Food Bioscience
·
2024
·
IF 4.8
Zixuan Hu,Chunfeng Liu,Chengtuo Niu,Jinjing Wang,Feiyun Zheng,Qi Li
Brewer's spent grain (BSG) and brewer's spent yeast (BSY) are two major brewing by-products, which are low-cost raw materials with high yields and nutritional value. In this study, the antioxidant peptides from brewing by-products were separated, purified and identified by UF, GFC or HIC, semi-preparative RP-HPLC, and nano-HPLC-MS/MS. Thereafter, in silico analysis was applied to predict the bioactivity, toxicity, and bioavailability of the identified peptides, and a total of 23 potential novel peptides with antioxidant activity and bioavailability were excavated. Furthermore, molecular docking results revealed the potential antioxidant peptides had stable binding ability to Keap1, and Tyr334, Arg380, Arg415, Arg483, and Tyr525 residues contributed enormously to docking, all of which were located in the binding sites of Keap1-Nrf2 interaction. Peptides with the lowest binding energy (i.e. AGRLPW, DRLW, KPW, PLWWRHPR, RVWNPFR, and ANLPWLGK) were further synthesized and validated in vitro and at the cellular level to be bioactive. Western blot results showed that KPW, RVWNPFR, and ANLPWLGK could protect HaCaT cells from H 2 O 2 -induced oxidative damage by activating Keap1-Nrf2 pathway. These findings provided insights into the high value-added utilization of brewing by-products.
研究领域:
食品科学
·
生物化学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xueyao Wang,Xuyang Hu,Zhengjun Qu,Ting Sun,Lihui Huang,Shimin Xu
Molybdenum disulfide (MoS2)-based materials for piezocatalysis are unsatisfactory due to their low actual piezoelectric coefficient and poor electrical conductivity. Herein, 1T/3R phase MoS2 grown in situ on multiwalled carbon nanotubes (MWCNTs) was proposed. MoS2@MWCNTs exhibited the interwoven morphology of thin nanoflowers and tubes, and the piezoelectric response of MoS2@MWCNTs was 4.07 times higher than that of MoS2 via piezoresponse force microscopy (PFM) characterization. MoS2@MWCNTs exhibited superior activity with a 91% degradation rate of norfloxacin (NOR) after actually working 24 min (as for rhodamine B, reached 100% within 18 min) by pulse-mode ultrasonic vibration-triggered piezocatalysis. It was found that piezocatalysis for removing pollutants was attributed to the synergistic effect of free radicals (•OH and O2•–) and nonfree radical (1O2, key role) pathways, together with the innergenerated-H2O2 promoting the degradation rate. 1O2 can be generated by electron transfer and energy transfer pathways. The presence of oxygen vacancies (OVs) induced the transformation of O2 to 1O2 by triplet energy transfer. The fast charge transfer in MoS2@MWCNTs heterostructure and the coexistence of sulfur vacancies and OVs enhanced charge carrier separation resulting in a prominent piezoelectric effect. This work opens up new avenues for the development of efficient piezocatalysts that can be utilized for environmental purification.
研究领域:
材料科学
·
环境科学
·
催化
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Ke Yang,Li Wang,Jinyi Chen,Zefeng Wang,Junqin Li,Xi Chen,Shuting Fu,Luo Hai,Le Deng,Dinggeng He
High H 2 O 2 levels are widely present at the infection sites or in the biofilm microenvironment. Herein, hemin with peroxidase-like catalytic activity and its substrate, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), are simultaneously introduced into a liposomal nanoparticle containing thermosensitive 2,2′-azobis[2-(2-imidazolin-2-yl) propane] dihydrochloride (AIBI)-loaded bovine serum albumin (BAG), rationally constructing an H 2 O 2 -activatable liposomal nanobomb (Lipo@BHA) for combating biofilm-associated bacterial infections with high performance. In the presence of H 2 O 2 , hemin can catalyze the conversion of ABTS into its oxidized form (ABTS· + ) with strong near-infrared (NIR) absorption, which produces photonic hyperpyrexia to cause the decomposition of AIBI into oxygen-independent alkyl radicals (·R) and nitrogen (N 2 ) microbubbles. The former not only directly damage bacterial cells but also significantly accelerates the oxidization of ABTS to ABTS· + for augmenting photothermal-triggered generation of ·R. Interestingly, the released N 2 can induce transient cavitation to rupture lysosomal nanoparticle and improve the biofilm permeability, thereby enhancing the antibiofilm effect of Lipo@BHA. The proposed Lipo@BHA exhibits satisfactory multi-mode combination antibacterial properties. Through endogenous H 2 O 2 -activated cascade reaction, Lipo@BHA achieves remarkable hypoxia-irrelevant ·R therapy of biofilm-associated wound infections with low cytotoxicity and good in vivo biosafety. Therefore, this work presents a versatile H 2 O 2 -activatable cascade ·R generation strategy for biofilm-specific therapeutic applications.
研究领域:
纳米技术
·
生物医学工程
·
药学
·
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2022
·
IF 10.53
Xuemei Bao,Qianqian Liu,Jiahui Yang,Furong Wang,Fengjiao Yu,Jian Yu,Yang Yang
Fouling control is of great significance for polymeric membrane-based wastewater purification and reclamation. Herein, we designed a reactive polysulfone (PSF) ultrafiltration (UF) membrane integrating resorcinol–formaldehyde (RF) resin nanospheres and β -FeOOH nanoparticles ( [email protected] /PSF) with unique self-cleaning performance. The [email protected] /PSF membrane with an optimal RF resin and β -FeOOH loading showed a reasonable water flux (376.3 L m −2 h −1 bar −1 ) and high rejection (97.5%) to bovine serum albumin. Besides, the membrane could capture trace amount of cationic dyes in the feed solution via electrostatic attraction. After organic fouling, the permeation flux of the membrane was effectively recovered under visible-light irradiation at neutral pH. We confirmed that the low-bandgap RF resin nanospheres blended in the PSF matrix produced H 2 O 2 in-situ upon absorption of visible light. Then the adjacent β -FeOOH nanoparticles catalyzed the conversion of the self-supplied H 2 O 2 to •OH radicals. The [email protected] /PSF membrane provided a unique platform to perform cascade reaction for production of •OH radicals concentrated in restricted space, which effectively degraded the adsorbed organic foulants. The proposed self-cleaning mechanism that can work under sunlight and ambient condition is expected to simplify and economize the cleaning operations of polymeric UF composite membranes in complex wastewater purification.
研究领域:
环境工程
·
水处理
·
光催化
·
膜科学
·
废水回用
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2019
·Vol. 9
·期 1
·1-13
·
IF 4.011
Hou Bolin,Wang Gang,Gao Quan,Wei Yanjie,Zhang Caining,Wang Yange,Huo Yuqing,Yang Huaiyi,Jiang Xuejun,Xi Zhijun
Sunitinib (ST), a multitargeted receptor tyrosine kinase inhibitor, has been demonstrated to be effective for the treatment of renal carcinoma. It has been reported that ST is involved in the mediation of autophagy; however, its regulatory role in the autophagic process remains controversial. Furthermore, the mechanism by which activated AMP-activated protein kinase (AMPK) negatively regulates autophagy remains nearly unexplored. In the present study, we revealed that ST inhibited AMPK activity and regulated autophagy in a cell type- and dose-dependent manner. In a number of cell lines, ST was demonstrated to inhibit H 2 O 2 -induced autophagy and the phosphorylation of acetyl-CoA carboxylase (ACC), whereas alone it could block the autophagic flux concurrent with increased expression of p62. An immunoprecipitation assay revealed that LC3 directly interacted with p62, whereas ST increased punctate LC3 staining, which was well colocalized with p62. Taken together, we reveal a previously unnoticed pathway for ST to regulate the autophagic process, and p62, although often utilized as a substrate in autophagy, plays a critical role in regulating the inhibition of ST in both basal and induced autophagy.
研究领域:
肿瘤学
·
细胞生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2020
·
IF 10.317
Guangzhen Yang,Mengni Fan,Jingwu Zhu,Chen Ling,Lihuang Wu,Xin Zhang,Ming Zhang,Jiayi Li,Qingqiang Yao,Zhongwei Gu,Xiaojun Cai
Specifically inhibiting the proliferation of activated macrophages and clearing the high levels of reactive oxygen species (ROS) secreted by macrophages is crucial for osteoarthritis (OA) treatment. Moreover, if the clearance of these high levels of ROS can be simultaneously used to induce oxidation-responsive release of anti-inflammatory drugs, the therapeutic effect of OA may be further improved. Here, a multifunctional anti-inflammatory drug (CPHs) based on a peptide dendrimer nanogel was constructed by physically encapsulating CORM-401 and wrapping its surface with folic acid (FA)-modified hyaluronic acid (HA). CPHs is capable of efficiently entering activated macrophages via FA- and HA-mediated specific targeting effects and then rapidly release large amounts of CO by massive consumption of H 2 O 2 . The generated CO effectively suppresses the secretion of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α by inhibiting cell proliferation ; inducing the activation of heme oxygenase (HO-1), and downregulating the expression of p38 MAPK , NF-kB (p50/p65) and TLR-2. In vivo experiments further confirmed that CPHs can massively deplete ROS in OA joints and effectively suppress the degradation of articular cartilage and their extracellular matrix. More importantly, CPHs is non-toxic to normal macrophages, and the high levels of CO generated in the joints do not result in notable changes in the HbCO levels in blood. Together, these results show that CPHs is an effective and safe anti-inflammatory drug and has essential application prospects in OA treatment.
研究领域:
药理学
·
炎症
·
纳米技术
·
骨科
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHEMISTRY COMMUNICATIONS
·
2012
·
IF 4.859
Xiangheng Niu,Chen Chen,Hongli Zhao,Jie Tang,Yuxiu Li,Minbo Lan
A new screen-printed carbon electrode with porous architectures prepared using an extremely facile and low-cost approach is introduced. The preparation mainly involved a printing procedure of a graphite-based layer doped with CaCO 3 powders and a subsequent dissolution of these powders. The resulting porous screen-printed carbon electrode (P-SPCE) can offer large surface, broad potential window, low background current and high electrochemical reactivity. Moreover, the proposed P-SPCE provides enhanced performance for enzyme-free H 2 O 2 sensing compared to conventional glassy carbon electrodes and screen-printed carbon electrodes. These attractive properties of the P-SPCE are expected to be of wider applications.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Yamei Gao,Shuangshuang Cheng,Qiong Chen,Ling Fu,Paiyu Lin,Beibei Yang,Pingping Wang,Haiyan Cheng,Shenggang Ding,Shaohu Huo
In cases of pneumococcal pneumonia, there is significant exposure of the human airway epithelium to hydrogen peroxide (H2O2), a toxin secreted by pathogens. This exposure can trigger oxidative stress and enhance vascular permeability, which may result in systemic bacteremia or sepsis. Drug delivery systems (DDSs) that respond to H2O2 and utilize amphiphilic polymers have attracted considerable interest. In this study, we present an amphiphilic diblock copolymer (DPB) formed from poly(ethylene glycol) (PEG) and H2O2-sensitive phenylboronic ester (PBBA), capable of self-assembling into micelles in an aqueous environment. In this work, we demonstrated the efficacy of the spherical micelle DPB@(Amp+Cat), which encapsulates the antibiotic ampicillin (Amp) and the antioxidant catalase (Cat), for the treatment of H2O2-secreting bacterial pneumonia. Through triggering antibiotic release in response to H2O2 secreted by Streptococcus pneumoniae, DPB@(Amp + Cat) eliminated over 98% of the invading bacteria, leading to a survival rate exceeding 90% in mice with severe pneumonia. Furthermore, released catalase rapidly scavenges pneumococcal H2O2, resulting in reduced endothelial DNA damage and preventing the systemic invasion of bacteria. This work presents a synergistic strategy for both antipathogen treatment and pulmonary tissue protection in pneumococcal disease.
研究领域:
药学
·
医学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Nutrition
·
2023
·
IF 5
Lu Zhang,Chun-yan Peng,Pei-xin Wang,Linju Xu,Jia-hui Liu,Xing Xie,Ling Lu,Zong-cai Tu
Radix Paeonia Alba (RPA) is often used as food and medicine. This study aimed to enrich and identify the antioxidant and hypoglycemic bioactive compounds from RPA. The results indicated that the ethyl acetate fraction (EAF) showed the highest total phenolic content, DPPH, ABTS + scavenging ability, and α-glucosidase inhibition ability (IC 50 = 7.27 μg/ml). The EAF could alleviate H 2 O 2 -induced oxidative stress in HepG2 cells by decreasing the MDA and ROS levels, improving cell apoptosis, increasing the enzyme activity of GPX-Px, CAT, SOD, Na + /K + -ATP, and Ca 2+ /Mg 2+ -ATP, and stimulating T-AOC expression, which also enhanced the glucose uptake of insulin-resistant HepG2 cells. In addition, the EAF significantly reduced the fasting blood glucose level and improved glucose tolerance in diabetic mice. An HPLC-QTOF-MS/MS analysis displayed that procyanidin, digallic acid isomer, methyl gallate, tetragalloylglucose isomer, dimethyl gallic acid, and paeoniflorin were the major compounds in the EAF. These findings are meaningful for the application of the EAF in the medicinal or food industry to prevent and treat oxidative stress and diabetes mellitus.
研究领域:
药理学
·
生物化学
·
医学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 3
·1-8
·
IF 5.705
Zong Chenghua,Li Bo,Wang Jing,Liu Xiaojun,Zhao Wenfeng,Zhang Qingquan,Nie Xinming,Yu Yang
Isotropic silver nanoparticles (iAg NPs) can be easily prepared at low costs, have a low electrochemical potential and high extinction coefficient. An effective colorimetric assay for H 2 O 2 is reported here based on the finding that H 2 O 2 can induce the shape transformation of citrate-capped iAg NPs with the help of citrate. The substantial shape variation affords an apparent surface plasmon resonance (SPR) shift, accompanied by a vivid color change from light yellow to mauve. The color change can be observed visually if the concentration of H 2 O 2 is 2 μM or higher. A good linear relationship was obtained over the concentration range of 0.2–32 μM with a limit of detection of 90 nM. By making use of glucose oxidase, the method is further extended to glucose detection. Glucose at a concentration as low as 10 μM can be well determined with bare eyes. Benefitting from the high selectivity, the detection of glucose in human serum is realized, and the results are in good agreement with those provided by a clinical analyzer.
Schematic presentation of a colorimetric assay for H 2 O 2 and glucose based on citrate-promoted H 2 O 2 -mediated shape transformation of the isotropic silver nanoparticles (Ag NPs). The shape variation of isotropic Ag NPs induces a color change from light yellow to mauve.
研究领域:
分析化学
·
生物医学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
Advanced Biosystems
·
2020
·Vol. 4
·期 3
·1900261
·
IF 0
Xiaohui Yin,Yuanqiang Sun,Xin Geng,Jianjun Li,Ran Yang,Ke Zhang,Lingbo Qu,Zhaohui Li
Mitochondria are the powerhouse of the cell, which maintain the metabolism of living cells. Mitochondrial membrane potential (MMP) is the key parameter of mitochondria and represents cellular activity. The programmable MMP transformations, i.e., normal, decrease, and vanishing, are indicative mitochondria healthy/damaged/dead states. Therefore, methods for monitoring MMP are of great importance. In this work, carbon dots responsive to mitochondrial membrane potential (MMP-CDs) are prepared by a simple microwave method. The intracellular distribution of the MMP-CDs is dependent on the mitochondrial membrane potential. Three different spatiotemporally organelle distributions (mitochondria/lysosome/nucleus) of the MMP-CDs indicate three distinct MMP levels (normal/decrease/vanishing), which represent three different cell states (healthy/damaged/dead). The normal cells with high mitochondrial membrane potential causes the MMP-CDs to accumulate in the mitochondria due to the Nernstian effect. The MMP-CDs are released from the mitochondria and transported to the lysosome when cells are in apoptosis with the decreased mitochondrial membrane potential. In dead cells with a vanished mitochondrial membrane potential, the MMP-CDs are collocated with the nucleus due to the affinity with DNA. Thus, various cellular states can be visualized through the different localizations of the MMP-CDs. These MMP-CDs are successfully employed in the detection of autophagy and H 2 O 2 -induced mitochondria and mitochondrial membrane potential changes.
研究领域:
生物化学
·
细胞生物学
✓ 已验证
DOI
Nature Communications
·
2026
·
IF 18.1
Chen Haojie,Gao Rui-Ting,Wang Lei,Wu Limin
Photoelectrochemical bipolar systems enable bias-free H 2 O 2 synthesis by integrating light harvesting, water oxidation and oxygen reduction. However, poor selectivity due to competing 4e - pathways, slow charge transfer, and inferior stability severely limit their practical application. Herein, we present a bias-free photoelectrochemical bipolar system for H 2 O 2 production, which integrates a Zn-TiO 2 /BVO/WO 3 photoanode for the 2e - water oxidation reaction and a Ru:CuO/Nb-CBO photocathode for the 2e - oxygen reduction reaction. The photoanode suppresses *OOH intermediates to favor H 2 O 2 formation, achieving a Faradaic efficiency (FE) of 90.2% at 0.6 V RHE . The photocathode stabilizes *OOH intermediates and delivers an FE of 91.9% under the same conditions while maintaining performance for over 50 h. Practical testing under natural sunlight with 36 cm 2 electrodes achieves an FE of 163.37% and a yield of 11.44 mmol L −1 . In situ generated H 2 O 2 achieves over 99% bacterial inactivation within 60 min. This work provides an adaptive platform for solar-driven H 2 O 2 synthesis and energy conversion.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）