JOURNAL OF PHYSICAL CHEMISTRY B
·
2014
·
IF 3.377
Long-Hai Wang,Sheng-Gang Ding,Jun-Jie Yan,Ye-Zi You
Encapsulation of negatively charged plasmid DNA into a small-sized nanocapsule without using any condensing agent is very challenging up to now. Here we report a versatile method for encapsulating large-sized plasmid DNAs into small-sized bioreducible nanocapsules in which shearing force and surfactant can fold large-sized plasmid DNAs into small-sized emulsion droplets containing bioreducible branched polymers. Subsequently, temperature triggers the bioreducible branched polymers to aggregate and cross-link at the water/oil interface of the emulsion nanodroplet, forming a bioreducible shell around the nanodroplet. Thus, a small-sized nanocapsule (∼110 nm) containing large-sized plasmid DNA (∼1900 nm long) forms by removal of the surfactant.
研究领域:
生物材料学
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基因治疗
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药剂学
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DOI
高影响因子
Biosensors-Basel
·
2020
·Vol. 10
·期 12
·211
·
IF 3.24
Min-Xin Mao,Rong Zheng,Chi-Fang Peng,Xin-Lin Wei
In this work, a paper device consisted of a patterned paper chip, wicking pads, and a base was fabricated. On the paper chip, DNA–gold nanoparticles (DNA–AuNPs) were deposited and Hg2+ions could be adsorbed by the DNA–AuNPs. The formed DNA–AuNP/Hg2+nanozyme could catalyze the tetramethylbenzidine (TMB)–H2O2chromogenic reaction. Due to the wicking pads, a larger volume of Hg2+sample could be applied to the paper device for Hg2+detection and therefore the color response could be enhanced. The paper device achieved a cut-off value of 50 nM by the naked eye for Hg2+under optimized conditions. Moreover, quantitative measurements could be implemented by using a desktop scanner and extracting grayscale values. A linear range of 50–2000 nM Hg2+was obtained with a detection limit of 10 nM. In addition, the paper device could be applied in the detection of environmental water samples with high recoveries ranging from 85.7% to 105.6%. The paper-device-based colorimetric detection was low-cost, simple, and demonstrated high potential in real-sample applications.Keywords:paper device;signal enhancement;mercury ion;colorimetric detection
研究领域:
分析化学
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环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Jiaona Xu,Chunying Wei
Two general and reliable fluorescence sensors were proposed in this work utilizing aptamer DNA-templated silver nanoclusters (Ag NCs). Both DNA-AgNCs could be used for label-free detecting of ATP with the limits of detection of 0.44 and 0.65 mM. One of them was further applied to monitor the activity of adenosine deaminase (ADA). In our effort to elucidate the light-up mechanism, we studied a total of six Ag NCs prepared by different DNA sequences , and found that they showed different sensitivity to ATP. Both BT3T3- and BT3T3(R)-templated Ag NCs were chose to make particular studies by UV–vis, TEM, fluorescence, and TCSPC methods. The results showed that when DNA-Ag NCs was kept for 1.5 h and presented a strong fluorescence, the addition of ATP failed to cause a large change of fluorescence intensity ; on the contrary, after Ag NCs was kept for 24 h and emitted a weak fluorescence, adding ATP was able to result in the large fluorescence enhanced of 43 and 33 times for BT3T3- and BT3T3(R)-templated Ag NCs, respectively. The possible mechanism was also suggested that ATP binding to aptamer segment of template induced the change of the DNA secondary structure , which made the aggregated Ag nanoparticles disperse into Ag NCs with an average diameter of about 2 nm that were responsible for the large fluorescence increase. Moreover, ATP could protect the fluorescence intensity of BT3T3(R)-templated Ag NCs from quenching for at least 9 h.
研究领域:
生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 28
·3584-3589
·
IF 2.378
Yanqiong Lai,Xia Teng,Yanli Zhang,Hongbin Wang,Pengfei Pang,Chun Yang,Colin J. Barrow,Wenrong Yang
In this study, we developed a simple, sensitive, low-cost and label-free method to detect rutin by using double-stranded DNA-templated copper nanoclusters (dsDNA-CuNCs) as a fluorescent probe. The dsDNA-template can facilitate the formation of highly fluorescent CuNCs with a maximum emission wavelength of 575 nm. In the dsDNA-CuNC fluorescent probe, rutin was intercalated into the structure of dsDNA via hydrogen bonding and electrostatic interactions. The fluorescence of dsDNA-CuNCs was quenched considerably due to the electron transfer from CuNCs to rutin. This decrease in the fluorescence intensity of dsDNA-CuNCs can be used to monitor the concentration of rutin from 0.6 to 400 μM with a detection limit of 0.12 μM (S/N = 3). Compared with the previous reports, this method does not require any complex DNA sequence design, fluorescent dye label and sophisticated experimental techniques. Moreover, the proposed sensing system could be applicable for the detection of rutin in pharmaceutical samples.
研究领域:
生物化学
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分析化学
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药物分析
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DOI
高影响因子
NANOTECHNOLOGY
·
2013
·Vol. 24
·期 34
·345502
·
IF 3.842
Guiying Liu,Yong Shao,Jian Peng,Wei Dai,Lingling Liu,Shujuan Xu,Fei Wu,Xiaohua Wu
Double-stranded DNAs (ds-DNAs) have been identified as efficient templates favoring the formation of fluorescent copper nanoparticles (Cu NPs). Herein, we have tried to synthesize fluorescent Cu NPs using single-stranded DNAs (ss-DNAs) as templates and to identify the critical DNA sequences. By comparing the results using homopolymer DNAs, hairpin DNAs, and pristine ss-DNAs as templates, we found that DNA thymine base plays a dominant role in producing red-emissive fluorescent Cu NPs on ss-DNA templates. The thymine-dependent growth of the fluorescent Cu NPs is confirmed by Hg2+ mediated T–T base pair in comparison with the other non-specific metal ions, which could be developed into a practical sensor for turn-on fluorescence detection of Hg2+ with a high selectivity. The mechanism is briefly discussed according the DNA sequence-dependent formation of fluorescent Cu NPs. This work demonstrates the sequence role in producing fluorescent Cu NPs that could serve as promising fluorescent nanoprobes in biosensing and DNA-hosted Cu nanomaterials.
研究领域:
纳米技术
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生物化学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2025
·
IF 5.5
Mengyao Cao,Xiewei Xiong,Li Li,Hao Pei
Comprehensive Summary Monitoring and analyzing expression levels of multiple biomarkers in biological samples can improve disease risk prediction and guide precision medicine but suffers from high cost and being time-consuming. Here, we construct a fast molecular classifier based on freeze-thaw cycling that implements an in silico support vector machine (SVM) classifier model at the molecular level with a panel of disease-related biomarkers expression patterns for rapid disease diagnosis. The molecular classifier employs DNA reaction networks as the computing module and repeated dehydration and concentration process as the driving force to implement a set of simplified mathematical operations (such as multiplication, summation and subtraction) for efficient classification of complex input patterns. We demonstrate that the fast DNA-based molecular classifier enables precise cancer diagnosis within a short turnaround time in synthetic samples compared to those of free diffusion classifiers. We envision that this all-in-one molecular classifier will create more opportunities for inexpensive, accurate, and rapid disease diagnosis, prognosis and therapy, particularly in emergency departments or the point of care.
研究领域:
生物信息学
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分子生物学
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医学诊断
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Jian Chu,Chun Chen,Xijian Li,Libing Yu,Wenjing Li,Mengxi Cheng,Wei Tang,Zhonghua Xiong
It is of great significance to develop facile and economical strategies for on-site detection and treatment of toxic metal ions . Stimulus-responsive DNA hydrogel materials have been increasingly used for convenient detection of metal ions due to their advantages such as simplicity, portability, and ease of storage. However, these methods still require encapsulation of signal tags by labeling or embedding. In this paper, a one-step preparation of Pb 2+ -responsive pure DNA hydrogel material was designed to realize a new label-free strategy for Pb 2+ biosensing. The Pb 2+ -dependent DNAzyme strand and substrate strand were introduced to fabricate the DNA hydrogel. The presence of Pb 2+ in the sample activates the enzyme strand in the hydrogel skeleton and triggers the cleavage of the substrate, thereby destroy the hydrogel structure. DNA fragments released by the collapsed hydrogel were readily measured as signal output for quantifying Pb 2+ concentrations with a minimum detection limit of 7.7 nM. We successfully eliminated the need for embedding or labeling of signal molecules by using the DNA molecules that construct hydrogels as the signal output. And the newly developed method for label-free detection of Pb 2+ based on pure DNA hydrogel is simple, easy readout, and cost-effective. By adjusting the DNAzyme and substrate sequences, label-free analysis of other metal ions can also be achieved. We expect that our strategy can be applied to the field detection of toxic metal ions.
研究领域:
环境科学
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材料科学
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生物传感
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
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2022
·Vol. 12
·期 3
·162
·
IF 5.743
Yitao Liang,Bin Zhang,Zexin Xue,Xuesong Ye,Bo Liang
Aberrations of genomic DNA methylation have been confirmed to be involved in the evolution of human cancer and have thus gained the potential to be depicted as biomarkers for cancer diagnostics and prognostic predictions, which implicates an urgent need for detection of total genomic DNA methylation. In this work, we suggested an assay for the quantification of global DNA methylation, utilizing methylation specific antibody (5mC) modified magnetic beads (MBs) for immunorecognition and affinity enrichment. Subsequently, the captured DNA on the surface of MBs interacted with the glucose oxidase-conjugated DNA antibody whose catalytic reaction product was engaged in electrochemical detection of the overall level of DNA methylation on a PB-doped screen-printed electrode. With 15 pg of input DNA, which, to our best knowledge, is the lowest required amount of DNA without sodium bisulfite treatment or amplification, this test strategy was able to perceive as low as 5% methylation level within 70 min including the preparation of anti-5mC-MBs. We believe this detection technique offers a promising option to detect global DNA methylation in both academic and clinical scenarios.Keywords:DNA methylation;magnetic beads;screen printed electrode;electrochemical
研究领域:
生物化学
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生物技术
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癌症研究
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分析化学
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产品关联
DOI
PubMed
高影响因子
INORGANICA CHIMICA ACTA
·
2015
·
IF 2.046
Liansheng Cui,Caifeng Bi,Yuhua Fan,Xin Li,Xiangmin Meng,Nan Zhang,Zhongyu Zhang
We have synthesized three novel organobismuth(V) complexes Ph 3 Bi(OOCC 6 H 3 F 2 ) 2 1 , Ph 3 Bi(OOCC 6 H 4 CF 3 ) 2 2 and Ph 3 Bi(OOCC 4 H 3 S) 2 3 , and their structures were characterized by IR spectroscopy, elemental analysis, 1 H NMR and single crystal X-ray diffraction. X-ray crystallography showed that all of the bismuth atoms adopt distorted trigonal–bipyramidal geometries. The complex molecules lead to one-dimensional chain structure and two-dimensional network structure through C–H…X (X = O, F, C) interactions. The interaction of the complex 1 with calf-thymus DNA (CT-DNA) was investigated by UV absorption spectroscopy, fluorescence emission spectroscopy and viscosity. All results revealed that the complex 1 binds to DNA via a intercalative mode. Furthermore the proliferation inhibitory activities of the three complexes on MDA-MB-231 breast cancer cells were investigated. The results indicated that all of the three complexes have superior inhibition of cellular proliferation.
研究领域:
化学
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分子生物学
·
药理学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jingyi Cai,Junping Ma,Yuan Dang,Shuangli Chen,Jieli Tang,Sha Yu,Yuanzhen Zhou
Antibiotics residue pollution in food and environmental matrices has posed a serious threat to global public health. It is an urgent demand in developing highly sensitive methods for the detection of low-abundance antibiotics in real samples. Herein, we established a magnetic DNA walker-engineered electrochemical sensor by taking advantages of the distinct recognition capability of aptamer and the excellent signal amplification performance of magnetic DNA walker for the accurate detection of kanamycin (Kana). The aptamer can specifically recognize Kana, while the magnetic DNA walker can not only amplify the target signal through the cascade displacement reaction, but also effectively reduce background interference via the magnetic separation, thus ensuring the sensitivity and accuracy of Kana detection in complex samples. The proposed electrochemical sensing method possesses a good linear relationship in the range of 10 fM −1 μM, and the limit of detection is as low as 0.02 fM. Moreover, the excellent selectivity allows this sensor has the ability to specifically identify the Kana from other antibiotics and even in the milk and tap water.
研究领域:
生物传感
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环境科学
·
分析化学
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产品关联
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-5
·
IF 4.259
Shi Chao,Wang Yifan,Zhang Menghua,Ma Cuiping
Nucleic acids have been shown to be versatile molecules and engineered to produce various nanostructures. However, the poor rate of these uncatalyzed nucleic acid reactions has restricted the development and applications. Herein, we reported a novel finding that DNA self-assembly could be nonenzymatically catalyzed by artificial agents with an increasing dissociation rate constant K2. The catalytic role of several artificial agents in DNA self-assembly was verified by real-time fluorescent detection or agarose gel electrophoresis. We found that 20% PEG 200 could significantly catalyze DNA self-assembly and increase the reaction efficiency, such as linear hybridization chain reaction (HCR) and exponential hairpin assembly (EHA). Therefore, we foresee that a fast and efficient DNA self-assembly in structural DNA nanotechnology will be desirable.
研究领域:
结构DNA纳米技术
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生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Xiuli Wang,Qilin Guo,Jia Guo,Changchun Wang
Rapid extraction and screening of high purity DNA fragments is an indispensable technology in advanced molecular biology. In this article, mesoporous magnetic composite microspheres (MSP@mTiO2) with tunable pore sizes are successfully fabricated for high purity DNA extraction and fragment screening. Due to the strong complexation ability of Ti4+ with DNA phosphate groups and the high specific surface area of mesoporous microspheres, the MSP@mTiO2 microspheres possess excellent adsorption performance, the saturated loading capacity of MSP@mTiO2 with specific surface area of 122 m2/g is as high as 575 g/mg for salmon sperm specimen. ITC experiment demonstrates that the DNA adsorption on MSP@mTiO2 microspheres is mainly driven by entropy, which gives us more potential ways to regulate the balance of adsorption and desorption. Meanwhile, the mesoporous MSP@mTiO2 microspheres exhibit much higher extraction efficiency compared with non-porous MSP@TiO2 for whole genome DNA from Arabidopsis thaliana plants. Interestingly, the DNA frangements with different lengths can be screened by simply regulating the pore size of MSP@mTiO2 or concentration of Na3PO4 in eluent. Small pore size and low phosphate concentration are advantageous for the extraction of short-stranded DNA fragments, the DNA fragments (≤1000 bp) can be efficiently extracted when the mesopore size of MSP@mTiO2 is lower than 7.6 nm. The extraction results from the mesoporous composite microspheres provide new promising insights on the DNA purification and screening from complex biological samples.
研究领域:
分子生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）