PHYTOMEDICINE
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2024
·
IF 6.7
Dihao Xie,Simin Zhong,Meixue Luo,Jiahao Xu,Ruoyan Zheng,Jiading Luo,Yiting Wang,Yongxing Guo,Lianxia Guo,Baojian Wu,Danyi Lu
Background Aristolochic acid I (AAI), an emerging biogenic contaminant widely present in Aristolochic plants, has been implicated in the progression of tubulointerstitial disease, known as aristolochic acid nephropathy (AAN). The circadian clock, a vital regulator of organ homeostasis, is susceptible to external chemical cues, including toxins. However, the reciprocal interactions between AAI and the circadian clock remain unexplored. Methods We initially assessed sex- and time-dependent nephropathy and behavioral responses in C57BL/6J mice exposed to AAI. Subsequently, we evaluated changes in the expression of circadian clock genes following treatment with AAI or its bioactive metabolite, aristolactam I, using real-time quantitative PCR and immunoblotting in renal tissues and cells. Additionally, real-time reporter assays were conducted on kidney explants from PER2::Luc knock-in reporter mice and Per2-dLuc / Bmal1-dLuc reporter cell lines. To further elucidate the regulatory role of circadian clocks in AAI-induced nephropathy, mice with global or kidney-specific knockout of Bmal1 , as well as mice subjected to experimental jetlag, were utilized. Results Our findings revealed a sex-dependent nephrotoxicity of AAI, with males exhibiting greater vulnerability. AAI-induced nephropathy was accompanied by impaired spatial cognitive function, disruptions in free-running locomotor activity, altered renal expression of multiple core clock genes, and disturbances in the circadian rhythm of renal PER2::Luc activity. Notably, kidney-specific ablation of the core clock gene Bmal1 significantly exacerbated renal injury and inflammation, whereas disruptions to the central clock, either genetically (through conventional knockout of Bmal1 ) or environmentally (mimicking jetlag), had minimal effects on AAI nephrotoxicity. Furthermore, both AAI and its bioactive metabolite aristolactam I demonstrated the ability to disrupt circadian clocks in human osteosarcoma cells (U2OS) and mouse renal tubular epithelial cells (mRTEC). Conclusion Collectively, these findings highlight the detrimental impact of aristolochic acids on local renal circadian clocks, ultimately exacerbating kidney damage. This study provides novel insights into the molecular mechanisms underlying AAI nephrotoxicity, potentially opening avenues for therapeutic interventions aimed at modulating the renal circadian clock to treat AAN.
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Nature Communications
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2024
·Vol. 15
·期 1
·1-13
·
IF 14.7
Wu Shuyi,Luo Shulu,Cen Zongheng,Li Qianqian,Li Luwei,Li Weiran,Huang Zhike,He Wenyi,Liang Guobin,Wu Dingcai,Zhou Minghong,Li Yan
The sophisticated hierarchical structure that precisely combines contradictory mechanical and biological characteristics is ideal for biomaterials, but it is challenging to achieve. Herein, we engineer a spatiotemporally hierarchical guided bone regeneration (GBR) membrane by rational bilayer integration of densely porous N-halamine functionalized bacterial cellulose nanonetwork facing the gingiva and loosely porous chitosan-hydroxyapatite composite micronetwork facing the alveolar bone. Our GBR membrane asymmetrically combine stiffness and flexibility, ingrowth barrier and ingrowth guiding, as well as anti-bacteria and cell-activation. The dense layer has a mechanically matched space maintenance capacity toward gingiva, continuously blocks fibroblasts, and prevents bacterial invasion with multiple mechanisms including release-killing, contact-killing, anti-adhesion, and nanopore-blocking; the loose layer is ultra-soft to conformally cover bone surfaces and defect cavity edges, enables ingrowth of osteogenesis-associated cells, and creates a favorable osteogenic microenvironment. As a result, our all-in-one porous membrane possesses full protective abilities in GBR.
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Plants-Basel
·
2025
·Vol. 14
·期 22
·3535
·
IF 4.1
Xiuxiu Shen,Shengwen Chen,Mengting Zeng,Benyin Zhang
Biebersteinia heterostemon is a traditional Tibetan medicine known for its antioxidant, hypoglycemic, and anti-atherosclerotic properties. However, its therapeutic effects and mechanisms in the treatment of hyperlipidemia remain unclear. In this study, the ethyl acetate extract of B. heterostemon (BHEE) was first identified as the most effective lipid-lowering fraction through its inhibitory activity on pancreatic lipase and cholesterol esterase. Chemical characterization of BHEE by UHPLC-MS/MS revealed 108 compounds. Network pharmacology and molecular docking analyses were then employed to predict key active components and signaling pathways involved in BHEE’s lipid-lowering effects. A total of 50 active components and 623 targets were selected from the PubChem, SwissADME, and Swiss Target Prediction databases. These targets were intersected with 1606 hyperlipidemia-related targets from GeneCards, OMIM, and DrugBank, resulting in 144 common targets. The “drug-active component-intersecting target-pathway-HLP” and protein–protein interaction (PPI) networks suggested key active components such as 6-methoxytricin, vulgarin, flazin, ganhuangenin, and eupatorin, and core targets including TNF, IL6, AKT1, PPARG, and EGFR. GO and KEGG pathway enrichment analysis highlighted potential signaling pathways, such as AGE-RAGE, PPAR, insulin resistance, TNF, and lipid and atherosclerosis pathways. Molecular docking further predicted the strong binding affinity between key active components and core targets. At the cellular level, BHEE dose-dependently reduced lipid accumulation in FFA-induced HepG2 cells and improved oxidative stress (CAT, GSH, SOD, MDA) and inflammation (TNF-α, IL-6) markers. In conclusion, BHEE may exert its anti-hyperlipidemic effects through modulation of key targets like TNF, IL6, AKT1, PPARG, and EGFR. These findings suggest a multi-target mechanism, though further experimental validation is necessary to confirm these effects. This study provides valuable insights into the potential application of B. heterostemon as a natural therapeutic agent for hyperlipidemia.
研究领域:
生药学
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民族药理学
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分子生物学
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网络药理学
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天然产物研究
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心血管医学
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代谢性疾病
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生物信息学
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APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Bo Xue,Le Du,Junsu Jin,Hong Meng,Jianguo Mi
The development of a green, efficient, and reusable photocatalyst is of great importance for degradation of organic pollutants in water. Herein, MIL-88A@PA, a composite consisting of macroporous polyacrylate (PA) and metal organic frameworks (MOFs), was prepared by suspension polymerization, amine functionalization, and in-situ growth of MOFs. The nanosized MIL-88A of only 50–100 nm in diameter was reduced by approximately 90% owing to the control of heterogeneous nucleation and growth. More catalytic active sites were exposed and the photocatalytic activity of MIL-88A was significantly improved. The specific surface area of MIL-88A was increased from 132.79 to 219.78 m 2 /g owing to the decreased crystal size, and the corresponding degradation rate constant of Rhodamine B (RhB) was 9.4 times than that of pure MIL-88A. Under visible light irradiation, RhB could be completely degraded in 60 min using MIL-88A@PA with a loading amount of 45%. The degradation rate was still 97% after 5 cycles. The high photocatalytic activity of MIL-88A@PA can be attributed to the effective separation of photogenerated carriers, lower transfer resistance, and more active sites. The composite MIL-88A@PA material exhibits high stability and photocatalytic activity, which offers the potential in environmental cleaning.
研究领域:
环境科学
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材料科学
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化学工程
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光化学
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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
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2021
·Vol. 25
·期 15
·7472-7484
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IF 5.31
Pengfei Zhu,Zhengchao Wang,Zhenxing Sun,Bokai Liao,Yu Cai
Osteoarthritis (OA) is a common joint disease that mainly affects the diarthrodial joints. Treatments for OA include non-pharmacological interventions, topical and oral therapies, intra-articular therapies and joint surgery. However, all the treatments mentioned above mainly aim to control the symptoms instead of improving or reversing the joint condition. In this research, we observed the effect of recombinant platelet-derived growth factor (PDGF)-BB on OA in a monosodium iodoacetate (MIA)–induced rat model and revealed the possible mechanisms. In vitro, the level of inflammation in the chondrocytes was gradually alleviated, and the apoptosis rate was gradually decreased by PDGF-BB at increasing concentrations. The levels of p-p38, Bax and caspase-3 decreased, and the level of p-Erk increased with increasing PDGF-BB concentration. In vivo, PDGF-BB could significantly reverse chondrocyte and matrix loss. Furthermore, high concentrations of PDGF-BB could alleviate cartilage hyperplasia to remodel the tissue. The level of collagen II was up-regulated, and the levels of collagen X and apoptosis were down-regulated by increasing concentrations of PDGF-BB. In conclusion, recombinant PDGF-BB alleviated OA by down-regulating caspase-3-dependent apoptosis. The effects of PDGF-BB on OA mainly include inhibiting chondrocyte loss, reducing cartilage hyperplasia and osteophyte formation, and regulating collagen anabolism.
研究领域:
骨科
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分子生物学
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细胞生物学
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药理学
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2026
·
IF 3.9
Xuechun Li,Ze Zhang,Yang Xu,Rongxin Xia,Yuxiang Mao,Jun Cheng
For improving the activity of low-cost non-noble metal catalysts for the oxygen evolution reaction (OER) in acidic water electrolysis, a strategy was proposed to construct atomic Mn–O–Co asymmetric units within Co3O4 to form a directional built-in electric field. Mn-doped Co3O4 catalysts (MnxCo3–xO4) were synthesized via a hydrothermal-calcination method. The computational results indicated that Mn selectively occupied the octahedral Co site, forming a directional electron transfer pathway from Mn to Co, which effectively modulated the electronic structure of both Co and O. In situ Raman spectroscopy proved that the Mn–O–Co structure accelerated the efficient conversion of oxygen-containing intermediates. DFT calculations indicated that the Mn–O–Co directional built-in electric field facilitated H2O polarization via enhancing hydrogen bonding and, by optimizing the *OOH adsorption structure, lowered the OER rate-determining step barrier by 0.05 eV. The asymmetric Mn–O–Co coordination structure lowered the p-band center of the O atom by ∼0.1 eV atom, which passivated lattice-O to improve lattice stability. XPS revealed that the Mn0.6Co2.4O4 material had the maximum built-in potential. The electrochemical testing indicated that it exhibited an overpotential of only 243 mV at a current density of 10 mA cm–2 and maintained stability for over 80 h.
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ANALYTICA CHIMICA ACTA
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2018
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IF 5.123
Feng Tan,Xiao Jiang,Xianliang Qiao,Daming Sun,Jinsuo Gao,Xie Quan,Jingwen Chen,Suyu Ren,Yi Wang
A diffusive gradients in thin films (DGT) method using a new high-capacity cerium oxide (CeO 2 ) binding gel, for the first time, was developed for measuring dissolved inorganic arsenic in freshwater and seawater . The capacities of the new CeO 2 binding gel were 682 μg and 375 μg for As III and As V , respectively. The masses of As III and As V accumulated by CeO 2 -DGT device increased linearly with time and agreed well with the theoretical value calculated by DGT equation. The arsenic accumulation by CeO 2 -DGT was independent of pH (4.05–9.04) and ionic strength (0.1–750 mM), and common anions including CO 3 2− , SO 4 2− , Cl − and PO 4 3− had no obvious interference. CeO 2 -DGT showed excellent long-term deployment performance in freshwater and synthetic seawater . Field trials with CeO 2 -DGT achieved successfully the time-weighted-average concentrations of total inorganic arsenic in reservoir water (1.38 ± 0.09 μg/L) and coastal seawater (0.45 ± 0.06 μg/L). The results were comparable to those measured by grab sampling. The proposed method was reliable and robust for in-situ measurements of dissolved inorganic arsenic in environmental waters.
研究领域:
环境化学
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分析化学
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水环境监测
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环境地球化学
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污染生态学
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2018
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IF 2.831
Chuqi Hou,Wenqin Liu,Zhi Liang,Weichao Han,Jinqing Li,Ling Ye,Menghua Liu,Zheng Cai,Jie Zhao,Yi Chen,Shuwen Liu,Lan Tang
Morusin is a prenylated flavone isolated from mulberry, the branch and root bark of various Morus species, which possesses diverse pharmacological activities. However, it lacks extensive studies about its absorption and disposition. This study investigated the pharmacokinetic behavior of morusin in rat, and its first-pass metabolism in situ . The metabolic pathway of morusin was further investigated by 12 human recombinant UDP-glucuronosyltransferases (UGTs), 9 CYP450s, as well as liver and intestinal microsomes. Four mono-glucuronide metabolites (M-5-G, M-4′-G, M-2′-G, and M II-2 ) were identified in rat intestine and bile by LC–MS/MS, while three of them were also detected in plasma (M-5-G, M-4′-G, and M II-2 ). M-4′-G was the principal conjugate. However, few CYP450 metabolites were found in rat intestine and bile. Only a small amount of M I-1 could be detected in rat plasma. UGT1A1, 1A3, 1A7, and 2B7 were the major contributors to morusin glucuronidation. Morusin exhibited substrate inhibition kinetic characteristics in all UGTs. Clearance rates of M-4′-G in HLM, RLM, UGT1A1, UGT1A3, and UGT2B7 were 137.02, 127.55, 32.54, 41.18, and 35.07 ml/min/mg, respectively. Besides, CYP3A5, 3A4, and 2C19 primarily contributed to the oxidative metabolism of morusin. The pharmacokinetic curves of morusin and its conjugates presented double peaks, showing that an enterohepatic recycling may exist. In conclusion, glucuronidation was confirmed to be the crucial metabolic pathway for morusin in vivo , and M-4′-G was the main metabolite.
研究领域:
药理学
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生物化学
·
药物代谢
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ChemCatChem
·
2020
·Vol. 12
·期 23
·6040-6049
·
IF 4.853
Yongli Sun,Feifei Peng,Luhong Zhang,Bin Jiang,Haozhen Dou,Na Zhang,Mi Xu,Na Yang
Graphical Electrocatalytic Hydrogen Evolution : The morphology and diameter of porous Mo 2 C can be tuned in an easy and precise way. 0D/3D Mo 2 C P/Mo 2 C F exposes abundant active sites and possesses super water affinity to accelerate electrolyte and gas diffusion, and it works better than commercial Pt/C at high current densities in 1.0 M KOH. Herein, a novel 6∼9 nm nitrogen-doped Mo 2 C nanoparticles (Mo 2 C P) homogeneously decorated on 3D porous nitrogen-doped Mo 2 C microflowers (Mo 2 C F) were prepared in situ to form 0D/3D Mo 2 C P/Mo 2 C F. This special structure combines the advantages of Mo 2 C P and porous Mo 2 C F to provide a high density of reaction sites and facilitate the diffusion of electrolyte and hydrogen. Mo 2 C P/Mo 2 C F exhibits excellent HER performance in both alkaline and acidic solutions. It only requires an overpotential of 96 and 118 mV to achieve a current density of 10 mA cm −2 ( η 10 ) in 1.0 M KOH and 0.5 M H 2 SO 4 , respectively. Moreover, it works better than commercial Pt/C at high current densities in 1.0 M KOH. The Tafel slopes are less than 50 mV/dec in both media, suggesting an obviously fast HER kinetics. Additionally, this study presents a universally applicable method to prepare M x C P/Mo 2 C F (M=Fe, Co, V) for different applications.
研究领域:
材料科学
·
电化学
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RARE METALS
·
2024
·Vol. 43
·期 6
·2682-2694
·
IF 9.6
Hu Jie,Lao Hong-Xin,Xu Xiu-Wu,Wang Wei-Kang,Wang Le-Le,Liu Qin-Qin
Though there are numerous intrinsic merits of metal-organic frameworks (MOFs), low charge separation efficiency has imposed heavy restrictions on their photocatalytic application. Herein, in situ porphyrin ligand substitution, as a strategy for improving the charge separation efficiency and increasing the amounts of active sites, has been designed and realized in a Hf-biphenyl dicarboxylic acid (BPDC) MOF. Specifically, a size and geometry matched meso-tetra (4-carboxyphenyl) porphyrin (TCPP) ligand was selected and doped into Hf-BPDC MOF by forming coordinating bonds with Hf centers, forming dual-ligand Hf-BPDC-TCPP MOF. The resultant Hf-BPDC-TCPP MOF showed significantly improved activity and chemical stability in the photocatalytic H 2 generation (261 μmol·g −1 ·h −1 ) and tetracycline (TC) degradation reactions (95.8%), which was 48 and 1.47 folds higher than that of the Hf-BPDC MOF. Photophysical and electrochemical studies revealed that the introduction of porphyrin ligand could generate a stronger internal electric field for boosting the charge separation and transfer, increase the specific surface area for providing more active sites, and narrow the band gap to enhance the visible light absorption. This in situ ligand substitution method provides a promising approach to build a tunable platform for constructing high-performance MOF photocatalysts. Graphical Abstract
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DYES AND PIGMENTS
·
2026
·
IF 3.9
Shengjie Feng,Min Wu,Xiaoqian Yi,Xianggao Li,Hongli Liu,Shirong Wang,Dewang Li
Designed a bipolar monomer V–O-8FTPB with D-A structure and styrene groups. • In-situ crosslinked with TEMPIC during annealing to form a polymer network enabling ambipolar transport. • PCE improved from 20.76% to 23.04%, with only 11% degradation after 3000 h at 60–70% relative humidity (RH). Perovskite solar cells (PSCs) have been developed intensively with the rocketing power conversion efficiency (PCE). However, the moisture and thermal instability primarily hinders their practical application, calling for essential crystal bulk and interface protections. Herein a novel cross linkable bipolar semiconductor material, N,N′-diphenyl-N,N′-bis(4-(4-vinylbenzyloxy)phenyl)-2,2′,3,3′,5,5′,6,6′-octafluoro-1,1′-biphenyl-4,4′-diamine (V–O-8FTPB), was designed for perovskite additive engineering. Specifically, this monomer incorporates styrene terminals for crosslinking, as well as the core of fluorinated biphenyl and triphenylamine as respective n-type and p-type moieties. The crosslinking happened during precursor annealing for perovskite crystallization with the aid of another crosslinking agent tri[2-(3-mercaptopropionyl) ethyl]isocyanurate (TEMPIC). A polymer network wrapping perovskite grains was thus constructed. Such modification on grain boundaries and surfaces endowed defect suppression and reinforced resistance against moisture and further harsh liquid-heat damage. Notably, the bipolar V–O-8FTPB better matched the bipolar transporting of perovskites, neither impeding hole nor electron transport. The optimized photovoltaic device with an active area of 0.09 cm 2 delivered a champion PCE of 23.04%. Furthermore, the encapsulated devices retained 90% of initial efficiency after immersion in water for 72 h, or only 17% decay after 48 h in the 85 °C hot water. This work highlights a novel criteria of bipolar monomer design for in-situ cross-linkable additives to enable the framework charge transport and great hydrophobicity. Download: Download high-res image (335KB) Download: Download full-size image
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ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2026
·
IF 4.6
Shunzi Yu,Chenyi Bao,Nana Liu,Bilai Yan,Zaolong Qu,Shengao Chen,Juan Ni,Xihan Guo,Xian Liu,Han Wang
To investigate how the antioxidants N-Acetyl- L -cysteine (NAC) and Vitamin C (Vc) exert optimal antioxidant effects to mitigate the cytotoxicity induced by titanium dioxide nanoparticles (TiO 2 Nanoparticles, TNPs), this study employed human normal colon epithelial cells (NCM460), human hepatocyte L-02 cells, C57BL/6 mice and Drosophila melanogaster . In NCM460 cells experiment (48 h), results showed that TNPs+NAC co-exposure significantly inhibited malondialdehyde (MDA), enhancing total antioxidant capacity, and reducing chromosomal damage and cell death. This protective sequence was validated in L-02 cells experiment (144 h). In C57BL/6 mice, TNPs+NAC co-exposure reduced the micronucleus frequency in bone marrow polychromatic erythrocytes (PCEs), yet had no significant effect on Morris water maze behavior. Conversely, it adversely affected climbing ability in Drosophila melanogaster . Our study concludes that TNPs + NAC co-exposure effectively mitigates cytotoxicity of TNPs in vitro by enhancing antioxidant effects. However, in vivo outcomes may differ due to factors such as exposure routes and individual variations.
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