode. The AuNPs@MoS 2 material possessed large surface area and superior biocompatibility, which was beneficial to improve the loading amount of the complementary DNA (cDNA) and amplified the response signal. Importantly, the prepared core–shell Pt@Pd bimetallic nanoparticles (Pt@PdNPs) were used to conjugate with redox marker thionine (Thi) and aptamer (Apt) for further signal amplification; the obtained signal probes (Thi-Pt@PdNPs-Apt) were connected by the cDNA assembled on the electrode through DNA hybridization. Differential pulse voltammetry was performed to monitor the signal of Thi. After incubating of aptasensor with Pb 2+ , the specific recognition of Pb 2 + and Apt resulted in the dissociation of aptamer-cDNA complex, thereby the Thi-Pt@PdNPs-Apt separated from the electrode surface and decreased current response was obtained. The prepared electrochemical sensor exhibited linear response to Pb 2+ in the range 5.0 × 10 −4 –100 nM and a detection limit of 1.0 × 10 −4 nM was achieved. The sensor was applied to the determination of Pb 2+ in actual sample with high sensitivity and accuracy, demonstrating potential applications in heavy metal monitoring. Graphical Abstract
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高影响因子
Advanced Science
·
2025
·
IF 14.1
Xinyu Xu,Hao Wang,Xiaohan Mei,Dapeng Zeng,Zehao Yu,Shibo Liu,Ruiyan Li,Yanguo Qin
Malignant bone tumor defects are difficult to treat because of the simultaneous need for tumor treatment and bone-repair promotion. This study presents a bioactive composite scaffold (T-rGO@Au) for personalized bone defect repair and bone tumor treatment. The T-rGO@Au scaffold has a porous structure, and its mechanical properties are close to those of human cancellous bone. The T-rGO@Au scaffold can induce upregulation of osteopontin (OPN), RUNX-2, and osteocalcin (OCN) gene expression. In vivo experiments showed that the bone volume/total volume (BV/TV) ratio with the T-rGO@Au scaffold was the highest. The new bone was tightly integrated with the implant, demonstrating effective osseointegration. The T-rGO@Au scaffold locally generated high temperatures and reactive oxygen species under near-infrared excitation, and AuNPs enhanced the photothermal performance of rGO through the “antenna effect.” Furthermore, in vitro experiments showed that the tumor cell nuclei were destroyed, late-stage apoptotic cells increased, and cell morphology was severely damaged. Additionally, RNA-seq revealed that tumor cell destruction was mediated through signaling pathways, such as the MAPK pathway. In vivo antitumor experiments also demonstrated that the T-rGO@Au scaffold significantly inhibited the growth of tumor cells within 2 weeks. Thus, the T-rGO@Au scaffold provides a new treatment strategy for the development of implantable scaffolds for bone tumor defects.
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yuanqian Xiong,Xiaoyu Gao,Yu Wang,Xiangrui Deng,Chunhong Xiong,Jinsheng Zhang,Qianhui Gu,Ganhui Huang
Norfloxacin (NOR) is now extensively used for the treatment of human and animal diseases. Unfortunately, large accumulations of NOR will pose a significant threat to the human body. Thus, it is urgent to develop a rapid and sensitive detection technique for the trace determination of NOR. In this study, an electrochemical molecular imprinting sensor with great properties was fabricated for the determination of NOR in milk and environmental water. The incorporation of bovine serum proteins produced a hybrid recognition interface consisting of molecularly imprinted polymers and peptides that provided good recognition properties for norfloxacin. The Fe 3 O 4 @ZIF-8 was distributed orderly on the electrode after the embedding of the magnets inside the electrode, then the BSA was coated on the electrode with the electrodeposited AuNPs through Au-S bonding. The sensor constructed in this study achieved ultra-sensitive detection of norfloxacin with an effective area of 0.08433 cm 2 , a limit of detection (LOD) of 0.108 pmol·L −1 , and a wide linear range (1 ∼ 100, 100 ∼ 1000 and 1000 ∼ 8000 pmol·L −1 ). The prepared sensors exhibited satisfactory performance in the detection of milk and environmental water, with acceptable recoveries ranging from 96.24 % to 104.88 %, and the relative standard deviation (RSD) was no more than 4.84 %.
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-12
·
IF 5.3
Wang Yisi,Zhang Junying,Lv Xinxin,Ding Ya,Wang Yaolong,Liu Yuanhua,Wu Chunyong,Yang Gongjun
A highly sensitive and selective label-free impedimetric immunosensor based on AuNPs@pDA@NiCo 2 S 4 @MoS 2 nanocomposite modified on the surface of a screen-printed electrode (SPE) was designed for the detection of interleukin-6 (IL-6). The distribution of NiCo 2 S 4 nanoparticles on MoS 2 nanosheets was able to prevent them from agglomerating. The polydopamine (pDA) layer was coated on the surface of NiCo 2 S 4 @MoS 2 nanosheets by self-polymerization, which improved the stability and biocompatibility of the nanomaterial. The excellent reduction ability of pDA promoted the synthesis of gold nanoparticles (AuNPs), which increased the amount of antibody adsorption and the conductivity of the material. Finally, the antibody (Ab) of IL-6 was immobilized on the surface of AuNPs@pDA@NiCo 2 S 4 @MoS 2 nanocomposite. Electrochemical impedance spectroscopy (EIS) was used to detect the change of impedance before and after the immune response between Ab and IL-6 antigen (IL-6). Under the optimal experimental conditions, the relative change in impedance and the logarithmic concentration of IL-6 showed a good linear relationship in the range 1.00 to 1.00 × 10 6 pg/mL, with a low detection limit of 0.97 pg/mL. In addition, the proposed immunosensor performed with good reproducibility, stability, and specificity. It was successfully applied to the determination of IL-6 in patient’s serum samples of head and neck carcinoma with recoveries of 98.40% to 106.5%. To sum up, the proposed label-free impedimetric immunosensor was successfully constructed for IL-6 detection in real samples. Graphical abstract
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高影响因子
Small
·
2018
·Vol. 14
·期 40
·1802292
·
IF 9.598
Keying Zhang,Shuting Song,Shan Huang,Lin Yang,Qianhao Min,Xingcai Wu,Feng Lu,Jun-Jie Zhu
Intracellular microRNAs imaging based on upconversion nanoprobes has great potential in cancer diagnostics and treatments. However, the relatively low detection sensitivity limits their application. Herein, a lock-like DNA (LLD) generated by a hairpin DNA (H1) hybridizing with a bolt DNA (bDNA) sequence is designed, which is used to program upconversion nanoparticles (UCNPs, NaYF 4 @NaYF 4 :Yb, Er@NaYF 4 ) and gold nanoparticles (AuNPs). The upconversion emission is quenched through luminescence resonance energy transfer (LRET). The multiple LLD can be repeatedly opened by one copy of target microRNA under the aid of fuel hairpin DNA strands (H2) to trigger disassembly of AuNPs from the UCNP, resulting in the lighting up of UCNPs with a high detection signal gain. This strategy is verified using microRNA-21 as model. The expression level of microRNA-21 in various cells lines can be sensitively measured in vitro, meanwhile cancer cells and normal cells can be easily and accurately distinguished by intracellular microRNA-21 imaging via the nanoprobes. The detection limit is about 1000 times lower than that of the previously reported upconversion nanoprobes without signal amplification. This is the first time a nonenzymatic signal amplification method has been combined with UCNPs for imaging intracellular microRNAs, which has great potential for cancer diagnosis.
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高影响因子
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