CARBOHYDRATE POLYMERS
·
2011
·
IF 3.463
Yuanfeng Wang,Fangfang Mao,Xinlin Wei
The chemical characterization and antioxidant activities of tea leaves polysaccharides (TLPS), tea seed polysaccharide (TSPS), tea flower polysaccharide (TFPS) were investigated after isolated from the leaves, flowers and seeds of camellia sinensis. Results indicated that TLPS, TFPS and TSPS were all composed of Rha, Ara, Gal, Glu, Xyl, GalA and GluA, besides, TLPS was also contained Man and Rib, and TFPS was also contained Man. The molecular weight distribution of TLPS, TFPS and TSPS ranged from 3.67 × 10 3 to 7.58 × 10 5 Da, 2.56 × 10 3 to 1.46 × 10 6 Da and 3.66 × 10 3 to 9.61 × 10 5 Da, respectively. Antioxidant activity tests revealed that TLPS, TFPS and TSPS exhibited strong antioxidation in a concentration-dependent manner. Furthermore, TLPS and TFPS had higher antioxidant activity than TSPS. The nature of monosaccharide and the higher molecular weight in TLPS and TFPS molecules probably contribute to the activities.
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DOI
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Min Mu,Yuelong Wang,Shasha Zhao,Xiaoling Li,Rangrang Fan,Lan Mei,Min Wu,Bingwen Zou,Na Zhao,Bo Han,Gang Guo
Chemotherapy works against tumors by inducing cell apoptosis; however, evasion of apoptosis is recognized to result in resistance to anticancer therapy. Ferroptosis is an iron-dependent cell death pathway that differs from apoptosis in morphological, biochemical, and genetic levels. Combined ferroptosis and apoptosis may shed light on strategies for cancer treatment. Therefore, we have designed a nanoparticle (NP) that can simultaneously cause tumor cell apoptosis and ferroptosis. This NP is composed of epigallocatechin gallate (EGCG) and Fe3+ through a simple and green process and can be used to deliver doxorubicin hydrochloride (DOX) and iron ions to the tumor site at the same time. DOX/Fe3+/EGCG (DF) NPs display a great resolubility and long-term storage stability, and efficient DOX and Fe3+ release is realized after cellular internalization under the high level of glutathione and acidic nature in tumor. EGCG is likely to chemically reduce the released Fe3+ to Fe2+. The generated Fe3+/Fe2+ converts intracellular H2O2 to hydroxyl radicals (•OH) via the Fenton reaction. In addition, the generated •OH subsequently induces lethal ferroptosis to improve DOX-induced apoptosis. In vitro and in vivo investigations indicate that a great therapeutic effect was achieved, suggesting that the formation of the DF NP delivery system is a promising strategy to fight against tumors by an apoptosis and ferroptosis combination modality.
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DOI
PubMed
JOURNAL OF FOOD BIOCHEMISTRY
·
2021
·Vol. 45
·期 5
·e13689
·
IF 2.72
Minghao Fan,Yilin Qian,Wei Yue,Yuqi Yang,Xin Zhang,Shuang Ma,Ying Xu,Dongfeng Wang
The preparation method and the sources of metal elements may affect the activity of the metal–polysaccharide complex. In this study, four Fe–tea polysaccharide complexes were prepared and three tea polysaccharides (TPSs) from different seasons were extracted. Moreover, the binding mode of TPSs with internal and external metallic elements as well as their inhibitory effect on α-glucosidase was explored. The results revealed that the binding mode (–C–O–Fe and –C–Fe) of the Fe–TPS complex prepared at pH 5.0 was closer to TPS with internal metallic elements. The TPS with the least amount of internal metallic elements (61.72 mg/g) exhibited a high inhibitory activity on α-glucosidase (37.90%). The inhibitory activity of Fe–TPS on α-glucosidase was lower than that without Fe. But the quenching effect and the inhibition type of TPSs on α-glucosidase were not affected by metallic elements. Therefore, the metallic elements have the potential to reduce the hypoglycemic activity of TPS. Practical applications In this paper, TPS was extracted from crude tea in different seasons, and the effects of metallic elements in TPS on hypoglycemic activity, physicochemical properties, and structure of TPS were discussed. TPS metal complexes were prepared by adding Fe 3+ or removing metallic elements, and the differences of internal metallic elements in TPS were discussed. It is of great academic significance to use tea pruned leaves and crude tea as potential resources to develop polysaccharide hypoglycemic products and to reveal the relationship between TPS metal ions and their structure and activity. In addition, it has guiding value for consumers to choose tea-producing regions and growers to choose chemical fertilizer.
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Sainan Wang,Pengchang Liu,Yongmei Qin,Zhijun Chen,Jiacong Shen
In this report, a rapid approach for the synthesis of protein conjugated gold nanoclusters under heating condition (AuNCs-protein(p)-heating(h)) was proposed. These AuNCs-p-h were synthesized within 3 min at approximately 100 °C and contain remarkable peroxidase-like activity. Notably, in the presence of tea polyphenols (TPs), the peroxidase-like activity of AuNCs-p-h is restrained, which is likely due to the TP-induced aggregation. A facile approach for TP sensing based on the inhibition effect of TPs towards the peroxidase mimic activity of AuNCs-p-h has been developed. Tea is one of the most popular and health beneficial beverages, the favourable effect of which attributes greatly to its key component, TPs. TP detection thus becomes an important task for various food and beverage companies. This novel TP colorimetric detecting method exhibits a linear response in the concentration range of 10 nM to 10 μM with detection limit of 10 nM as well as prominent selectivity towards various TP related molecules including tyrosol, protocatechuate, chlorogenic acid, theophylline, l -theanine, and l -norepinephrine hydrochloride. Comparing with the traditional tartaric acid colorimetric approach (Tac), this new system shows basically identical experimental results but with much higher sensitivity. AuNCs-p-h can be readily used for TP quantification from real tea samples.
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DOI
高影响因子
AMERICAN JOURNAL OF NEPHROLOGY
·
2023
·
IF 4.2
Shi Heng,Zhao Xinhai,Peng Qin,Zhou Xianling,Liu Sisi,Sun Chuanchuan,Cao Qiuyu,Zhu Shiping,Sun Shengyun
Introduction: Di(2-ethylhexyl) phthalate (DEHP) is a common plasticizer. Studies have revealed that DEHP exposure can cause kidney damage. Green tea is among the most popular beverages in China. Green tea polyphenols (GTPs) have been proven to have therapeutic effects on organ damage induced by heavy metal exposure. However, few studies have reported on GTP-relieving DEHP-induced kidney damage. Methods: C57BL/6J male mice aged 6–8 weeks were treated with distilled water (control group), 1,500 mg/kg/d DEHP + corn oil (model group), 1,500 mg/kg/d DEHP + corn oil + 70 mg/kg GTP (treatment group), corn oil (oil group), and 70 mg/kg GTP (GTP group) by gavage for 8 weeks, respectively. The renal function of mice and renal tissue histopathology of each group were evaluated. The renal tissues of mice in the model, treatment, and control groups were analyzed using high-throughput sequencing. We calculated the differentially expressed microRNAs (miRNAs) and messenger RNAs (mRNAs) using the limma R package, the CIBERSORT algorithm was used to predict immune infiltration, the starBase database was used to screen the miRNA-mRNA regulatory axis, and immunohistochemical analyses were performed to verify protein expression. Results: GTP alleviated the deterioration of renal function, renal inflammation and fibrosis, and mitochondrial and endoplasmic reticulum lesions induced by DEHP in mice. Differential immune infiltrations of plasma, dendritic, T, and B cells were noted between the model and treatment groups. We found that three differentially expressed miRNAs (mmu-miR-383-5p, mmu-miR-152-3p, and mmu-miR-144-3p), three differentially expressed mRNAs (Ddit4, Dusp1, and Snx18), and three differentially expressed proteins (Ddit4, Dusp1, and Snx18) played crucial roles in the miRNA-mRNA-protein regulatory axes when GTPs mitigate DEHP-induced kidney damage in mice. Conclusion: GTP can alleviate DEHP-induced kidney damage and regulate immune cell infiltration. We screened four important miRNA-mRNA-protein regulatory axes of GTP, mitigating DEHP-induced kidney damage in mice
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Huanhuan Li,Shujat Ali,Wenya Wei,Yi Xu,Haowei Lu,Md Mehedi Hassan,Xiangyang Wu,Min Zuo,Qin Ouyang,Quansheng Chen
In this study, a novel fluorescence approach for the rapid and selective detection of organophosphorus pesticides is presented. The strategy is based on inhibition of acetylcholinesterase (AChE) activity and NaY/GdF 4 :Yb, Er up-conversion nanoparticles (UCNPs) fabricated with manganese dioxide (MnO 2 ) nanosheets. Water-soluble UCNPs were first synthesized, and MnO 2 nanosheets were fabricated on their surface to get an efficient quencher for up-conversion fluorescence. Subsequently, AChE was used to restore the fluorescence by dissociation of MnO 2 nanosheets from the surface of UCNPs. When diazinon was added into the system, AChE activity was irreversibly impeded and resulted in the quenching of fluorescence. The inhibitory effect was linearly dependent on the concentration of the pesticide. Under the optimized conditions, the proposed sensor showed excellent performance for the detection of diazinon with a wide detection range of 0.1 to 30 ng/mL and a low detection limit (LOD) of 0.1 ng/mL. Besides, the system showed a good selectivity and was applied for diazinon detection in adulterated tea samples with a recovery rate of 98% to 111%. The unique advantages of the strategy such as simple, cost-effective, highly selective and sensitive make it an attractive approach for the detection of OPs in tea commodities.
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ailong Hu,Zongwei Hao,Xinran Liu,Jinhua Song,Lihua Chu,Daxiang Li,Yiqun Du
Natural biopolymer-based nanoparticles are promising candidates for emulsion stabilization and delivery of hydrophobic compounds. In this study, tea protein (TP) and chitosan (CS) were employed to fabricate complex nanoparticles (NPs) using a pH-dependent method. At a mass ratio of 2:1 (TP:CS), the NPs exhibited favorable properties (206.62 nm, −30.86 mV, 85.41° contact angle) owing to electrostatic interactions, hydrogen bonding, and hydrophobic interactions (binding energy of the complex was −5.40 kcal/mol). At a particle concentration of 2 % and an oil-phase volume fraction of 60 %, the Pickering emulsion stabilized by TP-CS 2:1 NPs remained physically stable. Compared with TP alone, TP-CS 2:1 NPs significantly enhanced the encapsulation efficiency and antioxidant capacity (DPPH/ABTS) of curcumin (Cur). In vitro digestion confirmed that the emulsions reduced gastric degradation of Cur, increasing its bioaccessibility by 17.4 %. Accordingly, this study provides a strategy for designing natural protein-polysaccharide NPs for functional emulsions and sustained delivery systems.
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产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Jiayun Guo,Shuyi Ma,Gege Fan,Ping Ni,Nina Ma,Yinyue Wang,Jiamin Zhu,Heng Wang
In this paper, NdFeO 3 nanofibers were synthesized by the electrospinning method for the first time and used for the detection of triethylamine. XRD, SEM, TEM, BET, XPS, and gas-sensitive test instruments were used to detect the morphology, structure, and gas-sensitive properties of the synthesized products. The results show that the average diameter of the nanorods is about 275 nm. The specific surface area is 10.298 m 2 /g, the total pore volume is 0.112 cm 3 /g, and the average pore diameter is 43.469 nm. Gas sensing tests were carried out on NdFeO 3 nanofibers. The gas-sensitized element was found to have a low optimal operating temperature of 190 °C. The response to 100 ppm triethylamine was 18.9. The response time and recovery time were 72 and 35 s, respectively. A series of test results showed that the sample had excellent selectivity, reproducible detection, long-term stability, and a low detection limit for triethylamine. It has a lot of potential for practical TEA monitoring and detection.
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Fei Zhou,Jinchao Wang,Xuming Jiang,Zhangfeng Zhao,Jiang Shi,Lipeng Hu,Baiyi Lu,Jiyu Peng
Grinding is an essential process in green tea powder production, which greatly affects the final quality. However, in-depth studies on process parameter-quality correlations are lacking, especially regarding the multiple process parameters induced variability. In this work, the influence of process parameters (ambient temperature, rotational speed, and grinding time) on physicochemical and sensory metrics of green tea powder was investigated with univariate and multivariate analysis. Both rotational speed and grinding time contribute to reducing the particle size and water holding capacity (WHC), and increasing lightness, greenness, and yellowness of green tea powder, while low ambient temperature can help to improve the WHC and flowability. Rotational speed is significantly related to the concentrations of gallic acid, catechin, epigallocatechin gallate, and epigallocatechin gallate ( p < 0.01). Rotational speed and grinding time are closely related to the final appearance of green tea powder, while temperature is the dominant factor influencing its aroma.
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产品关联
DOI
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Tianrun Zheng,Yi Lu,Yilin Wang,Hong Zhou,Kang Yang,Mingxue Zhang,Yueying Liu,Fengmin Liu,Geyu Lu
Optimizing and improving the surface activity of Co3O4 sensing materials are crucial for applications in triethylamine detection. Herein, indium-doped Co3O4 hollow microflowers (InCo HMfs), assembled from ultrathin nanosheets, were prepared via a hydrolysis reaction. Three distinct crystal facets─{1 1 0}, {1 1 1}, and {0 0 1}─were observed in 2-InCo HMfs. Results from TEM, XPS, and XAS characterization show that the increase of Co2+ content induced by indium doping promotes the formation of highly active crystal planes in Co3O4. DFT calculations revealed the reasons for the improvement in the gas sensing performance of 2-InCo HMfs and the multiple effects of In ions. Our dual strategy of doping with indium and hydrolysis markedly improved the intrinsic TEA sensing performance of Co3O4, offering a novel avenue for designing high-performance TEA sensors.
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产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 13
·2206
·
IF 5.1
Ying Yu,Zijun Liang,Lei Zhang,Zhizhi Chen,Yixuan Zhao,Qiang Chen,Naixing Ye,Ruxing Yang
To explore the taste characteristics and flavor characteristics of Baiyun 0492 white tea, Fuyun 6 was used as a comparison to describe and analyze the taste of the tea by the standard tea sensory evaluation method and quantitative descriptive analysis. A total of 1083 nonvolatile metabolites were identified by ultrahigh-performance liquid chromatography and mass spectrometry (UPLC–MS–MS). N-Feruloyl tyramine, with strong biological activity, may be the specific metabolite of Baiyun 0492 white tea, and 3,4,5-tricaffeoylquinic acid, with a bitter taste, may be the specific metabolite of Fuyun 6 white tea. Most of the different metabolite contents were negatively correlated with the Baiyun 0492 and Fuyun 6 white tea grades. KEGG metabolic pathway analysis showed that Baiyun 0492 and Fuyun 6 white teas had higher enrichment levels of flavonoids, caffeine, amino acids and carbohydrate-related metabolic pathways. Correlation analysis using widely targeted metabolomics and quantitative taste description showed that most flavonoid differences and some amino acid and phenolic acid differences were significantly positively correlated with bitterness and astringency, including quercetin-3-o-rhamnoside (quercetin), L-arginine, 3,4,5-tricaffioylquinic acid, and neochlorogenic acid. Some soluble sugars were positively correlated with sweetness, including D-maltose and D-sucrose, which were the key taste components of Baiyun 0492 and Fuyun 6. These metabolites may be responsible for the taste characteristics of Baiyun 0492, which is characterized as sweet and mellow, while the taste of Fuyun 6 is mainly mellow. In this study, a wide range of targeted metabolomics techniques were used to screen out different metabolites related to the quality of Baiyun 0492 and Fuyun 6 white teas, providing a reference for clarifying the quality characteristics of Baiyun 0492.
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xiaofang Li,Lingling Guo,Liguang Xu,Liqiang Liu,Maozhong Sun,Xinxin Xu,Hua Kuang,Chuanlai Xu
The European Union (EU) has detected matrine (MAT) in honey and tea exported from China, which is neurotoxic, hepatotoxic, developmentally toxic, and reproductively toxic. To develop a rapid method for the detection of MAT in honey and tea, we designed and analyzed hapten in terms of structure-function similarity, surface electrostatic potential, 3D conformation, and Mulliken atomic charges, prepared a monoclonal antibody (mAb) with high sensitivity (IC 50 = 0.59 μg/L) and specificity to recognize MAT, and developed an immunosensor based on gold nanoparticles (GNP-based immunosensor) for the rapid qualitative detection of MAT in honey and tea samples with visual limit of detection (vLOD) of 2 and 5 μg/kg, respectively. The accuracy of the GNP-based immunosensor was verified by spiking recovery experiments. The GNP-based immunosensor is instrument-free and can be completed within 8 min with the advantages of convenience, rapidity and high sensitivity, which provides a tool for MAT monitoring of honey and tea in China.
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DOI
高影响因子
危险品化学品经营许可证（带存储）